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temperature as a predetermining factor in the development of avena sativa. 193516653270
[effect of microorganismal culture filtrate on production of scopoletin by germinal roots of oat (avena sativa l. )]. 195813534431
atmospheric radiocarbon activity in 1959. 196018988353
studies on the effects of gaseous ions on plant growth. i. the influence of positive and negative air ions on the growth of avena sativa.exposure of avena sativa seedlings to unipolar ionized atmospheres of either charge produced statistically significant stimulation of growth as measured by mean stem length, integral elongation, and dry weight. the extent of growth increase was related to the atmospheric ion density and this in turn determined the magnitude of current flow to ground. the minimal current measured in the ground circuit and capable of producing a measurable difference in growth was 4.3 to 4.6 x 10(-13) amp/plant.196214459883
electrical resistance of cell membranes of avena coleoptiles.the cell membrane resistance to direct current was measured in single cells for the first time in a higher plant tissue, oat coleoptiles (avena sativa). on the assumption that the current density over the cell surface was uniform, a mean value of about 1300 ohm-cm(2) was found for cells in an external nutrient medium containing 1 mmole each of k(+), na(+), and ca(++) per liter. as expected, either decreasing k(+) concentration or increasing ca(++) concentration increased the resistance.196417807848
avenacin, an antimicrobial substance isolated from avena sativa. ii. structure. 196414155109
avenacin, an antimicrobial substance isolated from avena sativa. i. isolation and antimicrobial activity. 196414155108
cross protection and mutual exclusion by three strains of barley yellow dwarf virus in avena sativa l. 19655833319
the fine structure of chloroplast stroma following aldehyde osmium-tetroxide fixation.markedly improved fixation of leaf tissues is obtained by means of a glutaraldehyde (or acrolein)-osmium tetroxide procedure, as compared with the results of potassium permanganate or osmium tetroxide fixation methods. the procedure has proved useful in all species so far examined. chloroplasts are particularly well preserved. in this paper details of components of the ground-substance of avena sativa plastids are presented. they include the following:-(i) the "tromacentre" is an area of aggrega ...196514286298
simulated low-gravity environments and respiratory metabolism in avena seedlings.using horizontal and vertical axis clinostats and sand-grown oat seedlings (avena sativa), it was found that horizontal clinostat rotation at 2 rpm increased respiration and inorganic and organic phosphorus content of seedlings. increased coleoptile geotropism and root growth are attributed to rotational nullification of the directional component of the gravitational stimulus. these growth modifications are mechanistically explicable by the relationship between plant metabolism and auxin concent ...196616656433
phosphorus metabolism of germinating oat seeds.an investigation has been made of the changes in the major phosphorus containing substances in avena sativa during the first 8 days of dark germination. the endosperm, roots, and shoots were analyzed separately for acid soluble-p, phytic acid-p, inorganic-p, lipid-p, nucleic acid-p, and protein-p. phytic acid-p comprised 53% of the total seed phosphate, while the sum of lipid-p, nucleic acid-p and protein-p comprised 27% of the seed phosphate. all these reserve phosphate materials were mobilized ...196616656424
maintenance of polarity of auxin movement by basipetal transport.the polar, basipetal transport of indoleacetic acid helps to maintain polarity of auxin movement in coleoptiles of avena sativa l. by opposing acropetal diffusion. this conclusion is supported by 3 different kinds of experiments. in all 3 experiments, sections took up (14)c carboxyl-labeled indole-3-acetic acid anaerobically, and the distribution of auxin within all sections was similar at the end of uptake.[list: see text].196616656315
movement of indoleacetic acid in coleoptiles of avena sativa l. ii. suspension of polarity by total inhibition of the basipetal transport.acropetal and basipetal movement of indole-3-acetic acid through coleoptiles of avena sativa l. was studied. sections 10-mm long were supplied with either apical or basal sources containing c(14) carboxyl-labeled indoleacetic acid (10(-5)m). anaerobic conditions inhibit metabolically dependent movement (transport) thus reducing basipetal but not acropetal movement. total inhibition of basipetal transport abolishes the polarity of auxin uptake and movement. the nonpolar movement that remains in a ...19665904589
effect of oxygen on photosynthesis, photorespiration and respiration in detached leaves. ii. corn and other monocotyledons.the effect of o(2) on the co(2) exchange of detached leaves of corn (zea mays), wheat (triticum vulgare), oats (avena sativa), barley (hordeum vulgare), timothy (phleum pratense) and cat-tail (typha angustifolia) was measured with a clark oxygen electrode and infrared carbon dioxide analysers in both open and closed systems.corn leaves did not produce co(2) in the light at any o(2) concentration, as was shown by the zero co(2) compensation point and the absence of a co(2) burst in the first minu ...196616656272
mineral ion contents and cell transmembrane electropotentials of pea and oat seedling tissue.the relationships of concentration gradients to electropotential gradients resulting from passive diffusion processes, after equilibration, are described by the nernst equation. the primary criterion for the hypothesis that any given ion is actively transported is to establish that it is not diffusing passively. a test was made of how closely the nernst equation describes the electrochemical equilibrium in seedling tissues. segments of roots and epicotyl internodes of pea (pisum sativum var. ala ...196716656483
metabolic turnover in cell wall constituents of avena sativa l. coleoptile sections. 19676051572
the effects of some auxins on the levels of phosphate esters in avena sativa coleoptile sections. 19676049497
[tannins and growth : iii. analysis of the iaa/tannin-antagonism in oat-coleoptile cylinders].an analysis of the iaa/tannin-antagonism which occurs in oat-coleoptiles was carried out. the investigations were done with 10 mm coleoptile sections of 4-day-old seedlings of avena sativa l. var. "flämingsgold".first it was shown that the iaa/tannin-antagonism which could be established with a tannin-preparation by "fluka" resembled very much that caused by "merck-tannin". whereas the antagonism which was demonstrated by "merck-tannin" seemed to be independent of the iaa concentration, the anta ...196724553745
effect of inversion on growth and movement of indole-3-acetic acid in coleoptiles.the effect of a 180 degrees displacement from the normal vertical orientation on longitudinal growth and on the acropetal and basipetal movement of (14)c-iaa was investigated in avena sativa l. and zea mays l. coleoptile sections. inversion inhibits growth in intact sections (apex not removed) and in decapitated sections supplied apically with donor blocks containing auxin. under aerobic conditions, inversion inhibits basipetal auxin movement and promotes acropetal auxin movement, whereas under ...196716656645
geotropic response of coleoptiles under anaerobic conditions.coleoptiles of avena sativa and zea mays do not develop a geotropic response under anaerobic conditions.196716656622
a cell wall polysaccharide-hydrolyzing enzyme system in avena sativa l. coleoptiles. 19676051573
electrophoretic variation in esterases of three varieties of oats (avena sativa). 19685723612
isolation, properties, and structure of fraction i protein from avena sativa l.1. a method is described for the extraction and purification of fraction i protein from avena sativa l. leaves. 2. the protein possesses ribulose diphosphate carboxylase activity. chromatography on gels of sephadex g-200 separates phosphoribulokinase and ribose phosphate isomerase from the carboxylase. 3. the s°20w was calculated to be 18.2, the stokes radius (determined by gel filtration on a cabibrated column) 74 å, the molecular weight 5.7×10(5), and the frictional ratio 1.35. 4. an amino aci ...196824522874
some properties of phytochrome isolated from dark-grown oat seedlings (avena sativa l.).phytochrome was partially purified from etiolated seedlings of avena sativa l. several properties of the red-absorbing (p(r)) and far-red absorbing (p(fr)) forms of the pigment were compared. the 2 forms could not be shown to differ with respect to their sedimentation velocity in sucrose density gradients, elution volume from sephadex g-200 columns, binding properties on calcium phosphate, or electrophoretic mobility. p(fr), however, was more labile than p(r) during precipitation with 50% ammoni ...196816656907
in vitro phytochrome dark reversion process.thermal reversion of the far-red absorbing form of phytochrome to the red absorbing form in darkness has been investigated in crude and partially purified isolates from a number of etiolated and light grown higher plants. the influence of temperature, aging and urea on the rate of reversion was also determined.phytochrome isolated from all higher plants underwent reversion. the reversion proceeded in at least 2 distinct stages; a short rapid initial phase being followed a slow phase which contin ...196816656838
monovalent ion stimulated adenosine triphosphatase from oat roots.monovalent ion stimulated atpase activity from oat (avena sativa) roots has been found to be associated with various membrane fractions (cell wall, mitochondrial and microsomal) of oat roots. the atpase requires mg(2+) (or mn(+2)) but is further stimulated by k(+) and other monovalent ions. the monovalent ions are ineffective in the absence of the divalent activating cation. the atpase has been described with respect to monovalent ion specificity, temperature, ph, substrate specificity, and mg(2 ...196916657073
[localization of calcium at the level of chloroplasts of oat leaf (avena sativa l) by a very high resolution trace-autoradiography method]. 19694989893
compartments and fluxes of k, na, and cl in avena coleoptile cells.by the compartmental analysis method of macrobbie and dainty, and pitman, estimates of k(+), na(+), and cl(-) concentrations and fluxes were obtained for the cytoplasm and vacuole of coleoptile cells of oat, avena sativa l. cv. victory. double labeling was used in experiments with (42)k plus (22)na and with (42)k plus (36)cl in a complete nutrient solution. at the plasmalemma, according to the ussing-teorell flux ratio equation, na(+) is pumped out and cl(-) is actively transported inward. the r ...197016657527
auxin transport in avena: i. indoleacetic acid-c distributions and speeds.measurements have been made on the initial stages of the transport of carbon-14-labeled indoleacetic acid in the coleoptile of avena sativa l. concentrations of mobile and immobilized indoleacetic acid are related to both distance and time during the first 2 hours after application of the indoleacetic acid at several concentrations to the top of the decapitated coleoptile.at the lowest concentration of indoleacetic acid applied (0.3 mum), the graphs of concentration against distance are linear f ...197016657448
evidence for an electrogenic ion transport pump in cells of higher plants.cyanide (cn) and dinitrophenol (dnp) rapidly depolarize the cells of oat coleoptiles (avena sativa l., cultivar victory) and of pea epicotyls (pisum sativum l., cultivar alaska); the effect is reversible. this indicates that electrogenesis is metabolic in origin, and, since active transport is blocked in the presence of cn and dnp, perhaps caused by interference with atp synthesis, that development of cell potential may be associated with active ion transport. additional evidence for an electrog ...197024174194
dextranase activity in coleoptiles of avena.ani enzyme activity similar to that of dextranase is associated with coleoptiles of avena sativa. when subjected to purified dextranase, both the pure natural dextran and the cell walls of the avena coleoptiles yield isomaltose and isomaltotriose. thus, the cell walls contain dextrans or dextran-like compounds. coleoptiles with low auxin content have a lower dextranase activity than coleoptiles with high auxin content. the enzyme activity is therefore in some way sensitive to thle hormone.197017742615
somatic association in avena sativa l.root tip cells of hexaploid oats avena sativa l. were examined at mitotic metaphase, and distances between homologous as well as between non-homologous chromosomes were measured and their frequency distributions compared. nonhomologous chromosomes were scattered at random in the cells studied. in contrast, the mean distance between homologous chromosomes was significantly shorter. there is a tendency this species. for somatic association of homologs in this species.197017749619
determination of linkages of beta-d-glucans from lupinus albus and avena sativa by exo-beta-(1 --> 3)-d-glucanase. 197016657310
studies on phytochrome. some properties of electrophoretically pure phytochrome.1. phytochrome was purified from etiolated oat (avena sativa) seedlings either by gel-filtration chromatography and ion-exchange chromatography or by gel-filtration chromatography and calcium phosphate chromatography. differences were observed in the spectral properties of phytochrome isolated by the two methods. 2. electrophoresis of pure phytochrome at ph values between 9.0 and 6.0 showed the tendency of phytochrome to form different molecular species. studies in the ultracentrifuge did not sh ...19705499974
colorimetric determination of aconitic acid in avena sativa (oat). 19705530564
microfibril orientation in plant cell walls.the distribution of particles on the surface of the plasmalemma in the collenchyma of apium graveolens was studied by the freeze-etching technique. the aim was to determine whether the distribution of particles was related to the known longitudinal or transverse orientation of cellulose microfibrils in different layers of the walls of these cells. preliminary statistical studies have shown no obvious correlation between particle distribution and microfibril orientation although the distribution ...197024500125
[differences in the light-activation of nadp-dependent glyceraldehyde-3-phosphate dehydrogenase and of ribulose-5-phosphate kinase between plants containing the calvin and those containing the c4-dicarboxylic acid pathway of photosynthetic carbon reduction].1. preceding experiments had shown that irradiance of intact leaves or of isolated chloroplasts causes a reversible increase in the activity of nadp-gpd (ziegler and ziegler, 1965) as well as of ribulose-5-phosphate kinase (latzko and gibbs, 1969). examination of several species which carry out the calvin type of photosynthetic co2 fixation (vicia faba, spinacia oleracea, nicotiana tabacum, avena sativa) now revealed that the dark level of nadp-gpd activity ranges between 300-400 μmol nadph/mg c ...197124493083
solubilization and separation of uridine diphospho-d-glucose: beta-(1 --> 4) glucan and uridine diphospho-d-glucose:beta-(1 --> 3) glucan glucosyltransferases from coleoptiles of avena sativa.the particulate glucan synthetase preparation isolated from a homogenate of oat coleoptiles at 4 c lost 65% of its original activity after 1 day when the udp-d-glucose substrate concentration was 5 x 10(-7)m to 1.0 x 10(-6)m. storage of the particulate enzyme at -20 c or in liquid nitrogen did not prevent the enzyme from losing its activity. incorporation of 0.5% hovine serum albumin into the medium stabilized the particulate enzyme at 0 c for 6 days and for at least 2 weeks in liquid nitrogen.w ...197116657697
induction of coleoptile elongation by carbon dioxide.the ability of co(2) to induce elongation of avena sativa coleoptile segments was examined with the use of a high resolution growth-recording device. co(2)-saturated water causes an 8- to 16-fold promotion in the rate of elongation within 1 minute. this elongation is insensitive to a variety of metabolic inhibitors that suppress auxin-induced elongation, and the co(2) effect cannot be prevented by pretreatment with these inhibitors. buffers of ph 3 to 4 also stimulate elongation quickly, and it ...197116657618
"disaggregation" of phytochrome in vitro-a consequence of proteolysis.the relationship between a large molecular weight (9s) and a small molecular weight (4.5s, 60,000 molecular weight) species of phytochrome was examined to determine if the larger species was an aggregate of the smaller. alterations of ph, salt concentration, or phytochrome concentration did not cause any observable formation of the large form from the small form. however, in partially purified phytochrome extracts from secale cereale l. and avena sativa l., the large form was converted to the sm ...197116657862
effect of avena sativa on cigarette smoking. 19714939551
influence of temperature and seed ripening on the in-vivo incorporation of co(2) into the lipids of oat grains (avena sativa l.).to elucidate the influence of growth temperature and of stage of maturity on lipid synthesis in seeds, oat plants (avena sativa nuda l., variety nos) were fed with (14)co(2) at different stages after flowering, and the (14)c-incorporation into the grain lipids was determined at 2, 24, and 48 hours after the end of (14)co(2)-application. by changing growth temperature from 12 c to 28 c after the application of (14)co(2) to intact plants, a higher (14)c-labeling of saturated fatty acids was found ...197116657814
distribution and activation of the golgi apparatus in geotropism.we find a differential distribution of dictyosomes in the avascular tip cells of the oat (avena sativa) coleoptile upon geostimulation. a differential activation (increased vesicle production) of the dictyosomes with respect to gravity also occurs, but only in the tip cells of the lower tissues. similar differences in distribution and activation of dictyosomes occur also in cells subjacent to the avascular tip (1st and 5th millimeter from the apex) of both the upper and lower half-tissues. when ...197216658015
phosphorylated and nucleotide sugar metabolism in relation to cell wall production in avena coleoptiles treated with fluroride and peroxyacetyl nitrate.coleoptile sections of avena sativa l. were pretreated with sodium fluoride or peroxyacetyl nitrate at levels which inhibit auxin-induced growth but did not affect glucose uptake or co(2) production when postincubated for 30 minutes in a (14)c-glucose medium without auxin. labeling of metabolites involved in cell wall synthesis was measured. peroxyacetyl nitrate decreased labeling, and it was concluded that the pool size of uridine di-phosphoglucose, sucrose, and cell wall polysaccharides decrea ...197216657997
the dark reactions of rye phytochrome in vivo and in vitro.the dark reactions of secale cereale l. cv. balbo phytochrome have been investigated in coleoptile tips and in extensively purified extracts of large molecular weight phytochrome. destruction, but not reversion, was detected in vivo. the effects of various inhibitors of an in vitro phytochrome-degrading protease did not support a view of proteolytic attack as the basis of in vivo destruction. in vitro, rye phytochrome (about 240,000 molecular weight) reverted extremely rapidly, even at 5 c. the ...197216657993
electrical properties of parenchymal cell membranes in the oat coleoptile.parenchymal cells of oat (avena sativa) coleoptiles had an osmotic concentration of 410 mm (determined by plasmolysis); of this only 22 mm was k(+) and 1 mm na(+) (flame photometry). cells were impaled with micropipette electrodes. iontophoretic injection of the dye niagara sky-blue from the micropipette showed that the tip of the electrode penetrated the vacuole. when sections of tissue were immersed in a solution of 22 mm kcl, 1 mm cacl2, and 50 mm glucose, average membrane potential was found ...197224482272
purification of an ion-stimulated adenosine triphosphatase from plant roots: association with plasma membranes.a membrane-bound adenosine triphosphatase (ec 3.6.1.3) that requires mg(++) and that is stimulated by monovalent ions has been purified 7- to 8-fold from homogenates of oat (avena sativa l. cult. goodfield) roots by discontinuous sucrose-gradient centrifugation. the enzyme was substrate specific; adenosine triphosphate was hydrolyzed 25 times more rapidly than other nucleoside triphosphates. the membrane fraction containing adenosine triphosphatase was enriched in plasma membranes, which were id ...197216592027
photoregulation of nicotinamide adenine dinucleotide kinase activity in cell-free extracts.this article gives evidence that nad kinase activity is controlled by the action of phytochrome. the nadp level rapidly increased in the cotyledons of seedlings of pharbitis nil strain violet (a short day plant), when the inductive dark for flowering was interrupted with a 5-minute illumination of red light. illumination with far red light immediately after illumination with red light counteracted partly the effect of the latter.a partially purified phytochrome preparation from the uppermost int ...197216658195
aspects of phytochrome decay in etiolated seedlings under continuous illumination.the rate of total phytochrome decay in the dicotyledons amaranthus caudatus, mirabilis jalapa and pisum sativum under continuous illumination with red, incandescent, and blue light depends on the pfr/ptotal maintained by each source. amaranthus is an exception to this in that there is a deviation from firstorder decay kinetics under continuous illumination with incancdescent light. this deviation is probably not related to the chlorophyll present in the amaranthus sample since chlorophyll-rich p ...197224482270
auxin-induced changes in avena coleoptile cell wall composition.sugar and uronic acid residues were derived from wall polysaccharides of oat (avena sativa, var. victory) coleoptiles by means of 2 n trifluoroacetic acid, 72% sulfuric acid, or enzymic hydrolysis. the products of hydrolysis were reduced and acetylated to form alditol acetates which were analyzed using gas chromatography. time-course studies of auxin-promoted changes in various wall fractions indicate that when exogenous glucose was available, increases in certain wall constituents paralleled in ...197216658216
role of protein synthesis in the senescence of leaves: ii. the influence of amino acids on senescence.when the first leaf of the oat (avena sativa) seedling is detached and placed in the dark, yellowing and proteolysis take place rapidly. the earlier finding that d-serine promotes this process has led to a further study of the controlling roles of several amino acids. since the action of serine was found to be more powerful in presence of kinetin than alone, the effects of other amino acids have been restudied in presence of kinetin. cysteine emerges as a moderately strong promotor of senescence ...197216658191
[experiments on the localisation of flavonoids in plastids. i. flavonoids in etioplasts of avena sativa l]. 19724405256
intracellular distribution of mitochondria after geotropic stimulation of the oat coleoptile.the number of mitochondria is greater in the bottom than in the top of cells in geotropically stimulated oat (avena sativa) coleoptiles. in the avascular tip and outer epidermis of subapical regions this difference occurs only in the lower tissues. these inequalities are found both in the kmno(4) (-) and in the glutaraldehyde-fixed tissues; however, they are significant only in the former. also, the number of mitochondria scored is consistently lower when the tissues were fixed in kmno(4). these ...197216658131
isolation of protoplasts from cereal leaves.mature leaves of secale cereale cut into narrow strips and incubated for 18 h in a mixture of cellulase (meicelase) and pectinase (pectinol r10) produced quantities of protoplasts. under the same conditions leaves of triticum aestivum, hordeum vulgare and avena sativa also produce protoplasts but in lower yields. the wheat and rye protoplasts in culture appear to regenerate a cell wall but only a very small proportion undergo cell division.197224481701
photoconversion of riboflavin to lumichrome in plant tissues.free flavins have been extracted from shoots of etiolated corn (zea mays l., var. burpee snowcross) and from yeast cells and separated from other substances by absorption on resorcinol-formaldehyde resin and talc columns and by thin layer chromatography. riboflavin was the only free flavin present. extracts of etiolated shoots of oats (avena sativa l., var. multiline e-69 and clinford) yielded riboflavin plus a second free flavin previously demonstrated in oats. the areas of the chromatograms ex ...197216658098
characterization of plasma membrane-associated adenosine triphosphase activity of oat roots.atpase activity of plasma membranes isolated from oat (avena sativa l. cv. goodfield) roots was activated by divalent cations (mg(2+) = mn(2+) > zn(2+) > fe(2+) > ca(2+)) and further stimulated by kcl and a variety of monovalent salts, both inorganic and organic. the enzyme exhibited greater specificity for cations than anions. the presence of mg(2+) was necessary for kcl stimulation. ca(2+) was ineffective in replacing mg(2+) for activation of plasma membrane atpase, but it did activate other m ...197316658500
the lateral transport of iaa in intact coleoptiles of avena sativa l. and zea mays l. during geotropic stimulation.movement of iaa was studied in excised coleoptile apices and whole seedlings of zea mays l. and avena sativa l. during geotropic stimulation. a micropipette technique permitted the application of [5-(3)h]iaa at predetermined points on the coleoptiles with minimal tissue damage.when [5-(3)h]iaa was applied to the upper side of a horizontal excised zea coleoptile, about 60% of the recoverable radioactivity had moved into the lower half after 2 h. in contrast, when application was made to the lower ...197324458860
substrate activation of beta-(1 --> 3) glucan synthetase and its effect on the structure of beta-glucan obtained from udp-d-glucose and particulate enzyme of oat coleoptiles.udp-d-glucose, at a micromolar level in the presence of mgcl(2) and oat (avena sativa) coleoptile particulate enzyme which contains both beta-(1 --> 3) and beta-(1 --> 4) glucan synthetases, produces glucan with mainly beta-(1 --> 4) glucosyl linkages. an activation of beta-(1 --> 3) glucan synthetase by udp-d-glucose and a decrease in the formation of beta-(1 --> 3) glucan in the presence of mgcl(2) have been observed. however, at high substrate concentration (>/= 10(-4)m), the activation of be ...197316658488
on the nature of the physiological responses of avena stem segments to gibberellic acid treatment.gibberellic acid was found to cause elongation in avena sativa (oat) stem segments whether it was applied continuously or as a short pulse. the shorter the pulse time became, the higher was the gibberellic acid concentration needed to cause elongation; the segmental growth apparently depends upon the amount of gibberellic acid taken up by the segments. avena segments showed a decreased growth response to gibberellic acid if the treatments were initiated at increasingly later times after excision ...197316658458
somatic association of chromosomes in asynaptic genotypes of avena sativa l. 19734712858
inhibition of low ph-induced elongation in avena coleoptiles by abscisic acid.an angular position-sensing transducer was used to make continuous measurements of acid-induced elongation of avena sativa coleoptile segments. elongation rates at ph 4.5 (5 mm succinate buffer) were about 5-fold greater than those at ph 6.0. buffered 0.1 mm abscisic acid produced a partial decrease of the growth rate. pretreatments with abscisic acid buffered at ph 6.0 usually caused a further reduction of the elongation response when the coleoptile segments were subsequently placed in buffer a ...197316658443
membrane-bound adenosine triphosphatase activities of oat roots.homogenates of oat (avena sativa cv. goodfield) roots contained at least five membrane-associated adenosine triphosphatase (atpase) activities. the membrane-bound atpases were separated on sucrose gradients and distinguished by membrane density, ph optima, sensitivity to monovalent salts, and substrate specificity.a membrane fraction sedimenting at low centrifugal force (13,000g) contained two atpase activities at ph 9.0. one membrane atpase was coincident with cytochrome c oxidase activity and ...197316658403
phytochrome destruction: an apparent requirement for protein synthesis in the induction of the destruction mechanism.examination of the phytochrome destruction reaction as a function of age in etiolated oat (avena sativa l. cv. garry) seedlings demonstrates that following illumination of 3-day-old shoots there is a lag, not observed in 4- or 5-day-old oats, prior to the onset of destruction. this light-mediated induction of the phytochrome destruction mechanism in 3-day-old shoots is inhibited by chloramphenicol, actinomycin d, and puromycin suggesting that protein synthesis is required. in 4-day-old shoots, a ...197316658553
production of aneuploids in avena sativa l. 19734762793
polarity and rate of transport of cyclic adenosine 3,5'-monophosphate in the coleoptile.transport of tritiated cyclic amp in the coleoptile of oats (avena sativa) and corn (zea mays) is polar, with basipetal to acropetal ratios of 4.0 and 3.2, respectively. the rate of transport is approximately that of indoleacetic acid. the linear velocity of transport, however, is at least five times that of auxin. a loss in transport polarity of the nucleotide occurs in subapical tissues within several hours after decapitation of the coleoptile, accompanied by a decrease in transport rate. the ...197316658508
purification of oat and rye phytochrome.a purification procedure employing normal chromatographic techniques is outlined for isolating phytochrome from etiolated oat (avena sativa l.) seedlings. yields in excess of 20% (25 milligrams or more) of phytochrome in crude extract were obtained from 10- to 15-kilograms lots. the purified oat phytochrome had an absorbance ratio (a(280) nm/a(665) nm) of 0.78 to 0.85, comparable to reported values, and gave a single major band with an estimated molecular weight of 62,000 on electrophoresis in s ...197316658440
rhythmicity in the basipetal transport of indoleacetic acid through coleoptiles.(14)c-indoleacetic acid was applied to coleoptiles of corn (zea mays) and oat (avena sativa). the coleoptiles were detached from the endosperms at 6-minute intervals after indoleacetic acid application, and the radioactivity was determined in successive 2-millimeter regions. the rate (per cent per minute) of basipetal transport of indoleacetic acid is periodic in various regions of the coleoptile, with a period of about 20 minutes. the possible relation of this cyclic phenomenon to other rhythmi ...197316658381
kinetics of stress relaxation properties of oat coleoptile cell wall after geotropic stimulation.this study describes the stress relaxation of the cell wall of oat (avena sativa) coleoptiles after different periods of geotropic stimulation. the upper and lower tissues (with respect to gravity) of geotrophically stimulated coleoptiles exhibit different wall properties. the lower tissues are less resistant to deformation than the upper. the ratio of stress to strain is significatly less in the lower than in the upper tissue. similarly, the relaxation time and the minimum relaxation time, deri ...197316658352
rapid growth inhibition of avena coleoptile segments by abscisic acid.an angular position sensing transducer was used to make continuous measurements of elongation of a column of avena sativa coleoptile segments. elongation stimulated by 2 mum indoleacetic acid was inhibited by 0.1 mm abscisic acid with a latent period of about 4 or 5 minutes at ph 6.0, 30 c. full growth inhibition was not established until about 1 hour after the addition of the abscisic acid. the same degree of final growth inhibition could be obtained under the above conditions using 10 mum and ...197316658304
comparative immunochemistry of phytochrome.partially purified high molecular weight preparations of phytochrome, estimated to be close to 440,000 molecular weight based upon chromatography through a calibrated bio-gel p-300 column, were obtained from garry and newton oats (avena sativa l., cv. garry and cv. newton), rye (secale cereale l., cv. balbo), barley (horedum vulgare l., cv. harrison), and pea (pisum sativum l., cv. alaska) by a sequence of three chromatographic steps: brushite, diethylaminoethyl cellulose, and bio-gel p-300. no ...197316658285
letter: treatment of nicotine addiction with avena sativa. 19744471694
multiple forms and intracellular localization of uridine diphosphate glucose pyrophosphorylase in avena sativa.uridine diphosphate glucose pyrophosphorylase was isolated separately from avena sativa leaves, roots, and etiolated coleoptiles and purified by ammonium sulfate fractionation, deae-sephadex chromatography and polyacrylamide gel electrophoresis. there was no difference in the enzyme from the different tissue types with respect to properties exhibited during the purification procedure. a small portion of the enzyme from all three sources was found to be particulate when homogenized in aqueous suc ...197416658857
changes in plastid envelope polypeptides during chloroplast development.a quantitative estimation of sodium dodecyl sulphate-extractable plastid envelope polypeptides during greening of avena sativa l. laminae is described, combined with protein distribution and plastid number studies over the same period.a primary light-dependent and cytoplasm-dependent increase in both total plastid protein and envelope associated protein during the first 30 minutes of greening was observed, followed by a period during which release of envelope-associated protein either into the p ...197424442806
activation of avena coleoptile cell wall glycosidases by hydrogen ions and auxin.several cell wall-bound glycosidases present in avena sativa coleoptiles were assayed by following the hydrolysis of p-nitrophenyl-glycosides. particular emphasis was placed on characterizing some parameters affecting the activity of beta-galactosidase. the ph optimum of this enzyme is 4.5 to 5.5; it is sensitive to copper ions and p-chloromercuribenzoate treatment and apparently has an exceptionally low turnover rate. indoleacetic acid treatment enhanced in vivo beta-galactosidase activity of c ...197416658680
iaa transport during the phototropic responses of intact zea and avena coleoptiles.transport of indolyl-3-acetic acid (iaa) was studied during the phototropic responses of intact shoots and detached coleoptiles of zea mays l. and avena sativa l. the use of a high specific activity [5-(3)h]iaa and glass micropipettes enabled asymmetric application of the iaa to be made to individual coleoptiles with minimal tissue damage.a unilateral stimulus of 2.59×10(-11) einstein cm(-2) of blue light, probably in the dose range of the first positive phototropic response, caused significant ...197424442803
subcellular localization of the red-absorbing form of phytochrome by immunocytochemistry.an immunocytochemical technique was used to localize the red-absorbing form of phytochrome at the light- or electron-microscope level in etiolated barley (hordeum vulgare l.) coleoptile tip, rice (oryza sativa l.) coleoptilar node, maize (zea mays l.) coleoptile tip, rye (secale cereale l.) coleoptile tip and coleoptilar node, and oat (avena sativa l.) root cap. staining for phytochrome in the cells was found to be generally distributed throughout the cytoplasm. in addition, barley also showed s ...197424442776
iron requirement and iron uptake from various iron compounds by different plant species.the fe requirements of four monocotyledonous plant species (avena sativa l., triticum aestivum l., oryza sativa l., zea mays l.) and of three dicotyledonous species (lycopersicum esculentum mill., cucumis sativus l., glycine maxima (l.) merr.) in hydroponic cultures were ascertained. fe was given as nafe-eddha chelate (fe ethylenediamine di (o-hydroxyphenylacetate). i found that the monocotyledonous species required a substantially higher fe concentration in the nutrient solution in order to att ...197416658933
phytochrome: immunocytochemical assay of synthesis and destruction.the protein moiety of phytochrome, when assayed by an immunocytochemical technique, was not detected in dry oat (avena sativa l., cv. garry) and rye (secale cereale l., cv. balbo) grains or in grains hydrated 4 h at 25°. after 24 h of hydration immunochemical activity of phytochrome in situ was readily detectable. immunocytochemical assays of destruction show that different regions of 3-day-old, single oat and rye seedlings have different destruction kinetics. oat roots apparently exhibit much s ...197424442499
evidence against the occurrence of adenosine-3':5'-cyclic monophosphate in higher plants.previous reports on the incorporation of [(14)c]adenine into adenosine-3':5'-cyclic monophosphate (cyclic amp) in oat (avena sativa l.) and maize (zea mays l.) coleoptile sections, chick-pea (cicer arietinum l.) embryos and barley (hordeum vulgare l.) aleurone layers were reexamined. separation of labelled nucleotides on deae-sephadex a 25 showed that a peak of (14)c activity, previously considered to be cyclic amp, is not identical with this compound. attempts to detect the cyclic nucleotide by ...197424442328
concentrations of indole-3-acetic acid and its esters in avena and zea.an isotope-dilution method has been developed for the assay of free indole-3-acetic acid and ester indole-3-acetic acid as measured by indole-3-acetic acid liberated by mild alkaline hydrolysis. application of this method to seedlings of avena sativa and zea mays indicates the upper limit of free indole-3-acetic acid in avena to be about 16 mug per kg and in zea, about 24 mug. the amount of 1 n alkali-labile indole-3-acetic acid in zea is about 330 mug per kg and there is very little 1 n alkali- ...197416658870
effects of indoleacetic acid on dictyosomes of apical and expanding cells of oat coleoptiles.we found that the auxin-induced growth is mediated through the activation of the dictyosomes (collectively, the golgi apparatus). incubation of oat (avena sativa) coleoptile segments in indoleacetic acid-sucrose-phosphate buffer changes significantly the number of dictyosomes in the expanding cells. a further indication of auxin enhancement of dictyosome activity is a decrease in dictyosomal cisternae (flattened membranous sacs) number. this decrease occurred after 6 minutes of incubation in aux ...197416658863
proceedings: on the pharmacology of an extract of avena sativa. 19744451796
lack of effect of avena sativa on cigarette smoking. 19744431518
stimulation of avena coleoptile growth by reduction of oxygen supply.the growth of avena sativa l. coleoptiles was accelerated by reduction of the o2 concentration in the surrounding atmosphere. cell-wall extensibility was increased in close relation to the increase in elongation, 6-8% o2 giving the optimal effect in either case. growth promotion by reduced o2 concentration and by auxin (indole-3-acetic acid; iaa) were additive, at least at lower auxin concentrations.this response to reduced o2 concentration was also present in sections pretreated with cyclohexim ...197424458929
effect of ph and auxin on chloride uptake into avena coleoptile cells.the effect of ph on (36)cl(-) movement into coleoptile cells (avena sativa l. cv. garry) was investigated and compared with effects of indoleacetic acid. (36)cl(-) uptake, but not efflux, is stimulated when coleoptile sections are placed in media adjusted to ph levels from 5 to 3 after a preincubation period at ph 6.5. the enhancement is seen within 2 minutes, is not correlated with growth, and is completely erased by respiratory inhibitors. in comparison to the acid-induced stimulation, the sti ...197416658985
the metabolism of oat leaves during senescence: i. respiration, carbohydrate metabolism, and the action of cytokinins.when the detached first leaves of green or etiolated oat (avena sativa cv. victory) seedlings senesce in the dark, their oxygen consumption shows a large increase, beginning after 24 hours and reaching a peak of up to 2.5 times the initial rate by the 3rd day. this effect takes place while the chlorophyll of green leaves, or the carotenoid of etiolated leaves, is steadily decreasing. kinetin, at a concentration which inhibits the decrease in pigment, completely prevents the respiratory rise; ins ...197416658877
effect of red light on coleoptile growth.the effects of red light in reducing the growth of the oat (avena sativa l.) coleoptile and the synthesis of auxin in the coleoptile tip are detectable 2 hours after treatment and become more pronounced with time. when the coleoptile tip is supplied with additional tryptophan the synthesis of auxin is doubled both in darkness and when exposed to red light. treatment of the tip with gibberellic acid or pyridoxal phosphate overcomes the reduction of auxin synthesis caused by red light. the uptake ...197416658875
the influence of 0.03% carbon dioxide on protein metabolism of etiolated avena sativa coleoptiles.the influence of indoleacetic acid, 0.03% co(2), and malate on protein metabolism of etiolated avena sativa coleoptile sections has been investigated. all three were found to elevate both the rate of incorporation of labeled leucine into protein, and the level of soluble protein. the combination of indoleacetic acid and co(2) stimulated these values in an additive or weakly synergistic manner, in contrast to the nonadditive influence of malate and co(2). evidence is presented that cyclo-heximide ...197416658830
a synergistic stimulation of avena sativa coleoptile elongation by indoleacetic acid and carbon dioxide.the ability of 0.03% co(2) to stimulate growth has been investigated using etiolated avena coleoptile sections maintained in buffered solution. this concentration of co(2)-stimulated growth after a lag period of 12 to 15 minutes, and a synergistic relationship between indoleacetic acid and co(2) in stimulating growth has been demonstrated. the response to co(2) is inhibited by cycloheximide and is lost approximately 10 minutes after exposure to co(2)-free air. malate can replace co(2) in stimula ...197416658829
the effect of abscisic acid on the uptake of potassium and chloride into avena coleoptile sections.the effect of abscisic acid (aba) on uptake of potassium ((86)bb(+) or (42)k(+)) by avena sativa l. coleoptile sections was investigated. aba lowered the potassium uptake rate within 30 min after its application and inhibition reached a maximum (ca. 75%) after 2 h. the inhibition of k(+) uptake increased with aba concentration over a range of 0.03 to 10 μg/ml aba. at a higher k(+) concentration (20 mm) the percentage inhibition decreased. the percentage inhibition of k(+) uptake by aba remained ...197424458128
the use of an enzyme electrode in the analysis of indole-3-acetic acid oxidase activity in avena.a flexible analytical system which allows for the continuous potentiometric monitoring of the disappearance of an electrochemical species, ferrocyanide, by the peroxidase enzyme is described. the ability of peroxidase to mediate the oxidation of indole-3-acetic acid is followed by observing the competition of indole-3-acetic acid with ferrocyanide for the peroxidase enzyme. this is accomplished by examining potentiometrically the decrease in the rate of ferrocyanide oxidation with increasing ind ...197416658775
amyloplast size and number in gravity-compensated oat seedlings.gravity compensation by the horizontal clinostat increases the diameter of amyloplast starch grains of oat (avena sativa cv. victory) coleoptile parenchyma cells, as compared to vertically rotated and stationary controls. in dark-grown coleoptile tip parenchyma cells, measured starch grain sizes exhibit a wide distribution of diameters, from approximately 1.5 to approximately 8.0 mum, but fall into three prominent diameter classes. the compensated tissues from both the tip and the subapical regi ...197416658713
phytochrome stability in vitro: i. effect of metal ions.photoreversible phytochrome disappears from etiolated tissue upon actinic irradiation. such disappearance, of possible physiological importance, involves several processes, at least one of which is accelerated by metals in vivo. purified phytochrome from oat (avena sativa l. cv. garry) coleoptiles is greatly stabilized in vitro by scrupulous removal of metal impurities via chelating agents. such stabilized phytochrome decays rapidly upon the addition of about 10 mum hg(2+), cd(2+), cu(2+), and z ...197416658705
use of protein in extraction and stabilization of nitrate reductase.the in vitro instability of nitrate reductase (ec 1.6.6.1) activity from leaves of several species of higher plants was investigated. decay of activity was exponential with time, suggesting that an enzyme-catalyzed reaction was involved. the rate of decay of nitrate reductase activity increased as leaf age increased in all species studied. activity was relatively stable in certain genotypes of zea mays l., but extremely unstable in others. in all genotypes of avena sativa l. and nicotiana tabacu ...197416658769
phytochrome characterization by rabbit antiserum against high molecular weight phytochrome.both small and large sizes of phytochrome purified from garry oat (avena sativa l. ev. garry) as well as large phytochrome purified from newton oat (a. sativa l. cv. newton), rye (secale cereale l. cv. balbo), barley (hordeum vulgare l. cv. harrison), and pea (pisum sativum l. cv. alaska) seedlings are characterized by a specific antiserum against large garry oat phytochrome. a spur is observed by double diffusion assay against large and small garry oat phytochrome indicating only partial identi ...197516659052
the limit dextrinases from ungerminated oats (avena sativa l.) and ungerminated rice (oryza sativa l.).the limit dextrinases from ungerminated oats and rice have been purified, and their substrate specificity compared with a bacterial isoamylase preparation. both cereal enzymes could hydrolyse (1 yields6)-alpha-d-glucosidic linkages in oligosaccharide alpha-dextrins, pullulan, amylopectin, and the beta-limit dextrins of amylopectin and glycogen. however, under comparable conditions, they were unable to attack glycogens.19751170015
the pharmacology of avena sativa.the pharmacology of avena sativa has been investigated in laboratory animals following a report that tincture of avena sativa reduced the craying for cigarettes in man. the tincture, evaporated to dryness, craving for cigarettes in man. the tincture, evaporated to dryness, re-constituted in an equal volume of water and administered by stomach tube or intraperitoneal injection, antagonized the antinociceptive effect of morphine in two separate test (hot-plate and tail flick). compared with animal ...1975237083
evidence for ammonium-dependent de novo synthesis of glutamate dehydrogenase in detached oat leaves.glutamate dehydrogenase becomes density labeled through the incorporation of deuterium and (15)n when detached oat leaves (avena sativa var. fulghum) are incubated in the presence of ammonia. the enzyme has been isolated by means of deae-cellulose chromatography, ammonium sulfate precipitation, isopycnic equilibrium centrifugation, and disc electrophoresis from leaves fed l-methionine-(35)s. radioactivity is incorporated into isozyme 1 of glutamate dehydrogenase, whereas isozyme 2, detected only ...197516659411
regulation of cell wall synthesis in avena stem segments by gibberellic acid.gibberellic acid induces (a) increased elongation of avena sativa stem segments, (b) increased formation of cell wall material, measured on the basis of dry weight, and (c) increased incorporation of (14)c-glucose into all fractions of the cell wall material. this increased incorporation of radioactivity correlates well with increased formation of cell wall material and shows a time-course pattern similar to the time course of the elongation response. approximately one hour after the application ...197516659206
effects of magnesium, calcium and lanthanum ions on stomatal oscillations in avena sativa l.mg(2+), ca(2+) and la(3+) caused an increased period time of the transpiratory oscillations when given to excised oscillating avena plants (plants without root system). the effect was reversible, i.e. after withdrawal of the ions the period time returned to its original value. in order to achieve the same period lengthening as with 2.5 mm la(3+), 20 mm ca(2+) and 40 mm mg(2+) was needed. the effects are discussed along two lines: (a) the ions interfere with ionic processes, central for the stoma ...197524435179
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