Publications

properties of a beta-(1-4)-glucan hydrolase from aspergillus niger.	1. a beta-(1-->4)-glucan hydrolase prepared from aspergillus niger, as described by clarke & stone (1965a), showed a ph optimum in the range 4.5-6 and k(m) 0.25% when acting on a cellulose dextrin sulphate substrate. 2. the hydrolase rapidly decreased the specific viscosity of carboxymethylcellulose with a small increase in the production of reducing sugars. the identity of the products of hydrolysis of cellotetraose, cellopentaose and their reduced analogues indicate a preferential cleavage of ...	1965	5862418
determination of linkages of beta-d-glucans from lupinus albus and avena sativa by exo-beta-(1 --> 3)-d-glucanase.		1970	16657310
[the lupine, a contribution to nutrition in the andes. 2. the quality of proteins in lupinus mutabilis compared to lupinus albus, lupinus luteus and soja max].		1975	1224691
[lupine, a contribution to the human food supply. 3. nutritional physiological study with lupine (lupinus albus) flour].	lupinus albus flour doses of 58.9 +/- 9.6 g have been given to 20 persons. this sweet lupine flour has been digestible without complications in all cases. there have been no significant changes of hemoglobine, hematocrite, total protein, urea, bilirubine, and sgot in the blood. so the lupine may be used for improvement of protein supply in men under the criteria that (1) the alkaloid content of the seed does not exceed 0.02%, (2) the seed itself contains no secondary fungi which may cause a lupi ...	1976	1032959
[lupine, a contribution to the human food supply. 4. acceptability of lupinus alba flour].	48 different dishes enriched with lupinus albus flour have been tested by 20 persons. the test duration has been 4 weeks. meals and products with lupine admixtures have been judged equally with concern to corresponding conventional dishes. farinaceous products had the best test results. therefore, it is recommended for peru to add lupine flour when producing bread, biscuits, sauces, soups, and noodles (maccaronis etc.). the test time had no influence to the test results.	1976	1020375
red light-enhanced phytochrome pelletability: re-examination and further characterization.	red light-enhanced pelletability of phytochrome was observed in extracts of all 11 plants tested: avena sativa l., secale cereale l., zea mays l., cucurbita pepo l., sinapis alba l., pisum sativum l., helianthus anuus l., raphanus sativus l., glycine max (l.) merr., phaseolus vulgaris l., and lupinus albus l. this enhanced pelletability was observed in all 11 plants following in situ irradiation (in vivo binding) but only in sinapis and cucurbita after irradiation of crude extracts (in vitro bin ...	1976	16659745
economy of photosynthate use in nitrogen-fixing legume nodules: observations on two contrasting symbioses.	the economy of c use by root nodules was examined in two symbioses, vigna unguiculata (l.) walp. (cv. caloona):rhizobium cb756 and lupinus albus l. (cv. ultra):rhizobium wu425 over a 2-week period in early vegetative growth. plants were grown in minus n water culture with cuvettes attached to the nodulated zone of their primary roots for collection of evolved co(2) and h(2). increments in total plant n and in c and n of nodules, and c:n weight ratios of xylem and phloem exudates were studied by ...	1979	16661076
[lupine seed meal (lupinus albus cult. astra) as a protein source in swine rations for growth and fattening].		1979	116448
[nutritive value of lupine and its potential as human food].	the chemical composition and some indices of protein quality were measured in two species of sweet lupine lupinus albus and lupinus luteus grown at the experimental station in gorbea, chile: both samples showed a high protein content (39.5 and 44.6%). the protein efficiency ratio (per) was measured in the rat and found to be 0.48 and 0.71, respectively, as compared to 2.57 for casein. supplementation with 0.3% dl-methionine increased significantly those values, thus indicating that lupine protei ...	1979	550741
chemical composition, nutritive value, and toxicological evaluation of two species of sweet lupine (lupinus albus and lupinus luteus).		1980	7391375
[effectiveness of symbiotic n2-fixation in leguminous plants, as affected by inoculation with rhizobia, by substrate, n-fertilizing, and 14c-sucrose application (author's transl)].	cultivation experiments (mitscherlich-vessels, quartz sand, 15n-labelled soil, 15n-fertilizer) showed, that various strains of rhizobium lupini (white and yellow lupines) and of rhizobium leguminosarum (field beans and peas) induced a different n2-fixation of the inoculated plants, the most effective rhizobium strains being 367a, cz, t3, 271 (rh. lupini), and azotogen (rh, leguminosarum). yellow lupines and field bean plants were supplied with n2 from the air considerably better than white lupin ...	1980	6252716
[topography of the metabolic cycle of 4-aminobutyrate].	this work describes, with some detail the intervention of 4-aminobutyrate as protagonist of a derivation of tricarboxylic cycle. its vicarial mission is emphasized in connection with its existence in microorganisms (escherichia coli and pseudomonas fluorescens), plants (helianthus tuberosus. lupinus albus and agave americana), neoplasic cells (ascitic tumor of ehrlich and hela cells) and animal tissues (adrenal medulla and brain.	1982	6815735
de novo purine synthesis in nitrogen-fixing nodules of cowpea (vigna unguiculata [l.] walp.) and soybean (glycine max [l.] merr.).	partially purified, cell-free extracts from nodules of cowpea (vigna unguiculata l. walp. cv. caloona) and soybean (glycine max l. merr. cv. bragg) showed high rates of de novo purine nucleotide and purine base synthesis. activity increased with rates of nitrogen fixation and ureide export during development of cowpea plants; maximum rates (equivalent to 1.2 micromoles n(2) per hour per gram fresh nodule) being similar to those of maximum nitrogen fixation (1-2 micromoles n(2) per hour per gram ...	1982	16662479
chemical and nutritional evaluation of sweet lupines.	sweet lupines are presently being cultivated at the experimental station in gorbea (chile). four cultivars, lupinus albus cv astra, l. albus cv multolupa, lupinus luteus cv aurea and lupinus angustifolius, were examined for the chemical composition of whole and dehulled seeds and testa. whole seeds contain 34-35% protein. the varieties of l. albus contain 13% oil. l. luteus and l. angustifolius contain about 5% ether extract. crude fiber ranged from 11 to 17% in the whole seed. unsaturated fatty ...	1983	6651228
safety evaluation of sweet lupine (lupinus albus cv. multolupa). ii. nine-month feeding and multigeneration study in rats.	sweet lupine lupinus albus cv. multolupa) flour containing 41.7% protein and 0.025% lupanine was fed for 9 months to two generations of rats (f1 and f2) at a level that provided 20% dietary protein. the diets were supplemented with 0.2% dl-methionine. the control diet provided the same dietary protein level from defatted soya-bean flour, fish meal and dried skimmed milk. the lupine diet had no effect on the general condition, mortality or behaviour of the animals. the growth rate of males fed sw ...	1984	6537936
comparative pharmacological study of sparteine and its ketonic derivative lupanine from seeds of lupinus albus.		1984	6151710
effects of n(2) deficiency on transport and partitioning of c and n in a nodulated legume.	nodulated root systems of white lupin (lupinus albus l. cv ultra: rhizobium strain wu425) were exposed to ar:o(2) (80:20, v/v) or ar:n(2):o(2) (70:10:20, v/v/v) and c and n partitioning were examined over a 9- or 10-day period in comparison with control plants with nodulated roots retained in air. accumulation of n ceased in plants exposed to ar:o(2) or was much reduced in plants exposed to ar:n(2):o(2), but net c assimilation rates and profiles of c utilization remained similar to those of cont ...	1984	16663823
effects of short-term n(2) deficiency on n metabolism in legume nodules.	the study aimed to test the hypothesis that ammonia production by rhizobium bacteroids provides not only a source of nitrogen for growth but has a central regulatory role in maintaining the metabolic activity and functional integrity of the legume nodule. production of ammonia in intact, attached nodules was interrupted by short-term (up to 3 days) exposure of the nodulated root systems of cowpea (vigna unguiculata l. walp cv vita 3: rhizobium cb 756) and lupin (lupinus albus l. cv ultra: rhizob ...	1984	16663910
digestion of polysaccharides, protein and lipids by adult cockerels fed on diets containing a pectic cell-wall material from white lupin (lupinus albus l.) cotyledon.	1. the cell-wall material of white lupin (lupinus albus l.) cotyledon is characterized by low contents of cellulose (47 g/kg) and lignin (17 g/kg) and a high content of pectic substances (710 g/kg). the digestion of lupin cell-wall material by adult cockerels was estimated using gas-liquid chromatographic analyses of alditol acetates derived from polysaccharide sugars. the analyses were performed in the destarched water-insoluble fractions of feed and excreta. digestibility measurements were car ...	1985	2825761
nutritional value of lupin (lupinus albus)-seed meal for growing pigs: availability of lysine, effect of autoclaving and net energy content.	1. two experiments were conducted to assess the nutritional value of lupin (lupinus albus)-seed meal for growing pigs. in the first, the availability of lysine was assessed using slope-ratio analysis. in the second, the effects of autoclaving lupin seeds and formulating the diets on the basis of estimated digestible or net energy were assessed. 2. in the first experiment, the availability of lysine in three samples of lupin-seed meal was compared with that in meat-and-bone meal and soya-bean mea ...	1986	3118937
white lupin as a protein source for layers.	three feeding experiments were conducted with single comb white leghorn hens to determine the effects of lupinus albus cv. ultra on performance. raw, autoclaved, and extruded lupins were fed at 10 and 20% of the diet for 12 wk in experiment 1. in experiment 2 raw and extruded lupins were fed at 10, 15, 20, and 25% of the diet for 16 wk. raw lupin was fed for 32 wk at 10, 15, 20, 25, and 30% of the diet in experiment 3. average egg weight, egg production (percent hen-day basis), and feed consumpt ...	1987	3447138
effects of white lupine in diets of growing turkeys.	in experiment 1, dehulling white lupine (wl) improved growth and feed efficiency of male large white turkey poults fed 40% wl during 4 to 21 days of age. in experiment 2, wl was included proportionally with soybean meal in corn-soy diets of female turkeys from 0 to 17 wk of age. soybean meal: wl ratios (s:wl) were 1:0 (control), 2:1, 1:1, and 6:1. at 17 wk of age hens on s:wl diets of 2:1 had body weights that were similar to those of birds fed the corn-soy control diets. growth of hens fed s:wl ...	1988	3405936
[aspartyladenylate analog--effective inhibitor of asparagine synthetase].	a number of earlier unknown phosphonate analogues of aspartyl adenylate with anhydride oxygen substituted by --ch2--, and the carbonyl group substituted by --ch(oh)- or --ch(nh2)-groups were synthesized. these compounds were used to study the reaction mechanism of asparagine synthetases from white lupine and e. coli. the aspartyl adenylate analogues proved to be powerful competitive inhibitors (ki = 10(-7) m) of the bacterial enzyme. in the case of white lupine enzyme catalyzing the aspartate-in ...	1988	2903747
occurrence of h(2)-uptake hydrogenases in bradyrhizobium sp. (lupinus) and their expression in nodules of lupinus spp. and ornithopus compressus.	fifty-four strains of bradyrhizobium sp. (lupinus) from worldwide collections were screened by a colony hybridization method for the presence of dna sequences homologous to the structural genes of the bradyrhizobium japonicum hydrogenase. twelve strains exhibited strong colony hybridization signals, and subsequent southern blot hybridization experiments showed that they fell into two different groups on the basis of the pattern of ecori fragments containing the homology to the hup probe. all str ...	1989	16666550
leghemoglobin in lupin plants (lupinus albus cv multolupa).	leghemoglobin was localized by immunogold techniques in nodules of lupinus albus cv multolupa inoculated with bradyrhizobium sp. (lupinus) strain islu 16. the protein localization was performed in nodules embedded in spurr's and araldite epoxy resins and lowycryl k4m. a very good preservation of both the ultrastructure and antigenicity was obtained with the tissues embedded in araldite following glutaraldehyde fixation and unpostfixed in osmium tetroxide. lupin leghemoglobin is a stable and abun ...	1989	16666792
evaluation of white lupines and triticale in calf starter diets.	one hundred fifty-two holstein calves were used to evaluate white lupines (lupinus albus cv. ultra) and triticale in starter diets. the basal diet contained corn, oats, minerals, and vitamins. starter components were 1) 14% soybean meal; 2) 10% soybean meal and 27% triticale; 3) 22% lupines; 4) 16% lupines and 24.5% triticale; 5) 11% lupines and 8% soybean meal; and 6) 7% lupines, 5% soybean meal, and 26.5% triticale. dietary cp was approximately 16% of dm. data collection began on d 4 after bir ...	1989	2745803
lupin as a protein supplement for growing lambs.	whole lupins (lupinus albus) were roasted with exit temperatures ranging from approximately 130 (moderate heat) to 175 degrees c (high heat). in situ n disappearance after 12 h of incubation in the rumen was 83% for raw lupins, 45% for lupins roasted at moderate temperatures, and 39% for lupins roasted at high temperatures. lambs fed lupins roasted at moderate temperatures retained more n (p less than .01) than those fed soybean meal (sbm). however, growth rate and feed efficiency were similar a ...	1991	1894575
nutritional quality of lupine (lupinus albus cv. multolupa) as affected by lactic acid fermentation.	the effects of selected nrrl strains of lactobacillus acidophilus, l. buchneri, l. cellobiosus and l. fermentum upon oligosaccharide, phytate and alkaloid contents, as well as on the nutritive value of lupine, were investigated. lupine was processed to a 12% total solids suspension, inoculated with 1% (v/v) cultures and fermented until a final desired ph of 4.5. l. acidophilus b-2092 and l. buchneri b-1837 growth was related to a significant sucrose breakdown and decreases of phytates, whereas l ...	1991	1790104
[ruminal digestion and intestinal absorption of lupine proteins extruded in the lactating cow].	four lactating cows fitted with permanent ruminal, duodenal and ileal cannulae were used to study the effect of extrusion of whole lupin seeds at 195 degrees c (lupinus albus cv lublanc) on organic matter (om) and nitrogen (n) degradation in the rumen and their flow to and absorption from the small intestine. raw whole lupin seeds (rwls) and extruded whole lupin seeds (ewls) were fed in diets containing 15.5% crude protein and composed of 22.6% whole lupin seeds, 56.5% corn silage, 10.2% corn gr ...	1991	1777057
in situ evaluation of the ruminal and intestinal degradability of extruded whole lupin seed nitrogen.	the effect of whole lupin seeds (lupinus albus cv lublanc) at 120, 150 and 195 degrees c on in situ nitrogen degradability (dg.n) was measured by the nylon bag technique using fistulated non-lactating holstein cows. the n degradation was evaluated in nylon bags suspended in the rumen; heating the seeds at 120, 150 and 195 degrees c decreased the dg.n value: 83.9, 72.9 and 53.0 respectively vs 95.3% (rumen outflow rate of 0.06/h). to estimate the total n disappearing in the digestive tract, bags ...	1991	1768316
a calluna vulgaris extract 5-lipoxygenase inhibitor shows potent antiproliferative effects on human leukemia hl-60 cells.	a water-calluna vulgaris extract (water-cve) was found to be a relatively specific arachidonate 5-lipoxygenase inhibitor and showed potent anti-proliferative effects on human leukemic hl60 cells. water-cve completely inhibited potato 5-lipoxygenase activity at 250 micrograms/ml, partially inhibited soybean 15-lipoxygenase at ph 7.4 and had no effect either on this 15-lipoxygenase at its optimal activity ph (ph 9) or on lupinus albus 5-, 8-, 15-lipoxygenase. in culture, the proliferation and dna ...	1992	1419080
performance of chickpea, lentil and lupin nodulated with indigenous or inoculated rhizobia micropartners under nitrogen, boron, cobalt and molybdenum fertilization schedules.	chickpea (cicer arietinum), lentil (lens esculenta) and lupin (lupinus albus) responded to inoculation with their respective symbiotants:rhizobium loti, rhizobium leguminosarum biovarviceae andbradyrhizobium sp. (lupinus) alone or with (nh4)2so4 at 30 ppm n. soil application of na2moo4.2h2o at 3 ppm mo, cocl2.6h2o at 2 ppm co(2+) or na2b4o7.10h2o at 1 ppm b with 0 and 30 ppm n increased nodule weight and plant dry weight and n-content 60 days after sowing and seed yield, seed size and the n and ...	1992	24425609
host status of selected crops to meloidogyne chitwoodi.	various crops were tested in greenhouse and field trials for their potential utility in the rotation sequence in the potato cropping system in meloidogyne chitwoodi-infested soils of the klamath basin in northeastern california and southern oregon. two solarium accessions from the international potato center in peru were potential sources of resistance to m. chitwoodi. cultivars of barley, oat, rye, wheat, and white lupine were maintenance hosts, supporting the nematode population at its current ...	1993	19279852
lupins (lupinus albus) as a protein supplement for lactating holstein dairy cows.	three experiments were conducted to determine optimal substitution of lupin for soybean meal in dairy cattle diets; the effects of whole versus ground lupins and the effects of whole lupins versus whole soybeans on milk production were examined. in the first experiment, 57 cows were assigned randomly to treatments in blocks of five by calving order within parity. treatments began 22 d postpartum and continued through d 140. diets were isonitrogenously balanced using soybean meal as control: lupi ...	1993	8227670
effect of enzyme supplementation on the nutritional value of raw, autoclaved, and dehulled lupins (lupinus albus) in chicken diets.	the effects of adding crude enzyme preparations to diets containing raw, autoclaved, and dehulled lupin seeds on the performance of broiler and leghorn chicks (7 to 21 days) were evaluated in five experiments. in the first experiment, enzyme addition (combination of energex-carbohydrase, bio-feed pro-protease, and novozyme-alpha-galactosidase) to a diet containing 70% raw lupins improved the weight gain and feed to gain ratio of broiler chicks by 18 and 10%, respectively. the relative intestinal ...	1993	8309877
the toxicity of seed extracts and their fractions from lupinus angustifolius l. and lupinus albus l.	seed extracts obtained from lupinus albus and lupinus angustifolius by treatment with 48% ethanol contained ca. 10% alkaloids (on a dry weight basis) and were non-toxic. their acute toxicity (ld50) in the mouse is > 4000 mg kg-1 body wt. after fractionation, the extract from l. angustifolius seeds afforded several fractions with differing toxicities (ld50 750-4000 mg kg-1 body wt.). none of the fractions tested in vitro were toxic. the results obtained showed that, in spite of the alkaloids, oth ...	1993	8258632
binding of quinolizidine alkaloids to nicotinic and muscarinic acetylcholine receptors.	fourteen quinolizidine alkaloids, isolated from lupinus albus, l. mutabilis, and anagyris foetida, were analyzed for their affinity for nicotinic and/or muscarinic acetylcholine receptors. of the compounds tested, the alpha-pyridones, n-methylcytisine and cytisine, showed the highest affinities at the nicotinic receptor, while several quinolizidine alkaloid types were especially active at the muscarinic receptor.	1994	7798968
immunotropic activity of lupin seeds extracts and fractions from lupinus angustifolius and lupinus albus.	the results demonstrated immunotropic activity of seeds extracts and fractions from lupinus angustifolius and lupinus albus. plaque forming cells (pfc) number to srbc (sheep red blood cells) were elevated by an extract from lupinus angustifolius and lowered by extracts from lupinus albus. all preparations obtained from lupinus angustifolius reduced the number of rosette forming cells (e-rfc). these preparations suppressed also the intensity of graft versus host reaction (gvhr) in case when the d ...	1994	7503649
genetic structure and symbiotic characteristics of a bradyrhizobium population recovered from a pasture soil.	we examined the genetic structure and symbiotic characteristics of bradyrhizobium isolates recovered from four legume species (lupinus albus [white lupine], lupinus angustifolius [blue lupine], ornithopus compressus [yellow serradella], and macroptilium atropurpureum [sirato]) grown in an oregon soil. we established that multilocus enzyme electrophoresis (mlee) can provide insights into the genetic relatedness among bradyrhizobium strains by showing a positive correlation (r = >/=0.90) between t ...	1994	16349270
effect of some environmental factors on rhizobium and bradyrhizobium strains.	effects of different abiotic factors (acidity, salinity, nitrate and temperature) on growth rate of root-nodule bacteria (rhizobium and bradyrhizobium) strains were investigated in vitro. strains isolated from vicia faba l., coronilla varia l. and lupinus albus l. exhibited a large variation in tolerance of the above-mentioned factors. these bacteria should be screened under stimulated conditions for enhanced survival before selection to be used for commercial inoculant production. linear correl ...	1995	7620814
lupinus albus l. pathogenesis-related proteins that show similarity to pr-10 proteins.	we describe a group of three acidic proteins, pathogenesis-related (pr)-p16.5a, pr-p16.5b, and pr-p16.5c, that accumulate in the leaves of lupinus albus l. cv rio maior plants when infected with the fungus colletotrichum gloeosporioides penz. these proteins co-migrate in sodium dodecyl sulfate-polyacrylamide gels as a single band of 16.5 kd, behaving as charge isomers, and are related to several members of the defense-related pr-10 protein family. localization of the proteins was investigated by ...	1995	8539294
farnesyl pyrophosphate synthase from white lupin: molecular cloning, expression, and purification of the expressed protein.	plants produce a variety of sesquiterpenoid compounds with diverse biological functions, whose synthesis is initiated by farnesyl pyrophosphate synthase [ec 2.5.1.1, ec 2.5.1.10]. the lack of availability of molecular tools to analyze this enzyme has, thus far, prevented the study of its expression and regulation in plants. a dna fragment corresponding to a portion of the farnesyl pyrophosphate synthase gene was amplified by the polymerase chain reaction using was amplified by the polymerase cha ...	1995	7646076
enrichment of an israeli ethnic food with fibres and their effects on the glycaemic and insulinaemic responses in subjects with non-insulin-dependent diabetes mellitus.	the effects of various sources of dietary fibre on the high glycaemic index of an israeli ethnic food, melawach, were investigated in subjects with non-insulin-dependent diabetes mellitus (niddm). locust-bean (ceratonia siliqua) gum significantly decreased the glucose response to, and glycaemic index of, melawach in these diabetic subjects (p < 0.05). it also tended to decrease their insulinaemic response and insulinaemic index, but differences were not significant. dietary fibre from lupin (lup ...	1995	8541274
purification and characterization of a trna nucleotidyltransferase from lupinus albus and functional complementation of a yeast mutation by corresponding cdna.	atp (ctp):trna nucleotidyltransferase (ec 2.7.7.25) was purified to apparent homogeneity from a crude extract of lupinus albus seeds. purification was accomplished using a multistep protocol including ammonium sulfate fractionation and chromatography on anion-exchange, hydroxylapatite and affinity columns. the lupin enzyme exhibited a ph optimum and salt and ion requirements that were similar to those of trna nucleotidyltransferases from other sources. oligonucleotides, based on partial amino ac ...	1996	8616252
characterisation of a developmentally related polypeptide with glutelin solubility characteristics from lupinus albus l.	proteins from lupinus albus l. cv. rio maior seeds were fractionated according to solubility criteria. patterns of concanavalin a (cona)-binding polypeptides from the different classes, albumins, globulins, glutelins and prolamins, were established by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. two bands of apparent molecular masses of 29 and 23.5 kda with glutelin solubility characteristics bound the lectin. the 23.5-kda band was separated by two-dimensional electrophoresis into ...	1996	8580776
root colonization of different plants by plant-growth-promoting rhizobium leguminosarum bv. trifolii r39 studied with monospecific polyclonal antisera.	monospecific polyclonal antisera raised against rhizobium leguminosarum bv. trifolii r39, a bacterium which was isolated originally from red clover nodules, were used to study the colonization of roots of leguminous and nonleguminous plants (pisum sativum, lupinus albus, triticúm aestivum, and zea mays) after inoculation. eight weeks after inoculation of soil-grown plants, between 0.1 and 1% of the total bacterial population in the rhizospheres of all inoculated plants were identified as r. legu ...	1997	9143133
analysis of lupin seed protein digestibility using gel electrophoresis and immunoblots.	proteins from the seeds of 12 cultivars of three lupin species were analyzed by gel electrophoresis. similarities between cultivars of the same species were noted. antibodies raised against the three major globular proteins, conglutin alpha, beta, and gamma, of lupinus albus cv. ultra were used to probe immunoblots of crude extracts. the immunoblots revealed variations between cultivars not previously resolved and identified which protein-subunits were derived from which conglutin. in vitro dige ...	1997	9222852
accumulation of a lectin-like breakdown product of beta-conglutin catabolism in cotyledons of germinating lupinus albus l. seeds.	during germination of lupinus albus seeds, a 20-kda polypeptide accumulates in the cotyledons of 4-d-old plants (ferreira et al., 1995b, j exp bot 46: 211-219). immunological, polypeptide cleavage with cyanogen bromide and amino acid sequencing experiments indicate that the 20-k-da polypeptide and ubiquitin are structurally unrelated. however, there is a strong sequence homology between the 20-kda polypeptide and the vicilin-like storage proteins from pea and soybean. our results indicate that t ...	1997	9299789
effect of sweet, bitter and soaked micronised bitter lupins on broiler performance.	1. the feeding value for broilers of sweet white lupins (lupinus albus variety hanti), bitter lupins (lupinus angustifolius) and soaked micronised bitter lupins was examined. 2. four isocaloric and isonitrogenous diets were formulated; one contained no lupins and the other 3 contained 400 g/kg sweet, bitter or soaked micronised bitter lupins. the 3 lupin diets were blended appropriately to produce 16 experimental diets containing 0, 50, 100, 200, 300 and 400 g/kg sweet, bitter and soaked microni ...	1997	9158898
iron stress and pyoverdin production by a fluorescent pseudomonad in the rhizosphere of white lupine (lupinus albus l.) and barley (hordeum vulgare l.).	induction of high-affinity iron transport during root colonization by pseudomonas fluorescens pf-5 (pvd-inaz) was examined in lupine and barley growing in microcosms. p. fluorescens pf-5 (pvd-inaz) contains a plasmid carrying pvd-inaz; thus, in this strain, ice nucleation activity is regulated by pyoverdin production. lupine or barley plants were grown for 18 or 8 days, respectively, in soil amended with 2% calcium carbonate and inoculated with p. fluorescens pf-5 (pvd-inaz) at a density of 4 x ...	1997	16535491
phloem glutamine and the regulation of o2 diffusion in legume nodules.	the aim of the present study was to test the hypothesis that the n content or the composition of the phloem sap that supplies nodulated roots may play a role in the feedback regulation of nitrogenase activity by increasing nodule resistance to o2 diffusion. treating shoots of lupin (lupinus albus cv manitoba) or soybean (glycine max l. merr. cv maple arrow) with 100 [mu]l l-1 nh3 caused a 1.3-fold (lupin) and 2.6-fold (soybean) increase in the total n content of phloem sap without altering its c ...	1997	12223605
crop rotation impacts on potato protein.	the impact of nitrogen (n) fertilization on potato (solanum tuberosum l.) protein yield and nutritional quality is well documented but of little benefit to growers with limited access to fertilizer or capital (e.g. in lesser developed countries). this study was conducted 1) to evaluate the extent which crude protein yield in potatoes can be influenced by crop rotation with no n fertilizer and 2) to determine if crop rotation and minimal application of n fertilizer can meet the total protein yiel ...	1998	10426115
effect of enzyme supplementation of uk-grown lupinus albus on growth performance in broiler chickens.		1998	10188036
genotypic and environmental effects on lupin seed composition.	currently, white lupin (lupinus albus l.) is gaining importance due to its high nitrogen fixation capability and potential in sustainable crop production systems. even though research conducted in australia, chile, germany, new zealand, and portugal has indicated lupin's positive potential as human and animal food, such information from virginia and adjoining areas of the united states is not available. in addition, effects of growing environment and genotypes on lupin seed composition need to b ...	1998	10890753
understanding the nutritional chemistry of lupin (lupinus spp.) seed to improve livestock production efficiency.	in their raw, unprocessed form, lupins have many desirable characteristics for feeding both ruminants and single-stomached animals. an emphasis on these desirable characteristics when formulating diets, combined with an advanced knowledge of how components of lupins can influence nutritional value, will ensure they make a cost-effective contribution to livestock diets. the main lupin species used in livestock diets include lupinus albus, l. angustifolius and l. luteus. supplementation of ruminan ...	1999	19087452
traditional antihelmintic, antiparasitic and repellent uses of plants in central italy.	the uses of 51 plants of marche, abruzzo and latium, distributed in 28 families, are listed here. memories and news of continued use of the plants in these sectors were collected from farmers and shepherds in person (mostly old people). the plants most frequently used as antiparasitics and repellents are juglans regia, lupinus albus, ruta graveolens, fraxinus ornus, datura stramonium, artemisia absinthium, allium cepa, while r. graveolens, cucurbita maxima, a. absinthium, allium sativum are the ...	1999	10624877
inhibition of growth and aflatoxin biosynthesis of aspergillus flavus by extracts of some egyptian plants.	the effect of five different concentrations (2, 4, 6, 8 and 10 mg ml-1) of an aqueous extracts of lupinus albus, ammi visnaga and xanthium pungens were tested on growth and aflatoxin production by aspergillus flavus in a chemically defined medium. all the plants inhibited mycelial growth and aflatoxin formation. the inhibitory effect was proportional with the applied concentration. growth and aflatoxin production appeared to be correlated processes. the nature of the plant extract also affected ...	1999	10664975
calcium- and magnesium-dependent aggregation of legume seed storage proteins.	the solubility characteristics and sedimentation behavior of total or individual globulins from legume seeds [lupinus albus l., pisum sativum l., and glycine max (l.) merr.] were investigated. the typical insolubility of globulins detected during their extraction seems to be due to the presence of a low molecular weight factor(s) in the seed extract. the solubility of the purified globulins decreases with increasing concentrations of calcium and/or magnesium, but not of other cations, showing mi ...	1999	10552601
use of a single method in the extraction of the seed storage globulins from several legume species. application to analyse structural comparisons within the major classes of globulins.	in this study, a single, improved methodology was used to extract, fractionate and purify the 11s (legumin-type or related to the alpha-conglutin from lupinus albus l.), 7s (vicilin-type or related to the beta-conglutin from l. albus) and 2s (related to the gamma-conglutin from l. albus) families of proteins from eight legume species: l. albus, glycine max (l.) merr., pisum sativum l., vicia faba l., cicer arietinum l., phaseolus vulgaris l., lens culinaris med. and arachis hypogaea l. the sedim ...	2000	11103299
characterization of isoperoxidase-b2 inactivation in etiolated lupinus albus hypocotyls.	one basic peroxidase isoenzyme, with a pi of 8.8, is present in the intercellular washing fluid in the aerial part of 6-day-old lupinus albus hypocotyl seedlings. this isoenzyme, called lup-b2, is the principal soluble component secreted into the apoplastic space and it is a constitutive enzyme along the whole length of etiolated hypocotyl. the enzymatic inactivation process which this apoplastic peroxidase undergoes is described for the first time. the kinetic constants which describe its inact ...	2000	10719177
differential expression of four genes encoding 1-aminocyclopropane-1-caroboxylate synthase in lupinus albus during germination, and in response to indole-3-acetic acid and wounding.	1-aminocyclopropane-1-carboxylate (acc) synthase (acs; ec 4.4.1.14) is the key regulatory enzyme of the ethylene biosynthetic pathway and is encoded by a multigene family in arabidopsis thaliana, tomato, mung bean and other plants. southern blot analysis revealed the existence of at least five acs genes in white lupin (lupinus albus l.) genome. four complete and one partial sequences representing different acs genes were cloned from the lupin genomic library. the levels of expression of two of t ...	2000	11089679
direct evidence for ribonucleolytic activity of a pr-10-like protein from white lupin roots.	an abundant 17 kda protein which was isolated and characterized from 10-day old healthy root tissue of white lupin (lupinus albus) proved to have a high sequence similarity to pathogenesis-related proteins found in other species. subsequently, a corresponding clone (lapr-10) was identified in a cdna library prepared from the same tissue that exhibited a high amino acid sequence similarity to a number of the pr-10 family proteins. the clone contains an open reading frame encoding a polypeptide of ...	2000	10890534
a new class of biflavonoids: 2'-hydroxygenistein dimers from the roots of white lupin.	two novel isoflavonoid dimers presumably originating from 2'-hydroxygenistein, 5,7,4'-trihydroxycoumaranochroman-4-one-(3-->5"')-5",7",2"'4"'- tetrahydroxyisoflavone (1, lupinalbisone a) and 5,7,4'-trihydroxycoumaranochroman-4-one-(3-6")-5",7",2"',4"'-te trahydroxyisoflavone (2, lupinalbisone b) were isolated from the roots of lupinus albus l., and their structures involving relative stereochemistry were elucidated by spectroscopic methods. using horse radish peroxidase and 2'-hydroxygenistein ( ...	2000	10817204
suppression of phytophthora cinnamomi in potting mixes amended with uncomposted and composted animal manures.	abstract we examined the effects of fresh and composted animal manures on the development of root rot, dieback, and plant death caused by phytophthora cinnamomi. fresh chicken manure, or chicken manure composted for 5 weeks before incorporation into the potting mix (25%, vol/vol), significantly reduced pathogen survival and the development of symptoms on lupinus albus seedlings. chicken manure composted for 2 weeks was less suppressive. cow, sheep, and horse manure, whether fresh or composted, d ...	2000	18944498
the lupinus albus class-iii chitinase gene, if3, is constitutively expressed in vegetative organs and developing seeds.	a cdna fragment encoding a lupinus albus. l. class-iii chitinase, if3, was isolated, using a cdna probe from cucumis sativus l., by in-situ plaque hybridization from a cdna library constructed in the uni-zap xr vector, with mrnas isolated from mature lupin leaves. the cdna had a coding sequence of 293 amino acids including a 27-residue n-terminal signal peptide. a class-iii chitinase gene was detected by southern analysis in the l. albus genome. western blotting experiments showed that the if3 p ...	2000	10787047
effect of food enzymes on utilisation of lupin carbohydrates by broilers.	1. the effects of 3 commercial enzyme products on the nutritive value of 2 lupin species were investigated with the emphasis on changes in composition of non-starch polysaccharides (nsps) along the digestive tract. enzyme a contained primarily cellulase, beta-glucanase and xylanase activities, enzyme b primarily hemicellulase, pentosanase and xylanase activities, and enzyme c primarily hemicellulase, pectinase and beta-glucanase activities. 2. the enzymes were added to semi-purified diets based ...	2000	10821527
characterization and expression analysis of the yellow lupin (lupinus luteus l.) gene coding for nodule specific proline-rich protein.	the llprp2 gene coding for a proline-rich protein shows a high level of similarity to, as well as significant differences from the family of enod2 nodule-specific genes. several sequence motifs with putative regulatory function were identified in the 5' and 3' noncoding regions of the llprp2 gene. northern blot analysis revealed that the expression of the llprp2 gene begins 9 days after inoculation of yellow lupin roots with bradyrhizobium sp. (lupinus); the expression is restricted to symbiotic ...	2000	11051202
variability in plant-microbe interaction between lupinus lines and bradyrhizobium strains.	even though lupin (lupinus albus l.) is known to potentially fix 150-200 kg/ha nitrogen for the use of a succeeding crop, precise information about lupinxbradyrhizobium strain interaction under the climatic conditions prevalent in the mid-atlantic region of the united states is unknown. we conducted two greenhouse experiments with the objective of characterizing this symbiotic relationship and to evaluate potential interaction between bradyrhizobium strains and lupin lines. in the first experime ...	2000	11074278
[risk of serious acute asthma due to lupine flour associated with peanut allergy].	lupine flour (lupinus albus), recently authorized in france in human food, cross-reacts with peanuts. we report a case of acute asthma in a patient with allergy to peanuts. exegesis: this patient has a severe allergy to peanuts, presenting as acute asthma. skin prick-tests to raw and cooked lupine flour were positive. the level of specific-ige (allerbio, france) to lupine flour were high. oral challenge test induced acute asthma at a dose of 965 mg of lupine flour. this quantity may be included ...	2000	10703077
profiling changes in metabolism of isoflavonoids and their conjugates in lupinus albus treated with biotic elicitor.	liquid chromatography with ultraviolet and mass spectrometric detection was applied to monitor changes in profiles of isoflavonoid glycosides and free isoflavonoid aglycones in lupinus albus l. four isoflavonoid aglycones, fourteen isoflavonoid glycosides, four flavonol glycosides and flavone glycoside were identified in lupin tissue after lc/esi/ms analyses. an elicitor preparation from purified yeast cell wall was used to inject the shoots of 3-week old seedlings or to infiltrate the cut lupin ...	2001	11198822
pathogenic infection and the oxidative defences in plant apoplast.	the structural and functional continuum of the plant apoplast is the first site of contact with a pathogen and plays a crucial role in initiation and coordination of many defence responses. in this paper, we present an overview of the involvement of the plant apoplast in plant-pathogen interactions. the process of infection of french bean (phaseolus vulgaris l.) plants by colletotrichum lindemuthianum is analysed. the ultrastructural features of plant defence responses to fungal infection are th ...	2001	11732333
short- and long-term effects of dehydroascorbate in lupinus albus and allium cepa roots.	administration of 1 mm dehydroascorbate (dha) results in a rapid and large increase in cellular ascorbate (aa) content in both lupinus albus l. and allium cepa l. root tips. uptake of dha from the medium occurs at a high rate within 10-12 h of incubation, and is slowed down thereafter. in the first few h, dha reduction to aa is apparently correlated to gsh depletion and slightly higher dha reductase activity. dha incubation also seems to induce new gsh synthesis. longer dha incubation (24 h) aff ...	2001	11522912
identification and characterisation of the ige-binding proteins 2s albumin and conglutin gamma in almond (prunus dulcis) seeds.	almond proteins can cause severe anaphylactic reactions in susceptible individuals. the aim of this study was the identification of ige-binding proteins in almonds and the characterisation of these proteins by n-terminal sequencing.	2002	12065909
biochemical study on the effects of some egyptian herbs in alloxan-induced diabetic rats.	the present study was carried out to investigate the effects of lupinus albus, l. (lupinus termis), family l. leguminosae, cymbopogon proximus, (halfa barr), family gramineae, and zygophyllum coccineum l. (kammun quaramany), family l. zygophyllacae on biochemical parameters in alloxan-induced diabetic rats. a dose of 1.5 ml of aqueous suspension of each herb/100 g body weight (equivalent to 75 mg/100 g b.wt.) was orally administered daily to alloxan-diabetic rats for 4 weeks. the levels of gluco ...	2002	11788159
effect of some hypoglycemic herbs on the activity of phase i and ii drug-metabolizing enzymes in alloxan-induced diabetic rats.	polycyclic aromatic hydrocarbons (pahs) and n-nitrosamines (nna) are mainly activated by cytochrome p450s, and their associated enzyme activities such as aryl hydrocarbon (benzo(a)pyrene) hydroxylase (ahh), n-nitrosdimethylamine n-demethylase i (ndma-di), nadph-cytochrome c reductase, and detoxified by glutathione s-transferase (gst) and glutathione (gsh). the present study shows the influence of cymbopogon proximus (halfa barr), zygophyllum coccineum l. (kammun quaramany), lupinus albus (termis ...	2002	11985890
the bioavailability and postprandial utilisation of sweet lupin (lupinus albus)-flour protein is similar to that of purified soyabean protein in human subjects: a study using intrinsically 15n-labelled proteins.	sweet lupin (lupinus albus), a protein-rich legume devoid of anti-nutritional factors, is considered to have a high potential for protein nutrition in man. results concerning the nutritional value of lupin protein are, however, conflicting in animals and very scarce in human subjects. furthermore, where fibre-rich protein sources are concerned, the long-term nutritional results are often obscured, particularly since fibre-promoted colonic fermentation may bias the energy supply and redistribute ...	2002	12064341
the effect of nitrogen nutrition on cluster root formation and proton extrusion by lupinus albus.	nitrogen nutrition can influence cluster root formation in many wild species, but the effect of n form on cluster root formation and root exudation by white lupin is not known. in a solution culture study, we examined the effect of n nutrition (ammonium, nitrate, both or n2 fixation) on cluster root formation and h+ extrusion by white lupin plants under deficient and adequate p supply. the number of cluster roots increased greatly when plants were supplied with i microm p compared with 50 microm ...	2002	12096804
purification and characterization of three phytases from germinated lupine seeds (lupinus albus var. amiga).	three phytases were purified about 14200-fold (lp11), 16000-fold (lp12), and 13100-fold (lp2) from germinated 4-day-old lupine seedlings to apparent homogeneity with recoveries of 13% (lp11), 8% (lp12), and 9% (lp2) referred to the phytase activity in the crude extract. they behave as monomeric proteins of a molecular mass of about 57 kda (lp11 and lp12) and 64 kda (lp2), respectively. the purified proteins belong to the acid phytases. they exhibit a single ph optimum at 5.0. optimal temperature ...	2002	12405788
effects of a vegetable extract from lupinus albus (lu105) on the production of matrix metalloproteinases (mmp1, mmp2, mmp9) and tissue inhibitor of metalloproteinases (timp1, timp2) by human gingival fibroblasts in culture.	this study examined the effects of a vegetable extract from lupinus albus (lu105) on mmps and timps secreted by human gingival fibroblasts in culture. lu105 was extracted from seeds of l. albus and is freely soluble in water. gelatin zymography showed that control human gingival fibroblasts maintained in culture for 48 h express pro-mmp2 (progelatinase a) in the culture medium while the active form of mmp2 (gelatinase a), the active form of mmp9 (gelatinase b), and pro-mmp9 (progelatinase b) are ...	2003	12802622
profiling of flavonoid conjugates in lupinus albus and lupinus angustifolius responding to biotic and abiotic stimuli.	qualitative and quantitative composition of flavonoid and isoflavonoid glycosides as well as free aglycones in lupin seedlings (roots and aerial parts) grown under different light conditions or responding to infection with pleiochaeta setosa, a fungus causing brown leaf spot, were monitored by liquid chromatography with uv and/or mass spectrometric detection. both physical and biotic factors affected flavonoid and isoflavonoid levels in lupin tissues. fungal infection evoked significant increase ...	2003	12857026
protective effect of a vegetable extract from lupinus albus (lu 105) on human gingival elastic fibers degradation by human leukocyte elastase.	the aim of this study was to determine if a vegetable extract from seeds of lupinus albus (lu 105) has the capacity to inhibit human leukocyte elastase and/or protect gingival elastic fibers against proteolytic degradation. lu105 was extracted from seeds of l albus and is freely soluble in water. in this study the ex-vivo elastolytic activity of human leukocyte elastase and the potential inhibitory effect of lu 105 were determined using human gingival cryostat tissue sections and computerized mo ...	2003	14508666
accumulating behaviour of lupinus albus l. growing in a normal and a decalcified calcic luvisol polluted with zn.	lupinus albus l. is a leguminous plant that is starting to generate interest for the phytoremediation soils showing intermediate metal pollution. among these metals, zn causes major phytotoxicity problems and is common in polluted soils of central spain. the purpose of this study was to explore the nutritional behaviour of this plant species towards increasing zn concentrations in two calcic luvisol soils: a normal basic soil and a decalcified acid soil. for this purpose the effects of different ...	2003	14717438
chemical composition and antibacterial activities of lupin seeds extracts.	determination of influence of lupin natural phenolic compounds on antibacterial properties of its seeds was carried out. raw material were seeds of lupinus albus, l. luteus, and l. angustifolius. the methods included the determination of the content of proteins, total phenolic compounds, free phenolic acids, and tannins as well as antibacterial properties with ethanol extracts. the content of total phenolic compounds was smaller in testas than in cotyledons and the highest levels are observed in ...	2003	14609081
a biochemical and molecular characterization of lep1, an extensin peroxidase from lupin.	an analysis of apoplastic extensin cross-linking activity in vegetative organs of lupinus albus indicated that leaves contained the highest specific activity. assays of peroxidases fractionated from this material demonstrated that this activity could be largely attributed to a soluble and apoplastic 51-kda peroxidase, denoted lep1. relative to other purified peroxidases, lep1 demonstrates high extensin cross-linking activity and can be classified as an extensin peroxidase (ep). optimal condition ...	2003	12882982
lupin nad9 and nad6 genes and their expression: 5' termini of the nad9 gene transcripts differentiate lupin species.	the mitochondrial nad9 and nad6 genes were analyzed in four lupin species: lupinus luteus, lupinus angustifolius, lupinus albus and lupinus mutabilis. the nucleotide sequence of these genes confirmed their high conservation, however, higher number of nucleotide substitution was observed in the l. albus genes. southern hybridizations confirmed the presence of single copy number of these genes in l. luteus, l. albus and l. angustifolius. the expression of nad9 and nad6 genes was analyzed by northe ...	2003	14557072
a systemic small rna signaling system in plants.	systemic translocation of rna exerts non-cell-autonomous control over plant development and defense. long-distance delivery of mrna has been proven, but transport of small interfering rna and microrna remains to be demonstrated. analyses performed on phloem sap collected from a range of plants identified populations of small rna species. the dynamic nature of this population was reflected in its response to growth conditions and viral infection. the authenticity of these phloem small rna molecul ...	2004	15258266
establishment of selenium uptake and species distribution in lupine, indian mustard, and sunflower plants.	selenium has been recognized as essential for all mammals; therefore, its concentration level and speciation are of great concern. plants are one of the main sources of selenium in the diet. thus, inorganic selenium uptake and its transformation in different species were evaluated in indian mustard (brassica juncea), sunflower (helianthus annus), and white lupine (lupinus albus). more than 1.2 g x kg(-)(1) (dry matter) of se was found in the aerial part of indian mustard when growing on 1 mg x l ...	2004	14969538
utilization of the chicken embryo model for assessment of biological activity of different oligosaccharides.	the effect of different oligosaccharides--alpha-galactoside preparations from lupinus albus seeds differing in sucrose content, raffinose and fructooligosaccharides on the growth of chicken intestine microflora and the hatchability and weight of the treated embryos were studied. the assessment of biological activity of these oligosaccharides was done in ovo on the chicken embryo model. the eggs of experimental groups containing twelve days old embryos were injected into the air cell with 0.2 ml ...	2004	19058551
nutrient supply to dairy cows from processed white lupines.	the objective of this study was to compare the dve/oeb system (dve = truly absorbed protein in the small intestine; oeb = degraded protein balance (dpb) in dutch) and the nrc-2001 model in the prediction of supply of protein to dairy cows from processed white lupines (lupinus albus l.). comparisons were made in terms of (1) ruminally synthesized microbial cp, (2) truly absorbed protein in the small intestine, and (3) degraded protein balance. in addition, the systematic investigation of roasting ...	2004	15195906
proteins of white lupin seed, a naturally isoflavone-poor legume, reduce cholesterolemia in rats and increase ldl receptor activity in hepg2 cells.	white lupin (lupinus albus, l.), a widely cultivated crop that has been consumed for many years in western europe, may provide a useful alternative for individuals wishing to substitute animal with plant proteins for cardiovascular disease prevention. lupin seeds have a very low content of isoflavones, and lupin protein isolates are essentially isoflavone free. in rats fed a casein-based cholesterol + cholic acid diet, a relatively low daily intake (50 mg/d by gavage for 2 wk) of total lupin pro ...	2004	14704287
lupinus albus, a novel vegetable extract with metalloproteinase inhibitory properties: a potential periodontal therapy.	in this study we examine the properties of a vegetable extract from seeds of lupinus albus (lu 105). in previous works we demonstrated that lu 105 reduced the expression, by gingival fibroblasts, of both matrix metalloproteinase (mmp)-2 and mmp-9. we decided to study the impact of lu 105 on cell proliferation and morphology. using organ culture media we also studied the mmp and tissue inhibitors of metalloproteinases (timp) expression and the cytokines secretion.	2005	16101366
ethnobotanical and ethnomedicinal uses of plants in the district of acquapendente (latium, central italy).	in the years 2002-2003 research was carried out concerning ethnomedicine in the acquapendente district (viterbo, latium, central italy), an area so far less frequently studied from the perspective of plant folk traditions. the district, from the ethnobotanical point of view, shows traces of the influences of the neighbouring regions. in this study 96 plant entities are described, belonging to 45 families, of which 64 are employed in human medicine, 15 in veterinary medicine, 22 in the feeding of ...	2005	15619562
traditional phytotherapy in central italy (marche, abruzzo, and latium).	in this study, the more significant results of extensive ethnopharmacobotanical research carried out by the author in the years 1977-2000 in 175 localities of three regions of central italy (marche, abruzzo, and latium) have been reported and compared. the usages of 80 species belonging to 36 families are described, of which 71 were used in human therapy and 29 in veterinary medicine. uses are suited with the number of localities in which they have been mentioned. among the wild plant mainly sti ...	2005	15664457
plant growth promotion abilities and microscale bacterial dynamics in the rhizosphere of lupin analysed by phytate utilization ability.	in the rhizosphere, phosphorus (p) levels are low because of p uptake into the roots. rhizobacteria live on carbon (c) exuded from roots, and may contribute to plant nutrition by liberating p from organic compounds such as phytates. we isolated over 300 phytate (na-inositol hexa-phosphate; na-ihp)-utilizing bacterial strains from the rhizosheath and the rhizoplane of lupinus albus (l.). almost all of the isolates were classified as burkholderia based on 16s rdna sequence analysis. rhizosheath is ...	2005	15683400
nodulation of lupinus albus by strains of ochrobactrum lupini sp. nov.	the nodulation of legumes has for more than a century been considered an exclusive capacity of a group of microorganisms commonly known as rhizobia and belonging to the alpha-proteobacteria. however, in the last 3 years four nonrhizobial species, belonging to alpha and beta subclasses of the proteobacteria, have been described as legume-nodulating bacteria. in the present study, two fast-growing strains, lup21 and lup23, were isolated from nodules of lupinus honoratus. the phylogenetic analysis ...	2005	15746334
raffinose family of oligosaccharides from lupin seeds as prebiotics: application in dairy products.	the raffinose family of oligosaccharides (rfos) isolated from lupin seeds (lupinus albus var. multolupa) was evaluated for bifidogenic effects during the manufacture of probiotic fermented milk. a mixed starter inoculum was composed of bifidobacterium lactis bb-12 and lactobacillus acidophilus (1:1). lupins are a rich source of rfos that can be used as functional food ingredients. the addition of rfos to milk increased b. lactis bb-12 and l. acidophilus populations at the final fermentation time ...	2005	15954717
characterization of bacterial community structure in rhizosphere soil of grain legumes.	molecular techniques were used to characterize bacterial community structure, diversity (16s rdna), and activity (16s rrna) in rhizospheres of three grain legumes: faba beans (vicia faba l., cv. scirocco), peas (pisum sativum l., cv. duel) and white lupin (lupinus albus l., cv. amiga). all plants were grown in the same soil under controlled conditions in a greenhouse and sampled after fruiting. amplified 16s rdna and rrna products (using universal bacterial primers) were resolved by denaturing g ...	2005	16003473
phyllobacterium trifolii sp. nov., nodulating trifolium and lupinus in spanish soils.	bacterial strain petp02(t) was isolated from nodules of trifolium pratense growing in a spanish soil. phylogenetic analysis of the 16s rrna gene sequence showed that this strain represents a member of the genus phyllobacterium. however, divergence found with the 16s rrna gene sequence of the single recognized species of this genus, phyllobacterium myrsinacearum, indicated that strain petp02(t) belongs to a different species. the results of dna-dna hybridization, phenotypic tests and fatty acid a ...	2005	16166699
transgenic proteoid roots of white lupin: a vehicle for characterizing and silencing root genes involved in adaptation to p stress.	white lupin (lupinus albus l.) has become an illuminating model for the study of plant adaptation to phosphorus (p) deficiency. it adapts to -p stress with a highly coordinated modification of root development and biochemistry resulting in short, densely clustered secondary roots called proteoid (or cluster) roots. in order to characterize genes involved in proteoid root formation and function in a homologous system, we have developed an agrobacterium rhizogenes-based transformation system for w ...	2005	16297074
alterations induced by glyphosate on lupin photosynthetic apparatus and nodule ultrastructure and some oxygen diffusion related proteins.	the effects of glyphosate on protein metabolism, mesophyll cell ultrastructure and nodule ultrastructure and functioning of lupinus albus cv. multolupa inoculated with bradyrhizobium sp. (lupinus) were investigated. young leaves and nodules were especially affected because these organs act as sinks of the herbicide. the alterations on nodular and chloroplast ultrastructure varied depending on herbicide concentration and time of exposure. after 3 days of 2.5 mm glyphosate application some toxic e ...	2005	16324849