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plant growth promotion abilities and microscale bacterial dynamics in the rhizosphere of lupin analysed by phytate utilization ability.in the rhizosphere, phosphorus (p) levels are low because of p uptake into the roots. rhizobacteria live on carbon (c) exuded from roots, and may contribute to plant nutrition by liberating p from organic compounds such as phytates. we isolated over 300 phytate (na-inositol hexa-phosphate; na-ihp)-utilizing bacterial strains from the rhizosheath and the rhizoplane of lupinus albus (l.). almost all of the isolates were classified as burkholderia based on 16s rdna sequence analysis. rhizosheath is ...200515683400
alterations induced by glyphosate on lupin photosynthetic apparatus and nodule ultrastructure and some oxygen diffusion related proteins.the effects of glyphosate on protein metabolism, mesophyll cell ultrastructure and nodule ultrastructure and functioning of lupinus albus cv. multolupa inoculated with bradyrhizobium sp. (lupinus) were investigated. young leaves and nodules were especially affected because these organs act as sinks of the herbicide. the alterations on nodular and chloroplast ultrastructure varied depending on herbicide concentration and time of exposure. after 3 days of 2.5 mm glyphosate application some toxic e ...200516324849
transgenic proteoid roots of white lupin: a vehicle for characterizing and silencing root genes involved in adaptation to p stress.white lupin (lupinus albus l.) has become an illuminating model for the study of plant adaptation to phosphorus (p) deficiency. it adapts to -p stress with a highly coordinated modification of root development and biochemistry resulting in short, densely clustered secondary roots called proteoid (or cluster) roots. in order to characterize genes involved in proteoid root formation and function in a homologous system, we have developed an agrobacterium rhizogenes-based transformation system for w ...200516297074
phyllobacterium trifolii sp. nov., nodulating trifolium and lupinus in spanish soils.bacterial strain petp02(t) was isolated from nodules of trifolium pratense growing in a spanish soil. phylogenetic analysis of the 16s rrna gene sequence showed that this strain represents a member of the genus phyllobacterium. however, divergence found with the 16s rrna gene sequence of the single recognized species of this genus, phyllobacterium myrsinacearum, indicated that strain petp02(t) belongs to a different species. the results of dna-dna hybridization, phenotypic tests and fatty acid a ...200516166699
raffinose family of oligosaccharides from lupin seeds as prebiotics: application in dairy products.the raffinose family of oligosaccharides (rfos) isolated from lupin seeds (lupinus albus var. multolupa) was evaluated for bifidogenic effects during the manufacture of probiotic fermented milk. a mixed starter inoculum was composed of bifidobacterium lactis bb-12 and lactobacillus acidophilus (1:1). lupins are a rich source of rfos that can be used as functional food ingredients. the addition of rfos to milk increased b. lactis bb-12 and l. acidophilus populations at the final fermentation time ...200515954717
soil to plant transfer of 238u, 226ra and 232th on a uranium mining-impacted soil from southeastern china.both soil and plant samples of nine different plant species grown in soils from southeastern china contaminated with uranium mine tailings were analyzed for the plant uptake and translocation of 238u, 226ra and 232th. substantial differences were observed in the soil-plant transfer factor (tf) among these radionuclides and plant species. lupine (lupinus albus) exhibited the highest uptake of 238u (tf value of 3.7x10(-2)), while chinese mustard (brassica chinensis) had the least (0.5x10(-2)). how ...200515878419
diversity of transcripts of nitrite reductase genes (nirk and nirs) in rhizospheres of grain legumes.transcription of the nirk and nirs genes coding for dissimilatory bacterial nitrite reductases was analyzed by reverse transcription pcr (rt-pcr) of mrna isolated from rhizosphere samples of three economically important grain legumes at maturity: vicia faba, lupinus albus, and pisum sativum. the nirk gene and transcripts could be detected in all the rhizosphere samples. in contrast, nirs could not be detected. sampling variations were analyzed by comparing denaturing gradient gel electrophoresis ...200515812032
involvement of mitogen-activated protein kinases in the symbiosis bradyrhizobium-lupinus.in plants, mitogen-activated protein kinases (mapks) are involved in signalling to hormones, cell cycle regulation, stresses, and plant defence responses. in this work, several mapks were detected by immunobloting in roots and nodules of lupinus albus produced by inoculation with bradyrhizobium sp. (lupinus). in vitro kinase assays showed that inoculation of seedling roots with b. sp. (lupinus) activates salt stress-inducible and stress-activated mapks after 5 min of incubation. by contrast, ino ...200616868044
assessment of lupin allergenicity in the cholera toxin model: induction of ige response depends on the intrinsic properties of the conglutins and matrix effects.the well-established murine model of ige-mediated food allergy, based on oral administration of antigen and cholera toxin (ct), has within the previous years been used to evaluate various food proteins. nonetheless, little knowledge on the factors that determine the allergenicity of food proteins is available so far. the use of proteins from the legume seed lupinus albus as food ingredients calls for an evaluation of their allergenic potential, and therefore, we applied the cited model to invest ...200616864993
nutritional evaluation of protein, phosphorus, calcium and magnesium bioavailability from lupin (lupinus albus var. multolupa)-based diets in growing rats: effect of alpha-galactoside oligosaccharide extraction and phytase supplementation.the nutritional composition of the legume lupinus albus var. multolupa, raw or after alpha-galactoside extraction, and its effect on the bioavailability of protein, p, ca, and mg by growing rats was evaluated using a balance technique. the protein and dietary fibre content of the lupin flours studied was high, and 89-94% of the dietary fibre was present as insoluble dietary fibre. the alpha-galactoside extraction process did not disrupt the nutritional quality of protein, and the digestive and m ...200616768832
the use of medicinal herbs by diabetic jordanian patients.diabetes mellitus is the most common metabolic disorder worldwide. to date, there have been no reports on the frequency of use of herb medicines in the managements of diabetes mellitus in jordan. this cross-sectional study was conducted by interviewing 310 diabetic patients visiting two medical centers in jordan: jordan university of science & technology medical center and sarih medical center between december 2003 and august 2004. it is found that 31% of interviewed patients have used herbal pr ...200617182483
lupin (lupinus albus) protein isolate (l-iso) has adequate nutritional value and reduces large intestinal weight in rats after restricted and ad libitum feeding.a protein isolate from white lupin (lupinus albus; l-iso) has potential as a novel human food ingredient, but its nutritional effects are unknown.200617191026
effects of extracts of lupine seed on blood glucose levels in glucose resistant mice: antihyperglycemic effects of lupinus albus (white lupine, egypt) and lupinus caudatus (tailcup lupine, mesa verde national park).lupine is a medicinal food plant with potential value in the management of diabetes. in white mice, extracts of seeds of the white lupine [lupinus albus (l. termis l.)] were associated with increased tolerance to an oral glucose bolus. antihyperglycemic activity was present in extracts of the whole seed but not extracts of the seed coat, and was not detected when glucose was administered intraperitoneally rather than orally. however, in contrast to results seen with the prescription drug, acarbo ...200617317651
influence of citric acid amendments on the availability of weathered pcbs to plant and earthworm species.a series of small and large pot trials were conducted to assess the phytoextraction potential of several plant species for weathered polychlorinated biphenyls (pcbs) in soil (105 microg/g arochlor 1268). in addition, the effect of citric acid on pcb bioavailability to both plants and earthworms was assessed. under small pot conditions (one plant, 400 g soil), three cucurbits (cucurbita pepo ssp pepo [zucchini] and ssp ovifera [nonzucchini summer squash], cucumis sativus, cucumber) accumulated up ...200616615308
carboxylate composition of root exudates does not relate consistently to a crop species' ability to use phosphorus from aluminium, iron or calcium phosphate sources.* the relationship between carboxylate release from roots and the ability of the species to utilize phosphorus from sparingly soluble forms was studied by comparing triticum aestivum, brassica napus, cicer arietinum, pisum sativum, lupinus albus, lupinus angustifolius and lupinus cosentinii. * plants were grown in sand and supplied with 40 mg p kg(-1) in the sparingly soluble forms alpo(4), fepo(4) or ca(5)oh(po(4))(3), or as soluble kh(2)po(4); control plants received no p. * the ability to uti ...200717176404
conformation of cytoskeletal elements during the division of infected lupinus albus l. nodule cells.lupin nodule cells maintain their ability to divide for several cycles after being infected by endosymbiotic rhizobia. the conformation of the cytoskeletal elements of nodule cells was studied by fluorescence labelling, immunocytochemistry, and laser confocal and transmission electron microscopy. the dividing infected cells showed the normal microtubule and actin patterns of dividing plant cells. the clustered symbiosomes were tethered to the spindle-pole regions and moved to the cell poles duri ...200717525079
dietary lupin protein lowers triglyceride concentrations in liver and plasma in rats by reducing hepatic gene expression of sterol regulatory element-binding protein-1c.recently, it has been shown that dietary lupin protein lowers plasma triglyceride concentrations in rats. in this study, we investigated the hypothesis that this effect is due to a downregulation of sterol regulatory element-binding protein (srebp)-1c, a transcription factor that regulates the expression of lipogenic enzymes in the livers of rats.200717785965
combined 2d electrophoretic approaches for the study of white lupin mature seed storage proteome.seed proteome analysis by 2d ief/sds-page techniques is challenging for the intrinsic difficulties related to quantitative disparity of the seed proteins, i.e. storage and non-storage proteins, their polymorphic nature, the extensive post-translational modifications and the paucity of deposited primary structures available. conversely, 2d maps of seed proteomes can be extremely useful for a number of fundamental and applied investigations. in this work, we have used a combination of two experime ...200717320919
monoclonal antibodies against the candidate lupin allergens alpha-conglutin and beta-conglutin.the ingestion of dietary products containing sweet lupin (such as lupinus albus or lupinus angustifolius) has been reported to cause ige-mediated allergic reactions. recent studies have indicated lupin globulins as important ige binding proteins. the aim of the present study was to generate and characterize monoclonal antibodies (mabs) against lupin seed proteins.200717192716
cholesterol-lowering effects of dietary lupin (lupinus albus var multolupa) in chicken diets.the present study was undertaken to investigate the effect of different concentrations of lupin seeds (0, 200, and 400 g/kg), with and without cholesterol added (10 g/kg), in chicken diets on performance, relative liver weight, liver fat, intestinal ph and viscosity, and different blood serum parameters (glucose, cholesterol, triglycerides, total biliary salts, amylase, total protein and albumin, and globulin fractions). increasing the lupin content in the diet reduced weight gain and feed consu ...200718029810
lupin protein influences the expression of hepatic genes involved in fatty acid synthesis and triacylglycerol hydrolysis of adult rats.to assess the effect of lupin protein on concentrations of lipids in plasma lipoproteins and liver and hepatic mrna concentrations of genes involved in lipid metabolism, adult rats were fed egg albumin-based diets containing either lupin protein from lupinus albus or casein (50 g/kg) supplemented (hypercholesterolaemic) or not (normolipaemic) with a cholesterol-cholate mixture for 20 d. lupin protein compared with casein lowered the concentrations of tag in liver (p < 0.01) and circulating vldl ...200818096091
hypolipidaemic and anti-atherosclerotic effects of lupin proteins in a rabbit model.the biological activities of a protein isolate from lupin (lupinus albus) were studied in a rabbit model of atherosclerosis. focal plaque development was induced at both common carotid arteries by perivascular injury. after surgery, animals were fed three different diets for 90 d, all with 1 % cholesterol, 15 % sfa and 20 % protein; the protein source was casein (cas), lupin proteins (lup) or 50 % cas+50 % lup (cas+lup). lower cholesterolaemia was detected in the lup v. the cas group at 60 and 9 ...200818315889
proteomic analysis of lupin seed proteins to identify conglutin beta as an allergen, lup an 1.lupin products may be valuable as human foods because of their high protein content and potential anticholesterolemic properties. however, a small percentage of the population is allergic to lupin. in this study, we use in vitro ige binding and mass spectrometry to identify conglutin beta, a major storage protein, as an allergen in seeds of lupinus angustifolius and lupinus albus. purification of conglutin beta from l. angustifolius flour confirmed that serum ige binds to this protein. where ige ...200818620408
sandwich enzyme-linked immunosorbent assay (elisa) for the detection of lupine residues in foods.lupine has been increasingly used in food applications due to its high nutritional value and excellent functional properties. however, lupine provokes allergic reactions in susceptible individuals. the presence of undeclared lupine residues in foods can pose a serious health risk to lupine-allergic individuals. therefore, the objective of this research was to develop a sandwich-type elisa for the detection of lupine residues in foods. lupine flour derived from lupinus albus was used to immunize ...200819019135
identification and characterization of a bowman-birk inhibitor active towards trypsin but not chymotrypsin in lupinus albus seeds.the paper describes the purification, structural characterization and inhibitory properties of a trypsin inhibitor from lupinus albus l., a leguminous plant believed to be devoid of any protease inhibitor. the protein has been isolated by a newly set-up procedure and characterized by direct amino acid sequencing, maldi-tof mass spectroscopy and circular dichroism. inhibitory properties toward bovine trypsin and chymotrypsin, as well as its thermal and ph stabilities, have been also assessed. the ...200818474386
the potential of oil-utilizing bacterial consortia associated with legume root nodules for cleaning oily soils.the surfaces of root nodules of vicia faba and lupinus albus (legume crops), were colonized with bacterial consortia which utilized oil and fixed nitrogen. such combined activities apparently make those periphytic consortia efficient contributors to bioremediation of oily nitrogen-poor desert soils. this was confirmed experimentally in this study. thus, cultivating v. faba, l. albus and, for comparison, solanum melongena, a nonlegume crop, separately in oily sand samples resulted in more oil att ...200919103456
multilocus sequence analysis of the genus bradyrhizobium.the use of multilocus sequence analysis (mlsa) for the taxonomy of bradyrhizobium was assessed. we compared partial sequences for atpd, reca, gyrb, rpob and dnak for a set of reference strains representing named species and genospecies, and a number of new isolates from lupinus albus, arachis hypogaea and ornithopus compressus from spain. the phylogenies of the individual genes were compared with previous dna-dna hybridization results. high hybridization values were well reflected, but intermedi ...200919201125
a label-free internal standard method for the differential analysis of bioactive lupin proteins using nano hplc-chip coupled with ion trap mass spectrometry.quantitative proteomics based on ms is useful for pointing out the differences in some food proteomes relevant to human nutrition. stable isotope label-free (sif) techniques are suitable for comparing an unlimited number of samples by the use of relatively simple experimental workflows. we have developed an internal standard label-free method based on the intensities of peptide precursor ions from ms/ms spectra, collected in data dependent runs, for the simultaneous qualitative characterization ...200919105171
comparative analysis of the contribution of phytochelatins to cadmium and arsenic tolerance in soybean and white lupin.the biosynthesis of phytochelatins (pcs) plays a crucial role in the detoxification and homeostasis of heavy metals and metalloids in plants. however, in an increasing number of plant species metal(loid) tolerance is not well correlated with the accumulation of pcs: tolerant ecotypes frequently contain lower levels of pcs than non-tolerant ecotypes. in this study we have compared the responses of soybean (glycine max l. cv. resnik) and white lupin (lupinus albus l. cv. marta) to cadmium and arse ...200919006673
molecular analysis of scarecrow genes expressed in white lupin cluster roots.the scarecrow (scr) transcription factor plays a crucial role in root cell radial patterning and is required for maintenance of the quiescent centre and differentiation of the endodermis. in response to phosphorus (p) deficiency, white lupin (lupinus albus l.) root surface area increases some 50-fold to 70-fold due to the development of cluster (proteoid) roots. previously it was reported that scr-like expressed sequence tags (ests) were expressed during early cluster root development. here the ...201020167612
nutritive evaluation of legume seeds for ruminant feeding.chemical composition, rumen degradability and the effect of particle losses, and intestinal digestibility of protein by using in situ-in vitro and in vitro techniques were stated for beans (vicia faba), lupin (lupinus albus), vetch (vicia sativa) and bitter vetch (vicia ervilia) and four diets including those legume seeds. in addition, the apparent digestibility of experimental diets was determined in goats. the legume seeds showed high protein content (206-319 g/kg dry matter). effective degrad ...201019138343
strains nodulating lupinus albus on different continents belong to several new chromosomal and symbiotic lineages within bradyrhizobium.in this work we analysed different chromosomal and symbiotic markers in rhizobial strains nodulating lupinus albus (white lupin) in several continents. collectively the analysis of their rrs and atpd genes, and 16s-23s intergenic spacers (its), showed that they belong to at least four chromosomal lineages within the genus bradyrhizobium. most isolates from the canary islands (near to the african continent) grouped with some strains isolated on mainland spain and were identified as bradyrhizobium ...201020135225
effect of feeding growing-fattening rabbits a diet supplemented with whole white lupin (lupinus albus cv. amiga) seeds on fatty acid composition and indexes related to human health in hind leg meat and perirenal fat.a total of 20 weaned rabbits (33 days old) (10 per treatment) were fed one of two diets that included 150 g of sunflower meal (sf)/kg of diet or 120 g of whole white lupin (wl)/kg of diet for 42 days. the wl diet contained less saturated fatty acids (sfa) and polyunsaturated fatty acids (pufa) but more monounsaturated fatty acids (mufa) than the sf diet. the wl diet significantly decreased sfa and pufa content, as well as the pufa n-6/pufa n-3 ratio and saturation, atherogenic and thrombogenic i ...201020864262
interaction between beet vinasse and iron fertilisers in the prevention of iron deficiency in lupins.recycling of organic byproducts for use as soil amendments or fertilisers may enhance the productivity of soils. the aim of this study was to investigate the potential of sugar beet vinasse to correct iron chlorosis in crops when applied in conjunction with fe fertilisers such as vivianite and ferrous sulfate (fs). an experiment involving two factors (fe source and dialysed sugar beet vinasse (dbv) rate) was performed using white lupin (lupinus albus l.) and calcareous sand as growing medium.201020607794
an inhibitor from lupinus bogotensis seeds effective against aspartic proteases from hypothenemus hampei.the coffee berry borer, hypothenemus hampei (ferrari), is one of the most devastating coffee pests (coffea arabica l.) worldwide. digestion in the midgut of h. hampei is facilitated by aspartic proteases. this is the first report of an aspartic protease inhibitor from lupinus bogotensis. the l. bogotensis aspartic protease inhibitor (lbapi) exhibited a molecular mass of 12.84kda, as determined by maldi-tof, and consists of a single polypeptide chain with an isoelectric point of 4.5. in thermal a ...201020347105
phytoremediation of pharmaceuticals--preliminary study.phytoremediation of selected pharmaceuticals (diclofenac, ibuprofen, and acetaminophen) using armoracia rusticana and linum usitatissimum cell cultures and by hydroponically cultivated lupinus albus, hordeum vulgaris, and phragmites australis plants in laboratory conditions is described. during in vitro experiments, the best results for acetaminophen were achieved using armoracia rusticana hairy root cultures, where 100% of the starting amount was removed from the media during eight days. total ...201020734624
three-dimensional visualization and quantification of water content in the rhizosphere.• despite the importance of rhizosphere properties for water flow from soil to roots, there is limited quantitative information on the distribution of water in the rhizosphere of plants. • here, we used neutron tomography to quantify and visualize the water content in the rhizosphere of the plant species chickpea (cicer arietinum), white lupin (lupinus albus), and maize (zea mays) 12 d after planting. • we clearly observed increasing soil water contents (θ) towards the root surface for all three ...201121824150
boron accumulation and toxicity in hybrid poplar (populus nigra × euramericana).poplars accumulate high b concentrations and are thus used for the phytomanagement of b contaminated soils. here, we performed pot experiments in which populus nigra × euramericana were grown on a substrate with b concentrations ranging from 13 to 280 mg kg(-1) as h(3)bo(3). salix viminalis, brassica juncea, and lupinus albus were grown under some growing conditions for comparison. poplar growth was unaffected at soil b treatment levels up to 93 mg kg(-1). growth was progressively reduced at lev ...201122050628
time and substrate dependent exudation of carboxylates by lupinus albus l. and brassica napus l.root exudates influence significantly physical, chemical and biological characteristics of rhizosphere soil. their qualitative and quantitative composition is affected by environmental factors such as ph, soil type, oxygen status, light intensity, soil temperature, plant growth, nutrient availability and microorganisms. the aim of the present study was to assess the influence of growth substrate and plant age on the release of carboxylates from lupinus albus l. and brassica napus l. both plant s ...201122000050
identification and characterisation of seed storage protein transcripts from lupinus angustifolius.in legumes, seed storage proteins are important for the developing seedling and are an important source of protein for humans and animals. lupinus angustifolius (l.), also known as narrow-leaf lupin (nll) is a grain legume crop that is gaining recognition as a potential human health food as the grain is high in protein and dietary fibre, gluten-free and low in fat and starch.201121457583
bradyrhizobium canariense and bradyrhizobium japonicum are the two dominant rhizobium species in root nodules of lupin and serradella plants growing in europe.forty three bradyrhizobium strains isolated in poland from root nodules of lupin species (lupinus albus, l. angustifolius and l. luteus), and pink serradella (ornithopus sativus) were examined based on phylogenetic analyses of three housekeeping (atpd, glnii and reca) and nodulation (noda) gene sequences. additionally, seven strains originating from root-nodules of yellow serradella (o. compressus) from asinara island (italy) were included in this study. phylogenetic trees revealed that 15 serra ...201121514760
dehulled-micronised lupin (lupinus albus l. cv. multitalia) as the main protein source for broilers: influence on growth performance, carcass traits and meat fatty acid composition.background: a study was carried out to compare the effect of diets containing micronised-dehulled lupin (lupinus albus l. cv. multitalia) on growth performance, carcass yields and meat fatty acid profile of broiler chickens. results: hubbard strain chicks receiving from 14 d to slaughtering age (49 d) a wheat middlings-based diet containing either deffatted soybean meal (195 g kg(-1) , control) or dehulled micromicronised lupins (240 g kg(-1) ) as the main protein source were used. the inclusion ...201121520452
white lupin cluster root acclimation to phosphorus deficiency and root hair development involve unique glycerophosphodiester phosphodiesterases.white lupin (lupinus albus) is a legume that is very efficient in accessing unavailable phosphorus (pi). it develops short, densely clustered tertiary lateral roots (cluster/proteoid roots) in response to pi limitation. in this report, we characterize two glycerophosphodiester phosphodiesterase (gpx-pde) genes (gpx-pde1 and gpx-pde2) from white lupin and propose a role for these two gpx-pdes in root hair growth and development and in a pi stress-induced phospholipid degradation pathway in cluste ...201121464471
effect of a traditional processing method on the chemical composition of local white lupin (lupinus albus l.) seed in north-western ethiopia.the effect of a traditional ethiopian lupin processing method on the chemical composition of lupin seed samples was studied. two sampling districts, namely mecha and sekela, representing the mid- and high-altitude areas of north-western ethiopia, respectively, were randomly selected. different types of traditionally processed and marketed lupin seed samples (raw, roasted, and finished) were collected in six replications from each district. raw samples are unprocessed, and roasted samples are roa ...201121950165
comparative analyses of three legume species reveals conserved and unique root extracellular proteins.increasing evidence suggests that root extracellular proteins are involved in interactions between roots and their soil environment. in the present study, exudates released by 6-day-old roots of the three legume species white lupin (lupinus albus), soybean (glycine max), and cowpea (vigna sinensis) were collected under axenic conditions, and their constitutively secreted proteomes were analyzed. between 42 and 93 unique root extracellular proteins with 2 or more different peptide fragments per p ...201222945350
characterization of an isoflavonoid-specific prenyltransferase from lupinus albus.prenylated flavonoids and isoflavonoids possess antimicrobial activity against fungal pathogens of plants. however, only a few plant flavonoid and isoflavonoid prenyltransferase genes have been identified to date. in this study, an isoflavonoid prenyltransferase gene, designated as lapt1, was identified from white lupin (lupinus albus). the deduced protein sequence of lapt1 shared high homologies with known flavonoid and isoflavonoid prenyltransferases. the lapt1 gene was mainly expressed in roo ...201222430842
the bacteriological safety and antimicrobial susceptibility of bacteria isolated from street-vended white lupin (lupinus albus) in bahir dar, ethiopia.whereas street vended foods are readily available sources of meals for many people across the world, the microbial safety of such food is always uncertain. in developing countries the major sources of food-borne illnesses are street vended foods. the aim of this study was thus to assess the prevalence and antibiogram of bacteria from white lupin in bahir dar town.201323559834
[development of a high content protein beverage from chilean mesquite, lupine and quinoa for the diet of pre-schoolers].this research was aimed at developing a high content protein beverage from the mixture of liquid extracts of a pseudocereal, quinoa (chenopodium quinoa willd) and two legumes: mesquite (prosopis chilensis (mol.) stunz) and lupine (lupinus albus l.), native from the andean highlands of the chilean northern macro-zone, flavored with raspberry pulp, to help in the feeding of children between 2 and 5 years of lower socioeconomic status with nutritional deficiencies. the formulation was defined by li ...201322566327
lupinus albus plants acquire mercury tolerance when inoculated with an hg-resistant bradyrhizobium strain.one strain of bradyrhizobium canariense (l-7ah) was selected for its metal-resistance and ability to nodulate white lupin (lupinus albus l.) plants, from a collection of rhizobial strains previously created from soils of the almadén mining district (spain) with varying levels of hg contamination. plants were inoculated with either strain l-7ah (hg-tolerant) or l-3 (hg-sensitive, used as control), and watered with nutrient solutions supplemented with various concentrations (0-200 μm) of hgcl2 in ...201324125840
macro- and microelement contents of some legume seeds.macro- and microelement contents of legume seeds were determined by icp-aes. the potassium k contents of seeds ranged between 7,426 mg/kg (lupinus albus l.) and 16,558 mg/kg (phaseolus vulgaris l. ssp. sphaericus mart). in addition, while p contents of seeds changed from 2,719 mg/kg (l. albus l.) to 5,792 mg/kg (vigna sinensis (l.) savi), ca contents ranged at the levels between 1,309 mg/kg (cicer arietinum l.) and 2,781 mg/kg (l. albus l.). the microelement contents of samples were found to be ...201323715731
an rna-seq transcriptome analysis of orthophosphate-deficient white lupin reveals novel insights into phosphorus acclimation in plants.phosphorus, in its orthophosphate form (p(i)), is one of the most limiting macronutrients in soils for plant growth and development. however, the whole-genome molecular mechanisms contributing to plant acclimation to p(i) deficiency remain largely unknown. white lupin (lupinus albus) has evolved unique adaptations for growth in p(i)-deficient soils, including the development of cluster roots to increase root surface area. in this study, we utilized rna-seq technology to assess global gene expres ...201323197803
structural analysis and profiling of phenolic secondary metabolites of mexican lupine species using lc-ms techniques.flavonoid glycoconjugates from roots and leaves of eight north america lupine species (lupinus elegans, lupinus exaltatus, lupinus hintonii, lupinus mexicanus, lupinus montanus, lupinus rotundiflorus, lupinus stipulatus, lupinus sp.), three mediterranean species (lupinus albus, lupinus angustifolius, lupinus luteus) and one species from south america domesticated in europe (lupinus mutabilis) were analyzed using two lc/ms systems: low-resolution ion trap instrument and high-resolution quadrupole ...201323642387
paenibacillus lupini sp. nov., isolated from nodules of lupinus albus.a bacterial strain designated rlahu15(t) was isolated from root nodules of lupinus albus in spain. phylogenetic analyses based on 16s rrna gene sequences placed the isolate in the genus paenibacillus, with its closest relatives being paenibacillus catalpae d75(t), paenibacillus glycanilyticus ds-1(t), paenibacillus endophyticus pecae04(t) and paenibacillus xinjiangensis b538(t) with 98.8 %, 98.9 %, 97.4 % and 97.4 % similarity, respectively. dna-dna hybridization studies showed values lower than ...201424928428
cohnella lupini sp. nov., an endophytic bacterium isolated from root nodules of lupinus albus.a bacterial strain designated rlahu4b(t) was isolated from root nodules of lupinus albus in león (spain). the 16s rrna gene sequence of this strain showed similarities lower than 97 % with respect to species of the genus cohnella. the strain was a gram-variable, sporulating rod, motile by means of peritrichous flagella, and facultatively anaerobic. it was positive for oxidase, catalase and β-galactosidase production but negative for urease, amylase and gelatinase. strain rlahu4b(t) grew in the p ...201424021729
legume genomics: understanding biology through dna and rna sequencing.the legume family (leguminosae) consists of approx. 17 000 species. a few of these species, including, but not limited to, phaseolus vulgaris, cicer arietinum and cajanus cajan, are important dietary components, providing protein for approx. 300 million people worldwide. additional species, including soybean (glycine max) and alfalfa (medicago sativa), are important crops utilized mainly in animal feed. in addition, legumes are important contributors to biological nitrogen, forming symbiotic rel ...201424769535
mitigation of cu stress by legume-rhizobium symbiosis in white lupin and soybean plants.the effect of bradyrhizobium-legume symbiosis on plant growth, toxicological variables and cu bioaccumulation was studied in white lupin and soybean plants treated with 1.6, 48, 96 and 192 μm cu. in both species, those plants grown in the presence of root nodule-forming symbiotic bradyrhizobium showed less root and shoot growth reduction, plus greater translocation of cu to the shoot, than those grown without symbiotic bradyrhizobium. the effective added concentrations of cu that reduced shoot a ...201424580814
synchrotron-based techniques shed light on mechanisms of plant sensitivity and tolerance to high manganese in the root environment.plant species differ in response to high available manganese (mn), but the mechanisms of sensitivity and tolerance are poorly understood. in solution culture, greater than or equal to 30 µm mn decreased the growth of soybean (glycine max), but white lupin (lupinus albus), narrow-leafed lupin (lupin angustifolius), and sunflower (helianthus annuus) grew well at 100 µm mn. differences in species' tolerance to high mn could not be explained simply by differences in root, stem, or leaf mn status, be ...201526395840
impact of biochar and root-induced changes on metal dynamics in the rhizosphere of agrostis capillaris and lupinus albus.rhizosphere interactions are deemed to play a key role in the success of phytoremediation technologies. here, the effects of biochar and root-induced changes in the rhizosphere of agrostis capillaris l. and lupinus albus l. on metal (cd, pb and zn) dynamics were investigated using a biotest on a 2mm soil layer and a sequential extraction procedure (tessier's scheme). in the bulk soil, the application of 5% biochar significantly reduced the exchangeable pool of metals primarily due to a liming ef ...201525559173
in vitro fermentation of lupin seeds (lupinus albus) and broad beans (vicia faba): dynamic modulation of the intestinal microbiota and metabolomic output.broad beans (vicia faba) and lupin seeds (lupinus albus) are legumes rich in a wide range of compounds, which may represent a useful dietary approach for modulating the human gut microbiome. in this work, after in vitro digestion, legume samples were used as carbon sources in anaerobic batch cultures to evaluate their impact on the intestinal microbiota composition and on their metabolic products. the fermentations were monitored by a decrease in ph, generation of short chain fatty acids (scfa) ...201526252418
effects of different forms of white lupin (lupinus albus) grain supplementation on feed intake, digestibility, growth performance and carcass characteristics of washera sheep fed rhodes grass (chloris gayana) hay-based diets.protein is the major limiting nutrient in feeding ruminants especially in dryland areas. thus, looking for locally available protein sources such as white lupin (lupinus albus) grain is commendable. the objective of this experiment was to determine effects of supplementation of different forms of white lupin grain (wlg) on feed and nutrient intake, digestibility, growth and carcass characteristics. twenty-five yearling male washera sheep with initial body weight (bw) of 16.26 ± 1.41 kg (mean ± s ...201526250152
rhizosphere priming effect on soil organic carbon decomposition under plant species differing in soil acidification and root exudation.effects of rhizosphere properties on the rhizosphere priming effect (rpe) are unknown. this study aimed to link species variation in rpe with plant traits and rhizosphere properties. four c3 species (chickpea, cicer arietinum; field pea, pisum sativum; wheat, triticum aestivum; and white lupin, lupinus albus) differing in soil acidification and root exudation, were grown in a c4 soil. the co2 released from soil was trapped using a newly developed naoh-trapping system. white lupin and wheat showe ...201627101777
effects of intercropping of oat (avena sativa l.) with white lupin (lupinus albus l.) on the mobility of target elements for phytoremediation and phytomining in soil solution.this study aims to investigate how intercropping of oat (avena sativa l.) with white lupin (lupinus albus l.) affects the mobile fractions of trace metals (fe, mn, pb, cd, th, u, sc, la, nd, ge) in soil solution. oat and white lupin were cultivated in monocultures and mixed cultures with differing oat/white lupin ratios (11% and 33% lupin, respectively). temporal variation of soil solution chemistry was compared with the mobilization of elements in the rhizosphere of white lupin and concentratio ...201626940160
the effect of lupinus albus seeds on digestibility, performance and gastrointestinal tract indices in pigs.the effect of soya bean meal (sbm) substitution in the diet with white lupine (wl) seeds on performances and some parameters of the digestive tract of pigs were studied. the digestibility experiment was performed on 30 pigs using difference method combined with marker method, where one group was offered basal diet and in diet of experimental groups 25% of the basal diet was substituted with sbm or wl meal. the growth experiment was conducted on 48 pigs of approximately 20 kg body weight. the ani ...201627868253
nitrogen transfer from lupinus albus l., trifolium incarnatum l. and vicia sativa l. contribute differently to rapeseed (brassica napus l.) nitrogen nutrition.nitrogen (n) transfer is well documented in legume-cereal intercropping but this is less often reported for legume-brassica intercrops even though brassica crops require higher levels of n fertilizers. the present study was carried out to quantify n transfer from legumes (lupinus albus l., trifolium incarnatum l. or vicia sativa l.) to rapeseed (brassica napus l.) using the split-root (15)n-labelling method. after three months we observed that legumes did not alter the growth of rapeseed. vetch ...201627656683
clarification on host range of didymella pinodes the causal agent of pea ascochyta blight.didymella pinodes is the principal causal agent of ascochyta blight, one of the most important fungal diseases of pea (pisum sativum) worldwide. understanding its host specificity has crucial implications in epidemiology and management; however, this has not been clearly delineated yet. in this study we attempt to clarify the host range of d. pinodes and to compare it with that of other close didymella spp. d. pinodes was very virulent on pea accessions, although differences in virulence were id ...201627242812
total growth and root-cluster production by legumes and proteas depends on rhizobacterial strain, host species and nitrogen level.root clusters are bunches of hairy rootlets produced by >1800 species in nine families. the possible involvement of micro-organisms in root-cluster formation has produced conflicting results over the last 40 years. in addition, any effect of rhizobacteria on overall plant growth of root-cluster-bearing species remains unknown.201627288511
compositional changes in (iso)flavonoids and estrogenic activity of three edible lupinus species by germination and rhizopus-elicitation.the effects of germination and elicitation on (iso)flavonoid composition of extracts from three edible lupine species (lupinus luteus, lupinus albus, lupinus angustifolius) were determined by rp-uhplc-ms(n). the total (iso)flavonoid content of lupine increased over 10-fold upon germination, with the total content and composition of isoflavonoids more affected than those of flavonoids. glycosylated isoflavones were the most predominant compounds found in lupine seedlings. lesser amounts of isofla ...201626749476
blad-containing oligomer fungicidal activity on human pathogenic yeasts. from the outside to the inside of the target cell.blad polypeptide comprises residues 109-281 of lupinus albus β-conglutin precursor. it occurs naturally as a major subunit of an edible, 210 kda oligomer which accumulates to high levels, exclusively in the cotyledons of lupinus seedlings between the 4th and 14th day after the onset of germination. blad-containing oligomer (bco) exhibits a potent and broad spectrum fungicide activity toward plant pathogens and is now on sale in the us under the tradename fracture(tm). in this work we demonstrate ...201627933037
major crop species show differential balance between root morphological and physiological responses to variable phosphorus supply.the relationship between root morphological and physiological responses to variable p supply in different plant species is poorly understood. we compared root morphological and physiological responses to p supply in seven crop species (zea mays, triticum aestivum, brassica napus, lupinus albus, glycine max, vicia faba, cicer arietinum) treated with or without 100 mg p kg(-1) in two soils (acidic and calcareous). phosphorus deficiency decreased root length more in fibrous root species (zea mays, ...201628066491
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