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[research advances in pharmacology of aucubin and aucubigenin].the advances in the research on pharmacological activities of aucubin have been summarized in the last ten years. aucubin is one of active components of chinese medicinal herbs such as eucommia ulmoides and has been shown wide pharmacological activities including hepatoproective, antitoxicanti-inflammatory, antioxidant, antiaging, antiosteoporosis and neurotrophic and should be further researched and utilized.200718338591
du-zhong (eucommia ulmoides oliver) leaf extract mediates hypolipidemic action in hamsters fed a high-fat diet.this study examined the effect of a du-zhong (eucommia ulmoides oliver) leaf extract (0.175 g/100 g diet) that was supplemented with a high-fat diet (10% coconut oil, 0.2% cholesterol, wt/wt) on hyperlipidemic hamsters. hamsters fed with du-zhong leaf extract for 10 weeks showed a smaller size of epididymal adipocytes compared to the control group. the supplementation of the du-zhong leaf extract significantly lowered the plasma levels of triglyceride, total cholesterol, ldl-cholesterol, non hdl ...200818306452
[effects of calcium phosphate cement/eucommia ulmoides extracts compound on cell proliferation and adhesion of mc3t3e1 osteoblasts in vitro].to observe the effects of calcium phosphate cement/eucommia ulmoides extracts compound (cpc/eue) on cell proliferation and adhesion of mc3t3e1 osteoblasts in vitro.200818275124
[research on the quality changes in pre- and post-processed pieces of eucommia ulmoides].to study the quality changes in pre- and post-processed pieces of eucommia ulmoides oliv.200717727054
iridoids from the green leaves of eucommia ulmoides.the bark of eucommia ulmoides is a well-known crude drug in oriental medicine, and its leaves have been consumed as a beverage. from the green leaves of this plant, three new iridoids (1-3) were isolated, together with 12 known compounds. compound 1 is the first iridoid possessing a saturated bond between c-3 and c-4 and having an ether linkage between c-3 and c-2 of the glucose unit. furthermore, 2 and 3 may be regarded as the first naturally occurring conjugates of an iridoid and an amino acid ...200717691745
[determination of geniposidic acid and chlorogenic acid in male flowers and related products of eucommia ulmoides by reversed-phase high performance liquid chromatography].a simple and rapid high performance liquid chromatographic method has been developed for the determination of geniposidic acid and chlorogenic acid in the male flowers and related products of eucommia ulmoides. two components were separated by a shim-pack vp-ods column (150 mm x4.6 mm, 5 [microm) with a mobile phase of methanol-water-acetic acid (24 :75: 1, v/v) at a flow rate of 1 ml/min, column temperature of 30 93 and detection wavelength of 240 nm. under the chromatographic conditions mentio ...200717580690
[content comparison of pinoresinol diglucoside in original and reborn bark of eucommia ulmoides].to evaluate the reborn barks quality, antihypertensive effective component of pinoresnol diglucoside (pdg) in three times reborn barks were determined.200617076238
abp1 expression regulated by iaa and aba is associated with the cambium periodicity in eucommia ulmoides oliv.a cdna clone of eucommia ulmoides oliv. encoding auxin binding protein 1 (abp1), one of the putative receptors of auxin, was isolated, and the seasonal expression of abp1 in relation to iaa and aba annual variation was investigated by different technical approaches including rt-pcr, real-time pcr, northern blotting, western blotting, and immunolocalization. in the cambial region, abp1 expression at both the protein and the mrna level was found to be high, low, and remarkably scarce in the active ...200617030542
[isolation, purification and identification of geniposidic acid in eucommia ulmoides oliv. by silica column chromatography, reversed-phase high performance liquid chromatography, liquid chromatography-electrospray ionization mass spectrometry and nuclear magnetic resonance].the methods using silica column chromatography, reversed-phase high performance liquid chromatography, liquid chromatography-electrospray ionization mass spectrometry and nuclear magnetic resonance (rp-hplc/lc-esi-ms/nmr) for the isolation, purification and identification of geniposidic acid (gpa) in eucommia ulmoides oliv. were established. the crude extract of e. ulmoides was obtained after e. ulmoides was extracted with 70% ethanol and the extract was concentrated under reduced pressures. the ...200516350801
separation of polyprenol and dolichol by monolithic silica capillary column chromatography.we attempted an analysis of naturally occurring polyprenol and dolichol using a monolithic silica capillary column in hplc. first, the separation of the polyprenol mixture alone was performed using a 250 x 0.2 mm inner diameter (id) octadecylsilyl (ods)-monolithic silica capillary column. the resolution of the separation between octadecaprenol (prenol 18) and nonadecaprenol (prenol 19) exceeded by >or=2-fold the level recorded when using a conventional ods-silica particle-packed column (250 x 4. ...200516061949
[study on technique of extraction and purifying chlorogenic acid in eucommia ulmoides oliver leaves].ethanol was used as solvent to extract chlorogenic acid from leaves of eucommia ulmoides oliver leaves. the optimum conditions are obtained by response surface analytical method. optimized conditions for the extraction of chlorogenic acid were obtained: ethanol 51.7%, extraction temperature 55.8 degrees c, ratio of material to liquor 1:12.8, extraction time 2 h. the static adsorption tests are conducted among six kinds of macroporous resins to select nka-ii resin as a optimum resin to adsorb the ...200415807249
[isolation and purification of three iridoids from eucommia ulmoides oliv. with stepwise gradient preparative liquid chromatography]. 200415712886
opposite patterns in the annual distribution and time-course of endogenous abscisic acid and indole-3-acetic acid in relation to the periodicity of cambial activity in eucommia ulmoides oliv.the seasonal change of free abscisic acid (aba) and indole-3-acetic acid (iaa) and their relationship with the cambial activity in eucommia ulmoides trees were investigated by aba and iaa immunolocalization using primary polyclonal and rhodamine-red fluorescing secondary antibodies, aba and iaa quantification using high performance liquid chromatography (hplc), and systematic monitoring of vascular cell layers production. aba and iaa clearly displayed opposite annual distribution patterns. in th ...200515710633
[studies on chemical constituents from eucommia ulmoides oliv].to study the chemical constituents from eucommia ulmoides oliv.200415376388
crystal structure of a novel antifungal protein distinct with five disulfide bridges from eucommia ulmoides oliver at an atomic resolution.eafp2 is a novel antifungal protein isolated from the bark of the tree eucommia ulmoides oliver. it consists of 41 residues and is characterized with a five-disulfide motif and the inhibitory effects on the growth of both cell wall chitin-containing and chitin-free fungi. the crystal structure of eafp2 at an atomic resolution of 0.84 a has been determined by using shake-and-bake direct methods with the program snb. the phases obtained were of sufficient quality to permit the initial model built ...200415363789
solution structure of eucommia antifungal peptide: a novel structural model distinct with a five-disulfide motif.the three-dimensional structure in aqueous solution of eucommia antifungal peptide 2 (eafp2) from eucommia ulmoides oliv was determined using (1)h nmr spectroscopy. eafp2 is a newly discovered 41-residue peptide distinct with a five-disulfide cross-linked motif. this peptide exhibits chitin-binding activity and inhibitory effects on the growth of cell wall chitin-containing fungi and chitin-free fungi. the structure was calculated by using torsion angle dynamic simulated annealing with a total o ...200415147184
induction of apoptosis in hl-60 cells treated with medicinal herbs.in order to develop a new apoptosis inducer, we screened 22 crude drugs for their apoptosis-inducing activity. it was found that glycyrrhiza uralensis, cynomorium songaricum, eucommia ulmoides, phellodendron amurense, cinnamomum cassia and paeonia lactiflora induced the death of hl-60 cells. to investigate the mechanism of apoptosis induced by these six crude drugs, the mitochondrial transmembrane potential and the activity of caspase-3 were measured. reduced mitochondrial transmembrane potentia ...200314587878
study on the inhibitory effects of korean medicinal plants and their main compounds on the 1,1-diphenyl-2-picrylhydrazyl radical.a 1,1-diphenyl-2-picrylhydrazyl (dpph) radical-generating system was used to evaluate the antioxidant properties of korean medicinal plants that have been used widely as folk medicines for several disorders, as well as compounds isolated from them. among the rosaceae, rosa rugosa and rosa davurica showed strong dpph radical-scavenging activity. the most effective medicinal plant from families other than rosaceae was cedrela sinensis, followed in order by nelumbo nucifera, eucommia ulmoides, zant ...200313678241
[advances in the study of chemical constituents and pharmacology of eucommia ulmoides oliver]. 200312964557
[effects of growth regulator, full exposure and mist spray on the cutting of eucommia ulmoides].to study the effects of growth regulator on the cutting method of eucommia ulmoides seedlings.200112901096
relationship between endogenous indole-3-acetic acid and abscisic acid changes and bark recovery in eucommia ulmoides oliv. after girdling.eucommia ulmoides (eucommiaceae), a traditional chinese medicinal plant, is often subjected to severe manual peeling of its bark. if the girdled trunk is well protected from desiccation, new bark forms within 1 month. it has been proposed that phytohormones play a key role in this process. research has been conducted to determine the distribution of endogenous indole-3-acetic acid (iaa) and abscisic acid (aba) during the bark recovery, using high-performance liquid-chromatography (hplc) and fluo ...200312869522
[studies on the phenylpropanoids from eucommia ulmoides].to study the chemical constituents from the leaves of eucommia ulmoides.200212774353
primary structural determination of n-terminally blocked peptides from the bark of eucommia ulmoides oliv by mass spectrometric analysis.sequencing of n-terminally blocked proteins/peptides is a challenge since these molecules inhibit processing by edman degradation. by using high accuracy fourier transform ion cyclotron resonance (fticr) tandem mass spectrometry and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tofms), the primary structures of two novel n-terminally blocked antifungal peptides (eafp1 and eafp2), purified from the bark of eucommia ulmoides oliv, have been determined. the res ...200312717762
rapid and simultaneous analysis of some bioactive components in eucommia ulmoides by capillary electrophoresis.a micellar electrokinetic chromatography method was established for the qualitative and quantitative determination of three groups of bioactive components, iridoids, flavonoids and phenolic compounds, in eucommia ulmoides. of the eleven bioactive components being studied, ten were successfully separated in 50 mm boric acid buffer at ph 9.5, with 50 mm sodium dodecylsulfate and 4% 1-butanol, at a voltage of 20 kv, temperature of 20 degrees c and injection under high pressure at 138 kpa for 5 s in ...200312650263
time-course of programmed cell death during leaf senescence in eucommia ulmoides.leaves of eucommia ulmoidesoliv. harvested between april to november were examined for programmed cell death (pcd) during growth and senescence. leaves developed in april, becoming fully expanded in late may, remaining unchanged until november when they started to dehisce. falling leaves retained a green color. our results showed that (1) mesophyll cells gradually reduced their nuclei from september to november, (2) positive tunel signals appeared on the nuclei from august, (3) ladder-like dna f ...200312605294
[studies on flavonoids from leave of eucommia ulmoides oliv].to separate the constituents from eucommia ulmoides.200012512449
eucommia (eucommiaceae), a potential biothermometer for the reconstruction of paleoenvironments.the living trees of eucommia ulmoides, an endemic species in china, grow from 200 to 1700 m above sea level, within the geographic range from 102° e to 118° e and from 25° n to 35° n. spring temperatures in these regions vary from 12.3°c to 20.1°c. a physiological study (using germination tests) of e. ulmoides has been undertaken to test the role of spring temperature as a factor controlling the distribution of eucommia. results show that the spring temperature is a limiting factor for eucommia ...200321659074
in-situ chemical analyses of trans-polyisoprene by histochemical staining and fourier transform infrared microspectroscopy in a rubber-producing plant, eucommia ulmoides oliver.the localization of polyisoprene in young stem tissues of eucommia ulmoides oliver was investigated by histochemical staining and fourier transform infrared (ft-ir) microspectroscopy. the fibrous structures were stained with oil red o. ft-ir microspectroscopic analysis proved that the fibrous structures were trans-polyisoprene. granular structures stained with the dye, and characteristic absorptions at 2,960 cm(-1) and 1,430 cm(-1) in ft-ir suggested that trans-polyisoprene accumulated in the vi ...200212355153
crystallization and preliminary crystallographic studies of a novel antifungal protein with five disulfide bridges from eucommia ulmoides oliver.two antifungal proteins, named eucommia antifungal peptides 1 and 2 (eafp1 and eafp2), have been purified from the bark of the tree e. ulmoides oliver and show a relatively broad spectrum of antifungal activity against several agriculturally important plant pathogens. one of these small proteins (eafp2) has been crystallized. the crystal belongs to space group p2(1), with unit-cell parameters a = 19.01, b = 23.16, c = 30.69 a, beta = 98.54 degrees. 1.0 a resolution data were collected from an ea ...200212351831
[sequence analysis of 25s rdna from chinese medicinal plant duzhong eucommia ulmoides oliv].to provide new molecular data for the identification of the chinese medicinal plant duzhong (eucommia ulmoides).199812242816
the occurrence of geometric polyprenol isomers in the rubber-producing plant, eucommia ulmoides oliver.the chain length and geometric isomerism of polyprenols from eucommia ulmoides oliver were analyzed using supercritical fluid chromatography. after intensive effort to establish separation conditions for geometric isomers, a phenyl-bonded silica gel-packed column was found that cleanly separated poly-trans and -cis prenols. the presence of long-chain poly-trans prenols (>9 mers) was confirmed for the first time in plants. trans isomers were found in the leaf, seed coat, and root, but not in the ...200111521971
[a reversed-phase high performance liquid chromatographic method for the determination of pinoresinol diglucopyranoside in eucommia ulmoides oliv].a reversed-phase high performance liquid chromatographic method for the determination of (+)-pinoresinol-di-o-beta-d-glucopyranoside in the bark of eucommia ulmoides was established. the air-dried bark of e. ulmoides was ground into powder and extracted continuously with methanol. the extract was evaporated under reduced pressure, then suspended in water. this suspension being subjected to column chromatography was eluted successively with water, 30%, 50% and 90% aqueous solution of ethanol. the ...199811326985
antioxidant actions of du-zhong (eucommia ulmoides oliv.) toward oxidative damage in biomolecules.this study aimed to investigate the antioxidant effect of water extracts of du-zhong (wedz) on oxidative damage in biomolecules such as deoxyribose, dna, and 2'-deoxyguanosine (2'-dg) as induced by fenton reaction. the wedz used included leaves, raw cortex, and roasted cortex. all of the wedz inhibited the oxidation of deoxyribose induced by fe(3+)-edta/h2o2/ascorbic acid in a concentration dependent manner. at a concentration of 1.14 mg/ml, the inhibitory effect of the extracts of leaves, roast ...200011210714
reactive oxygen species scavenging activity of du-zhong (eucommia ulmoides oliv.) and its active compounds.the biologically active compounds and free radical-/ or reactive oxygen species (ros)-/ scavenging effect of water extract from du-zhong (wedz) were investigated. the wedz used included leaves, raw cortex, and roasted cortex. the hot water extract of du-zhong leaves showed marked activity as a ros scavenger, and the scavenging effect was concentration dependent. the extract of roasted cortex exhibited a modest scavenging effect on ros, while the extract of raw cortex had the weakest scavenging e ...200010956129
antimutagenicity of tochu tea (an aqueous extract of eucommia ulmoides leaves): 1. the clastogen-suppressing effects of tochu tea in cho cells and mice.the suppressing effect of crude extracts of tochu tea, an aqueous extract of eucommia ulmoides leaves and a popular beverage in japan, on the induction of chromosome aberrations in cho cells and mice was studied. when cho cells were treated with tochu tea crude extract after mmc treatment, the frequency of chromosome aberrations was reduced. out of 17 tochu tea components, 5 irridoids (geniposidic acid, geniposide, asperulosidic acid, deacetyl asperulosidic acid, and asperuloside) and 3 phenols ...19979025787
improvement in raw meat texture of cultured eel by feeding of tochu leaf powder.the muscle of eels fed tochu (eucommia ulmoides oliver) leaf powder was 1.8 times harder than the control. the component analysis showed no difference in moisture, lipid, or protein content between the muscles of the control and the tochu-fed eels. not only the extracted neutral fat but also the compound fat of the raw muscles of the tochu-fed eel and the control had the same tlc patterns and fatty acid composition by gas chromatographic analysis. there was a great difference between the tochu-f ...199327314770
[identification of morphology and inorganic elements of eucommia ulmoides oliv. produced in sichuan by applying scanning electron microscope with energy dispersive x-ray analysis].a research on the identification of morphology and inorganic elements of eucommia ulmoides produced in sichuan has been made by applying scanning electron microscope; energy dispersive x-ray analysis. qualitative and semi-quantitative analyses of the inorganic elements in the observed areas have been obtained.19921418547
[a study of commonly used substitutes for chinese drug cortex eucommiae(duzhong)].we have found that 48 species belonging to 10 families and 17 genera are being used as ethnic substitutes for cortex eucommiae (duzhong). a key to all the species has been made to identify them and to distinguish them from eucommia ulmoides.19892635596
[experimental studies on the significance of processing eucommia ulmoides oliv].the inhibitory action on isolated uterus of middle-pregnancy mice have been compared in eucommia ulmoides and its salt water processed product. it has been found that after processing the inhibitory action on the automatic contraction of isolated uterus, the antagonism on spasmodis contraction caused by acetylcholine are increased, but the antagonism on spasmodis contraction caused by pituitarium posterius and the pb content are decreased.19892619889
[measurement of mefv in 66 cases of asthma in the convalescent stage and after treatment with chinese herbs].this paper reported the measurement of maximal expiratory flow-volume curve (mefv) for 66 cases of asthmatics in the convalescent stage. among which the data of fev, pef, v75, v50, v25 in 35 cases (53.03% of the total) gave different abnormal as compared with healthy persons. it showed that in the convalescent stage, most of the asthmatics still possessed obstruction of airways and chiefly of small airways. 35 cases of asthmatics in the convalescent stage was given the chinese herbal decoction o ...19892611953
[comparison of some trace element contents of eucommia ulmoides oliv. and its processed products].a comparison of the contents of 7 trace elements (zn, mn, cu, fe, ca, p, pb) in eucommia ulmoides and its processed products has been made, and the significance of processing eucommia ulmoides is discussed in light of the experimental results.19892597322
anticomplementary activity of the constituents of eucommia ulmoides bark.a study was made on the anticomplementary activity of the lignoid and iridoid derivatives isolated from the bark of eucommia ulmoides as well as the enzymatic hydrolysis products of the isolated iridoid glucosides.20143193787
[treatment of patients with hypertension with an extract of the leaves of eucommia ulmoides]. 19836868493
regeneration of re-covered bark in eucommia ulmoides.the girdled trunk of eucommia ulmoides, sometimes could not regenerate a new bark due to ringed surface necrosis. but, if an extension of 10cm of the bark on each end of the necrotic part was removed in time and re-covered with new bark taken from another tree, the re-covered bark and the newly girdled part could heal together one week after. opposite to the necrotic surface the loose part of the re-covered bark regenerated callus from the phloem ray cells. if the re-covered part was very close ...19836867680
pharmacological action of eucommia ulmoides, oliv. 195713480752
[guttapercha content of the leaves of eucommia ulmoides oliv]. 200613305607
[chemical composition of eucommia ulmoides oliv. leaves]. 195214906238
[treatment of hypertension with eucommia ulmoides]. 195114809468
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