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a nuclear protein associated with cell divisions in plants.a nuclear protein, present in carrot meristems and rapidly proliferating cultured cells of carrot (daucus carota l.) has been identified by the use of a monoclonal antibody (mab 21d7). by combining the techniques of two-dimensional polyacrylamide gel analysis and blotting separated proteins onto nitrocellulose sheets, it was shown that the antibody detected a single polypeptide of apparent molecular mass (m r) of 45000 and an isoelectric focusing point (pi) of 6.7. this protein was found by subc ...198824221561
the effects of gelatin on aggregation of protoplasts from higher plants.aggregation of protoplasts of allium fistulosum l., brassica chinensis l. and daucus carota l. was induced in great frequency with gelatin and early products of gelatin degradation. the optimal gelatin concentration was 2-5%. the aggregated protoplasts fused into groups and cell walls were formed around them in culture.197324458819
herbal remedies and functional foods used by cancer patients attending specialty oncology clinics in trinidad.cancer is a major disease worldwide, and many patients use complementary and alternative treatments. the purpose of this study was to identify the herbal remedies and functional foods used as complementary medicine by prostate, breast and colorectal cancer patients at speciality care facilities in trinidad. we also sought to determine how patients rated the efficacy of these modalities compared with conventional treatment.201627769229
predator-prey relationships on apiaceae at an organic farm.orius insidiosus (say) and o. pumilio (champion) were confirmed to be sympatric in north central florida as the major predators of the florida flower thrips, frankliniella bispinosa (morgan), on flowers of queen anne's lace, daucus carota l. and false queen anne's lace, ammi majus l. f. bispinosa was the predominant thrips observed on both flowers but colonized d. carota to a greater extent and earlier in the season than a. majus. despite differences in the abundance of f. bispinosa on the two p ...201222732606
molecular evolution of flavonoid dioxygenases in the family apiaceae.plant species of the family apiaceae are known to accumulate flavonoids mainly in the form of flavones and flavonols. three 2-oxoglutarate-dependent dioxygenases, flavone synthase or flavanone 3 beta-hydroxylase and flavonol synthase are involved in the biosynthesis of these secondary metabolites. the corresponding genes were cloned recently from parsley (petroselinum crispum) leaves. flavone synthase i appears to be confined to the apiaceae, and the unique occurrence as well as its high sequenc ...200515913674
selecting iodine-enriched vegetables and the residual effect of iodate application to soil.a greenhouse pot experiment was conducted to select vegetables for iodine uptake. the residual effect of iodate fertilization on the growth of and iodine uptake by spinach plants were also investigated. six vegetables, including leafy vegetables (pakchoi [brassica chinensis l.], spinach [spinacia oleracea l.]), tuber vegetables (onion [allium cepa l.]), shoot vegetables (water spinach [ipomoea aquatica forsk.], celery [apium graveolens l.]), and root vegetables (carrot [daucus carota var. sativa ...200415564656
accurate mass gc/lc-quadrupole time of flight mass spectrometry analysis of fatty acids and triacylglycerols of spicy fruits from the apiaceae family.the triacylglycerol (tag) structure and the regio-stereospecific distribution of fatty acids (fa) of seed oils from most of the apiaceae family are not well documented. the tag structure ultimately determines the final physical properties of the oils and the position of fas in the tag molecule affects the digestion; absorption and metabolism; and physical and technological properties of tags. fixed oils from the fruits of dill (anethum graveolens), caraway (carum carvi), cumin (cuminum cyminum), ...201526633337
enantioselective hydrolysis of bromo- and methoxy-substituted 1-phenylethanol acetates using carrot and celeriac enzymatic systems.enantioselective hydrolysis of bromo- and methoxy-substituted 1-phenylethanol acetates was conducted using comminuted carrot (daucus carota l.) and celeriac (apium graveolens l. var. rapaceum) roots. hydrolysis of the acetates led to alcohols, preferentially to r-(+)-enantiomers. efficiencies of both reactions - hydrolysis of the acetates with an electron-donating methoxy group and oxidation of the resulting alcohols - increased in the following order: ortho < meta < para. the presence of an ele ...201317708446
bioaccumulation of antimony and arsenic in vegetables and health risk assessment in the superlarge antimony-mining area, china.heavy metal pollution in soils caused by mining and smelting has attracted worldwide attention for its potential health risks to residents. this paper studies the concentrations and accumulations of sb and as in both soils and vegetables and the human health risks of sb and as in vegetables from xikuangshan (xks) sb mine, hunan, china. results showed that the soils were severely polluted by sb and as; sb and as have significant positive correlation. sb and as concentrations in vegetables were qu ...201526442167
 the contents of sterol derivatives (sterols + esters, glycosides and acylated glycosides) have been determined using thin layer chromatography in leaves and bulky and organs from carrots (daucus carota l.) potato (solanum tuberosum l.), onion (allium cepa l.) two varieties of radish (raphanus sativus l.) and gentiana purpurea l. plants. ether-soluble lipids of leaves contain 2.8-5.2%, those of storage tissue 3.1-8.9% sterol derivatives. the fractionation of lettuce (lactuca sativa l., burpee's r ...197011945480
release of carrot plasma membrane-associated phosphatidylinositol kinase by phospholipase a2 and activation by a 70 kda protein.plasma membranes were isolated from carrot (daucus carota l.) cells grown in suspension culture and treated with phospholipase a2 from snake or bee venom for 10 min. as a result of this treatment, phosphatidylinositol kinase activity was recovered in the soluble fraction. there was no detectable diacylglycerol kinase or phosphatidylinositol monophosphate kinase activity released from the membranes after the phospholipase a2 treatment. treating the plasma membranes with phospholipase c or d did n ...19921311960
expression pattern of the arabidopsis thaliana atep3/atchitiv endochitinase gene.the carrot (daucus carota l.) ep3 chitinase was shown to be essential for somatic embryo formation in a carrot mutant cell line. we identified the arabidopsis thaliana (l.) heynh. ortholog of the carrot ep3-3 chitinase gene, designated as atep3/atchitiv and analyzed its expression in arabidopsis by means of reverse transcription-polymerase chain reaction and promoter::beta-glucuronidase and luciferase fusions. as in carrot, the gene is expressed during somatic embryogenesis in "nursing" cells su ...200111525512
a novel k+ channel expressed in carrot roots with a low susceptibility toward metal ions.kdc1 is a novel k+-channel gene cloned from carrot roots, and which is also present in cultured carrot cells. we investigated the characteristics of the ionic current elicited in xenopus oocytes coinjected with kdc1 (k+-daucus carota 1) and kat1 (from arabidopsis thaliana) rna. expressed heteromeric channels displayed inward-rectifying potassium currents whose kinetics, voltage characteristics, and inhibition by metal ions depended on kdc1:kat1 ratios. at low kdc1:kat1 ratios, zn2+ inhibition of ...200111460927
differential contribution of the first two enzymes of the mep pathway to the supply of metabolic precursors for carotenoid and chlorophyll biosynthesis in carrot (daucus carota).carotenoids and chlorophylls are photosynthetic pigments synthesized in plastids from metabolic precursors provided by the methylerythritol 4-phosphate (mep) pathway. the first two steps in the mep pathway are catalyzed by the deoxyxylulose 5-phosphate synthase (dxs) and reductoisomerase (dxr) enzymes. while dxs has been recently shown to be the main flux-controlling step of the mep pathway, both dxs and dxr enzymes have been proven to be able to promote an increase in mep-derived products when ...201627630663
daucus carota l.--an old model for cell reprogramming gains new importance through a novel expansion pattern of alternative oxidase (aox) genes.the paper highlights daucus carota l. as an ideal model to complement plant stress research on arabidopsis thaliana l. recently, alternative oxidase (aox) is discussed as functional marker candidate for cell reprogramming upon stress. carrot is the most studied species for cell reprogramming and our current research reveals that it is the only one that has expanded both aox sub-family genes. we point to recently published, but not discussed results on conserved differences in the vicinity of the ...200919372042
distinct patterns of expression but similar biochemical properties of protein l-isoaspartyl methyltransferase in higher plants.protein l-isoaspartyl methyltransferase is a widely distributed repair enzyme that initiates the conversion of abnormal l-isoaspartyl residues to their normal l-aspartyl forms. here we show that this activity is expressed in developing corn (zea mays) and carrot (daucus carota var. danvers half long) plants in patterns distinct from those previously seen in winter wheat (triticum aestivum cv augusta) and thale cress (arabidopsis thaliana), whereas the pattern of expression observed in rice (oryz ...200111161058
daucus carota as a novel model to evaluate the effect of light on carotenogenic gene expression.carotenoids are synthesized in prokaryotic and eukaryotic organisms. in plants and algae, these lipophilic molecules possess antioxidant properties acting as reactive oxygen species scavengers and exert functional roles in hormone synthesis, photosynthesis, photomorphogenesis and in photoprotection. during the past decade almost all carotenogenic genes have been identified as a result of molecular, genetic and biochemical approaches utilizing arabidopsis thaliana as the model system. studies car ...200819399342
the zinc binding site of the shaker channel kdc1 from daucus carota.kdc1 is a voltage-dependent shaker-like potassium channel subunit cloned from daucus carota which produces conductive channels in xenopus oocytes only when coexpressed with other plant shaker potassium subunits, such as kat1 from arabidopsis thaliana. external zn(2+) determines a potentiation of the current mediated by the dimeric construct kdc1-kat1, which has been ascribed to zinc binding at a site comprising three histidines located at the s3-s4 (h161, h162) and s5-s6 (h224) linkers of kdc1. ...200817890387
[advanced study on serk genes family].somatic embryogenesis receptor-like kinase (serk) genes were identified in different plant species, such as daucus carota, arabidopsis thaliana and oryza sativa. studies of these genes showed that they have conserved structures and expression patterns. these genes are not only expressed in embryogenic tissues, but also in post-embryogenic development. they function in embryogenesis and reproductive development and defences in particular, related signal transduction pathways.200717650484
n-acetyl glutamate kinase from daucus carota suspension cultures: embryogenic expression profile, purification and characterization.in daucus carota, n-acetylglutamate-5-phosphotransferase (nagk; e.c. 2.7.2.8) specific activity was shown to correlate with the progression of somatic embryogenesis and was highest in the latter stages, where growth was most rapid. the enzyme was subsequently purified greater than 1200-fold using heat treatment, ammonium sulfate fractionation, gel filtration, anion exchange and dye ligand chromatography. carrot nagk was shown to have a subunit molecular weight of 31 kda and form a hexamer. the k ...200516289950
crosstalk between aba and auxin signaling pathways in roots of arabidopsis thaliana (l.) heynh.studies of abscisic acid (aba) and auxin have revealed that these pathways impinge on each other. the daucus carota (l.) dc3 promoter: uida (beta-glucuronidase: gus) chimaeric reporter (prodc3:gus) is induced by aba, osmoticum, and the auxin indole-3-acetic acid (iaa) in vegetative tissues of transgenic arabidopsis thaliana (l.) heynh. here, we describe the root tissue-specific expression of prodc3:gus in the aba-insensitive-2 (abi2-1), auxin-insensitive-1 (aux1), auxin-resistant-4 (axr4), and r ...200515889272
atltp1 luciferase expression during carrot somatic embryogenesis.the carrot (daucus carota l.) ep2 gene encodes a lipid transfer protein (ltp) which is expressed during protoderm formation in developing embryos. to develop a vital reporter system for gene expression during somatic embryo development a 1.1 kb fragment of the arabidopsis thaliana ltp1 promoter was fused to the firefly luciferase (luc) coding sequence. the atltp1 luciferase expression pattern in transformed carrot suspension cultures was identical to the expression pattern of the endogenous carr ...19979418058
male sterility in arabidopsis induced by overexpression of a myc5-srdx chimeric repressor.jasmonate hormone (ja) plays critical roles in both plant defense and reproductive development. arabidopsis thaliana plants deficient in ja-biosynthesis or -signaling are male-sterile, with defects in stamen and pollen development. myc2, myc3 and myc4 are jaz-interacting bhlh transcription factors that play a major role in controlling ja responses in vegetative tissue, but are not likely to play a role in reproductive tissue. we found that a closely related transcription factor, myc5 (bhlh28), w ...201525627909
heat shock factors in carrot: genome-wide identification, classification, and expression profiles response to abiotic stress.heat shock factors (hsfs) play key roles in the response to abiotic stress in eukaryotes. in this study, 35 dchsfs were identified from carrot (daucus carota l.) based on the carrot genome database. all 35 dchsfs were divided into three classes (a, b, and c) according to the structure and phylogenetic relationships of four different plants, namely, arabidopsis thaliana, vitis vinifera, brassica rapa, and oryza sativa. comparative analysis of algae, gymnosperms, and angiosperms indicated that the ...201525403331
pectin methylesterases: sequence-structural features and phylogenetic relationships.pectin methylesterases (pmes) are enzymes produced by bacteria, fungi and higher plants. they belong to the carbohydrate esterase family ce-8. this study deals with comparison of 127 amino acid sequences of this family containing the five characteristic sequence segments: 44_gxyxe, 113_qaval, 135_qdtl, 157_dfifg, 223_lgrpw (daucus carota numbering). six strictly conserved residues (gly44, gly154, asp157, gly161, arg225 and trp227) and six conservative ones (ile39, ser86, ser137, ile152, ile159 a ...200415337457
cytokinins: new apoptotic inducers in plants.high concentrations of cytokinins block cell proliferation and induce programmed cell death (pcd) in both carrot ( daucus carota l.) and arabidopsis thaliana (l.) heynh. cell cultures [13 and 27 micro m n(6)-benzylaminopurine (bap), respectively]. in the present work, cell death was scored by evan's blue staining and was also demonstrated to be programmed by various parameters, including chromatin condensation, oligonucleosomal dna degradation (laddering), and release of cytochrome c from mitoch ...200312520332
harlequin (hlq) and short blue root (sbr), two arabidopsis mutants that ectopically express an abscisic acid- and auxin-inducible transgenic carrot promoter and have pleiotropic effects on morphogenesis.plant growth and development is regulated by complex interactions among different hormonal, developmental and environmental signalling pathways. isolation of mutants in these processes is a powerful approach to dissect unknown mechanisms in regulatory networks. the plant hormones abscisic acid (aba) and auxin are involved in vegetative, developmental and environmental growth responses, including cell division and elongation, vascular tissue differentiation and stress adaptation. the uida (beta-g ...200212008902
primary phloem-specific expression of a zinnia elegans homeobox gene.some plant homeobox genes are expressed specifically in vascular cells and are assumed to function in the differentiation of specific types of vascular cells. however, homeobox genes exhibiting primary phloem-specific expression have not been reported. to elucidate the molecular mechanisms of vascular development, we undertook to isolate from zinnia elegans primary phloem-specific homeobox genes that may function in phloem development. an hd-zip type homeobox gene, zehb3, was isolated. this gene ...200111726705
isolation of the gene encoding carrot leafy cotyledon1 and expression analysis during somatic and zygotic embryogenesis.the arabidopsis thaliana lec1 gene regulates embryo morphology and seed maturation. for a better understanding of its function, we isolated a carrot (daucus carota l. cv. us-harumakigosun) counterpart of this gene, c-lec1, from a cdna library of carrot somatic embryos, since carrot is a better model plant for preparing large quantities of somatic embryos at the same developmental stage. the predicted amino acid sequence of c-lec1 is similar to that of lec1 and contains regions that are conserved ...200415051045
isolation and functional analysis of the 5'-flanking region of carrot top1beta gene coding for the beta isoform of dna topoisomerase i.we have isolated and functionally characterized the promoter region of the top1beta gene encoding carrot (daucus carota) dna topoisomerase ibeta. the major transcription start site was mapped by primer extension analysis 164 nt upstream the atg translation start codon. sequence analysis of the 5'-upstream region of the gene revealed the presence of a canonical tata-like box at position -35 bp and several cis-acting sequences, including a (ct)n element in the leader region of the gene, a myb-rela ...200312531479
carotene hydroxylase activity determines the levels of both α-carotene and total carotenoids in orange carrots.the typically intense carotenoid accumulation in cultivated orange-rooted carrots (daucus carota) is determined by a high protein abundance of the rate-limiting enzyme for carotenoid biosynthesis, phytoene synthase (psy), as compared with white-rooted cultivars. however, in contrast to other carotenoid accumulating systems, orange carrots are characterized by unusually high levels of α-carotene in addition to β-carotene. we found similarly increased α-carotene levels in leaves of orange carrots ...201424858934
study on folate binding domain of dihydrofolate reductase in different plant species and human beings.data base (ncbi and tigr) searches are made to retrieve protein sequences of different plant species namely medicago truncatula, pisum sativum, ricinus communis, arabidopsis thaliana, vitis vinifera, glycine max, daucus carota, oryza sativa japonica group, arabidopsis lyrata subsp. lyrata, brachypodium distachyon, oryza sativa indica group, zea mays and careful alignment of derived sequences shows 95% or higher identity. similarly, dhfr sequence of human being is also retrieved from ncbi. a phyl ...201424616563
nitrogen and carbohydrate storage in biennials originating from habitats of different resource availability.four biennial species (arctium tomentosum, cirsium vulgare, dipsacus sylvester and daucus carota) which originate from habitats of different nutrient availability were investigated in a 2-year experiment in a twofactorial structured block design varying light (natural daylight versus shading) and fertilizer addition. the experiment was designed to study storage as reserve formation (competing with growth) or as accumulation (see chapin et al. 1990). we show that (i) the previous definitions of s ...199328313438
tunicamycin-inhibited carrot somatic embryogenesis can be restored by secreted cationic peroxidase isoenzymes.somatic embryogenesis of carrot (daucus carota l.) is inhibited by the glycosylation inhibitor tunicamycin. this inhibition is reversible by the addition of correctly glycosylated glycoproteins which have been secreted into the culture medium. to identify the proteins responsible for complementation, glycoproteins present in the medium of embryo cultures were purified and tested for their activity in the tunicamycin inhibition/ complementation assay. a 38-kda glycoprotein was purified that could ...199124194238
homology of plant peroxidases: an immunochemical approach.antisera specific for the basic peroxidase from horseradish (amoracea rusticana) were used to examine homology among horseradish peroxidase isoenzymes and among basic peroxidases from root plants. the antisera cross-reacted with all tested isoperoxidases when measured by both agar diffusion and quantitative precipitin reactions. precipitin analyses provided quantitative measurements of homology among these plant peroxidases. the basic radish (raphanus sativus l. cv. cherry belle) peroxidase had ...198216662176
molecular cloning, sequence characterization of a novel pepper gene nadp-icdh and its effect on cytoplasmic male sterility.nadp-dependent isocitrate dehydrogenase (nadp-icdh) is an important enzyme involved in energy metabolism. the complete coding sequence of the pepper (capsicum annuum) nadp-icdh gene was amplified using a reverse transcriptase pcr based on the conserved sequence information of the tomato and other solanaceae plants and known highly homologous pepper ests. nucleotide sequence analysis revealed that the pepper nadp-icdh gene encodes a protein of 415 amino acids that has high homology with the prote ...201222653649
ambispora granatensis, a new arbuscular mycorrhizal fungus, associated with asparagus officinalis in andalucia (spain).a new dimorphic fungal species in the arbuscular mycorrhiza-forming glomeromycota, ambispora granatensis, was isolated from an agricultural site in the province of granada (andalucía, spain) growing in the rhizosphere of asparagus officinalis. it was propagated in pot cultures with trifolium pratense and sorghum vulgare. the fungus also colonized ri t-dna transformed daucus carota roots but did not form spores in these root organ cultures. the spores of the acaulosporoid morph are 90-150 μm diam ...201120952800
the ovary of the desert locust schistocerca gregaria contains a glycine- and proline-rich peptide that displays sequence similarities with a new class of gprp proteins from plants.a novel, highly hydrophobic, glycine- and proline-rich peptide was characterized in the ovary of the desert locust, schistocerca gregaria. the peptide was detected as one of the major peaks in a chromatographic separation of an acidic methanolic extract of 50 mature ovaries. electrospray mass spectrometry yielded a molecular mass of 6305 da. the partial amino acid sequence as determined by edman degradation based automated microsequencing is as follows: ala-tyr-pro-ala-his-gln-gly-tyr- pro-ala-h ...19989480819
a secreted peptide growth factor, phytosulfokine, acting as a stimulatory factor of carrot somatic embryo formation.somatic embryogenesis of the carrot (daucus carota l.) depends on a set of factors, some of which accumulate in culture medium (conditioned medium, cm). when embryogenic cell clusters were transferred to an embryo-inducing medium, addition of cm derived from somatic embryo culture markedly stimulated somatic embryo formation. the active principles were purified using a simple bioassay system and identified to be phytosulfokines (psks), sulfated oligopeptide growth factors originally isolated fro ...200010750705
high-throughput cryopreservation of plant cell cultures for functional genomics.suspension-cultured cell lines from plant species are useful for genetic engineering. however, maintenance of these lines is laborious, involves routine subculturing and hampers wider use of transgenic lines, especially when many lines are required for a high-throughput functional genomics application. cryopreservation of these lines may reduce the need for subculturing. here, we established a simple protocol for cryopreservation of cell lines from five commonly used plant species, arabidopsis t ...201222437846
factors contributing to the recalcitrance of herbaceous dicotyledons (forbs) to enzymatic deconstruction.many different feedstocks are under consideration for the practical production of biofuels from lignocellulosic materials. the best choice under any particular combination of economic, agronomic, and environmental conditions depends on multiple factors. the use of old fields, restored prairie, or marginal lands to grow biofuel feedstocks offers several potential benefits including minimal agronomic inputs, reduced competition with food production, and high biodiversity. however, a major componen ...201424708722
cloning of a cdna encoding dna topoisomerase i in daucus carota and expression analysis in relation to cell proliferation.dna topoisomerase i is an enzyme involved in several processes related to dna metabolism. despite the physiological importance, the regulation of top1 gene expression has not yet been investigated in plants. in order to monitor the possible correlation between levels of top1 transcripts and the proliferative state of the cell, two partially overlapping cdnas encoding dna topoisomerase i from daucus carota have been isolated from a poly(a)(+)-primed library, using an arabidopsis thaliana probe, a ...19968996105
assessment of allelopathic properties of aloe ferox mill. on turnip, beetroot and carrot.turnip (brassica rapa var. rapa l.), beetroot (beta vulgaris l.) and carrot (daucus carota l.) are common vegetables in south africa. the allelopathic potential of aqueous leaf and root extracts of aloe ferox mill.- a highly valued medicinal plant- was evaluated against seed germination and seedling growth of the three vegetables in petri dish experiments. the extracts were tested at concentrations of 2, 4, 6, 8, and 10 mg/ml. leaf extract concentrations above 4 mg/ml inhibited the germination o ...201223558992
nutritional content and antioxidant properties of pulp waste from daucus carota and beta vulgaris.this study reports the chemical composition and antioxidant potential of pulp waste from two vegetables, carrot (daucus carota) and beetroot (beta vulgaris). different in vitro assays used for determining antioxidant potential of extracts of pulp wastes were: 2,2-diphenyl-1-picrylhydrazyl (dpph) radical scavenging capacity, reducing power and total antioxidant activity by phosphomolybdenum method. total polyphenols, tannins and antioxidative components such as vitamin c, total carotenoids and β- ...201022691993
the genes abi1 and abi2 are involved in abscisic acid- and drought-inducible expression of the daucus carota l. dc3 promoter in guard cells of transgenic arabidopsis thaliana (l.) heynh.the aba insensitive1 (abi1) and abi2 genes encode homologous type-2c protein phosphatases with redundant yet distinct functions in abscisic acid (aba) responses. results from northern blot analysis showed that aba- and mannitol-inducible expression of the cor47 and cor78/lti78/rd29a (cor78) genes was more impaired in the abi2 mutant of arabidopsis thaliana (l.) heynh than in the abi1 mutant. furthermore, aba-plus-mannitol treatments were additive towards cor47 gene expression; however, the aba-d ...200010872217
heavy metals bioconcentration from soil to vegetables and assessment of health risk caused by their ingestion.the present study was undertaken to assess the non-carcinogenic human health risk of heavy metals through the ingestion of locally grown and commonly used vegetables viz. raphanus sativus (root vegetable), daucus carota (root vegetable), benincasa hispida (fruit vegetable) and brassica campestris leaves (leafy vegetable) in a semi-urbanized area of haryana state, india. heavy metal quantification of soil and vegetable samples was done using flame atomic absorption spectrophotometer. lead, cadmiu ...201424464601
translocation of nickel in xylem exudate of plants.topped plants of tomato (lycopersicon esculentum), cucumber (cucumis sativus), corn (zea mays), carrot (daucus carota), and peanut (arachis hypogaea) were treated with 0.5 to 50 micromolar ni (containing (63)ni) in nutrient solutions. xylem exudate was collected for 10 hours or, in the case of corn, for 20 hours at 5-hour intervals. electrophoresis of nutrient solution distributed all ni cathodically as inorganic ni(2+). low concentrations of ni in tomato exudate migrated anodically, presumably ...197116657780
determination of provitamin a of green leafy vegetables by high performance liquid chromatography and open column chromatography.the purpose of study was to determine the provitamin a value through beta-carotene analysis of five brazilian leafy vegetables: carrot leaves (daucus carota l.), beet leaves (beta vulgaris l.) "serralha" (sonchus oleracea l.), mint (mentha piperita ll.) and celery leaves (aplum graveolens. l.). two analysis methods were used: open-column chromatography (occ) and high performance liquid chromatography (hplc). two analysis methods were used: open-column chromatography (occ) and high performance li ...20139795484
structural and functional organisation of regenerated plant protoplasts exposed to microgravity on biokosmos 9.preparatory experiments for the iml-1 mission using plant protoplasts, were flown on a 14-day flight on biokosmos 9 in september 1989. thirty-six hours before launch of the biosatellite, protoplasts were isolated from hypocotyl cells of rapeseed (brassica napus) and suspension cultures of carrot (daucus carota). ultrastructural and fluorescence analysis of cell aggregates from these protoplasts, cultured under microgravity conditions, have been performed. in the flight samples as well as in the ...199211536948
a leucine-rich repeat protein of carrot that exhibits antifreeze activity.a gene encoding an antifreeze protein (afp) was isolated from carrot (daucus carota) using sequence information derived from the purified protein. the carrot afp is highly similar to the polygalacturonase inhibitor protein (pgip) family of apoplastic plant leucine-rich repeat (lrr) proteins. expression of the afp gene is rapidly induced by low temperatures. furthermore, expression of the afp gene in transgenic arabidopsis thaliana plants leads to an accumulation of antifreeze activity. our findi ...199910214940
the effect of exposure to microgravity on the development and structural organisation of plant protoplasts flown on biokosmos 9.preparatory experiments for the iml-1 (international microgravity laboratory) mission to be flown on the space shuttle in january, 1992, were performed on a 14 day flight on biokosmos 9 (kosmos 2044) in september 1989. the purpose of the experiment was to study the effect of weightlessness on protoplast regeneration. problems with late access to the space vehicle meant that the newly isolated protoplasts from hypocotyl cells of rapeseed (brassica napus l. cv niklas) and suspension cultures of ca ...199211541143
an improved uhplc-uv method for separation and quantification of carotenoids in vegetable crops.carotenoid identification and quantitation is critical for the development of improved nutrition plant varieties. industrial analysis of carotenoids is typically carried out on multiple crops with potentially thousands of samples per crop, placing critical needs on speed and broad utility of the analytical methods. current chromatographic methods for carotenoid analysis have had limited industrial application due to their low throughput, requiring up to 60 min for complete separation of all comp ...201425038701
screening for bioactive metabolites in plant extracts modulating glucose uptake and fat accumulation.dichloromethane and methanol extracts of seven different food and medicinal plants were tested in a screening platform for identification of extracts with potential bioactivity related to insulin-dependent glucose uptake and fat accumulation. the screening platform included a series of in vitro bioassays, peroxisome proliferator-activated receptor (ppar) γ-mediated transactivation, adipocyte differentiation of 3t3-l1 cell cultures, and glucose uptake in both 3t3-l1 adipocytes and primary porcine ...201425254050
bioaccessibility and arsenic speciation in carrots, beets and quinoa from a contaminated area of chile.consumption of vegetables grown in arsenic (as)-contaminated soils is an important exposure route to the element for humans. the present study is focused on locally-grown, frequently-consumed vegetables, such as carrots (daucus carota), beets (beta vulgaris) and quinoa (chenopodium) from the as-polluted chiu chiu area in northern chile. the latter region is affected both by as discharge from copper mining activity and natural as contamination, leading to a high as content in local food and water ...201627196992
particle surface characteristics may play an important role in phytotoxicity of alumina nanoparticles.the phytotoxicity of alumina nanoparticles loaded with and without phenanthrene (phen) was investigated by means of root elongation (re) experiments in this study. five plant species, zea mays (corn), cucumis sativus (cucumber), glycine max (soybean), brassica oleracea (cabbage), and daucus carota (carrot) were used in our study of phytotoxicity by root elongation experiments. the surface characteristics of phen-loaded and phen-nonloaded nanoparticles were investigated using the fourier transfor ...200516039401
influence of thermal processing on hydrolysis and stability of folate poly-gamma-glutamates in broccoli (brassica oleracea var. italica), carrot (daucus carota) and tomato (lycopersicon esculentum).the folate poly-gamma-glutamate profile, their concentrations, and hydrolysis by endogenous gamma-glutamyl hydrolase (ggh) were evaluated in broccoli, carrot and tomato. further studies on the effect of time and temperature on folate poly-gamma-glutamate hydrolysis and stability were carried out in broccoli since this vegetable showed the highest long-chain and total folate poly-gamma-glutamate concentration. the evolution of l-ascorbic acid, total phenols and trolox equivalent antioxidant capac ...201020329724
selecting and evaluating native plants for region-specific phytotoxicity testing.in this study, we evaluated methodology to determine risks to terrestrial native plant species from potential herbecide drift, focusing on 1) selection of native species for testing, 2) growth of these species, and 3) variability in herbicide response among native species and compared with crop plants. native plant species were selected for initial testing on the basis of spatial analysis, which indicated that species from illinois, usa, were at potential risk for off-target effects of herbicide ...200817973566
trypsin-induced atpase activity in potato mitochondria.potato mitochondria (solanum tuberosum var. russet burbank), which readily phosphorylate adp in oxidative phosphorylation, show low levels of atpase activity which is stimulated neither by mg(2+), 2,4-dinitrophenol, incubation with respiratory substrates, nor disruption by sonication or treatment with triton x-100, individually or in concert. treatment of disrupted potato mitochondria with trypsin stimulates mg(2+)-dependent, oligomycin-sensitive atpase activity 10- to 15-fold, suggesting the pr ...197616659532
nutrient status and phytotoxicity analysis of goat manure discharged from farms in south korea.the present study evaluated the phytotoxicity effect of goat manure (gm) collected from six different regions in south korea, namely, chupungnyeong (t1), hoengseong (t2), goesan (t3), sancheong (t4), jangsu (t5) and namwon (t6). phytotoxicity was assessed by means of the analysis of germination index (gi), relative seed germination (rsg), relative root elongation (rre) and vigor index (vi) using five commercial crop varieties, namely, sesame (sesamum indicum l.), chrysanthemum (chrysanthemum ind ...201727654710
metabolism of the herbicide glufosinate-ammonium in plant cell cultures of transgenic (rhizomania-resistant) and non-transgenic sugarbeet (beta vulgaris), carrot (daucus carota), purple foxglove (digitalis purpurea) and thorn apple (datura stramonium).the metabolism of the herbicide glufosinate-ammonium was investigated in heterotrophic cell suspension and callus cultures of transgenic (bar-gene) and non-transgenic sugarbeet (beta vulgaris). similar studies were performed with suspensions of carrot (daucus carota), purple foxglove (digitalis purpurea) and thorn apple (datura stramonium). 14c-labelled chemicals were the (racemic) glufosinate, l-glufosinate, and d-glufosinate, as well as the metabolites n-acetyl l-glufosinate and 3-(hydroxymeth ...200111455632
availability of arsenic, copper, lead, thallium, and zinc to various vegetables grown in slag-contaminated soils.to anticipate a possible hazard resulting from the plant uptake of metals from slag-contaminated soils, it is useful to study whether vegetables exist that are able to mobilize a given metal in the slag to a larger proportion than in an uncontaminated control soil. for this purpose, we studied the soil to plant transfer of arsenic, copper, lead, thallium, and zinc by the vegetables bean (phaseolus vulgaris l. 'dwarf bean modus'), kohlrabi (brassica oleracea var. gongylodes l.), mangold (beta vul ...200611401283
correlation between the suppression of glucose and phosphate uptake and the release of protein from viable carrot root cells treated with monovalent cations.treating carrot (daucus carota l.) discs with ice-cold nacl solutions for 30 minutes caused three effects that appear to be functionally related: the exchange of tissue ca(2+) and mg(2+) for na(+), the release of protein, and the suppression of active uptake of glucose and orthophosphate. cyclosis continued apparently unabated after treatment with nacl at concentrations of up to 0.25 m, so the cells remained viable and energetically competent. the correlation between the release of ca(2+) and mg ...197116657783
a parenchymatic medium solidifier for plant in vitro culture.a new material for the solidification of liquid culture media was prepared from plant parenchyma tissues by mechanical subdivision, solute extration and dessication from ethanol. it is suitable for in vitro culture and propagation of callus as well as shoot tip cultures. the following plant materials have been grown by means of the new medium solidifier: shoot cultures of betula pendula roth, gerbera jamesonii h. bolus ex hook and floribunda rose "triumph", callus tissues of daucus carota l. and ...198724248936
pectin esterification is spatially regulated both within cell walls and between developing tissues of root apices.monoclonal antibodies recognizing un-esterified (jim5) and methyl-esterified (jim7) epitopes of pectin have been used to locate these epitopes by indirect immunofluorescence and immunogold electron microscopy in the root apex of carrot (daucus carota l.). both antibodies labelled the walls of cells in all tissues of the developing root apex. immunogold labelling observed at the level of the electron microscope indicated differential location of the pectin epitopes within the cell walls. the un-e ...199024196931
composition of suberin-associated waxes from the subterranean storage organs of seven plants : parsnip, carrot, rutabaga, turnip, red beet, sweet potato and potato.the waxes associated with the suberin in the periderm of the underground storage organs of parsnip (pastinaca sativa l.), carrot (daucus carota l.), rutabaga (brassica napobrassica mill.), turnip (brassica rapa l.), red beet (beta vulgaris l.), sweet potato (ipomoea batatas l.) and potato (solanum tuberosum l.) were isolated, fractionated into hydrocarbon, wax ester, free fatty alcohol and free fatty acid fractions, and analyzed by combined gas chromatography and mass spectrometry. the amount of ...198024310190
binding of glycolytic enzymes to a particulate fraction in carrot and sugar beet storage roots : dependence on metabolic state.numerous studies have demonstrated a rapid increase in the respiration rate during aging of slices of tuber and storage roots. to determine the molecular mechanisms of this phenomenon, the role of enzyme binding to the subcellular particulate fraction has been assessed in carrot (daucus carota l.) and sugar beet (beta vulgaris l.). soluble versus particulate fractions were separated by centrifugation at 16,000g and both fractions assayed for the activities of six glycolytic enzymes. preparations ...198816665910
ionic thiocyanate (scn(-)) production, fate, and phytotoxicity in soil amended with brassicaceae seed meals.brassicaceae seed meals produce ionic thiocyanate (scn (-)), a bioherbicidal compound. this study determined the fate of scn (-) in a field soil amended with seed meals of sinapis alba, brassica juncea, and brassica napus and quantified crop phytotoxicity by monitoring carrot ( daucus carota) emergence. meals were applied at 1 or 2 t ha (-1), and soils were sampled to 35 cm for scn (-). maximum scn (-) (211 micromol kg (-1) of soil) was measured at 5 days in 0-5 cm samples from plots amended wit ...200818442242
mechanisms of the proliferation and differentiation of plant cells in cell culture systems.plant cell functions have been investigated in various cell culture systems. in this review, we summarize results obtained from investigations of gene expression during the cell cycle in synchronized cultures of catharanthus roseus during somatic embryogenesis in suspension cultures of daucus carota, during organogenesis in tissue cultures of arabidopsis thaliana and during the transdifferentiation of isolated mesophyll cells to tracheary elements in single-cell cultures of zinnia elegans.19947981037
effect of home freezing and italian style of cooking on antioxidant activity of edible vegetables.in this study, we analyzed the modifications of antioxidant activity consequent to 3 typical home cooking practices (steaming, boiling, and microwave cooking) in fresh and home frozen vegetables. six different vegetable species were examined: carrots (daucus carota l.), zucchini (cucurbita pepo l.), tomatoes (solanumn lycopersicum l.), green beans (phaseolus vulgaris l.), peas (pisum sativum l.), and yellow peppers (capsicum annuum l.). all vegetables were conventional products and were analyzed ...200819241586
a rapid method for purification of organelles for dna isolation: self-generated percoll gradients.self-generated percoll gradients have been used for rapid purification of crude chloroplasts and mitochondria, obtained by common differential centrifugation techniques. such purified organelles were used for isolating dna from safflower (carthamus tinctorius l.), carrot (daucus carota l.), various solanaceae, and numerous somatic hybrids. the method is simple, has the advantage of not requiring dnase, and is particularly well suited when only limited amounts of aseptically grown shoots are avai ...198824241541
influence of high-pressure processing on the profile of polyglutamyl 5-methyltetrahydrofolate in selected vegetables.in plants, folate occurs predominantly as 5-methyltetrahydrofolate (5mthf) polyglutamyl forms. differences in stability and bioavailability of food folate compared to synthetic folic acid have been attributed to the presence of the polyglutamyl chain. high-pressure processing (hpp) was tested for whether it might shorten polyglutamyl chains of 5mthf species in fresh vegetables by enabling action of native γ-glutamylhydrolase (ggh). a validated ultrahigh-performance reversed-phase liquid chromato ...201121770413
quantitative proteomics by 2de and maldi ms/ms uncover the effects of organic and conventional cropping methods on vegetable products.organic farming aims to be environmentally sound, but the question as to whether organic cropping brings more nutritional benefits to farmers and consumers than the conventional cropping needs still to be answered. to gain insights into the molecular effects of organic farming we used proteome analysis to analyze cabbage (brassica oleracea l. var 'capitata') and carrot (daucus carota var. 'sativus') our aim was to identify the metabolic pathways that are affected by different cropping regimes an ...201121757040
influence of plant growth on degradation of linear alkylbenzene sulfonate in sludge-amended soil.widespread application of sewage sludge to agricultural soils in denmark has led to concern about the possible accumulation and effects of linear alkylbenzene sulfonate (las) in the soil ecosystem. therefore, we have studied the uptake and degradation of las in greenhouse pot experiments. sewage sludge was incorporated into a sandy soil to give a range from very low to very high applications (0.4 to 90 mg dry wt. ha(-1)). in addition, las was added as water solutions. the soil was transferred to ...200611476504
barcoding melting curve analysis for rapid, sensitive, and discriminating authentication of saffron (crocus sativus l.) from its adulterants.saffron (crocus sativus l.) is one of the most important and expensive medicinal spice products in the world. because of its high market value and premium price, saffron is often adulterated through the incorporation of other materials, such as carthamus tinctorius l. and calendula officinalis l. flowers, hemerocallis l. petals, daucus carota l. fleshy root, curcuma longa l. rhizomes, zea may l., and nelumbo nucifera gaertn. stigmas. to develop a straightforward, nonsequencing method for rapid, ...201425548775
immobilization of plant protoplasts: viability studies.protoplasts of daucus carota ca68 and catharanthus roseus have been immobilized by entrapment in gelforming polysaccharides (kappa-carrageenan, agarose and alginate). uniform spherical beads of carrageenan and agarose containing the protoplasts have been prepared by utilizing an inert hydrophobic phase (vegetable oil). the entrapped protoplasts are viable and stabilized towards osmotic shock by the polymeric backbone. standard methods have been used to study the viability and integrity of the en ...198524253638
an eco-metabolomic study of host plant resistance to western flower thrips in cultivated, biofortified and wild carrots.domestication of plants and selection for agronomic traits may reduce plant secondary defence metabolites relative to their ancestors. carrot (daucus carota l.) is an economically important vegetable. recently, carrot was developed as a functional food with additional health-promoting functions. biofortified carrots contain increased concentrations of chlorogenic acid as an antioxidant. chlorogenic acid is involved in host plant resistance to western flower thrips (frankliniella occidentalis), o ...201323583013
volatiles identified from five stages of embryo development separated from a heterogeneous suspension culture of daucus carota.five stages of embryo development were fractionated from a mature culture of daucus carota (gelbe rheinsche), using a series of metal sieves. the composition of the population of embryos in each fraction was determined quantitatively from microscopic investigations. volatiles from samples of tissue from six stages of development were trapped on activated charcoal cartridges. these volatiles, some of which may play a significant role in the interaction of the plant with the carrot root fly (psila ...199124221846
radical scavenging and iron-chelating activities of some greens used as traditional dishes in mediterranean diet.this study aimed at evaluating the antioxidative activity of nine different families of greens. raphanus raphanistrum (wild radish), anchusa azurea (bugloss), daucus carota (wild carrot), sonchus oleraceus (sowthistle), papaver rhoeas (corn poppy), malva sylvestris (blue mallow), foeniculum vulgare (fennel), cichorium intybus (chicory) and salicornia europaea (jointed glasswort) are native to the mediterranean and are commonly consumed as a salad or an ingredient in some recipes. the antioxidati ...200414630594
simulation of germination of pioneer species along an experimental drought gradient.the germination of ten plant species from the iberian peninsula was assessed along a water deficit gradient between -0. 1652 (moist) and -0.4988 mpa (dry) of osmotic potential, created by addition of increasing concentrations of polyethylene glycol (peg 6000) to distilled water in which plants were grown hydroponically. the level and rate of germination of daucus carota and thapsia villosa significantly decreased with decreasing psi. seeds of dactylis glomerata and dittrichia viscosa had positiv ...200617405330
differential effect of sample preservation methods on plant and arbuscular mycorrhizal fungal dna.a wide range of methods are commonly used for preserving environmental samples prior to molecular analyses. however, the effect of these preservation methods on fungal dna is not understood. the objective of this study was to test the effect of eight different preservation methods on the quality and yield of dna extracted from bromus inermis and daucus carota roots colonized by the arbuscular mycorrhizal (am) fungus, glomus intraradices. the total dna concentration in sample extracts was quantif ...201020470836
a phosphorus-31 nuclear magnetic resonance study of phosphate uptake and storage in cultured catharanthus roseus and daucus carota plant cells.high resolution 31p nmr spectra (103.2 mhz) of oxygenated catharanthus roseus and daucus carota cells grown in suspension cultures were obtained using a solenoidal perfusion probe. the spectra showed resonances for various phosphorylated metabolites such as atp, adp, nad(p)(h), nucleoside diphosphoglucose, and sugar phosphates. the relative levels of the phosphorylated metabolites remained constant throughout the growth curve. no resonances for storage compounds such as polyphosphates, pyrophosp ...19853972837
apparent role of phosphatidylcholine in the metabolism of petroselinic acid in developing umbelliferae endosperm.studies were conducted to characterize the metabolism of the unusual fatty acid petroselinic acid (18:1cis[delta]6) in developing endosperm of the umbelliferae species coriander (coriandrum sativum l.) and carrot (daucus carota l.). analyses of fatty acid compositions of glycerolipids of these tissues revealed a dissimilar distribution of petroselinic acid in triacylglycerols (tag) and the major polar lipids phosphatidylcholine (pc) and phosphatidylethanolamine (pe). petroselinic acid comprised ...199412232131
plant cell growth under different levels of oxygen and carbon dioxide.an experimental system, in which gases of known composition were passed through flasks, was used to systematically study the effects of oxygen and carbon dioxide on plant cell growth. as expected, oxygen limiting conditions resulted in suppressed growth of catharanthus roseus cultures. oxygen limitations did not alter the amount of cell mass produced per gram of sugar consumed which suggests that the production of fermentative metabolites was limited. varying levels of carbon dioxide were observ ...199124226158
alkaline β-fructofuranosidases of tuberous roots: possible physiological function.alkaline invertase of roots of carrot (daucus carota l.) did not hydrolyze raffinose while the acid invertase from the same tissue showed with this sugar ca. 60% of the activity found with sucrose. the activity of the two invertases was inhibited by fructose to a different extent, the k i value being ca. 4×10(-2) m and 3×10(-1)m, respectively, for the alkaline and the acid invertases from the roots of both carrot and turnip (brassica rapa l.). it is proposed that fructose inhibition of acid inve ...197424442377
development of tuberous roots and sugar accumulation as related to invertase activity and mineral nutrition.sucrose storage in tuberous roots was not observed when the tissues had very high activities of acid invertase. high activities of the enzyme were always present in the roots at early stages of their development. in species where the activity of the enzyme decreased during root development, sucrose was stored. thus, acid invertase was undetectable in mature roots of carrots (daucus carota l.) where sucrose formed almost 80% of the dry matter. conversely, radish (raphanus sativus l.) and turnip ( ...197424442198
yield, chemical composition and nutritional quality responses of carrot, radish and turnip to elevated atmospheric carbon dioxide.future concentration of carbon dioxide in the atmosphere is very important due to its apparent economic and environmental impact in terms of climate change. however, a compressive assessment of its effect on the nutritional and chemical characteristics of food crops has yet to be established. in the present study the impact of elevated atmospheric co2 on the yield, chemical composition and nutritional quality of three root vegetables, carrot (daucus carota l. cv. t-1-111), radish (raphanus sativ ...201323576218
glutathione sulfotransferase inhibition activity of a self-fermented beverage, kanji.kanji, a liquid preparation of roots of daucus carota l. ssp. sativus (hoffm.) arcang. var. vavilovii mazk. (apiaceae), may inhibit glutathione sulfotransferase (gst) activity due to ferulic acid content.201727951746
heat shock disrupts cap and poly(a) tail function during translation and increases mrna stability of introduced reporter mrna.the effect of heat shock on translational efficiency and message stability of a reporter mrna was examined in carrot (daucus carota). heat shock of short duration resulted in an increase in protein yield, whereas repression was observed following extended exposure to the stress. regardless of the duration of the heat shock, a loss in the function of the 5[prime] cap [m7g(5[prime])ppp(5[prime])n, where n represents any nucleotide] and the 3[prime] poly(a) tail, two regulatory elements that work i ...199512228574
first report of leafminer lyriomyza spp. in daucus carota in brazil. 201627849253
promotion and computation of inhibitory effect on tyrosinase activity of herbal cream by incorporating indigenous medicinal plants.herbal cream imparts a chief role in regulating melanin production of skin. the phytoconstituents present in herbal cream impact biological functions of skin and contribute nutrients required for the healthy skin. in the present study, it was envisaged to prepare three batches of herbal cream (hc1, hc2 and hc3) containing ethanol extracts of emblica officinalis (fruits), daucus carota (root), mangifera indica (leaves), mentha arvensis (leaves), terminalia arjuna (bark) and cucumis sativus (fruit ...201424783796
carryover of perfluorooctanoic acid (pfoa) and perfluorooctane sulfonate (pfos) from soil to plant and distribution to the different plant compartments studied in cultures of carrots (daucus carota ssp. sativus), potatoes (solanum tuberosum), and cucumbers (cucumis sativus).a vegetation study was carried out to investigate the carryover of perfluorooctanoic acid (pfoa) and perfluorooctane sulfonate (pfos) from soil mixed with contaminated sewage sludge to potato, carrot, and cucumber plants. analysis was done by liquid-extraction using acetonitrile with dispersive spe cleanup and subsequent hplc-ms/ms. in order to assess the transfer potential from soil, transfer factors (tf) were calculated for the different plant compartments: tf = [pfc](plant (wet substance))/[p ...201121905714
[changes of glyco- and phospholipid contents during leaf senescence].the glyco- and phospholipid levels in green, green-yellow and yellow leaves of impatients balsamina l., daucus carota l. and cucurbita pepo l. were determined on a leaf area basis. in fagus silvatica l. leaves the kinetics of lipid breakdown during autumnal senescence was analysed. it was shown that yellow tissues still contain 40% to 50% of galactolipids and 50% to 60% of phospholipids present in green tissues. in the early stages of senescence of autumnal fagus leaves we found a more rapid dec ...197624430693
efficacy of eight larvicidal botanical extracts from khaya senegalensis and daucus carota against culex annulirostris.the failure to discover a significant new class of insecticides has led many researchers back to biodiscovery studies in the search for new and economically viable alternatives. after a preliminary screening of botanical extracts using descending series of concentrations (1,000, 500, 100, 50, and 5 mg/liter), 8 extracts from 2 potential botanical agents, khaya senegalensis (desrousseaux) and daucus carota l., were tested against 4th instars of culex annulirostris (skuse) following the standard w ...200617067042
a predictive nutritional model for plant cells and hairy roots.a structured nutritional model is proposed to describe growth and nutritional behavior of eschscholtzia californica suspension cells and catharanthus roseus and daucus carota hairy roots in in vitro culture. the model describes the cells specific growth rate from concentration of intracellular nutrients such as inorganic phosphate (pi), nitrogen sources (no(3) (-) and nh(4) (+)) and sugars. two-level michaelis-menten kinetics are used to describe pi and no(3) (-) uptake and simple michaelis-ment ...200817614323
seed germination in response to chemicals: effect of nitrogen and ph in the media.seed germination generally presents a peak in the next growing season after a fire. among other factors associated with fire are the increase of soil nitrogen and changes in the ph of the soil. in this study, we addressed the question, whether or not the germination response of eight species is linked with the increase in ph and nitrogenous compounds in the germination media? we assessed the separate and combined effects of nitrogenous compounds and ph on the percentage and rate of germination o ...200616850869
foliar-applied ethephon enhances the content of anthocyanin of black carrot roots (daucus carota ssp. sativus var. atrorubens alef.).black carrots (daucus carota ssp. sativus var. atrorubens alef.) constitute a valuable source of anthocyanins, which are used as natural red, blue and purple food colourants. anthocyanins and phenolic compounds are specialised metabolites, accumulation of which often requires elicitors, which act as molecular signals in plant stress responses. in the present study, ethephon, an ethylene-generating compound was explored as enhancer of anthocyanin and phenolic contents during growth of 'deep purpl ...201728376712
different lengths, copies and expression levels of the mitochondrial atp6 gene in male sterile and fertile lines of carrot (daucus carota l.).the male-sterile carrot is an effective material for carrot breeding. the atp6 gene is involved in carrot fertility. however, the differences in lengths, copies, and expression profiles of the atp6 gene in fertile and male-sterile lines of carrot are unclear. in this study, one copy atp6 gene was found in the mtdnas of 'kuroda' (fertility, 954 bp) and 'wuye-by' (male sterility, 819 bp) carrot lines, while two copies atp6 genes (wuye-l and wuye-d, 954 bp and 819 bp, respectively) were found in th ...201728335670
correlations between polyacetylene concentrations in carrot (daucus carota l.) and various soil parameters.this study assessed the concentrations of three falcarinol-type polyacetylenes (falcarinol, falcarindiol, falcarindiol-3-acetate) in carrots and the correlations between these and different soil traits. a total of 144 carrot samples, from three different harvests taken a single season, were analysed in terms of their polyacetylene concentrations and root development. on one of the harvesting occasions, 48 soil samples were also taken and analysed. the chemical composition of the soil was found t ...201628231155
characterization of a genomic region under selection in cultivated carrot (daucus carota subsp. sativus) reveals a candidate domestication gene.carrot is one of the most important vegetables worldwide, owing to its capability to develop fleshy, highly nutritious storage roots. it was domesticated ca. 1,100 years ago in central asia. no systematic knowledge about the molecular mechanisms involved in the domestication syndrome in carrot are available, however, the ability to form a storage root is undoubtedly the essential transition from the wild daucus carota to the cultivated carrot. here, we expand on the results of a previous study w ...201728149306
elevated carbon dioxide altered morphological and anatomical characteristics, ascorbic acid accumulation, and related gene expression during taproot development in carrots.the co2 concentration in the atmosphere has increased significantly in recent decades and is projected to rise in the future. the effects of elevated co2 concentrations on morphological and anatomical characteristics, and nutrient accumulation have been determined in several plant species. carrot is an important vegetable and the effects of elevated co2 on carrots remain unclear. to investigate the effects of elevated co2 on the growth of carrots, two carrot cultivars ('kurodagosun' and 'deep pu ...201628119712
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