Publications

overview of proposed mechanisms for the hypocholesterolemic effect of soy.	a large body of literature indicates that protein from soybeans reduces blood cholesterol concentrations in experimental animals as well as in humans. the mechanism and component of soy responsible has not been established fully. some suggest that when soy protein is fed, cholesterol absorption and/or bile acid reabsorption is impaired. this is observed in some animal species, such as rabbits and rats, but not in humans nor when amino acids replace intact soy protein. others propose that changes ...	1995	7884541
saponins as anticarcinogens.	saponins, which are present in plants, have been suggested as possible anticarcinogens. they possess surface-active characteristics that are due to the amphiphilic nature of their chemical structure. the proposed mechanisms of anticarcinogenic properties of saponins include direct cytotoxicity, immune-modulatory effects, bile acid binding and normalization of carcinogen-induced cell proliferation. however, the anticarcinogenic effects of saponins from commonly consumed plant foods have not been ...	1995	7884557
the evidence for soybean products as cancer preventive agents.	there is much evidence suggesting that compounds present in soybeans can prevent cancer in many different organ systems. the evidence for specific soybean-derived compounds having a suppressive effect on carcinogenesis in animal model systems is limited, however. there is evidence that the following isolated soybean derived products suppress carcinogenesis in vivo: a protease inhibitor, the bowman-birk inhibitor, inositol hexaphosphate (phytic acid) and the sterol beta-sitosterol. other compound ...	1995	7884559
possible adverse effects of soybean anticarcinogens.	for soybeans to serve as a good source of protein for feeding animals as well as humans, a certain amount of heat treatment or some other form of processing must be applied. this is because there are present in soybeans certain heat-labile factors that exert an adverse effect on the nutritional value of the protein. the so-called protease inhibitors have received the most attention in this regard and have been shown to exert their antinutritional effect in the short term by causing pancreatic hy ...	1995	7884560
in vitro hormonal effects of soybean isoflavones.	isoflavones exhibit a multitude of biological effects that influence cell growth and regulation, and, thus, may have potential value in the prevention and treatment of cancer. isoflavones are weak estrogens and can function both as estrogen agonists and antagonists depending on the hormonal milieu and the target tissue and species under investigation. genistein, one of the two primary isoflavones in soybeans, has attracted much attention from the research community, not only because of its poten ...	1995	7884561
antioxidant and antipromotional effects of the soybean isoflavone genistein.	antioxidant and antipromotional effects of the soybean isoflavone genistein have been studied in hl-60 cells and the mouse skin tumorigenesis model. effects of structure-related flavone/isoflavones on hydrogen peroxide (h2o2) production by 12-o-tetradecanoylphorbol-13-acetate (tpa)-activated hl-60 cells and superoxide anion (o2-) generation by xanthine/xanthine oxidase were compared. of tested isoflavones, genistein is the most potent inhibitor among tpa-induced h2o2 formation by (dimethyl sulfo ...	1995	7892286
control of de novo purine biosynthesis genes in ureide-producing legumes: induction of glutamine phosphoribosylpyrophosphate amidotransferase gene and characterization of its cdna from soybean and vigna.	soybean (glycine max) and mothbean (vigna aconitifolia) cdna clones encoding glutamine phosphoribosylpyrophosphate amidotransferase (prat), the first enzyme of the de novo purine biosynthesis pathway, have been isolated from nodule cdna libraries. the amino acid sequence deduced from soybean clone showed > 85% homology to the prat sequence of mothbean and 33-47% homology to those of bacteria, yeast, chicken, rat and human. the soybean clone encodes a protein with an n-terminal sequence resemblin ...	1995	7894513
high-performance liquid chromatographic assay of isoflavonoids and coumestrol from human urine.	a rapid, sensitive and precise diode-array reversed-phase hplc method was developed for human urine analysis of the most common dietary isoflavones daidzein, genistein, formononetin and biochanin-a, their mammalian metabolites equol and o-desmethylangolensin, and of coumestrol, another commonly occurring phytoestrogen. analytes were isolated and concentrated by solid-phase extraction and separated by hplc followed by identification through retention times and uv scans, and in the case of coumest ...	1994	7894693
identification of a homeobox-containing gene with enhanced expression during soybean (glycine max l.) somatic embryo development.	homeotic gene are key 'switches' that control developmental processes. homeotic genes containing the consensus 'homeobox' domain have been identified from a number of organisms including drosophila melanogaster, caenorhabditis elegans, homo sapiens, and zea mays. although homeotic genes have been demonstrated to be important in embryo development of some insects, amphibians, and mammals, there are no reports of their involvement in plant embryogenesis. here, we report the isolation and character ...	1994	7907232
immunohistochemical investigations on the pyloric glands of the ruin lizard (podarcis sicula campestris de betta).	mucous cells and enteroendocrine cells of the pyloric region of the ruin lizard (podarcis sicula campestris de betta) have been examined by lectin histochemical and immunohistochemical methods. binding to five plant lectins (canavalia ensiformis, con a; triticum vulgare, wheat germ, wgl; lotus tetragonolobus, winged pea, wpl; glycine max, soybean, sbl; arachis hypogaea, peanut, pnl) was performed to characterize glycoconjugates in the secretory products of superficial and glandular mucous cells. ...	1994	7913280
structures of nodulation factors from the nitrogen-fixing soybean symbiont rhizobium fredii usda257.	we have isolated and characterized the extracellular nod factors of rhizobium fredii usda257, a nitrogen-fixing symbiont of soybean [glycine max (l.) merr.] and several other legume species. these signals are produced upon exposure to the isoflavone genistein and consist of a series of substituted, beta 1,4-linked tri-, tetra-, and pentamers of n-acetylglucosamine. n-vaccenic acid replaces acetate on the nonreducing residue, and the reducing residue contains alpha-linked 2-o-methylfucose on carb ...	1994	7918395
structure of the pisum sativum seed lipoxygenase gene lox1:ps:3.	we have isolated the pisum sativum (pea) lox1:ps:3 gene which encodes a lipoxygenase that is 84% identical in coding sequence to the lox-3 gene from glycine max (soybean). the 10028 bp sequence includes 5895 bp 5' to the transcription start site and 707 bp 3' to the stop codon. the coding region of lox1:ps:3 contains eight introns, at positions equivalent to those in the soybean lox-3 and l-4, the phaseolus vulgaris (frenchbean) lox1, and the pisum sativum lox1:ps:2 genes. the first intron is un ...	1994	7918618
identification of peanut agglutinin and soybean trypsin inhibitor as minor legume allergens.	peanuts and soybeans are frequent causes of food hypersensitivity reactions in children. sera from 12 patients with atopic dermatitis and a positive double-blind placebo-controlled food challenge to peanut and sera from 5 patients with atopic dermatitis and a positive double-blind placebo-controlled food challenge to soybean were used to identify and characterize specific legume allergens. identification of a minor allergen from peanut and a minor allergen from soybean was accomplished using var ...	1994	7920014
genetic length polymorphisms create size variation in proline-rich proteins of the cell wall.	two genes, prp1 and prp2, encode proline-rich proteins that are found in different stages of developing seed coats, hypocotyls, and roots of soybeans (glycine max (l.) merr.). prp1 is found in young seed coats and prp2 is found later during seed desiccation. in some soybean varieties, both proteins are smaller as determined by immunoblotting seed coat proteins separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis. it was found that prp1 and prp2 genes are linked to each other by ...	1994	7920710
novel isoprenylated proteins identified by an expression library screen.	isoprenylated proteins are involved in eukaryotic cell growth and signal transduction. the protein determinant for prenylation is a short carboxyl-terminal motif containing a cysteine, to which the isoprenoid is covalently attached via thioether linkage. to date, isoprenylated proteins have almost all been identified by demonstrating the attachment of an isoprenoid to previously known proteins. thus, many isoprenylated proteins probably remain undiscovered. to identify novel isoprenylated protei ...	1994	7929216
a method of purifying feline t lymphocytes from peripheral blood using the plant lectin from pisum sativum.	the binding properties of several plant lectins on feline peripheral blood lymphocytes (pbl) were examined by flow cytometry to reveal if any could serve as probes for identifying specific lymphocyte subclasses. most of the lectins bound > or = 90% of pbl in a uniform manner. the exceptions were the lectins from glycine max, phaseolus limensis, and dolichos biflorus, which bound only a proportion of the pbl. the differential binding of the lectins to lymphocyte subclasses was examined by compari ...	1994	7930648
abnormal glycosylation of motor neurons with n-acetyl-d-galactosamine in a case of subacute motor neuronopathy associated with lymphoma.	lectins, glycine max (sba) and vicia villosa (vva), which identify terminal n-acetyl-d-galactosamine (galnac), stained the motor neurons of a patient with subacute motor neuronopathy associated with neoplastic angioendotheliosis. in normal controls and in patients with other neurological disorders including amyotrophic lateral sclerosis, neither lectin stained motor neurons. abnormal glycosylation of motor neurons with terminal galnac may be associated with the pathogenesis of subacute motor neu ...	1994	7931431
histochemical study by lectin binding of surface glycoconjugates in the developing olfactory system of rat.	lectin-binding histochemistry was used to investigate the distribution and density of defined carbohydrate sequences on the cell surface glycoproteins of the olfactory receptors of rat during development. the olfactory and vomeronasal receptors showed a positive labelling after biotinylated lycopersicum esculentum lectin binding on embryonic day 16 (e16), while horseradish peroxidase-labelled glycine max, bandeiraea simplicifolia (bsa-i) and its b4 isomer bsa-i-b4 agglutinins started to label fr ...	1994	7942093
in situ characterization of the human hyalocyte.	to provide more definitive evidence regarding the cellular origin of hyalocytes and to establish a basis for their identification in tissue specimens.	1994	7945040
lipoxygenase activity associated to isolated soybean plasma membranes.	highly purified soybean (glycine max l. merr.) plasma membranes exhibit a lipoxygenase activity with a ph optimum in the acidic (5.5-6.0) range and with a km value of 200 microm for both linolenic and linoleic acids. this activity is inhibited by nordihydroguaiaretic acid (ndga), salicylhydroxamic acid (sham) and propyl gallate, stimulated by cacl2 up to 0.25 mm, h2o2 (5 to 10 nm range) and by some nucleotide triphosphates (125 to 1000 nm range) in the following order atp > gtp = utp > ctp. the ...	1994	7947991
inheritance of polymorphic markers generated by dna amplification fingerprinting and their use as genetic markers in soybean.	dna amplification fingerprinting (daf) using a high primer-to-template ratio and single, very short arbitrary primers, was used to generate amplified fragment length polymorphic markers (aflp) in soybean (glycine max (l.) merr.). the inheritance of aflps was studied using a cross between the ancestral glycine soja pi468.397 and glycine max (l.) merr. line nts382, f1 and f2 progeny. the amplification reaction was carried out with soybean genomic dna and 8 base long oligonucleotide primers. silver ...	1994	7948860
molecular analysis of two ypt/rab-related sequences isolated from soybean (glycine max) dna libraries.	from nodule and seedling cdna libraries we isolated cdna copies of two mrnas, derived from the genes gmr1 and gmr2, encoding members of the ypt/rab family of small gtp-binding proteins. two deduced protein products, gmr1 and gmr2, were found to be nearly identical differing by only four amino acids in the analysed parts. the two putative proteins are 79% identical to the previously described ara small gtpase from arabidopsis thaliana. the gmr proteins may thus be the counterpart of the ara prote ...	1994	7948868
the distribution of n-acetylgalactosamine in the cochlear nucleus of the gerbil revealed by lectin binding with soybean agglutinin.	a horseradish peroxidase conjugated lectin from glycine max (soy bean agglutinin; sba) was used to characterise the distribution of n-acetylgalactosamine in the cochlear nucleus of the mongolian gerbil. sba bound differentially to a variety of structures within the cochlear nucleus. specific sba labelling was associated with large non-granule neurones of variable size and shape throughout the cochlear nucleus. compared to adjacent nissl-stained sections 80% of the non-granule cells in the dorsal ...	1994	7961177
soybeans show promise in cancer prevention.		1994	7966389
hypomethylated sequences: characterization of the duplicate soybean genome.	soybean is believed to be a diploidized tetraploid generated from an allotetraploid ancestor. in this study, we used hypomethylated genomic dna as a source of probes to investigate the genomic structure and methylation patterns of duplicated sequences. forty-five genomic clones from phaseolus vulgaris and 664 genomic clones from glycine max were used to examine the duplicated regions in the soybean genome. southern analysis of genomic dna using probes from both sources revealed that greater than ...	1994	7969033
high-performance capillary electrophoresis for characterization of hapten-protein conjugates used for production of antibodies against soyasaponin i.	micellar electrokinetic capillary chromatography using sodium cholate as the micellar phase has been investigated for characterization of hapten-protein conjugates. special focus has been placed on the hapten soyasaponin i which is a quantitatively dominating glycoside in seeds of several legumes including pea (pisum sativum l.) and soybean [glycine max (l.) merr.]. soyasaponin i has been isolated from pea and used as hapten for production of anti-saponin specific polyclonal antibodies. soyasapo ...	1994	7981825
recovery of amino acids at the distal ileum for determining apparent and true ileal amino acid digestibilities in growing pigs fed various heat-processed full-fat soybean products.	ten crossbred gilts fitted with simple t-cannulas at the distal ileum were used to determine the apparent and true ileal amino acid digestibilities in five different soybean products: extruded, jetsploded, micronized, or roasted full-fat soybeans (ffsb) and soybean meal (sbm). the gilts with an average initial body weight of 36 kg were fed the different diets according to a replicated 5 x 5 latin square design. gilts were fed twice daily at 0800 and 1830 at 2.6 times maintenance energy requireme ...	1994	7982832
nod factors of rhizobium are a key to the legume door.	symbiotic interactions between rhizobia and legumes are largely controlled by reciprocal signal exchange. legume roots excrete flavonoids which induce rhizobial nodulation genes to synthesize and excrete lipo-oligosaccharide nod factors. in turn, nod factors provoke deformation of the root hairs and nodule primordium formation. normally, rhizobia enter roots through infection threads in markedly curled root hairs. if nod factors are responsible for symbiosis-specific root hair deformation, they ...	1994	7984092
seed-specific gene activation mediated by the cre/lox site-specific recombination system.	the cre/lox site-specific recombination system was used to activate a transgene in a tissue-specific manner. cre-mediated activation of a beta-glucuronidase marker gene, by removal of a lox-bounded blocking fragment, allowed the visualization of the activation process. by using seed-specific promoters, the timing and efficiency of gene activation could be followed within the developing tobacco (nicotiana tabacum) embryo. to serve as a basis for analyzing gene expression after-cre-mediated activa ...	1994	7991679
experimental evidence of a plant meridian system: ii. the effects of needle acupuncture on the temperature changes of soybean (glycine max).	when stainless steel needles were inserted opposite each other in the stem near the unifoliolate buds of soybean plants and retained there for the whole experiment, leaf temperatures increased for two consecutive days. following acupuncture, the mean temperature of the main vein in the first and second days increased 0.59 degree c and 0.48 degree c, respectively, while the temperature of mesophyll increased only 0.50 degree c and 0.47 degree c in the first and second days. these results are very ...	1994	7992809
position 713 is critical for catalysis but not iron binding in soybean lipoxygenase 3.	the role of asparagine-713 in iron atom incorporation and catalysis in soybean lipoxygenase 3 was investigated using site-directed mutagenesis. a full-length cdna for the lipoxygenase isoenzyme was obtained from a library derived from soybeans cv. provar. protein with native specific activity at ph 7.4 was obtained from expression in escherichia coli. two recent structure reports provided conflicting views about the participation of the side chain of asparagine-694 in the coordination of the iro ...	1994	7999759
cloning and expression of the soybean dapa gene encoding dihydrodipicolinate synthase.	the rate-limiting step in the pathway for lysine synthesis in plants is catalyzed by the enzyme dihydrodipicolinate synthase (ds). we have cloned the portion of the soybean (glycine max cv. century) dapa cdna that encodes the mature ds protein. expression of the cloned soybean cdna, as a lacz fusion protein was selected in a dapa- escherichia coli auxotroph. the ds activity of the fusion protein was characterized in e. coli extracts. the ds activity of the fusion protein was inhibited by lysine ...	1994	8000011
genetic mapping and variability of seven soybean simple sequence repeat loci.	microsatellites or simple sequence repeats are stretches of short tandemly repeated dna sequence motifs, dispersed throughout the genomes of most eukaryotes. simple sequence repeat polymorphisms (ssrps) have recently been reported in plants. here we present the genetic map position of seven different soybean (glycine max (l.) merr. and glycine soja sieb. and zucc.) ssrps contained in sequenced genes, four of which represent newly mapped positions for these genes. the other three ssrps coincided ...	1994	8001811
[improvement of protein quality of popped sorghum with soybean grain].	the present study was carried out to evaluate the chemical composition and protein quality of popped sorghum by thermic dry processing. the chemical results indicated losses in the ether extract and increases in crude fiber. likewise, losses were found in the essential amino acids, lysine and tryptophan, of 26 and 45%, respectively. the loss in these amino acids was confirmed by biological studies in which the protein quality of the raw sorghum, based on a per was 32% of the casein value and of ...	1993	8002702
identification and characterization of the nolyz genes of bradyrhizobium japonicum.	characterization of an isoflavone-inducible locus closely linked to the common nod genes of bradyrhizobium japonicum usda110 led to the discovery of two open reading frames, designated noly and nolz. these open reading frames are preceded by a sequence with strong similarity to a consensus nodd-binding site (nod box). studies utilizing a nolz'-'lacz fusion indicated that inducible expression is dependent upon both nodd1 and nodw, transcriptional regulators that are required for the expression of ...	1994	8012039
packed-column gas chromatographic method for the simultaneous determination of 10 pyrethroid insecticide residues in fruits, vegetables, and grains.	a simple, rapid, packed-column gas chromatographic method was developed for simultaneous determination of 10 pyrethroid insecticide residues (allethrin, biphenthrin, cyhalothrin, permethrin, cyfluthrin, flucythrinate, fenvalerate, fluvalinate, deltamethrin, and py-115) in fruits, vegetables, and grains. these multiresidues are extracted from various crops with acetone-petroleum ether and cleaned up on a florisil column (for the fruits and vegetables) or on a florisil-charcoal-alumina column (for ...	2015	8012228
molecular basis of a null mutation in soybean lipoxygenase 2: substitution of glutamine for an iron-ligand histidine.	we have investigated the nucleotide sequence and expression of lox2, a mutant form of the gene encoding lipoxygenase 2, an enzyme responsible for unpleasant flavors in soybean [glycine max (l) merr.] seeds. although lox2 transcripts accumulate normally, there are no detectable transcripts for lipoxygenase 1 or 3 in mutant lines that display similar phenotypes characterized by the lack of corresponding lipoxygenase activity and protein in mature seeds. the enzymatically inactive lox2 gene product ...	1994	8016074
plant delta-aminolevulinic acid dehydratase. expression in soybean root nodules and evidence for a bacterial lineage of the alad gene.	we isolated a soybean (glycine max) cdna encoding the heme and chlorophyll synthesis enzyme delta-aminolevulinic acid (ala) dehydratase by functional complementation of an escherichia coli hemb mutant, and we designated the gene alad. ala dehydratase was strongly expressed in nodules but not in uninfected roots, although alad mrna was only 2- to 3-fold greater in the symbiotic tissue. light was not essential for expression of alad in leaves of dark-grown etiolated plantlets as discerned by mrna, ...	1994	8016269
a chlorophyll a/b-binding protein gene from soybean (glycine max [l.] merr.).		1994	8016279
vitamin b12 production by citrobacter freundii or klebsiella pneumoniae during tempeh fermentation and proof of enterotoxin absence by pcr.	the influence of some fermentation parameters on vitamin b12 formation by strains of citrobacter freundii and klebsiella pneumoniae isolated from indonesian tempeh samples during tempeh fermentation was investigated. a decrease in fermentation temperature from 32 to 24 degrees c led to a decrease in vitamin b12 formation. inoculation of soybeans with different numbers of cells of c. freundii at the beginning of solid-substrate fermentation showed that only the velocity of vitamin formation and n ...	1994	8017933
characterization of glycoconjugates in the endometrium of the miniature pig during different reproductive periods.	lectin-binding glycoconjugates in the endometrium of miniature pigs were determined for sexually immature, dioestrus, oestrus and early pregnancy periods, using five horseradish peroxidase-labelled lectins. apparent changes associated with sexual maturity were increases in binding intensity of wheat germ agglutinin (wga), glycine max agglutinin (sba) and ulex europaeus agglutinin (uea) and the appearance of ricinus communis agglutinin (rca) binding. the binding intensity of wga, sba and uea furt ...	1994	8021858
sequence of a soybean (glycine max l.) phosphoenolpyruvate carboxylase cdna.		1993	8022928
experimental evidence of a plant meridian system: i. bioelectricity and acupuncture effects on electrical resistance of the soybean (glycine max).	experiments were conducted on the bioelectrical potential and resistance of soybean (glycine max) roots, stems, leaves and pods. results showed a higher potential and lower electrical resistance associated with the leaf cushion, main vein and small vein areas in comparison with other parts of the plant. when two needles were inserted into one of the low resistance points, i.e., the leaf cushion area, the electrical resistance decreased 26.0% on the main vein and 4.5% on the mesophyll of the soyb ...	1994	8030614
kinetic properties of nad-dependent glyceraldehyde-3-phosphate dehydrogenase from the host fraction of soybean root nodules.	the kinetic mechanism of nad-dependent glyceraldehyde-3-phosphate dehydrogenase [d-glyceraldehyde-3-phosphate:nad+ oxidoreductase (phosphorylating), ec 1.2.1.12] from the host cytosolic fraction of soybean (glycine max l. merr. cv. williams) root nodules has been investigated by steady-state initial velocity studies and inhibition studies with products and product analogs. the results were consistent with the enzyme having a bi-uni-uni-uni ping-pong mechanism in which nad+ and phosphate interact ...	1994	8031116
observation of unique cross-linked lattices between multiantennary carbohydrates and soybean lectin. presence of pseudo-2-fold axes of symmetry in complex type carbohydrates.	the tetrameric lectin from glycine max (soybean) (sba) has been shown to cross-link and precipitate with n-linked multiantennary complex type oligosaccharides containing nonreducing terminal gal residues (bhattacharyya, l., haraldsson, m., & brewer, c. f. (1988) biochemistry 27, 1034-1041). in the present study, negative stain electron micrographs of the precipitates of sba with a series of naturally occurring and synthetic multiantennary carbohydrates with terminal gal or galnac residues show t ...	1994	8031418
[a new russian phospholipid reagent from soybeans for the study of activated partial thromboplastin time].		2015	8032782
glycoconjugate expression during drosophila embryogenesis.	glycoproteins and other glycoconjugates present on the surface of many cell types have been identified and assigned various functions. the extent of variation possible in complex glycan structures and the heterogeneity of glycoconjugate expression between two apparently similar cells has been demonstrated previously by using plant lectins to survey developmental biological models. to examine the array and extent of glycoconjugate roles in embryonic drosophila neurogenesis, we have used plant lec ...	1994	8036876
purification and physicochemical characterization of soyatoxin, a novel toxic protein isolated from soybeans (glycine max).	physicochemical characterization and biological properties of a new toxic protein isolated from soybeans (glycine max) is reported. the purification procedure consisted basically of ammonium sulfate fractionation, ion exchange, and affinity chromatographies, the latter being used for the removal of the seed's lectin and of its trypsin inhibitor. the highly purified protein, designated soyatoxin, is a single chain acidic protein (pi 4.4-4.6) of 21 kda, dependent on reduced thiol groups to maintai ...	1994	8037448
lactational performance of dairy cows fed raw soybeans, with or without animal by-product proteins, or roasted soybeans.	twelve multiparous holstein cows averaged 10 wk postpartum and were used in a replicated 3 x 3 latin square design to compare two feeding strategies for increasing the ratio of dietary undegradable to degradable protein. treatments were raw soybeans, with or without meat and bone meal plus blood meal, and roasted soybeans as the primary protein supplements. meat and bone meal and blood meal were fed at 4.0 and .9% of dietary dm, respectively. basal diets were 30% alfalfa silage, 18% corn silage, ...	1994	8046075
in vitro determination of ruminal protein degradability using [15n]ammonia to correct for microbial nitrogen uptake.	an in vitro procedure was developed to estimate rate and extent of ruminal protein degradation using [15n]h3 to quantify uptake of protein degradation products for microbial protein synthesis. incubations were conducted for 6 h in stirrer flasks with ruminal inoculum plus buffer, reducing solution, pectin, soluble carbohydrates, and added (15nh4)2so4. seven protein concentrates were tested in the system. samples of media were analyzed for 15n enrichment of nh3, microbial n, and total solids n. d ...	1994	8056683
on a bowman-birk family proteinase inhibitor from erythrina variegata seeds.	a bowman-birk family proteinase inhibitor (ebi) was isolated from the seeds of erythrina variegata. the protein was purified by ion-exchange column chromatography on deae-cellulose followed by gel filtration on sephadex g-75. the stoichiometry with trypsin was estimated to be 1:1, while that with chymotrypsin was not obvious, as determined from the titration patterns of its inhibitory activities. the complete amino acid sequence of ebi was determined by sequencing tryptic and chymotryptic peptid ...	1994	8056744
soy intake and cancer risk: a review of the in vitro and in vivo data.	international variations in cancer rates have been attributed, at least in part, to differences in dietary intake. recently, it has been suggested that consumption of soyfoods may contribute to the relatively low rates of breast, colon, and prostate cancers in countries such as china and japan. soybeans contain a number of anticarcinogens, and a recent national cancer institute workshop recommended that the role of soyfoods in cancer prevention be investigated. in this review, the hypothesis tha ...	1994	8058523
formation of b-vitamins by bacteria during the soaking process of soybeans for tempe fermentation.	the formation of b-vitamins (nicotinic acid and nicotinamide, thiamine, vitamin b6 and vitamin b12) during the soaking of soybeans by bacteria, isolated from tempe, was investigated. among the isolates examined no vitamin b6 producer was found. after inoculation of the soaking soybeans with citrobacter freundii, klebsiella pneumoniae, pseudomonas fluorescens and streptococcus spp. the concentrations of vitamin b12 increased significantly. for the first time it was found that soakings inoculated ...	1994	8060790
cloning of a higher-plant plastid omega-6 fatty acid desaturase cdna and its expression in a cyanobacterium.	oligomers based on amino acids conserved between known plant omega-3 and cyanobacterium omega-6 fatty acid desaturases were used to screen an arabidopsis cdna library for related sequences. an identified clone encoding a novel desaturase-like polypeptide was used to isolate its homologs from glycine max and brassica napus. the plant deduced amino acid sequences showed less than 27% similarity to known plant omega-6 and omega-3 desaturases but more than 48% similarity to cyanobacterial omega-6 de ...	1994	8066133
chalcone synthase mrna and activity are reduced in yellow soybean seed coats with dominant i alleles.	the seed of all wild glycine accessions have black or brown pigments because of the homozygous recessive i allele in combination with alleles at the r and t loci. in contrast, nearly all commercial soybean (glycine max) varieties are yellow due to the presence of a dominant allele of the i locus (either i or i) that inhibits pigmentation in the seed coats. spontaneous mutations to the recessive i allele occur in these varieties and result in pigmented seed coats. we have isolated a clone for a s ...	1994	8066134
effects of extruded soybeans and forage source on fermentation by rumen microorganisms in continuous culture.	continuous culture fermenters were used to evaluate effects of extrusion of whole soybeans and changes in forage composition of diets on microbial fermentation. treatments were arranged in a 2 x 4 factorial design with soybeans (raw or extruded) and dietary treatment (ratio of alfalfa hay to corn silage; 82:18, 61:39, 43:57, and 27:73 of dietary forage) as main effects. soybeans constituted 9.6, 14.4, 19.2, and 23.9% of dm for each of the respective dietary treatments. true digestion of dm, adf, ...	1994	8083419
nucleotide sequence and putative regulatory elements of gene 2 of the soybean (glycine max) chalcone synthase multigene family.		1993	8108500
nucleotide sequence and putative regulatory elements of a nodule-development-specific member of the soybean (glycine max) chalcone synthase multigene family, gmchs 7.		1993	8108501
identification, characterization, and analysis of cdna and genomic sequences encoding two different small heat shock proteins in hordeum vulgare.	in vitro translation of mrnas prepared from barley (hordeum vulgare) seedlings (cv. onice) exposed at 40 degrees c directed the synthesis of major heat shock proteins (hsps) with molecular masses of 80-90, 70, 42 and 16-22 kda. a cdna library prepared from the 40 degrees c mrnas and screened by differential hybridization led to the isolation of heat shock specific sequences. one of these (hv hsp18) was confirmed by hybrid-arrested and hybrid-released translation as encoding for an 18-kda hsp. th ...	1993	8112573
characterization of a soybean beta-conglycinin-degrading protease cleavage site.	protease c1, an enzyme from soybean (glycine max [l.] merrill cv amsoy 71) seedling cotyledons, was previously determined to be the enzyme responsible for the initial degradation of the alpha' and alpha subunits, but not the beta subunit, of beta-conglycinin storage protein. the sizes of the proteolytic products generated by the action of protease c1 suggest that the cleavage sites on the alpha' and alpha subunits of beta-conglycinin may be located in their n-terminal domain, which is not found ...	1994	8115542
molecular cloning and characterization of a brassinosteroid-regulated gene from elongating soybean (glycine max l.) epicotyls.	brassinosteroids promote elongation and regulate gene expression in soybean (glycine max l.) stems. we constructed a cdna library from brassinosteroid-treated soybean epicotyls and used differential hybridization to isolate a cdna (pbru1) corresponding to a transcript whose abundance is increased by brassinosteroid treatment. sequence analysis of pbru1 revealed an open reading frame of 283 amino acids with a putative signal peptide of 29 amino acids. the sequence had extensive homology (77% iden ...	1994	8115544
posttranscriptional regulation of ferritin during nodule development in soybean.	during soybean (glycine max) nodule development, induced ferritin mrna concentration remains elevated while the protein concentration decreases 4- to 5-fold (m. ragland and e.c. theil [1993] plant mol biol 21: 555-560). investigation of posttranscriptional regulation of nodule ferritin during development showed that ferritin mrna was efficiently translated based on polyribosome size in vivo, protein synthesis (0.8% of total protein) in vitro, and protein synthesis in intact nodules. ferritin, a ...	1994	8115547
raw soybeans and roasted soybeans of different particle sizes. 2. fatty acid utilization by lactating cows.	this study was conducted to examine the effects of feeding whole raw soybeans and roasted soybeans of different particle sizes on utilization by ruminal bacteria and lactating cows. five cows with ruminal and duodenal cannulas were used in a 5 x 5 latin square design. diets contained 55% corn silage and 45% concentrate. treatments were control (ca soaps at 4.24% of dietary dm), whole raw soybeans, whole roasted soybeans, cracked roasted soybeans, or ground roasted soybeans. all soybean diets con ...	1994	8120185
role of intramolecular high-mannose chains in the folding and assembly of soybean (glycine max) lectin polypeptides: studies by the combined use of spectroscopy and gel-filtration size analysis.	it was previously reported [nagai, k. & yamaguchi, h. (1993) j. biochem. 113, 123-125] that intramolecular high-mannose chains are essential for reconstitution of soybean lectin from denatured subunits. to obtain more detailed information on the role of the intramolecular high-mannose chains in the folding and assembly of soybean lectin polypeptides, the effects of asparagine-linked oligosaccharides, man9glcnac2asn (m9-asn) and glc1-3man9glcnac2asn (gm9-asn), on the reconstitution of soybean lec ...	1993	8138539
implications of antinutritional components in soybean foods.	there are a number of components present in soybeans that exert a negative impact on the nutritional quality of the protein. among those factors that are destroyed by heat treatment are the protease inhibitors and lectins. protease inhibitors exert their antinutritional effect by causing pancreatic hypertrophy/hyperplasia, which ultimately results in an inhibition of growth. the lectin, by virtue of its ability to bind to glycoprotein receptors on the epithelial cells lining the intestinal mucos ...	1994	8142044
the dioxygenation rate in lipoxygenase catalysis is determined by the amount of iron (iii) lipoxygenase in solution.	the dioxygenation rate in reactions catalyzed by lipoxygenase-1 from soybeans has been measured as a function of the enzyme present in the fe(iii) form with rapid kinetic techniques. the experiments were carried out at ph 10, 25 degree c. the product concentration and the fraction of iron (iii) lipoxygenase were monitored by measuring the absorbance at 243 nm and the tryptophan fluorescence at 330 nm (excitation at 287 nm), respectively. in reactions started with 1.3 microm iron (ii) lipoxygenas ...	1994	8142401
ulex europaeus i and glycine max bind to the human olfactory bulb.	the distribution of binding sites for the fucose-selective lectin ulex europaeus i and the terminal n-acetylgalactosamine-selective lectin glycine max in the human olfactory bulb were studied. these lectins bound to primary olfactory axons in the olfactory nerve layer and the glomerular layer. they also bound to fibers located in the deeper layers such as the external plexiform layer and the granular layer. furthermore they projected to the olfactory stalk but not in the cerebrum. the deeper pro ...	1993	8152605
cloning and sequence analysis of a cdna encoding ferric leghemoglobin reductase from soybean nodules.	a cdna encoding soybean (glycine max [l.] merr) ferric leghemoglobin reductase (flbr), an enzyme that is postulated to play an important role in maintaining leghemoglobin in its functional ferrous state, has been cloned and characterized. a group of highly degenerate oligonucleotides deduced from the n-terminal amino acid sequence of flbr was used to prime the polymerase chain reaction (pcr) on soybean nodule mrna and cdna. a full-length clone of flbr cdna was isolated by screening a lambda gt11 ...	1994	8159785
comparative study of cellulases associated with adventitious root initiation, apical buds, and leaf, flower, and pod abscission zones in soybean.	cellulase activity was measured in soybean (glycine max) leaf abscission zones, flower abscission zones, pod abscission zones, apical buds, and adventitious rooting hypocotyls. immunoprecipitation data showed that a cellulase immunologically similar to the bean abscission cellulase (isoelectric point 9.5) is present in soybean leaf, flower, and pod abscission zones, but is not present in soybean apical buds or rooting hypocotyls. cdna and genomic clones for two different soybean genes were ident ...	1994	8159787
isolation and characterization of three soybean extensin cdnas.	we have characterized three different soybean (glycine max) mrnas that encode apoproteins of extensins, a family of cell wall hydroxyproline-rich glycoproteins (hrgps). these transcripts encoded distinctive tyr-rich proteins containing characteristic ser-pro4 sequences organized in higher-order repetitive units. the first transcript encoded an extensin sbhrgp-1 containing the 16-amino acid repeat ser-pro4-ser-pro-ser-pro4-tyr-val-tyr-lys, with val occasionally replaced by ile or tyr. the second ...	1994	8159793
nucleotide sequence of a soybean (glycine max l. merr.) ubiquitin gene.		1994	8159798
observation of unique cross-linked lattices between multiantennary carbohydrates and soybean lectin. presence of pseudo-2-fold axes of symmetry in complex type carbohydrates.	the tetrameric lectin from glycine max (soybean) (sba) has been shown to cross-link and precipitate with n-linked multiantennary complex type oligosaccharides containing nonreducing terminal gal residues (bhattacharyya, l., haraldsson, m., & brewer, c. f. (1988) biochemistry 27, 1034-1041). in the present study, negative stain electron micrographs of the precipitates of sba with a series of naturally occurring and synthetic multiantennary carbohydrates with terminal gal or galnac residues show t ...	1994	8180186
hypocotyl expression and light downregulation of the soybean tubulin gene, tubb1.	the tubb1 beta-tubulin gene of glycine max (previously named s beta 1) is highly expressed only in rapidly elongating regions of etiolated seedling hypocotyls and this expression is strongly downregulated when the seedlings are exposed to light. primer extension demonstrated that the gene was transcribed in these tissues and contained two sites of transcriptional initiation. to determine the mechanism regulating tubb1 expression, a chimeric reporter gene was constructed by fusing 5' upstream reg ...	1994	8180620
variable stability of antibiotic-resistance markers in bacillus cereus uw85 in the soybean rhizosphere in the field.	we compared the stability of antibiotic-resistance markers in strains derived from bacillus cereus uw85 in culture media and in the soybean rhizosphere in a growth chamber and in the field. we studied two independent, spontaneous mutants resistant to neomycin, three independent, spontaneous mutants resistant to streptomycin, and strains carrying plasmid pbc16, which encodes tetracycline resistance. antibiotic-resistance markers were maintained in populations of all uw85 derivatives in culture an ...	1993	8180735
infection of soybean and pea nodules by rhizobium spp. purine auxotrophs in the presence of 5-aminoimidazole-4-carboxamide riboside.	purine auxotrophs of various rhizobium species are symbiotically defective, usually unable to initiate or complete the infection process. earlier studies demonstrated that, in the rhizobium etli-bean symbiosis, infection by purine auxotrophs is partially restored by supplementation of the plant medium with 5-amino-imidazole-4-carboxamide (aica) riboside, the unphosphorylated form of the purine biosynthetic intermediate aicar. the addition of purine to the root environment does not have this effe ...	1994	8195084
lectins as markers of rumen epithelial cell differentiation.	lectins of different carbohydrate specificities (gna (galanthus nivalis), con a (canavalia ensiformis), vfl (vicia faba), psl (pisum sativum), lca (lens culinaris), pna (arachis hypogaea; with or without prior neuraminidase treatment), wga (triticum vulgare), sba (glycine max), uea-i (ulex europaeus), lpa (limulus polyphemus), bs-i b4 (bandeiraea simplicifolia, isolectin b4)) were explored for use as differentiation markers of rumen epithelial cells in vivo and in vitro. lectins specific for man ...	1994	8206789
phytogrowth-inhibitory activities of sulfur-containing compounds. ii. the inhibitory activities of thiosalicylic acid and dihydro-2(3h)-thiophenone-related compounds on plant growth.	thiosalicylic acid (i) showed rather strong inhibitory activity on the growth of roots of all plants treated except abelmoschus esculentus moench at the concentration of 5.0 x 10(-4) m. this compound strongly inhibited the growth of the root of echinochloa utilis ohwi et yabuno even at the low concentration of 5.0 x 10(-5) m. dihydro-2(3h)-thiophenone (vii) also exhibited inhibitory activity on the growth of roots of all plants treated except glycine max merrill. both compounds inhibited the ger ...	1993	8220332
[reconsideration of the role of aflatoxins in public health].	the pathogenicity of aflatoxins for human has been largely demonstrated. although aflatoxin contamination of some foodstuffs has been unquestionably established, stress should be laid upon a certain lack of information: some of the affected foodstuffs have been "forgotten" in the enumeration of contaminated products (especially soybeans); relations between mycotoxins and food balance have not yet been taken into account: for instance, the influence of some vitamins, of various compounds (caroten ...	1993	8221179
rumen fermentation and lactation performance of cows fed roasted soybeans and tallow.	sixteen multiparous holstein cows were in a replicated 4 x 4 latin square design with 21-d periods to examine the effects of incremental tallow addition to diets containing whole roasted soybeans on rumen fermentation and lactational performance. total mixed rations were fed for ad libitum intake and contained, on a dm basis, 33% alfalfa silage, 12% corn silage, 14% roasted soybeans, and 41% concentrate based on ground corn and soybean meal. treatments were 0, 1, 2, or 3% supplemental tallow. di ...	1993	8227669
lupins (lupinus albus) as a protein supplement for lactating holstein dairy cows.	three experiments were conducted to determine optimal substitution of lupin for soybean meal in dairy cattle diets; the effects of whole versus ground lupins and the effects of whole lupins versus whole soybeans on milk production were examined. in the first experiment, 57 cows were assigned randomly to treatments in blocks of five by calving order within parity. treatments began 22 d postpartum and continued through d 140. diets were isonitrogenously balanced using soybean meal as control: lupi ...	1993	8227670
occurrence of antimicrobial activities of bacteria from soybean leaf spots.	bacteria were isolated from leaf spots of field grown soybeans during two growing seasons. the leaf spots yielded up to 4 different species and a total population size of about 10(7)-10(8) bacteria/cm2. the majority of the 192 isolates belonged to the species pseudomonas syringae pv. glycinea (55%), causing leaf spots of bacterial blight on soybeans, and erwinia herbicola (22%). the remaining isolates included bacteria from other genera, but occurred occasionally. the determination of biological ...	1993	8229675
enhanced detection of polymorphic dna by multiple arbitrary amplicon profiling of endonuclease-digested dna: identification of markers tightly linked to the supernodulation locus in soybean.	multiple endonuclease digestion of template dna or amplification products can increase significantly the detection of polymorphic dna in fingerprints generated by multiple arbitrary amplicon profiling (maap). this coupling of endonuclease cleavage and amplification of arbitrary stretches of dna, directed by short oligonucleotide primers, readily allowed distinction of closely related fungal and bacterial isolates and plant cultivars. maap analysis of cleaved template dna enabled the identificati ...	1993	8232212
preventing asthma epidemics due to soybeans by dust-control measures.	the inhalation of soybean dust released during the unloading of soybeans into a silo caused outbreaks of asthma in barcelona, spain. this study was designed to determine morbidity due to asthma and serum ige reactivity before and after the installation of filters in the silo.	1993	8232484
cancer prevention by protease inhibitors.	there is now compelling evidence that certain protease inhibitors have strong anticarcinogenic activity. a particular protease inhibitor derived from soybeans, the bowman-birk inhibitor, appears to be highly promising as a cancer chemopreventive agent. in this article, studies performed to characterize an extract of soybeans containing the bowman-birk inhibitor, termed bowman-birk inhibitor concentrate, are described.	1993	8234219
miso from peas (pisum sativum) and beans (phaseolus vulgaris) of domestic origin. fermented foods from agricultural products in europe. ii.	miso is a fermented soybean paste widely used in japan as a soup base or as a seasoning agent. koji (cereal grains with the mold aspergillus oryzae) serves as enzyme source. peas (pisum sativum) and beans (phaseolus vulgaris) of german origin can be used as substitutes for soybeans in the preparation of miso-like products. the legumes (peas, beans and soybeans for comparison) are washed, soaked in boiled water, dehulled and cooked for 35 min at reduced pressure. after grounding the seeds are mix ...	1993	8237083
molecular cloning and cdna sequencing of endoxyloglucan transferase, a novel class of glycosyltransferase that mediates molecular grafting between matrix polysaccharides in plant cell walls.	endoxyloglucan transferase is a novel class of glycosyltransferase recently purified from vigna angularis (nishitani, k,, and tominaga, r. (1992) j. biol. chem. 267, 21058-21064). this enzyme is the first transferase identified that catalyzes molecular grafting between polysaccharide cross-links in the cell wall matrix and participates in reconstruction of the network structure in the cell wall. based on the nh2-terminal amino acid sequence information of the purified transferase, we have here c ...	1993	8244968
analysis of soybean chloroplast dna replication by two-dimensional gel electrophoresis.	chloroplast dna replication was studied in the green, autotrophic suspension culture line sb-1 of glycine max. three regions (restriction fragments sac i 14.5, pvu ii 4.1 and pvu ii 14.8) on the plastome were identified that displayed significantly higher template activity in in vitro dna replication assays than all other cloned restriction fragments of the organelle genome, suggesting that these clones contain sequences that are able to direct initiation of dna replication in vitro. in order to ...	1993	8251631
sire-1, a long interspersed repetitive dna element from soybean with weak sequence similarity to retrotransposons: initial characterization and partial sequence.	gm776 is a 776-bp subregion of a member of an interspersed family of relatively homogeneous repetitive dna elements from soybean (glycine max). the fragment was originally amplified from soybean dna by the polymerase chain reaction using a single 22-nucleotide primer, and consequently terminates in an inverted repeat. the elements defined by gm776 are at least 10.6 kb in length and constitute a family of 500-800 members per haploid genome. the family has been designated sire-1 (soybean intersper ...	1993	8262373
tempeh: a mold-modified indigenous fermented food made from soybeans and/or cereal grains.	a variety of indigenous fermented foods exist today; however, tempeh has been one of the most widely accepted and researched mold-modified fermented products. tempeh is a traditional fermented food made from soaked and cooked soybeans inoculated with a mold, usually of the genus rhizopus. after fermentation has occurred, the soybeans are bound together into a compact cake by dense cottony mycelium. an important function of the mold in the fermentation process is the synthesis of enzymes, which h ...	1993	8267862
expression of the soybean (glycine max) glutamate 1-semialdehyde aminotransferase gene in symbiotic root nodules.	extracts of soybean (glycine max) root nodules and greening etiolated leaves catalyzed radiolabeled delta-aminolevulinic acid (ala) formation from 3,4-[3h]glutamate but not from 1-[14c]glutamate. nevertheless, those tissue extracts expressed the activity of glutamate 1-semialdehyde (gsa) aminotransferase, the c5 pathway enzyme that catalyzes ala synthesis from gsa for tetrapyrrole formation. a soybean nodule cdna clone that conferred ala prototrophy, gsa aminotransferase activity, and glutamate- ...	1993	8278535
molecular cloning and expression of 4-coumarate:coenzyme a ligase, an enzyme involved in the resistance response of soybean (glycine max l.) against pathogen attack.	we have isolated three classes of cdnas that probably encode three 4-coumarate:coenzyme a ligase (4cl) isoenzymes in soybean (glycine max l.). the deduced amino acid sequences reveal several regions of extended sequence identity among 4cls of all plants analyzed to date. the sequences of two of these regions are consistent with a domain structure proposed for a group of enzymes catalyzing the atp-dependent covalent binding of amp to their substrates during the reaction sequence. by using two cdn ...	1993	8278545
histochemical analysis of rat testicular glycoconjugates. 3. non-reducing terminal residues in seminiferous tubules.	lectins of helix pomatia (hpa), glycine max (sba), vicia villosa (vva), dolichos biflorus (dba), ulex europaeus (uea-1), tetragonolobus purpureus (lta), griffonia simplicifolia (bsa-1b4), maclura pomifera (mpa), sambucus nigra (sna) and maackia amurensis (maa) were used to explore the distribution of saccharides characteristic of non-reducing termini of o- and n-linked glycoprotein glycans in the seminiferous tubules of rat testis. sialyl residues (both alpha 2,3- and alpha 2,6-linked, as shown ...	1993	8282567
solubilization of functional plasma membrane-localized hepta-beta-glucoside elicitor-binding proteins from soybean.	total membranes prepared from roots of soybean (glycine max l.) seedlings have previously been shown to contain proteinaceous binding site(s) for a hepta-beta-glucoside elicitor of phytoalexin accumulation. the hepta-beta-glucoside elicitor-binding proteins have now been shown to co-migrate with a plasma membrane marker enzyme (vanadate-sensitive h(+)-atpase) on linear sucrose density gradients. with the use of detergents, the elicitor-binding proteins have been solubilized in functional form fr ...	1993	8290628
purification of allantoinase from soybean seeds and production and characterization of anti-allantoinase antibodies.	allantoinase catalyzes the hydrolysis of allantoin to allantoic acid, a reaction important in both biogenesis and degradation of ureides. ureide production in cotyledons of germinating soybean (glycine max l.) seeds has not been studied extensively but may be important in mobilizing nitrogen reserves. allantoinase was purified approximately 2500-fold from a crude extract of soybean seeds by differential centrifugation, heat treatment, ammonium sulfate fractionation, ethanol fractionation, and fa ...	1993	8290630
effects of extrusion and expelling on the nutritional quality of conventional and kunitz trypsin inhibitor-free soybeans.	two chick growth experiments and a precision-fed cockerel digestibility assay were conducted to evaluate the effect of extrusion and expelling on the nutritive value of conventional (csb) and kunitz trypsin inhibitor-free (kfsb) soybeans. in the first experiment, performance of chicks fed csb or kfsb autoclaved at 121 c was similar to that of chicks fed csb or kfsb extruded at 138 c. the effect of extrusion temperature on protein quality of the soybeans was evaluated in the second experiment. el ...	1993	8309879
nucleotide sequences of cdnas encoding the precursors for soybean (glycine max) trypsin inhibitors (kunitz type).		1993	8310071
studies on the constancy of the blue and green fluorescence yield during the chlorophyll fluorescence induction kinetics (kautsky effect).	blue (f450) and green (f530) leaf fluorescence were studied together with the red chlorophyll fluorescence (emission maxima f690 and f735) during light-induced chlorophyll fluorescence induction kinetics (kautsky effect) in predarkened leaves of wheat (triticum aestivum l.) and soybean (glycine max l.). the intensity of the red chlorophyll fluorescence decreased from maximum fluorescence fm to steady-state fluorescence fs, and the fluorescence ratio f690/f735 decreased by about 10% from fm to fs ...	1993	8310130
lectin histochemistry of normal and neoplastic peripheral nerve sheath. 1. lectin binding pattern of normal peripheral nerve in man.	the binding patterns of lectins to normal peripheral nerves were examined. twelve biotinylated lectins were used in this study; canavalia ensiformis (con a), pisum sativum (psa), lens culinaris (lca), ricinus communis 1 (rca-1), arachis hypogaea (pna), glycine max (sba), sophora japonica (sja), bandeiraea simplicifolia 1 (bsl-1), triticum vulgaris (wga), succinylated wga (s-wga), ulex europaeus 1 (uea-1) and helix pomatia (hpa). cytoplasm of schwann cells and perineurial cells was stained by con ...	1993	8310810
lectin histochemistry of normal and neoplastic peripheral nerve sheath. 2. lectin binding patterns of schwannoma and neurofibroma.	lectin binding patterns of 31 schwannomas and 6 neurofibromas were examined using 12 lectins, and the results were compared with those of normal peripheral nerves. tumors obtained from 10 cases of neurofibromatosis and 4 recurrent schwannomas were included. changes of glycoconjugates were observed in association with a neoplastic transformation of schwann cells; arachis hypogaea (pna) staining after neuraminidase treatment seen in normal schwann cells was reduced in schwannoma of antoni type a, ...	1993	8310811
physical mapping of a region in the soybean (glycine max) genome containing duplicated sequences.	pulsed-field gel electrophoresis (pfge) was used to study a cluster of molecular markers in the soybean genome. there were 550 kb per centimorgan (cm) in the cluster, which is close to the calculated average for the whole genome. the analysis was complicated by the presence of duplicated sequences, and some ambiguities arising from this were resolved by using second-dimension conventional electrophoresis to relate physical maps to the rflp map of soybean. the results show that there is a high de ...	1993	8329683