Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| biochar reduces the bioaccumulation of pahs from soil to carrot (daucus carota l.) in the rhizosphere: a mechanism study. | the aim of this study was to reveal the mechanisms on how biochar reduces bioaccumulation of polycyclic aromatic hydrocarbons (pahs) in tuberous vegetables. corn straw-derived biochar pyrolyzed at 300°c (cb300) or bamboo-derived biochar pyrolyzed at 700°c (bb700) was amended into pah-contaminated soil planted with carrot (daucus carota l.). after 150days, 2% cb300 or 2% bb700 amendments significantly reduced the bioaccumulation of pahs in carrot root (p<0.05), especially for high-molecular-weigh ... | 0 | 28586746 |
| the sugars of the roots of daucus carota. | 1947 | 16654114 | |
| [the beta-carotene content of the carrot, daucus carota l]. | 1952 | 14948480 | |
| changes in the distribution of glucose-14c in alternative catabolic pathways induced by kinetin-analogue in the callus tissue of carrot (daucus carota l.). | 1962 | 14467516 | |
| [influence of daucus carota on the biogenesis of carotenoids in rhodotorula gracilis]. | 1963 | 14123053 | |
| characterization and assay of gazarin, bitter principle of fruits of daucus carota l. var. boissieri schweinf. | 1963 | 14032236 | |
| [chemical types of medicinal plants. 3. the different compositions of volatile oils of fruits from cultivated and wild carrots (daucus carota l. s. l.)]. | 1964 | 14344694 | |
| [studies on the bifidus factor. iii. studies on the various properties of the growth-promoting factor for bifidus bacilli contained in the carrot root (daucus carota)]. | 1964 | 14124663 | |
| studies on daucus carota, linn. i. pharmacological studies with the water-soluble fraction of the alcoholic extract of the seeds: a preliminary report. | 1966 | 5934261 | |
| studies on daucus carota linn. ii. cholinergic activity of the quaternary base isolated from water-soluble fraction of alcoholic extract of seeds. | 1966 | 5979224 | |
| [factors affecting embryogenesis in tissue cultures from domestic forms of umbellifera]. | the cultivation of tissues from domestic forms of umbellifera (daucus carota, apium-graveolens) on simple, synthetic media resulted in the production of adventive embryos if the salts and trace elements of white's medium were replaced by those of the formula of murashige and skoog. in contrast to results with embryos from different seeds the positive effect of these components on the production and the early development of the adventive embryos in the tissue cultures obviously was not dependent ... | 1966 | 24557788 |
| distribution of an indoleacetic acid-oxidase-inhibitor in the storage root of daucus carota. | indoleacetic acid (iaa)-oxidase from both secondary phloem and xylem was dependent on 2,4-dichlorophenol for activity, and was enhanced by addition of mn(2+). the ph optimum was 6.0 from both tissues. iaa-oxidase and its inhibitors were distributed differently in the secondary phloem and secondary xylem of carrot root. in the phloem a high iaa-oxidase activity was distributed uniformly along the radius but in the xylem a somewhat lower concentration decreased from the cambium. iaa-oxidase inhibi ... | 1967 | 16656541 |
| high phytochrome levels in cultured tissue of the wild carrot, daucus carota. | 1967 | 16656507 | |
| [the effect of ammonium ion on glucose metabolism in daucus carota cells]. | 1967 | 5618690 | |
| [formation of chloroplasts in tissue cultures of daucus carota in different hormonal conditions]. | 1967 | 5618688 | |
| investigations on the growth and metabolism of cultured explants of daucus carota : ii. effects of iron, molybdenum and manganese on metabolism. | knowing that the element fe essentially triggers the action of the coconut milk stimulus for the growth of carrot explants and thereafter interacts with added mo and mn, the effects of these trace elements on growth (fresh and dry weight) and metabolism of both nitrogenous and nitrogen-free metabolites have been determined. an outstanding effect of fe is to determine the level of protein synthesized, and the combination of mo with fe increases both protein and the content of non-protein nitrogen ... | 1968 | 24519729 |
| investigations on the growth and metabolism of cultured explants of daucus carota : i. effects of iron, molybdenum and manganese on growth. | the effects of fe, mo and mn on the growth of explants drawn from different carrot clones were investigated. the explants were cultured aseptically on purified basal media supplemented with coconut milk. the two components of growth, i.e. cell division and cell enlargement, seem to respond differentially to the trace elements in question. fe plays the key role and acts as a "trigger" of the action of coconut milk in stimulating cell division. neither mo nor mn could replace iron in this respect. ... | 1968 | 24519728 |
| transport of 3-o-methylglucose into and out of storage cells of daucus carota. | the movement of labeled 3-o-methylglucose (meg) into and out of thin carrot discs has been followed in order to gain information on sugar entry and exit mechanisms. little or no metabolism of this derivative appears to occur in the tissue, since no products were detected either by chromatography or by analysis of respiratory co(2).the curve relating entry to external concentration deviated somewhat from a rectangular hyperbola but suggested a carrier mechanism. glucose and meg each competitively ... | 1968 | 16656877 |
| [investigations on the relation between differentiation and the composition of soluble protein of tissue cultures and leaves of daucus carota]. | the correlations between differentiation, the amino acid composition of total protein and of soluble protein, and the disc electrophoretic distribution of soluble protein of carrot tissue cultures and of carrot plants (leaves) were studied. in spite of pronounced and characteristic changes in the electrophoretic distribution of the components of soluble protein in various developmental stages of both bioassays, no significant differences in the amino acid composition of protein were observed. wi ... | 1969 | 24504783 |
| [influence of nitrogen compounds and of auxin on embryogenesis in tissue cultures]. | investigations of a regularly reacting system for the analysis of embryo formation in tissue cultures of daucus carota led to new points of view about this process and to the clarification of controversial results. essential factors for the embryogenesis are the nitrogen and the auxin of the nutrient. inorganic (kno3, nh4no3) as well as organic (amino acids, amides) nitrogenous compounds enhance the embryo formation; glutamine and ammonium nitrate are especially efficient. auxin (2,4-d), on the ... | 1969 | 24504756 |
| synthesis and secretion of hydroxyproline containing macromolecules in carrots. i. kinetic analysis. | when disks of carrot (daucus carota) phloem parenchyma are incubated for 6 days there is a 10-fold increase in cell wall hydroxyproline due to the synthesis and secretion of hydroxyproline-containing macromolecules. the synthesis of these molecules and their secretion are demonstrated by measuring the kinetics of incorporation and of chase of (14)c-proline and hydroxyproline in different fractions of the cytoplasm and the cell wall. the hydroxyproline-containing molecules which are secreted are ... | 1969 | 16657187 |
| [demonstration of the nasturtium ringspot virus in the trumpet tree (catalpa bignonioides walt.) and carrots (daucus carota l.)]. | 1969 | 5396082 | |
| the contents of sterol derivatives (sterols + esters, glycosides and acylated glycosides) have been determined using thin layer chromatography in leaves and bulky and organs from carrots (daucus carota l.) potato (solanum tuberosum l.), onion (allium cepa l.) two varieties of radish (raphanus sativus l.) and gentiana purpurea l. plants. ether-soluble lipids of leaves contain 2.8-5.2%, those of storage tissue 3.1-8.9% sterol derivatives. the fractionation of lettuce (lactuca sativa l., burpee's r ... | 1970 | 11945480 | |
| phytochrome in cultured wild carrot tissue: ii. dark transformations. | disappearance and transformation of phytochrome in light-treated, dark-grown cultured tissue of wild carrot (daucus carota l.) have been analyzed. evidence is presented for the existence of two populations of far red-absorbing phytochromes: one which is subject to reversion, and a second which undergoes loss of photoreversibility. phytochrome levels and transformation characteristics have been compared for several strains and developmental stages of cultured wild carrot tissue. | 1970 | 16657464 |
| [characterization of root initiation and root senescence in callus- and organ-cultures of daucus carota through determination of the specific activity of the glutamate-dehydrogenase (nad)]. | in callus tissues of daucus carota rhizogenesis was influenced by addition to the cultures of solutions with different kinetin concentrations (0-0.1-0.5-2 mg/l) and a constant auxin content (1 mg/l). in these cultures the specific activity of glutamate-dehydrogenase (e.c. 1.4.1.2.), aspartate-aminotransferase (e.c. 2.6.1.1.), isocitrate-dehydrogenase (e.c. 1.1.1.42) and of acid phosphatase (e.c. 3.1.3.2.) was determined. before root initiation can be seen morphologically in tissues grown on low ... | 1970 | 24500017 |
| investigations on the growth and metabolism of cultured explants of daucus carota : iii. the range of responses induced in carrot explants by exogenous growth factors and by trace elements. | the responses of carrot explants to various growth-promoting agents and to certain trace elements with which they interact have been investigated. a great range in the metabolic behavior of the tissue may be brought about in this way. the responses to the exogenously applied substances are described in terms of the growth of the carrot explants in fresh weight and number of cells and also in terms of their metabolism, as shown by the final content and composition of the non-protein n compounds, ... | 1970 | 24500015 |
| kinetin-auxin ratio and development of chloroplasts in tissue cultures of daucus carota. | 1970 | 5444857 | |
| the greening of chromoplasts in daucus carota l. | evidence was obtained for the transformation of chromoplasts to chloroplasts in the cortex parenchyma of carrot during exposure to light. typical chromoplasts containing carotene crystals but no lamellar system were observed at the onset of illumination. the ensuing synthesis of chlorophyll and a lamellar system was accompanied by disappearance of the carotene crystals. only chloroplasts were present after 48 hr in the light. the different stages of plastid development were observed using the el ... | 1971 | 24493398 |
| investigations on the growth and metabolism of cultured explants of daucus carota : iv. effects of iron, molybdenum and the components of growth promoting systems and their interactions. | the induction and maintenance of growth in small, standard explants of carrot root exposed to a trace-element-free basal nutrient medium (b (**)) have been investigated. the organic growth factors that induce the growth are complex as represented by a trace-element-limited coconut-milk preparation (cm(**)) or the component parts of distinct growth promoting systems mediated by inositol or by 3-indoleacetic acid (iaa). in system i inositol interacts with growth factors from aesculus, of which a k ... | 1971 | 24487636 |
| [structure of the carotene crystals of daucus carota]. | 1971 | 5555933 | |
| translocation of nickel in xylem exudate of plants. | topped plants of tomato (lycopersicon esculentum), cucumber (cucumis sativus), corn (zea mays), carrot (daucus carota), and peanut (arachis hypogaea) were treated with 0.5 to 50 micromolar ni (containing (63)ni) in nutrient solutions. xylem exudate was collected for 10 hours or, in the case of corn, for 20 hours at 5-hour intervals. electrophoresis of nutrient solution distributed all ni cathodically as inorganic ni(2+). low concentrations of ni in tomato exudate migrated anodically, presumably ... | 1971 | 16657780 |
| correlation between the suppression of glucose and phosphate uptake and the release of protein from viable carrot root cells treated with monovalent cations. | treating carrot (daucus carota l.) discs with ice-cold nacl solutions for 30 minutes caused three effects that appear to be functionally related: the exchange of tissue ca(2+) and mg(2+) for na(+), the release of protein, and the suppression of active uptake of glucose and orthophosphate. cyclosis continued apparently unabated after treatment with nacl at concentrations of up to 0.25 m, so the cells remained viable and energetically competent. the correlation between the release of ca(2+) and mg ... | 1971 | 16657783 |
| tryptophan biosynthesis in nicotiana tabacum and daucus carota cell cultures: site of action of inhibitory tryptophan analogs. | 1972 | 4536831 | |
| effect of chromatographic fractions of daucus carota linn. (seeds) on fertility in female albino rats. | 1972 | 5078109 | |
| isolation and properties of deoxyribonucleic acid from protoplasts of cell suspension cultures of ammi visnaga and carrot (daucus carota). | a procedure is described for the isolation of native dna from protoplasts of ammi (ammi visnaga) and carrot (daucus carota) cells. protoplasts were produced from 40 grams of fresh cells by enzyme hydrolysis and lysed with sodium dodecyl sulfate. the dna was purified by treatment with pronase and ribonuclease. final isolation was achieved by sucrose density gradient centrifugation.the melting temperature of ammi and carrot dna in 0.15 m nacl and 15 mm trisodium citrate buffer, ph 7.0, was 84.0 c ... | 1972 | 16658166 |
| anthranilate synthetase from 5-methyltryptophan-susceptible and -resistant cultured daucus carota cells. | 1972 | 4652566 | |
| induced changes in permeability of plant cell membranes to water. | the half-time for tho equilibration was three times longer for a living carrot (daucus carota l.) cylinder than for a dead one. furthermore, the energy of activation of tho flux was more than twice as high for the living cylinder. passage through living membranes thus constitutes a rate-limiting step for tho flux in carrot tissue.co(2) increased the half-time (t((1/2))) for tho equilibration. treatment with abscisic acid or with tertiary butanol decreased t((1/2)). in neither case was the select ... | 1972 | 16658009 |
| investigations on the growth and metabolism of cultured explants of daucus carota : v. effects of trace elements and growth factors on the solutes accumulated. | earlier papers of this series relate to different growth-promoting substances and systems which, singly and in combination, have interacted with trace elements (mn and mo) and fe to induce growth and to affect the metabolism of aseptic cultures of carrot. the solutes of cultured carrot cells (k(+), na(+), cl(-), total solutes) are also affected. two clones were grown in 9 combinations of growth factors and under 4 trace-element regimes (a complete complement including fe, and this complement lac ... | 1973 | 24469575 |
| ethylene-induced phenylalanine ammonia-lyase activity in carrot roots. | ethylene enhanced the activity of phenylalanine ammonialyase in carrot (daucus carota l., var. "nauty") root tissue. slight increase in enzyme activity was exhibited by root discs incubated in ethylene-free air. it was probably due to the ethylene formed within the sliced tissue. addition of ethylene to the air stream increased phenylalanine ammonia-lyase activity and the total protein content of the discs until maximum activity was reached after 36 to 48 hours of incubation. the continuous pres ... | 1973 | 16658459 |
| further studies on the photosynthesis of carrot tissue cultures. | the influence of kinetin and sucrose on the photosynthetic activity of carrot (daucus carota) tissue cultures in relation to growth was investigated. the results showed that light contributes heavily to the growth of tissue cultures measured in terms of fresh and dry weight and cell division activity. in light, the fresh weight, dry weight, and number of cells per explant were about or more than doubled. this indicated that after the development of chloroplasts, carrot tissue cultures can grow a ... | 1973 | 16658393 |
| measurement of the five enzymes which convert chorismate to tryptophan in cultured daucus carota cell extracts. | 1973 | 4750744 | |
| monoamine oxidase inhibiting activity of daucus carota. | 1973 | 4783391 | |
| the effects of gelatin on aggregation of protoplasts from higher plants. | aggregation of protoplasts of allium fistulosum l., brassica chinensis l. and daucus carota l. was induced in great frequency with gelatin and early products of gelatin degradation. the optimal gelatin concentration was 2-5%. the aggregated protoplasts fused into groups and cell walls were formed around them in culture. | 1973 | 24458819 |
| anthiovulatory activity of five indigenous plants in rabbits. | the petroleum ether, alcoholic, and aqueous extracts of 5 indigenous plants, known to have antifertility activity in female rats and mice (areca catechu linn, carica papaya linn, daucus carota linn, mentha arvensis linn and polygonum hydropiper linn), were evaluated for their possible antiovulatory activity in rabbits with copper-induced ovulation. the alcoholic extract of mentha arvensis (leaves) and the petroleum ether extract of the roots of polygonum hydropiper were the most successful age ... | 1974 | 4442983 |
| cell wall and protoplast isoperoxidases in relation to injury, indoleacetic acid, and ethylene effects. | in tobacco (nicotiana tabacum) pith, sweet potato (ipomoea batatas), and carrot (daucus carota) storage roots, differences were found between cell wall and protoplast peroxidases in their isoenzyme patterns, activity, and reaction to tissue injury.in the pith of elongating tobacco internodes, 90% of total activity was associated with the walls, 80% of which was due to the ionically and covalently bound fractions. with senescence, the increase in activity occurred mainly in the protoplast and the ... | 1974 | 16658744 |
| alkaline β-fructofuranosidases of tuberous roots: possible physiological function. | alkaline invertase of roots of carrot (daucus carota l.) did not hydrolyze raffinose while the acid invertase from the same tissue showed with this sugar ca. 60% of the activity found with sucrose. the activity of the two invertases was inhibited by fructose to a different extent, the k i value being ca. 4×10(-2) m and 3×10(-1)m, respectively, for the alkaline and the acid invertases from the roots of both carrot and turnip (brassica rapa l.). it is proposed that fructose inhibition of acid inve ... | 1974 | 24442377 |
| development of tuberous roots and sugar accumulation as related to invertase activity and mineral nutrition. | sucrose storage in tuberous roots was not observed when the tissues had very high activities of acid invertase. high activities of the enzyme were always present in the roots at early stages of their development. in species where the activity of the enzyme decreased during root development, sucrose was stored. thus, acid invertase was undetectable in mature roots of carrots (daucus carota l.) where sucrose formed almost 80% of the dry matter. conversely, radish (raphanus sativus l.) and turnip ( ... | 1974 | 24442198 |
| the control of glycolysis in aged slices of carrot root tissue. | the possibility that the rate of glycolysis in aged slices of carrot (daucus carota l.) is controlled by the enzyme phosphofructokinase was examined, by changing the rate of metabolism, by supplying the tissue with potassium chloride, potassium phosphate and potassium citrate and measuring the subsequent changes in levels of metabolites. potassium chloride and potassium phosphate stimulate glycolysis, potassium citrate inhibits glycolysis and the associated changes in metabolites are consistent ... | 1974 | 24442325 |
| the pasteur effect in carrot root tissue. | the presence of a pasteur effect in aged discs of carrot (daucus carota l.) storage tissue and its apparent absence in fresh discs was confirmed. changes in the concentration of glycolytic intermediates have been determined, following the transfer of fresh and aged discs from air to nitrogen. in addition the changes in glycolytic intermediates associated with the ageing of the discs have also been measured. the results are analysed and interpreted to indicate that in aged discs the enzyme phosph ... | 1974 | 24442324 |
| [relationship between anthocyanin synthesis and activity of phenylalanine ammonia-lyase (pal) in callus cells of daucus carota]. | two celllines from daucus carota callus were tested for phenylalanine ammonia-lyase activity. the white callus (dcw) has only 15% of the enzyme activity of the blue callus (dcb). the enzyme activity reaches a maximum value after a cultivation period of 21 days, whereas the maximum in anthocyanin content is reached after 28 days. we can demonstrate here a good correlation between one of the enzymes and the end product of the anthocyanin biosynthesis.the crude extract from dcw cells has no inhibit ... | 1974 | 24458301 |
| fluxes of a nonelectrolyte and compartmentation in cells of carrot root tissue. | permeation of thiourea into cells of carrot (daucus carota l.) storage root discs was found to be governed by its concentration gradient only. the analysis of the course of thiourea efflux from preloaded discs shows two distinct exponential curves, apart from an initial very fast component. reasons are given for relating the first exponential part of the curve to efflux from the cytoplasm and the second exponential part to efflux from the vacuole.the rate constant for thiourea efflux through the ... | 1974 | 16658696 |
| transfer of algal chloroplasts into protoplasts of higher plants. | polyethylene-glycol treatment of mixed suspensions of isolated protoplasts and of chloroplasts induces chloroplast uptake by the protoplasts. chloroplasts of algal origin (vaucheria dichotoma (l.) ag.) were transferred with high frequency into protoplasts of daucus carota l. | 1974 | 24442619 |
| the duration of the cell cycle of daucus carota l. in vivo and in vitro. | 1975 | 1132430 | |
| involvement of the golgi apparatus in the synthesis and secretion of hydroxyproline-rich cell wall glycoproteins. | pulse labeling of carrot root phloem parenchyma (daucus carota l. cv. nantes) tissue with (14)c-proline followed by fractionation of the cytoplasmic organelles on sucrose gradients was used to determine the identity of the membranous organelles involved in the secretion of the hydroxyproline-rich glycoproteins of the cell wall. identification of the organelles was done through electron-microscopical observations and through the localization of marker enzymes on the sucrose gradients. enrichment ... | 1975 | 16659117 |
| release of hydrolytic enzymes from the cell walls of intact and disrupted carrot callus tissue. | the effect of ionic strength and ph on the solubility of acid invertase (e.c. 3.2.1.26.) and α-glucosidase (e.c. 3.2.1.20.) from carrot daucus carota l. tissue cultures is presented. the action of these two factors on disrupted tissue is contrasted with the effects of the same treatment on the solubility of these enzymes from intact tissue. the results are discussed in terms of a unified theory on the solubility behaviour of acid invertase and other hydrolytic enzymes in plant cells. | 1975 | 24430368 |
| [induction of anthocyanin biosynthesis in tissue cultures of daucus carota (author's transl)]. | 1975 | 1187875 | |
| determination of structure and composition of suberin from the roots of carrot, parsnip, rutabaga, turnip, red beet, and sweet potato by combined gas-liquid chromatography and mass spectrometry. | suberin from the roots of carrots (daucus carota), parsnip (pastinaca sativa), rutabaga (brassica napobrassica), turnip (brassica rapa), red beet (beta vulgaris), and sweet potato (ipomoea batatas) was isolated by a combination of chemical and enzymatic techniques. finely powdered suberin was depolymerized with 14% bf(3) in methanol, and soluble monomers (20-50% of suberin) were fractionated into phenolic (<10%) and aliphatic (13-35%) fractions. the aliphatic fractions consisted mainly of omega- ... | 1975 | 16659124 |
| characterization of carrot and tobacco cell cultures resistant to p-fluorophenylalanine. | this study describes the isolation and characterization of p-fluorophenylalanine-resistant diploid tobacco (nicotiana tabacum l.) and diploid carrot (daucus carota l.) cultured cell lines. the p-fluorophenylalanine-resistant tobacco and carrot lines can grow in medium containing p-fluorophenylalanine concentrations 10 to more than 100 times those which inhibit the growth of susceptible cells, respectively. the resistance trait was retained when the cells were grown in a medium lacking the phenyl ... | 1975 | 16659278 |
| nitrogen metabolism in plant cell suspension cultures: i. effect of amino acids on growth. | certain amino acids inhibit growth of tobacco (nicotiana tabacum l. var. xanthi), tomato (lycopersicon esculentum) carrot (daucus carota), and soybean (glycerine max l. co. mandarin) cell cultures when nitrate or urea are the nitrogen sources but not when ammonia is the nitrogen source. these amino acids also inhibit development of nitrate reductase activity (nadh:nitrate oxidoreductase ec 1.6.6.1) in tobacco and tomato cultures. threonine, the most inhibitory amino acid, also inhibits nitrate u ... | 1975 | 16659349 |
| pharmacological studies on daucus carota part i. | 1975 | 1178783 | |
| [metabolism of nicotinic acid in plant cell suspension cultures, iv: occurrence and metabolism of nicotinic acid n-alpha-arabinoside (author's transl)]. | application of nicotinic acid to cell suspension cultures of petroselinum hortense hoffm., daucus carota, nicotiana tabacum and nicotiana glauca leads to the formation of the recently isolated[2] nicotinic acid n-alpha-l-arabinoside. in these cell cultures the arabinoside is a metabolically active compound; the nicotinic acid moiety is used for nad synthesis and nicotinic acid degradation involving decarboxylation and ring fission. n-methylnicotinic acid (trigonelline) and nicotinic acid n-alpha ... | 1976 | 185135 |
| a method for eliminating the contribution of agrobacterium tumefaciens to studies of nucleic acid biosynthesis in crown gall tumors. | a procedure is described that permits the use of radiotracer methods for examining crown gall tumors in the presence of the initiating agent agrobacterium tumefaciens. on the basis of sensitivity testing using the disc diffusion technique and the minimum inhibitory concentration procedure and incorporation of radioactive phosphorus into nucleic acids, it was determined that a. tumefaciens was most sensitive to tetracycline, oxytetracycline, and methacycline. it was further demonstrated that nucl ... | 1976 | 16659494 |
| variation in germination and amino acid leakage of seeds with temperature related to membrane phase change. | leakages of amino acids and/or fluorescent material as functions of temperature between 15 and 40 c are reported for imbibed seeds of avena fatua l., lactuca sativa l., barbarea vulgaris r. br., amaranthus albus l., abutilon theophrasti medic., lychnis alba mill., daucus carota l., setaria faberi herrm., setaria viridis (l.) beauv., and datura stramonium l. the leakage indicates prominent increase in permeability of the plasmalemma in the 30 to 35 c range for 8 of the 10 kinds of seeds studied. ... | 1976 | 16659623 |
| trypsin-induced atpase activity in potato mitochondria. | potato mitochondria (solanum tuberosum var. russet burbank), which readily phosphorylate adp in oxidative phosphorylation, show low levels of atpase activity which is stimulated neither by mg(2+), 2,4-dinitrophenol, incubation with respiratory substrates, nor disruption by sonication or treatment with triton x-100, individually or in concert. treatment of disrupted potato mitochondria with trypsin stimulates mg(2+)-dependent, oligomycin-sensitive atpase activity 10- to 15-fold, suggesting the pr ... | 1976 | 16659532 |
| [changes of glyco- and phospholipid contents during leaf senescence]. | the glyco- and phospholipid levels in green, green-yellow and yellow leaves of impatients balsamina l., daucus carota l. and cucurbita pepo l. were determined on a leaf area basis. in fagus silvatica l. leaves the kinetics of lipid breakdown during autumnal senescence was analysed. it was shown that yellow tissues still contain 40% to 50% of galactolipids and 50% to 60% of phospholipids present in green tissues. in the early stages of senescence of autumnal fagus leaves we found a more rapid dec ... | 1976 | 24430693 |
| phenylalanine-ammonia-lyase activity and biosynthesis of anthocyanins and chlorogenic acid in tissue cultures of daucus carota (author's transl). | 1976 | 981382 | |
| estrogenic and pregnancy interceptory effects of carrot daucus carota seeds. | 1976 | 992821 | |
| on the mechanism of the uptake of vaucheria chloroplasts by carrot protoplasts treated with polyethylene glycol. | chloroplasts from the alga, vaucheria dichotoma (l.) ag., are taken up into protoplasts of carrot (daucus carota l.) during polyethylene-glycol treatment. since chloroplasts are found with equal frequency in uni- and multinucleate protoplasts, chloroplast uptake does not depend on protoplast fusion. however, higher frequencies of chloroplast uptake are observed when experimental conditions favor greater aggregation of protoplasts. the intracellular localization of chloroplasts is confirmed by el ... | 1976 | 24424824 |
| variation of cell cycle duration within suspension cultures of daucus carota and its consequence for the induction of ploidy changes with colchicine. | 1976 | 935509 | |
| growth and sucrose metabolism of carrot callus strains with normal and low acid invertase activity. | the properties of two strains of carrot (daucus carota) callus are presented. one has a very low acid invertase activity which is accompanied by differences in morphology and metabolic rate, but not in growth rate. we conclude that one of the main functions of plant acid invertases is in controlling the levels of sugars which, by interaction with hormones, affect differentiation, both morphological and biochemical. the effect of tris on sucrose metabolizing enzymes, and the cause of the "sucrose ... | 1976 | 28869296 |
| [flavonols and flavones of vegetables. viii. flavones of carrot leaves (author's transl)]. | the flavonoid constituents of carrot leaves (daucus carota l. ssp. sativa) were separated by means of cellulose column chromatography, and the following compounds were obtained crystalline and identified by usual procedures: luteolin 7-beta-d-glucoside as the main flavon, luteolin 4'-beta-d-glucoside, luteolin 7-beta-d-glucuronide, apigenin 7-beta-d-glucoside, apigenin 7-rutinoside, chrysoeriol 7-beta-d-glucoside. luteolin 7-rutinoside also was identified, but could not be obtained crystalline. | 1977 | 595898 |
| effects of ethephon, ethylene, and 2,4-dichlorophenoxyacetic acid on asexual embryogenesis in vitro. | asexual embryogenesis in daucus carota l. ;queen anne's lace' callus was suppressed by ethephon, ethylene, and 2,4-dichlorophenoxyacetic acid (2,4-d). the ethephon effect could be attributed to volatile and nonvolatile substances. the volatile component was probably entirely ethylene. ethylene was liberated in the cultures in direct proportion to ethephon added to the medium. autoclaving of ethephon caused a substantial decrease of measurable ethylene. continuous exposure of callus to 5 mul/l et ... | 1977 | 16660109 |
| somatic hybridisation of daucus carota and d. capillifolius by protoplast fusion. | protoplasts isolated from cultured cells of albino carrot (daucus carota) and normal green d. capillifolius were fused by polyethylene glycol. selection of somatic hybrid plants was based on the restoration of photosynthetic function in hybrids. green plantlets selected from embryo cultures were characterized on the basis of leaf morphology. the interspecific protoplast fusion resulted in green plants with leaves which were intermediate between those of the parents. the somatic hybrids between o ... | 1977 | 24317689 |
| morphometric measurements of daucus carota suspension culture cells. | standard stereologic methods have been applied to electron micrographs of daucus carota l. suspension culture cells. the relative frequencies of the different membrane systems within the cells have been determined and compared with published data form mature leaf cells. | 1977 | 24420523 |
| purification and substrate specifities of hydroxycinnamate: coa ligase from anthocyanin-containing and anthocyanin-free carrot cells. | callus cells of daucus carota l. have different phenylpropanoid pathways depending on the medium composition. cells propagated on a medium with gibberellic acid do not accumulate cyanidin but incorporate [(14)c]phenylalanine into chlorogenic acid at a high rate. cells grown on a medium free of gibberellic acid accumulate cyanidin in very large amounts. we here describe partial purification of hydroxycinnamate: coa ligase, and its properties in these two cell lines. the enzymes extracted from the ... | 1977 | 24420100 |
| synthesis of phenylalanine ammonia-lyase in anthocyanin-containing and anthocyanin-free callus cells of daucus carota l. | when callus cells of daucus carota are grown on a medium containing gibberellic acid (ga3) in a physiological concentration of 3x10(-6) m the cells cease to accumulate anthocyanins. this anthocyanin-free cell line has a very low activity of phenylalanine ammonia-lyase. after density labelling with d2o an intensive de novo synthesis of the phenylalanine ammonia-lyase (e.c. 4.3.1.5; pal) in the anthocyanin-containing cells does occur. 58% of the c-bound h-atoms are replaced by deuterium. the antho ... | 1977 | 24419894 |
| relation between auxin autotrophy and tryptophan accumulation in cultured plant cells. | auxin autotrophy was studied in cultured carrot (daucus carota l.), tobacco (nicotiana tabacum l.), and potato (solanum tuberosum l.) cell lines. of 10 carrot lines resistant to 5-methyltryptophan (5mt), which accumulate free tryptophan (trp) because of an altered control enzyme, 5 were auxinautotrophic while the normal parent line was not. carrot lines selected from the same parent line as resistant to other amino-acid analogs were not auxinautotrophic, like the parent. the only 5mt-resistant p ... | 1977 | 24419686 |
| influence of carbohydrates on quantitative aspects of growth and embryo formation in wild carrot suspension cultures. | wild carrot (daucus carota l.) cell suspensions were grown on a mineral salt medium supplemented with 10 mmmyoinositol in the presence and absence of 2.25 mum 2,4-dichlorophenoxyacetic acid (2,4-d), and a variety of carbon sources.the data obtained on growth and embryo number in the absence of 2,4-d show that wild carrot suspensions were able to utilize sucrose, glucose, fructose, galactose, mannose, maltose, raffinose, or stachyose as a carbon source. a highly significant correlation between dr ... | 1977 | 16659793 |
| rapidly-labelled rna species isolated from cell suspensions of daucus carota. | 1977 | 918306 | |
| correlation between phenylalanine ammonia lyase activity and phenolic biosynthesis in p-fluorophenylalanine-sensitive and -resistant tobacco and carrot tissue cultures. | phenylalanine ammonia lyase (pal) activity was measured in p-fluorophenylalanine (pfp)-sensitive and -resistant tobacco (nicotiana tabacum l.) and carrot (daucus carota l.) cell lines which are known to oversynthesize phenylalanine. a correlation between phenolic levels and pal activities was detected. the phenylalanine analog-resistant and -sensitive carrot cells showed no differences in the accumulation of phenolic compounds and pal activities. the pfp-resistant tobacco cells, however, had 10 ... | 1977 | 16659891 |
| effects of ophiobolin a on ion leakage and hexose uptake by maize roots. | ophiobolin a, a sesterterpene metabolite of helminthosporium maydis, nisikado and miyake, stimulates net leakage of electrolytes and glucose from maize (zea mays l.) seedling roots. treatment of the roots with ophiobolin a at a concentration of 10 mug/ml (25 mum) inhibits uptake of 10 mm 2-deoxyglucose by 50% and of 0.5 mm 2-deoxyglucose by 85%. compartmental analysis of the efflux of 3-o-methylglucose failed to show a similar effect of ophiobolin a on the rate of efflux of hexose. the inhibitio ... | 1977 | 16659965 |
| effect of glyphosate on carrot and tobacco cells. | the growth of suspension-cultured carrot (daucus carota l.) and tobacco (nicotiana tabacum l. cv. xanthi) cells was inhibited by glyphosate (n-[phosphonomethyl]glycine). this inhibition was reversed by adding combinations of phenylalanine, tyrosine, and tryptophan or casein hydrolysate. casein hydrolysate and phenylalanine + tyrosine + tryptophan were the most effective treatments. reversal of glyphosate-induced inhibition occurred only if the aromatic amino acids were added during the first 8 d ... | 1977 | 16660038 |
| repression of asexual embryogenesis in vitro by some plant growth regulators. | a factor that represses asexual embryogenesis has been observed in the rutaceae, with particularly high concentrations in the naturally monoembryonic cultivars. this investigation was an initial step towards identifying the factor. citrus reticulata blanco ponkan mandarin nucellus explants and daucus carota l. 'queen anne's lace' callus were employed to examine effects of known plant growth regulators and to determine possible identity of one or more of them with the repressive factor. the chala ... | 1977 | 145403 |
| bacitracin inhibits the synthesis of lipid-linked saccharides and glycoproteins in plants. | the particulate enzyme fraction from mung bean (phaseolus aureus) seedlings catalyzes the incorporation of mannose from gdp-[(14)c]mannose into mannosyl-phosphoryl-dolichol and of n-acetylglucosamine from udp-[(3)h]n-acetylglucosamine into n-acetylglucosamine-pyrophosphoryl-polyisoprenol. bacitracin inhibits the transfer of both of these sugars into the lipid-linked saccharides with 50% inhibition being observed at 5 mm bacitracin. this antibiotic did not inhibit the transfer of glucose from udp ... | 1978 | 16660520 |
| ontogeny of daucus carota infected with meloidogyne hapla. | the ontogeny of carrots (daucus carota cv. 'spartan premium') grown under greenhouse conditions in pots of organic soil infected with meloidogyne hapla was influenced detrimentally as early as 4 days after seeding, as determined through analysis of plant surface area, dry weight, fresh weight, net assimilation rate, relative growth rate, and leaf-area ratio. only 58% of the diseased carrots were suitable for fresh market, compared with 97% of those grown in nematode-free soil. growth and develop ... | 1978 | 19305837 |
| regulatory isoenzymes of aspartate kinase and the control of lysine and threonine biosynthesis in carrot cell suspension culture. | aspartate kinase (ec 2.7.2.4) from carrot (daucus carota l.) cell suspension culture has been partially resolved into lysine-sensitive and threonine-sensitive components by gel filtration chromatography. the yield of lysine-sensitive aspartate kinase changed independently of the yield of the threonine-sensitive activity during the 4-week growth cycle of the culture, and this provides additional evidence for the existence of two independently regulated isoenzymes. exogenously supplied lysine and ... | 1978 | 16660554 |
| polyamine metabolism in embryogenic cells of daucus carota: i. changes in intracellular content and rates of synthesis. | changes in the metabolism of polyamines, which seem to be involved in transcription and translation in animal systems, have been studied in cultured cells of daucus carota (carrot) undergoing embryogenesis. putrescine levels were elevated by as much as 2-fold over the control within 24 hours after transfer of the cells to embryogenic medium. spermidine levels were elevated also but spermine levels appeared to be lower in embryogenic cells. embryogenic cells incorporated [(14)c]arginine into putr ... | 1978 | 16660531 |
| inhibition of deoxyribonuclease activity in the medium surrounding plant protoplasts. | after 1 hour, exogenous deoxyribonucleic acid was degraded within a culture medium at 25 c (ph 6) containing protoplasts of daucus carota l. var. sativa. low temperature incubation (1 c) or the addition of 45 millimolar sodium citrate to the medium eliminated dnase activity for at least 4.5 hours. this dnase activity was not reduced at ph 7 or 9, nor by addition of 200 millimolar adenosine 5'-triphosphate.techniques were developed to ensure high protoplast plating efficiencies and high regenerat ... | 1978 | 16660499 |
| morphogenetic responses of cultured totipotent cells of carrot (daucus carota var. carota) at zero gravity. | an experiment designed to test whether embryos capable of developing from isolated somatic carrot cells could do so under conditions of weightlessness in space was performed aboard the unmanned soviet biosatellite kosmos 782 under the auspices of the joint united states-soviet biological satellite mission. space flight and weightlessness seem to have had no adverse effects on the induction of embryoids or on the development of their organs. a portion of the crop of carrot plantlets originated in ... | 1978 | 17847331 |
| electron microscopic studies in cultivated plants. ii. fresh and stored roots of daucus carota l. | scanning (sem) and transmission (tem) electron microscopic studies in fresh and stored roots (4 months at an average temperature of +8 degrees c) of daucus carota l. (carrots) were carried out. chief stress was laid on the analysis of the histological and cytological structure of the secondary phloem parenchyma cell. sem images show the three-dimensional histological arrangement of the cells. moreover, the outlines of lipid droplets, mitochondria, starch grains, and carotin pigment crystals are ... | 1978 | 636662 |
| dna, rna and protein content of tissue during growth and embryogenesis in wild-carrot suspension cultures. | a highly selected population of cells (clumps from 63 to 125 micron in diameter), obtained by screening 14-day-old stock suspension cultures of wild carrot (daucus carota l.), was used to initiate cultures in this study. time-course changes in dna, rna and protein were followed when these cultures were grown in the presence or absence of 2.25 mum 2,4-dichlorophenoxyacetic acid (2,4-d). the data show that growth of these cultures, particularly in the early part of the growth curve, is different f ... | 1978 | 669724 |
| growth and embryo formation in wild-carrot suspension cultures with ammonium ion as a sole nitrogen source. | wild-carrot (daucus carota l.) suspension cultures grew and produced embryos on ammonium ion as a sole source of nitrogen in the absence of any exogenous kreb's cycle acid when the ph of the medium was controlled by continuous titration with with koh or khco3. | 1978 | 27444 |
| regulation of lysine and threonine synthesis in carrot cell suspension cultures and whole carrot roots. | aspartokinase (ec 2.7.2.4), homoserine-dehydrogenase (ec 1.1.1.3) and dihydrodipicolinic-acid-synthase (ec 4.2.1.52) activities were examined in extracts from 1-year-old and 11-year-old cell suspension cultures and whole roots of garden carrot (daucus carota l.). aspartokinase activity from suspension cultures was inhibited 85% by 10 mm l-lysine and 15% by 10mm l-threonine. in contrast, aspartokinase activity from whole roots was inhibited 45% by 10 mm lysine and 55% by 10 mm threonine. this dif ... | 1978 | 24414879 |
| oleosomes (spherosomes) from daucus carota suspension culture cells. | isolated oleosomes from daucus carota l. cells are lipid droplets consisting mainly of triacylglycerols (>97%) and very little protein (1-2%). the boundary between the lipid phase and the cytosol, which is visible on electron micrographs, is not built up by a true phospholipid-containing unit or half unit membrane. enzymatic activities of lipid metabolism were not found to be associated with oleosomes with the exception of very low (contaminating) acyl-coa:1,2-diacylglycerol acyltransferase (ec ... | 1978 | 24414559 |
| lipids, lipid turnover, and phospholipase d in plant suspension culture cells (daucus carota). | the lipid pattern of daucus carota l. suspension culture cells and other plant cell strains was analyzed. phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, and triacylglycerol were the main components. the characteristic plastidal and mitochondrial lipids could also be identified. all strains tested exhibited a phospholipase d activity. several lipid precursors were found to be well utilized by the cells and to be special markers for certain lipids or parts of the lipid molecu ... | 1978 | 24414107 |
| isolation and identification of substances inducing formation of tracheary elements in cultured carrot-root slices. | the roots of a carrot (daucus carota l.) cultivar, kuroda-gosun, contain active substances causing formation of tracheary elements in cultured root phloem slices. in the roots of another carrot cultivar, hokkaidô-gosun, such substances could not be detected but when root-phloem slices of this cultivar were cultured in the light, active substances were produced in the tissue. from the behavior in thinlayer chromatography and data from gas chromatography and mass spectrometry, the major active sub ... | 1978 | 24413942 |
| the influence of gibberellic acid on reassociation kinetics of dna of daucus carota l. | carrot dna, extracted from the tap root of untreated and gibberellic acid (ga3)-treated plants and of different varieties, was analyzed by reassociation kinetics. differences due to ga3 treatment appear mainly in the intermediate repeated dna region. differences in approximately the same region are found using carrot dna of different varieties, which also show differences in the slow reassociating sequences. by hybridizing a "family" of the "unique" dna range with dna obtained from ga3-treated p ... | 1978 | 24408253 |
| change in the proportion of two aspartokinases in carrot root tissue in response to in vitro culture. | two isofunctional aspartokinases (ec 2.7.2.4) exist in fresh root tissue of carrot (daucus carota, cv. oogata sanzun). the threoninesensitive portion constitutes about 70% of the activity; the lysinesensitive, less than 20%. culture of slices of carrot tissue for 3 days reversed the ratio as the lysine-sensitive activity preferentially increased. inhibition by threonine and lysine was additive in both enzyme preparations from fresh and cultured tissues. the activities were resolved into two dist ... | 1978 | 16660222 |
| quantitative studies of embryogenesis in normal and 5-methyltryptophan-resistant cell lines of wild carrot : the effects of growth regulators. | the frequency of embryo formation was determined in normal and 5-methyltryptophan-resistant (5-mt(r)) cell lines of wild carrot (daucus carota l.) grown in the presence or absence of 2-isopentenyladenine (2-ip) and 2,4-dichlorophenoxyacetic acid (2,4-d). 2-ip stimulated the intitation of embryo formation and also accelerated embryo development. 2.4-d inhibited embryo differentiation at several stages: at 0.1 mg/l, it stopped regeneration at the earliest stage, resulting in callus growth instead ... | 1979 | 24311038 |
| expression of aspartokinase, dihydrodipicolinic acid synthase and homoserine dehydrogenase during growth of carrot cell suspension cultures on lysine- and threonine-supplemented media. | reduction in the amounts of activity of the first enzyme, aspartokinase (ec 2.7.2.4) and two branch-point enzymes, dihydrodipicolinic acid synthase (ec 4.2.1.52) and homoserine dehydrogenase (ec 1.1.1.3), located in the pathway for the synthesis of aspartate-family amino acids, occurred when cell suspension cultures of daucus carota l. var. danvers were grown in media containing 2 mm threonine or 2 mm lysine, endproducts of the pathway. activity of the lysine-sensitive form of aspartokinase was ... | 1979 | 232596 |