Publications

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persistence, metabolism, and movement of ethylenethiourea in eggplant (solanum melongena l.) under subtropical conditions. 19938318773
the in vitro synthesis of diosgenin mono- and diglucoside by enzyme preparations from solanum melongena leaves. 19938372541
tropical rotation crops influence nematode densities and vegetable yields.the effects of eight summer rotation crops on nematode densities and yields of subsequent spring vegetable crops were determined in field studies conducted in north florida from 1991 to 1993. the crop sequence was as follows: (i) rotation crops during summer 1991; (ii) cover crop of rye (secale cereale) during winter 1991-92; (iii) 'lemondrop l' squash (cucurbita pepo) during spring 1992; (iv) rotation crops during summer 1992; (v) rye during winter 1992-93; (vi) 'classic' eggplant (solanum melo ...199419279897
effects of tropical rotation crops on meloidogyne arenaria population densities and vegetable yields in microplots.the effects of 12 summer crop rotation treatments on population densities of meloidogyne arenaria race 1 and on yields of subsequent spring vegetable crops were determined in microplots. the crop sequence was: (i) rotation crops during summer 1991 ; (ii) cover crop of rye (secale cereale) during winter 1991-92; (iii) squash (cucurbita pepo) during spring 1992; (iv) rotation crops during summer 1992; (v) rye during winter 1992-93; (vi) eggplant (solanum melongena) during spring 1993. the 12 rotat ...199419279880
in vitro effect of vegetable and fruit juices on the mutagenicity of 2-amino-3-methylimidazo[4,5-f]quinoline, 2-amino-3,4-dimethylimidazo[4,5-f]quinoline and 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline.the antimutagenic potencies of the juices of 28 fruits and 34 vegetables commonly consumed in germany were investigated with respect to the mutagenic activities induced by 2-amino-3-methyl[4,5-f]-quinoline (iq), and in part by 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (meiq) or 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (meiqx) in salmonella typhimurium ta98 and ta100. with iq, weak to strong antimutagenic activities were found in 68% of the fruits and 73% of the vegetables that were tested ...19948206443
the control of polyphenol oxidase activity in fruits and vegetables. a study of the interactions between the chemical compounds used and heat treatment.objective of this research was to find alternative methods for the control of polyphenol oxidase (ppo) activity in fruits and vegetables with the purpose of reducing or eliminating the use of so2 for this purpose. interactions between the use of ascorbic acid, citric acid, edta, sodium metabisulphite and heat treatment (70 degrees c for 2 min) in the control of ppo activity were studied in avocado (var. fortuna), banana (var. nanica), apple (var. ana, fuji, gala & golden), pear (var. d'agua), pe ...19957659702
asymmetric somatic hybrid plants between an interspecific lycopersicon hybrid and solanum melongena.asymmetric somatic hybrid plants were obtained by a modified peg/dmso fusion procedure between protoplasts derived from suspension cells of an interspecific tomato hybrid, lycopersicon esculentum x l. pennellii, and mesophyll protoplasts of solanum melongena, eggplant. the tomato hybrid was previously transformed with agrobacterium tumefaciens and contained the kanamycin-resistance marker gene. prior to fusion, the donor protoplasts of the tomato hybrid were gamma irradiated at 9.0 krad. thus, n ...199524194315
effect of tropical rotation crops on meloidogyne incognita and other plant-parasitic nematodes.in a field experiment conducted on sandy soil in florida during the 1993 season, rotation crops of castor (ricinus communis), velvetbean (mucuna deeringina), 'mississippi silver' cowpea (vigna unguiculata), american jointvetch (aeschynomene americana), 'dehapine 51' cotton (gossypium hirsutum), and 'sx-17' sorghum-sudangrass (sorghum bicolor x s. sudanense) were effective in maintaining low population densities (<12/100 cm(3) soil) of meloidogyne incognita race 1, whereas high population densiti ...199519277319
a plant metabolic activation system from persea americana with cytochrome p450-dependent and peroxidase activities.microsomal fractions from different tissues of various plants (potato, cauliflower, aubergine, avocado pear, courgette, cucumber, banana, kiwi and strawberry) were prepared and their content of cytochrome p-450 (cyt-p450) determined. a s117 fraction from persea americana (avocado pear) presented the highest content of cyt-p450. as a consequence of these data, we have developed and characterized this fraction as a plant metabolic activation system. the p. americana s117, used in this work, contai ...19957770073
defining cooking salt intakes for patient counselling and policy making.the role of salt (nacl) in the development of high blood pressure has been a matter of debate, however, the intersalt study showed that sodium (na) intake in various areas of the world is related to the slope of blood pressure with age. accurate amounts of the total salt intake or that coming from a particular source are needed, both, for physicians who need to consider the salt intake of their patients and for public health workers who are in charge of the implementation of public health progra ...19959230039
multi-residue matrix solid-phase dispersion method for the determination of six synthetic pyrethroids in vegetables followed by gas chromatography with electron capture detection.an effective multi-residue matrix solid-phase dispersion (mspd) extraction and gas chromatographic-electron-capture detection method for the determination of six synthetic pyrethroids (fenpropathrin, cyhalothrin, permethrin, cypermethrin, fenvalerate and deltamethrin) in 5 g of vegetables (west indian gherkin, eggplant, pak-choi, cabbage and garden peas) is described. the method uses a florisil-based mspd column for direct in-line clean-up with n-hexane-acetone (9:1). recoveries calculated at 0. ...19957735459
agrobacterium mediated genetic transformation and plant regeneration via organogenesis and somatic embryogenesis from cotyledon leaves in eggplant (solanum melongena l. cv. 'kecskeméti lila').novel and efficient protocols for plant regeneration and genetic transformation from longitudinally-halved cotyledons ofin vitro raised seedlings in eggplant (solanum melongena l.) are described. after co-cultivation withagrobacterium vectors harboring neomycin phosphotransferase (nptll) as selectable marker, transgenic plantlets were regenerated on selective media containing 100 mg/l kanamycin. transformants were recovered from embryogenic calli induced by 4 mg/lα-naphthaleneacetic acid (naa), ...199524185660
cytological events during the initiation of meristematic nodules in calli derived from eggplant protoplasts.histological and ultrastructural studies have been undertaken in order to identify the particularities and, if possible, some mechanisms involved in regeneration from protoplasts in the eggplant solanum melongena. the most important result consists of a peculiar and concomitant association of meristematic and differentiated features only in cells from which regenerative processes originate.19958882523
visible spectrophotometric assay, purification, and molecular properties of a lipoxygenase from eggplant (solanum melongena linne) fruits.we purified a lipoxygenase to homogeneity from eggplant (solanum melongena linne) fruits employing the established visible spectrophotometric assay, in which the products of the lipoxygenase-catalyzed reaction, hydroperoxyfatty acids, oxidizes 2,2'-azino-di-[3-ethylbenzothiazoline-(6)-sulfonic acid] diammonium salt in the presence of a catalyst cytochrome c to yield chromophores in the visible region. this allowed for the rapid detection of the lipoxygenase activity and was helpful in accomplish ...19957575511
differential display identifies developmentally regulated genes during somatic embryogenesis in eggplant (solanum melongena l.).changes in gene expression patterns during early somatic embryogenesis of eggplant (solanum melongena l.) were characterized by means of differential display. eight products of polymerase chain reaction (pcr) derived from newly expressed mrnas after four days of culture were identified and cloned. one clone, ptm002, was found to contain a 416 bp insert and identical to the proximal arbitrary primer at both ends. based on the terminal sequences, reverse-transcription pcr (rt-pcr) was carried out ...19957544119
random amplified polymorphic dna variation in the eggplant, solanum melongena l. (solanaceae).rapd analysis was carried out on 52 accessions of solanum melongena (eggplant) and related weedy forms known as "insanum". twenty-two primers amplified 130 fragments. solanum melongena exhibited 117 of the fragments, all of which were also present in insanum. insanum displayed an additional 13 fragments not found in s. melongena. overall, the insanum accessions were more diverse than those of s. melongena. the calculated similarity between them was 0.947. the rapd results were closely concordant ...199524172917
allozyme variation in the eggplant, solanum melongena l. (solanaceae).enzyme electrophoretic studies were made in cultivated solanum melongena l. (eggplant) and similar wild and weedy forms, several of which have been thought to be different species/taxa. twenty-nine accessions of s. melongena, 33 accessions of weedy forms (referred to as "insanum") and 2 accessions of wild forms (referred to as "incanum") were surveyed for 29 isozyme loci. in s. melongena, 22 of the 29 loci were monomorphic, and nearly all of its genes were either also monomorphic or in similar f ...199524173954
effect of grafting on solasodine content in solanum viarum.solasodine--a steroid present in the mucilaginous exotesta of the seed in solanum viarum dunal (syn. s. khasianum var. chatterjeeanum) is a major source of steroidal raw material. evidence for the site of solasodine synthesis was sought through reciprocal grafts involving steroid-bearing s. viarum (both diploid and autotetraploid) and steroid-free, edible solanum melongena l. (brinjal). the results indicate that the synthesis of solasodine is scion-specific.199617252470
aubergine enhances oskar translation in the drosophila ovary.although translational regulation of maternal mrna is important for proper development of the drosophila embryo, few genes involved in this process have been identified. in this report, we describe the role of aubergine in oskar translation. previously, aubergine has been implicated in dorsoventral patterning, as eggs from aubergine mutant mothers are ventralized and seldom fertilized (schupbach, t. and wieschaus, e. (1991) genetics 129, 1119-1136). we have isolated two new alleles of aubergine ...19968625849
callus induction and plantlet formation through culture of isolated microspores of eggplant (solanum melongena l.).morphogenic calli were obtained efficiently from ab initio cultures of isolated microspores in eggplant. initial culture of freshly isolated microspores in sucrose-free medium at high temperature (35°c) for 3 d was a prerequisite for callus induction. the microspores were re-cultured in modified nln medium containing 2% sucrose and phytohormones (naa 0.5 mg l(-1), ba 0.5 mg l(-1)) in the dark. after 4 weeks of re-culture, small calli derived from microspores were transferred to ms medium contain ...199624178415
evidence for separate udp-glucose: spirostanol and udp-glucose: solasodine glucosyltransferases in solanum melongena. 19968957283
purification and properties of udp-glucose: diosgenin/tigogenin glucosyltransferase from solanum melongena leaves. 19968957282
the role of irradiation dose and dna content of somatic hybrid calli in producing asymmetric plants between an interspecific tomato hybrid and eggplant.highly asymmetric somatic hybrid plants were obtained by peg/dmso fusion of gamma-irradiated mesophyll protoplasts of the kanamycin-resistant (kmr(+)) interspecific hybrid lycopersicon esculentum x l. pennellii (ep) with mesophyll protoplasts of solanum melongena (eggplant, e). elimination of the ep chromosomes was obtained by irradiating the donor genome with different doses of gamma rays (100, 250, 500, 750 and 1000 gy). the selection of somatic hybrid calli was based on kanamycin resistance; ...199624166550
nutrient composition of the leaves and flowers of colocasia esculenta and the fruits of solanum melongena.the nutrient composition, of colocasia esculenta flowers (cf) and leaves (cl), and the green fruits of solanum melongena (sm) were carried out as a means to determine their nutritional potential. results showed that these food materials had high moisture and fiber levels which ranged between 888 and 906 g.kg-1; and 204 and 303 g.kg-1 dry weight (dw) for moisture and fiber respectively. the calorific values were between 3889 and 4001 kcals.kg-1 dw, while the total lipids ranged from 53 in cf to 7 ...19968811722
antimutagenic activity of extracts from japanese eggplant.using the salmonella/microsome assay, the antimutagenic effects of specific components of the extracts from eggplant fruits were investigated. the eggplant fruit juice exhibited an antimutagenic activity against 3-amino-1-methyl-5h-pyrido[4,3-b]indole (trp-p-2) induced mutagenicity. in some of the fractions extracted with several organic solvents (acetone, petroleum ether, ethyl acetate; and methanol), the activity was recognized. no mutagenicity or toxicity for salmonella typhimurium ta98 in th ...19968950351
specific sequence modifications of a cry3b endotoxin gene result in high levels of expression and insect resistance.solanum melongena (eggplant) cv. picentia and the wild species solanum integrifolium were transformed with both a wild type (wt) and four mutagenized versions of bacillus thuringiensis (bt) gene bt43 belonging to the cry3 class. the bt gene was partly modified in its nucleotide sequence by replacing four target regions (w: +1 to +170; x: +592 to +1057; y: +1203 to +1376; z: +1376 to +1984) with synthetic fragments obtained by polymerase chain reaction amplification of crude oligonucleotides. the ...19979225859
the effects of calystegines isolated from edible fruits and vegetables on mammalian liver glycosidases.the polyhydroxylated nortropane alkaloids called calystegines occur in many plants of the convolvulaceae, solanaceae, and moraceae families. certain of these alkaloids exhibit potent inhibitory activities against glycosidases and the recently demonstrated occurrence of calystegines in the leaves, skins, and sprouts of potatoes (solanum tuberosum), and in the leaves of the eggplant (s. melongena), has raised concerns regarding the safety of these vegetables in the human diet. we have surveyed the ...19979455909
genetic engineering of parthenocarpic plants.transgenic tobacco and eggplants expressing the coding region of the iaam gene from pseudomonas syringae pv. savastanoi, under the control of the regulatory sequences of the ovule-specific defh9 gene from antirrhinum majus, showed parthenocarpic fruit development. expression of the defh9-iaam chimeric transgene occurs during flower development in both tobacco and eggplant. seedless fruits were produced by emasculated flowers. when pollinated, the parthenocarpic plants produced fruits containing ...19979415894
new satellite rnas, but no di rnas, are found in natural populations of tomato bushy stunt tombusvirus.a collection of 57 field isolates of the tombusvirus tomato bushy stunt virus was obtained from eggplant and tomato during 1994-1997 and was examined for the presence of defective interfering (di) rna species by northern blot hybridization and rt-pcr. no di rna species were detected associated with any of the field tbsv isolates. however, serial passaging of two field isolates in nicotiana clevelandii at high multiplicity of infection resulted in the rapid generation of di-like rna species, indi ...19979434719
hypolipidemic effect of flavonoids from solanum melongena.flavonoids extracted from the fruits of solanum melongena (brinjal) orally administered at a dose of 1 mg/100 g bw/day showed significant hypolipidemic action in normal and cholesterol fed rats. hmg coa reductase activity was found to be enhanced, while activities of glucose-6-phosphate dehydrogenase and malate dehydrogenase were significantly reduced. activities of lipoprotein lipase and plasma lcat showed significant enhancement. a significant increase in the concentrations of hepatic and feca ...19979650725
inhibition of the mutagenicity of 2-nitrofluorene, 3-nitrofluoranthene and 1-nitropyrene by vitamins, porphyrins and related compounds, and vegetable and fruit juices and solvent extracts.when 21 vitamins including related compounds haemin, chlorophyllin, chlorophyll, biliverdin and bilirubin, as well as juices from five fruits and 25 vegetables and solvent extracts from the residues of fruits and vegetables were tested for their antimutagenic potencies with respect to mutagenicity induced by 2-nitrofluorene (2-nf), 3-nitrofluoranthene (3-nfa) and 1-nitropyrene(1-np) in salmonella typhimurium ta98 the following results were obtained. the tetracyclic nitroarenes 3-nfa and 1-np wer ...19979207899
an exo-poly-alpha-d-galacturonosidase, pehb, is required for wild-type virulence of ralstonia solanacearum.ralstonia solanacearum, which causes bacterial wilt disease of many plant species, produces several extracellular plant cell wall-degrading enzymes that are suspected virulence factors. these include a previously described endopolygalacturonase (pg), peha, and two exo-pgs. a gene encoding one of the exo-pgs, pehb, was cloned from r. solanacearum k60. the dna fragment specifying pehb contained a 2,103-bp open reading frame that encodes a protein of 74.2 kda with a typical n-terminal signal sequen ...19979393701
jasmonic acid inducible aspartic proteinase inhibitors from potato.a new cdna clone coding for an aspartic proteinase inhibitor homologue was isolated from a potato tuber cdna library. southern blot analysis was used to study the structural diversity of the aspartic proteinase inhibitor gene family in several species of the solanaceae. the existence of sequence-homologous genes was confirmed in the genomic dna of different potato cultivars (solanum tuberosum l. cv. désirée, pentland squire and igor), tomato (lycopersicon esculentum mill.), aubergine (s. melonge ...19979055446
udp-glucose:solasodine glucosyltransferase from eggplant (solanum melongena l.) leaves: partial purification and characterization.uridine 5'-diphosphoglucose-dependent glucosyltransferase which catalyzes the glycosylation of solasodine i.e. udp-glucose:solasodine glucosyltransferase, is present in leaves, roots, unripe fruits and unripe seeds of eggplant (solanum melongena l.). the glucosylation product is chromatographically identical with authentic solasodine 3 beta-d-monoglucoside, a putative intermediate in the biosynthesis of solasodine-based glycoalkaloids characteristic of the eggplant. the enzyme was purified about ...19979241353
temporal regulation of somatic embryogenesis by adjusting cellular polyamine content in eggplantfour critical stages of embryogenesis, including callus induction, cellular acquisition of morphogenetic competence, expression of embryogenic program, and development and maturation of somatic embryos during somatic embryogenesis from leaf discs of eggplant (solanum melongena l.), were identified by scanning electron microscopy. temporal changes in arginine decarboxylase (adc) activity and polyamines (pas) during critical stages of embryogenesis revealed that high levels of pas (especially putr ...19989490762
comparative bio-antimutagenicity of common vegetables and traditional vegetables in kyoto.traditional vegetables in kyoto are a unique group of vegetables that have been cultivated in limited areas near kyoto city. we compared the traditional vegetables in kyoto with common vegetables for the bio-antimutagenicity of their extracts against uv-induced mutation of e. coli b/r wp2. among the traditional vegetables in kyoto, kamo eggplant (solanaceae) and katsura oriental pickling melon (cucurbitaceae) showed higher bio-antimutagenicity and yield in the n-hexane, chloroform and ethyl acet ...19989692200
a large outbreak of botulism: the hazardous baked potato.in april 1994, the largest outbreak of botulism in the united states since 1978 occurred in el paso, texas. thirty persons were affected; 4 required mechanical ventilation. all ate food from a greek restaurant. the attack rate among persons who ate a potato-based dip was 86% (19/22) compared with 6% (11/176) among persons who did not eat the dip (relative risk [rr] = 13.8; 95% confidence interval [ci], 7.6-25.1). the attack rate among persons who ate an eggplant-based dip was 67% (6/9) compared ...19989652437
[effect of eggplant on plasma lipid levels, lipidic peroxidation and reversion of endothelial dysfunction in experimental hypercholesterolemia].to study the effect of egg plant on endothelium-dependent relaxation, and plasma lipids in hypercholesterolemic rabbits, and to assess influence of this plant on the malondialdehyde (mda) content of ldl particles and the arterial wall.19989659714
evidence for transovarial transmission of tomato yellow leaf curl virus by its vector, the whitefly bemisia tabaci.the whitefly bemisia tabaci is the only vector of the tomato yellow leaf curl geminivirus (tylcv). the insect transmits the virus in a persistent-circulative manner. tylcv dna was detected by polymerase chain reaction and by southern blot hybridization in progeny (eggs, first and second instars, adults) of single viruliferous whiteflies that developed on eggplant or on cotton (two tylcv nonhost plants). furthermore, tylcv dna was present in the progeny of insects that had acquired the virus thro ...19989454703
inhibitory effect of delphinidin from solanum melongena on human fibrosarcoma ht-1080 invasiveness in vitro.we investigated the inhibitory effect of eggplant (solanum melongena var. marunasu) extract on human fibrosarcoma ht-1080 cell invasion of reconstituted basement membrane [matrigel (mg)]. we found that the effective component of the plant extract was delphinidin, a flavonoid pigment contained in the peel. the extract and delphinidin did not affect tumor cell adhesion to mg or haptotactic migration to mg. ht-1080 secretes matrix metalloproteinase(mmp)-2 and mmp-9, which degrade extracellular matr ...19989581517
transfer rna mimicry among tymoviral genomic rnas ranges from highly efficient to vestigial.three trna-associated properties of a representative set of tymoviral rnas have been quantitatively assessed using higher plant (wheat germ) proteins: aminoacylation, ef-1alpha*gtp binding, and 3'-adenylation of 3'-cc forms of the rnas by ctp, atp:trna nucleotidyltransferase. the rnas fall into three classes differing in the extent of trna mimicry. turnip yellow mosaic (tymv) and kennedya yellow mosaic virus rnas had activities in all three properties similar to those of a higher plant trnavaltr ...19989742235
ralstonia solanacearum pectin methylesterase is required for growth on methylated pectin but not for bacterial wilt virulenceralstonia (pseudomonas) solanacearum causes bacterial wilt, a serious disease of many crop plants. the pathogen produces several extracellular plant cell wall-degrading enzymes, including polygalacturonases (pgs) and pectin methylesterase (pme). pme removes methyl groups from pectin, thereby facilitating subsequent breakdown of this cell wall component by pgs, which are known bacterial wilt virulence factors. r. solanacearum pgs could not degrade 93% methylated pectin unless the substrate was fi ...19989835583
bioactive phytochemicals with emphasis on dietary practices.diet can modify the pathophysiological processes of various metabolic disorders and can be an effective preventive strategy for various disease processes most of which are known to involve oxidative damage. both nutrient and non-nutrient components of the diet have been recognized for their anti-oxidant and other potential benefits. plant foods contain phytochemicals such as flavonoids, phenolic acids, etc., which show biological activity. some common foods used in indian culinary practices were ...19989863273
antioxidant activity of nasunin, an anthocyanin in eggplant.delphinidine-3-(p-coumaroylrutinoside)-5-glucoside (nasunin), an anthocyanin was isolated as purple colored crystals from eggplant peels, solanum melongena l. 'chouja'. using an electron spin resonance spectrometer and 5,5-dimethyl-1-pyrroline-n-oxide (dmpo), spin trapping, hydroxyl (.oh) or superoxide anion radicals (02*-) generated by the fenton reaction or the hypoxanthine-xanthine oxidase system were measured as dmpo-oh or dmpo-ooh spin adducts. l-ascorbic acid 2-[3,4-dihydro-2,5,7,8-tetra-m ...199810100509
developmental toxicology of solamargine and solasonine glycoalkaloids in frog embryos.as part of an effort to improve the safety of plant foods, a need exists to define the relative toxicities of structurally different glycoalkaloids and metabolites which may be present in solanum plant species such as potatoes, tomatoes and eggplants. the objectives of this study were to determine the relative toxicities and the modes of action of the eggplant (solanum melongena) glycoalkaloids solamargine and solasonine in xenopus laevis frog embryos, using membrane potential and embryo growth ...19989662413
the effect of waste water reuse in irrigation on the contamination level of food crops by giardia cysts and ascaris eggs.in marrakech, raw sewage has been used for farming purposes for several decades for many types of crops. this study aimed to determine the contamination level of giardia cysts and ascaris eggs for crops designated for human consumption. collected crops in irrigated fields were turnip, marrow, squash, potatoes, pepper and eggplant. field trials were also carried out on four crops, coriander, carrots, mint and radish, using three water types for irrigation, i.e. raw waste water, treated waste wate ...199910477066
antioxidant activity of flavonoids from solanum melongena.flavonoids isolated from solanum melongena (brinjal) showed potent antioxidant activity. concentrations of malondialdehyde, hydroperoxides and conjugated dienes were lowered significantly. the activity of catalase was found to be significantly enhanced in the tissues of normal and cholesterol fed rats administered 1 mg flavonoid from brinjal/100 g bw/day. the concentration of glutathione also showed elevated values in the experimental animals. the elevated levels of glutathione and significantly ...199910441778
influence of sodium chloride concentration on the controlled lactic acid fermentation of "almagro" eggplants.the effect of a commercial lactobacillus starter and sodium chloride concentration on the fermentation of "almagro" eggplants (solanum melongena l. var. esculentum depressum) was studied. the results of fermentation using added starter and varying salt concentrations (4, 6, and 10% w/v) in brine were compared with the results of spontaneous fermentation taking place in brine with a salt concentration of 4%. fresh fruits, medium in size (34-44 g), were used in all cases; all fruits were blanched ...199910598110
expression of cysteine proteinase during developmental events associated with programmed cell death in brinjal.here we show that the expression of a cysteine proteinase coincides with several developmental events associated with programmed cell death (pcd) in solanum melongena (brinjal), i.e. during leaf senescence, fruit senescence, xylogenesis, nucellar cell degeneration and anther senescence. we have isolated a cdna encoding brinjal cysteine proteinase (smcp) that shares high (90-92%) amino acid identity to cysteine proteinases of tobacco (cyp-8) and tomato (lcyp-2) that have not been previously repor ...199910097390
host range specificity in verticillium dahliae.abstract verticillium dahliae isolates from artichoke, bell pepper, cabbage, cauliflower, chili pepper, cotton, eggplant, lettuce, mint, potato, strawberry, tomato, and watermelon and v. albo-atrum from alfalfa were evaluated for their pathogenicity on all 14 hosts. one-month-old seedlings were inoculated with a spore suspension of about 10(7) conidia per ml using a root-dip technique and incubated in the greenhouse. disease incidence and severity, plant height, and root and shoot dry weights we ...199918944648
development of a simple sample preparation technique for gas chromatographic-mass spectrometric determination of nicotine in edible nightshades (solanaceae).the purpose of this study was to develop a rapid analytical method for the reliable determination of low concentrations of nicotine in foods for large numbers of samples. food material was extracted using a simple liquid-liquid extraction method. for processed foods, further clean-up steps had to be employed to eliminate interfering compounds. the determination of nicotine was performed by gas chromatography-mass spectrometry. quantitative analysis was accomplished using deuterium labeled nicoti ...199910343400
determination of the nicotine content of various edible nightshades (solanaceae) and their products and estimation of the associated dietary nicotine intake.this investigation was initiated as a result of proposals in the literature that dietary nicotine intake could contribute to the level of nicotine metabolites in biological fluids such as salivary cotinine concentration. nicotine concentration was determined in several frequently consumed vegetables from the nightshade family (solanaceae) (i.e., tomatoes, potatoes, aubergines, and peppers), as well as in some of their processed products. the edible solanaceae fruit analyzed in this investigation ...199910552617
changes in polyamines and ethylene during the development and ripening of eggplant fruits (solanum melongena).the levels of free polyamines in the endocarpium of eggplants (solanum melongena) cv. black nite were studied during fruit growth and ripening. other parameters such as weight and volume variation, respiratory intensity, ethylene production, and sugar content were determined. the polyamines found were putrescine and spermidine, with a higher amount of the former. no spermine, agmatine, or cadaverine were found during the development and ripening period of eggplant. at the beginning of fruit deve ...199910563994
[population growth, distribution pattern and sampling technique of thrips palmi on eggplant].population growth of thrips palmi karny on eggplant (solanum melongena l.) were analyzed by mathematical models. the rate of population increase (r) of the adults and immature (instars 1-2) t. palmi were estimated to be 0.0630 and 0.0801 respectively in the open fields, and 0.0983 and 0.1036 respectively in the greenhouse. k value of logistic curve of t. palmi was estimated to be 33.90 in the greenhouse, and 23.50 in the open fields. the relationship between mean crowding (m*) and mean density ( ...200011767560
[transmission of stolbur virus of pepper and aubergines by grafting and the leaf-hopper hyalesthes obsoletus sign]. 20005619957
chayote mosaic virus, a new tymovirus infecting cucurbitaceae.abstract chayote mosaic virus (chmv) is a putative tymovirus isolated from chayote crops in costa rica. chmv was characterized at the host range, serological, and molecular levels. chmv was transmitted mechanically and induced disease symptoms mainly in cucurbitaceae hosts. asymptomatic infections were detected in other host families. serologically, chmv is related to the andean potato latent virus (aplv) and the eggplant mosaic virus (emv), both members of the genus tymovirus infecting solanace ...200018944472
vegetative compatibility groups of verticillium dahliae in israel: their distribution and association with pathogenicity.a collection of 565 isolates of verticillium dahliae, recovered between 1992 and 1997 from 13 host plant species and soil at 47 sites in israel, was tested for vegetative compatibility using nitrate-nonutilizing (nit) mutants. three vegetative compatibility groups (vcgs) were found and identified as vcg2a (28 isolates), vcg2b (158 isolates), and vcg4b (378 isolates) by using international reference strains. one isolate was heterokaryon self-incompatible. of the vcg2b isolates, 92% were recovered ...200018944560
ralstonia solanacearum ampd is required for wild-type bacterial wilt virulence.abstract a gene resembling enterobacterial ampd was identified in the bacterial wilt pathogen, ralstonia solanacearum. the gene lies 13 bp 3' of pehsr, a two-component positive regulator of virulence factors such as plant cell wall-degrading polygalacturonases and bacterial motility. ampd, an n-acetylmuramyl-l-alanine amidase, degrades and recycles bacterial cell wall components and also plays a role in the induction of beta-lactamase, which confers ampicillin resistance. ampd is probably not in ...200020572964
aiia, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of erwinia carotovora.n-acylhomoserine lactones, known as autoinducers (ais), are widely conserved signal molecules present in quorum-sensing systems of many gram-negative bacteria. ais are involved in the regulation of diverse biological functions, including expression of pathogenic genes in the plant pathogens pseudomonas solanacearum, several erwinia species, and the human pathogen pseudomonas aeruginosa. a bacterial isolate, bacillus sp. 240b1, is capable of enzymatic inactivation of ais. the gene (aiia) for ai i ...200010716724
development and evaluation of a model predicting the survival of escherichia coli o157:h7 nctc 12900 in homemade eggplant salad at various temperatures, phs, and oregano essential oil concentrations.homemade eggplant salad, a traditional greek appetizer, was inoculated with escherichia coli o157:h7 nctc 12900 supplemented with different concentrations of oregano essential oil (0.0, 0.7, 1. 4, and 2.1% [vol/wt]) and stored at different temperatures (0, 5, 10, and 15 degrees c). the product's ph was adjusted to 4.0, 4.5, or 5. 0 with lemon juice. for each combination of the environmental factors, the bacterial counts were modeled, using the baranyi model, as a function of time to estimate the ...200010742255
antioxidant activity of nasunin, an anthocyanin in eggplant peels.the free radical scavenging activities and inhibitory effect of lipid peroxidation of a delphinidin derivative in eggplant were investigated. delphinidin-3-(p-coumaroylrutinoside)-5-glucoside (nasunin), an anthocyanin, was isolated as purple colored crystals from eggplant peels. using electron spin resonance spectrometry and 5,5-dimethyl-1-pyrroline-n-oxide (dmpo), hydroxyl radicals (oh) or superoxide anion radicals (o(2)(-)) generated by the fenton reaction or the hypoxanthine-xanthine oxidase ...200010962130
eggplant (solanum melongena) infusion has a modest and transitory effect on hypercholesterolemic subjects.eggplant (solanum melongena) is consumed extensively in brazil. it has been believed that infusion of a powdered preparation of the fruit may reduce serum cholesterol. however, there are few documented reports on its effects on cholesterol metabolism and its possible hypocholesterolemic effect has not been proved by well-controlled studies. the aim of the present study was to observe the effects of s. melongena on the serum cholesterol and triglycerides of 38 hypercholesterolemic human volunteer ...200010973133
prominent artificial radionuclide activity in the environment of coastal karnataka on the southwest coast of india.studies on radiation level and radionuclide distribution in the environment of coastal karnataka were undertaken to provide baseline data for the future assessment of the impact of the nuclear and thermal power stations that are being set up in the region and to understand the behaviour of radionuclides in the environment. as part of the programme the concentrations of two important artificial radionuclides, namely 90sr and 137cs, have been measured in a number of environmental samples. the conc ...200011008934
occurrence and significance of cryptococcus neoformans in vegetables and fruits.cryptococcus neoformans was isolated from three of 437 (0.6%) samples of a wide variety of vegetables collected from a number of markets in delhi. the vegetables yielding c. neoformans were tomato, lycopersicon esculentum (1/70), 'vegetable sponge', luffa cylindrica (1/56) and brinjal, solanum melongena (1/55). only solitary samples of these three vegetables were found to be positive. in vitro studies at 25 degrees c revealed that a reference c. neoformans isolate readily colonized, as did candi ...200015597680
plant hydraulic conductance measured by the high pressure flow meter in crop plants.a new high pressure flow meter (hpfm) method for measuring plant hydraulic conductances (k) was investigated to examine whether its results are comparable to those from a conventional evaporative flux (ef) method in crops. hydraulic conductance (k) was measured by the two methods under quasi-steady-state conditions in six crops grown in pots: soybean (glycine max l. merr. cv. tsurunoko daizu), sunflower (helianthus annuus l. cv. russian mammoth), kidney bean (phaseolus vulgaris l. cv. tsurunashi ...200010938875
comparative effectiveness of an integrated pest management system and other control tactics for managing the spider mite tetranychus ludeni (acari: tetranychidae) on eggplant.the effect of an integrated pest management (ipm) package, host plant resistance, chrysoperla carnea predation and neem oil were evaluated against the spider mite tetranychus ludeni on eggplant (solanum melongena l.) fields in 1996 and 1997, by estimating the mite population density and yield levels. compared with the ipm package (panruti local, c. carnea plus neem oil), the standard (susceptible) eggplant variety (mdu1) grown by farmers and treated with an acaricide (dicofol) had significantly ...200112465852
alpha-tocopherol content in 62 edible tropical plants.vitamin e was determined by the high-performance liquid chromatography (hplc) method. all the plants tested showed differences in their alpha-tocopherol content and the differences were significant (p < 0.05). the highest alpha-tocopherol content was in sauropus androgynus leaves (426.8 mg/kg edible portion), followed by citrus hystrix leaves (398.3 mg/kg), calamus scipronum (193.8 mg/kg), starfruit leaves averrhoa belimbi (168.3 mg/kg), red pepper capsicum annum (155.4 mg/kg), local celery apiu ...200111410015
phytophthora infestans enters the genomics era.summary phytophthora infestans, cause of late-blight, is the most devastating disease of potato world-wide. recent years have seen a dramatic intensification in molecular biological studies of p. infestans, including the development of novel tools for transformation and gene silencing and the resources for genetical, transcriptional and physical mapping of the genome. this review will focus on the increasing efforts to use these resources to discover the genetic bases of pathogenicity, avirulenc ...200120573013
genetic diversity of japanese strains of ralstonia solanacearum.abstract the genetic diversity of 74 japanese strains of ralstonia solanacearum was assessed by pathogenicity tests and the repetitive sequencebased polymerase chain reaction (rep-pcr) fingerprint method. based on their genomic fingerprints, biovar n2 strains were divided into two distinct groups, one consisting of potato isolates belonging to race 3, and the other consisting of tomato, eggplant, pepper, and tobacco isolates belonging to race 1. biovar 3 strains had low average similarity and we ...200118943853
pathogenic and nonpathogenic lifestyles in colletotrichum acutatum from strawberry and other plants.abstract anthracnose is one of the major fungal diseases of strawberry occurring worldwide. in israel, the disease is caused primarily by the species colletotrichum acutatum. the pathogen causes black spot on fruit, root necrosis, and crown rot resulting in mortality of transplants in the field. the host range and specificity of c. acutatum from strawberry was examined on pepper, eggplant, tomato, bean, and strawberry under greenhouse conditions. the fungus was recovered from all plant species o ...200118944126
source of resistance against ralstonia solanacearum in fertile somatic hybrids of eggplant (solanum melongena l.) with solanum aethiopicum l.solanum aethiopicum is reported to carry resistance to bacterial wilt disease caused by ralstonia solanacearum, which is one of the most important diseases of eggplant (solanum melongena). these two species can sexually be crossed but the fertility of their progeny is very low. in order to transfer the resistance and improve the fertility, somatic hybrids between s. melongena cv. dourga and two groups of s. aethiopicum were produced by electrical fusion of mesophyll protoplasts. thirty hybrid pl ...200111164602
inhibition of immunologic and nonimmunologic stimulation-mediated anaphylactic reactions by water extract of white eggplant (solanum melongena).we investigated the effect of water extract of solanum melongena(smwe) on immunologic and nonimmunologic stimulation-mediated anaphylactic reactions. nonimmunologic anaphylactic reaction was induced by compound 48/80 injection. oral administration of smwe (1 g kg(-1)) completely inhibited compound 48/80-induced anaphylactic reaction. immunologic anaphylactic reaction was generated by sensitizing the skin with anti-dinitrophenyl (dnp) ige followed 48 h later with an injection of antigen. oral adm ...200111352546
bioactive anthocyanins detected in human urine after ingestion of blackcurrant juice.anthocyanins are a group of very efficient bioactive compounds that are widely distributed in plant food. several fruits (blackcurrant, blackberry, red grape) and some vegetables (eggplant, onion, red radish) are rich sources of these natural pigments. extracts of some of them are used as food colorants as well as components of pharmaceutical preparations and functional foods. anthocyanins, through their ability to inhibit radical reactions, are considered to exert several protective effects in ...200111394716
aubergine encodes a drosophila polar granule component required for pole cell formation and related to eif2c.in drosophila oocytes, activation of oskar translation from a transcript localized to the posterior pole is an essential step in the organization of the pole plasm, specialized cytoplasm that contains germline and abdominal body patterning determinants. oskar is a component of polar granules, large particles associated with the pole plasm and the germline precursor pole cells of the embryo. aubergine mutants fail to translate oskar mrna efficiently and are thus defective in posterior body patter ...200111526087
chemical composition and biochemical properties of mirlitons (sechium edule) and purple, green, and white eggplants (solanum melongena). 2001701618
hyperhydricity in in vitro eggplant regenerated plants: structural characteristics and involvement of bip (binding protein).the hyperhydricity in eggplant (solanum melongena l.) plants was monitored by the induction of the er-luminal resident protein bip. although tissue culture conditions may induce bip synthesis, the accumulation of bip in hyperhydric shoots was consistently higher than in non-hyperhydric shoots. the leaf and stem anatomy in non-hyperhydric and hyperhydric eggplant was investigated aiming to identify structural changes associated with this phenomenon. in non-hyperhydric organs there were smaller an ...200111297782
purification of a novel lipoxygenase from eggplant (solanum melongena) fruit chloroplasts.a novel membrane lipoxygenase (lox; ec 1.13.11.12) from eggplant (solanum melongena l. cv. belleza negra) fruit chloroplasts has been purified 20-fold to a specific activity of 207 enzymatic units per mg of protein with a yield of 72%. the purification was carried out by sonicating the chloroplastic membranes in the presence of triton x-114 followed by phase partitioning and anion exchange chromatography. the purified membrane lox preparation consisted of a single major band with an apparent mol ...200111240910
eggplant lipoxygenase (solanum melongena): product characterization and effect of physicochemical properties of linoleic acid on the enzymatic activity.lipoxygenase (lox) from eggplant (solanum melongena l. cv. belleza negra) was partially purified, and the products and kinetics of the enzyme were studied. linoleic acid (la) was the best substrate for this enzyme. product analysis by hplc and gc/ms revealed that, at its ph optimum (ph 7.0), the enzyme converted la almost totally into the 9-hydroperoxy isomer, whereas the 13-hydroperoxy isomer was only a minor product. at this ph, the enzyme had k(m) and v(max) values for la of 1.4 microm and 2. ...200111170610
rnai is activated during drosophila oocyte maturation in a manner dependent on aubergine and spindle-e.gene silencing by double-stranded rna is a widespread phenomenon called rnai, involving homology-dependent degradation of mrnas. here we show that rnai is established in the drosophila female germ line. mrna transcripts are translationally quiescent at the arrested oocyte stage and are insensitive to rnai. upon oocyte maturation, transcripts that are translated become sensitive to degradation while untranslated transcripts remain resistant. mutations in aubergine and spindle-e, members of the pi ...200212154120
evaluating the safety of beta-cyfluthrin insecticide for usage in eggplant (solanum melongena l.) crop. 200211993816
a comparative genetic linkage map of eggplant (solanum melongena) and its implications for genome evolution in the solanaceae.a molecular genetic linkage map based on tomato cdna, genomic dna, and est markers was constructed for eggplant, solanum melongena. the map consists of 12 linkage groups, spans 1480 cm, and contains 233 markers. comparison of the eggplant and tomato maps revealed conservation of large tracts of colinear markers, a common feature of genome evolution in the solanaceae and other plant families. overall, eggplant and tomato were differentiated by 28 rearrangements, which could be explained by 23 par ...200212196412
genomic fingerprinting of virulent and avirulent strains of clavibacter michiganensis subspecies sepedonicus.genomic fingerprints of c. michiganensis subsp. sepedonicus were generated by chef gel electrophoresis of restriction digested high-molecular weight dna. low levels of intra-subspecific variation were detected by cluster analysis of the fingerprints. four haplotypes were identified by genomic fingerprinting with hindiii, and eight were identified with ecori. haplotypes generated with hindiii were less similar than those generated by ecori. haplotypes generated with hindiii formed groups that cor ...200211815855
oxidative degradation of bisphenol a by crude enzyme prepared from potato.when crude enzymes prepared from some vegetables and fruits were incubated with bisphenol a (2,2-bis(4-hydroxyphenyl)propane, bpa) at 37 degrees c, bpa was oxidized by crude enzymes from potato, eggplant, and lettuce. the crude enzyme prepared from potato (solanum tuberosum) had the strongest oxidative activity for bpa. its optimal temperature and ph were 40-45 degrees c and 8.0, respectively. more than 95% of bpa was oxidized after the incubation with potato enzyme for 60 min. bpa gave two oxid ...200212381152
effect of freezing on the spontaneous fermentation and sensory attributes of almagro eggplants.a study of the physico-chemical and microbiological processes taking place during the spontaneous fermentation of eggplant fruits that had been frozen after blanching revealed a delay in the onset of the active fermentation stage, which has been ascribed to the adverse effect of the freezing temperature employed on the lactic acid bacteria involved in fermentation. the species most affected by freezing were l. fermentum and l. brevis biotype 2. despite this finding, the pattern of fermentation w ...200212076033
genetically modified parthenocarpic eggplants: improved fruit productivity under both greenhouse and open field cultivation.parthenocarpy, or fruit development in the absence of fertilization, has been genetically engineered in eggplant and in other horticultural species by using the defh9-iaam gene. the iaam gene codes for tryptophan monoxygenase and confers auxin synthesis, while the defh9 controlling regions drive expression of the gene specifically in the ovules and placenta. a previous greenhouse trial for winter production of genetically engineered (gm) parthenocarpic eggplants demonstrated a significant increa ...200211934354
identification of aminoethylcysteine ketimine decarboxylated dimer, a natural antioxidant, in dietary vegetables.aminoethylcysteine ketimine decarboxylated dimer (simply named dimer) is a natural sulfur-containing tricyclic compound detected, until now, in human urine, bovine cerebellum, and human plasma. recently, the antioxidant properties of this compound have been demonstrated. in this investigation, the presence of aminoethylcysteine ketimine decarboxylated dimer was identified in garlic, spinach, tomato, asparagus, aubergine, onion, pepper, and courgette. identification of this compound in dietary ve ...200211902974
optimisation of transgene action at the post-transcriptional level: high quality parthenocarpic fruits in industrial tomatoes.genetic engineering of parthenocarpy confers to horticultural plants the ability to produce fruits under environmental conditions that curtail fruit productivity and quality. the defh9-iaam transgene, whose predicted action is to confer auxin synthesis specifically in the placenta, ovules and derived tissues, has been shown to confer parthenocarpy to several plant species (tobacco, eggplant, tomato) and varieties.200211818033
tobacco to tomatoes: a phylogenetic perspective on fruit diversity in the solanaceae.the solanaceae contains many species of agricultural importance. several of these are cultivated for their fruits, such as the tomato, the pepper and the aubergine. the family is very diverse in fruit type with capsules, drupes, pyrenes, berries, and several sorts of dehiscent non-capsular fruits occurring in the 90+ genera. in this paper, recent work on fruit type evolution in angiosperms is reviewed in relation to dispersal agents and habitat ecology. defining fruit types in the solanaceae in ...200212324525
determination of acetamiprid, imidacloprid, and nitenpyram residues in vegetables and fruits by high-performance liquid chromatography with diode-array detection.determination of 3 neonicotinoid insecticides, nitenpyram, imidacloprid, and acetamiprid, was studied. vegetables and fruits were extracted with acetonitrile. the crude extract was passed through a weak anion-exchange cartridge (psa). the effluent was subjected to silica gel cartridge. imidacloprid and acetamiprid were eluted with 10 ml of 4:6 (v/v) acetone/hexane, followed by nitenpyram with acetone (20 ml). pesticides were determined by hplc with a c-18 column and diode-array detection system. ...200212137461
effect of eggplant (solanum melongena) extract on the in vitro labeling of blood elements with technetium-99m and on the biodistribution of sodium pertechnetate in rats.the use of eggplant has been suggested to treat different diseases. we studied the effect of eggplant extract on the labeling of red blood cells (rbc) and plasma proteins with technetium-99m (tc-99m) and on biodistribution of sodium pertechnetate (tc-99m) in rats. blood was incubated with an eggplant extract (final concentrations 3.12 to 250.00 mg/ml) for 60 min. then, stannous chloride (sncl2) (0.06 or 1.2 microg/ml) and tc-99m, as sodium pertechnetate, were added. samples of rbc and plasma (p) ...200212619974
tomato yellow leaf curl virus in the dominican republic: characterization of an infectious clone, virus monitoring in whiteflies, and identification of reservoir hosts.abstract epidemics of tomato yellow leaf curl disease (tylcd) in the dominican republic in the early to mid-1990s resulted in catastrophic losses to processing tomato production. as part of an integrated management approach to tylcd, the complete nucleotide sequence of a full-length infectious clone of an isolate of tomato yellow leaf curl virus (tylcv) from the dominican republic (tylcv-[do]) was determined. the tylcv-[do] genome was nearly identical in sequence (>97%) and genome organization t ...200218943022
use of green fluorescent protein-transgenic strains to study pathogenic and nonpathogenic lifestyles in colletotrichum acutatum.abstract colletotrichum acutatum, which causes anthracnose disease on strawberry, can also persist on several other plant species without causing disease symptoms. the genetic and molecular bases that determine pathogenic and nonpathogenic lifestyles in c. acutatum are unclear. we developed a transformation system for c. acutatum by electroporation of germinating conidia, and transgenic isolates that express the green fluorescent protein (gfp) were produced. details of the pathogenic and nonpath ...200218943270
host-range characterization of two pratylenchus coffeae isolates from brazil.two isolates of pratylenchus coffeae were collected from coffee roots (in marília, são paulo state, brazil) and aglaonema (in rio de janeiro city, rio de janeiro state, brazil) and maintained in the laboratory on alfalfa callus. twenty-four plants were tested in the greenhouse to characterize the host preference of these isolates. the host ranges of the isolates differed from each other and, interestingly, coffee, banana, and citrus were not among the better hosts of either isolate. rather, sorg ...200219265921
nitrate and nitrite in vegetables from north china: content and intake.the contents of nitrate and nitrite in potato, cabbage, chinese cabbage, scallion (shallot), celery, cucumber, tomato, eggplant and wax gourd taken from the north china market from 1998 to 1999 were determined. these vegetables provide the major contribution to the nitrate intake from the diet. the highest content of nitrate was found in celery followed by chinese cabbage, cabbage, scallion, wax gourd and eggplant. for all the products, a great variation in the content of nitrate was found. gene ...200212623672
synthesis of theaflavin from epicatechin and epigallocatechin by plant homogenates and role of epicatechin quinone in the synthesis and degradation of theaflavin.oxidation products of (-)-epicatechin and (-)-epigallocatechin by treatment with homogenates of 62 plants belonging to 49 families were compared. forty-six plants were capable of synthesizing theaflavin, a black tea pigment, regardless of whether they contained catechins. loquat, japanese pear, and blueberry had activities higher than that of fresh tea leaves after 5 h of treatment; furthermore, these plants oxidized theaflavin to theanaphthoquinone. an additional new metabolite, dehydrotheasine ...200211902970
synergistic antioxidative properties of phenolics from natural origin toward low-density lipoproteins depend on the oxidation system.using an approach in line with that of a previous report, we assessed the antioxidant activity of several natural, polyphenol- or tocotrienol-rich mixtures: extracts from elaesis guineensis oil (a) and vitis vinifera (b), a coffea robusta powder (c), and extracts from olea europea mill wastewaters (d), solanum melongena (e), and lycopersicon esculentum (f). the copper- and 2-2'-azobis(2-amidinopropane) hydrochloride (aaph)-oxidation systems were used in the presence of low-density lipoprotein. f ...200212487753
proximate and mineral compositions of cooked and uncooked solanum melongena.the proximate and mineral compositions of four different varieties of cooked and uncooked solanum melongena were determined. except for the percentage moisture, the levels of crude protein, crude fat, total ash and carbohydrate content were generally higher in uncooked samples than in cooked samples. solanum macrocarpum (uncooked) has the highest moisture content with an average value of 73.9% and 84.5% for coat and flesh respectively, while the coat of solanum sessiliflorum and flesh of solanum ...200211939104
occurrence and characterization of hypovirulence in the tan sclerotial isolate s10 of sclerotinia sclerotiorum.spontaneously-occurring hypovirulence in the tan sclerotial isolate s10 of sclerotinia sclerotiorum from sunflower in canada was characterized and compared to another hypovirulent isolate ep-1pn of s. sclerotiorum from eggplant in china. hypovirulent isolates derived from s10 were purified by single hyphal tip isolations from colonies of s10 showing abnormal growth on potato dextrose agar (pda) and tested for pathogenicity on leaves of canola (brassica napus cv. 'westar'). these abnormal isolate ...200315000237
pathogenicity of phytophthora infestans on solanaceous and asteraceous plant species in cameroon.experiments were conducted to detect potential hosts of phytophthora infestans, causal agent of potato late blight. isolates of p. infestans recovered from garden huckleberry (solanum scabrum), potato (s. tuberosum) and tomato (lycopersicon esculentum) were inoculated on detached leaves of 12 solanaceous and 14 asteraceous plant species collected in and around potato fields in the western highlands of cameroon. the inoculated leaves were maintained in petri dishes in the laboratory for 7 days at ...200315151295
eggplant latent viroid, the candidate type species for a new genus within the family avsunviroidae (hammerhead viroids).viroids, small circular rnas that replicate independently and in most cases incite diseases in plants, are classified into the families pospiviroidae, composed of species with a central conserved region (ccr) and without hammerhead ribozymes, and avsunviroidae, composed of three members lacking ccr but able to self-cleave in both polarity strands through hammerhead ribozymes. here we report the biological and molecular properties of eggplant latent viroid (elvd). purified circular elvd induces s ...200312743309
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