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molecular cloning, characterisation and expression of a manganese superoxide dismutase gene from peach (prunus persica [l.] batsch).two cdna clones encoding mitochondrial manganese superoxide dismutases (mnsods) from peach ( prunus persica [l.] batsch) were identified, which show homologies to several plant mnsods. the amino acid sequence predicted from one full-length clone ( mnsod1) showed the highest homology to an mnsod from nicotiana plumbaginifolia (94%) and included a 24-amino acid transit peptide typical of those used to target proteins to the mitochondria. a second, partial clone ( mnsod2) showed divergence from mns ...200212073034
peach (prunus persica) fruit ripening: aminoethoxyvinylglycine (avg) and exogenous polyamines affect ethylene emission and flesh firmness.the effect of various concentrations of aminoethoxyvinylglycine (avg; 0.32 and 1.28 mm), an ethylene biosynthesis inhibitor, and of the polyamines putrescine (10 mm), spermidine (0.1, 1 and 5 mm) and spermine (2 mm) on peach (prunus persica l. batsch cv. redhaven) fruit ripening was evaluated under field conditions. treatments were performed 19 (polyamines) and 8 (avg) days before harvest. fruit growth (diameter, fresh and dry weight), flesh firmness, soluble solids content and ethylene emission ...200212060270
distinct nuclear organization, dna methylation pattern and cytokinin distribution mark juvenile, juvenile-like and adult vegetative apical meristems in peach (prunus persica (l.) batsch).chromatin organization, nuclear dna methylation and endogenous zeatin localization were investigated in shoot apical meristems (sam) during juvenile and adult phases of peach (prunus persica (l.) batsch). the aim was to examine the extent to which these parameters could discriminate the juvenile and adult sams. seedlings (juvenile, cannot flower), basal shoots (called juvenile-like, because they exhibit juvenile macroscopic traits) and apical shoots (competent to form flowers) of adult plants we ...200211971916
isolation and characterization of six peach cdnas encoding key proteins in organic acid metabolism and solute accumulation: involvement in regulating peach fruit acidity.as in many other fleshy fruits, the predominant organic acids in ripe peach (prunus persica (l.) batsch) fruit are malic and citric acids. the accumulation of these metabolites in fruit flesh is regulated during fruit development. six peach fruit-related genes implicated in organic acid metabolism (mitochondrial citrate synthase; cytosolic nad-dependent malate dehydrogenase, and cytosolic nadp-dependent isocitrate dehydrogenase) and storage (vacuolar proton translocating pumps: one vacuolar h+-a ...200211903973
ethylene-responsive genes are differentially regulated during abscission, organ senescence and wounding in peach (prunus persica).ethylene-responsive genes from peach (prunus persica, l. batsch) were isolated by differential screening of a cdna library constructed from abscission zones in which cell separation had been evoked by treatment with the ethylene analogue propylene. dna and deduced protein sequences of four selected clones, termed prunus persica abscission zone (ppaz), revealed homology to thaumatin-like proteins (ppaz8 and ppaz44), to proteins belonging to the pr4 class of pathogenesis-related (pr) proteins (ppa ...200211847241
changes in the risk of fine-root mortality with age: a case study in peach, prunus persica (rosaceae).previous studies suggest that younger roots are more vulnerable to mortality than older roots. we analyzed minirhizotron data using a mixed-age, proportional hazards regression approach to determine whether the risk of mortality (or "hazard") was higher for younger roots than for older roots in a west virginia peach orchard. while root age apparently had a strong effect on the hazard when considered alone, this effect was largely due to different rates of mortality among roots of different order ...200221669714
theoretical analysis of systematic errors introduced by a pedicel-girdling technique used to estimate separately the xylem and phloem flows.the water budget of fruits was analysed by means of a biophysical model of fruit growth, built and calibrated recently for peaches [prunus persica (l.) batsch]. this analysis was applied to the evaluation of systematic errors introduced by a pedicel-girdling method (with the observations being treated by means of a subtractive technique) used to separate the contributions of xylem and phloem flow to the total water inflow to the fruit. the flows were considered as solution transport through comp ...200111735290
a peach linkage map integrating rflps, ssrs, rapds, and morphological markers.a linkage map was obtained using a bc1 progeny (prunus persica x (p. persica x p ferganensis)). the map is composed of 109 loci (74 rflps, 17 ssrs, 16 rapds, and two morphological traits) distributed in 10 linkage groups. loci, segregating in five different ratios, were integrated in the map with joinmap 2.0 software. the map covers 521 cm of the peach genome. the average distance between adjacent loci is 4.8 cm. two monogenic traits, flesh adhesion (f/f) and leaf glands (e/e), were placed on th ...200111681601
changes in fine root production and longevity in relation to water and nutrient availability in a norway spruce stand in northern sweden.the peach computer simulation model of reproductive and vegetative growth of peach trees (grossman and dejong 1994) was adapted to estimate seasonal nitrogen (n) dynamics in organs of mature peach (prunus persica (l.) batsch cv. o'henry) trees grown with high and low soil n availability. seasonal n accumulation patterns of fruits, leaves, stems, branches, trunk and roots of mature, cropping peach trees were modeled by combining model predictions of organ dry mass accumulation from the peach mode ...200111581020
differential expression of four members of the h+-atpase gene family during dormancy of vegetative buds of peach trees.vegetative-bud dormancy in peach (prunus persica l. batsch) trees is known to be correlated, at least partially, with properties of the underlying bud tissues during winter. variations in the activity and amount of plasma-membrane h -atpase were observed. a full-length cdna, ppa2 (prunus persica h+-atpase 2) and three partial cdnas (ppa1, ppa3 and ppa4) for the plasma-membrane h+-atpase from peach trees were isolated by reverse transcription (rt)-coupled rapid amplification of cdna ends (race) p ...200111525520
variation in surface conductance to water vapor diffusion in peach fruit and its effects on fruit growth assessed by a simulation model.surface conductance to water vapor diffusion was measured in individual peach fruits (prunus persica (l.) batsch) and plotted as a function of fresh fruit mass for four cultivars. surface conductance increased with fresh fruit mass, but the pattern differed with cultivar, and fruit-to-fruit variation occurred. relationships between fruit mass and surface conductance were modeled by fitting mathematical equations to the data. the simulation model of fishman and génard (1998) was used to study dry ...200111470659
endogenous gibberellins in immature seeds of prunus persica l.: identification of ga(118), ga(119), ga(120), ga(121), ga(122) and ga(126).the endogenous gibberellins in immature seeds of prunus persica were analyzed by gas chromatography-mass spectrometry. eleven known gibberellins, ga(3), ga(9), ga(17), ga(19), ga(30), ga(44), ga(61), ga(63), ga(87), ga(95) and ga(97) were identified. additionally, several hitherto unknown gibberellins were detected and their putative structures were verified by synthesis of the authentic gibberellins. these gibberellins were then assigned trivial numbers, e.g. 1alpha-hydroxy ga(20) (ga(118)), 1a ...200111397444
effects of timing of nitrogen fertilization on shoot development in peach (prunus persica) trees.shoot development was studied for two consecutive years in peach trees fertilized with n either in the previous fall or in the middle of the growing season. during the first year, two additional treatments were studied: no n supply and nitrate supplied in the irrigation water throughout the growing season. the number of shoots that developed depended on nitrogen availability in the period following bud break. during shoot development, leaf emergence occurred in one, two, or three stages, which e ...200111260822
pectin esterase, polygalacturonase and gel formation in peach pectin fractions.peaches (prunus persica cv. hermoza) were stored at 0c in regular air (ra) or in controlled atmosphere (ca 10% co2, 3% o2) for 4 weeks and then ripened for 4 days at 20 degrees c. woolliness developed in the regular air stored fruit while the controlled atmosphere stored fruit ripened normally. in the woolly fruit symptoms of the disorder were greater in the inner mesocarp than in the outer. polygalacturonase (pg) and pectin esterase (pe) activities differed in the outer and inner mesocarp of th ...200011142841
mapping meristem respiration of prunus persica (l.) batsch seedlings: potential respiration of the meristems, o2 diffusional constraints and combined effects on root growth.root system architecture partially results from meristem activities, which themselves depend on endogenous and environmental factors, such as o2 depletion. in this study, meristem respiration and growth was measured in the root systems of three prunus persica (l.) batsch seedlings. the spatial distribution of meristem respiration within the root system was described, and the relationship between the respiration rates and meristem radii was analysed, using a model of radial o2 diffusion and consu ...200010938868
microsatellite dna in peach (prunus persica l. batsch) and its use in fingerprinting and testing the genetic origin of cultivars.we isolated and sequenced 26 microsatellites from two genomic libraries of peach cultivar 'redhaven', enriched for ac/gt and ag/ct repeats, respectively. for 17 of these microsatellites, it was possible to demonstrate mendelian inheritance. microsatellite polymorphism was assayed in 50 peach and nectarine cultivars. of the 1300 pcrs carried out, all but two produced amplified products of the expected size. all microsatellites were polymorphic, showing 2-8 alleles per locus. heterozygosity ranged ...200010902716
isolation and characterization of a 60 kda 2,4-d-binding protein from the shoot apices of peach trees (prunus persica l.); it is a homologue of protein disulfide isomerase.to obtain a candidate auxin-binding protein (abp), a soluble 60 kda protein was isolated from an extract of shoot apices of peach trees (prunus persica l.) by affinity chromatography on a 2,4-dichlorophenoxyacetic acid (2,4-d)-linked sepharose4b column. the 60 kda polypeptide, designated pp60, was purified as a single band on sds-page by column chromatography. its dissociation constant (kd) for [14c]-2,4-d was calculated to be 3.5 x 10(-5) m. the binding of pp60 for [14c]-2,4-d was inhibited by ...200010845464
isolation and molecular characterisation of the gene encoding the cytoplasmic ribosomal protein s28 in prunus persica [l.]] batsch.rt-pcr was performed on peach (prunus persica [l.] batsch) rna to isolate cdnas corresponding to transcripts which are differentially expressed in leaves borne on basal and apical shoots. a gene was identified which was more highly expressed in the leaves of basal shoots, and codes for the cytoplasmic protein s28 present in the small ribosomal subunit. the 5' leader regions of rps28 mrnas were found to harbour 8-11 pyrimidine tracts, which suggested similarities to regulatory stretches that cont ...200010778738
technological processes to decrease the allergenicity of peach juice and nectar.among vegetable foods peach (prunus persica) has been recognized as a significant cause of allergy. the protein, which is considered to be the major peach allergen, has been named pru p 1. because peaches are consumed both as fresh fruits and after processing to obtain peach juice, nectar, jam, syrupy peach, etc., research was carried out to identify a technological process for production of hypo- or nonallergenic peach-based products. sds-page and immunoblotting analysis of extracts prepared fr ...200010691663
complete amino acid sequence determination of the major allergen of peach (prunus persica) pru p 1.the major protein allergen of peach (prunus persica), pru p 1, has recently been identified as a lipid transfer protein (ltp). the complete primary structure of pru p 1, obtained by direct amino acid sequence and liquid chromatography-mass spectrometry (lc-ms) analyses with the purified protein, is described here. the protein consists of 91 amino acids with a calculated molecular mass of 9178 da. the amino acid sequence contains eight strictly conserved cysteines, as do all known ltps, but secon ...199910614824
differences in key odorants of handmade juice of yellow-flesh peaches (prunus persica l.) induced by the workup procedure.application of aroma extract dilution analysis (aeda) on a flavor extract isolated from a freshly prepared, enzyme-inactivated peach juice using solvent extraction and high-vacuum distillation (extract i) revealed 24 odor-active regions in the gas chromatogram. flavor dilution (fd) factors ranged from 4 to 512. the highest fd factors were determined for beta-damascenone (cooked-apple-like) and gamma-decalactone (peach-like). cooking of peaches for 2 h in an apparatus equipped for simultaneous st ...199910552883
crop load and water stress effects on daily stem growth in peach (prunus persica).we investigated crop load and water stress effects on diurnal stem extension growth of field-grown peach (prunus persica (l.) batsch) trees. neither the presence of fruit nor reduced irrigation significantly altered the timing of diurnal fluctuations in stem growth rate. stems with subtending fruit had significantly reduced growth compared to stems with no subtending fruit. crop load had no significant effect on relative stem extension rates and the majority of the reduction in absolute growth w ...199714759839
seasonal expression of a dehydrin gene in sibling deciduous and evergreen genotypes of peach (prunus persica [l.] batsch).a cdna library was created from cold-acclimated bark tissue of peach and selectively probed using an antibody directed against the lysine-rich consensus region of dehydrin proteins. several clones were thus obtained which had a high degree of sequence similarity to other dehydrin genes. northern analysis, using clone 5a, indicated that a 1.8 kb transcript was seasonally expressed in sibling deciduous and evergreen genotypes of peach, and also inducible by water deficit in cv. rio oso gem. the ev ...19979037159
analysis of molecular markers associated with powdery mildew resistance genes in peach (prunus persica (l.) batsch)xprunus davidiana hybrids.a progeny of 77 hybrids issued from a cross between two heterozygous prunus, peach [p. persica (l.) batsch] (variety 'summergrand') and a related species, p. davidiana (clone 1908), was analysed for powdery mildew resistance in five independent experiments. this population was also analysed for its genotype with isoenzyme and rapd markers in order to map the genes responsible for resistance. a genetic linkage map was generated for each parent. the 'summergrand' linkage map is composed of only fo ...199624162425
water stress and crop load effects on fruit fresh and dry weights in peach (prunus persica).effects of water stress on fruit fresh and dry weights were investigated in peach trees, prunus persica (l.) batsch., with varying crop loads: light, moderate and heavy. in well-watered controls, tree water status was independent of crop load. in trees receiving reduced irrigation, the degree of water stress increased with increasing crop load. water stress induced fruit fresh weight reductions at all crop loads. fruit dry weight was not reduced by water stress in trees having light to moderate ...199614871677
[protein analysis of 6 crude drugs and their processed products by polyacrylamide gel electrophoresis technique].in this paper, the proteins in 6 crude drugs (prunus persica; p. armeniaca; dolichos lablab; strychnos nux-vomica; mylabris phalerata; whitmania pigra) and their processed products were analysed by polyacrylamide gel electrophoresis technique, and the effect of different processing methods on the quantity and kind of protein was explored. protein electrophorograms of 20 samples are drawn.19958679088
genetic linkage mapping in peach using morphological, rflp and rapd markers.we have constructed a genetic linkage map of peach [prunus persica (l.) batsch] consisting of rflp, rapd and morphological markers, based on 71 f2 individuals derived from the self-fertilization of four f1 individuals of a cross between 'new jersey pillar' and kv 77119. this progeny, designated as the west virginia (wv) family, segregates for genes controlling canopy shape, fruit flesh color, and flower petal color, size and number. the segregation of 65 markers, comprising 46 rflp loci, 12 rapd ...199524173944
ethylene enhances shoot formation in cultures of the peach rootstock gf-677 (prunus persica x p. amygdalus).the influence of ethylene on shoot formation from gf-677 (prunus persica × p. amygdalus) shoot tip explants was studied in vitro. cultures in test tubes were placed inside 5 1 glass jars and supplemented with various ethylene concentrations (0-10 ppm). ethylene at 0.1 ppm, applied during the first 2 weeks of culture, increased the number and the length of shoots produced in vitro. test tubes with cultures sealed with various types of closure accumulated in their atmosphere different levels of et ...199524185661
purification of three pectin esterases from ripe peach fruit.three isoforms of pectin esterase (pe1-pe3) (pectin pectyl-hydrolase, ec 3.1.1.11.) were purified to homogeneity from ripe peach fruit (prunus persica cv. coronet). the three enzymes were basic proteins of m(r) 34,000 as determined by denaturing polyacrylamide gel electrophoresis but were separated by fplc cation-exchange chromatography. the proteins were n-terminally blocked but amino acid sequences were obtained for peptides released from two of the three isoforms. the sequences revealed a thr ...19947765664
modeling carbon export out of mature peach leaves.the characteristics of sorbitol and sucrose export out of mature leaves in seedlings of peach (prunus persica l. batsch cv gf 305) were investigated by simulating carbon fluxes through the leaf. three treatments were employed: a control treatment and two treatments modifying leaf export, the latter using either shading or girdling. photosynthesis and 14c partitioning into sorbitol and sucrose were measured during carbohydrate pool buildup at the beginning of the photoperiod, and the export rate ...199412232352
ripening of nectarine fruit (changes in the cell wall, vacuole, and membranes detected using electrical impedance measurements).electrical impedance measurements were used to characterize changes in intracellular and extracellular resistance as well as changes in the condition of membranes during ripening of nectarines (prunus persica [l.] batsch cv fantasia). these measurements were related to changes in fruit texture assessed by flesh firmness and apparent juice content. an electrical model indicated that, during ripening (d 1-5) of freshly harvested fruit, the resistance of the cell wall and vacuole declined by 60 and ...199412232315
cold acclimation in genetically related (sibling) deciduous and evergreen peach (prunus persica [l.] batsch). ii. a 60-kilodalton bark protein in cold-acclimated tissues of peach is heat stable and related to the dehydrin family of proteins.in several plant species, certain cold-regulated proteins share unique properties. these proteins are (a) heat stable and (b) hydrophilic and are related to the group 2 late embryogenesis abundant or dehydrin family of proteins. our previous work with sibling deciduous and evergreen peach genotypes demonstrated a correlation between the level of accumulation of certain bark proteins and cold-acclimation potential of these tissues. here we identify a 60-kd bark protein in peach (prunus persica [l ...19948029367
peach (prunus persica) endopolygalacturonase cdna isolation and mrna analysis in melting and nonmelting peach cultivars.two distinct partial cdnas, prf1 and prf3, similar in sequence to previously described polygalacturonases, were amplified from ripe peach (prunus persica l. batsch cv flavorcrest) fruit cdna by the polymerase chain reaction. prf1-related rna was present in fruit from early ripening at levels not detected by northern analysis. prf3-related rna was readily detectable in ripe fruit by northern analysis. prf3 was used to isolate a cdna with a complete open reading frame, prf5, from a lambda zap ii c ...19948029352
targeted mapping and linkage analysis of morphological isozyme, and rapd markers in peach.nine different f2 families of peach [prunus persica (l.) batsch] were analyzed for linkage relationships between 14 morphological and two isozyme loci. linkage was detected between weeping (we) and white flower (w), 33 cm; double flower (dl) and pillar (br), 10 cm; and flesh color (y) and malate dehydrogenase (mdh1), 26 cm. a leaf variant phenotypically distinct from the previously reported wavy-leaf (wa) mutant in peach was found in progeny of 'davie ii'. the new willow-leaf character (designat ...199424190466
carbon fluxes in mature peach leaves.the turnover and transport of sugars are described in peach (prunus persica l. batsch), a species exporting both sucrose and sorbitol. apparent export rate was slower in peach leaves than in leaves of herbaceous species. sorbitol was the major soluble end product of photosynthesis and the major soluble carbohydrate in the leaf (higher than sucrose). carbon fluxes were described using (14)c labeling, radioactivity loss curves, and compartmental analysis during the second half of the photoperiod w ...199216653212
cell wall changes in nectarines (prunus persica) : solubilization and depolymerization of pectic and neutral polymers during ripening and in mealy fruit.nectarine fruit (prunus persica l. batsch var nectarina [ait] maxim) cultivar fantasia were either ripened immediately after harvest at 20 degrees c or stored for 5 weeks at 2 degrees c prior to ripening. fruit ripened after 5 weeks of storage did not soften to the same extent as normally ripened fruit, they lacked juice, and had a dry, mealy texture. pectic and hemicellulosic polysaccharides were solubilized from the mesocarp of the fruit using phenol:acetic acid:water (paw) treatment to yield ...199216653106
comparison of pch313 (ptom13 homolog) rna accumulation during fruit softening and wounding of two phenotypically different peach cultivars.pch313 was isolated as a cdna whose rna accumulated during the softening period of peach (prunus persica l. batsch) fruit development. to better understand the role of the gene, we compared the amount of pch313-related rna detected during fruit softening and tissue wounding between cultivars with different softening characteristics. the cultivar that softened faster, "bailey," had a significantly higher amount of pch313-related rna accumulate during softening than the slower-softening cultivar, ...199216652987
cold acclimation in genetically related (sibling) deciduous and evergreen peach (prunus persica [l.] batsch): i. seasonal changes in cold hardiness and polypeptides of bark and xylem tissues.seasonal patterns of proteins and of cold hardiness were characterized in bark and xylem tissues of genetically related (sibling) deciduous and evergreen peach (prunus persica [l.] batsch). in contrast with deciduous trees, which entered endodormancy and abscised leaves in the fall, evergreen trees retained their leaves and exhibited shoot elongation under favorable environmental conditions. a successive increase in the cold hardiness of bark and xylem was observed during the fall in both genoty ...199216669074
food allergy: identification of the major ige-binding component of peach (prunus persica).since peaches are a relatively common cause of food allergy, we set out to identify the allergens involved. with the use of a panel of 48 sera from patients allergic to peach, we demonstrate that most of the allergenicity of that fruit is confined to the skin, rather than to the flesh of peaches, and corresponds to a protein doublet with an estimated molecular weight of 8-10 kd.19921380782
level of abscisic acid in integuments, nucellus, endosperm, and embryo of peach seeds (prunus persica l. cv springcrest) during development.free abscisic acid (aba) in integuments, nucellus, endosperm, and embryo was determined throughout seed development of peach (prunus persica l. cv springcrest). quantification of aba was performed using combined high performance liquid chromatography-radioimmunoassay based on a monoclonal antibody raised against free (s)-aba. in the integuments and endosperm, aba concentration remained constant during the first 100 days after anthesis and rose in the following days when fresh weight was rapidly ...199116668468
effect of macerase, oxalic acid, and egta on deep supercooling and pit membrane structure of xylem parenchyma of peach.the object of this study was to determine if calcium cross-linking of pectin in the pit membrane of xylem parenchyma restricts water movement which results in deep supercooling. current year shoots of ;loring' peach (prunus persica) were infiltrated with oxalic acid or egta solutions for 24 or 48 hours and then either prepared for ultrastructural analysis or subjected to differential thermal analysis. the effect of 0.25 to 1.0% pectinase (weight/volume) on deep supercooling was also investigated ...199116668341
characterization of peroxidases in lignifying peach fruit endocarp.developing peach (prunus persica l. batsch ;redskin') fruit were used to characterize the role of peroxidases in lignification. during development, the endocarp of these drupes becomes lignified while the mesocarp remains parenchymatous. acidic peroxidase from lignifying endocarp were similar to those of the fleshy mesocarp. the endocarp had a larger amount and number of basic peroxidases than the mesocarp. cultured peach leaf cells are thought to be lignified because their walls give a positive ...199116667963
distribution, frequency, and population density of nematodes in west virginia peach orchards.nematode population densities were determined in soil and root samples collected from 205 peach (prunus persica l.) orchard blocks between 25 march and 5 may 1986. representative specimens from 75 blocks were identified to species; 28 species of plant-parasitic nematodes were identified. predaceous nematodes (mononchidae) were observed in 71% of the samples. the most common plant-parasitic genera were paratylenchus, helicotylenchus, pratylenchus, and xiphinema, occurring in 85, 84, 77, and 74% o ...199019287785
rapid multiplication of adventitious somatic embryos in peach and nectarine by secondary embryogenesis.somatic embryos were multiplied by secondary embryogenesis in cotyledonary cultures of peach and nectarine (prunus persica l.) using a simplified culture medium for immature seeds. a three-stage process with an initial callus phase was established in darkness on a medium containing basal salts (modified ms) supplemented with 2,4-d (5 mg/l), kn (2 mg/l) and bap (2 mg/l) and casein hydrolysate (500 mg/l). this was followed by a growth regulator-free medium with activated charcoal for the adventiti ...199024226827
amino acids as determinants of host preference for the xylem feeding leafhopper, homalodisca coagulata (homoptera: cicadellidae).homalodisca coagulata is a highly polyphagous xylem feeder with distinct seasonal patterns in it's selection of host plants. these patterns were examined in relation to the amino acid content of the xylem for four common host species; lagerstroemia indica, baccharis halimifolia, prunus persica, and prunus salicina. xylem fluid was collected from each host species at times when numbers of feeding leafhoppers were both low and high. in each case, concentrations of amino acids were greatest when nu ...199028313004
an experimental test of a nitrogen uptake and partitioning model for young trees.simulation models of nitrate uptake and total nitrogen partitioning during the exponential growth phase of one-year-old peach trees (prunus persica (l.) batsch.) were tested in an experiment with 88 plants grown in soil-filled containers. plants were fertilized with (15)n-no(3) (-) and nitrate uptake estimated by periodic destructive analysis of plants for excess (15)n. partitioning of n within the trees was followed by the analysis of plant parts for total n and (15)n. the nitrate uptake model, ...198914972966
carbohydrate requirements of peach fruit growth and respiration.data on the seasonal patterns of fruit growth and dark respiration of two peach (prunus persica (l.) batsch) cultivars were combined with temperature data to calculate the carbohydrate requirements of an "average" peach fruit from bloom to harvest. the two peach cultivars used were june lady (an early maturing (mid-june) cultivar) and o'henry (a late maturing (early-august) cultivar). at harvest, the mean dry weight of the june lady fruit was 17.8 g (139.7 g fresh weight) and of o'henry fruits w ...198914972978
supercooling characteristics of isolated peach flower bud primordia.the amount of unfrozen water in dormant peach (prunus persica [l.] batsch, cv redhaven) flower buds, isolated primordia, and bud axes was determined during freezing using pulse nuclear magnetic resonance methods. differential thermal analysis studies were conducted on whole buds and isolated primordia in the presence of ice nucleation. the results showed that some of the water in isolated primordia remained supercooled in the presence of ice nucleation. although most tissue water froze (57.5%) f ...198916666658
diagnosis of allergy to peach. a comparative study of "in vivo" and "in vitro" techniques.in our particular mediterranean region, extensive plantations of peach trees (prunus persica) give rise to processes of peach hypersensitivity. this datum together with the scarcity of literature and the use of disparate methods of diagnosis prompted us to select the peach as an example of hypersensitivity to a fruit, and to examine the clinical symptoms, skin tests, specific ige antibodies (rastr) and histamine release in a group of 25 patients with peach allergy. the results showed a good conc ...20062459947
measurement of indole-3-acetic acid in peach fruits (prunus persica l. batsch cv redhaven) during development.the amount of indole-3-acetic acid (iaa) was measured in peach fruits by gas chromatography-mass spectrometry-selective ion monitoring using an isotope dilution assay with [(13)c(6)]iaa as an internal standard throughout the growing season. ethylene evolution of the fruit was also measured. iaa levels were 25 nanograms per gram fresh weight, 18 days after anthesis. both iaa levels and rates of ethylene evolution declined to their lowest levels (7 nanograms iaa per gram fresh weight and 0.01 nano ...198716665467
[active protein constituents from the kernel of prunus persica]. 19862952323
[peach (prunus persica) pollinosis in people who have not been engaged in growing peaches]. 19854038086
structural and biochemical aspects of peach fruit abscission (prunus persica l. batsch).the physiological drop of immature fruits was studied in relation to the activation of the abscission zone located between the fruit and the receptacle. light- and electron-microscopy observations demonstrated that this zone consisted of two types of parenchymatous cells: in the distal region, closer to the fruit, were groups of small thick-walled cells with few intercellular spaces; in the proximal region, closer to the pedicel, the stillgrouped cells were larger, polyphenolic-rich, and thick-w ...198524249493
properties of peach flower buds which facilitate supercooling.water in dormant peach (prunus persica [l.] batsch. var. ;harbrite') flower buds deep supercooled. both supercooling and the freezing of water within the bud axis and primordium as distinct components depended on the viability of the bud axis tissue. the viability of the primordium was not critical. supercooling was prevented by wounding buds with a dissecting needle, indicating that bud structural features were important. bud morphological features appeared to prevent the propagation of ice thr ...198216662701
leaf water potential, stomatal resistance, and photosynthetic response to water stress in peach seedlings.individual groups of peach (prunus persica [l.] batsch) seedlings stressed to -17, -26 and -36 bars recovered to control levels within 1, 3, and 4 days, respectively. stomatal resistance was significantly correlated with both leaf water potential and net photosynthesis. in seedlings stressed to -52 bars, leaf water potential and stomatal resistance recovered sooner than net photosynthesis, despite recovery of 0(2) evolution at a rate similar to leaf water potential. therefore, some nonstomatal f ...198216662343
[studies on the purgative drugs. iii. on the constituents of the flowers of prunus persica batsch (author's transl)]. 1977557087
some characteristics of ethylene production in peach (prunus persica l.) seeds.the seed of peach fruits develop the capacity to produce ethylene with a lag phase of about 1 h after excision. the site of ethylene synthesis is in the seed coat and rates as high as 6,000 μl kg(-1) h(-1) were recorded. ethylene production was reduced to less than 1% of the control by 10 μg/ml cycloheximide. although the tissue had only a small methionine pool, supplying the seed with exogenous methionine did not influence ethylene production at any stage of seed development. label from [u-(14) ...197624424902
the effect of (2-chloroethyl)phosphonic acid on the sink strength of developing peach (prunus persica l.) fruit.the sink strength of one of a pair of competing peach fruits was increased when the fruit was treated with (2-chloroethyl)phosphonic acid (ethephon) and gibberellic acid. ethephon increased the capacity of the treated fruit to attract (14)c-labelled assimilates at most stages of fruit development and was most effective when the level of endogenous ethylene produced by the fruit was lowest. the results are discussed in relation to the hypothesis that ethylene participates in the control of sink s ...197624424772
characteristics of photosynthesis in peach leaves.seasonal photosynthetic patterns of loring peach (prunus persica (l). batsch) leaves were monitored throughout the 1974 growing season. using leaves on detached branches, co2 exchange was measured under controlled light and temperature conditions. net photosynthesis rate varied from 9 to 12.4 mg co2 dm(-2) leaf area h(-1) and was correlated with fruit development, with the highest values occurring during the final stage of fruit maturation. the proximity of fruits to leaves also regulated photos ...197524430151
separation and characterization of endopolygalacturonase and exopolygalacturonase from peaches.two polygalacturonases (pg i and pg ii) have been separated from extracts of ripe peaches (prunus persica) by chromatography on sephadex g-100. pg i hydrolyzes polygalacturonic acid from the nonreducing ends of the molecules, releasing galacturonic acid as the product. it functions optimally at ph 5.5, requires ca(2+) for activity, and hydrolyzes low molecular weight substrates most rapidly. in contrast, pg ii cleaves the molecular chain of the substrate randomly with a ph optimum at about 4. th ...197316658542
diffusible and extractable growth regulators in normal and dwarf shoot apices of peach, prunus persica batsch. 197116657743
effect of phloroglucinol and resorcinol on the clingstone peach polyphenol oxidase-catalyzed oxidation of 4-methylcatechol.phloroglucinol and resorcinol are not substrates for clingstone peach (prunus persica) polyphenol oxidase, but they react with 4-methyl-o-quinone, produced either enzymatically or nonenzymatically, to give an intense red or red-brown color with a maximal absorption at about 470 nanometers. several colored products were isolated from an ethyl acetate extract of the reaction by two-dimensional thin layer chromatography. based on thin layer chromatographic and spectral studies of the enzymatic and ...197116657727
isolation and characterization of polyphenol oxidase isozymes of clingstone peach.the polyphenol oxidase system in clingstone peach (prunus persica) was investigated. polyacrylamide disc-gel electrophoresis indicated four bands with polyphenol oxidase activity in extracts from acetone powder of clingstone peach. these four isozymes were then isolated from a buffer extract of peach acetone powder by cold acetone precipitation, followed by diethylaminoethyl cellulose column chromatography. all isozymes had different heat stabilities. at 55 c, polyphenol oxidases a, b, and d had ...197116657726
[not available]. 194718918161
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