Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| characterization of a new non-toxic two-chain ribosome-inactivating protein and a structurally-related lectin from rhizomes of dwarf elder (sambucus ebulus l.). | a new n-glycosidase ribosome-inactivating protein (rip) belonging to the novel family of the nontoxic type 2 rips from sambucaceae has been isolated from rhizomes of dwarf elder (sambucus ebulus l.) and named ebulin r. dwarf elder rhizomes also contain a novel monomeric n-ac-galactosamine-binding lectin that we named seaii. ebulin r and seaii have two isoforms each one, which were readily resolved by ion exchange. both isoforms of ebulin (ebulins r1 and r2) strongly inhibited protein synthesis i ... | 1997 | 9220142 |
| glycodelin from seminal plasma is a differentially glycosylated form of contraceptive glycodelin-a. | glycodelin-a is a human amniotic fluid-derived glycoprotein with contraceptive and immunosuppressive activities. an immunoreactive form of glycodelin was detected in seminal plasma over a decade ago, but definitive characterization of this glycoprotein was not pursued. we considered it unlikely that the seminal plasma of fertile men would contain an appreciable amount of contraceptive glycodelin-a. to address this issue we purified seminal plasma glycodelin (glycodelin-s) and performed comparati ... | 1996 | 9239694 |
| cbp70, a glycosylated nuclear lectin. | some years ago, a lectin designated cbp70 that recognized glucose (glc) but had a stronger affinity for n-acetylglucosamine (glcnac), was first isolated from hl60 cell nuclei. recently, a cytoplasmic form of this lectin was described, and one 82 kda nuclear ligand was characterized for the nuclear cbp70. in the present study, the use of pronase digestion and the trifluoromethanesulphonic acid (tfms) procedure strongly suggest that the nuclear and the cytoplasmic cbp70 have a same 23 kda polypept ... | 1997 | 9257193 |
| isolation and partial characterization of a novel and uncommon two-chain 64-kda ribosome-inactivating protein from the bark of elder (sambucus nigra l.). | a novel, strongly basic, two-chain ribosome-inactivating protein (rip) with an apparent mr of 64000 by sds-page and 63469 by mass spectrometry analysis, that we have named basic nigrin b, has been found in the bark of elder (sambucus nigra l.). the new protein does not agglutinate red blood cells, even at high concentrations and displays an unusually and extremely high activity towards animal ribosomes (ic50 of 18 pg/ml for translation by rabbit reticulocyte lysates). however, it is inactive aga ... | 1997 | 9287122 |
| inhibitory effects of turkish folk remedies on inflammatory cytokines: interleukin-1alpha, interleukin-1beta and tumor necrosis factor alpha. | in this study, in vitro inhibitory effects of 55 extracts or fractions obtained from 10 plant species on interleukin-1 (il-1alpha, il-1beta) and tumor necrosis factor (tnf-alpha) biosynthesis were studied. the following plant materials from turkish folk medicine for the treatment of various diseases which are thought to be inflammatory in nature e.g. rheumatism, fever, infections, edemas or related inflammatory diseases were selected as the subject of this study: cistus laurifolius leaves, clema ... | 1997 | 9324006 |
| increased alpha2,6 sialylation of n-glycans in a transgenic mouse model of hepatocellular carcinoma. | liver cancer is one of the most frequent and lethal malignancies worldwide. early detection is hampered by the absence of reliable markers. mice transgenic for the sv40 large t antigen under the control of a liver-specific promoter spontaneously develop well-differentiated hepatocellular carcinomas between 8 to 10 weeks of age. they are excellent models to investigate the alterations of protein expression in the early stages of tumor development and to follow these changes during tumor progressi ... | 1997 | 9331085 |
| interferon-gamma enhancement of e-selectin expression on endothelial cells is inhibited by monensin. | the expression of e-selectin reaches a maximum 4-6 h after stimulation of human umbilical vein endothelial cells (huvec) in vitro with tumour necrosis factor-alpha (tnf-alpha) and then declines to basal level within 24 h. if interferon-gamma (ifn-gamma) is added to the cell culture medium together with tnf-alpha the surface expression of e-selectin is augmented and prolonged in a synergistic way. the aim of the present study was to investigate if altered protein glycosylation could explain the i ... | 1997 | 9350283 |
| alterations in the o-linked glycosylation of iga1 in children with henoch-schönlein purpura. | to examine o-linked glycosylation of serum iga1 in children with acute henoch-schönlein purpura (hsp). | 1997 | 9375892 |
| identification of n-acetylneuraminic acid and its 9-o-acetylated derivative on the cell surface of cryptococcus neoformans: influence on fungal phagocytosis. | sialic acids from sialoglycoconjugates present at the cell surface of cryptococcus neoformans yeast forms were analyzed by high-performance thin-layer chromatography, binding of influenza a and c virus strains, enzymatic treatment, and flow cytofluorimetry with fluorescein isothiocyanate-labeled lectins. c. neoformans yeast forms grown in a chemically defined medium contain n-acetylneuraminic acid and its 9-o-acetylated derivative. a density of 3 x 10(6) residues of sialic acid per cell was foun ... | 1997 | 9393779 |
| inhibition of several strains of influenza virus in vitro and reduction of symptoms by an elderberry extract (sambucus nigra l.) during an outbreak of influenza b panama. | a standardized elderberry extract, sambucol (sam), reduced hemagglutination and inhibited replication of human influenza viruses type a/shangdong 9/93 (h3n2), a/beijing 32/92 (h3n2), a/texas 36/91 (h1n1), a/singapore 6/86 (h1n1), type b/panama 45/90, b/yamagata 16/88, b/ann arbor 1/86, and of animal strains from northern european swine and turkeys, a/sw/ger 2/81, a/tur/ger 3/91, and a/sw/ger 8533/91 in madin-darby canine kidney cells. a placebo-controlled, double blind study was carried out on a ... | 1995 | 9395631 |
| characterization of terminal neunacalpha2-3galbeta1-4glcnac sequence in lipooligosaccharides of neisseria meningitidis. | group b and c neisseria meningitidis are the major cause of meningococcal disease in the united states and in europe. n . meningitidis lipooligosaccharide (los), a major surface antigen, can be divided into 12 immunotypes of which l1 through l8 were found among group b and c organisms. groups b and c but not group a may sialylate their loss with n-acetylneuraminic acid (neunac) at the nonreducing end because they synthesize cmp-neunac. using sialic acid-galactose binding lectins as probes in an ... | 1998 | 9499383 |
| tyrosine phosphorylation following lectin mediated endothelial cell stimulation. | terminal alpha (1,3) galactosyl galactoside epitopes (alpha-gal) on membrane glycoproteins expressed by vascular endothelial cells represent the major xenoreactive antigens in pig to primate xenotransplantation. in other discordant xenotransplantation combinations, such as from guinea pig to rat, carbohydrate epitopes other than alpha-gal may be targeted by xenoreactive antibodies (xna). we have shown that agonist binding to alpha-gal epitopes induces proinflammatory activation of porcine aortic ... | 1998 | 9507735 |
| characterization of a spleen sulphotransferase responsible for the 6-o-sulphation of the galactose residue in sialyl-n-acetyl-lactosamine sequences. | an enzyme which catalyses the transfer of sulphate from 3'-phosphoadenosine 5'-phosphosulphate (paps) to c-6 of galactose in the neuacalpha2-3galbeta1-4glcnac (3'sln) sequence has been found in rat spleen microsomes and its specificity indicates that it is well suited to participate in the assembly of 3'-sialyl-6'-sulpho-lacnac [neuacalpha2-3gal(6-so4)beta1-4glcnac] and 3'-sialyl-6'-sulpho-lewisx [neuacalpha2-3gal(6-so4)beta1-4(fucalpha1-3)glcnac] saccharide groups which have been implicated as ... | 1998 | 9512489 |
| the generation and characterization of a rat neural cell line overexpressing the alpha2,6(n) sialyltransferase. | in order to examine the effects of altered protein sialylation on neural cell function, b104 rat neuroblastoma cells were stably transfected with the cdna coding for alpha2,6(n) sialyltransferase (st(6)n). lectin blot analysis of the clones demonstrated an increase in staining of the sambucus nigra lectin, which detects alpha2,6 linked sialic acid, in parallel with enzyme activity. there was a concomitant decrease in staining by the maackia amurensis lectin which labels alpha2,3-linked sialic ac ... | 1998 | 9557882 |
| histochemical analysis of sialic acids in the epididymis of the rat. | a variety of sialic acids contained in the rat epididymis were histochemically examined by means of lectin and pre-lectin methods by light microscopy. epididymides from adult male sprague-dawley rats were fixed in bouin's fluid and routinely embedded in paraffin wax. hydrated sections were subjected either to the lectin methods using biotinylated limax flavus, sambucus nigra, sambucus sieboldiana or maackia amurensis lectins or to the selective periodate oxidation-phenylhydrazine-thiocarbohydraz ... | 1998 | 9562390 |
| constitutive and inducible type 1 ribosome-inactivating proteins (rips) in elderberry (sambucus nigra l.). | two novel highly basic type 1 (single chain) ribosome-inactivating proteins (rips) with n-glycosidase activity have been found in elderberries (the fruits of sambucus nigra l.). mass spectrometry of these rips, which we named nigritins f1 and f2, gave mr values of 24095 and 23 565, respectively. both proteins strongly inhibited protein synthesis in rabbit reticulocyte lysates but were inactive against plant ribosomes. both nigritins have a similar topological activity on pbluescript sk+ dna as t ... | 1998 | 9645479 |
| does glycosylation of lysosomal proteins show age-related changes in rat liver? | we studied the pattern of lectins binding by liver lysosomal proteins from rats between 18 days of gestation and 72 weeks of age. an analysis of the carbohydrate structure was carried out after an electrophoresis and blotting, followed by a very sensitive detection system with highly specific digoxigenin-labelled lectins. the only age-related differences were observed in the reaction with sialic acid--(maa; macckia amurensis, sna; sambucus nigra) and fucose--(aaa; aleuria aurantia) specific lect ... | 1998 | 9663790 |
| mesenchymal changes associated with retinoic acid induced cleft palate in cd-1 mice. | retinoic acid (ra) is teratogenic in many species and is an effective inducer of cleft palate in mice. the pathogenesis of cleft formation varies with the timing of exposure. it has been demonstrated, before formation of the palatal shelves, that ra exposure results in insufficient mesenchymal tissue, and palatal shelves fail to make contact. however, at the palatal shelf outgrowth stage, ra exposure affects shelf elevation and growth in rats, and possibly medial edge epithelium (mee) differenti ... | 1998 | 9672841 |
| characterization of spacr, a sialoprotein associated with cones and rods present in the interphotoreceptor matrix of the human retina: immunological and lectin binding analysis. | rod and cone photoreceptors project from the outer retinal surface into a carbohydrate-rich interphotoreceptor matrix (ipm). unique ipm glycoconjugates are distributed around rods and cones. wheat germ agglutinin (wga) strongly decorates the rod matrix domains and weakly decorates the cone matrix domains. this study characterizes the major wga-binding glycoprotein in the human ipm, which we refer to as spacr (sialoprotein associated with cones and rods). spacr, which has a molecular weight of 14 ... | 1998 | 9719680 |
| role of sialic acid in the endocytosis of prosaposin by the nonciliated cells of the rat efferent ducts. | the present study examines the mechanism of endocytosis of testicular prosaposin by the nonciliated cells of the efferent ducts. testicular prosaposin is secreted by sertoli cells into the lumen of the seminiferous tubules as a 70 kda isomer where it binds to the tail of spermatozoa. in the efferent ducts, after dissociating from the plasma membrane of the spermatozoa, prosaposin is endocytosed by the nonciliated cells, presumably by receptor-mediated endocytosis. the initial step of receptor-me ... | 1998 | 9740323 |
| histochemical localization of glycoconjugates on microglial cells in alzheimer's disease brain samples by using abrus precatorius, maackia amurensis, momordica charantia, and sambucus nigra lectins. | four lectins (abrus precatorius (apa), maackia amurensis (maa), momordica charantia (mca) and sambucus nigra (sna)) have been used to identify glycohistochemically the microglial cells (mgc) activation in autoptic brain samples from alzheimer's disease (ad) subjects. three of these lectins (apa, maa and mca) have utilized as microglial cell markers for the first time. the identification of new markers for the study of mgc is very important to better understand the role of these type of cells in ... | 1998 | 9743580 |
| effects of pge2 and of different synthetic pge derivatives on the glycosylation of pig gastric mucins. | the glycosylation of pig gastric mucins, discharged in response to prostaglandin (pg) e2 and to three synthetic pge-derivatives (misoprostol, nocloprost, rioprostil) was compared. after a 20 h culture period in the absence or presence of 1 micromol/l of one of the pgs, mucins were isolated by gel chromatography and their glycosylation characterized by their linkage to a panel of lectins. for all tested pgs, a significantly increased lectin linkage to mucin glycoproteins of high molecular weight ... | 1998 | 9758207 |
| ribosome inactivating protein and lectin from bitter melon (momordica charantia) seeds: sequence comparison with related proteins. | one of the ribosome inactivating proteins (rips), beta-momorcharin, and a lectin were isolated from seeds of the bitter melon momordica charantia (family cucurbitaceae) in accordance with published procedures. both beta-momorcharin and m. charantia lectin were then subjected to amino acid sequencing. beta-momorcharin exhibited considerable homology in sequence to other cucurbitaceae rips, and also to the a chains of abrin and ricin which are type ii (double-chained) rips. the resemblance between ... | 1998 | 9875234 |
| localization and identification of galactose/n-acetylgalactosamine and sialic acid-containing proteins in chinese hamster metaphase chromosomes. | four lectins were used to recognize galactose/n-acetyl-galactosamine (gal/galnac) and sialic acid residues in proteins of chinese hamster metaphase chromosomes. in situ binding pattern of a fluorescein isothiocyanate-labelled (gal/galnac)-specific lectin sophora japonica agglutinin (sja) showed that chromosomal sja-binding proteins are primarily localized to the helically coiled substructure of chromatids. numerous sja-binding proteins were identified in western blots of chromosomal proteins, th ... | 1998 | 9878094 |
| analysis of 3- and 6-linked sialic acids in mixtures of gangliosides using blotting to polyvinylidene difluoride membranes, binding assays, and various mass spectrometry techniques with application to recognition by helicobacter pylori. | a convenient approach to analyze 3- and 6-linked sialic acids in mixtures of biologically active gangliosides was developed. the procedure was adapted to work on small amounts of material and included parallel tests, which allowed direct analysis of structure and activity. the initial step in the procedure was separation of a mixture of gangliosides by thin-layer chromatography (tlc) and blotting to a polyvinylidene difluoride membrane. the gangliosides were then analyzed (a) by direct desorptio ... | 1998 | 9882401 |
| differentiation elicits negative regulation of human beta-galactoside alpha2,6-sialyltransferase at the mrna level in the hl-60 cell line. | we studied the regulation of the beta-galactoside alpha2,6-sialyltransferase (hst6gal i) gene during hl-60 cell differentiation induced with dimethyl sulfoxide (dmso), all transretinoic acid (atra), and phorbol myristate acetate (pma). during hl-60 cell line differentiation, cell surface levels of alpha2,6-sialic acids expression decreased, as measured by flow cytometric analysis using sambucus nigra agglutinin (sna). activities of hst6gal i and levels of hst6gal i mrna dramatically decreased af ... | 1998 | 9883882 |
| mouse c127 cells transfected with fucosyltransferase fuc-tiii express masked lewisx but not lewisx antigen. | to study human alpha1,3/1,4fucosyltransferase (fuc-tiii) as an alpha1,3 fucosyltransferase, we constructed two cell clones, c127-ft and c127-t-ft, by transfecting cdna in parental (c127) or polyoma t antigen expressing (c127-t) mouse cells, respectively. both c127-ft and c127-t-ft clones express high levels of a fucosyltransferase activity kinetically similar to fuc-tiii and an rna that is amplified by a fuc-tiii-specific oligonucleotide primer pair after reverse transcription. clone c127-ft is ... | 1999 | 9884410 |
| s-fimbriae from escherichia coli bind to soluble glycoproteins from human milk. | escherichia coli (e. coli) strains, expressing s-fimbriae, belong to the most common gram-negative pathogens that cause sepsis and meningitis in neonates. the attachment of s-fimbriae to the cell surface is mediated by membrane glycoconjugates, which often carry n-acetylneuraminic acid. | 1999 | 10067725 |
| dietary lectins can induce in vitro release of il-4 and il-13 from human basophils. | dietary lectins, present in beans and other edible plant products, pose a potential threat to consumers due to their capacity to induce histamine release from basophils. in this study, we analyzed the capacity of 16 common, in particular dietary, lectins to induce human basophils to secrete il-4 and il-13, the key promoters of th2 responses and ige synthesis. several of the lectins, especially concanavalin a, lentil lectin, phytohemagglutinin, pisum sativum agglutinin and sambucus nigra agglutin ... | 1999 | 10092096 |
| cell-surface sialoglycoconjugate structures in wild-type and mutant crithidia fasciculata. | the cell-surface expression of sialoglycoconjugate structures in wild-type crithidia fasciculata and its tfr(r1) drug-resistant mutant was analyzed with the aid of an influenza c virus strain, lectin, enzymatic treatment, and flow cytofluorimetry analysis probed with fluorescein isothiocyanate-labeled (fitc) lectins. 9-o-acetyl-n-acetyl neuraminic acid (neu5,9ac2) structures mediate influenza c virus cell-binding. the saalpha2,3gal and saalpha2,6gal sequences are specifically recognized by maack ... | 1999 | 10099011 |
| differential expression of the hepatic transcript of beta-galactoside alpha2,6-sialyltransferase in human colon cancer cell lines. | the activity of beta-galactoside alpha2,6-sialyltransferase (st6gal.1), the enzyme responsible for the addition of sialic acid in alpha2,6-linkage to n-acetyllactosaminic (gal beta1,4glcnac) units of glycoconjugates, is increased in the vast majority of colon cancer specimens, and a positive correlation with an invasive phenotype has been suggested by several studies. in many tissues, st6gal.1 is regulated mainly at the transcriptional level through the use of different cell-specific promoters w ... | 1999 | 10188726 |
| effect of extracts of orthosiphon stamineus benth, hieracium pilosella l., sambucus nigra l. and arctostaphylos uva-ursi (l.) spreng. in rats. | aqueous extracts of sambucus nigra and arctostaphylos uva-ursi and hydroalcohol extracts of orthosiphon stamineus and hieracium pilosella were tested for their diuretic activities in rats; pharmacological evaluation revealed that they led to an increase in urine flow. urinary sodium excretion in rats was increased with o. stamineus and s. nigra. | 1999 | 10353162 |
| sialylation of e-cadherin does not change the spontaneous or et-18-ome-mediated aggregation of mcf-7 human breast cancer cells. | we have investigated the role of sialylation on cell-cell adhesion mediated by e-cadherin. two mcf-7 human breast cancer cell variants were studied: mcf-7/az cells showed a spontaneous cell-cell adhesion in the fast and slow aggregation assay. whereas the adhesion deficient mcf-7/6 cell variant failed to form larger aggregates, suggesting that e-cadherin was not functional under the conditions of both assays. we measured the sialyltransferase activities using galbeta1-3galnacalpha-o-benzyl and g ... | 1999 | 10432010 |
| composition of the sugar moiety of tamm-horsfall protein in patients with urinary diseases. | the sugar moiety of tamm-horsfall protein (thp) is altered by pathological conditions. the aim of this study was to investigate the composition of thp glycans in urinary diseases. thp was isolated from the urine of patients with urinary tract infection (group a), glomerulonephritis or interstitial nephritis (group b), and bartter's syndrome (bs) (group c). monosaccharides, n-glycan profile, thp reactivity with specific lectins and some other proteins were analyzed. thp of patients from groups a, ... | 1999 | 10436264 |
| grading dysplasia in colorectal adenomas by means of the quantitative binding pattern determination of arachis hypogaea, dolichos biflorus, amaranthus caudatus, maackia amurensis, and sambucus nigra agglutinins. | the current study deals with the setting up of a new tool that enables the benign versus the malignant nature of colorectal adenomas to be determined accurately. the 2 objectives are to determine (1) whether adenomas should, or should not, be included in a 2- or a 3-tier grading system, and (2) whether severe dysplasias and carcinomas in situ share common or different biological characteristics. the levels of expression of different types of glycoconjugates were characterized in a series of 166 ... | 1999 | 10534165 |
| attachment sites for particles in the urinary tract. | the adherence of crystals to the surface of renal tubule epithelial cells is one of the initial events in the development of nephrolithiasis. the accumulation of crystalline material in the kidney will sooner or later result in the formation of a stone. calcium crystals occasionally are present in the urine of even healthy individuals, and mechanisms responsible for the selective attachment of crystals to the tubular epithelium of stone-forming individuals must exist. although several types of c ... | 1999 | 10541278 |
| sialoforms of dipeptidylpeptidase iv from rat kidney and liver. | dipeptidylpeptidase iv (dpp iv, cd26), a serine-type exo- and endopeptidase found in the cell surface membrane of many tissues, was employed as a model membrane glycoprotein to study the expression of sialoforms on cell surface glycoproteins. native, enzymatically active dpp iv was purified from plasma membranes of kidney and liver by lectin affinity chromatography in conjunction with crown ether anion exchange chromatography. the enzyme was gradient-eluted in continuous fractions, all showing a ... | 1999 | 10561454 |
| neu5acalpha3gal is part of the helicobacter pylori binding epitope in polyglycosylceramides of human erythrocytes. | the sialic acid dependent binding by the human pathogen helicobacter pylori to polyglycosylceramides of human erythrocytes was investigated. polyglycosylceramides, complex glycosphingolipids with a branched n-acetyllactosamine core, were isolated from human erythrocytes, blood group o, and subfractionated after peracetylation by anion-exchange chromatography. three subfractions were deacetylated, analysed by matrix-assisted laser desorption ionization-time of flight ms and 2d 1h nmr spectroscopy ... | 1999 | 10561598 |
| rat gastric mucous gel layer contains sialomucin not produced by the stomach. | the sialylated mucus components of the normal gastric mucosa and mucous gel layer of rats were studied by using various histochemical staining methods including maackia amurensis ii (mal-ii) and sambucus nigra (sna) lectins, alcian blue (ab) ph 2.5 -- periodic acid schiff (pas) and high iron diamine (hid) -- ab ph 2.5. the acidic and neutral mucins characterized by the ab-pas staining were abundantly present in the mucous gel layer as well as in the gastric mucosa. the sialomucin characterized b ... | 1999 | 10580375 |
| sialic acid residues in the labial salivary glands from sjögren's syndrome patients. | to investigate the composition and expression of sialic acid in the labial salivary glands (lsg) in sjögren's syndrome (ss). | 2017 | 10609070 |
| the traditional plant treatment, sambucus nigra (elder), exhibits insulin-like and insulin-releasing actions in vitro. | sambucus nigra (elder) has been documented as a traditional treatment of diabetes. in the present study, an aqueous extract of elder (aee, 1 g/l) significantly increased 2-deoxy-glucose transport, glucose oxidation and glycogenesis of mouse abdominal muscle in the absence of added insulin (2 x 2 factorial design). in acute 20-min tests, 0.25-1 g/l aee evoked a stepwise stimulation of insulin secretion from clonal pancreatic beta-cells. the insulin releasing effect of aee (0.5 g/l) was significan ... | 2000 | 10613759 |
| endothelial glycoconjugates: a comparative lectin study of the brain, retina and myocardium. | there is evidence that the endothelial cell (ec) glycocalyx is a significant determinant of vascular permeability, acting as a charge-size filter to permeant molecules. we have therefore examined its oligosaccharide composition in 3 classes of microvessel with differing permeabilities. ec in rat brain, retina and myocardium were labelled with a panel of lectins and subjected to a semiquantitative analysis. surprisingly, no substantial differences were evident for any lectin labelling between the ... | 2000 | 10697288 |
| lectin histochemical examination of rabbit bladder glycoproteins and characterization of a mucin isolated from the bladder mucosa. | the glycocalyx of the mucosal surface of urinary bladder acts as an effective barrier against invasion by pathogenic microorganisms and injury from toxic substances in the urine. defects in these bladder mucosal components could thus be important factors in the development of diseases such as interstitial cystitis and lower urinary tract infections. however, information on the nature of glycoconjugates of mammalian bladder mucosa is very limited. in this study, the glycoconjugates of rabbit blad ... | 2000 | 10700383 |
| sialic acid and crystal binding. | we studied the role of cell surface sialic acid in the adherence of calcium oxalate monohydrate (com) crystals to madin-darby canine kidney (mdck) cells. | 2000 | 10720959 |
| identification of sialic acids on the cell surface of candida albicans. | the cell-surface expression of sialic acids in two isolates of candida albicans was analyzed by thin-layer and gas chromatography, binding of lectins, colorimetry, sialidase treatment and flow cytofluorimetry with fluorescein-labeled lectins. n-acetylneuraminic acid (nana) was the only derivative found in both strains of c. albicans grown in a chemically defined medium. its identification was confirmed by mass spectrometry in comparison with an authentic standard. the density of sialic acid resi ... | 2000 | 10742607 |
| immunohistochemical analysis of sialic acid and fucose composition in human colorectal adenocarcinoma. | the expression of different sialoglycoconjugates and fucoglycoconjugates in normal mucosa and adenocarcinoma samples from 43 colorectal cancer patients was investigated by using specific lectins and applying a semiquantitative analysis. a pronounced decrease in the intracellular binding of the maackia amurensis lectin, which recognizes alpha(2,3)-linked sialic acid residues, was found in the tumoral tissue. in contrast, a significant increase in the staining with the sambucus nigra lectin (sna i ... | 2000 | 10754466 |
| terminal glycosylation of bovine uroplakin iii, one of the major integral-membrane glycoproteins of mammalian bladder. | uroplakin iii (upiii) is one of the major transmembrane glycoproteins exposed at the luminal face of mammalian bladder. we investigated the terminal glycosylation of bovine upiii in order to ascertain whether it contains the alpha 2,3-sialylated sequence thus potentially serving as a receptor for uropathogenic escherichia coli expressing type s adhesins. we report the occurrence of sialic acid in alpha 2,3- and alpha 2,6-linkage to galactose in bovine upiii glycans as evidenced by the sensitivit ... | 2000 | 10913821 |
| beta-galactoside alpha2,6 sialyltransferase in human colon cancer: contribution of multiple transcripts to regulation of enzyme activity and reactivity with sambucus nigra agglutinin. | colon cancer tissues display an increased activity of beta-galactoside alpha2,6 sialyltransferase (st6gal.i) and an increased reactivity with the lectin from sambucus nigra (sna), specific for alpha2,6-sialyl-linkages. experimental and clinical studies indicate a contribution of these alterations to tumor progression, but their molecular bases are largely unknown. in many tissues, st6gal.i is transcriptionally regulated through the usage of different promoters that originate mrnas diverging in t ... | 2000 | 10962440 |
| effect of signaling inhibitors on the release of lysozyme from human neutrophils activated by sambucus nigra agglutinin. | the effect of alpha-neuac(2-->6)gal/galnac-specific lectin from sambucus nigra (sna) on the release of lysozyme from human neutrophils was studied in vitro. interaction of cells with the lectin was accompanied by dose-dependent release of lysozyme, which was increased in the presence of cytochalasin b. the involvement of intracellular signaling pathways in the lectin-induced degranulation of neutrophils was determined using a panel of specific inhibitors tested at concentrations in the range of ... | 2000 | 11002187 |
| the presence of n-acetylneuraminic acid in malpighian tubules of larvae of the cicada philaenus spumarius. | sialic acid-containing glycoconjugates are generally considered to be unique to the deuterostomes, a lineage of the animal kingdom which includes animals from the echinoderms up to the vertebrates. there are, however, two isolated reports of sialic acid occurring in the insect species drosophila melanogaster and galleria mellonella. since insects are classified as protostomes, these findings call previous assumption on the phylogenetic distribution and thus on the evolution of sialic acids into ... | 1999 | 11003558 |
| reaction of various lectins to mucin derived from the different layers of rat gastric mucosa: comparison of enzyme-linked lectin binding assay with lectin histochemistry. | the purpose of this study was to demonstrate that the histochemical staining reactivities of lectins in rat stomach actually represent the gastric mucins, and to estimate the utility of the lectins for mucin histochemistry. in this paper, the lectin histochemistry was compared with an enzyme-linked lectin binding assay (ella) of the mucins derived from distinct regions and layers of the sprague-dawley rat stomach and it was examined to determine the definite binding and problematic binding of th ... | 2000 | 11041246 |
| lectin analysis of human immunoglobulin g n-glycan sialylation. | the lectins sambucus nigra agglutinin (sna) and ricinus communis agglutinin (rca), specific for alpha2,6 linked sialylation, and terminal galactose respectively were used to study the occurrence, linkage and distribution of human immunoglobulin g (igg) sialylation. sna was shown to bind n-glycan alpha2,6-linked sialic acid only. sialidase analysis confirmed that this is the dominant, if not exclusive linkage. total igg sialylation was estimated at 1.0 microg sa/mg igg (or about 0.5 mole per mole ... | 1999 | 11133020 |
| analysis of glycoconjugate patterns of normal and hormone-induced dysplastic noble rat prostates, and an androgen-independent noble rat prostate tumor, by lectin histochemistry and protein blotting. | alteration of the expression of glycoconjugates is frequently observed in tumors. however, studies on the changes of cellular glycosylation in the early premalignant stage of prostate carcinogenesis are scarce. | 2001 | 11170128 |
| analysis of anthocyane glycosides in human serum. | anthocyanins, natural food antioxidants, can be identified in human blood plasma using a restricted access phase for removal of the proteins and enrichment of the anthocyanins. in preliminary studies the spray dried elderberry juice was shown to have antioxidant activities in vitro. from the four known anthocyanins present in elderberry (sambucus nigra) the two main components could be analysed quantitatively enabling their analysis in blood. using a restricted access phase and a column switchin ... | 2000 | 11220323 |
| hypersialylated macromolecular serum immunoglobulin a1 in type 2 diabetes mellitus. | the origin of the elevation of serum immunoglobulin a1 (iga1) in type 2 diabetes mellitus (dm) is unsettled. the aim of this study was to address the carbohydrate changes of serum iga1 from patients with type 2 diabetes mellitus, as a possible cause of the elevation. | 2001 | 11239513 |
| n-acetylgalactosamine, n-acetylglucosamine and sialic acid expression in primary breast cancers. | binding of the lectin from helix pomatia (hpa), which recognises n-acetylgalactosamine and n-acetylglucosamine glycans, is a predictor of metastasis and poor prognosis in a number of human adenocarcinomas, including breast cancer. the glycoproteins to which it binds in these tumours have been only partially characterised, and the mechanisms underlying their biosynthesis remain unknown. in this study, 111 primary breast cancers were assessed for binding of hpa and labelling characteristics were c ... | 2001 | 11252626 |
| differences in sialic acid density in pathogenic and non-pathogenic aspergillus species. | aspergillus: fumigatus is a ubiquitous soil fungus that causes invasive lung disease in the immunocompromised host. the structure of the conidial wall has not been well characterized although it is thought that adhesins present on the surface are involved in attachment of the conidia to host lung cells and proteins, which is a prerequisite for the establishment of infection. negatively charged carbohydrates on the conidial surface have been previously identified as the molecules responsible for ... | 2001 | 11283283 |
| the effect of sambucol, a black elderberry-based, natural product, on the production of human cytokines: i. inflammatory cytokines. | sambucus nigra l. products - sambucol - are based on a standardized black elderberry extract. they are natural remedies with antiviral properties, especially against different strains of influenza virus. sambucol was shown to be effective in vitro against 10 strains of influenza virus. in a double-blind, placebo-controlled, randomized study, sambucol reduced the duration of flu symptoms to 3-4 days. convalescent phase serum showed a higher antibody level to influenza virus in the sambucol group, ... | 2001 | 11399518 |
| suppression of a sialyltransferase by antisense dna reduces invasiveness of human colon cancer cells in vitro. | transfer of terminal alpha 2,6-linked sialic acids to n-glycans is catalyzed by beta-galactoside alpha 2,6-sialyltransferase (st6gal i). expression of st6gal i and its products is reportedly increased in colon cancers. to investigate directly the functional effects of st6gal i expression, human colon cancer (ht29) cells were transfected with specific antisense dna. st6gal i mrna and protein were virtually undetectable in six strains of transfected ht29 cells. st6gal activity was reduced to 14% o ... | 2001 | 11406350 |
| the interaction of selective plant lectins with neuraminidase-treated and untreated iga1 from the sera of iga nephropathy patients. | a study on the binding interaction of lectins from artocarpus heterophyllus (jacalin), glycine max and sambucus nigra with standardised quantity of iga from the iga nephropathy patients and normal controls was performed. the glycine max lectin demonstrated higher affinity towards the serum iga of igan patients as compared to normal controls. however, the affinity binding was lower in cases ofjacalin and the sambucus nigra lectin. when serum samples were treated with neuraminidase, the differenti ... | 2001 | 11419909 |
| comparison of the lectin-binding pattern in different human melanoma cell lines. | glycosylation is generally altered in tumour cells in comparison with their normal counterparts. these alterations are thought to be important because they contribute to the abnormal behaviour of cancer cells. therefore, we have comparatively analysed the glycoproteins in cell extracts from human melanoma (primary site--wm35; metastatic sites-- wm239, wm9 and a375) cell lines using sodium dodecyl sulphate-polyacrylamide gel electrophoresis and lectin staining. the glycoprotein pattern of the wm3 ... | 2001 | 11468508 |
| preparation of monospecific polyclonal antibodies against sambucus nigra lectin related protein, a glycosylated plant protein. | a simple, but highly efficient, method was developed for the purification of monospecific antibodies against the plant glycoprotein sambucus nigra lectin related protein. in a first step, the antiserum is purified by affinity chromatography on a column with the immobilized antigen. to deplete the affinity-purified antiserum from aspecific cross-reacting antibodies directed against the glycan part of the glycoprotein, a second affinity chromatography on an unrelated plant glycoprotein, in casu th ... | 2001 | 11513087 |
| a novel tumor-associated mucin of gastrointestinal carcinoma. | to identify a new tumor-associated antigen, a monoclonal antibody, sc142, was produced by immunizing mice with a stomach cancer cell line. the tumor specificity of mab sc142 was studied by immunohistochemical staining, and the biochemical characteristics of this new gastrointestinal tumor-associated antigen were also studied. | 2001 | 11570576 |
| application of a lectin from the mushroom polysporus squamosus for the histochemical detection of the neuacalpha2,6galbeta1,4glc/glcnac sequence of n-linked oligosaccharides: a comparison with the sambucus nigra lectin. | the lectin from the mushroom polysporus squamosus (psl) has an extended carbohydrate combining site, which exhibits a high specificity and affinity toward the neuac5alpha2,6galbeta1,4glc/glcnac trisaccharide sequence of asparagine-linked oligosaccharides. therefore, psl should be a superior reagent to the lectin from sambucus nigra (sna), which does not discriminate between alpha2,6-linked neuac5 present either in asparagine- or serine/threonine-linked oligosaccharides. we have prepared a digoxi ... | 2001 | 11685546 |
| potential allelochemicals from sambucus nigra. | twenty-four aromatic metabolites belonging to cyanogenins, lignans, flavonoids, and phenolic glycosides were obtained from sambucus nigra. structures were determined on the basis of their spectroscopic features. two compounds have been isolated and identified as (2s)-2-o-beta-d-glucopyranosyl-2-hydroxyphenylacetic acid and benzyl 2-o-beta-d-glucopyranosyl-2,6-dihydroxybenzoate. all the compounds have been assayed on dicotyledons lactuca sativa (lettuce) and raphanus sativus (radish) and monocoty ... | 2001 | 11730871 |
| comparison of structural changes of cell surface carbohydrates in the eustachian tube epithelium of chinchillas infected with a streptococcus pneumoniae neuraminidase-deficient mutant or its isogenic parent strain. | six different lectin probes were used to examine alterations of the cell surface carbohydrates in the chinchilla eustachian tube (et) lumen subsequent to the intranasal (i.n.) challenge with the streptococcus pneumoniae parent strain, d39, or its isogenic derivative, deltana1, which is deficient in neuraminidase nana. the labelling pattern revealed that the binding of wheat germ agglutinin (wga), erythrina cristagalli lectin (ecl), peanut agglutinin (pna), bandeiraea simplicifolia lectin ii (bsl ... | 2001 | 11747378 |
| region-specific ontogeny of alpha-2,6-sialyltransferase during normal and cortisone-induced maturation in mouse intestine. | regional differences in the ontogeny of mouse intestinal alpha-2,6-sialyltransferase activities (alpha-2,6-st) and the influence of cortisone acetate (ca) on this expression were determined. high st activity and alpha-2,6-st mrna levels were detected in immature small and large intestine, with activity increasing distally from the duodenum. as the mice matured, st activity (predominantly alpha-2,6-st) in the small intestine decreased rapidly to adult levels by the fourth postnatal week. ca preco ... | 2002 | 11841998 |
| glycoproteins from the cuticle of the atlantic shore crab carcinus maenas: i. electrophoresis and western-blot analysis by use of lectins. | the protein and glycoprotein content of four different neutral or acidic solvent extracts (0.5 m kcl, 10% edta, 0.1 n hcl, or 2% acetic acid) from the mineralized exoskeleton of a decapod crustacean, the atlantic shore crab carcinus maenas, were characterized by quantitative analysis of proteins, sds-page analysis, and probing with lectins on blots. the lectins used were conconavalin a, jacalin, soybean agglutinin, maackia amurensis agglutinin ii, and sambucus nigra agglutinin. the results show ... | 2002 | 11842016 |
| altered sialyl- and fucosyl-linkage on mucins in cystic fibrosis patients promotes formation of the sialyl-lewis x determinant on salivary muc-5b and muc-7. | destruction of the lungs as a consequence of recurrent infections with microorganisms such as pseudomonas aeruginosa remains the underlying cause of most morbidity and mortality in cystic fibrosis (cf). we have hypothesized that changes in the glycosylation of key tracheal mucins such as muc5b and muc7 might increase the risk of pulmonary disease in cf patients. however, in preference to sputum we have examined the sugar-chains on these mucins in saliva because in the latter not only can the gly ... | 2001 | 11845304 |
| anti-hiv activity of a glycoprotein from first trimester placental tissue. | human immunodeficiency virus-1 (hiv-1) transmission from a mother to the offspring during pregnancy is highly complex and a variable process in part due to the presence of several anti-hiv factors. in this study, we report an early pregnancy-associated protein (early pregnancy associated protein-1, epap-1) with an apparent molecular weight of 90 kda in placental tissue of pregnant women during the first trimester. epap-1 was isolated and purified using sambucus nigra agarose lectin affinity chro ... | 2002 | 11888657 |
| specific lectin binding to beta1 integrin and fibronectin on the apical membrane of madin-darby canine kidney cells. | although lectins have previously been used to identify specific cell types in the kidney and various other tissues, the proteins labeled were not identified. we hypothesized that fluorescently labeled lectins could provide a useful tool for direct labeling of membrane-associated glycoproteins. protein fractions from madin-darby canine kidney (mdck) cells were exposed to a panel of 16 fluorescently labeled lectins to identify suitable lectin-protein pairs. peanut agglutinin (pna) selectively boun ... | 2002 | 11891552 |
| biochemical, molecular and structural analysis of multiple thaumatin-like proteins from the elderberry tree (sambucus nigra l.). | thaumatin-like proteins (tlps) were isolated and characterized from fruits and leaves of elderberry (sambucus nigra) and their corresponding genes cloned. in addition, the developmental regulation and induction of the different tlps was followed in some detail. ripening berries accumulated a fruit-specific tlp during the final stages of maturation. this fruit-specific tlp had no antifungal activity and was devoid of beta-glucanase activity. leaves constitutively expressed a tlp that closely rese ... | 2002 | 11941461 |
| the sambucus nigra type-2 ribosome-inactivating protein sna-i' exhibits in planta antiviral activity in transgenic tobacco. | transgenic tobacco (samsun nn) plants transformed with a cdna clone encoding sna-i' from sambucus nigra synthesize, and correctly process and assemble, a fully active type-2 ribosome-inactivating protein. expression of sna-i' under the control of the 35s cauliflower mosaic virus promoter enhances the plant's resistance against infection with tobacco mosaic virus. in contrast to type-1 ribosome-inactivating proteins, the expression of sna-i' does not affect the growth and fertility of the transge ... | 2002 | 11959096 |
| specific lectins map the distribution of fibronectin and beta 1-integrin on living mdck cells. | the expression and dynamics of bound fibronectin and the sialylated integral membrane protein, beta 1-integrin, were analyzed on the apical membrane of living mdck cells. fibronectin was identified by its specific binding of fluorescent peanut agglutinin and sialylated beta 1-integrin by its binding of sambucus nigra agglutinin. confocal epifluorescence microscopy and laser scanning cytometry determined the distribution and abundance of binding sites of the two fluorescently labeled lectins. bot ... | 2002 | 11978008 |
| mutational analysis of the carbohydrate-binding activity of the neuac(alpha-2,6)gal/galnac-specific type 2 ribosome-inactivating protein from elderberry (sambucus nigra) fruits. | sambucus nigra agglutinin i (sna-i) is a type 2 ribosome-inactivating protein. site-directed mutagenesis was used to mimic the conversion of the highly active b-chain of fruit-specific sna (sna-if) into the completely inactive b-chain of the closely related and naturally occurring loss-of-activity mutant called s. nigra agglutinin lectin-related protein. in the first mutant sna-if-m1 the high-affinity site 2 of sna-if was disrupted by replacing the presumed critical residue asp231 with glu231. i ... | 2002 | 12023903 |
| accumulation of plant small heat-stress proteins in storage organs. | plant small heat-stress proteins (shsps) have been shown to be expressed not only after exposure to elevated temperatures, but also at particular developmental stages such as embryogenesis, microsporogenesis, and fruit maturation. this paper presents new data on the occurrence of shsps in vegetative tissues, their tissue-specific distribution, and cellular localization. we have found shsps in 1-year-old twigs of acer platanoides l. and sambucus nigra l. and in the liana aristolochia macrophylla ... | 2002 | 12029471 |
| a complex fruit-specific type-2 ribosome-inactivating protein from elderberry (sambucus nigra) is correctly processed and assembled in transgenic tobacco plants. | fruits of elderberry (sambucus nigra) express small quantities of a type-2 ribosome-inactivating protein with an exclusive specificity towards the neuac(alpha2,6)gal/galnac disaccharide and a unique molecular structure typified by the occurrence of a disulfide bridge between the b-chains of two adjacent protomers. a cdna clone encoding this so-called sambucus nigra fruit specific agglutinin i (sna-if) was isolated and expressed in tobacco (samsun nn) under the control of the 35s cauliflower mosa ... | 2002 | 12071953 |
| different enzyme activities of sialyltransferases in gynecological cancer cell lines. | due to rarity of a relationship between sialyltransferase enzyme activities and gynecological cancers, we arranged the study to evaluate sialyltransferase enzyme activity in the various kinds of gynecological cancer cell lines. | 2002 | 12094959 |
| comparison of alteration of cell surface carbohydrates of the chinchilla tubotympanum and colonial opacity phenotype of streptococcus pneumoniae during experimental pneumococcal otitis media with or without an antecedent influenza a virus infection. | experimental and clinical studies suggest that influenza a virus promotes streptococcus pneumoniae-induced otitis media; however, the mechanism underlying this synergistic interaction has not been completely defined. in this study, glycoconjugate expression patterns were evaluated on the cell surface in the chinchilla eustachian tube (et) lumen of a cohort challenged intranasally (i.n.) with s. pneumoniae type 6a, which is predominantly transparent and a cohort with an antecedent influenza a vir ... | 2002 | 12117938 |
| effect of neuraminidase on receptor-mediated adherence of streptococcus pneumoniae to chinchilla tracheal epithelium. | the trachea whole organ perfusion technique was used to study the effect of the disruption of the streptococcus pneumoniae neuraminidase nana gene on bacterial adherence and alteration of the carbohydrate surface structures of respiratory epithelial cells. six different lectin probes were used to examine alterations of the cell surface carbohydrates in chinchilla tracheal epithelium incubated in vitro with s. pneumoniae deltana1, a neuraminidase-deficient mutant, or its d39 parent strain. the la ... | 2002 | 12125999 |
| ruptured achilles tendons show increased lectin stainability. | to ascertain whether lectins could be a useful tool for investigation of the extracellular matrix of degenerated and normal tendons. | 2002 | 12131241 |
| quantification of the bioadhesive properties of protein-coated pvm/ma nanoparticles. | this work describes the bioadhesive properties of poly(methyl vinyl ether-co-maleic anhydride) (pvm/ma) nanoparticles fluorescently-labelled with rhodamine b isothiocyanate, and coated with either sambucus nigra lectin (sna-np) or bovine serum albumin (bsa-np). the different formulations (10 mg) were administered to animals by the oral route and the fraction of adhered particles to the mucosa was estimated by measuring the fluorescent marker after the digestion of the tissue. plotting the amount ... | 2002 | 12176235 |
| different glycosylation of cadherins from human bladder non-malignant and cancer cell lines. | the aim of the present study was to determine whether stage of invasiveness of bladder cancer cell lines contributes to alterations in glycan pattern of their cadherins. | 2002 | 12234377 |
| analysis of glycoproteins in cell culture supernatants using a lectin immunosensor technique. | a method based on a surface plasmon resonance technique for detection of changes in concentration and glycosylation of proteins in cell culture supernatant is described. the method was used to analyze alpha(1)-acid glycoprotein (agp) produced by a human hepatoma cell line (hepg2). cell culture supernatant was injected to a biacore 2000 instrument and agp was captured on the sensor chip by immobilized antibodies. the captured glycoprotein was then analyzed for content of carbohydrate epitopes usi ... | 2002 | 12243907 |
| water and oxygen permeance of phellems isolated from trees: the role of waxes and lenticels. | the outermost phellems of abies alba mill., acer pseudoplatanus l., aesculus hippocastanum l., betula potaninii l.c. hue and sambucus nigra l. have been isolated enzymatically, resulting in membranes with five to seven heavily suberized cork cell layers. water and oxygen permeances were determined for the phellem areas without lenticels. a special diaphragm made it possible to quantify permeances of single lenticels for the first time. the water permeance of phellems was in the range of 3x10(-5) ... | 2002 | 12244445 |
| interaction of glycophorin a with lectins as measured by surface plasmon resonance (spr). | glycophorin a (gpa), the major sialoglycoprotein of the human erythrocyte membrane, was isolated from erythrocytes of healthy individuals of blood groups a, b and o using phenol-water extraction of erythrocyte membranes. interaction of individual gpa samples with three lectins (psathyrella velutina lectin, pvl; triticum vulgaris lectin, wga and sambucus nigra i agglutinin sna-i) was analyzed using a biacore biosensor equipped with a surface plasmon resonance (spr) detector. the experiments showe ... | 2002 | 12362990 |
| gantrez an as a new polymer for the preparation of ligand-nanoparticle conjugates. | the aim of this study was to evaluate the feasibility and in vitro activity of ligand-conjugates based on the use of poly(methyl vinyl ether-co-maleic anhydride) (pvm/ma or gantrez an). fluorescently labelled pvm/ma nanoparticles were prepared by desolvation and cross-linkage with 1,3-diaminopropane (dp). conjugates were obtained by incubation between the carriers and sambucus nigra agglutinin (sna) for 1 h in an aqueous medium. the lectin binding to the surface of nanoparticles was increased by ... | 2002 | 12387941 |
| effects of dietary anthocyanins on tocopherols and lipids in rats. | the effects of dietary cyanidin-3-o-glucoside (c3g) and concentrates from blackcurrant [ribes nigrum] (bc) and elderberry [sambucus nigra] (ec) on plasma and tissue concentrations of alpha- (alpha-t) and gamma-tocopherol (gamma-t) and cholesterol, as well as the fatty acid composition of the liver lipids were investigated in growing, male rats of the sprague-dawley strain. animals were fed semisynthetic diets supplemented with 2 g/kg c3g, bc, or ec for 4 weeks. dietary anthocyanins did not affec ... | 2002 | 12452636 |
| carbohydrate moieties of n-cadherin from human melanoma cell lines. | expression of n-cadherin an adhesion molecule of the cadherin family, in tumor cells is associated with their increased invasive potential. many studies suggested the role of n-linked oligosaccharides as important factors that contribute to metastasis by influencing tumor cell invasion and adhesion. n-cadherin is a heavily glycosylated protein. we have analysed the carbohydrate profile of this protein synthesized in human melanoma cell lines: wm35 from the primary tumor site and wm239, wm9, and ... | 2002 | 12545205 |
| changes in sialic acid expression in the lung during intrauterine development of the human fetus. | sialic acid is a component of glycoproteins that influences enzymatic and receptor functions of cells. during proliferation and differentiation of tissues, sialic acid can serve as a recognition determinant in intercellular communication and interactions of cells with the extracellular matrix. in the present study, sialic acid expression in relation to developmental maturity of the lung has been studied. we analyzed 12 necroptic lung specimens from foetuses of different gestational ages from the ... | 2002 | 12553698 |
| sialic acid expression in autoimmune thyroiditis. | autoimmune diseases of the thyroid gland are among the most frequent endocrine disorders. the present study analyzes expression patterns of sialic acids in these diseases. three lectins specific for sialic acids were used for the histochemical analysis of surgical specimens of the thyroid gland: tritrichomonas mobilensis lectin that stains all types of sialic acids, maackia amurensis leukoagglutinin that stains sialic acids with alpha2,3 linkage and sambucus nigra agglutinin that stains sialic a ... | 2002 | 12553699 |
| identification and functional expression of a second human beta-galactoside alpha2,6-sialyltransferase, st6gal ii. | blast analysis of the human and mouse genome sequence databases using the sequence of the human cmp-sialic acid:beta-galactoside alpha-2,6-sialyltransferase cdna (hst6gal i, ec2.4.99.1) as a probe allowed us to identify a putative sialyltransferase gene on chromosome 2. the sequence of the corresponding cdna was also found as an expressed sequence tag of human brain. this gene contained a 1590 bp open reading frame divided in five exons and the deduced amino-acid sequence didn't correspond to an ... | 2003 | 12603328 |
| galt deficiency causes udp-hexose deficit in human galactosemic cells. | previously we reported that stable transfection of human udp-glucose pyrophosphorylase (hugp2) rescued galactose-1-phosphate uridyltransferase (galt)-deficient yeast from "galactose toxicity." here we test in human cell lines the hypothesis that galactose toxicity was caused by excess accumulation of galactose-1-phosphate (gal-1-p), inhibition of hugp2, and udp-hexose deficiency. we found that sv40-transformed fibroblasts derived from a galactosemic patient accumulated gal-1-p from 1.2+/-0.4 to ... | 2003 | 12626383 |
| identification and characterization of adsorbed serum sialoglycans on leishmania donovani promastigotes. | sialic acids as terminal residues of oligosaccharide chains play a crucial role in several cellular recognition events. the presence of sialic acid on promastigotes of leishmania donovani, the causative organism of indian visceral leishmaniasis, was demonstrated by fluorimetric high-performance liquid chromatography showing neu5ac and, to a minor extent, neu5,9ac2. the presence of neu5ac was confirmed by gc/ms analysis. furthermore, binding with sialic acid-binding lectins sambucus nigra aggluti ... | 2003 | 12626423 |
| cell-surface matrix proteins and sialic acids in cell-crystal adhesion; the effect of crystal binding on the viability of human caki-1 renal epithelial cells. | to investigate the role of sialic acids and cellular matrix proteins as crystal-binding molecules in human calcium-oxalate nephrolithiasis. | 2003 | 12656914 |
| alterations of branching and differential expression of sialic acid on alpha-1-acid glycoprotein in human seminal plasma. | the degree of branching and types of fucosylation of glycans on alpha(1)-acid glycoprotein (agp) have been found to be associated with alpha(1)-acid glycoprotein concentrations in human seminal plasma. the glycosylation pattern of alpha(1)-acid glycoprotein in seminal plasma obtained from men living in infertile couples can undergo alterations in relation to sperm analysis and/or alpha(1)-acid glycoprotein concentrations. | 2003 | 12691868 |
| sialylglycoconjugates and sialyltransferase activity in the fungus cryptococcus neoformans. | cryptococcus neoformans is a fungal pathogen associated with systemic mycoses in up to 10% of aids patients. c. neoformans yeasts express sialic acids on the cell wall, where they play an anti-phagocytic role, and may represent a virulence factor at the initial phase of infection. since the nature of the sialic acid-carrying components is undefined in c. neoformans, our aim in the present work was to identify sialylated molecules in this fungus and study the sialylation process. c. neoformans ye ... | 2002 | 12815227 |
| correlation between the sialylation of cell surface thomsen-friedenreich antigen and the metastatic potential of colon carcinoma cells in a mouse model. | the cell surface glycosylation profiles of a liver metastatic colon carcinoma variant cell line, sl4 cells previously selected from colon 38 cells in vivo for liver colonization were investigated. flowcytometric analysis was performed with 7 plant lectins and 10 carbohydrate specific monoclonal antibodies. the results showed that peanut agglutinin (pna), sambucus nigra agglutinin, ulex europeus agglutinin-i, anti-lex, anti-ley, and anti-le(b) antibodies bound to the parental colon 38 cells but n ... | 2001 | 12820723 |
| differences in glycosylation and sperm-egg binding inhibition of pregnancy-related glycodelin. | glycodelin is a glycoprotein produced in many glands, particularly those of reproductive tissues. it appears as different glycoforms in amniotic fluid (glycodelin-a) and seminal plasma (glycodelin-s), but only glycodelin-a inhibits gamete adhesion. in the present study, glycodelin from secretory-phase endometrium, first-trimester pregnancy decidua, and midtrimester amniotic fluid was studied with respect to physicochemical properties, including glycosylation patterns and inhibitory activity of s ... | 2003 | 12826581 |
| overexpression of the alpha-2,6-sialyltransferase in mdck cells increases influenza virus sensitivity to neuraminidase inhibitors. | no reliable cell culture assay is currently available for monitoring human influenza virus sensitivity to neuraminidase inhibitors (nai). this can be explained by the observation that because of a low concentration of sialyl-alpha2,6-galactose (sia[alpha2,6]gal)-containing virus receptors in conventional cell lines, replication of human virus isolates shows little dependency on viral neuraminidase. to test whether overexpression of sia(alpha2,6)gal moieties in cultured cells could make them suit ... | 2003 | 12857911 |