Publications

in rhizobium meliloti, the operon associated with the nod box n5 comprises nodl, noea and noeb, three host-range genes specifically required for the nodulation of particular medicago species.	in rhizobium meliloti, the genes required for nodulation of legume hosts are under the control of dna regulatory sequences called nod boxes. in this paper, we have characterized three host-specific nodulation genes, which form a flavonoid-inducible operon down-stream of the nod box n5. the first gene of this operon is identical to the nodl gene identified by baev and kondorosi (1992) in r. meliloti strain ak631. the product of the second gene, noea, presents some homology with a methyltransferas ...	1995	8801423
enzymatic radiolabelling to a high specific activity of legume lipo-oligosaccharidic nodulation factors from rhizobium meliloti.	in this paper we describe the two-step coupled 35s-radiolabelling of the lipo-oligosaccharidic nodulation (nod) factors of the bacterium rhizobium meliloti to a specific radioactivity of 800 ci/mmol. these radiolabelled nod factors bind to a particulate fraction from roots of the bacterium's symbiotic host, medicago truncatula, with an equilibrium dissociation constant (kd) of 117 nm, similar to that observed with a synthetic tritiated ligand. the first step of the 35s-labelling involves the syn ...	1995	7864819
te7, an inefficient symbiotic mutant of medicago truncatula gaertn. cv jemalong.	a mutagenesis program using ethylmethane sulfonate on medicago truncatula gaertn cv jemalong, an annual, autogamous and diploid lucerne, permitted the isolation of a mutant (te7) unable to establish an effective nitrogen-fixing symbiosis, [nod+fix-], with rhizobium meliloti wild-type strains. the mutant phenotype is characterized by an altered infection process that leads to the formation of two kinds of inefficient nodules on the same root system. a certain proportion of the nodules are small, ...	1995	12228341
enod40, a gene expressed during nodule organogenesis, codes for a non-translatable rna involved in plant growth.	rhizobium meliloti can interact symbiotically with medicago plants, thereby inducing root nodules. however, certain medicago plants can form nodules spontaneously, in the absence of rhizobia. a differential screening was performed using spontaneous nodule versus root cdnas from medicago sativa ssp. varia. transcripts of a differentially expressed clone, msenod40, were detected in all differentiating cells of nodule primordia and spontaneous nodules, but were absent in fully differentiated cells. ...	1994	7957074
a new proline-rich early nodulin from medicago truncatula is highly expressed in nodule meristematic cells.	we cloned and characterized mtprp4, a new member of the repetitive proline-rich protein gene family in medicago truncatula. the sequence of mtprp4 predicts a 62-kd protein consisting of a 22-amino acid n-terminal signal peptide and a 527-amino acid repetitive proline-rich domain composed of three repetitive pentapeptide motifs arranged into two decapeptide repeats: ppvekppvhk and ppvekppvyk. mtprp4 is the largest prp described to date and contains repeated motifs that have not previously been fo ...	1994	7919991
rhizobium meliloti elicits transient expression of the early nodulin gene enod12 in the differentiating root epidermis of transgenic alfalfa.	to study the molecular responses of the host legume during early stages of the symbiotic interaction with rhizobium, we have cloned and characterized the infection-related early nodulin gene mtenod12 from medicago truncatula. in situ hybridization experiments have shown that, within the indeterminate medicago nodule, transcription of the mtenod12 gene begins in cell layers of meristematic origin that lie ahead of the infection zone, suggesting that these cells are undergoing preparation for bact ...	1992	1446169
lectin genes from the legume medicago truncatula.	we report the cloning and characterization of two lectin genes from medicago truncatula, designated mtlec1 and mtlec2. the two genes show a high degree of homology and apparently belong to a small multigene family. mtlec1 appears to encode a functional lectin with 277 amino acids, whereas mtlec2 is probably non-functional, since a frameshift mutation (insertion of two nucleotides) leads to premature translation termination after only 98 amino acids. the deduced amino acid sequence of the polypep ...	1992	1511126
genetic transformation of medicago truncatula using agrobacterium with genetically modified ri and disarmed ti plasmids.	fertile transgenic plants of the annual pasture legume medicago truncatula were obtained by agrobacterium-mediated transformation, utilising a disarmed ti plasmid and a binary vector containing the kanamycin resistance gene under the control of the cauliflower mosaic virus 35s promoter. factors contributing to the result included an improved plant regeneration protocol and the use of explants from a plant identified as possessing high regeneration capability from tissue culture. genes present on ...	1992	24213541
synchronous expression of leghaemoglobin genes in medicago truncatula during nitrogen-fixing root nodule development and response to exogenously supplied nitrate.	two leghaemoglobin genes from the diploid, autogamous medicago truncatula (mtlb1 and mtlb2) have been cloned and their nucleotide sequences determined. the deduced amino acid sequences encoded by these two genes differ significantly (18%), confirming that they belong to different sub-groups of medicago leghaemoglobin genes. rnase protection experiments have been used to show that both genes are transcriptionally active, and are expressed specifically in the nitrogen-fixing root nodule of m. trun ...	1991	1883994
the effect of zeranol and testosterone on merino wethers exposed to highly oestrogenic subterranean clover pasture.	groups of merino wethers treated with 2 doses of zeranol (6 mg and 12 mg), or testosterone cyclopentyl propionate (150 mg) and untreated controls were grazed at 2 sites, one an oestrogenic subterranean clover (trifolium subterraneum) pasture and the other a low oestrogen medic (medicago truncatula) pasture. the influence of oestrogenic subterranean clover on these treatments was assessed by measuring changes in teat length, bulbourethral gland weight and pathology, bodyweight, carcase weight, dr ...	1986	3767798
competitive abilities of rhizobium meliloti strains considered to have potential as inoculants.	twenty four strains of rhizobium meliloti considered to have potential for inoculant production were grouped in pairs and tested for their ability to compete for nodulation on medicago sativa, medicago truncatula, and medicago littoralis. at the outset, each pair of strains, which consisted of a wild type and a selected streptomycin-resistant mutant of another strain, was tested in an autoclaved soil. six strain pairs, each consisting of a good and a poor competitor, reacted consistently when te ...	1982	16346072