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effect of top soil wettability on water evaporation and plant growth.in general, agricultural soil surfaces being hydrophilic in nature get easily wetted by water. the water beneath the soil moves through capillary effect and comes to the surface of the soil and thereafter evaporates into the surrounding air due to atmospheric conditions such as sunlight, wind current, temperature and relative humidity. to lower the water loss from soil, an experiment was designed in which a layer of hydrophobic soil was laid on the surface of ordinary hydrophilic soil. this tech ...201525801650
bioinformatics approaches for viral metagenomics in plants using short rnas: model case of study and application to a cicer arietinum population.over the past years deep sequencing experiments have opened novel doors to reconstruct viral populations in a high-throughput and cost-effective manner. currently a substantial number of studies have been performed which employ next generation sequencing techniques to either analyze known viruses by means of a reference-guided approach or to discover novel viruses using a de novo-based strategy. taking advantage of the well-known cymbidium ringspot virus we have carried out a comparison of diffe ...201425674078
zinc-induced modulation of some biochemical parameters in a high- and a low-zinc-accumulating genotype of cicer arietinum l. grown under zn-deficient condition.this study presents a comparative account of biochemical responses evoked by a high-zinc-accumulating (hzng) and a low-zinc-accumulating (lzng) genotype of chickpea (cicer arietinum l.) under conditions of zinc deficiency (0 mg znso4 kg(-1) soil) and on exogenous zinc supply (2.5, 5, and 10 mg znso4 kg(-1) soil), at different growth stages (pre-flowering, flowering, and post-flowering) of the crop. both the genotypes exhibited significant variation in contents of chlorophyll, protein, nitrate, a ...201525673554
isoflavone content and composition in chickpea (cicer arietinum l.) sprouts germinated under different conditions.the influence of different germination conditions on isoflavone contents in chickpea sprouts was investigated in this study. chickpea sprouts were germinated under different experimental conditions, including germination in the dark (gd), in the light (gl), under ethanol stress (ge), or under salt stress (gs) in the dark. the results demonstrated that the isoflavone contents in chickpea sprouts germinated with these various conditions significantly increased (p<0.05) compared to those in untreat ...201525630489
dpph radical scavenging activity and contents of h2o2, malondialdehyde and proline in determining salinity tolerance in chickpea seedlings.the involvement of 1,1-diphenyl-2-picrylhydrazyl (dpph) radical scavenging activity and contents of h2o2, malondialdehyde (mda) and proline was investigated in determining salinity tolerance among seedlings of thirty chickpea (cicer arietinum l.) genotypes having different pedigrees. chickpea genotypes, including cultivars and advanced lines were grown for 7 days under control and salt stress (50 mm nacl) conditions. the genotypes showed differential response to salt stress in terms of growth, d ...201425630111
salt sensitivity in chickpea (cicer arietinum l.): ions in reproductive tissues and yield components in contrasting genotypes.the reproductive phase in chickpea (cicer arietinum l.) is affected by salinity, but little is known about the underlying cause. we investigated whether high concentrations of na(+) and cl(-) in the reproductive structures influence reproductive processes. chickpea genotypes contrasting in tolerance were subjected to 0, 35 or 50 mm nacl applied to soil in pots. flower production and abortion, pod number, percentage of empty pods, seed number and size were evaluated. the concentrations of na(+) , ...201525615287
anticonvulsant effect of cicer arietinum seed in animal models of epilepsy: introduction of an active molecule with novel chemical structure.cicer arietinum (chickpea) is one of the most important harvests in the world with high nutritional value. lack of essential oils in the seeds of chickpea is an advantage in search for drug-like molecules with less toxicity. we evaluated anticonvulsant effect of c. arietinum in common animal models of epilepsy.201525605489
global transcriptome analysis of developing chickpea (cicer arietinum l.) seeds.understanding developmental processes, especially in non-model crop plants, is extremely important in order to unravel unique mechanisms regulating development. chickpea (c. arietinum l.) seeds are especially valued for their high carbohydrate and protein content. therefore, in order to elucidate the mechanisms underlying seed development in chickpea, deep sequencing of transcriptomes from four developmental stages was undertaken. in this study, next generation sequencing platform was utilized t ...201425566273
crystal structure of a plant albumin from cicer arietinum (chickpea) possessing hemopexin fold and hemagglutination activity.crystal structure of a reported pa2 albumin from cicer arietinum shows that it belongs to hemopexin fold family, has four beta-propeller motifs and possesses hemagglutination activity, making it different from known legume lectins. a plant albumin (pa2) from cicer arietinum, presumably a lectin (cal) owing to its hemagglutination activity which is inhibited by complex sugars as well as glycoproteins such as fetuin, desialylated fetuin and fibrinogen. the three-dimensional structure of this homod ...201525559942
effect of plant growth-promoting streptomyces sp. on growth promotion and grain yield in chickpea (cicer arietinum l).the physiological and molecular responses of six strains of streptomyces sp. (cai-13, cai-85, cai-93, cai-140, cai-155 and kai-180), with their proven potential for plant growth-promotion (pgp) in rice were studied to understand the mechanisms causing the beneficial effects. in this investigation, those six strains were evaluated for their pgp capabilities in chickpea in the 2012-13 and 2013-14 post-rainy seasons. all of the streptomyces sp. strains exhibited enhanced nodule number, nodule weigh ...201528324533
genetic mapping of legume orthologs reveals high conservation of synteny between lentil species and the sequenced genomes of medicago and chickpea.lentil (lens culinaris medik.) is a global food crop with increasing importance for food security in south asia and other regions. lens ervoides, a wild relative of cultivated lentil, is an important source of agronomic trait variation. lens is a member of the galegoid clade of the papilionoideae family, which includes other important dietary legumes such as chickpea (cicer arietinum) and pea (pisum sativum), and the sequenced model legume medicago truncatula. understanding the genetic structure ...201425538716
the chickpea genomic web resource: visualization and analysis of the desi-type cicer arietinum nuclear genome for comparative exploration of legumes.availability of the draft nuclear genome sequences of small-seeded desi-type legume crop cicer arietinum has provided an opportunity for investigating unique chickpea genomic features and evaluation of their biological significance. the increasing number of legume genome sequences also presents a challenge for developing reliable and information-driven bioinformatics applications suitable for comparative exploration of this important class of crop plants.201425518738
physicochemical characterization of sewage sludge and green waste for agricultural utilization.in order to valorize the organic wastes, a mixture composed of 60 kg of thick sewage sludge from a wastewater treatment plant, 30 kg of green wastes (made of 10 kg straw of wheat, 10 kg manure farm wastes, and 10 kg of dead leaves), and 10 kg of wood chips was prepared. the organic wastes were mixed and put into a wooden cubic composter having a volume of 1.5 m3. physicochemical analyses were made every 30 days for five months. the results of the analyses showed that the obtained compost had goo ...201525517858
genome-wide conserved non-coding microsatellite (cnms) marker-based integrative genetical genomics for quantitative dissection of seed weight in chickpea.phylogenetic footprinting identified 666 genome-wide paralogous and orthologous cnms (conserved non-coding microsatellite) markers from 5'-untranslated and regulatory regions (urrs) of 603 protein-coding chickpea genes. the (ct)n and (ga)n cnms carrying ctrmcamv35s and gaga8bkn3 regulatory elements, respectively, are abundant in the chickpea genome. the mapped genic cnms markers with robust amplification efficiencies (94.7%) detected higher intraspecific polymorphic potential (37.6%) among genot ...201525504138
genome-wide identification and expression analysis of the canac family members in chickpea during development, dehydration and aba treatments.the plant-specific nac transcription factors (tfs) play important roles in regulation of diverse biological processes, including development, growth, cell division and responses to environmental stimuli. in this study, we identified the members of the nac tf family of chickpea (cicer arietinum) and assess their expression profiles during plant development and under dehydration and abscisic acid (aba) treatments in a systematic manner. seventy-one canac genes were detected from the chickpea genom ...201425479253
effect of dehusking and cooking on protein and dietary fibre of different genotypes of desi, kabuli and green type chickpeas (cicer arietinum).dehusking and cooking are essential and important component of processing of chickpea to enhance the digestibility of essential nutrients of grains. protein and dietary fibres are important due to their role in lowering plasma cholesterol and other health advantages. changes during dehusking and dehusking followed by cooking on soluble protein, cellulose, hemicellulose, lignin and pectin contents of four genotypes of desi type (bg 256, jg 74, kwr 108 and dcp 92-3), four genotypes of kabuli type ...201425477686
genetic diversity and association mapping of iron and zinc concentrations in chickpea (cicer arietinum l.).chickpea (cicer arietinum l.) is the world's second most important pulse crop after common bean. chickpea has historically been an important daily staple in the diet of millions of people, especially in the developing countries. current chickpea breeding programs have mainly been directed toward high yield, biotic and abiotic stress resilience that has increased global production, but less attention has been directed toward improving micronutrient concentrations in seeds. in an effort to develop ...201425434748
aluminum stress inhibits root growth and alters physiological and metabolic responses in chickpea (cicer arietinum l.).chickpea (cicer arietinum l.) roots were treated with aluminum (al3+) in calcium chloride (cacl2) solution (ph 4.7) and growth responses along with physiological and metabolic changes were investigated. al3+ treatment for 7d resulted in a dose dependent decline of seed germination and inhibition of root growth. a significant (p ≤ 0.05) decline in fresh and dry biomass were observed after 7d of al3+ stress.the root growth (length) was inhibited after 24 and 48 h of stress imposition. the hydrogen ...201425394801
bioaccessibility of polyphenols from wheat (triticum aestivum), sorghum (sorghum bicolor), green gram (vigna radiata), and chickpea (cicer arietinum) as influenced by domestic food processing.cereals (wheat and sorghum) and legumes (green gram and chickpea) commonly consumed in asia and africa were evaluated for polyphenolic content. bioaccessibility of polyphenols from these grains as influenced by domestic processing was also estimated. total polyphenol content of wheat and sorghum was 1.20 and 1.12 mg/g respectively, which was increased by 49% and 20% respectively, on roasting. in contrast, a significant reduction of the same was observed in both the cereals after pressure-cooking ...201425340251
an integrated genomic approach for rapid delineation of candidate genes regulating agro-morphological traits in chickpea.the identification and fine mapping of robust quantitative trait loci (qtls)/genes governing important agro-morphological traits in chickpea still lacks systematic efforts at a genome-wide scale involving wild cicer accessions. in this context, an 834 simple sequence repeat and single-nucleotide polymorphism marker-based high-density genetic linkage map between cultivated and wild parental accessions (cicer arietinum desi cv. icc 4958 and cicer reticulatum wild cv. icc 17160) was constructed. th ...201425335477
utilization of extrusion technology for the development of millet based complementary foods.millet based complementary foods were developed using sorghum (sorghum vulgare), rice (oryza sativa), besan (cicer arietinum; bengal gram dhal flour), legume mix (green gram and roasted bengal gram; phaseolus aureus roxb and cicer arietinum) and soybean (glycine max merr) with a lab scale twin screw extruder. the extrudates were subjected to acceptability studies initially and at the end of the storage period i.e. 3 months at laboratory level by panel of judges using a 5-point hedonic scale. phy ...201425328236
dreb1a overexpression in transgenic chickpea alters key traits influencing plant water budget across water regimes.we demonstrate the role of dreb1a transcription factor in better root and shoot partitioning and higher transpiration efficiency in transgenic chickpea under drought stress chickpea (cicer arietinum l.) is mostly exposed to terminal drought stress which adversely influences its yield. development of cultivars for suitable drought environments can offer sustainable solutions. we genetically engineered a desi-type chickpea variety to ectopically overexpress atdreb1a, a transcription factor known t ...201525326370
cold stress alters transcription in meiotic anthers of cold tolerant chickpea (cicer arietinum l.).cold stress at reproductive phase in susceptible chickpea (cicer arietinum l.) leads to pollen sterility induced flower abortion. the tolerant genotypes, on the other hand, produce viable pollen and set seed under cold stress. genomic information on pollen development in cold-tolerant chickpea under cold stress is currently unavailable.201425306382
genome-wide identification and tissue-specific expression analysis of udp-glycosyltransferases genes confirm their abundance in cicer arietinum (chickpea) genome.udp-glycosyltransferases (ec 2.4.1.x; ugts) are enzymes coded by an important gene family of higher plants. they are involved in the modification of secondary metabolites, phytohormones, and xenobiotics by transfer of sugar moieties from an activated nucleotide molecule to a wide range of acceptors. this modification regulates various functions like detoxification of xenobiotics, hormone homeostasis, and biosynthesis of secondary metabolites. here, we describe the identification of 96 ugt genes ...201425290312
isoflavones extracted from chickpea cicer arietinum l. sprouts induce mitochondria-dependent apoptosis in human breast cancer cells.isoflavones are important chemical components of the seeds and sprouts of chickpeas. we systematically investigated the effects of isoflavones extracted from chickpea sprouts (ics) on the human breast cancer cell lines skbr3 and michigan cancer foundation-7 (mcf-7). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays showed that ics (10-60 µg/ml) significantly inhibited the proliferation of both cell lines in a time-dependent and dose-dependent fashion. wright-giemsa staining as ...201525287332
the carerf genes in chickpea (cicer arietinum l.) and the identification of carerf116 as abiotic stress responsive transcription factor.the ap2/erf family is one of the largest transcription factor gene families that are involved in various plant processes, especially in response to biotic and abiotic stresses. complete genome sequences of one of the world's most important pulse crops chickpea (cicer arietinum l.), has provided an important opportunity to identify and characterize genome-wide erf genes. in this study, we identified 120 putative erf genes from chickpea. the genomic organization of the chickpea erf genes suggested ...201525274312
diverse functional evolution of serine decarboxylases: identification of two novel acetaldehyde synthases that uses hydrophobic amino acids as substrates.type ii pyridoxal 5'-phosphate decarboxylases are an important group of phylogenetically diverse enzymes involved in amino acid metabolism. within plants, this group of enzymes is represented by aromatic amino acid decarboxylases, glutamate decarboxylases and serine decarboxylases. additional evolutionary divergence of plant aromatic amino acid decarboxylases has resulted in further subcategories with distinct substrate specificities and enzymatic activities. despite shared homology, no such evo ...201425230835
the role of ferredoxin:thioredoxin reductase/thioredoxin m in seed germination and the connection between this system and copper ion toxicity.seed germination is highly sensitive to changes in the surrounding environment. this work examined the impact of imbibition with copper solution on the germination rate and behavior of some enzyme capacities involved in stress response. chickpea (cicer arietinum l.) seeds were germinated at 25°c in the dark for 7 days of imbibition with distilled water or an aqueous solution of chloride salt of 100 or 500μm cucl2. the exposure of seeds to copper (cu(2+)) induced changes in the antioxidant status ...201425173453
genome-wide discovery and differential regulation of conserved and novel micrornas in chickpea via deep sequencing.micrornas (mirnas) are essential components of complex gene regulatory networks that orchestrate plant development. although several genomic resources have been developed for the legume crop chickpea, mirnas have not been discovered until now. for genome-wide discovery of mirnas in chickpea (cicer arietinum), we sequenced the small rna content from seven major tissues/organs employing illumina technology. about 154 million reads were generated, which represented more than 20 million distinct sma ...201425151616
genome wide snp identification in chickpea for use in development of a high density genetic map and improvement of chickpea reference genome assembly.in the whole genome sequencing, genetic map provides an essential framework for accurate and efficient genome assembly and validation. the main objectives of this study were to develop a high-density genetic map using rad-seq (restriction-site associated dna sequencing) genotyping-by-sequencing (rad-seq gbs) and illumina goldengate assays, and to examine the alignment of the current map with the kabuli chickpea genome assembly.201425150411
the pgip family in soybean and three other legume species: evidence for a birth-and-death model of evolution.polygalacturonase-inhibiting proteins (pgips) are leucine-rich repeat (lrr) plant cell wall glycoproteins involved in plant immunity. they are typically encoded by gene families with a small number of gene copies whose evolutionary origin has been poorly investigated. here we report the complete characterization of the full complement of the pgip family in soybean (glycine max [l.] merr.) and the characterization of the genomic region surrounding the pgip family in four legume species.201425034494
glycolytic enzyme activities and gene expression in cicer arietinum exposed to water-deficit stress.the specific activities and transcript levels of glycolytic enzymes were examined in shoots of chickpea (cicer arietinum l.) cultivars, pusa362 (drought tolerant) and sbd377 (drought sensitive), subjected to water-deficit stress 30 days after sowing. water-deficit stress resulted in decrease in relative water content, chlorophyll content, plant dry weight, and nadp/nadph ratio and increase in nad/nadh ratio in both the cultivars. a successive decline in the specific activities of fructose-1,6-bi ...201425008554
effect of short-term cold stress on oxidative damage and transcript accumulation of defense-related genes in chickpea seedlings.cold stress affects many plant physiological and biochemical components and induces cascades of alterations in metabolic pathways, amongst them the membrane fatty acid compositions, the activity of antioxidative enzymes and the regulation of gene expression. the present work aimed to characterize the changes of some of these factors in both cold acclimated (ca) and non-acclimated (na) plants of chickpea (cicer arietinum l.) to identify the role of the acclimation process in adjusting plant respo ...201424972025
cicarmisatdb: the chickpea microsatellite database.chickpea (cicer arietinum) is a widely grown legume crop in tropical, sub-tropical and temperate regions. molecular breeding approaches seem to be essential for enhancing crop productivity in chickpea. until recently, limited numbers of molecular markers were available in the case of chickpea for use in molecular breeding. however, the recent advances in genomics facilitated the development of large scale markers especially ssrs (simple sequence repeats), the markers of choice in any breeding pr ...201424952649
kocuria flava induced growth and chromium accumulation in cicer arietinum l.in the present investigation a chromate tolerant rhizobacterium kocuria flava was isolated and inoculated to the cicer arietinum l to evaluate its effects on growth and chromium accumulation upon exposure of different concentration of chromium (1-10 microg ml(-1)) as cr (vi) for 24 d. k. flava inoculated plant of c. arietinum demonstrated luxuriant growth as compared to non inoculated plant at respective concentration of cr (vi). k. flava found to ameliorate chromium induced phytotoxicity in ter ...201424912212
organ accumulation and subcellular location of cicer arietinum st1 protein.the st (shoot specific) proteins are a new family of proteins characterized by a signal peptide, tandem repeats of 25/26 amino acids, and a domain of unknown function (duf2775), whose presence is limited to a few families of dicotyledonous plants, mainly fabaceae and asteraceae. their function remains unknown, although involvement in plant growth, fruit morphogenesis or in biotic and abiotic interactions have been suggested. this work is focused on st1, a cicer arietinum st protein. we establish ...201424908505
some synthetic cyclitol derivatives alleviate the effect of water deficit in cultivated and wild-type chickpea species.cyclitols were prepared from corresponding allylic hydroperoxides, synthesized by photooxygenation of the appropriate cyclic alkenes. these hydroperoxides were then separately treated with a catalytic amount of oso4. synthesized dl-cyclopentane-1,2,3-triol 9 (a), dl-cyclohexane-1,2,3-triol 12 (b), and dl-cycloheptane-1,2,3-triol 15 (c) were used in the investigation of plant stress. antioxidants, lipid peroxidation, and water status of chickpea species exposed to synthetic cyclitols under water ...201424877672
phylogenetic diversity of mesorhizobium in chickpea.crop domestication, in general, has reduced genetic diversity in cultivated gene pool of chickpea (cicer arietinum) as compared with wild species (c. reticulatum, c. bijugum). to explore impact of domestication on symbiosis, 10 accessions of chickpeas, including 4 accessions of c. arietinum, and 3 accessions of each of c. reticulatum and c. bijugum species, were selected and dnas were extracted from their nodules. to distinguish chickpea symbiont, preliminary sequences analysis was attempted wit ...201424845514
genetic characterization of the acetohydroxyacid synthase (ahas) gene responsible for resistance to imidazolinone in chickpea (cicer arietinum l.).a point mutation in the ahas1 gene leading to resistance to imidazolinone in chickpea was identified. the resistance is inherited as a single gene. a kasp marker targeting the mutation was developed. weed control in chickpea (cicer arietinum l.) is challenging due to poor crop competition ability and limited herbicide options. a chickpea genotype with resistance to imidazolinone (imi) herbicides has been identified, but the genetic inheritance and the mechanism were unknown. in many plant specie ...201424821525
valorization of traditional foods: nutritional and bioactive properties of cicer arietinum l. and lathyrus sativus l. pulses.the use of traditional foods can enrich our diet, perpetuating important elements of local knowledge and cultural inheritance. raw, soaked and cooked samples of two fabaceae species (cicer arietinum l. and lathyrus sativus l.) were characterized regarding nutritional and bioactive properties.201524752412
nuclear phosphoproteome of developing chickpea seedlings (cicer arietinum l.) and protein-kinase interaction network.nucleus, the control centre of eukaryotic cell, houses most of the genetic machineries required for gene expression and their regulation. post translational modifications of proteins, particularly phosphorylation control a wide variety of cellular processes but its functional connectivity, in plants, is still elusive. this study profiled the nuclear phosphoproteome of a grain legume, chickpea, to gain better understanding of such event. intact nuclei were isolated from 3-week-old seedlings using ...201424747304
morphological response of cicer arietinum exposed to paper mill effluent.the present study was conducted to assess the effect of different concentrations (control, 25, 50, 75 and 100%) of paper mill effluent on some morphological characters of cicer arietinum. result showed maximum growth promotion i.e., root length (3.8 cm), shoot length (10.0 cm) and increase in chlorophyll content (1.004 mg gm(-1)) at 50% effluent concentration after 21 days. however, at higher effluent concentration growth parameters started decreasing. the study suggests that the effluent can be ...201324640256
solid-state bioconversion of chickpea (cicer arietinum l.) by rhizopus oligosporus to improve total phenolic content, antioxidant activity and hypoglycemic functionality.the objective of this investigation was to study the effect of time during solid state bioconversion (ssb) on total phenolic content (tpc), antioxidant activity (aoxa), and inhibitory properties against α-amylase and α-glucosidase of chickpea. chickpea cotyledons were inoculated with a suspension of rhizopus oligosporus and incubated at 35 °c for 24, 36, 48, 60, 72, 84, 96 and 108 h. the best time to produce bioprocessed chickpea (added with seed coats) flour with the highest aoxa was 108 h. ssb ...201424611669
integrated physical, genetic and genome map of chickpea (cicer arietinum l.).physical map of chickpea was developed for the reference chickpea genotype (icc 4958) using bacterial artificial chromosome (bac) libraries targeting 71,094 clones (~12× coverage). high information content fingerprinting (hicf) of these clones gave high-quality fingerprinting data for 67,483 clones, and 1,174 contigs comprising 46,112 clones and 3,256 singletons were defined. in brief, 574 mb genome size was assembled in 1,174 contigs with an average of 0.49 mb per contig and 3,256 singletons re ...201424610029
androgenesis in chickpea: anther culture and expressed sequence tags derived annotation.double haploid technique is not routinely used in legume breeding programs, though recent publications report haploid plants via anther culture in chickpea (cicer arietinum l.). the focus of this study was to develop an efficient and reproducible protocol for the production of double haploids with the application of multiple stress pre-treatments such as centrifugation and osmotic shock for genotypes of interest in chickpea for their direct use in breeding programs. four genotypes, icc 4958, wr3 ...201424597152
amelioration of cd toxicity by pretreatment of salicylic acid in cicer arietinum l. seedlings.in this study, the ameliorating effect of salicylic acid (sa), serving as a mediator for protecting plants, against cadmium (cd) toxicity in cicer arietinum was investigated. the seedlings of cicer arietinum treated with increasing cd concentrations (0, 25, 50, 100 microm ) inhibited seedling length, reduced fresh and dry weight, total chlorophyll, carotenoid content and fatty acid methyl ester content. furthermore, the level of some important parameters like mda, proline and gsh content related ...201324555342
genetic behaviour of earliness related traits and seed yield in chickpea (cicer arietinum l.).genetic analysis of five quantitative traits related to earliness and seed yield in chickpea was carried out using eight segregating populations (f2 generations) and their nine parents. characters included in the study were days to flowering, flowering period, days to maturity, plant height and seed yield per plant. the results showed that no consistency in magnitude of genetic parameters was observed in any cross populations. however, the f2 of p1xp4 exhibited high magnitude of heritability cou ...201324502154
comprehensive transcriptome assembly of chickpea (cicer arietinum l.) using sanger and next generation sequencing platforms: development and applications.a comprehensive transcriptome assembly of chickpea has been developed using 134.95 million illumina single-end reads, 7.12 million single-end flx/454 reads and 139,214 sanger expressed sequence tags (ests) from >17 genotypes. this hybrid transcriptome assembly, referred to as cicer arietinumtranscriptome assembly version 2 (cata v2, available at http://data.comparative-legumes.org/transcriptomes/cicar/lista_cicar-201201), comprising 46,369 transcript assembly contigs (tacs) has an n50 length of ...201424465857
galactose content of legumes, caseinates, and some hard cheeses: implications for diet treatment of classic galactosemia.there are inconsistent reports on the lactose and/or galactose content of some foods traditionally restricted from the diet for classic galactosemia. therefore, samples of cheeses, caseinates, and canned black, pinto, kidney, and garbanzo beans were analyzed for free galactose content using hplc with refractive index or pulsed amperometric detection. galactose concentrations in several hard and aged cheeses and three mild/medium cheddars, produced by smaller local dairies, was <10 mg/100 g sampl ...201424456566
comparative assessment of issr, damd and scot markers for evaluation of genetic diversity and conservation of landrace chickpea (cicer arietinum l.) genotypes collected from north-west of iran.morphological traits and three molecular markers techniques: start codon targeted (scot), inter-simple sequence repeat (issr) and directly amplified minisatellite dna (damd) markers were compared for fingerprinting of 40 landraces chickpea genotypes collected from different geographical locations of north-west of iran. variance analysis of ten measured morphological traits showed significant differences existed between genotypes. cluster analysis based on morphological traits, divided genotypes ...201324431526
effect of soaking and cooking on dietary fibre components of different type of chickpea genotypes.processing is an important and essential component to enhance the digestibility of essential nutrients of grains. dietary fibres play an important role in bringing health advantages in chickpea and help in lowering plasma cholesterol. changes during soaking and soaking followed by cooking on cellulose, hemicellulose, lignin and pectin contents of four genotypes of desi type (kwr 108, jg 74, dcp 92-3 and bg 256), four genotypes of kabuli types (kak 2, jkg 1, bg 1053, and l 550) and two genotypes ...201324425956
genetic dissection of drought tolerance in chickpea (cicer arietinum l.).analysis of phenotypic data for 20 drought tolerance traits in 1-7 seasons at 1-5 locations together with genetic mapping data for two mapping populations provided 9 qtl clusters of which one present on calg04 has a high potential to enhance drought tolerance in chickpea improvement. chickpea (cicer arietinum l.) is the second most important grain legume cultivated by resource poor farmers in the arid and semi-arid regions of the world. drought is one of the major constraints leading up to 50% p ...201424326458
mitigation of starch-induced postprandial glycemic spikes in rats by antioxidants-rich extract of cicer arietinum linn. seeds and sprouts.consumption of highly processed calories dense diet leads abrupt increase in postprandial blood glucose level, which in turn induces immediate oxidative stress. postprandial hyperglycemia (pphg) and resultant oxidative stress is one of the earliest detectable abnormalities in diabetes prone individuals, independent risk factor for development of cardiovascular disorders (cvd), a major pathophysiological link between diabetes and cvd and an important contributing factor in atherogenesis even in n ...201324302835
mapping and identification of a cicer arietinum nsp2 gene involved in nodulation pathway.for the first time the putative nsp2 gene in chickpea has been identified using pairs of nils differing for the rn1 / rn1 nodulation gene that was located in lg5 of chickpea genetic map. an intraspecific cross between the mutant non-nodulating genotype pm233, carrying the recessive gene rn1, and the wild-type ca2139 was used to develop two pairs of near-isogenic lines (nils) for nodulation in chickpea. these pairs of nils were characterized using sequence tagged microsatellite site (stms) marker ...201424247237
differentially expressed myo-inositol monophosphatase gene (caimp) in chickpea (cicer arietinum l.) encodes a lithium-sensitive phosphatase enzyme with broad substrate specificity and improves seed germination and seedling growth under abiotic stresses.myo-inositol monophosphatase (imp) is an essential enzyme in the myo-inositol metabolic pathway where it primarily dephosphorylates myo-inositol 1-phosphate to maintain the cellular inositol pool which is important for many metabolic and signalling pathways in plants. the stress-induced increased accumulation of inositol has been reported in a few plants including chickpea; however, the role and regulation of imp is not well defined in response to stress. in this work, it has been shown that imp ...201324123252
differences between easy- and difficult-to-mill chickpea (cicer arietinum l.) genotypes. part iii: free sugar and non-starch polysaccharide composition.parts i and ii of this series of papers identified several associations between the ease of milling and the chemical compositions of different chickpea seed fractions. non-starch polysaccharides were implicated; hence, this study examines the free sugars and sugar residues.201424122880
differences between easy- and difficult-to-mill chickpea (cicer arietinum l.) genotypes. part i: broad chemical composition.ease of milling is an important quality trait for chickpeas (cicer arietinum l.) and involves two separate processes: removal of the seed coat and splitting of cotyledons. four chickpea genotypes (two desi types, one kabuli type and one interspecific hybrid with 'wild' c. echinospermum parentage) of differing ease of milling were examined to identify associated seed composition differences in the seed coat, cotyledons and their junctions (abaxial and adaxial).201424122733
differences between easy- and difficult-to-mill chickpea (cicer arietinum l.) genotypes. part ii: protein, lipid and mineral composition.part i introduced the concept of easy- and difficult-to-mill chickpea genotypes, the broad chemical composition of their seed fractions and proposed mechanistic explanations for physical differences consistent with observed variation in milling ease. part ii continues this research by delving deeper into the amino acid, fatty acid and mineral components.201424122721
identification of putative and potential cross-reactive chickpea (cicer arietinum) allergens through an in silico approach.allergy has become a key cause of morbidity worldwide. although many legumes (plants in the fabaceae family) are healthy foods, they may have a number of allergenic proteins. a number of allergens have been identified and characterized in fabaceae family, such as soybean and peanut, on the basis of biochemical and molecular biological approaches. however, our understanding of the allergens from chickpea (cicer arietinum l.), belonging to this family, is very limited.201324099701
phosphoproteomic dynamics of chickpea (cicer arietinum l.) reveals shared and distinct components of dehydration response.reversible protein phosphorylation is a ubiquitous regulatory mechanism that plays critical roles in transducing stress signals to bring about coordinated intracellular responses. to gain better understanding of dehydration response in plants, we have developed a differential phosphoproteome in a food legume, chickpea (cicer arietinum l.). three-week-old chickpea seedlings were subjected to progressive dehydration by withdrawing water, and the changes in the phosphorylation status of a large rep ...201324083463
comprehensive hormone profiling of the developing seeds of four grain legumes.developmental context and species-specific hormone requirements are of key importance in the advancement of in vitro protocols and manipulation of seed development. improvement of in vitro tissue and cell culture protocols in grain legumes such as embryo rescue, interspecific hybridization, and androgenesis requires an understanding of the types, activity, and balance of hormones within developing seeds. towards this goal, the concentration of auxin, cytokinin, gibberellin, and abscisic acid (ab ...201324062013
selenium accumulation and speciation in biofortified chickpea (cicer arietinum l.) under mediterranean conditions.millions of people have se-deficient diets and se-biofortified crops could prevent such deficiency. the aim of the present study was to evaluate the potential of chickpea for use in se fertilization programs in order to increase available se. two foliar se fertilizers (sodium selenate and sodium selenite) at four rates (0, 10, 20, 40 g ha(-1)) were tested in the 2010/2011 and 2011/2012 growing seasons in a field experiment conducted under semiarid mediterranean conditions.201423983062
purification, recovery, and characterization of chick pea (cicer arietinum) β-galactosidase in single step by three phase partitioning as a rapid and easy technique.in this study chick pea β-galactosidase was first time purified and recovered in single step by three phase partitioning (tpp). optimal purification parameters for tpp were 60% ammonium sulfate saturation (w/v) with 1:0.5 (v/v) ratio of crude extract:t-butanol at ph 6.8, which gave 10.1 purification fold with 133% recovery of β-galactosidase. sds-page analysis showed that protein has two subunits with molecular masses of 48 and 38kda, respectively. characterization of enzyme showed that optimal ...201323954616
retraction note: high-efficiency agrobacterium-mediated transformation of chickpea (cicer arietinum l.) and regeneration of insect-resistant transgenic plants. 201323907614
maize grain concentrations and above-ground shoot acquisition of micronutrients as affected by intercropping with turnip, faba bean, chickpea, and soybean.most research on micronutrients in maize has focused on maize grown as a monocrop. the aim of this study was to determine the effects of intercropping on the concentrations of micronutrients in maize grain and their acquisition via the shoot. we conducted field experiments to investigate the effects of intercropping with turnip (brassica campestris l.), faba bean (vicia faba l.), chickpea (cicer arietinum l.), and soybean (glycine max l.) on the iron (fe), manganese (mn), copper (cu) and zinc (z ...201323900569
lentils (lens culinaris l.), a rich source of folates.the potential for genetic biofortification of u.s.-grown lentils ( lens culinaris l.) with bioavailable folate has not been widely studied. the objectives of this study were (1) to determine the folate concentration of 10 commercial lentil cultivars grown in minot and mclean counties, north dakota, usa, in 2010 and 2011, (2) to determine the genotype (g) × environmental (e) interactions for folate concentration in lentil cultivars, and (3) to compare the folate concentration of other pulses [fie ...201323865478
coordinated action of β-galactosidases in the cell wall of embryonic axes during chickpea germination and seedling growth.the plant cell wall is a dynamic structure whose constant modification is necessary for plant cells to grow and divide. in the cell walls of chickpea (cicer arietinum) there are at least four β-galactosidases, whose presence and location in embryonic axes during the first 48 h of seed imbibition are discussed in this paper. we examined their roles as cell wall-modifying enzymes in germinative and/or post-germinative events. at the start of germination, only βv-gal, and to a lesser extent βiv-gal ...201423731125
macro- and microelement contents of some legume seeds.macro- and microelement contents of legume seeds were determined by icp-aes. the potassium k contents of seeds ranged between 7,426 mg/kg (lupinus albus l.) and 16,558 mg/kg (phaseolus vulgaris l. ssp. sphaericus mart). in addition, while p contents of seeds changed from 2,719 mg/kg (l. albus l.) to 5,792 mg/kg (vigna sinensis (l.) savi), ca contents ranged at the levels between 1,309 mg/kg (cicer arietinum l.) and 2,781 mg/kg (l. albus l.). the microelement contents of samples were found to be ...201323715731
sulfide ions as modulators of metal-thiolate cluster size in a plant metallothionein.metallothioneins are small cysteine-rich proteins coordinating various transition metal ions preferably with the electron configuration d(10). they are ubiquitously present in all phyla, and next to phytochelatins they represent a successful molecular concept for high-capacity metal ion binding. recent studies showed the incorporation of sulfide ions into the metal-thiolate cluster of metallothionein 2 from the plant cicer arietinum (cicmt2) increasing the cadmium binding capacity and stability ...201323636452
genotype and growing environment interaction shows a positive correlation between substrates of raffinose family oligosaccharides (rfo) biosynthesis and their accumulation in chickpea ( cicer arietinum l.) seeds.to develop genetic improvement strategies to modulate raffinose family oligosaccharides (rfo) concentration in chickpea ( cicer arietinum l.) seeds, rfo and their precursor concentrations were analyzed in 171 chickpea genotypes from diverse geographical origins. the genotypes were grown in replicated trials over two years in the field (patancheru, india) and in the greenhouse (saskatoon, canada). analysis of variance revealed a significant impact of genotype, environment, and their interaction o ...201323621405
quantitative imaging of rhizosphere ph and co2 dynamics with planar optodes.live imaging methods have become extremely important for the exploration of biological processes. in particular, non-invasive measurement techniques are key to unravelling organism-environment interactions in close-to-natural set-ups, e.g. in the highly heterogeneous and difficult-to-probe environment of plant roots: the rhizosphere. ph and co2 concentration are the main drivers of rhizosphere processes. being able to monitor these parameters at high spatio-temporal resolution is of utmost impor ...201323532048
a draft genome sequence of the pulse crop chickpea (cicer arietinum l.).cicer arietinum l. (chickpea) is the third most important food legume crop. we have generated the draft sequence of a desi-type chickpea genome using next-generation sequencing platforms, bacterial artificial chromosome end sequences and a genetic map. the 520-mb assembly covers 70% of the predicted 740-mb genome length, and more than 80% of the gene space. genome analysis predicts the presence of 27,571 genes and 210 mb as repeat elements. the gene expression analysis performed using 274 millio ...201323489434
resistance of αai-1 transgenic chickpea (cicer arietinum) and cowpea (vigna unguiculata) dry grains to bruchid beetles (coleoptera: chrysomelidae).dry grain legume seeds possessing αai-1, an α-amylase inhibitor from common bean (phaseolus vulgaris), under the control of a cotyledon-specific promoter have been shown to be highly resistant to several important bruchid pest species. one transgenic chickpea and four cowpea lines expressing αai-1, their respective controls, as well as nine conventional chickpea cultivars were assessed for their resistance to the bruchids acanthoscelides obtectus (say), callosobruchus chinensis l. and callosobru ...201323458831
effect of tio2 nanoparticles on chickpea response to cold stress.nanoparticles (nps) in agricultural systems can potentially be used as appropriate candidate for change in growth, development, productivity, and quality of plants. in the present study, we assessed the effect of tio2 np concentrations (0, 2, 5, and 10 ppm) on changes of membrane damage indexes like electrolyte leakage index (eli) and malondialdehyde (mda) during cold stress (cs) 4 °c in sensitive (ilc 533) and tolerant (sel 11439) chickpea (cicer arietinum l.) genotypes. aggregation of nps with ...201323456351
promoter of cazf, a chickpea gene that positively regulates growth and stress tolerance, is activated by an ap2-family transcription factor cap2.plants respond to different forms of stresses by inducing transcription of a common and distinct set of genes by concerted actions of a cascade of transcription regulators. we previously reported that a gene, cazf encoding a c2h2-zinc finger family protein from chickpea (cicer arietinum) imparted high salinity tolerance when expressed in tobacco plants. we report here that in addition to promoting tolerance against dehydration, salinity and high temperature, the cazf overexpressing plants exhibi ...201323418595
molecular mapping and characterization of genes governing time to flowering, seed weight, and plant height in an intraspecific genetic linkage map of chickpea (cicer arietinum).drought is the major constraint to chickpea productivity worldwide. utilizing early flowering genotypes and larger seed size have been suggested as strategies for breeding in drought zones. therefore, this study aimed to identify potential markers linked to days-to-flowering, 100-seed weight, and plant height in a chickpea intraspecific f(2:3) population derived from the cross ilc3279 × iccv2. a closely linked marker (ta117) on linkage group lg3 was identified for the days-to-flowering trait, ex ...201323371372
effect of sodium nitroprusside on morphological characters under chilling stress in chickpea (cicer arietinum l.).an experiment was conducted with chilling tolerant (ic-424234) and sensitive (pbg-1) chickpea (cicer arietinum l.) genotypes to study the effect of sodium nitroprusside (snp)- nitric oxide donor applied as foliar spray of 150 and 300 microm concentrations at 45 das (vegetative stage), 85 das (flowering stage) and 125 das (post flowering stage). both the concentrations of snp (150 and 300 microm) resulted in significant increase in all the morphological characters viz. plant height, number of lea ...201223359993
draft genome sequence of chickpea (cicer arietinum) provides a resource for trait improvement.chickpea (cicer arietinum) is the second most widely grown legume crop after soybean, accounting for a substantial proportion of human dietary nitrogen intake and playing a crucial role in food security in developing countries. we report the ∼738-mb draft whole genome shotgun sequence of cdc frontier, a kabuli chickpea variety, which contains an estimated 28,269 genes. resequencing and analysis of 90 cultivated and wild genotypes from ten countries identifies targets of both breeding-associated ...201323354103
identification of upregulated genes under cold stress in cold-tolerant chickpea using the cdna-aflp approach.low temperature injury is one of the most significant causes of crop damage worldwide. cold acclimatization processes improve the freezing tolerance of plants. to identify genes of potential importance for acclimatzation to the cold and to elucidate the pathways that regulate this process, global transcriptome expression of the chickpea (cicer arietinum l), a species of legume, was analyzed using the cdna-aflp technique. in total, we generated 4800 transcript-derived fragments (tdfs) using cdna- ...201323341906
assessment of the estrogenic activities of chickpea (cicer arietinum l) sprout isoflavone extract in ovariectomized rats.chickpea (cicer arietinum l) is a traditional uighur herb. in this study we investigated the estrogenic activities of the isoflavones extracted from chickpea sprouts (ics) in ovariectomized rats.201323334239
identification of conserved micrornas and their targets in chickpea (cicer arietinum l.).the micrornas (mirnas) are a new class of non-protein coding small rnas that regulate gene expression at the post-transcriptional level in plants. although thousands of mirnas have been identified in many plant species, little studies have been reported about chickpea micrornas. in this study, 28 potential mirna candidates belonging to 20 families were identified from 16 ests and 12 gsss in chickpea using a comparative genome-based computational analysis. a total of 664 mirna targets were predic ...201323333975
molecular characterization of primary gene pool of chickpea based on issr markers.genetic diversity and relationships within and among members of the primary gene pool of chickpea, including 38 accessions of cicer arietinum, six of c. reticulatum,, and four of c. echinospermum, were investigated using 31 issr markers. the study revealed moderate diversity, detecting 141 fragments, of which 79 (56%) were polymorphic. averages were 0.125 for polymorphic information content, 0.350 for marker index, and 0.715 for resolving power. the upgma dendrogram and the principal coordinate ...201323329257
comparative analysis of kabuli chickpea transcriptome with desi and wild chickpea provides a rich resource for development of functional markers.chickpea (cicer arietinum l.) is an important crop legume plant with high nutritional value. the transcriptomes of desi and wild chickpea have already been sequenced. in this study, we sequenced the transcriptome of kabuli chickpea, c. arietinum (genotype iccv2), having higher commercial value, using gs-flx roche 454 and illumina technologies. the assemblies of both roche 454 and illumina datasets were optimized using various assembly programs and parameters. the final optimized hybrid assembly ...201223300670
multi-environment qtl analyses for drought-related traits in a recombinant inbred population of chickpea (cicer arietinum l.).a recombinant inbred line (ril) population, comprising 181 lines derived from ilc588 × ilc3279, was evaluated in 10 environments across three locations with different moisture gradients. a drought resistance score (drs) and three phenology traits-plant height (plht), days to flowering (dflr), and days to maturity (mat)-were recorded along with seven yield-related traits-grain yield (gy), biological yield (by), harvest index (hi), the number of pods/3 plants (pod), percentage of empty pods (%epod ...201323283512
trichoderma inoculation ameliorates arsenic induced phytotoxic changes in gene expression and stem anatomy of chickpea (cicer arietinum).arsenic, a carcinogenic metalloid severely affects plant growth in contaminated areas. present study shows role of trichoderma reesei nbri 0716 (nbri 0716) in ameliorating arsenic (as) stress on chickpea under greenhouse conditions. arsenic stress adversely affected seed germination (25%), chlorophyll content (44%) and almost eliminated nodule formation that were significantly restored on nbri 0716 inoculation. it also restored stem anomalies like reduced trichome turgidity and density, deformat ...201323273619
incorporation of sulfide ions into the cadmium(ii) thiolate cluster of cicer arietinum metallothionein2.the plant metallothionein2 from cicer arietinum (chickpea), cic-mt2, is known to coordinate five divalent metal ions such as zn(ii) or cd(ii), which are arranged in a single metal thiolate cluster. when the zn(ii) form of the protein is titrated with cd(ii) ions in the presence of sulfide ions, an increased cd(ii) binding capacity and concomitant incorporation of sulfide ions into the cluster are observed. the exact stoichiometry of this novel cluster, its spectroscopic properties, and the signi ...201323272701
optimization of an efficient protein extraction protocol compatible with two-dimensional electrophoresis and mass spectrometry from recalcitrant phenolic rich roots of chickpea (cicer arietinum l.).two-dimensional electrophoresis and mass spectrometry are undoubtedly two essential tools popularly used in proteomic analyses. utilization of these techniques however largely depends on efficient and optimized sample preparation, regarded as one of the most crucial steps for recovering maximum amount of reliable information. the present study highlights the optimization of an effective and efficient protocol, capable of extraction of root proteins from recalcitrant phenolic rich tissues of chic ...201223193474
transcriptomic analysis of oxylipin biosynthesis genes and chemical profiling reveal an early induction of jasmonates in chickpea roots under drought stress.drought is one of the major constraints in subtropical agriculture. therefore improving water stress tolerance is of great importance to breed for drought tolerance in future. the first plant organ sensing dehydration is the root. aim of the present work was to clarify the potential impact of the phyto-oxylipins pathway on drought tolerance of chickpea (cicer arietinum), the third important legume crop worldwide. therefore, we measured the expression of key genes involved in oxylipins metabolism ...201223141673
a β-galactosidase from chick pea (cicer arietinum) seeds: its purification, biochemical properties and industrial applications.a β-galactosidase from cicer arietinum seeds has been purified to apparent electrophoretic homogeneity using a combination of various fractionation and chromatographic techniques, giving a final specific activity of 220 units mg(-1), with approximately 1840 fold purification. analysis of the protein by sds-page revealed two subunits with molecular masses of 48 and 38 kda, respectively. these bands were further confirmed with lc-ms/ms, indicating that chick pea β-galactosidase (cpgal) is a hetero ...201223107735
water soluble carbon nano-onions from wood wool as growth promoters for gram plants.water-soluble carbon nano-onions (wscnos) isolated from wood wool-a wood-based pyrolysis waste product of wood, can enhance the overall growth rate of gram (cicer arietinum) plants. treatment of plants with upto 30 μg ml(-1) of wscnos for an initial 10 day period in laboratory conditions led to an increase in the overall growth of the plant biomass. in order to examine the growth stimulating effects of wscnos under natural conditions, 10 day-old plants treated with and without wscnos were transp ...201223099536
inheritance of natural seed-coat cracking in chickpea.a spontaneous mutant with natural seed-coat cracking, designated "cracked seed-coat mutant (csm)," was identified in chickpea (cicer arietinum l.) from an f(2) population of a cross icrisat chickpea (icc) 10301 × icc 12430. the extent of seed-coat cracking (scc) varied widely from a minute to several wide cracks. seed coats showed cracks before seeds were fully developed and the plants had reached physiological maturity. however, seed-coat cracks were most visible on dry matured seeds, particula ...201223077231
the estrogenic activity of isoflavones extracted from chickpea cicer arietinum l sprouts in vitro.isoflavones have drawn attention due to their potential therapeutic use. isoflavones are the important chemical components of the seeds and sprouts of chickpea and higher isoflavones in sprouts than in seeds. however, there have been no previous reports of the estrogenic activity of isoflavones extracted from chickpea cicer arietinum l sprouts (ics) in vitro. in this study, which incorporated several in vitro bioassays methods, we systematically evaluated the estrogenic properties of ics. mtt as ...201323065723
change in membrane fatty acid compositions and cold-induced responses in chickpea.plant cells often increase cold tolerance by reprogramming their genes expression which results in adjusted metabolic alternations, a process enhanced under cold acclimation (ca) phase. in present study, we assessed the changes of membrane fatty acid compositions and defense machine (like antioxidative enzymes) along with damage indexes like electrolyte leakage index (eli) and malondialdehyde (mda) during ca, cold stress (cs) and recovery (r) phases in chickpea (cicer arietinum l.). results show ...201323065233
ectopic expression of the aba-inducible dehydration-responsive chickpea l-myo-inositol 1-phosphate synthase 2 (camips2) in arabidopsis enhances tolerance to salinity and dehydration stress.myo-inositol participates in many different aspects of plant physiology and myo-inositol 1-phosphate synthase (mips; ec 5.5.1.4) catalyzes the rate limiting step of inositol biosynthetic pathway. chickpea (cicer arietinum), a drought-tolerant leguminous crop plant, is known to accumulate increased inositol during dehydration stress. previously, we reported two differentially expressed divergent genes (camips1 and camips2) encoding two mips isoforms in chickpea. in this communication, we demonstr ...201323065054
proteomic analysis reveals the diversity and complexity of membrane proteins in chickpea (cicer arietinum l.). 201223031650
dissipation of pendimethalin in soil and its residues in chickpea (cicer arietinum l.) under field conditions.disappearance of pendimethalin in the soil of chickpea (cicer arietinum l.) at 0-110 days, and terminal residues in plant samples have been studied under field conditions. pendimethalin was applied as pre-emergence herbicide at 750, 350 and 180 g a.i. ha(-1) in winter, in chickpea crop. the dissipation of pendimethalin in the chickpea field soil conditions followed first order kinetics showing a half-life of 11.23 days averaged over all doses. low pendimethalin residues were found in plant sampl ...201222983723
phenetic and functional characterization of endophytic root-nodule bacteria isolated from chickpea (cicer arietinum l.) and mothbean (vigna aconitifolia l.) of arid-and semi-arid regions of rajasthan, india.in the present study we recovered endophytic root-nodule bacteria from chickpea (cicer arietinumi l.) and mothbean (vigna aconitifolia l.). phenotypic and genotypic characterization of isolates was performed by employing biochemical and genetic approaches. sequencing data showed that most isolates belonged to genus, pseudomonas spp. being a dominant species. they also showed similarity with rhizobium, agrobacterium and erwinia spp. isolates were screened functionally for indole-3-acetic acid, si ...201224205759
a liquid chromatography method for determination of selected amino acids, coenzymes, growth regulators, and vitamins from cicer arietinum (l.) and solanum lycopersicum (l.).a bottleneck in crosstalk and qc research has been the quantification of diverse chemotypes in small amounts of tissue. an lc-uv method for estimating 28 selected metabolites of the regulatory network underlying growth, development, maintenance, vital functions, defense reactions, and food quality is reported. the method was based on binary gradient resolutions of the analytes in an rp c18 column. the mobile phase comprised solvent a [water+0.1% trifluoroacetic acid (tfa)] and b (acetonitrile + ...201322970584
allelopathic effect of ashwagandha against the germination and radicle growth of cicer arietinum and triticum aestivum.ashwagandha (withania somnifera) is an important medicinal plant in indian traditional system of medicine and traditionally has been used for several medicinal purposes in the indian subcontinent.201222923955
nutritional quality and health benefits of chickpea (cicer arietinum l.): a review.chickpea (cicer arietinum l.) is an important pulse crop grown and consumed all over the world, especially in the afro-asian countries. it is a good source of carbohydrates and protein, and protein quality is considered to be better than other pulses. chickpea has significant amounts of all the essential amino acids except sulphur-containing amino acids, which can be complemented by adding cereals to the daily diet. starch is the major storage carbohydrate followed by dietary fibre, oligosacchar ...201222916806
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