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facilitated establishment of quercus ilex in shrub-dominated communities within a mediterranean ecosystem: do mycorrhizal partners matter?positive plant-plant interaction is a widespread phenomenon, especially in harsh environments, which can contribute to secondary successions. here, we investigated whether arbutus unedo positively influences quercus ilex establishment in shrub communities by abiotic and/or biotic interactions in a mediterranean forest ecosystem, where we previously showed that a. unedo and q. ilex share numerous species of mycorrhizal fungi. in a first field experiment, patterns of q. ilex survivorship were docu ...200919175678
in situ investigation of leaf water status by portable unilateral nuclear magnetic resonance.a portable unilateral nuclear magnetic resonance (nmr) instrument was used to detect in field conditions the water status of leaves of herbaceous crops (zea mays, phaseolus vulgaris), mesophyllous trees (populus nigra), and natural mediterranean vegetation characterized by water-spending shrubs (cistus incanus) and water-saving sclerophyllous trees (quercus ilex). a good relationship was observed between nmr signal, leaf relative water content, and leaf transpiration in herbaceous leaves undergo ...200919193862
comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using dpph, abts, frap, sod, and orac assays.aqueous extracts of 30 plants were investigated for their antioxidant properties using dpph and abts radical scavenging capacity assay, oxygen radical absorbance capacity (orac) assay, superoxide dismutase (sod) assay, and ferric reducing antioxidant potential (frap) assay. total phenolic content was also determined by the folin-ciocalteu method. antioxidant properties and total phenolic content differed significantly among selected plants. it was found that oak (quercus robur), pine (pinus mari ...200919199445
plasticity and stress tolerance override local adaptation in the responses of mediterranean holm oak seedlings to drought and cold.plant populations of widely distributed species experience a broad range of environmental conditions that can be faced by phenotypic plasticity or ecotypic differentiation and local adaptation. the strategy chosen will determine a population's ability to respond to climate change. to explore this, we grew quercus ilex (l.) seedlings from acorns collected at six selected populations from climatically contrasting localities and evaluated their response to drought and late season cold events. maxim ...200919203935
modelling the drought impact on monoterpene fluxes from an evergreen mediterranean forest canopy.in many ecosystems drought cycles are common during the growing season but their impact on volatile monoterpene emissions is unclear. therefore, we aimed to develop and evaluate a process-based modelling approach to explore the explanatory power of likely mechanisms. the biochemically based isoprene and monoterpene emission model sim-bim2 has been modified and linked to a canopy model and a soil water balance model. simulations are carried out for quercus ilex forest sites and results are compar ...200919219456
effects of mate tea (ilex paraguariensis) ingestion on mrna expression of antioxidant enzymes, lipid peroxidation, and total antioxidant status in healthy young women.the antioxidant activity of mate tea, the roasted product derived from yerba mate (ilex paraguarienis), was observed in vitro and in animal models, but studies in humans are lacking. the aim of this study was to investigate the effects of mate tea supplementation on plasma susceptibility to oxidation and on antioxidant enzyme gene expression in healthy nonsmoking women, after acute or prolonged ingestion. we evaluated plasma total antioxidant status (tas), the kinetics of diene conjugate generat ...200919219987
differential and interactive effects of temperature and photoperiod on budburst and carbon reserves in two co-occurring mediterranean oaks.effects of temperature and photoperiod and their interactions on budburst and on the use of carbon reserves were examined in two mediterranean oaks differing in wood anatomy and leaf habit. seedlings of quercus ilex subsp. ballota (evergreen and diffuse-porous wood) and q. faginea (semi-deciduous and ring-porous wood) were grown under two temperatures (12 and 19 degrees c) and two photoperiods (10 and 16 h) in a factorial experiment. in the 16 h photoperiod at 19 degrees c, photosynthesis was su ...200919228321
contrasting nuclear and cytoplasmic exchanges between phylogenetically distant oak species (quercus suber l. and q. ilex l.) in southern france: inferring crosses and dynamics.gene flow is particularly frequent in the genus quercus (oaks), especially between closely related species. we focus here on quercus ilex and the cork-producing quercus suber, which occasionally hybridize although they are phylogenetically markedly separated. morphological observations were combined with both allozymic and chloroplastic diagnostic markers to characterize hybridization and introgression and to infer their dynamics in two french regions (french catalonia and provence), which are s ...200919228328
detection of hybrids in nature: application to oaks (quercus suber and q. ilex).powerful and accurate detection of first-generation (f1) hybrids and backcrosses in nature is needed to achieve a better understanding of the function and dynamics of introgression. to document the frequency of ongoing interspecific gene exchange between two mediterranean evergreen oaks, the cork oak (quercus suber) and the holm oak (q. ilex), we analyzed 1487 individuals originating from across the range of the two species using eight microsatellite loci and two bayesian clustering approaches ( ...200919240752
chlorogenic acid protects paraoxonase 1 activity in high density lipoprotein from inactivation caused by physiological concentrations of hypochlorite.we hypothesized that chlorogenic acids, the main phenolics in coffee, many fruits and ilex paraguariensis extracts, protect paraoxonase 1 activity in hdl from inactivation by chlorination at concentrations of hocl (50 microm) and chlorogenic acid (2-10 microm) compatible with those found in humans. when human hdl was incubated in the presence of hocl/ocl-, a concentration dependent loss of activity was apparent. of interest, 5 caffeoylquinic acid at 5 micromol/l affords more than 60% protection ...200919248222
brazilian natural medicines. iii. structures of triterpene oligoglycosides and lipase inhibitors from mate, leaves of ilex paraguariensis.the methanolic extract from the leaves of ilex paraguariensis (aquifoliaceae) was found to show an inhibitory activity on porcine pancreatic lipase. from the methanolic extract, three new triterpene oligoglycosides, mateglycosides a, b, and c, were isolated together with 18 known compounds. the chemical structures of new oligoglycosides were elucidated on the basis of chemical and physicochemical evidence. several constituents showed inhibitory activities on pancreatic lipase.200919252316
meloidogyne incognita and m. arenaria reproduction on dwarf hollies and lantana.meloidogyne incognita and m. arenaria reproduction and host plant tolerance were assessed in field and greenhouse experiments on seven holly cultivars including ilex glabra 'shamrock', i. vomitoria 'schelling's dwarf', i. cornuta 'carissa', red holly hybrid (ilex little red), and i. crenata 'compacta', 'green luster', and 'helleri' as well as japanese boxwood (buxus microphylla) and two lantana cultivars (lantana camara 'miss huff' and 'new gold'). boxwood had the highest m. arenaria and m. inco ...200119265898
a novel flavanone from ilex hainanensis merr.chemical investigation of the leaves of ilex hainanensis merr. yielded a new flavanone named huazhongilexone-7-o-beta-d-glucopyranoside, together with five known compounds, which included caffeic acid, quercetin, isoquercitrin, quercitrin-7-o-beta-d-glucopyranoside and rutin. all of the compounds were isolated from the plant for the first time. the structure of the new compound was determined on the basis of high resolution mass, 1d and 2d nmr spectroscopies including roesy. the antioxidant acti ...200919296387
importance of mesophyll diffusion conductance in estimation of plant photosynthesis in the field.mesophyll diffusion conductance to co(2) (g(m)) is an important leaf characteristic determining the drawdown of co(2) from substomatal cavities (c(i)) to chloroplasts (c(c)). finite g(m) results in modifications in the shape of the net assimilation (a) versus c(i) response curves, with the final outcome of reduced maximal carboxylase activity of rubisco (v(cmax)), and a greater ratio of the capacity for photosynthetic electron transport to v(cmax) (j(max)/v(cmax)) and alterations in mitochondria ...200919305021
japanese hollies: intolerant hosts of meloidogyne arenaria in microplots.japanese hollies were itttolerant of meloidogyne arenaria in field microplot experiments. ilex crenata var. rotundifolia was relatively more tolerant than i. crenata var. convexa or i. crenata var. helleri. when m. arenaria was added at various itfitial population densities to soil containing plants of "helleri," "convexa," and "rotundifolia," respectively, 91, 75, and 25% were killed by the end of the third growing season. no control plants died during the same period. initial numbers of m. are ...197719305618
susceptibility of japanese holly to criconemoides xenoplax, tylenchorhynchus claytoni, and certain other plant-parasitic nematodes.three cultivars of ilex crenata: 'helleri', 'convexa', and 'rotundifolia' were inoculated with either criconemoides xenoplax, helicotylenchus dihystera, hoplolaimus galeatus, trichodorus cltristiei, or tylenchorhynchus claytoni at 0, 200 (low), or 2,000 (high) nematodes/ 15-cm diam pot. plants were kept in the greenhouse 10 mo prior to transplanting into 2.25 m(2) field plots. helleri was severely stunted by c. xenoplax. criconemoides xenoplax and t. claytoni caused lower plant vigor and top wei ...197619308191
[effect of yiqi wenyang huoxue recipe on bone marrow stem cells' mobilization and its influence on heart function in patients with myocardial infarction].to observe the effect of yiqi wenyang huoxue recipe (ywhr) on bone marrow stem cells' mobilization and heart function of myocardial infarction (mi) patients.200819317161
improving abnormal hemorheological parameters in apoe-/- mice by ilex kudingcha total saponins.the aim of this study was to test the effects of ilex kudingcha total saponins on hemorheology of apoe-/- mice suffering from hypercholesterolemia induced by high-cholesterol diet. the mice were randomly divided into six groups: the control group, the high-cholesterol diet group, 50 mg/kg atorvastatin treatment group, 75, 150 and 300 mg/kg ilex kudingcha saponins treatment groups, and all the drug treatment groups were fed with a high-cholesterol diet. after administration with saponins (150 mg/ ...200919363238
cadmium availability in soil and retention in oak roots: potential for phytostabilization.afforestation of contaminated land by trees is considered as a feasible strategy for the extensive stabilization of contaminants. in this work, we studied the patterns of metal availability (cd, cu, pb and zn) in a contaminated and afforested area. specifically, we observed the response of holm oak (quercus ilex subsp. ballota) leaves to changes in the availability of metals under field conditions, focusing on cd. under controlled conditions we studied the performance of oak seedlings exposed to ...200919375778
caffeic and chlorogenic acids in ilex paraguariensis extracts are the main inhibitors of age generation by methylglyoxal in model proteins.the present study concentrates on the evaluation of the anti-glycation effect of some bioactive substances present in yerba maté (ilex paraguariensis): 5-caffeoylquinic acid, caffeic acid and a sapogenin (oleanolic acid). bovine serum albumin and histones were incubated in the presence of methylglyoxal with or without the addition of 5-caffeoylquinic acid, caffeic acid and oleanolic acid. after the incubation period, advanced glycation end product (age) fluorescence spectra were performed and pr ...200919409454
triterpenoid glycosides from the leaves of ilex pernyi.ten triterpenoid glycosides, including five new ones (1-5), were isolated from the leaves of ilex pernyi. the chemical structures of 1-5 were determined on the basis of the chemical and spectroscopic evidence.200919420788
a method to quantify transesterification activities of lipases in litters.lipases are glycerol ester hydrolases (ec 3.1.1.3) produced by a wide range of microorganisms. they catalyse the hydrolysis of different esters but this reaction is reversible, depending on the water content of the reaction medium, via esterification and transesterification. the synthetic activity of lipases can be of major importance in natural ecosystems since it can be involved in carbon stockage in soils or litters. here, the detection of transesterification activities of lipases in litter i ...200919426767
antiobesity effects of yerba maté extract (ilex paraguariensis) in high-fat diet-induced obese mice.because the potential of yerba maté (ilex paraguariensis) has been suggested in the management of obesity, the aim of the present study was to evaluate the effects of yerba maté extract on weight loss, obesity-related biochemical parameters, and the regulation of adipose tissue gene expression in high-fat diet-induced obesity in mice. thirty animals were randomly assigned to three groups. the mice were introduced to standard or high-fat diets. after 12 weeks on a high-fat diet, mice were randoml ...200919444227
inhibitory effect of triterpenoid saponins from the leaves of ilex kudingcha on aggregated ldl-induced lipid deposition in macrophages.we have investigated the inhibitory effect of triterpenoid saponins from the leaves of ilex kudingcha c. j. tseng on aggregated low-density lipoprotein (ldl)-induced lipid deposition in macrophages. a cell-based screening model was initially applied on aggregated ldl (aggldl)-induced lipid deposition on macrophages to test the inhibitory effects of the 12 triterpenoid saponins from this plant. eight of these compounds inhibited the formation of foam cells and reduced intracellular total choleste ...200919468972
maté tea inhibits in vitro pancreatic lipase activity and has hypolipidemic effect on high-fat diet-induced obese mice.the inhibitory effects of maté tea (mt), a beverage produced with leaves from ilex paraguariensis, in vitro lipase activity and on obesity in obese mice models were examined. for the in vitro experiment, porcine and human pancreatic lipase (pl) activities were determined by measuring the rate of release of oleic acid from hydrolysis of olive oil emulsified with taurocholate, phospholipids, gum arabic, or polyvinyl alcohol. for the in vivo experiments, animals were fed with a standard diet (sd, n ...201019543216
acorn crop size and pre-dispersal predation determine inter-specific differences in the recruitment of co-occurring oaks.the contribution of pre-dispersal seed predation to inter-specific differences in recruitment remains elusive. in species with no resistance mechanisms, differences in pre-dispersal predation may arise from differences in seed abundance (plant satiation) or in the ability of seeds to survive insect infestation (seed satiation). this study aimed to analyse the impact of pre-dispersal acorn predation by weevils in two co-occurring mediterranean oaks (quercus ilex and quercus humilis) and to compar ...200919544074
ancient and current gene flow between two distantly related mediterranean oak species, quercus suber and q. ilex.quercus suber and q. ilex are distantly related and their distributions partially overlap. they hybridize occasionally, but the complete replacement of q. suber chloroplast dna (cpdna) by that of q. ilex was identified in two specific geographical areas. the objective of this study was to determine whether the contrasting situation reflected current or recent geographical interspecies gene flow variation or was the result of ancient introgression.200919556263
seasonal carbon storage and growth in mediterranean tree seedlings under different water conditions.in all mediterranean-type ecosystems, evergreen and deciduous trees differing in wood anatomy, growth pattern and leaf habit coexist, suggesting distinct adaptative responses to environmental constraints. this study examined the effects of summer water stress on carbon (c) storage and growth in seedlings of three coexisting mediterranean trees that differed in phenology and wood anatomy characteristics: quercus ilex subsp. ballota (desf.) samp., quercus faginea lam. and pinus halepensis l. seedl ...200919574258
in vitro anticancer potential of tree extracts from the walloon region forest.forty-eight extracts from 16 common belgian trees from the walloon region forest were evaluated for in vitro growth inhibitory activity against the human lovo colon cancer, pc3 prostate cancer, and u373 glioblastoma cell lines. our study was performed with the aim of selecting plant candidates in order to later isolate new anticancer compounds from an easily affordable tree material. extracts from alnus glutinosa (stem bark), carpinus betulus (leaves and stem bark), castanea sativa (stem bark), ...200919579185
comparison of major phenolic constituents and in vitro antioxidant activity of diverse kudingcha genotypes from ilex kudingcha, ilex cornuta, and ligustrum robustum.a total of seven kudingcha genotypes from three plant species (ilex kudingcha, ilex cornuta, and ligustrum robustum) with different geographic origins in china were investigated for their major phenolic compounds, individual and total phenolics contents, and in vitro antioxidant properties (abts, dpph, frap, and oh assays). lc-pda-apci-ms analysis showed that kudingcha genotypes from ilex and ligustrum had entirely different phenolic profiles. major phenolics in kudingcha genotypes from two ilex ...200919601659
[triterpenoids from leaves of ilex cornuta].to investigate the chemical constituents of ilex cornuta.200919639785
consumption of yerba mate ( ilex paraguariensis ) improves serum lipid parameters in healthy dyslipidemic subjects and provides an additional ldl-cholesterol reduction in individuals on statin therapy.the objective of this study was to verify the effect of yerba mate (ilex paraguariensis) consumption on lipid and lipoprotein levels in humans. one hundred and two individuals participated of this single-blind controlled trial. normolipidemic (n = 15), dyslipidemic (n = 57), and hypercholesterolemic subjects on long-term statin therapy (n = 30) ingested 330 ml, 3 times/day, of green or roasted yerba mate infusions for 40 days. in normolipidemic subjects, yerba mate consumption reduced ldl-choles ...200919694438
study of the antifungal activity of bacillus vallismortis zz185 in vitro and identification of its antifungal components.an endophytic bacillus vallismortis zz185 was isolated from healthy stems of the plant broadleaf holly (ilex latifolia thunb) collected in nanjing, china. both the culture filtrate and the n-butanol extract of strain zz185 showed strong growth inhibition activity in vitro against the phytopathogens fusarium graminearum, alternaria alternata, rhizoctonia solani, cryphonectria parasitica and phytophthora capsici. the results showed that the filtrate and extract reduced the symptoms of wheat seedli ...201019717300
high efficiency liquid chromatography techniques coupled to mass spectrometry for the characterization of mate extracts.there is growing interest related to rapid screening and full characterization of the constituents of plants with medicinal properties; among these, "mate" or yerba maté is a tea-like beverage widely consumed in south america, obtained from the dried leaves of ilex paraguariensis. the high content in polyphenols accounts for in vitro and in vivo antioxidant activity of the extracts obtained from this plant; on the other hand, the high complexity of the samples extracted, depending on the method ...200919726042
measuring the postfire resilience of a bird-vegetation system: a 28-year study in a mediterranean oak woodland.despite numerous studies on the response of mediterranean ecosystems to fire, few have measured the respective resilience of vegetation and fauna compartments. for 28 years, we conducted an annual monitoring of avifauna composition and vegetation structure (cover profile)following a severe wildfire in a holm oak (quercus ilex) stand in southern france. our aim was to estimate the time necessary for this bird-vegetation system to return to a state analogous to its pre-fire state. in the burned pl ...200919727831
evaluation of the cyto- and genotoxic activity of yerba mate (ilex paraguariensis) in human lymphocytes in vitro.despite its antioxidant capacity and well-known health benefits, yerba mate tea (ilex paraguariensis) has been shown to possess some genotoxic and mutagenic activities and to increase incidence of some types of cancer. the aim of this study was to estimate the cyto- and genotoxicity of mate tea in human peripheral lymphocytes in vitro. we found that yerba mate extract induced a concentration-dependent, statistically significant increase in the level of apoptotic and necrotic cells and a decrease ...200919733686
chemical constituents from the leaves of ilex paraguariensis inhibit human neutrophil elastase.human neutrophil elastase (hne), a serine protease with broad target specificity, is the only enzyme responsible for the degradation of elastin which is an insoluble elastic fibrous protein in animal connective tissue. biologically, elastase activity significantly increased with age, which results in a reduced skin elasticity and in the appearance of wrinkles or stretchmarks. in the course of our screening program for hne inhibitors from natural source, the meoh extract of ilex paraguariensis le ...200919784576
saponins in yerba mate tea ( ilex paraguariensis a. st.-hil) and quercetin synergistically inhibit inos and cox-2 in lipopolysaccharide-induced macrophages through nfkappab pathways.yerba mate tea ( ilex paraguariensis ) is growing in popularity around the world. the objective of this study was to investigate the potential anti-inflammatory effect of yerba mate tea (mt) extracts as well as some of its phytochemicals and their interactions. mt and decaffeinated mt extracts [1-300 microm chlorogenic acid (cha) equiv]; cha, caffeine from mt (matein), and mate saponins (1-300 microm); quercetin (1-200 microm); and ursolic and oleanolic acids (1-100 microm) were tested by measur ...200919807157
antifungal activity of the aqueous extract of ilex paraguariensis against malassezia furfur.malassezia furfur is a lipodependent, dimorphic and saprophyte fungus which causes pityriasis versicolor, dandruff and seborrheic dermatitis in humans. the drugs available to treat this fungal infection are few. these drugs are highly toxic and are costly when used in prolonged treatments. for these reasons, it is necessary to find new compounds to treat these infections. ilex paraguariensis st hilaire is a plant that grows in argentina, brazil and paraguay. the aim of this study was to evaluate ...201019827026
platinum and rhodium associated with the leaves of nerium oleander l.; analytical method using voltammetry; assessment of air quality in the palermo (italy) area.a rapid accumulation of the catalytic active noble metals in the environmental and biological matrices was observed and concern arose about potential environmental and health risks. the development of reliable analytic methods to measure very low pt and rh concentrations is required. the main purpose of this work was to develop a reliable method for the determination of pt and rh in environmental matrices because of inherent difficulties in using conventional techniques used, in particular, the ...201019846253
characterization of the yerba mate (ilex paraguariensis) volatile fraction using solid-phase microextraction-comprehensive 2-d gc-ms.the present research is focused on the use of a solid-phase microextraction-comprehensive 2-d gc methodology, in the analysis of the volatile fraction of yerba mate. yerba mate is used for the generation of a tea-like beverage, widely consumed in south america. a rapid-scanning quadrupole mass spectrometer (qms), employed as a detection system and operated at a 25 hz scanning frequency, supplied high-quality mass spectra. the effectiveness of the 3-d comprehensive 2-d gc-qms experiment was compa ...200919877141
analysis of phenolic glycosides from ilex litseaefolia using electrospray ionization quadrupole time-of-flight mass spectrometry. 200919902421
[mercury pollution investigation in predominant plants surrounding shenzhen qingshuihe municipal solid waste incineration plant].in order to investigate the effects of mercury emission from municipal solid waste incineration (mswi) on the surrounding plants and soils, the mercury concentrations were examined in the plant samples including leaves and stems and the soil samples around shenzhen qingshuihe mswi plant. results show that, these plants are significantly polluted by mercury, the mercury concentrations of the plant leaves are 0.030 9-0.246 7 mg x kg(-1), with the mean value 0.094 8 mg x kg(-1), among the local pro ...200919927841
chemical kinetics of 5-o-caffeoylquinic acid in superheated steam: effect of isomerization on mate (ilex paraguariensis) manufacturing.a set of experiments was carried out to investigate the chemical stability of 5-o-caffeoylquinic acid (5-cqa) in the presence of superheated steam. a batch cylindrical reactor made of glass and isothermally operated between 398 and 499 k was used in the experiments. a high-performance liquid chromatograph equipped with a diode array detector was applied to monitor the 5-cqa concentrations. the conversions of 5-cqa were correctly reproduced with a simplified kinetic model represented by a reversi ...200919938862
[first record of zoophthora radicans (entomophthorales: entomophthoraceae) on adults of the paraguay tea psyllid, gyropsylla spegazziniana lizer & trelles (hemiptera: psyllidae), in brazil].we report the first occurrence of zoophthora radicans infecting adults of gyropsylla spegazziniana lizer & trelles in a commercial paraguay tea plantation (ilex paraguariensis), in cascavel, pr, brazil. the fungus prevalence was high (90% of mortality), considered a natural epizooty.200919943025
ring b aromatic steroids from an endophytic fungus, colletotrichum sp.the new (22e,24r)-3-acetoxy-19(10-->6)-abeo-ergosta-5,7,9,22-tetraen-3beta-ol (1) and the known (22e,24r)-19(10-->6)-abeo-ergosta-5,7,9,22-tetraen-3beta-ol (2), two interesting ergosteroids with rare aromatized ring b, together with seven known derivatives, namely (22e,24r)-ergosta-5,7,22-trien-3beta-ol (3), (22e,24r)-ergosta-4,7,22-trien-3-one (4), (22e,24r)-ergosta-4,6,8(14),22-tetraen-3-one (5), (22e,24r)-5alpha,8alpha-epidioxyergosta-6,22-dien-3beta-ol (6), (22e,24r)-ergosta-7,22-dien-3beta, ...200919967971
are pine plantations valid tools for restoring mediterranean forests? an assessment along abiotic and biotic gradients.the ecological impacts of forest plantations are a focus of intense debate, from studies that consider plantations as "biological deserts" to studies showing positive effects on plant diversity and dynamics. this lack of consensus might be influenced by the scarcity of studies that examine how the ecological characteristics of plantations vary along abiotic and biotic gradients. here we conducted a large-scale assessment of plant regeneration and diversity in plantations of southern spain. tree ...200920014583
remobilization of acorn nitrogen for seedling growth in holm oak (quercus ilex), cultivated with contrasting nutrient availability.the relative contribution of nitrogen (n) reserves from seeds or uptake by the roots to the growth and n content of young seedlings has received little attention. in this study, we investigated the contribution of n from the acorn or uptake by the roots to the n content of holm oak (quercus ilex l.) seedlings and determined if remobilization of acorn n was affected by nutrient availability in the growing media. q. ilex seedlings were cultivated for 3 months, until the end of the second shoot flu ...201020022863
can npk fertilizers enhance seedling growth and mycorrhizal status of tuber melanosporum-inoculated quercus ilex seedlings?although successful cultivation of the black truffle (tuber melanosporum) has inspired the establishment of widespread truffle orchards in agricultural lands throughout the world, there are many unknowns involved in proper management of orchards during the 6-10 years prior to truffle production, and there are conflicting results reported for fertilizer treatments. here, we systematically evaluate the combined effects of nitrogen, phosphorous, and potassium with different doses of each element, a ...201020033737
do photosynthetic limitations of evergreen quercus ilex leaves change with long-term increased drought severity?seasonal drought can severely impact leaf photosynthetic capacity. this is particularly important for mediterranean forests, where precipitation is expected to decrease as a consequence of climate change. impacts of increased drought on the photosynthetic capacity of the evergreen quercus ilex were studied for two years in a mature forest submitted to long-term throughfall exclusion. gas exchange and chlorophyll fluorescence were measured on two successive leaf cohorts in a control and a dry plo ...201020051039
methylxanthines and phenolics content extracted during the consumption of mate (ilex paraguariensis st. hil) beverages."chimarrao" and "terere" are popular beverages consumed in south america prepared using mate (ilex paraguariensis st. hil.). "chimarrao" consists of a partial infusion where hot water is added, while "terere" is a total infusion, with addition of cold water. this study was designed to simulate preparation of these beverages for consumption, in order to estimate the total amount of xanthines and phenolic compounds in aqueous extracts that would be ingested by the consumer. different commercial ty ...201020058928
suitability of two types of organic wastes for the growth of sclerophyllous shrubs on limestone debris: a mesocosm trial.this study investigated whether overlaying organic wastes directly on limestone debris allowed the growth of sclerophyllous shrubs; the aim was to explore the feasibility of rehabilitation of sites destroyed by quarrying activity. in an open air mesocosm experiment two types of organic material were compared: compost from municipal wastes (c) and a mixture of compost and poultry manure added with wheat husk (c-pw). mesocosms were pots (1m diameter, 60cm height) containing limestone debris covere ...201020092872
transfer of 238u, 230th, 226ra, and 210pb from soils to tree and shrub species in a mediterranean area.the soil-to-plant transfer factors of natural uranium isotopes ((238)u and (234)u), (230)th, (226)ra, and (210)pb were studied in a disused uranium mine located in the extremadura region in the south-west of spain. the plant samples included trees (quercus ilex, quercus suber, and eucalyptus cameldulensis) and one shrub (cytisus multiflorus). all of them are characteristic of mediterranean environments. the activity concentrations in leaves and fruit were determined for the tree species at diffe ...201020153657
quantification of saponins in extractive solution of mate leaves (ilex paraguariensis a. st. hil.).mate (ilex paraguariensis a. st. hil.) is a raw material used to prepare popular beverages in south america, whose healthy effects have been pointed out. the leaves and stems of mate contain a complex assemblage of saponins, mainly ursolic acid and oleanolic acid glycosides, and their quantification presents difficulties such as weak cromophorous sites and interference of other compounds. on the other hand, the sugar moieties are a chief character that distinguishes them. we developed and valida ...201020170360
triterpene saponins from the leaves of ilex kudingcha.three new triterpene saponins, ilekudinosides t-v (1-3), along with six known saponins were isolated from the 70% ethanolic extract of the leaves of ilex kudingcha. the new saponins were characterized as 3-o-beta-d-glucopyranosyl-(1 --> 3)-[alpha-l-rhamnopyranosyl-(1 --> 2)]-alpha-l-arabinopyranosyl-3beta,19alpha-dihydroxy-urs-12(13)-en-28,20beta-lactone (1), 3-o-beta-d-glucopyranosyl-(1 --> 3)-[alpha-l-rhamnopyranosyl-(1 --> 2)]-alpha-l-arabinopyranosyl-3beta,19alpha-dihydroxy-12-ethoxy-urs-13( ...200920183290
effects of forest fragmentation on seed dispersal and seedling establishment in ornithochorous trees.habitat fragmentation increases seed dispersal limitation across the landscape and may also affect subsequent demographic stages such as seedling establishment. thus, the development of adequate plans for forest restoration requires an understanding of mechanisms by which fragmentation hampers seed delivery to deforested areas and knowledge of how fragmentation affects the relationship between seed-deposition patterns and seedling establishment. we evaluated the dispersal and recruitment of two ...201020184646
phenolic compounds, antioxidant activity and in vitro inhibitory potential against key enzymes relevant for hyperglycemia and hypertension of commonly used medicinal plants, herbs and spices in latin america.traditionally used medicinal plants, herbs and spices in latin america were investigated to determine their phenolic profiles, antioxidant activity and in vitro inhibitory potential against key enzymes relevant for hyperglycemia and hypertension. high phenolic and antioxidant activity-containing medicinal plants and spices such as chancapiedra (phyllantus niruri l.), zarzaparrilla (smilax officinalis), yerba mate (ilex paraguayensis st-hil), and huacatay (tagetes minuta) had the highest anti-hyp ...201020185303
metabolic classification of south american ilex species by nmr-based metabolomics.the genus ilex to which mate (ilex paraguariensis) belongs, consists of more than 500 species. a wide range of metabolites including saponins and phenylpropanoids has been reported from ilex species. however, despite the previous works on the ilex metabolites, the metabolic similarities between species which can be used for chemotaxonomy of the species are not clear yet. in this study, nuclear magnetic resonance (nmr) spectroscopy-based metabolomics was applied to the classification of 11 south ...201020199787
genomic screening in dioecious "yerba mate" tree (ilex paraguariensis a. st. hill., aquifoliaceae) through representational difference analysis.the "yerba mate" tree, ilex paraguariensis, is a functionally dioecious crop species with economic relevance in several south american countries. we report a genomic screening accomplished through representational difference analysis (rda) in male and female i. paraguariensis trees. the aim of the present paper was to investigate the occurrence of sex-related genomic differences in order to develop an early gender detection molecular method that could help reducing energy inputs during the "yerb ...201020221672
occurrence of polycyclic aromatic hydrocarbons throughout the processing stages of erva-mate (ilex paraguariensis).the presence of polycyclic aromatic hydrocarbons (pahs) in mate (ilex paraguariensis) is believed to be due to the degradation of mate compounds and the burning of wood during the "sapeco" (rapid drying process) and the final drying steps, which are the most important processing stages in mate production. due to the high toxicity of these compounds, studies on their presence in mate are extremely important. the aim of this study was to evaluate pah levels in mate throughout the processing stages ...201020349373
two acetylated megastigmane glycosides from the leaves of ilex paraguariensis.two acetylated megastigmane glycosides, matenosides a (1) and b (2), have been isolated from the meoh extract of ilex paraguariensis leaves, and their structures were elucidated on the basis of spectroscopic analysis. compounds 1 and 2 exhibited human neutrophil elastase (hne) inhibitory activity with ic(50) values of 50.4 mum and 11.1 microm, respectively.201020361300
profiling and characterization by lc-msn of the chlorogenic acids and hydroxycinnamoylshikimate esters in maté (ilex paraguariensis).the chlorogenic acids of mate (ilex paraguariensis) have been investigated qualitatively by lc-ms(n). forty-two chlorogenic acids were detected and all characterized to regioisomeric level on the basis of their fragmentation pattern in tandem ms spectra, 24 of them for the first time from this source. both chlorogenic acids based on trans- and cis-cinnamic acid substituents were identified. assignment to the level of individual regioisomers was possible for eight caffeoylquinic acids (1-8), five ...201020405920
changes in leaf phenology of three european oak species in response to experimental climate change.*because the phenology of trees is strongly driven by environmental factors such as temperature, climate change has already altered the vegetative and reproductive phenology of many species, especially in the temperate zone. here, we aimed to determine whether projected levels of warming for the upcoming decades will lead to linear changes in the phenology of trees or to more complex responses. *we report the results of a 3-yr common garden experiment designed to study the phenological response ...201020406403
new triterpene saponins from the root of ilex pubescens.two new triterpene glycosides named ilexpublesnin a (1) and ilexpublesnin b (2) were isolated from the root of ilex pubescens. their structures were determined as 3-o-(β-d-xylopyranosyl)-28-o-(β-d-glucopyranosyl)-3β, 19α-dihydroxyurs-23-oxo-12-en-28-oic acid (1) and 28-o-(β-d-xylopyranosyl-(2→1)-β-d-glucopyranosyl)-3β, 19α-dihydroxyurs-23-oxo-12-en-28-oic acid (2) on the basis of chemical and spectroscopic methods.201020470872
analysis of free-radical scavenging of yerba mate (ilex paraguriensis) using electron spin resonance and radical-induced dna damage.mate (mt) is a popular south american beverage that has been used as a traditional medicine for centuries, spurring recent interest in its nutraceutical properties. mt is prepared as an infusion of leaves from the yerba mate (llex paraguriensis) tree. mt has been reported to have antioxidant properties in vitro and in vivo, but these have not been fully characterized in terms of effects against specific radicals. accordingly, we examined the antioxidant effects of an mt infusion against hydroxyl ...201020492144
phenolic acids and methylxanthines composition and antioxidant properties of mate (ilex paraguariensis) residue.ilex paraguariensis is known to contain compounds with antioxidant properties, such as phenolic acids, and its stimulant properties are attributed to methylxanthines, such as caffeine. the aims of this study were to evaluate the phenolic, methylxanthinic, and tannin composition of a mate residue (mate powder), to compare the quali-quantitative phenolic composition and the antioxidant potential of extracts obtained from distinct solvent systems. among the extracts prepared with different solvents ...201020492280
changes in monoterpene emission rates of quercus ilex infested by aphids tended by native or invasive lasius ant species.the emission of volatile organic compounds (vocs) depends on temperature and light. other factors such as insect herbivory also may modify voc emission. in particular, aphid feeding promotes the release of new compounds and changes the composition of plant volatile blends. given that some aphids are tended by ants, we investigated whether ants change the emission of vocs indirectly through attendance on aphids. the effect of lachnus roboris aphids and two different tending ant species on terpene ...201020549328
leaf physiological responses to extreme droughts in mediterranean quercus ilex forest.global climate change is expected to result in more frequent and intense droughts in the mediterranean region. to understand forest response to severe drought, we used a mobile rainfall shelter to examine the impact of spring and autumn rainfall exclusion on stomatal (s(l) ) and non-stomatal (ns(l) ) limitations of photosynthesis in a quercus ilex ecosystem. spring rainfall exclusion, carried out during increasing atmospheric demand and leaf development, had a larger impact on photosynthesis tha ...201020561253
drought-induced photosynthetic inhibition and autumn recovery in two mediterranean oak species (quercus ilex and quercus suber).responses of leaf water relations and photosynthesis to summer drought and autumn rewetting were studied in two evergreen mediterranean oak species, quercus ilex spp. rotundifolia and quercus suber. the predawn leaf water potential (ψ(lpd)), stomatal conductance (gs) and photosynthetic rate (a) at ambient conditions were measured seasonally over a 3-year period. we also measured the photosynthetic response to light and to intercellular co₂ (a/ppfd and a/c(i) response curves) under water stress ( ...201020571151
recent advances on ilex paraguariensis research: minireview.ilex paraguariensis dried and minced leaves are made into a brewed tea, prepared in a sui generis manner by large populations in south america, having evolved from a tea drunk by the guarani ethnic group to a beverage that has a social and almost ritualistic role in some south american modern societies. it is used both as a source of caffeine, in lieu or in parallel with tea and coffee, but also as a therapeutic agent for its alleged pharmacological properties. although with some exceptions, res ...201020599603
change in hydraulic traits of mediterranean quercus ilex subjected to long-term throughfall exclusion.mediterranean tree species experience unpredictable climate environments and severe summer droughts and they may be impaired by the trend of decline in precipitation projected as a consequence of global climate change. the response of quercus ilex to drought was studied by measuring hydraulic traits of trees growing in a mature forest subjected to partial throughfall exclusion for 6 years. we measured hydraulic conductivity, xylem vulnerability to embolism, and anatomical features in branches an ...201020621974
genetic consequences of habitat fragmentation in long-lived tree species: the case of the mediterranean holm oak (quercus ilex, l.).large-scale forest fragmentation can increase interpopulation genetic differentiation and erode the genetic variability of remnant plant populations. in this study, we analyze the extent of clonality and the genetic variability and structure within a holm oak (quercus ilex) population from central spain at 3 patches showing different degrees of fragmentation. for this purpose, we have typed 191 individuals (105 adults and 86 saplings) at 9 microsatellite loci. microsatellite markers revealed an ...201020624756
natural hybridisation between kermes (quercus coccifera l.) and holm oaks (q. ilex l.) revealed by microsatellite markers.hybridisation between species of the genus quercus is a common phenomenon as a result of weak reproductive isolation mechanisms between phylogenetically close species that frequently co-occur in mixed stands. in this study, we use microsatellite markers to analyse introgression between kermes (quercus coccifera l.) and holm (q. ilex l.) oak, two closely related taxa that frequently dominate the landscape in extensive areas in the mediterranean region. all tested microsatellites amplified and wer ...201020653907
drinking yerba mate infusion: a potential risk factor for invasive fungal diseases?yerba mate (ilex paraguariensis) infusion is a very popular drink in south america. although several studies have evaluated the potential for fungal contamination in foodstuff, very few investigations have been conducted with yerba mate samples. in order to evaluate for the presence of potentially pathogenic fungi, here we studied 8 brands of yerba mate commercially available in southern brazil. fungal survival in adverse conditions such as gastric ph was determined by incubating samples at ph 1 ...201020659310
evaluation of restoration effectiveness: community response to the removal of alien plants.plant invasions are a key cause of biodiversity loss and motivate many restoration programs worldwide. we assessed restoration success of an invaded forest in the azores using two complementary experimental designs: a before-after control-impact (baci) design compared a restored and a control (unmanipulated) site over three years, while a control-impact (ci) design evaluated the short-term effects of restoration on restored-control replicated pairs. in both designs, a food web approach was used ...201020666243
[effect of ningxintong granule in treating patients of coronary heart disease with left ventricular diastolic dysfunction and qi-deficiency blood-stasis syndrome].to investigate the effect of ningxintong granule (nxtg) on left ventricular diastolic dysfunction (lvdd) in patients with coronary heart disease (chd) of qi-deficiency and blood-stasis syndrome in chinese medicine.201020669668
changes in water content and distribution in quercus ilex leaves during progressive drought assessed by in vivo 1h magnetic resonance imaging.drought is a common stressor in many regions of the world and current climatic global circulation models predict further increases in warming and drought in the coming decades in several of these regions, such as the mediterranean basin. the changes in leaf water content, distribution and dynamics in plant tissues under different soil water availabilities are not well known. in order to fill this gap, in the present report we describe our study withholding the irrigation of the seedlings of quer ...201020735815
platanus pollen allergen, pla a 1: quantification in the atmosphere and influence on a sensitizing population.the allergic response in susceptible patients does not always coincide with the presence and magnitude of airborne pollen counts. the prevalence of allergy to platanus is currently moderate, although the percentage of monosensitized patients is low. this hinders accurate interpretation of the relationship between the amount of pollen inhaled and the patient's symptoms.201020813013
quercus ilex l. carbon sequestration capability related to shrub size.co(2) sequestration capacity of quercus ilex l., an evergreen species developing in shrub and forest communities widely distributed in the mediterranean basin, was analysed. experiments were carried out in the period of january to december 2009 on 20 shrubs of different size, growing at the botanical garden of rome. at shrub level, the largest differences concern total photosynthetic leaf surface area per shrub and shrub volume. shrubs structure significantly contribute to reduce total irradianc ...201020824326
the history of extant ilex species (aquifoliaceae): evidence of hybridization within a miocene radiation.the history and diversification of the genus ilex (aquifoliaceae), based on 108 different species (116 specimens), are inferred from the analysis of two nuclear (its and nepgs) and three plastid (rbcl, trnl-f and atpb-rbcl) sequences. nuclear and plastid trees are highly incongruent and the nuclear tree is more compatible with current taxonomic classifications than the plastid one. the most recent common ancestor (mrca) of extant species is dated from the miocene, although the ilex stem lineage ...201020870023
effects of mediterranean dehesa management on epiphytic lichens.spanish holm oak (quercus ilex subsp. ballota) open woodlands (dehesas) maintain a high diversity of plants and animals compared to other forested mediterranean habits, but little is known about the responses of epiphytic lichens to different management regimes that are applied to this woodland type. the present study was carried out in central-southern spain and included four management regimes: agriculture, grazing of sheep, grassland grazed by wild ungulates (deer), and abandoned dehesas cove ...201020937519
transfer of pesticides to the brew during mate drinking process and their relationship with physicochemical properties.in order to evaluate the extraction of pesticide residues that are transferred to the brew during mate drinking process of p.u.1 yerba mate leaves (ilex paraguariensis), a special device to simulate the way in which mate is drunk in uruguay was developed. the transfer to the brew of 12 organophosphates, 5 synthethic pyrethroids and one organochlorine pesticide from spiked samples was studied. the relationship between the transfer data thus obtained and physicochemical properties like water solub ...201020954047
responses of evergreen and deciduous quercus species to enhanced ozone levels.plants of one evergreen oak (quercus ilex) and three deciduous oaks (q. faginea, with small leaves; q. pyrenaica and q. robur, with large leaves) were exposed both to filtered air and to enhanced ozone levels in open-top chambers. q. faginea and q. pyrenaica were studied for the first time. based on visible injury, gas exchange, chlorophyll content and biomass responses, q. pyrenaica was the most sensitive species, and q. ilex was the most tolerant, followed by q. faginea. functional leaf traits ...201020974507
protective effect of ilex latifolia, a major component of "kudingcha", against transient focal ischemia-induced neuronal damage in rats.ilex latifolia (aquifoliaceae), a primary component of "kudingcha", has been used in chinese folk medicine to treat various kinds of diseases including headaches, inflammatory diseases, and cardiac ischemic injury. the present study investigated the protective effect of the ethanol extract of ilex latifolia against transient, focal, ischemia-induced neuronal damage.201021029769
common plant toxicology: a comparison of national and southwest ohio data trends on plant poisonings in the 21st century.data from the american association of poison control centers (aapcc) and the cincinnati-based drug and poison information center (dpic) were analyzed to determine the incidence and trends of human plant poisonings since the year 2000. approximately 3.4% of the approximately 4.3million annual calls to the aapcc centers involved plants, with a higher fraction (4.5%) for pediatric exposures. nearly 70% of plant exposures occurred in children under six. only 8% of cases required treatment in a healt ...201021034756
mast seeding under increasing drought: results from a long-term data set and from a rainfall exclusion experiment.mast seeding, the synchronous, highly variable seed production among years, is very common in tree species, but there is no consensus about its main causes and the main environmental factors affecting it. in this study, we first analyze a long-term data set on reproductive and vegetative growth of quercus ilex in a mediterranean woodland in order to identify the main environmental drivers of interannual variation in flower and seed production and contrast the impact of climate vs. adaptive facto ...201021058565
[selection of winter plant species for wetlands constructed as sewage treatment systems and evaluation of their wastewater purification potentials].in order to establish an evaluation system for selection of winter wetland plants possessing high wastewater purification potentials in subtropics areas, designed sewage treatment experiments were carried out by introducing into the constructed wetlands 25 species of winter wetland plants. cluster analysis was performed by including harmful environment-resistant enzyme and substrate enzyme activities into the commonly applied plant screening and assessment indexes system. the obtained results in ...201021090294
erinea formation on quercus ilex leaves: anatomical, physiological and chemical responses of leaf trichomes against mite attack.structures on the surfaces of leaves, such as dense layers of non-glandular trichomes, strongly affect phylloplane mite activities. on the other hand the feeding of eriophyoid mites on leaf surfaces can cause hyperplasia of leaf trichomes (erinea formation). in many cases the hyperplasia is accompanied by the accumulation of pigments within trichome cells, causing an impressive red-brown colouration of the erineum. there is no information, however, on the structure of these pigments as well as o ...201021131012
[determination of ilexside ii in leaves of ilex cornuta by hplc-elsd].to develop a hplc-elsd method for determination of ilexside ii in leaves of ilex cornuta.201021137345
tropospheric ozone effects on chemical composition and decomposition rate of quercus ilex l. leaves.we determined the effects of tropospheric ozone on the chemical composition of quercus ilex l. leaves and their decomposition, with a view to assessing the influence of ozone on nutrient cycling and the sustainability of mediterranean holm oak forests. forming one of the most widespread thermophilous vegetation communities in the area, q. ilex is a dominant and widespread evergreen oak in the mediterranean, where concentrations of tropospheric ozone are particularly high. the dynamics of carbon, ...201021167557
wormlike micellar aggregates of saponins from ilex paraguariensis a. st. hil. (mate): a characterisation by cryo-tem, rheology, light scattering and small-angle neutron scattering.this work reports the physico-chemical characterisation of the micellar structures formed by a saponin fraction obtained from an important south american species, ilex paraguariensis (mate). the mate saponin-enriched fraction (msf) mainly comprises triterpenic glycosides and was obtained from mate green fruits through solid-phase extraction. the physico-chemical studies focused on the determination of the critical micellar concentration (cmc), the size and shape of the micelles, using convention ...201021204106
cryopreservation of ilex immature zygotic embryos.tropical ilex species have recalcitrant seeds. this chapter describes protocols for long-term conservation of ilex brasiliensis, i. brevicuspis, i. dumosa, i. microdonta, i. integerrima, i. paraguariensis, i. pseudoboxus, i. taubertiana, and i. theezans through cryopreservation of zygotic rudimentary embryos at the heart developmental stage. the embryos are aseptically removed from the seeds and precultured (7 days) in the dark at 27±2°c on solidified quarter-strength murashige and skoog medium ...201121207272
anti-inflammatory effects of yerba maté extract (ilex paraguariensis) ameliorate insulin resistance in mice with high fat diet-induced obesity.the aim of the present study was to evaluate the effects of yerba maté extract upon markers of insulin resistance and inflammatory markers in mice with high fat diet-induced obesity. the mice were introduced to either standard or high fat diets. after 12 weeks on a high fat diet, mice were randomly assigned to one of the two treatment conditions, water or yerba maté extract at 1.0 gkg(-1). after treatment, glucose blood level and hepatic and soleus muscle insulin response were evaluated. serum l ...201121238540
rhizosphere dynamics during phytoremediation of olive mill wastewater.the potential of phytoremediation as a treatment option for olive mill wastewater (omw) was tested on five perennial tree species. cupressus sempervirens and quercus ilex proved tolerant to six-month omw treatment followed by six-month water irrigation, whereas salix sp. and laurus nobilis and, later, pinus mugo suffered from phytotoxic effects. test plants were compared to controls after treatment and irrigation, by monitoring biochemical and microbiological variations in the rhizosphere soil. ...201021256739
flavonol-rich fractions of yaupon holly leaves (ilex vomitoria, aquifoliaceae) induce microrna-146a and have anti-inflammatory and chemopreventive effects in intestinal myofibroblast ccd-18co cells.polyphenolics extracted from yaupon holly (ilex vomitoria, aquifoliaceae) (yh) leaves were investigated in human colon cells for their chemopreventive and anti-inflammatory activities. an activity-guided fractionation allowed the selection of yh flavonol-rich fraction due to its preferential inhibition of ht-29 colon cancer viability over the normal ccd-18co colon cells. quercetin and kaempferol 3-rutinosides, main components identified in this fraction, protected ccd-18co cells against reactive ...201121262328
response of holm oak (quercus ilex subsp. ballota) and mastic shrub (pistacia lentiscus l.) seedlings to high concentrations of cd and tl in the rhizosphere.the impairment of root growth and photosynthetical functioning are the main impacts of trace elements on woody plant seedlings. in this work, we assessed the response of holm oak (quercusilex subsp. ballota) and mastic shrub (pistacia lentiscus) seedlings to high concentrations of cd and tl in the rhizosphere. these are non-essential trace elements, with a potential high mobility in the soil-plant system. seedlings of these species are frequently used in the afforestation of degraded soils in mi ...201121281955
technological characterization and stability of ilex paraguariensis st. hil. aquifoliaceae (maté) spray-dried powder.the present work was designed to produce an ilex paraguariensis spray-dried powder (sdp), in semi-industrial scale, in order to characterize its technological and chemical properties as well as to evaluate the thermal stability and photostability of the main polyphenol constituents. the yield of the spray-drying process was satisfactory (67%). the resulting sdp showed to be a material presenting spherical particles with a mean size of 19.6?µm, smooth surface, and good flow properties. the four p ...201121370969
the impact of packaging materials on the antioxidant phytochemical stability of aqueous infusions of green tea (camellia sinensis) and yaupon holly (ilex vomitoria) during cold storage.ready to drink (rtd) teas are a growing segment in the beverage category, brought about by improvements in the flavor of these products and healthy market trends driven by consumers. the presented results evaluated the antioxidant phytochemical stability of rtd teas from aqueous infusions of traditional green tea (camellia sinensis) and a botanical tea from yaupon holly (ilex vomitoria) as influenced by packaging materials during cold storage. two common packaging materials for rtd products are ...201121434687
two new 5,8-quinoflavans from the leaf of ilex centrochinensis.two new 5,8-quinoflavans were isolated from the leaf of ilex centrochinensis, and their structures were elucidated as (2r)-7,3',4'-trimethoxy-5,8-quinoflavan and (2s)-7-methoxy-4'-hydroxy-5,8-quinoflavan on the basis of spectroscopic methods, especially 1d and 2d nmr, cd, and mass spectral analyses. both of them exhibited weak cytotoxic activity against huh7 cell lines and no cytotoxic activity against caco-2 cell lines.201121462037
flavans from the leaf of ilex centrochinensis.phytochemical investigation of the leaf of ilex centrochinensis led to the isolation and characterization of four flavans, one flavanone (5), and one flavone (6), including a new compound whose structure was elucidated as (2s)-5,3',4'-trihydroxy-7-methoxyflavan (1) and a new natural product whose structure was elucidated as (2s)-5-hydroxy-7,3',4'-trimethoxyflavan (4) on the basis of spectroscopic methods especially 1d and 2d nmr, cd, and mass spectral analyses. compounds 1 and 4 exhibited weak c ...201121462042
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