| effect of pulsed electric field treatment on enzyme kinetics and thermostability of endogenous ascorbic acid oxidase in carrots (daucus carota cv. nantes). | the objective of this research was to study the enzyme kinetics and thermostability of endogenous ascorbic acid oxidase (aao) in carrot purée (daucus carota cv. nantes) after being treated with pulsed electric field (pef) processing. various pef treatments using electric field strength between 0.2 and 1.2kv/cm and pulsed electrical energy between 1 and 520kj/kg were conducted. the enzyme kinetics and the kinetics of aao thermal inactivation (55-70°c) were described using michaelis-menten model a ... | 2014 | 24176379 |
| prediction model for cadmium transfer from soil to carrot (daucus carota l.) and its application to derive soil thresholds for food safety. | at present, soil quality standards used for agriculture do not fully consider the influence of soil properties on cadmium (cd) uptake by crops. this study aimed to develop prediction models for cd transfer from a wide range of chinese soils to carrot (daucus carota l.) using soil properties and the total or available soil cd content. path analysis showed soil ph and organic carbon (oc) content were the two most significant properties exhibiting direct effects on cd uptake factor (ratio of cd con ... | 2013 | 24079518 |
| dissimilarity of contemporary and historical gene flow in a wild carrot (daucus carota) metapopulation under contrasting levels of human disturbance: implications for risk assessment and management of transgene introgression. | transgene introgression from crops into wild relatives may increase the resistance of wild plants to herbicides, insects, etc. the chance of transgene introgression depends not only on the rate of hybridization and the establishment of hybrids in local wild populations, but also on the metapopulation dynamics of the wild relative. the aim of the study was to estimate gene flow in a metapopulation for assessing and managing the risks of transgene introgression. | 2013 | 24052560 |
| cooked carrot volatiles. aeda and odor activity comparisons. identification of linden ether as an important aroma component. | ms with gc-ri evidence was found for the presence of linden ether in cooked carrot (daucus carota). evaluation of the gc effluent from cooked carrot volatiles using aroma extract dilution analysis (aeda) found linden ether with the highest flavor dilution (fd) factor. others with 10-fold lower fd factors were β-ionone, eugenol, the previously unidentified β-damascenone, (e)-2-nonenal, octanal (+ myrcene), and heptanal. all other previously identified volatiles showed lower fd factors. odor thres ... | 2013 | 24000828 |
| theoretical and experimental models of hormetic fusion tubulogenesis. | hormetic morphogens are morphogens such as transforming growth factor beta (tgf-β) in mammals and auxin in plants that induce hormetic responses. for example, in vitro, tgf-β stimulates and inhibits cell proliferation at low and high concentrations respectively. i developed a model of hormetic morphogen gradient control of the morphogenesis of the fusion of bilateral aortic precursors (anlagen) that form the aorta during development; and validated the model with findings obtained by daucus carot ... | 2013 | 23930100 |
| biosynthesis of carotenoids in carrot: an underground story comes to light. | carrot (daucus carota) is a biannual plant that accumulates massive amounts of carotenoid pigments in the storage root. although the root of carrot plants was white before domestication, intensive breeding generated the currently known carotenoid-rich varieties, including the widely popular orange carrots that accumulate very high levels of the pro-vitamin a carotenoids β-carotene and, to a lower extent, α-carotene. recent studies have shown that the developmental program responsible for the acc ... | 2013 | 23876238 |
| dcsto: carrot stowaway-like elements are abundant, diverse, and polymorphic. | we investigated nine families of stowaway-like miniature inverted-repeat transposable elements (mites) in the carrot genome, named dcsto1 to dcsto9. all of them were at-rich and shared a highly conserved 6 bp-long tir typical for stowaways. the copy number of dcsto1 elements was estimated as ca. 5,000 per diploid genome. we observed preference for clustered insertions of dcsto and other mites. distribution of dcsto1 hybridization signals revealed presence of dcsto1 clusters within euchromatic re ... | 2013 | 23775534 |
| quality of carrots as affected by pre- and postharvest factors and processing. | the aim of this review is to provide an update on factors contributing to quality of carrots, with special focus on the role of pre- and postharvest factors and processing. the genetic factor shows the highest impact on quality variables in carrots, causing a 7-11-fold difference between varieties in content of terpenes, β-carotene, magnesium, iron and phenolics as well as a 1-4-fold difference in falcarindiol, bitter taste and sweet taste. climate-related factors may cause a difference of up to ... | 2013 | 23744724 |
| isolation and characterization of a carrot nucleolar protein with structural and sequence similarity to the vertebrate pescadillo protein. | the nuclear matrix is involved in many nuclear events, but its protein architecture in plants is still not fully understood. a cdna clone was isolated by immunoscreening with a monoclonal antibody raised against nuclear matrix proteins of daucus carota l. its deduced amino acid sequence showed about 40% identity with the pescadillo protein of zebrafish and humans. primary structure analysis of the protein revealed a pescadillo n-terminus domain, a single breast cancer c-terminal domain, two nucl ... | 2013 | 23683933 |
| comparison of protective and curative potential of daucus carota root extract on renal ischemia reperfusion injury in rats. | daucus carota linn (apiaceae), a useful vegetable, is traditionally used in treating kidney and hepatic dysfunctions. | 2013 | 23627465 |
| molecular characterisation of a calmodulin gene, vccam1, that is differentially expressed under aluminium stress in highbush blueberry. | calmodulin (cam), a small acidic protein, is one of the best characterised ca(2+) sensors in eukaryotes. this ca(2+) -regulated protein plays a critical role in decoding and transducing environmental stress signals by activating specific targets. many environmental stresses elicit changes in intracellular ca(2+) activity that could initiate adaptive responses under adverse conditions. we report the first molecular cloning and characterisation of a calmodulin gene, vccam1 (vaccinium corymbosum ca ... | 2013 | 23627459 |
| genetic structure and domestication of carrot (daucus carota subsp. sativus) (apiaceae). | | 2013 | 23594914 |
| antibiotic uptake by plants from manure-amended soils. | antibiotics are extensively given to livestock to promote growth and reduce diseases. therefore, animal manure often contains antibiotics. once manure is applied to agricultural land to improve soil productivity, crops would be exposed to antibiotics which may persist in soils from a few to several hundred days. the objective of this study was to evaluate the uptake of gentamicin and streptomycin by carrot (daucus carota), lettuce (lactuca sativa) and radish (rhaphanus sativus) from manure-amend ... | 2013 | 23581689 |
| identification of allergen-resolved threshold doses of carrot (daucus carota) by means of oral challenge and elisa. | | 2013 | 23498592 |
| effect of endogenous ascorbic acid oxidase activity and stability on vitamin c in carrots (daucus carota subsp. sativus) during thermal treatment. | the purpose of this research was to study the effect of endogenous ascorbic acid oxidase (aao) on vitamin c in carrots (daucus carota subsp. sativus), namely nantes, egmont gold and baby carrots during thermal treatment. enzyme-substrate reaction kinetics of aao were described using michaelis-menten equation. the estimated k(m) and v(max) values of aao ranged from 50.34 to 63.54 μm and 23.70 to 26.82 μmol/min, respectively. nantes carrots had the lowest aao activity. on the other hand, egmont go ... | 2012 | 23442658 |
| influence of field attack by carrot psyllid (trioza apicalis förster) on sensory quality, antioxidant capacity and content of terpenes, falcarindiol and 6-methoxymellein of carrots (daucus carota l.). | the effect of different degrees of attack by carrot psyllid (trioza apicalis) on quality parameters of carrots was studied in field experiments for two years. treatments were different degrees of physical insect protection by floating row cover. an increasing attack level of psyllids showed an enhancement effect on the antioxidant capacity (orac), content of falcarindiol, 6-methoxymellein, and terpenes, and scores for bitter taste, chemical flavor, terpene flavor, and toughness. carrot psyllid a ... | 2013 | 23414489 |
| reducing the bioavailability of pcbs in soil to plant by biochars assessed with triolein-embedded cellulose acetate membrane technique. | coupling with triolein-embedded cellulose acetate membrane (tecam) technique, hydroxypropyl β-cyclodextrins (hpcd) extraction method, and the greenhouse pot experiments, the influences of biochars on polychlorinated biphenyls (pcbs) bioavailability in soil to plant (brassica chinensis l. and daucus carota) were investigated. addition of 2% biochars to soils significantly reduced the uptake of pcbs in plant, especially for di-, tri- and tetra-chlorobiphenyls. pcbs concentrations in the roots of b ... | 2013 | 23279904 |
| untargeted metabolomics: an emerging approach to determine the composition of herbal products. | natural remedies, such as those based on traditional chinese medicines, have become more popular also in western countries over the last 10 years. the composition of these herbal products is largely unknown and difficult to determine. moreover, since plants respond to their environment changing the metabolome, the composition of plant material can vary depending on the plant growth conditions. however, there is a growing need of a deeper knowledge on such natural remedies also in view of the gro ... | 2013 | 24688688 |
| [cloning of eleutherococcus senticosus calmodulin gene and effect of endophytic fungus on expression amount of gene]. | to clone calmodulin (cam) gene in eleutherococcus senticosus, and study the effect of endophytic fungi on expression amount of cam gene. | 2012 | 23189731 |
| morphological markers for the detection of introgression from cultivated into wild carrot (daucus carota l.) reveal dominant domestication traits. | hybridisation and subsequent introgression have recently received much attention in the context of genetically modified crops. but crop-wild hybrid detection in the field can be difficult, as most domestication traits seem to be recessive, and the hybrid phenotype may also depend on the direction of the cross or environmental factors. our aim was to develop a reliable set of morphological markers that differ between two wild and 13 cultivated carrots (daucus carota l.) and to evaluate their inhe ... | 2013 | 23173917 |
| clarification and pasteurisation effects on monomeric anthocyanins and percent polymeric colour of black carrot (daucus carota l.) juice. | black carrots (bcs) are a rich source of stable anthocyanins (acns). the purpose of this study was to evaluate the effects of clarification and pasteurisation on acns of black carrot juice (bcj). monomeric acns, acn profile and percent polymeric colour were determined during processing of bcj. while depectinisation and bentonite treatments resulted in 7% and 20% increases in monomeric acn content of bcj, respectively, gelatine-kieselsol treatment and pasteurisation resulted in 10% and 3-16% redu ... | 2012 | 23107727 |
| plants as biofactories: glyphosate-induced production of shikimic acid and phenolic antioxidants in wounded carrot tissue. | the use of plants to produce chemical compounds with pharmaceutical and nutraceutical applications has intensified in recent years. in this regard, genetic engineering is the most commonly used tool to generate crop lines with enhanced concentrations of desirable chemicals. however, growing genetically modified plants is still limited because they are perceived as potential biological hazards that can create an ecological imbalance. the application of postharvest abiotic stresses on plants induc ... | 2012 | 23101679 |
| molecular mapping of vernalization requirement and fertility restoration genes in carrot. | carrot (daucus carota l.) is a cool-season vegetable normally classified as a biennial species, requiring vernalization to induce flowering. nevertheless, some cultivars adapted to warmer climates require less vernalization and can be classified as annual. most modern carrot cultivars are hybrids which rely upon cytoplasmic male-sterility for commercial production. one major gene controlling floral initiation and several genes restoring male fertility have been reported but none have been mapped ... | 2013 | 23015218 |
| a bifasic response to cadmium stress in carrot: early acclimatory mechanisms give way to root collapse further to prolonged metal exposure. | very few studies have provided information about the effects of cadmium (cd) at histoanatomical and ultrastructural levels, along with potential localization of the metal in planta. in particular, from this standpoint, almost nothing is known in daucus carota l. (carrot), a particularly important species for in vitro and in vivo functional investigations. in this work we hypothesized that 36 μm cd, supplied for 1, 2, 3, 4, 7 and 14 days to 30-day-old in vitro-cultured plants, might induce an ear ... | 2012 | 22868210 |
| changes in carrot (daucus carota) parenchyma at higher temperatures detected in vivo by dynamic mechanical (thermal) analysis. | the outer parenchyma of carrot was tested using dynamic mechanical analysis (dma), in air with 90% humidity between 30 and 90°c. temperature plots of storage (sm i.e. elastic) and loss (lm i.e. inelastic) moduli were obtained. the sm and lm values were the basis for the calculation of the loss tangent (lt), the parameter expressing the ratio of inelastic to elastic parenchyma toughness. as expected, the tissue toughness decreased with increasing temperature. for both moduli, two characteristic t ... | 2012 | 22836082 |
| the antioxidant and anticancer effects of wild carrot oil extract. | daucus carota l. ssp. carota (apiacea) is used in traditional medicine in lebanon and in different regions throughout the world. the present study investigates the in vitro anticancer activities of daucus carota oil extract (dcoe) on four human cancer cell lines as well as its in vitro antioxidant activity. dcoe was extracted from the dried umbels with 50:50 acetone-methanol. the oil extract was analyzed by gas chromatography-mass spectrometry and screened for its antioxidant properties in vitro ... | 2013 | 22815230 |
| differential selection on carotenoid biosynthesis genes as a function of gene position in the metabolic pathway: a study on the carrot and dicots. | selection of genes involved in metabolic pathways could target them differently depending on the position of genes in the pathway and on their role in controlling metabolic fluxes. this hypothesis was tested in the carotenoid biosynthesis pathway using population genetics and phylogenetics. | 2012 | 22737218 |
| a protocol to remove colored metabolites and other inhibitors from plant tissues to facilitate rna isolation suitable for downstream applications. | this work developed a protocol to remove colored metabolites and other interfering substances to facilitate rna isolation. these metabolites otherwise hinder rna isolation and downstream applications. the developed protocol used sodium dodecyl sulphate, ethylenediamine tetra-acetic acid, and ethanol in a definite ratio that removed the said metabolites from the tissue and aided isolation of rna using the existing methods. the protocol was developed for red colored roots of arnebia euchroma and w ... | 2012 | 22736512 |
| continuous and long-term monoxenic culture of the arbuscular mycorrhizal fungus gigaspora decipiens in root organ culture. | establishment of arbuscular mycorrhizal (am) germplasm collections is complex because of the obligate biotrophic nature of am fungi. only a few am species are routinely maintained in monoxenic culture with ri t-dna transformed roots as host. incorporation of new am species into this culture system is important for molecular, physiological, and taxonomical studies. here we report for the first time the successful monoxenic culture of gigaspora decipiens (ja2 strain) with transformed carrot (daucu ... | 2012 | 22658317 |
| content of carotenoids in roots of seventeen cultivars of daucus carota l. | the aim of this study was to compare the content of carotenoids in seventeen cultivars of carrots grown in poland. conventional orange cultivars with rarely grown were compared: white, yellow and purple with yellow core cultivars. to determine the content of carotenoids, extracts from lyophilized carrot roots were prepared and analyzed by spectrophotometric as well as hplc methods with dad detector. the highest content of carotenoids was found in cultivars: 'korund f(1)' (48 mg/100g of fresh wei ... | 2012 | 22428130 |
| [obtaining of hairy-root, callus and suspension carrot culture (daucus carota l.) able to accumulate human interferon alpha-2b]. | here we report the obtaining of suspension, callus and hairy root culture initiated from carrot plants of nantskaya and perfektzya variety with the highest level of recombinant human interferon alpha-2b accumulation exhibited the highest level of plant protein extract antiviral activity (up to 12.8 x 10(3) iu/mg tsp). the antiviral activity of callus extracts was significantly lower comparing to the activity of plant extracts from parent organisms. however, the antiviral activity level of suspen ... | 2016 | 22420216 |
| fluorescent in situ hybridization with arbitrarily amplified dna fragments differentiates carrot (daucus carota l.) chromosomes. | carrot (daucus carota l.) chromosomes are small and poorly differentiated in size and morphology. here we demonstrate that fluorescent in situ hybridization (fish) signals derived from arbitrary pcr probes can be used for chromosome identification in carrot. to prepare probes, we searched for nonpolymorphic products abundantly amplified with arbitrary decamer primers in a group of accessions representing carrot genetic diversity. as a result, 13 fragments ranging in size from 517 to 1758 bp were ... | 2012 | 22360760 |
| mitochondrial gene diversity associated with the atp9 stop codon in natural populations of wild carrot (daucus carota ssp. carota). | mitochondrial genomes extracted from the wild populations of daucus carota have been used as a genetic resource by breeders of cultivated carrot, yet little is known concerning the extent of their diversity in nature. of special interest is an snp in the putative stop codon of the mitochondrial gene atp9 that has been associated previously with male-sterile and male-fertile phenotypic variants. in this study, either the sequence or pcr/rflp genotypes were obtained from the mitochondrial genes at ... | 2012 | 22337997 |
| alternative oxidase (aox) and phenolic metabolism in methyl jasmonate-treated hairy root cultures of daucus carota l. | methyl-jasmonate (mj)-treated hairy roots of daucus carota l. were used to study the influence of alternative oxidase (aox) in phenylpropanoid metabolism. phenolic acid accumulation, as well as total flavonoids and lignin content of the mj-treated hairy roots were decreased by treatment with salicylhydroxamic acid (sham), a known inhibitor of aox. the inhibitory effect of sham was concentration dependent. treatment with propyl gallate (pg), another inhibitor of aox, also had a similar inhibitory ... | 2012 | 22326792 |
| effects of genotype, soil type, year and fertilisation on sensory and morphological attributes of carrots (daucus carota l.). | the aim of the study was to investigate the effects of variety, soil type and fertilisation, and the interactions between these factors, on yield and quality traits of carrots. | 2012 | 22231302 |
| polyacetylenes in fresh and stored carrots (daucus carota): relations to root morphology and sugar content. | carrot roots contain polyacetylenes, reported to be both beneficial and distasteful when consumed by humans. this study aimed to investigate the relationships between polyacetylene contents, root morphology and sugar content in order to increase the opportunities to optimise the composition of polyacetylenes in carrots. | 2012 | 22190221 |
| bioactive chemicals from carrot (daucus carota) juice extracts for the treatment of leukemia. | overwhelming evidence indicates that consumption of fruits and vegetables with antioxidant properties correlates with reduced risk for cancers, including leukemia. carrots contain beneficial agents, such as β-carotene and polyacetylenes, which could be effective in the treatment of leukemia. this study investigated the effect of carrot juice extracts on myeloid and lymphoid leukemia cell lines together with normal hematopoietic stem cells. leukemia cell lines and nontumor control cells were trea ... | 2011 | 21864090 |
| spectroscopic studies on bioactive polyacetylenes and other plant components in wild carrot root. | polyacetylenes and other common plant components, such as starch, pectin, cellulose, and lignin, were studied in roots of the wild carrot (daucus carota) subspecies d. carota subsp. gummifer and d. carota subsp. maximus by raman spectroscopy. the components were measured in situ, directly in the plant tissue and without any preliminary sample preparation. the analysis was performed on the basis of the intense and characteristic key bands observed in the raman spectrum. the two main carrot polyac ... | 2011 | 21800857 |
| metabolic fingerprinting reveals differences between shoots of wild and cultivated carrot (daucus carota l.) and suggests maternal inheritance or wild trait dominance in hybrids. | differences between the metabolic content of cultivars and their related wild species not only have implications for breeding and food quality, but also for the increasingly studied area of crop to wild introgression. wild and cultivated western carrots belong to the same outcrossing species and hybridize under natural conditions. the metabolic fingerprinting of dutch wild carrot and of western orange carrot cultivar shoots using (1)h nmr showed only quantitative differences in chemical content, ... | 2011 | 21601898 |
| plants as biofactories: physiological role of reactive oxygen species on the accumulation of phenolic antioxidants in carrot tissue under wounding and hyperoxia stress. | plants subjected to postharvest abiotic stresses synthesize secondary metabolites with health-promoting properties. here, we report the potential use of carrots (daucus carota) as biofactories of caffeoylquinic acids when subjected to wounding and hyperoxia stresses. wounding stress induced an increase of ∼287% in total phenolic content (pc) in carrots stored for 48 h at 20 °c. this increase was higher (∼349%) in the wounded tissue treated with hyperoxia stress. to further understand the physiol ... | 2011 | 21553806 |
| comparative fish mapping of daucus species (apiaceae family). | the cytogenetic characterization of the carrot genome (daucus carota l., 2n = 18) has been limited so far, partly because of its somatic chromosome morphology and scant of chromosome markers. here, we integrate the carrot linkage groups with pachytene chromosomes by fluorescent in situ hybridization (fish) mapping genetically anchored bacterial artificial chromosomes (bacs). we isolated a satellite repeat from the centromeric regions of the carrot chromosomes, which facilitated the study of the ... | 2011 | 21547583 |
| [qtl mapping for contents of main carotenes and lycopene in carrot (daucus carota l.)]. | an f2 population derived from two carrot inbred lines, p50006 and hcm a.c. with high carotene accumulation, was developed and used to map and analyze quantitative trait locus (qtl) associated with the accumulation of alpha and beta-carotene, total carotene and lycopene. broad-sense heritabilities of these traits were 0.75, 0.50, 0.31, and 0.93, respectively. a genetic map with 91 srap (sequence-related amplified polymorphism) markers was developed, which spanned 502.9 cm in 9 linkage groups with ... | 2010 | 21513156 |
| effect of processing parameters on physico-chemical and culinary quality of dried carrot slices. | the investigation was carried out to evaluate the carrot (daucus carota) cultivars, to optimize the pre-treatments, time and temperature combination for drying carrot slices and to assess the suitability of the dried product for culinary preparations. among the 4 cultivars, ('pc-34', 'sel-21', 'ambala local' and 'nantes') the last one showed the best physico-chemical characteristics for dehydration. the dried carrot slices with highly desirable physico-chemical characteristics could be prepared ... | 2011 | 23572730 |
| differences in protodermal cell wall structure in zygotic and somatic embryos of daucus carota (l.) cultured on solid and in liquid media. | the ultrastructure, cuticle, and distribution of pectic epitopes in outer periclinal walls of protodermal cells of daucus carota zygotic and somatic embryos from solid and suspension culture were investigated. lipid substances were present as a continuous layer in zygotic and somatic embryos cultured on solid medium. somatic embryos from suspension cultures were devoid of cuticle. the ultrastructure of the outer walls of protodermis of embryos was similar in zygotic and somatic embryos from soli ... | 2012 | 21424614 |
| anthocyanin composition of black carrot (daucus carota ssp. sativus var. atrorubens alef.) cultivars antonina, beta sweet, deep purple, and purple haze. | this study aimed to identify the pigment composition of black carrot (daucus carota ssp. sativus var. atrorubens alef.) cultivars antonina, beta sweet, deep purple, and purple haze. cyanidin 3-xylosyl(glucosyl)galactosides acylated with sinapic acid, ferulic acid, and coumaric acid were detected as major anthocyanins by high-performance liquid chromatography with diode array detection (hplc-dad) and with electrospray ionization multiple mass spectrometry (hplc-esi-ms(n)) analyses. the preparativ ... | 2011 | 21381748 |
| [influence of arbuscular mycorrhizal inoculation on growth and phoxim residue of carrot (daucus carota l.)]. | a pot culture experiment was carried out to study the influence of arbuscular mycorrhizal (am) fungi on the growth and phoxim residue of carrot (daucus carota l). four levels of phoxim (0, 200, 400, 800 mg x l(-1)) and two am fungal inocula, glomus intraradices beg 141(141), glomus mosseae beg 167 (167),and one nonmycorrhizal inoculum (ck), were applied to the sterilized soil. the plants were harvested after 5 months of growth and phoxim was irrigated into the root zone 14 d before plant harvest ... | 2010 | 21360902 |
| effects of ten antibiotics on seed germination and root elongation in three plant species. | we applied a screening-level phytotoxicity assay to evaluate the effects of 10 antibiotics (at concentrations ranging from 1 to 10,000 μg/l) on germination and early plant growth using three plant species: lettuce (lactuca sativa), alfalfa (medicago sativa), and carrot (daucus carota). the range of phytotoxicity of the antibiotics was large, with ec₂₅s ranging from 3.9 μg/l to >10,000 μg/l. chlortetracycline, levofloxacin, and sulfamethoxazole were the most phytotoxic antibiotics. d. carota was ... | 2011 | 21107831 |
| comparison of two liquid-state nmr methods for the determination of saccharides in carrot (daucus carota l.) roots. | in order to determine the saccharide content of plant tissues, we studied a new non-destructive and fast analytical method that we call "direct quantitative proton nuclear magnetic resonance spectroscopy" (d q (1)h nmr): the application of quantitative proton nuclear magnetic resonance spectroscopy (q (1)h nmr) to non modified plant tissues along with capillary tubes containing a reference compound (for quantification) and deuterium oxide (for locking). using this method, the saccharide content ... | 2011 | 21046086 |
| effects of harvesting date and storage on the amounts of polyacetylenes in carrots, daucus carota. | the amounts of three main polyacetylenes in carrots; falcarinol, falcarindiol, and falcarindiol-3-acetate, were determined by hplc, during three seasons, in carrots harvested several times per season and at different locations in sweden. the amounts of falcarindiol first decreased from a relatively high level and then increased later in the harvest season. the amounts of falcarindiol-3-acetate showed similar variations, whereas the amounts of falcarinol did not exhibit any significant variation ... | 2010 | 20964297 |
| arbuscular mycorrhizal hyphopodia and germinated spore exudates trigger ca2+ spiking in the legume and nonlegume root epidermis. | • the aim of this study was to investigate ca(2+) responses to endosymbiotic arbuscular mycorrhizal (am) fungi in the host root epidermis following pre-infection hyphopodium formation in both legumes and nonlegumes, and to determine to what extent these responses could be mimicked by germinated fungal spore exudate. • root organ cultures of both medicago truncatula and daucus carota, expressing the nuclear-localized cameleon reporter nupyc2.1, were used to monitor am-elicited ca(2+) responses in ... | 2011 | 20880223 |
| influence of boron on carrot cell wall structure and its resistance to fracture. | plant cell wall structure integrity and associated tissue mechanical properties is one of key determinants for the perceived texture of plant-based foods. carrots (daucus carota) were used to investigate the effect of mineral supply of boron (b) and/or calcium (ca), during plant growth, on the plant cell wall structure and mechanical properties of matured root tissues. five commercial cultivars of carrots, kuroda (orange), dragon purple, kuttiger white, yellow, and nutri-red, were cultivated und ... | 2010 | 23654242 |
| effect of carrot (daucus carota) microstructure on carotene bioaccessibilty in the upper gastrointestinal tract. 1. in vitro simulations of carrot digestion. | studies investigating carotene bioaccessibility (release from the food matrix to a solubilized form) directly from plant material during the process of digestion are scarce, mainly due to the difficulties associated with obtaining such material. therefore, this paper examines the relationship between tissue microstructure and carotene bioaccessibility using an in vitro digestion model. dietary oil provides a pool for the initial solubilization. therefore, carotene partitioning into an emulsified ... | 2010 | 20698537 |
| historical and contemporary gene dispersal in wild carrot (daucus carota ssp. carota) populations. | wild carrot is the ancestor of cultivated carrot and is the most important gene pool for carrot breeding. transgenic carrot may be released into the environment in the future. the aim of the present study was to determine how far a gene can disperse in wild carrot populations, facilitating risk assessment and management of transgene introgression from cultivated to wild carrots and helping to design sampling strategies for germplasm collections. | 2010 | 20566679 |
| nutritional, physical, and sensory evaluation of hydroponic carrots (daucus carota l.) from different nutrient delivery systems. | carrot (daucus carota l.) has the highest carotenoid content among foods and is consumed in large quantities worldwide, while at the same time its market demand continues to increase. carotenoids have also been associated with protective effects against cancer and other chronic diseases. the most predominant carotenoids in carrots are beta- and alpha-carotenes. moisture, ash, fat, texture, color, carotene content, and consumer acceptance of carrots grown in a hydroponic system with nutrient film ... | 2013 | 20492130 |
| obtaining carrot (daucus carota l.) plants in isolated microspore cultures. | microspores were cultured on the modified b5 liquid medium containing 2.4d (0.1 mg l(-1)), naa (0.1 mg l(-1)), l-glutamine (500 mg l(-1), l-serine (100 mg l(-1)), and sucrose (100 g l(-1)). the developmental stages of microspores and divisions were observed. initially, the formation of binuclear and multicellular structures was noticed. plants regenerated in the cultures in which the tetrad stage of microsporogenesis had predominated. embryoids were still forming 24 weeks after the cultures were ... | 2010 | 20453301 |
| carotenoid biosynthesis genes provide evidence of geographical subdivision and extensive linkage disequilibrium in the carrot. | according to the history of the cultivated carrot, root colour can be considered as a structural factor of carrot germplasm. therefore, molecular variations of carotenoid biosynthesis genes, these being involved in colour traits, represent a good putative source of polymorphism related to diversity structure. seven candidate genes involved in the carotenoid biosynthesis pathway have been analysed from a sample of 48 individual plants, each one from a different cultivar of carrot (daucus carota l ... | 2010 | 20411232 |
| effectiveness of different edu concentrations in ameliorating ozone stress in carrot plants. | ethylenediurea (edu) is suggested for use to evaluate plant response under ambient ozone (o(3)) concentrations. four edu treatments, viz. 0 (non-edu), 150, 300 and 450 mg l(-1), applied as soil drench at 10 days interval to carrot (daucus carota l. var. pusa kesar), grown at a tropical suburban site of varanasi experiencing mean o(3) concentration of 36.1 ppb during the experimental period. edu treated plants showed significantly higher antioxidative defense, assimilation capability and reduced ... | 2010 | 20403638 |
| effect of storage and cooking on beta-carotene isomers in carrots ( daucus carota l. cv. 'stefano'). | carrots are one of the highest dietary sources of beta-carotene and are naturally high in the (all-e)-beta-carotene isomer, which has higher bioavailability, provitamin a activity, and antioxidant capacity compared to z (cis) isomers. the objectives of the present study were to investigate the effects of storage temperature, time, and cooking (boiling for 15 min) on the levels of carotene isomers in 'stefano' carrots. storing carrots at either 4 degrees c to simulate long-term storage or 20 degr ... | 2010 | 20359248 |
| anthocyanins in purple-orange carrots (daucus carota l.) do not influence the bioavailability of beta-carotene in young women. | purple carrots contain anthocyanins in addition to the provitamin a carotenoids in typical orange carrots. simultaneous consumption of these phytochemicals in carrots may affect the bioavailability of carotenoids. the bioavailability of beta-carotene in humans was assessed from an acute feeding of orange (oc) and purple (pc) carrots with white (wc) as a control. carrot smoothies were served to female subjects (n = 5, aged 21-26 years) for breakfast after 1 week on a low carotenoid diet and overn ... | 2010 | 20131807 |
| specific developmental pathways underlie host specificity in the parasitic plant orobanche. | parasitic angiosperms are an ecologically and economically important group of plants. however our understanding of the basis for host specificity in these plants is embryonic. recently we investigated host specificity in the parasitic angiosperm orobanche minor, and demonstrated that this host generalist parasite comprises genetically defined races that are physiologically adapted to specific hosts. populations occurring naturally on red clover (trifolium pratense) and sea carrot (daucus carota ... | 2010 | 20081361 |
| two new guaiane-type sesquiterpene glycosides from the fruits of daucus carota l. | two new guaiane-type sesquiterpene glycosides, 11-o-acetyl-torilolone 8-o-beta-d-glucopyranoside (1) and 1beta-hydroxytorilolone 11-o-beta-d-glucopyranoside (2), were isolated from the fruits of daucus carota l. their chemical structures were elucidated on the basis of ms, nmr spectroscopic analyses coupled with chemical degradation. | 2010 | 20045981 |
| polyacetylene diversity and bioactivity in orange market and locally grown colored carrots (daucus carota l.). | carrots contain a wide array of phytochemicals such as carotenoids, phenolics, alpha-tocopherol, and polyacetylenes. carrots are most known for their pro-vitamin a carotenoids but also contain other phytochemicals with documented health benefits. the phytochemicals in colored carrots present a challenge and opportunity due to the wide diversity of potent bioactive compounds. two commercial carrots, 1 wild carrot, and 13 colored carrot varieties were characterized phytochemically. the carrots wer ... | 2009 | 19950995 |
| carrot alternative oxidase gene aox2a demonstrates allelic and genotypic polymorphisms in intron 3. | single nucleotide polymorphisms (snps) and insertion-deletions (indels) are becoming important genetic markers for major crop species. in this study, we focus on variations at genomic level of the daucus carota l. aox2a gene. the use of gene-specific primers designed in exon regions on the boundaries of introns permitted to recognize intron length polymorphism (ilp) in intron 3 aox2a by simple polymerase chain reaction (pcr) assays. the length of intron 3 can vary in individual carrot plants. th ... | 2009 | 19941625 |
| quantitative permeability imaging of plant tissues. | a method for mapping tissue permeability based on time-dependent diffusion measurements is presented. a pulsed field gradient sequence to measure the diffusion encoding time dependence of the diffusion coefficients based on the detection of stimulated spin echoes to enable long diffusion times is combined with a turbo spin echo sequence for fast nmr imaging (mri). a fitting function is suggested to describe the time dependence of the apparent diffusion constant in porous (bio-)materials, even if ... | 2010 | 19921172 |
| response to uv-c radiation in topo i-deficient carrot cells with low ascorbate levels. | in animal cells, recent studies have emphasized the role played by dna topoisomerase i (topo i) both as a cofactor of dna repair complexes and/or as a damage sensor. all these functions are still unexplored in plant cells, where information concerning the relationships between dna damage, pcd induction, and topo i are also limited. the main goal of this study was to investigate the possible responses activated in topo i-depleted plant cells under oxidative stress conditions which induce dna dama ... | 2010 | 19917599 |
| alternative oxidase involvement in daucus carota somatic embryogenesis. | plant alternative oxidase (aox) is a mitochondrial inner membrane enzyme involved in alternative respiration. the critical importance of the enzyme during acclimation upon stress of plant cells is not fully understood and is still an issue of intensive research and discussion. recently, a role of aox was suggested for the ability of plant cells to change easily its fate upon stress. in order to get new insights about aox involvement in cell reprogramming, quantitative real-time polymerase chain ... | 2009 | 19863756 |
| structure determination of bisacetylenic oxylipins in carrots (daucus carota l.) and enantioselective synthesis of falcarindiol. | although bisacetylenic oxylipins have been demonstrated to exhibit diverse biological activities, the chemical structures of many representatives of this class of phytochemicals still remain elusive. as carrots play an important role in our daily diet and are known as a source of bisacetylenes, an extract made from daucus carota l. was screened for bisacetylenic oxylipins, and, after isolation, their structures were determined by means of lc-ms and 1d/2d nmr spectroscopy. besides the previously ... | 2009 | 19845355 |
| gene transcription analysis of carrot allergens by relative quantification with single and duplex reverse transcription real-time pcr. | single and duplex real-time polymerase chain reaction (pcr) systems have been developed to quantify specific mrna transcription of genes coding for the major daucus carota allergen isoforms dau c 1.01 and dau c 1.02. methods were tested with samples from the local market. whereas the gene transcription levels for dau c 1.01 were consistently high in all investigated samples, significant differences for the dau c 1.02 transcription could be demonstrated in randomly collected market samples. the g ... | 2010 | 19834694 |
| differential expression and co-regulation of carrot aox genes (daucus carota). | alternative oxidase (aox) is a mitochondrial protein encoded by the nuclear genome. in higher plants aox genes form a small multigene family mostly consisting of the two subfamilies aox1 and aox2. daucus carota l. is characterized by a unique extension pattern of aox genes. different from other plant species studied so far it contains two genes in both subfamilies. therefore, carrot was recently highlighted as an important model in aox stress research to understand the evolutionary importance of ... | 2009 | 19825008 |
| splinkbes: a splinkerette-based method for generating long end sequences from large insert dna libraries. | we report on the development of a novel splinkerette-based method for generating long end sequences from large insert library clones, using a carrot (daucus carota l.) bac library as a model. the procedure involves digestion of the bac dna with a 6-bp restriction enzyme, followed by ligation of splinkerette adaptors that serve as primer-annealing sites for pcr amplification of the bac ends. the resulting amplicons are sequenced from both directions, and when overlapping, the pairs of sequences a ... | 2009 | 19737131 |
| a new sesquiterpene from the fruits of daucus carota l. | phytochemical investigation of the fruits of daucus carota l. resulted in the isolation of a new sesquiterpene named as daucucarotol (1). its structure was elucidated on the basis of 1d and 2d nmr experiments, coupled with ms studies. to our knowledge, compound 1 is the first example for a natural eudesmane sesquiterpene with a hydroxymethyl group located at a methine carbon rather than a usual quaternary carbon in the two fused six-membered ring systems. | 2009 | 19701130 |
| development and evaluation of monoclonal antibodies as probes to assess the differences between two tomato pectin methylesterase isoenzymes. | the enzyme pectin methylesterase (pme) was purified from red ripe tomatoes (lycopersicon esculentum) and through affinity chromatography two isoenzymes were fractionated (t1pme and t2pme). further analysis of these two isoenzymes, both having a molar mass of 34.5kda, revealed a difference in the n-terminal sequence and in amino acid composition. t1pme was identified as the major isoenzyme of pme in tomato fruit. in this study the aim was to develop a toolbox, consisting of monoclonal antibodies, ... | 2009 | 19686752 |
| suboptimal temperature favors reserve formation in biennial carrot (daucus carota) plants. | in response to suboptimal temperatures, temperate annual plants often increase root:shoot ratios, build-up carbohydrates and display typical morphological and anatomical changes. we know less about the responses of biennials such as carrot. as a model plant, carrot has the additional feature of two functionally and morphologically distinct root parts: the taproot, which stores carbohydrate and other compounds, and the fibrous root system involved in acquisition of water and nutrients. here, we a ... | 2009 | 19493310 |
| host-driven divergence in the parasitic plant orobanche minor sm. (orobanchaceae). | many parasitic angiosperms have a broad host range and are therefore considered to be host generalists. orobanche minor is a nonphotosynthetic root parasite that attacks a range of hosts from taxonomically disparate families. in the present study, we show that o. minor sensu lato may comprise distinct, genetically divergent races isolated by the different ecologies of their hosts. using a three-pronged approach, we tested the hypothesis that intraspecific taxa o. minor var. minor and o. minor ss ... | 2008 | 19378406 |
| antioxidant phytochemicals and antioxidant capacity of biofortified carrots (daucus carota l.) of various colors. | antioxidants and antioxidant capacity of seven colored carrots were determined. five anthocyanins, chlorogenic acid, caffeic acid, and four carotenoids were quantified by hplc. total phenolic content was determined according to the folin-ciocalteu method. antioxidant capacities of the hydrophilic and hydrophobic fractions were determined by using the 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (abts) and 2,2'-diphenyl-1-picrylhydrazyl (dpph) methods. the relative antioxidant capacity i ... | 2009 | 19358535 |
| accumulation of p-hydroxybenzoic acid in hairy roots of daucus carota 2: confirming biosynthetic steps through feeding of inhibitors and precursors. | biosynthesis of hydroxybenzoates even at enzymatic level is poorly understood. in this report, effect of feeding of putative biosynthetic precursors and pathway-specific enzyme inhibitors of early phenylpropanoid pathway on p-hydroxybenzoic acid accumulation in chitosan-elicited hairy roots of daucus carota was studied. three selective metabolic inhibitors of plant phenylpropanoid pathway, namely, aminooxyacetic acid (aoaa), piperonylic acid (pip) and 3,4-methylenedioxycinnamic acid (mdca), whic ... | 2009 | 19342120 |
| metabolomics of daucus carota cultured cell lines under stressing conditions reveals interactions between phenolic compounds. | a metabolomic approach followed by principal components and partial least square analysis was used for investigating the effect of environmental factors on two daucus carota l. cv. flakkese cell lines (r3m and r4g), selected for their ability to produce anthocyanins in the light and the dark, respectively. a positive correlation between total anthocyanin, hydroxycinnamic and hydroxybenzoic acid accumulation was found in both lines. furthermore, the experimental design and the combination of bioc ... | 2009 | 26493146 |
| possible involvement of dna methylation on expression regulation of carrot lec1 gene in its 5'-upstream region. | dna methylation plays important roles in various developmental processes in many organisms. in carrots, the treatment of embryogenic cells (ecs) with dna methylation inhibitors induces hypomethylation and blocks somatic embryogenesis. carrot-leafy cotyledon 1 (c-lec1) is an important transcription factor for embryo development that shows embryo-specific expression in ecs and somatic and zygotic embryos. however, the regulation of embryo-specific transcription factor genes such as c-lec1 in plant ... | 2009 | 19264116 |
| host-specific races in the holoparasitic angiosperm orobanche minor: implications for speciation in parasitic plants. | orobanche minor is a root-holoparasitic angiosperm that attacks a wide range of host species, including a number of commonly cultivated crops. the extent to which genetic divergence among natural populations of o. minor is influenced by host specificity has not been determined previously. here, the host specificity of natural populations of o. minor is quantified for the first time, and evidence that this species may comprise distinct physiological races is provided. | 2009 | 19251714 |
| daucus carota mediated-reduction of cyclic 3-oxo-amines. | carrots (daucus carota) were used to reduce cyclic amino-ketones in high yields and enantiomeric excesses. this cheap, eco-compatible, and efficient reducing reagent allows the easy access to precursors of biologically active products. | 2009 | 19215083 |
| molecular characterization of two alphacryptovirus dsrnas isolated from daucus carota. | | 2009 | 19165417 |
| a study on the effect of few eco-friendly manures on the growth attributes of carrot (daucus carota l.). | a pot culture study was carried out at avinashilingam deemed university, coimbatore to assess the effect of few organic manures (herbal microfertilizer, humic acid, farm yard manure (fym), biofertilizer) and npk on the growth attributes of carrot. among the parameters observed, the germination percentage was highest in fym treated pots on 7 das (days after sowing) and in npk treated pots on 21, 28 and 30 das. the parameters, such as root length, crown length and vigour index, were studied on 30, ... | 2009 | 21114148 |
| fungal plant pathogens in portugal: alternaria dauci. | a leaf blight of carrot (daucus carota) caused by alternaria dauci was found in alentejo (ourique, portugal). morphological characteristics of the fungus are described. | 2008 | 19071897 |
| sulfur transfer through an arbuscular mycorrhiza. | despite the importance of sulfur (s) for plant nutrition, the role of the arbuscular mycorrhizal (am) symbiosis in s uptake has received little attention. to address this issue, 35s-labeling experiments were performed on mycorrhizas of transformed carrot (daucus carota) roots and glomus intraradices grown monoxenically on bicompartmental petri dishes. the uptake and transfer of 35so4(2-) by the fungus and resulting 35s partitioning into different metabolic pools in the host roots was analyzed wh ... | 2009 | 18978070 |
| reinvestigation of the bitter compounds in carrots (daucus carota l.) by using a molecular sensory science approach. | in order to reinvestigate the key molecules inducing bitter off-taste of carrots ( daucus carota l.), a sensory-guided fractionation approach was applied to bitter carrot extracts. besides the previously reported bitter compounds, 6-methoxymellein (1), falcarindiol (2), falcarinol (3), and falcarindiol-3-acetate (4), the following compounds were identified for the first time as bitter compounds in carrots with low bitter recognition thresholds between 8 and 47 micromol/l: vaginatin (5), isovagin ... | 2008 | 18922011 |
| isolation of development-related genes in somatic embryo radicle of carrot (daucus carota l.) using modified cdna rda. | using modified cdna rda capitalizing on the high affinity of streptavidin for biotin and magnetic-absorption-based separation, we have obtained four bands of specifically expressed cdna in the carrot somatic embryo deregulated for 12 h, which were designated as nr-1, nr-2, nr-3 and nr-4, respectively. as revealed by homology analysis of their dna sequences after cloning them into pbs, remarkable homology was demonstrated in nr-2, nr-3 and nr-4 with the genes coding for lea (late embryogenesis ab ... | 2000 | 18763115 |
| effect of decontamination agents on the microbial population, sensorial quality, and nutrient content of grated carrots (daucus carota l.). | several decontamination agents including water, sodium hypochlorite, peroxyacetic acid, neutral electrolyzed oxidizing water, and chlorine dioxide gas were tested for their effectiveness to reduce the natural microflora on grated carrots. microbial reductions of the total aerobic count obtained after the different treatments varied between 0.11 and 3.29 log colony-forming units (cfu)/g. whether or not a decontamination step induced significant changes in the sensory attributes of grated carrots ... | 2008 | 18582083 |
| diversity of dcmaster-like elements of the pif/harbinger superfamily in the carrot genome. | transposable elements constitute a significant fraction of plant genomes. both autonomous and non-autonomous elements of the dcmaster family, residing in the carrot genome, were described previously. dcmaster elements were classified as members of the pif/harbinger superfamily. in the present paper we report on the identification of other groups of dcmaster-like elements. beside the previously described, relatively highly abundant miniature inverted repeat element (mite) family krak (ca. 3,600 c ... | 2009 | 18535910 |
| color evolution of aqueous solutions obtained by thermal processing of carrot (daucus carota l.) roots: influence of light. | the color of aqueous solutions obtained by heating carrot (daucus carota l.) roots in water ("stocks") is different when the thermal treatment is applied with or without exposure to light. cie l*, a*, and b* scale values of stocks processed for different times were recorded and 4 patterns were initially observed. to explain the 1st part of this evolution (patterns 1 and 2), pectin extraction and beta-elimination in stocks were studied. light dependence was investigated to explain patterns 3 and ... | 2008 | 18460127 |
| spatial and temporal expression profiling of cell-wall invertase genes during early development in hybrid poplar. | cell-wall invertase genes are spatially and temporally regulated in several plant species, including daucus carota l., lycopersicon esculentum l. and solanum tuberosum l. however, few studies of cell-wall invertase genes of trees have been conducted, despite the importance of trees as a source of lignocellulosic biopolymers. we identified three putative cell-wall invertase genes in hybrid poplar (populus alba l. x grandidentata michx.) that showed higher homology to each other than to cell-wall ... | 2008 | 18450570 |
| a carrot g-box binding factor-type basic region/leucine zipper factor dcbz1 is involved in abscisic acid signal transduction in somatic embryogenesis. | carrot (daucus carota) somatic embryogenesis has been extensively used as an experimental system for studying embryogenesis. in maturing zygotic embryos, abscisic acid (aba) is involved in acquisition of desiccation tolerance and dormancy. on the other hand, somatic embryos contain low levels of endogenous aba and show desiccation intolerance and lack dormancy, but tolerance and dormancy can be induced by exogenous application of aba. in aba-treated carrot embryos, some aba-inducible genes are e ... | 2016 | 18407508 |
| survival and flowering of hybrids between cultivated and wild carrots (daucus carota) in danish grasslands. | many crop species are able to hybridize with related weedy or wild relatives, which could lead to transfer of cultivar genes, and among them transgenes, into wild populations. it is not clear, however, whether the hybrids and their descendants are able to survive and reproduce in natural habitats, as inherited cultivar traits may be maladaptive under such conditions. to test this, we produced hybrid (f(1)) seeds by controlled crosses between wild [see text for formula] and cultivated carrots (da ... | 2008 | 18289499 |
| dcmaster transposon display markers as a tool for diversity evaluation of carrot breeding materials and for hybrid seed purity testing. | in recent years, transposon insertion polymorphisms have been utilized as molecular markers, and a range of techniques tailored towards identification of insertion sites of various transposable elements have been developed. in the present paper we describe the application of a recently developed dcmaster transposon display system to analyse the genetic diversity of polish breeding materials of carrot (daucus carota) and to identify polymorphisms useful for hybrid seed purity testing. using 3 set ... | 2008 | 18263967 |
| determination of kinetic and equilibrium parameters of the batch adsorption of mn(ii), co(ii), ni(ii) and cu(ii) from aqueous solution by black carrot (daucus carota l.) residues. | in this study, the effect of temperature on the adsorption of mn(ii), ni(ii), co(ii) and cu(ii) from aqueous solution by modified carrot residues (mcr) was investigated. the equilibrium contact times of adsorption process for each heavy metals-mcr systems were determined. kinetic data obtained for each heavy metal by mcr at different temperatures were applied to the lagergren equation, and adsorption rate constants (kads) at these temperatures were determined. these rate constants related to the ... | 2008 | 17980960 |
| improvement of texture properties and flavor of frozen dough by carrot (daucus carota) antifreeze protein supplementation. | the effects of concentrated carrot protein (ccp) containing 15.4% (w/w) carrot (daucus carota) antifreeze protein on texture properties of frozen dough and volatile compounds of crumb were studied. ccp supplementation lowered the freezable water content of the dough, resulting in some beneficial effects including holding loaf volume steadily and making the dough softer and steadier during frozen storage. furthermore, spme-gc-ms analysis showed ccp supplementation did not give any negative influe ... | 2007 | 17935294 |
| simultaneous modelling of the thermal degradation kinetics of pectin methylesterase in lettuce (lactuca sativa l.) and carrot (daucus carota l.) extracts: analysis of seasonal variation and tissue type. | the thermal degradation kinetics of pectin methylesterase (pme) from carrot and lettuce were studied. fresh extracts were exposed to temperatures from 55 to 70 degrees c until the enzyme was inactivated. a model based on the presence of two forms of the enzyme, one active and one non-active, is proposed. the natural variability of the pme activity was taken into the model in the form of normally distributed random effects. the common model parameters obtained (cleavage constant (0.0395+/-0.0062 ... | 2007 | 17928712 |
| cloning and expression of dcga gene encoding alpha subunit of gtp-binding protein in carrot seedlings. | a homology-based cloning strategy yielded a cdna clone designated dcga, presumably encoding alpha subunit of gtp-binding protein, from carrot (daucus carota) seedlings. molecular phylogenetic tree analysis of g protein alpha subunits from various biological sources suggested that, unlike in animal cells, classification of galpha proteins into specific subfamilies could not be applicable to the proteins from higher plants. the restriction digests prepared from genomic dna of carrot showed one or ... | 2007 | 17827744 |
| evidence for p-hydroxybenzoate formation involving enzymatic phenylpropanoid side-chain cleavage in hairy roots of daucus carota. | in this study, methyl jasmonate (mj)-elicited hairy root cultures of daucus carota were explored to study the enzymatic route to p-hydroxybenzoic acid (p-hba) biosynthesis. treatment with 100microm mj caused an enhanced accumulation of p-hba as well as total phenolic content in elicited root lines as compared to untreated (controls) lines. using cell-free extract as the source of crude enzymes, attempt was made to reveal the enzymatic route to p-hba formation. the accumulation of p-hba was prece ... | 2008 | 17658659 |
| differential effects of hexaconazole and paclobutrazol on biomass, electrolyte leakage, lipid peroxidation and antioxidant potential of daucus carota l. | the application of triazole fungicides is a common practice in the cultivation of carrot (daucus carota l.) plants. it is there for seems important to test the changes that are occurring in this food crop under triazoles, the non-traditional plant growth regulators, treatments in order to identify the extent to which it tolerate the fungicide application and thereby make it an economical food crop. a field experiment was conducted to find out the effects of two triazole fungicides (hexaconazole ... | 2007 | 17644352 |
| determination of polyacetylenes in carrot roots (daucus carota l.) by high-performance liquid chromatography coupled with diode array detection. | a new high-performance liquid chromatographic method with diode array detection was developed for the separation and simultaneous determination of the carrot polyacetylenes falcarindiol (fadoh), falcarindiol 3-acetate (fadoac) and falcarinol (faoh) in carrot root extracts. the optimal chromatographic conditions were achieved on a c18 column with a linear gradient elution of water and acetonitrile as mobile phases, at the flow rate of 1.0 ml/min. all calibration curves of the three carrot polyace ... | 2007 | 17444217 |