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elevated carbon dioxide altered morphological and anatomical characteristics, ascorbic acid accumulation, and related gene expression during taproot development in carrots.the co2 concentration in the atmosphere has increased significantly in recent decades and is projected to rise in the future. the effects of elevated co2 concentrations on morphological and anatomical characteristics, and nutrient accumulation have been determined in several plant species. carrot is an important vegetable and the effects of elevated co2 on carrots remain unclear. to investigate the effects of elevated co2 on the growth of carrots, two carrot cultivars ('kurodagosun' and 'deep pu ...201628119712
wild carrot pentane-based fractions suppress proliferation of human hacat keratinocytes and protect against chemically-induced skin cancer.previous studies in our laboratory showed that the lebanese daucus carota ssp. carota (wild carrot) oil extract possesses in vitro and in vivo anticancer activities. the present study aims to examine the cytotoxic effect of daucus carota oil fractions on human epidermal keratinocytes and evaluate the chemopreventive activity of the pentane diethyl ether fraction on dmba/tpa induced skin carcinogenesis in mice.201728073348
thermal time model for egyptian broomrape (phelipanche aegyptiaca) parasitism dynamics in carrot (daucus carota l.): field validation.carrot, a highly profitable crop in israel, is severely damaged by phelipanche aegyptiaca parasitism. herbicides can effectively control the parasite and prevent damage, but for optimal results, knowledge about the soil-subsurface phenological stage of the parasite is essential. parasitism dynamics models have been successfully developed for the parasites p. aegyptiaca, orobanche cumana, and orobanche minor in the summer crops, tomato, sunflower, and red clover, respectively. however, these mode ...201628018371
carotenoid gene expression explains the difference of carotenoid accumulation in carrot root tissues.main conclusion variations in gene expression can partially explain the difference of carotenoid accumulation in secondary phloem and xylem of fleshy carrot roots. the carrot root is well divided into two different tissues separated by vascular cambium: the secondary phloem and xylem. the equilibrium between these two tissues represents an important issue for carrot quality, but the knowledge about the respective carotenoid accumulation is sparse. the aim of this work was (i) to investigate if v ...201727999990
ionizing radiation from chernobyl affects development of wild carrot plants.radioactivity released from disasters like chernobyl and fukushima is a global hazard and a threat to exposed biota. to minimize the deleterious effects of stressors organisms adopt various strategies. plants, for example, may delay germination or stay dormant during stressful periods. however, an intense stress may halt germination or heavily affect various developmental stages and select for life history changes. here, we test for the consequence of exposure to ionizing radiation on plant deve ...201627982121
assessment and accessibility of phenotypic and genotypic diversity of carrot (daucus carota l. var. sativus) cultivars commercially available in the united states.increased use of intellectual property rights over plant germplasm has led to a complicated landscape for exchange among plant breeders. our goal was to examine phenotypic and genotypic diversity present in commercially available carrot (daucus carota l. var. sativus) germplasm in relation to the freedom to operate-the ability for plant breeders to access and use crop genetic diversity. a collection of 140 commercially available carrot cultivars were grown in replicated field trials in the madis ...201627959910
glutathione sulfotransferase inhibition activity of a self-fermented beverage, kanji.kanji, a liquid preparation of roots of daucus carota l. ssp. sativus (hoffm.) arcang. var. vavilovii mazk. (apiaceae), may inhibit glutathione sulfotransferase (gst) activity due to ferulic acid content.201727951746
first report of leafminer lyriomyza spp. in daucus carota in brazil. 201627849253
bioassessment of heavy metals in the surface soil layer of an opencast mine aimed for its rehabilitation.the contemporary reclamation method in an opencast coal mine closure comprises the use of the preserved surface soil layer (ssl) before mining, and can be directly returned to the areas being rehabilitated. the present study emphasizes a risk in the use of such a ssl in mine rehabilitation due to the possible excessive amount of heavy metals which usually derives from a metal-rich sediment or fluvial character of overburden material. this indication was approved by the bioassessment of cadmium ( ...201727823905
carrot solution culture bioproduction of uniformly labeled (13)c-lutein and in vivo dosing in non-human primates.lutein is a xanthophyll abundant in nature and most commonly present in the human diet through consumption of leafy green vegetables. with zeaxanthin and meso-zeaxanthin, lutein is a component of the macular pigment of the retina, where it protects against photooxidation and age-related macular degeneration. recent studies have suggested that lutein may positively impact cognition throughout the lifespan, but outside of the retina, the deposition, metabolism, and function(s) of lutein are poorly ...201727798119
genotyping-by-sequencing provides the discriminating power to investigate the subspecies of daucus carota (apiaceae).the majority of the subspecies of daucus carota have not yet been discriminated clearly by various molecular or morphological methods and hence their phylogeny and classification remains unresolved. recent studies using 94 nuclear orthologs and morphological characters, and studies employing other molecular approaches were unable to distinguish clearly many of the subspecies. fertile intercrosses among traditionally recognized subspecies are well documented. we here explore the utility of single ...201627793080
characterising genes associated with flowering time in carrot (daucus carota l.) using transcriptome analysis.carrot is generally regarded as a biennial plant with an obligatory vernalization requirement. early spring cultivation makes plants vulnerable to premature bolting, which results in a loss of commercial value. however, our knowledge of flowering time genes and flowering mechanisms in carrot remain limited. bolting behavior of d. carota ssp. carota 'songzi', a wild species sensitive to flower induction by vernalization and photoperiod, and orange cultivar 'amsterdam forcing', and their offspring ...201727775866
effects of chrysosporum (aphanizomenon) ovalisporum extracts containing cylindrospermopsin on growth, photosynthetic capacity, and mineral content of carrots (daucus carota).natural toxins produced by freshwater cyanobacteria, such as cylindrospermopsin, have been regarded as an emergent environmental threat. despite the risks for food safety, the impact of these water contaminants in agriculture is not yet fully understood. carrots (daucus carota) are root vegetables, extensively consumed worldwide with great importance for human nourishment and economy. it is, therefore, important to evaluate the possible effects of using water contaminated with cyanotoxins on car ...201727770233
assessing the impact of geogenic chromium uptake by carrots (daucus carota) grown in asopos river basin.a methodology was developed to assess the impact of geogenic origin hexavalent chromium uptake by carrots, and the risk of human consumption of carrots grown in asopos river basin in greece. a field scale experiment was conducted with carrots cultivated in treatment plots, with and without compost amendment, in order to assess the impact of carbon in the mobility and uptake of chromium by plants. the results suggested that there is a trend for chromium mobilization and uptake in the surface and ...201727764685
analysis of the use of microcystin-contaminated water in the growth and nutritional quality of the root-vegetable, daucus carota.toxic cyanobacterial blooms are often observed in freshwaters and may reflect the increased eutrophication of these environments and alterations in climate. cyanotoxins, such as microcystins (mcs), are an effective threat to many life forms, ranging from plants to humans. despite the research conducted to date on cyanotoxins, the risks associated to the use of contaminated water in agriculture require further elucidation. to tackle this aim, a research was conducted with the root-vegetable daucu ...201727752954
profiling of the terpene metabolome in carrot fruits of wild (daucus carota l. ssp. carota) accessions and characterization of a geraniol synthase.fruits from wild carrot (daucus carota l. ssp. carota) have been used for medicinal purposes since ancient times. the oil of its seeds, with their abundant monoterpenes and sesquiterpenes, has drawn attention in recent years because of its potential pharmaceutical application. a combined chemical, biochemical, and molecular study was conducted to evaluate the differential accumulation of terpene volatiles in carrot fruits of wild accessions. this work reports a similarity-based cloning strategy ...201627673494
patterns of gene flow between crop and wild carrot, daucus carota (apiaceae) in the united states.studies of gene flow between crops and their wild relatives have implications for both management practices for cultivation and understanding the risk of transgene escape. these types of studies may also yield insight into population dynamics and the evolutionary consequences of gene flow for wild relatives of crop species. moreover, the comparison of genetic markers with different modes of inheritance, or transmission, such as those of the nuclear and chloroplast genomes, can inform the relativ ...201627603516
anti-inflammatory potential of black carrot (daucus carota l.) polyphenols in a co-culture model of intestinal caco-2 and endothelial ea.hy926 cells.the present study was developed to determine the ability of polyphenol-rich black carrot and its by-products, i.e., peel and pomace, to modulate the inflammatory response in tumor necrosis factor α (tnf-α) treated endothelial cells after gastrointestinal digestion and in a co-culture of intestinal caco-2 and endothelial ea.hy926 cell model.201727561918
carotenoid biosynthesis in daucus carota.carrot (daucus carota) is one of the most important vegetable cultivated worldwide and the main source of dietary provitamin a. contrary to other plants, almost all carrot varieties accumulate massive amounts of carotenoids in the root, resulting in a wide variety of colors, including those with purple, yellow, white, red and orange roots. during the first weeks of development the root, grown in darkness, is thin and pale and devoid of carotenoids. at the second month, the thickening of the root ...201627485223
enhanced accumulation of phytosterols and phenolic compounds in cyclodextrin-elicited cell suspension culture of daucus carota.in this work, suspension-cultured cells of daucus carota were used to evaluate the effect of β-cyclodextrins on the production of isoprenoid and phenolic compounds. the results showed that the phytosterols and phenolic compounds were accumulated in the extracellular medium (15100μgl(-1) and 477.46μgl(-1), respectively) in the presence of cyclodextrins. unlike the phytosterol and phenolic compound content, β-carotene (1138.03μgl(-1)), lutein (25949.54μgl(-1)) and α-tocopherol (8063.82μgl(-1)) chl ...201627457992
quantifying biochemical quality parameters in carrots (daucus carota l.) - ft-raman spectroscopy as efficient tool for rapid metabolite profiling.application of ft-raman spectroscopy for simultaneous quantification of carotenoids, carbohydrates, polyacetylenes and phenylpropanoids with high bioactive potential was investigated in storage roots of daucus carota. within single ft-raman experiment carbohydrates, carotenoids, and polyacetylenes could be reliably quantified with high coefficients of determination of r(2)>0.91. the most abundant individual representatives of each compound class could be quantified with comparably high quality r ...201627374560
fractionation of plant bioactives from black carrots (daucus carota subspecies sativus varietas atrorubens alef.) by adsorptive membrane chromatography and analysis of their potential anti-diabetic activity.black and purple carrots have attracted interest as colored extracts for coloring food due to their high content of anthocyanins. this study aimed to investigate the polyphenol composition of black carrots. particularly, the identification and quantification of phenolic compounds of the variety deep purple carrot (dpc), which presents a very dark color, was performed by hplc-pda and hplc-esi-ms(n) analyses. the separation of polyphenols from a dpc xad-7 extract into an anthocyanin fraction (af) ...201627362825
exogenous gibberellin enhances secondary xylem development and lignification in carrot taproot.gibberellins (gas) are important growth regulators involved in plant development processes. however, limited information is known about the relationship between ga and xylogenesis in carrots. in this study, carrot roots were treated with ga3. the effects of applied ga3 on root growth, xylem development, and lignin accumulation were then investigated. results indicated that ga treatment dose-dependently inhibited carrot root growth. the cell wall significantly thickened in the xylem parenchyma. a ...201727335006
expression profiles of genes involved in jasmonic acid biosynthesis and signaling during growth and development of carrot.jasmonates (jas) are recognized as essential regulators in response to environmental stimuli and plant development. carrot is an apiaceae vegetable with great value and undergoes significant size changes over the course of plant growth. however, ja accumulation and its potential roles in carrot growth remain unclear. here, methyl ja (meja) levels and expression profiles of ja-related genes were analyzed in carrot roots and leaves at five developmental stages. meja levels in the roots and leaves ...201627325823
biofortification of carrot (daucus carota l.) with iodine and selenium in a field experiment.the low content of iodine (i) and selenium (se) forms available to plants in soil is one of the main causes of their insufficient transfer in the soil-plant-consumer system. their deficiency occurs in food in the majority of human and farm animal populations around the world. both elements are classified as beneficial elements. however, plant response to simultaneous fertilization with i and se has not been investigated in depth. the study (conducted in 2012-2014) included soil fertilization of ...201627303423
(1)h nmr-based metabolomics reveals a pedoclimatic metabolic imprinting in ready-to-drink carrot juices.carrots are usually consumed in their native form or processed into many different products. carrot juice is a popular beverage consumed throughout the world and is attracting increasing attention due to its nutritional value, being a natural source of bioactive compounds. ready-to-drink carrot juices produced in the same factory were analyzed by (1)h nuclear magnetic resonance (nmr) spectroscopy. the juices were made from carrot roots of the same cultivar grown in three different geographical a ...201627281439
β-2-himachalen-6-ol: a novel anticancer sesquiterpene unique to the lebanese wild carrot.daucus carota ssp. carota, also known as wild carrot, is a commonly used herb in lebanese folk medicine to treat several ailments including cancer. previous studies in our laboratories showed that the daucus carota oil extract (dcoe) and subsequent fractions exhibit antioxidant, anti-inflammatory and anti-cancer activities. in this study, we report the isolation and identification of the major compound responsible for the anti-cancer activity of dcoe along with the mechanism of action involved. ...201627240746
the carrot genome sequence brings colors out of the dark.the genome sequence of carrot (daucus carota l.) is the first completed for an apiaceae species, furthering knowledge of the evolution of the important euasterid ii clade. analyzing the whole-genome sequence allowed for the identification of a gene that may regulate the accumulation of carotenoids in the root.201627230684
transcriptome-based identification of genes revealed differential expression profiles and lignin accumulation during root development in cultivated and wild carrots.carrot root development associates lignin deposition and regulation. carrot is consumed worldwide and is a good source of nutrients. however, excess lignin deposition may reduce the taste and quality of carrot root. molecular mechanisms underlying lignin accumulation in carrot are still lacking. to address this problem, we collected taproots of wild and cultivated carrots at five developmental stages and analyzed the lignin content and characterized the lignin distribution using histochemical st ...201627160835
the complete chloroplast genome of north american ginseng, panax quinquefolius.we report complete nucleotide sequence of the panax quinquefolius chloroplast genome using next-generation sequencing technology. the genome size is 156 359 bp, including two inverted repeats (irs) of 52 153 bp, separated by the large single-copy (lsc 86 184 bp) and small single-copy (ssc 18 081 bp) regions. this cp genome encodes 114 unigenes (80 protein-coding genes, four rrna genes, and 30 trna genes), in which 18 are duplicated in the ir regions. overall gc content of the genome is 38.08%. a ...201627158867
a high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution.we report a high-quality chromosome-scale assembly and analysis of the carrot (daucus carota) genome, the first sequenced genome to include a comparative evolutionary analysis among members of the euasterid ii clade. we characterized two new polyploidization events, both occurring after the divergence of carrot from members of the asterales order, clarifying the evolutionary scenario before and after radiation of the two main asterid clades. large- and small-scale lineage-specific duplications h ...201627158781
carrot (daucus carota l.): nephroprotective against gentamicin-induced nephrotoxicity in rats.daucus carota l.(dc) commonly known as carrot, folkorically used as ethnomedicine to treat nephrosis and other urinary disorders. hence, the present study was aimed to investigate the nephroprotective effects of ethanolic root extract of dc against gentamicin-induced nephrotoxicity in albino wistar rats.201727127313
does origin always matter? evaluating the influence of nonlocal seed provenances for ecological restoration purposes in a widespread and outcrossing plant species.for restoration purposes, nature conservation generally enforces the use of local seed material based on the "local-is-best" (lib) approach. however, in some cases recommendations to refrain from this approach have been made. here we test if a common widespread species with no obvious signs of local adaptation may be a candidate species for abandoning lib during restoration. using 10 microsatellite markers we compared population genetic patterns of the generalist species daucus carota in indigen ...201527069613
a novel β-carotene-associated carrot (daucus carota l.) pectic polysaccharide.carrot (daucus carota l.) is a nutritional source with enriched β-carotene (0.01%) and pectins (0.8%). although studies have highlighted the association between β-carotene and carrot pectic polysaccharide (crpp), the precise binding of β-carotene to crpp and its biological implications were not yet clearly understood. the aim of the present study is to report for the first time a natural carbohydrate polymer-crpp-with associated β-carotene.201727068881
residual tembotrione and atrazine in carrot.carrot (daucus carota l.) is a vegetable crop that is grown throughout the year across various regions of brazil in rotation or in succession to other cultures. herbicide residual effect has emerged as a concern, because of the possibility of carryover. thus, the objective of this study was to evaluate the effect of tembotrione and atrazine residues - in mixture and isolated - on carrot planted in succession to corn. the experiment was designed in randomized blocks with five replications. treatm ...201627052932
projected dietary intake of zinc, copper, and cerium from consumption of carrot (daucus carota) exposed to metal oxide nanoparticles or metal ions.the expanding production and use of engineered nanomaterials (enms) have raised concerns about the potential risk of those materials to food safety and human health. in a prior study, the accumulation of zn, cu, and ce from zno, cuo, or ceo2, respectively, was examined in carrot (daucus carota l.) grown in sand culture in comparison to accumulation from exposure to equivalent concentrations of ionic zn(2+), cu(2+), or ce(4+). the fresh weight concentration data for peeled and unpeeled carrots we ...201626941758
increased nicotiana tabacum fitness through positive regulation of carotenoid, gibberellin and chlorophyll pathways promoted by daucus carota lycopene β-cyclase (dclcyb1) expression.carotenoids, chlorophylls and gibberellins are derived from the common precursor geranylgeranyl diphosphate (ggpp). one of the enzymes in carotenoid biosynthesis is lycopene β-cyclase (lcyb) that catalyzes the conversion of lycopene into β-carotene. in carrot, dclcyb1 is essential for carotenoid synthesis in the whole plant. here we show that when expressed in tobacco, increments in total carotenoids, β-carotene and chlorophyll levels occur. furthermore, photosynthetic efficiency is enhanced in ...201626893492
ultraviolet-c light effect on physicochemical, bioactive, microbiological, and sensorial characteristics of carrot (daucus carota) beverages.the aim of this research was to evaluate the effect of ultraviolet-c light on physicochemical, bioactive, microbial, and sensory characteristics of carrot beverages. beverages were formulated with different concentrations of carrot juice (60, 80, and 100% [v/v]) and treated with ultraviolet-c light at different flow rates (0, 0.5, 3.9, and 7.9 ml s(-1)) and times (5, 10, 15, 20, and 30 min), equivalent to ultraviolet-c dosages of 13.2, 26.4, 39.6, 52.8, and 79.2 j cm(-2) total soluble solids, ph ...201626893153
stress-induced accumulation of dcaox1 and dcaox2a transcripts coincides with critical time point for structural biomass prediction in carrot primary cultures (daucus carota l.).stress-adaptive cell plasticity in target tissues and cells for plant biomass growth is important for yield stability. in vitro systems with reproducible cell plasticity can help to identify relevant metabolic and molecular events during early cell reprogramming. in carrot, regulation of the central root meristem is a critical target for yield-determining secondary growth. calorespirometry, a tool previously identified as promising for predictive growth phenotyping has been applied to measure th ...201626858746
combined effect of starch/montmorillonite coating and passive map in antioxidant activity, total phenolics, organic acids and volatile of fresh-cut carrots.this work evaluates fresh-cut carrots (fcc) coated with montmorillonite (mmt) subjected to passive modified atmosphere packaging. carrots were sanitized, cooled, peeled and sliced. half of the fcc were coated with mmt nanoparticle film and the other half were not. all fccs were packed in a polypropylene rigid tray, covered with a polypropylene rigid lid or sealed with polyethylene + propylene film, in four treatments (rl, rigid lid; rlc, rigid lid + coating; st, sealed tray; stc, sealed tray + c ...201626857136
assessment of antisecretory, gastroprotective, and in-vitro antacid potential of daucus carota in experimental rats.in indo china, carrots have been reported to regulate the functions of the stomach and intestines. the objective of the present investigation was to unravel the therapeutic potential of 50% ethanol extract from daucus carota roots (edc) on antisecretory, gastroprotective, and in vitro antacid capacity using experimental rats.201526835241
effect of carrot intake in the prevention of gastric cancer: a meta-analysis.gastric cancer is the third leading cause of cancer-related mortality, with the incidence and mortality being higher in men than in women. various studies have shown that eating carrots may play a major role in the prevention of gastric cancer. we conducted a meta-analysis to determine the relationship between carrot consumption and gastric cancer.201526819805
evaluation of native plant flower characteristics for conservation biological control of prays oleae.several studies have shown that manipulating flowering weeds within an agroecosystem can have an important role in pest control by natural enemies, by providing them nectar and pollen, which are significant sources of nutrition for adults. the aim of this study was to assess if the olive moth, prays oleae (bernard, 1788) (lepidoptera: praydidae), and five of its main natural enemies, the parasitoid species chelonus elaeaphilus silvestri (hymenoptera: braconidae), apanteles xanthostigma (haliday) ...201626780918
differential pigment accumulation in carrot leaves and roots during two growing periods.carotenoids are important secondary metabolites involved in plant growth and nutritional quality of vegetable crops. these pigments are highly accumulated in carrot root, but knowledge about the impact of environmental factors on their accumulation is limited. the purpose of this work was to investigate the impact of environmental variations on carotenoid accumulation in carrot leaves and roots. in this work, carrots were grown during two contrasting periods to maximize bioclimatic differences. ...201626752004
a novel in vivo β carotene biosensor for heavy metals detection.a novel whole cell-based biosensor was constructed using daucus carota cell as a biological component, with in vivo β-carotene as a reporter group. in this biosensor, the cells were immobilized using agarose gel, and change in in vivo β-carotene, before and after exposure of cells to heavy metals was noted using spectrophotometer at λ = 450 nm. biosensor was found to operate at its optimum condition using cells from day-14 culture with density of 8 x 10(5) cells/cuvette, and immobilized with 0.5 ...201526688961
combined effect of water loss and wounding stress on gene activation of metabolic pathways associated with phenolic biosynthesis in carrot.the application of postharvest abiotic stresses is an effective strategy to activate the primary and secondary metabolism of plants inducing the accumulation of antioxidant phenolic compounds. in the present study, the effect of water stress applied alone and in combination with wounding stress on the activation of primary (shikimic acid) and secondary (phenylpropanoid) metabolic pathways related with the accumulation of phenolic compound in plants was evaluated. carrot (daucus carota) was used ...201526528305
eating quality of carrots (daucus carota l.) grown in one conventional and three organic cropping systems over three years.the eating quality of carrots (daucus carota l.) was investigated to evaluate the impact of cropping systems (one conventional and three organic systems) and growing years (2007, 2008, and 2009) on root size, chemical composition, and sensory quality. the content of dry matter, sugars, polyacetylenes, and terpenes as well as the sensory quality and root size were related to the climate during the three growing years. a higher global radiation and a higher temperature sum in 2009 as compared to 2 ...201526513153
bioactive c₁₇-polyacetylenes in carrots (daucus carota l.): current knowledge and future perspectives.c17-polyacetylenes (pas) are a prominent group of oxylipins and are primarily produced by plants of the families apiaceae, araliaceae, and asteraceae, respectively. recent studies on the biological activity of polyacetylenes have indicated their potential to improve human health due to anticancer, antifungal, antibacterial, anti-inflammatory, and serotogenic effects. these findings suggest targeting vegetables with elevated levels of bisacetylenic oxylipins, such as falcarinol, by breeding studi ...201526451696
redirection of metabolite biosynthesis from hydroxybenzoates to volatile terpenoids in green hairy roots of daucus carota.a metabolic shift in green hairy root cultures of carrot from phenylpropanoid/benzenoid biosynthesis toward volatile isoprenoids was observed when compared with the metabolite profile of normal hairy root cultures. hairy roots cultures of daucus carota turned green under continuous illumination, while the content of the major phenolic compound p-hydroxybenzoic acid (p-hba) was reduced to half as compared to normal hairy roots cultured in darkness. p-hydroxybenzaldehyde dehydrogenase (hbd) activi ...201626403287
effect of hot water blanching time and drying temperature on the thin layer drying kinetics of and anthocyanin degradation in black carrot (daucus carota l.) shreds.this study was conducted to investigate the effect of blanching treatment (98 °c for 3 and 6 min) and air drying temperature of 40, 50 and 60 °c on the thin layer drying characteristics such as drying time, drying rate constant, effective moisture diffusivity and activation energy, as well as on anthocyanin content of black carrot shreds. it was observed that drying temperature affected the drying rate but blanching did not have an effect on drying time. three thin layer drying models, i.e. page ...201527904364
the complete chloroplast genome of north american ginseng, panax quinquefolius.we report complete nucleotide sequence of the panax quinquefolius chloroplast genome using next-generation sequencing technology. the genome size is 156 359 bp, including two inverted repeats (irs) of 52 153 bp, separated by the large single-copy (lsc 86 184 bp) and small single-copy (ssc 18 081 bp) regions. this cp genome encodes 114 unigenes (80 protein-coding genes, four rrna genes, and 30 trna genes), in which 18 are duplicated in the ir regions. overall gc content of the genome is 38.08%. a ...201526309073
pervasive mitochondrial sequence heteroplasmy in natural populations of wild carrot, daucus carota spp. carota l.exceptions to the generally accepted rules that plant mitochondrial genomes are strictly maternally inherited and that within-individual sequence diversity in those genomes, i.e., heteroplasmy, should be minimal are becoming increasingly apparent especially with regard to sequence-level heteroplasmy. these findings raise questions about the potential significance of such heteroplasmy for plant mitochondrial genome evolution. still studies quantifying the amount and consequences of sequence heter ...201526295342
the complete chloroplast genome of north american ginseng, panax quinquefolius.we report complete nucleotide sequence of the panax quinquefolius chloroplast genome using next-generation sequencing technology. the genome size is 156 359 bp, including two inverted repeats (irs) of 52 153 bp, separated by the large single-copy (lsc 86 184 bp) and small single-copy (ssc 18 081 bp) regions. this cp genome encodes 114 unigenes (80 protein-coding genes, four rrna genes, and 30 trna genes), in which 18 are duplicated in the ir regions. overall gc content of the genome is 38.08%. a ...201526273918
bioaccessibility of polyphenols from plant-processing byproducts of black carrot (daucus carota l.).plant-processing byproducts of black carrot represent an important disposal problem for the industry; however, they are also promising sources of polyphenols, especially anthocyanins. the present study focused on the changes in polyphenols from black carrot, peel, and pomace during in vitro gastrointestinal digestion. total phenolic content (tpc), total monomeric anthocyanin content (tmac), and total antioxidant capacity (tac) were determined using spectrophotometric methods, whereas identificat ...201626262673
evaluation of combined disinfection methods for reducing escherichia coli o157:h7 population on fresh-cut vegetables.most current disinfection strategies for fresh-cut industry are focused on the use of different chemical agents; however, very little has been reported on the effectiveness of the hurdle technology. the effect of combined decontamination methods based on the use of different sanitizers (peroxyacetic acid and chlorine dioxide) and the application of pressure (vacuum/positive pressure) on the inactivation of the foodborne pathogen e. coli o157:h7 on fresh-cut lettuce (lactuca sativa) and carrots ( ...201526213954
analysis of the thermal degradation of the individual anthocyanin compounds of black carrot (daucus carota l.): a new approach using high-resolution proton nuclear magnetic resonance spectroscopy.the black carrot dye is a mixture of cyanidin molecules, the nuclear magnetic resonance (nmr) spectrum of which shows a highly overlapped aromatic region. in this study, the (1)h nmr (800 mhz) aromatic chemical shifts of the mixture were fully assigned by overlaying them with the characterized (1)h nmr chemical shifts of the separated compounds. the latter were isolated using reverse-phase high-performance liquid chromatography (rp-hplc), and their chemical shifts were identified using (1)h and ...201526160425
espresso coffee residues as a nitrogen amendment for small-scale vegetable production.espresso coffee grounds constitute a residue which is produced daily in considerable amounts, and is often pointed out as being potentially interesting for plant nutrition. two experiments (incubations and field experiments) were carried out to evaluate the potential nitrogen (n) and phosphorus (p) supply for carrot (daucus carota l.), spinach (spinacea oleracea l.) and lettuce (lactuca sativa l.) nutrition.201526123995
daucus carota pentane/diethyl ether fraction inhibits motility and reduces invasion of cancer cells.daucus carota (dc) is a herb used in folklore medicine in lebanon to treat numerous diseases including cancer. recent studies in our laboratory on dc oil and its fractions revealed potent anticancer activities in vitro and in vivo. the present study aims to investigate the effect of the most potent dc fraction, pentane/diethyl ether (50:50), on lung, skin, breast and glioblastoma cancer cell motility and invasion. upon treatment, a pronounced decrease in cancer cell motility was observed in the ...201426088465
influence of different processing and storage conditions on in vitro bioaccessibility of polyphenols in black carrot jams and marmalades.black carrot is indicated to play an important role in nutrition, as it comprises a variety of health-promoting components, including polyphenols. the objective of the present study was to monitor the stability of total phenolics, antioxidant capacity and phenolic acids in black carrot jams and marmalades after processing, storage and in vitro gastrointestinal digestion. total phenolic content and antioxidant capacity were determined using spectrophotometric methods, whereas phenolic acids were ...201525976794
polyacetylenes from carrots (daucus carota) improve glucose uptake in vitro in adipocytes and myotubes.a dichloromethane (dcm) extract of carrot roots was found to stimulate insulin-dependent glucose uptake (gu) in adipocytes in a dose dependent manner. bioassay-guided fractionation of the dcm extract resulted in the isolation of the polyacetylenes falcarinol and falcarindiol. both polyacetylenes were able to significantly stimulate basal and/or insulin-dependent gu in 3t3-l1 adipocytes and porcine myotube cell cultures in a dose-dependent manner. falcarindiol increased peroxisome proliferator-ac ...201525970571
regulation of ascorbic acid biosynthesis and recycling during root development in carrot (daucus carota l.).ascorbic acid (asa), also known as vitamin c, is an essential nutrient in fruits and vegetables. the fleshy root of carrot (daucus carota l.) is a good source of asa for humans. however, the metabolic pathways and molecular mechanisms involved in the control of asa content during root development in carrot have not been elucidated. to gain insights into the regulation of asa accumulation and to identify the key genes involved in the asa metabolism, we cloned and analyzed the expression of 21 rel ...201525956452
actinorugispora endophytica gen. nov., sp. nov., an actinomycete isolated from daucus carota.an actinomycete strain, designated yim 690008t, was isolated from daucus carota collected from south korea and its taxonomic position was investigated by using a polyphasic approach. the strain grew well on most media tested and no diffusible pigment was produced. the aerial mycelium formed wrinkled single spores and short spore chains, some of which were branched. the whole-cell hydrolysates contained meso-diaminopimelic acid, glucose, mannose, ribose, galactose and rhamnose. the predominant me ...201525948617
identification and characterization of terpene synthases potentially involved in the formation of volatile terpenes in carrot (daucus carota l.) roots.plants produce an excess of volatile organic compounds, which are important in determining the quality and nutraceutical properties of fruit and root crops, including the taste and aroma of carrots (daucus carota l.). a combined chemical, biochemical, and molecular study was conducted to evaluate the differential accumulation of volatile terpenes in a diverse collection of fresh carrots (d. carota l.). here, we report on a transcriptome-based identification and functional characterization of two ...201525924989
transfer model of lead in soil-carrot (daucus carota l.) system and food safety thresholds in soil.reliable empirical models describing lead (pb) transfer in soil-plant systems are needed to improve soil environmental quality standards. a greenhouse experiment was conducted to develop soil-plant transfer models to predict pb concentrations in carrot (daucus carota l.). soil thresholds for food safety were then derived inversely using the prediction model in view of the maximum allowable limit for pb in food. the 2 most important soil properties that influenced carrot pb uptake factor (ratio o ...201525904232
design and application of a biphasic system that enhances productivity of daucus carota-catalyzed asymmetric reduction.to develop a biphasic system for use in plant tissue-mediated biotransformations to overcome the low water solubilities of substrates and inhibitory effects of products.201525893325
antioxidant and hepatoprotective activities of the oil fractions from wild carrot (daucus carota ssp. carota).wild carrot, daucus carota l. ssp. carota (apiacae), is widely distributed throughout the world and has various uses in traditional medicine in lebanon.201525856705
influence of cultivar and harvest year on the volatile profiles of leaves and roots of carrots (daucus carota spp. sativus hoffm.).the focus of the present work centers on the diversity of volatile patterns of carrots. in total 15 main volatiles were semiquantified in leaves and roots using isolation by headspace solid phase microextraction followed by gas chromatography with fid and ms detection. significant differences in the main number of compounds were detected between the cultivars as well as the years. genotype-environment interactions (g × e) are discussed. the most abundant metabolites, β-myrcene (leaves) and terpi ...201525797828
modeling the transfer of arsenic from soil to carrot (daucus carota l.)--a greenhouse and field-based study.reliable empirical models describing arsenic (as) transfer in soil-plant systems are needed to estimate the human as burden from dietary intake. a greenhouse experiment was conducted in parallel with a field trial located at three sites through china to develop and validate soil-plant transfer models to predict as concentrations in carrot (daucus carota l.). stepwise multiple linear regression relationships were based on soil properties and the pseudo total (aqua regia) or available (0.5 m nahco ...201525750050
cross-talk between signaling pathways: the link between plant secondary metabolite production and wounding stress response.plants subjected to wounding stress produce secondary metabolites. several of these metabolites prevent chronic diseases and can be used as colorants, flavors, and as antimicrobials. this wound-induced production of plant secondary metabolites is mediated by signaling-molecules such as reactive oxygen species (ros), ethylene (et) and jasmonic acid (ja). however, their specific role and interactions that modulate the wound-respond in plants is not fully understood. in the present study, a subtrac ...201525712739
two new eudesmane-type glucopyranosides from the fruits of daucus carota l.two new eudesmane-type glucopyranosides have been isolated from the fruits of daucus carota l. on the basis of their spectroscopic and chemical evidence, the new compounds were elucidated as daucucarotol-10-o-β-d-glucopyranoside (1) and decahydro-7-[(2-o-β-d-glucopyranosyl)-isopropyl]-1β,4aα-dimethyl-(1α,4α,8aβ)-naphthalenetriol (2). compounds 1 and 2 showed moderate antitumour activity against human eca-109 and gave ic50 values of 23.22 and 26.76 μm, respectively.201525686649
wild carrot oil extract is selectively cytotoxic to human acute myeloid leukemia cells.in this study, we used daucus carota oil extract (dcoe) to target acute myeloid leukemia (aml) cells. all the aml cell lines tested were sensitive to the extract while peripheral mononuclear cells were not. analysis of mechanism of cell death showed an increase in cells positive for annexinv and for active caspases, indicating that dcoe induces apoptotic cell death in aml. inhibition of the mapk pathway decreased sensitivity of aml cells to dcoe, indicating that cytotoxicity may be dependent on ...201525684522
removal notice to "protective effect of daucus carota against uv-dmba induced skin cancer in mice" [chem. biol. interact. 219 (2014) 48-56]. 201425606606
daucus carota pentane-based fractions suppress proliferation and induce apoptosis in human colon adenocarcinoma ht-29 cells by inhibiting the mapk and pi3k pathways.daucus carota l. ssp. carota (apiacea, wild carrot, queen anne's lace) has been used in folk medicine throughout the world and recently was shown to possess anticancer and antioxidant activities. this study aims to determine the anticancer activity of the pentane fraction (f1) and the 1:1 pentane:diethyl ether fraction (f2) of the daucus carota oil extract (dcoe) against human colon adenocarcinoma cell lines (ht-29 and caco-2). treatment of cells with various concentrations of f1 or f2 fractions ...201525599142
impact and correlation of environmental conditions on pollen counts in karachi, pakistan.a quantitative and qualitative survey of airborne pollen was performed in the city of karachi, and the pollen counts were correlated with different climatic conditions. the aim of the study was to determine the possible effect of meteorological factors on airborne pollen distribution in the atmosphere of karachi city. pollen sampling was carried out by using burkard spore trap for the period of august 2009 to july 2010, and a total of 2,922 pollen grains/m(3) were recorded. in this survey, 22 po ...201525530143
composition and (in)homogeneity of carotenoid crystals in carrot cells revealed by high resolution raman imaging.three categories of roots differing in both β/α-carotene ratio and in total carotenoid content were selected based on hplc measurements: high α- and β-carotene (hαhβ), low α- and high β-carotene (lαhβ), and low α- and low β-carotene (lαlβ). single carotenoid crystals present in the root cells were directly measured using high resolution raman imaging technique with 532nm and 488nm lasers without compound extraction. crystals of the hαhβ root had complex composition and consisted of β-carotene ac ...201525459698
phenotyping carrot (daucus carota l.) for yield-determining temperature response by calorespirometry.calorespirometric measurements proved to be useful for phenotyping temperature response in terms of optimum temperatures for growth and low temperature limits for growth respiration in diverse carrot genotypes. high and low-temperature tolerance is an important trait in many breeding programs, but to date, improvement strategies have had limited success. developing new, cost efficient and reliable screening tools to identify and select the most tolerant crop plant genotypes is necessary to assis ...201525380771
morphometrics of daucus (apiaceae): a counterpart to a phylogenomic study.molecular phylogenetics of genome-scale data sets (phylogenomics) often produces phylogenetic trees with unprecedented resolution. a companion phylogenomics analysis of daucus using 94 conserved nuclear orthologs supported many of the traditional species but showed unexpected results that require morphological analyses to help interpret them in a practical taxonomic context.201425366864
effect of acidification on carrot (daucus carota) juice cloud stability.effects of acidity on cloud stability in pasteurized carrot juice were examined over the ph range of 3.5-6.2. cloud sedimentation, particle diameter, and ζ potential were measured at each ph condition to quantify juice cloud stability and clarification during 3 days of storage. acidification below ph 4.9 resulted in a less negative ζ potential, an increased particle size, and an unstable cloud, leading to juice clarification. as the acidity increased, clarification occurred more rapidly and to a ...201425354298
mapping genes governing flower architecture and pollen development in a double mutant population of carrot.a linkage map of carrot (daucus carota l.) was developed in order to study reproductive traits. the f2 mapping population derived from an initial cross between a yellow leaf (yel) chlorophyll mutant and a compressed lamina (cola) mutant with unique flower defects of the sporophytic parts of male and female organs. the genetic map has a total length of 781 cm and included 285 loci. the length of the nine linkage groups (lgs) ranged between 65 and 145 cm. all lgs have been anchored to the referenc ...201425339960
new insights into domestication of carrot from root transcriptome analyses.understanding the molecular basis of domestication can provide insights into the processes of rapid evolution and crop improvement. here we demonstrated the processes of carrot domestication and identified genes under selection based on transcriptome analyses.201425311557
daucus carota pentane-based fractions arrest the cell cycle and increase apoptosis in mda-mb-231 breast cancer cells.daucus carota l.ssp.carota (wild carrot), an herb used in folk medicine worldwide, was recently demonstrated to exhibit anticancer activity. in this study we examined the anticancer effect of daucus carota oil extract (dcoe) fractions on the human breast adenocarcinoma cell lines mda-mb-231 and mcf-7 and clarified the mechanism of action.201425300932
effect of β-lactam antibiotics on plant regeneration in carrot protoplast cultures.protoplasts of three carrot cultivars were isolated from in vitro-grown plantlets by overnight incubation in an enzyme mixture composed of 1% (w/v) cellulase onozuka r-10 and 0.1% (w/v) pectolyase y-23. after cell immobilization in modified thin alginate layers, three types of β-lactam antibiotics (cefotaxime, carbenicillin, or timentin) at five different concentrations (100, 200, 300, 400, or 500 mg l(-1)) were added to the culture medium. in 20-d-old cultures, a different number of cell coloni ...201425298730
investigating the performance of in situ quantitative nuclear magnetic resonance analysis and applying the method to determine the distribution of saccharides in various parts of carrot roots (daucus carota l.).in order to explore the performance of the analytical method called in situ quantitative nuclear magnetic resonance spectroscopy - is q nmr - the distribution of glucose, fructose and sucrose in various parts of a carrot root (daucus carota l.) - primary xylem, secondary xylem, phloem, cortex; top part and lower part - was determined. the influence on the quality of spectra of drying samples before analysis was studied, as well as the influence of the length of strips of tissue used in analysis. ...201525281111
transcript profiling of structural genes involved in cyanidin-based anthocyanin biosynthesis between purple and non-purple carrot (daucus carota l.) cultivars reveals distinct patterns.carrots (daucus carota l.) are among the 10 most economically important vegetable crops grown worldwide. purple carrot cultivars accumulate rich cyanidin-based anthocyanins in a light-independent manner in their taproots whereas other carrot color types do not. anthocyanins are important secondary metabolites in plants, protecting them from damage caused by strong light, heavy metals, and pathogens. furthermore, they are important nutrients for human health. molecular mechanisms underlying antho ...201425269413
carrotdb: a genomic and transcriptomic database for carrot.carrot (daucus carota l.) is an economically important vegetable worldwide and is the largest source of carotenoids and provitamin a in the human diet. given the importance of this vegetable to humans, research and breeding communities on carrot should obtain useful genomic and transcriptomic information. the first whole-genome sequences of 'dc-27' carrot were de novo assembled and analyzed. transcriptomic sequences of 14 carrot genotypes were downloaded from the sequence read archive (sra) data ...201425267795
in vitro bioaccessibility of β-carotene, ca, mg and zn in landrace carrots (daucus carota, l.).four landrace carrots ("becaria", "crs", "gonzález" and "rodríguez") and two marketable cultivars (kuroda and brasilia), raw and steamed, were characterised by the total content of β-carotene ca, mg and zn, in vitro bioaccessibility and by colour and were evaluated to determine the effect of particle size in nutrient bioaccessibility. steaming increased the content of β-carotene extracted from "crs" and brasilia (29% and 75%) and decreased the content of β-carotene extracted from "crs" by 23% in ...201525053069
essential-oil composition of daucus carota ssp. major (pastinocello carrot) and nine different commercial varieties of daucus carota ssp. sativus fruits.the chemical composition of the essential oils obtained by hydrodistillation from the pastinocello carrot, daucus carota ssp. major (vis.) arcang. (flowers and achenes), and from nine different commercial varieties of d. carota l. ssp. sativus (achenes) was investigated by gc/ms analyses. selective breeding over centuries of a naturally occurring subspecies of the wild carrot, d. carota l. ssp. sativus, has produced the common garden vegetable with reduced bitterness, increased sweetness, and mi ...201425044588
sensory and health properties of steamed and boiled carrots (daucus carota ssp. sativus).this study examined the influences of domestic processing conditions applied by consumers on firmness, colour and amount of phytochemicals and liking and sensory attributes intensity rating of carrots. the aim was to identify a cooking method and time that yields carrots with higher amount of β-carotene while maintaining consumer liking. instrumentally measured firmness and colour showed comparable degradation trends between cooking methods. while boiling showed a significant decrease in the amo ...201424964285
an improved protocol for carrot haploid and doubled haploid plant production using induced parthenogenesis and ovule excision in vitro.in this work, we describe an improved protocol for induced parthenogenesis and ovule culture of carrot (daucus carota l.). the effects of pollination with parsley pollen and/or 2,4-dichlorophenoxyacetic acid (2,4-d) treatment on the stimulation of parthenogenesis were studied using heterozygous donor plants of 30 varieties and breeding populations of carrots. isolated ovules, cultured in vitro, enlarged and developed embryos or calli. the application of 2,4-d on pollinated flowers stimulated cal ...201424955000
characterization of centromeric histone h3 (cenh3) variants in cultivated and wild carrots (daucus sp.).in eukaryotes, centromeres are the assembly sites for the kinetochore, a multi-protein complex to which spindle microtubules are attached at mitosis and meiosis, thereby ensuring segregation of chromosomes during cell division. they are specified by incorporation of cenh3, a centromere specific histone h3 variant which replaces canonical histone h3 in the nucleosomes of functional centromeres. to lay a first foundation of a putative alternative haploidization strategy based on centromere-mediate ...201424887084
removed: protective effect of daucus carota against uv-dmba induced skin cancer in mice.this article has been removed: please see elsevier policy on article withdrawal (http://www.elsevier.com/locate/withdrawalpolicy). this article has been removed at the request of "due to a processing error during the review process, this paper was erroneously published online prematurely. the publisher apologizes to readers and the authors for any inconvenience caused."201424812028
mapping the variation of the carrot metabolome using 1h nmr spectroscopy and consensus pca.genetic variation is the most influential factor for carrot (daucus carota l.) composition. however, difference in metabolite content between carrot varieties has not been described by nmr, although primary metabolites are important for human health and sensory properties. the aim of the present study was to investigate the effect of genotype on carrot metabolite composition using a (1)h nmr-based metabolomics approach. after extraction using aqueous and organic solvents, 25 hydrophilic metaboli ...201424773156
induction of extracellular defense-related proteins in suspension cultured-cells of daucus carota elicited with cyclodextrins and methyl jasmonate.suspension cultured-cells (scc) of daucus carota were used to evaluate the effect of methyl jasmonate and cyclodextrins, separately or in combination, on the induction of defense responses, particularly the accumulation of pathogenesis-related proteins. a comparative study of the extracellular proteome (secretome) between control and elicited carrot scc pointed to the presence of amino acid sequences homologous to glycoproteins which have inhibitory activity against the cell-wall-degrading enzym ...201424589476
the efficacy of essential oils as natural preservatives in vegetable oil.the efforts for finding the natural preservatives with nontoxicity and nonirritancy have encouraged the scientists to research among the medicinal plants. the preservative efficacy of daucus carota, ferula gummosa, eugenium caryophyllata, oliveria decumbens, pelargonium graveolens, ziziphora tenuir, acorus calamus, and trachyspermum ammi essential oils on challenge test's pathogens and on pathogen's inoculated vegetable oil was evaluated by antimicrobial effectiveness test. carotol (46%), β-pine ...201424552253
diversity, genetic mapping, and signatures of domestication in the carrot (daucus carota l.) genome, as revealed by diversity arrays technology (dart) markers.carrot is one of the most economically important vegetables worldwide, but genetic and genomic resources supporting carrot breeding remain limited. we developed a diversity arrays technology (dart) platform for wild and cultivated carrot and used it to investigate genetic diversity and to develop a saturated genetic linkage map of carrot. we analyzed a set of 900 dart markers in a collection of plant materials comprising 94 cultivated and 65 wild carrot accessions. the accessions were attributed ...201424532979
intra-population genetic diversity of cultivated carrot (daucus carota l.) assessed by analysis of microsatellite markers.intra-population variation of 18 cultivated carrot (daucus carota l. ssp. sativus) populations of diverse origins was evaluated using codominant microsatellite (ssr) markers. using 27 genomic and est-derived ssr markers, 253 alleles were identified with a mean 9.4 alleles per marker. most of the alleles (60.5%) were rare i.e., with the frequency ≤ 0.05 while only 3.95% of alleles occurred with frequency > 0.6. est-derived ssr markers were less polymorphic than genomic ssr markers. differences in ...201324432327
effect of pectin methylesterase on carrot (daucus carota) juice cloud stability.to determine the effect of residual enzyme activity on carrot juice cloud, 0 to 1 u/g pectin methylesterase (pme) was added to pasteurized carrot juice. cloud stability and particle diameters were measured to quantify juice cloud stability and clarification for 56 days of storage. all levels of pme addition resulted in clarification; higher amounts had a modest effect in causing more rapid clarification, due to a faster increase in particle size. the cloud initially exhibited a trimodal distribu ...201424401030
genetic and phenological variation of tocochromanol (vitamin e) content in wild (daucus carota l. var. carota) and domesticated carrot (d. carota l. var. sativa).carrot roots (daucus carota l. var. sativa) produce tocochromanol compounds, collectively known as vitamin e. however, little is known about their types and amounts. here we determined the range and variation in types and amounts of tocochromanols in a variety of cultivated carrot accessions throughout carrot postharvest storage and reproductive stages and in wild-type roots (daucus carota l. var. carota). of eight possible tocochromanol compounds, we detected and quantified α-, and the combined ...201426504534
combating photooxidative stress in green hairy roots of daucus carota cultivated under light irradiation.the light-dependent generation of active oxygen species, which can disrupt normal metabolic process of plant, is termed as photo-oxidative stress. plants are equipped with enzymatic and non-enzymatic antioxidative defence system to reduce the effect of such stress. hairy root culture of daucus carota when cultivated under continuous illumination (250 μmol m(-2)s(-1)) turned green. to know the reason behind that and photo-oxidative stress response in green hairy roots, activities of several antio ...201424331434
sodium-hydrogen exchanger inhibitory potential of malus domestica, musa × paradisiaca, daucus carota, and symphytum officinale.the involvement of sodium-hydrogen exchangers (nhe) has been described in the pathophysiology of diseases including ischemic heart and brain diseases, cardiomyopathy, congestive heart failure, epilepsy, dementia, and neuropathic pain. synthetic nhe inhibitors have not achieved much clinical success; therefore, plant-derived phytoconstituents may be explored as nhe inhibitors.201424184990
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