| comparative est transcript profiling of peach fruits under different post-harvest conditions reveals candidate genes associated with peach fruit quality. | cold storage is used to inhibit peach fruit ripening during shipment to distant markets. however, this cold storage can negatively affect the quality of the fruit when it is ripened, resulting in disorders such as wooliness, browning or leathering. in order to understand the individual and combined biological effects that factors such as cold storage and ripening have on the fruit and fruit quality, we have taken a comparative est transcript profiling approach to identify genes that are differen ... | 2009 | 19744325 |
| biochemical and proteomic analysis of 'dixiland' peach fruit (prunus persica) upon heat treatment. | shipping of peaches to distant markets and storage require low temperature; however, cold storage affects fruit quality causing physiological disorders collectively termed 'chilling injury' (ci). in order to ameliorate ci, different strategies have been applied before cold storage; among them heat treatment (ht) has been widely used. in this work, the effect of ht on peach fruit quality as well as on carbon metabolism was evaluated. when fruit were exposed to 39 degrees c for 3 d, ripening was d ... | 2009 | 19734260 |
| influence of postharvest treatments on quality, carotenoids, and abscisic acid content of stored "spring belle" peach (prunus persica) fruit. | the influence of four postharvest treatments, 1-methylcyclopropene (1-mcp), carbon dioxide (co2), and nitrogen (n2), followed by fruit storage at 10 degrees c or of hydrocooling (h2o) at 1 degrees c, followed by storage at 0 degrees c on fruit quality, carotenoids, and abscisic acid (aba) content as well as on ethylene and carbon dioxide production of "spring belle" peach fruits, has been examined. ethylene production was reduced by all the treatments and raised after transfer the fruits at 20 d ... | 2009 | 19722584 |
| selection of reliable reference genes for gene expression studies in peach using real-time pcr. | rt-qpcr is a preferred method for rapid and reliable quantification of gene expression studies. appropriate application of rt-qpcr in such studies requires the use of reference gene(s) as an internal control to normalize mrna levels between different samples for an exact comparison of gene expression level. however, recent studies have shown that no single reference gene is universal for all experiments. thus, the identification of high quality reference gene(s) is of paramount importance for th ... | 2009 | 19619301 |
| dormancy-associated mads genes from the evg locus of peach [prunus persica (l.) batsch] have distinct seasonal and photoperiodic expression patterns. | mapping and sequencing of the non-dormant evg mutant in peach [prunus persica (l.) batsch] identified six tandem-arrayed dam (dormancy-associated mads-box) genes as candidates for regulating growth cessation and terminal bud formation. to narrow the list of candidate genes, an attempt was made to associate bud phenology with the seasonal and environmental patterns of expression of the candidates in wild-type trees. the expression of the six peach dam genes at the evg locus of peach was character ... | 2009 | 19553369 |
| evaluation of the antioxidant capacity, phenolic compounds, and vitamin c content of different peach and nectarine [ prunus persica (l.) batsch] breeding progenies. | antioxidant capacity and contents of total phenolics, anthocyanins, flavonoids, and vitamin c were evaluated in 218 genotypes from 15 peach and nectarine breeding progenies. significant differences were found among progenies on the fruit antioxidant profile, corroborated by the high contribution showed by cross to the phenotypic variance of each phytochemical trait analyzed (16-45%). phytochemical profile varied depending on peach/nectarine and yellow/white flesh color qualitative traits. on the ... | 2009 | 19397288 |
| comparative expression and transcript initiation of three peach dehydrin genes. | dehydrin genes encode proteins with demonstrated cryoprotective and antifreeze activity, and they respond to a variety of abiotic stress conditions that have dehydration as a common component. two dehydrins from peach (prunus persica l. [batsch.]) have been previously characterized; here, we describe the characterization of a third dehydrin from peach bark, ppdhn3, isolated by its response to low temperature. the expression of all three dehydrin genes was profiled by semi-quantitative reverse tr ... | 2009 | 19360436 |
| the peach (prunus persica [l.] batsch) homeobox gene knope3, which encodes a class 2 knotted-like transcription factor, is regulated during leaf development and triggered by sugars. | class 1 knotted1-like transcription factors (knox) are known to regulate plant development, whereas information on class 2 knox has been limited. the peach knope3 gene was cloned, belonged to a family of few class 2 members and was located at 66 cm in the prunus spp. g1 linkage-group. the mrna localization was diversified in leaf, stem, flower and drupe, but recurred in all organ sieves, suggesting a role in sap nutrient transport. during leaf development, the mrna earliest localized to primordi ... | 2009 | 19333623 |
| differential expression of alpha-l-arabinofuranosidase and alpha-l-arabinofuranosidase/beta-d-xylosidase genes during peach growth and ripening. | arabinose is the major neutral sugar in peach (prunus persica (l.) batsch) cell walls and substantial changes in arabinose content take place not only during peach melting, when a rapid-softening-related depolymerizing activity may be expected, but also at the onset of peach ripening. a full-length cdna clone sequence referred to as pparf1 (genbank accession no. dq486870) was obtained and determined by bioinformatics' analysis to be a peach alpha-l-arabinofuranosidase homologue. the deduced ppar ... | 2009 | 19303789 |
| carbon autonomy of peach shoots determined by (13)c-photoassimilate transport. | we used (13)co(2) tracing and source-sink manipulation to determine if fruiting shoots of peach (prunus persica (l.) batsch) trees are autonomous or if they import carbon from neighboring shoots, and if the degree of shoot autonomy is influenced by the source-sink relationship of the shoot. in three experiments, leaf to fruit ratio (l:f) of selected fruiting shoots was moderately (2005 and 2006) or strongly (complete sink removal, 2006) altered to either enhance or inhibit movement of carbon fro ... | 2008 | 19193563 |
| jasmonate-induced ripening delay is associated with up-regulation of polyamine levels in peach fruit. | methyl jasmonate (mj, 0.20mm) and its synthetic analog n-propyl dihydrojasmonate (pdj, 0.22mm) were applied to peach fruit (prunus persica l. batsch) at a late developmental stage under field conditions (in planta). on the basis of a previously demonstrated jasmonate (ja)-induced ripening delay in peach, the effects of jas on the time course of the endogenous polyamine (pa) accumulation and expression of their biosynthetic genes arginine decarboxylase (adc), ornithine decarboxylase (odc), spermi ... | 2009 | 19185952 |
| influence of prunus spp., peach cultivars, and bark damage on oviposition choices by the lesser peachtree borer (lepidoptera: sesiidae). | an examination of oviposition choices by the lesser peachtree borer, synanthedon pictipes (grote and robinson) (lepidoptera: sesiidae), showed that wounded peach, prunus persica (l.) batsch, bark was attractive to females for oviposition. females responded to bark that was injured mechanically (e.g., hammer blows, knife cuts, pruning wounds), infested by lesser peachtree borer larvae or injured by disease. in fact, there was no difference in female oviposition response to knife cut wounds and kn ... | 2008 | 19161694 |
| [rp-hplc determination of flavonoids in several flowers]. | to develvp a rp-hplc method for the determination of flavonoids in fifteen kinds of flowers such as iris lacteal pall, prunus persica and rosa chinensis. | 2008 | 19160795 |
| peach ( prunus persica l. batsch) allergen-encoding genes are developmentally regulated and affected by fruit load and light radiation. | the fruits of rosaceae species may frequently induce allergic reactions in both adults and children, especially in the mediterranean area. in peach, true allergens and cross-reactive proteins may cause hypersensitive reactions involving a wide diversity of symptoms. three known classes of allergenic proteins, namely, pru p 1, pru p 3, and pru p 4, have been reported to be mostly involved, but an exhaustive survey of the proteins determining the overall allergenic potential, their biological func ... | 2009 | 19090764 |
| molecular cloning and expression analysis of an arginine decarboxylase gene from peach (prunus persica). | arginine decarboxylase (adc), one of the enzymes responsible for putrescine (put) biosynthesis, has been shown to be implicated in stress response. in the current paper attempts were made to clone and characterize a gene encoding adc from peach (prunus persica (l.) batsch, 'akatsuki'). rapid amplification of cdna ends (race) gave rise to a full-length adc cdna (ppadc) with a complete open reading frame of 2178 bp, encoding a 725 amino acid polypeptide. homology search and sequence multi-alignmen ... | 2009 | 18996450 |
| temperature-dependent bionomics and modeling of anarsia lineatella (lepidoptera: gelechiidae) in the laboratory. | laboratory studies were conducted to assess the effect of constant temperatures (15, 20, 25, 30, and 35 degrees c) on life history traits of peach twig borer, anarsia lineatella zeller (lepidoptera: gelechiidae). developmental rate, survival, longevity, and fecundity were studied in environmental chambers from individuals reared on peach, prunus persica l., twigs until adulthood. temperature-dependent relationships of various developmental events were characterized, and applied models were evalu ... | 2008 | 18950037 |
| [effects of alternative partial rootzone irrigation on peach growth, productivity, and water use efficiency]. | taking 12-year-old peach (prunus persica l. batsch cv. ruiguang 5) as test material, this paper studied the effects of alternative partial rootzone irrigation (apri) on its growth, productivity, and water use efficiency under semi-arid climate condition. the results showed that in apri treatments, the soil water content in the wet side of peach tree decreased with increasing soil depth, while that in the dry side was in adverse, with the maximal difference appeared in 0-25 cm soil layer. in the ... | 2008 | 18839930 |
| simple sequence repeat analysis of genetic diversity in primary core collection of peach (prunus persica). | in this study, the genetic diversity of 51 cultivars in the primary core collection of peach (prunus persica (l.) batsch) was evaluated by using simple sequence repeats (ssrs). the phylogenetic relationships and the evolutionary history among different cultivars were determined on the basis of ssr data. twenty-two polymorphic ssr primer pairs were selected, and a total of 111 alleles were identified in the 51 cultivars, with an average of 5 alleles per locus. according to traditional chinese cla ... | 2008 | 18666957 |
| molecular cloning, identification, and chromosomal localization of two mads box genes in peach (prunus persica). | mads box proteins play an important role in floral development. to find genes involved in the floral transition of prunus species, cdnas for two mads box genes, ppmads1 and ppmads10, were cloned using degenerate primers and 5'- and 3'-race based on the sequence database of p. persica and p. dulcis. the full length of ppmads1 cdna is 1,071 bp containing an open reading frame (orf) of 717 bp and coding for a polypeptide of 238 amino acid residues. the full length of ppmads10 cdna is 937 bp contain ... | 2008 | 18571125 |
| transcriptome profiling of ripening nectarine (prunus persica l. batsch) fruit treated with 1-mcp. | a large-scale transcriptome analysis has been conducted using micropeach1.0 microarray on nectarine (prunus persica l. batsch) fruit treated with 1-methylcyclopropene (1-mcp). 1-mcp maintained flesh firmness but did not block ethylene biosynthesis. compared with samples at harvest, only nine genes appeared to be differentially expressed when fruit were sampled immediately after treatment, while a total of 90 targets were up- or down-regulated in untreated fruit. the effect of 1-mcp was confirmed ... | 2008 | 18515268 |
| changes in aquaporin gene expression and magnetic resonance imaging of water status in peach tree flower buds during dormancy. | the movement of cellular water accompanies changes in growth within dormant buds. to further understand this process, accumulation of tonoplast deltatip1 and plasma membrane pip2 aquaporin transcripts was measured by quantitative reverse transcriptase-polymerase chain reaction and the water dynamics in dormant peach (prunus persica l.) flower buds was studied by magnetic resonance imaging. proton density (pd), spin-spin relaxation time (t(2)) and apparent diffusion coefficient (adc) were used to ... | 2008 | 18513373 |
| identification of woolliness response genes in peach fruit after post-harvest treatments. | woolliness is a physiological disorder of peaches and nectarines that becomes apparent when fruit are ripened after prolonged periods of cold storage. this disorder is of commercial importance since shipping of peaches to distant markets and storage before selling require low temperature. however, knowledge about the molecular basis of peach woolliness is still incomplete. to address this issue, a nylon macroarray containing 847 non-redundant expressed sequence tags (ests) from a ripe peach frui ... | 2008 | 18453640 |
| jasmonate-induced transcriptional changes suggest a negative interference with the ripening syndrome in peach fruit. | peach (prunus persica l. batsch) was chosen as a model to shed light on the physiological role of jasmonates (jas) during fruit ripening. to this aim, the effects of methyl jasmonate (mj, 0.40 mm) and propyl dihydrojasmonate (pdj, 0.22 mm), applied in planta at different fruit developmental stages, on the time-course of ethylene production and fruit quality traits were evaluated. mj-induced changes in fruit transcriptome at harvest and the expression profiling of relevant ja-responsive genes wer ... | 2008 | 18252703 |
| automatic discrimination of fine roots in minirhizotron images. | minirhizotrons provide detailed information on the production, life history and mortality of fine roots. however, manual processing of minirhizotron images is time-consuming, limiting the number and size of experiments that can reasonably be analysed. previously, an algorithm was developed to automatically detect and measure individual roots in minirhizotron images. here, species-specific root classifiers were developed to discriminate detected roots from bright background artifacts. classifiers ... | 2008 | 18042202 |
| the population level of eotetranychus lewisi and the concentration of carbohydrates in peach trees. | lewis spider mite eotetranychus lewisi (mcgregor) is the most important pest in peach orchards, prunus persica (l.) (batsch), in north-central mexico. in autumn 2003 and spring 2004, two glasshouse experiments were carried out to assess the influence of that mite on the concentration of total soluble sugars and starch in leaves, bark and roots of 'diamante mejorado' peach trees. apical leaves of peach trees were inoculated with three mite densities per leaf: (a) 10-20, (b) 21-40, (c) 41-80; a mi ... | 2007 | 18040869 |
| vascular flows and transpiration affect peach (prunus persica batsch.) fruit daily growth. | the relative contributions of xylem, phloem, and transpiration to fruit growth and the daily patterns of their flows have been determined in peach, during the two stages of rapid diameter increase, by precise and continuous monitoring of fruit diameter variations. xylem, phloem, and transpiration contributions to growth were quantified by comparing the diurnal patterns of diameter change of fruits, which were then girdled and subsequently detached. xylem supports peach growth by 70%, and phloem ... | 2007 | 18037679 |
| partial purification of latent polyphenol oxidase from peach (prunus persica l. cv. catherina). molecular properties and kinetic characterization of soluble and membrane-bound forms. | this paper analyzes the kinetic and structural characteristics of polyphenol oxidase (ppo) from peach cv. catherina. the ppo was obtained in a latent state in both the soluble and membrane-bound forms, and both forms were activated by acid shock and the detergent sds. plant defense is the main function assigned to ppo, which would be activated by the acid environment resulting from tissue damage. on the other hand, it has been suggested that, physiologically, the role played by sds may be fulfil ... | 2007 | 17994689 |
| the effects of foot and facial massage on sleep induction, blood pressure, pulse and respiratory rate: crossover pilot study. | the study aimed to compare the effects of facial massage with that of foot massage on sleep induction and vital signs of healthy adults and to test a methodology that could be used by a lone researcher in such a study. a randomised within-group crossover pilot study of six healthy female volunteers was conducted. the interventions were a 20min foot and a 20min facial massage using peach-kernel base oil prunus persica. a drop in systolic blood pressure of 8.5mmhg was recorded immediately after fa ... | 2007 | 17950182 |
| evaluation of sap flow and trunk diameter sensors for irrigation scheduling in early maturing peach trees. | five-year-old early maturing peach trees (prunus persica (l.) batsch cv. flordastar grafted on gf-677 peach rootstock) were subjected to three irrigation treatments from march 18 to november 10, 2006. control plants (t0 treatment) which received irrigation in excess of their crop water requirements (1089.7 mm) were compared with plants watered according to sap flow (sf; t1 treatment) or maximum daily trunk shrinkage (mds; t2 treatment) measurements, so as to maintain sf and mds signal intensitie ... | 2007 | 17938106 |
| effects of post-harvest light conditions on quality and aromatic volatile formation in 'hakuho' peach (prunus persica batsch) fruits. | the effect of light condition during post-harvest storage on fruit quality of 'hakuho' peach (prunus persica batsch) was examined. fruits were harvested at the immature stage (7 d before the tree-ripening stage) and firm-ripe (3 d before the tree-ripening) stage and stored at 25 degrees c under light (ca 80 micromol m(-2) s(-1) at the fruit top by a fluorescent lamp) and in darkness. the light and dark conditions did not significantly influence the ethylene production rate except for the fully r ... | 2007 | 17556807 |
| [effects of exogenous betaine on physiological responses of peach tree under water stress]. | with four-year-old potted prunus persica l. cv. qingfeng as test material, this paper studied the change pattern of its leaf betaine content under water stress, and its physiological responses under effects of foliage-spraying exogenous betaine. the results showed that under normal water supply, the betaine content in qingfeng' s leaf was 75.9-80.5 microg x g(-1) fm, which was increased with increasing water stress, and up to 278.9 microg x g(-1) fm on the 16th day after cutting off the water su ... | 2007 | 17552190 |
| photosynthesis in response to sink-source activity and in relation to end products and activities of metabolic enzymes in peach trees. | fruits were removed (-fruit) or retained (+fruit) on limbs of 'yanfengyihao' peach (prunus persica (l.) batch) trees. in addition to the -fruit and +fruit treatments, leaves on 1-year-old fruit-bearing shoots of 'bayuecui' peach trees were covered with bags for varying periods of time. net photosynthetic rate (p(n)), stomatal conductance and transpiration rate around midday significantly decreased in the -fruit treatment and significantly increased in bagged leaves after removal of the bags comp ... | 2007 | 17545130 |
| molecular cloning and expression analysis of cdnas encoding a putative nrt2 nitrate transporter from peach. | two cdna clones encoding a putative high-affinity nitrate transporter (nrt2) were isolated from a cdna library of peach (prunus persica (l.) batsch) roots. the nucleotide sequences of the clones, named ppnrt2.1 and ppnrt2.2, showed 99% identity in their coding regions, but their 3'-untranslated sequences showed only 40% identity. the deduced amino acid sequences of the clones showed 44-90% homology to nrt2 proteins from other plants. both clones contained open reading frames encoding polypeptide ... | 2007 | 17241992 |
| growth patterns and morphology of fine roots of size-controlling and invigorating peach rootstocks. | we compared growth patterns and morphology of fine roots of size-controlling and invigorating peach (prunus persica (l.) batsch) rootstocks. peach trees were grafted on five rootstocks: a vigorous control (nemaguard), three intermediate vigor rootstocks (k119-50, p30-135 and hiawatha), and a semi-dwarfing rootstock (k146-43). minirhizotron tubes were installed at the base of trees on each rootstock and root images captured with a minirhizotron digital camera system. number, visible length, and d ... | 2007 | 17241965 |
| current-year and subsequent-year effects of crop-load manipulation and epicormic-shoot removal on distribution of long, short and epicormic shoot growth in prunus persica. | the distribution of canopy growth among different shoot types such as epicormic, long and short shoots is not well understood in the peach tree. in this experiment, the effects of crop load and early epicormic sprout removal on current and subsequent-year distribution of vegetative growth among epicormic, long and short shoots was investigated in prunus persica. | 2007 | 17218345 |
| daily sap flow and maximum daily trunk shrinkage measurements for diagnosing water stress in early maturing peach trees during the post-harvest period. | we compared the sensitivity of two continuously recorded plant-based water stress indicators (sap flow, sf, and maximum daily trunk shrinkage, mds) to detect changes in the water status of 4-year-old early maturing peach trees (prunus persica (l.) batsch cv. flordastar grafted on gf-677 peach rootstock) during a cycle of deficit irrigation and recovery. the feasibility of obtaining sf and mds reference equations for use in irrigation scheduling during the post-harvest period was also studied in ... | 2007 | 17169909 |
| changes in cell wall polysaccharide of harvested peach fruit during storage. | changes in cell wall structure, decrease in tissue firmness and ethylene production in honey peach [prunus persica (l.) batsch, cv. 'yuhuasanhao'] were investigated after various storage at 5 degrees c and at 20 degrees c. the results showed that during storage, the peak of ethylene production lagged significantly behind the rapid softening of peach fruit, which means that it is not ethylene causes the softening of peach fruit. the structural and compositional changes of cell wall of fruits stor ... | 2006 | 17167202 |
| effects of nitric oxide on fatty acid composition in peach fruits during storage. | peach (prunus persica (l.) batsch., cv. feicheng) fruits were fumigated with 0, 5, 10, and 15 microl/l nitric oxide (no), and the effects of no on rot index, ion leakage, and the lipid compositions in peach fruits were studied. the results showed that the treatment with 10 microl/l no significantly increased the contents of the palmitoleic (16:1), oleic (18:1), and linolenic (18:3) acid in phospholipid (pl), while it decreased the contents of linoleic (18:2) acid in pl. the content of monoacylgl ... | 2006 | 17147431 |
| transcription of ethylene perception and biosynthesis genes is altered by putrescine, spermidine and aminoethoxyvinylglycine (avg) during ripening in peach fruit (prunus persica). | the time course of ethylene biosynthesis and perception was investigated in ripening peach fruit (prunus persica) following treatments with the polyamines putrescine (pu) and spermidine (sd), and with aminoethoxyvinylglycine (avg). fruit treatments were performed in planta. ethylene production was measured by gas chromatography, and polyamine content by high-performance liquid chromatography; expression analyses were performed by northern blot or real-time polymerase chain reaction. differential ... | 2006 | 16995911 |
| changes in free polyamine titers and expression of polyamine biosynthetic genes during growth of peach in vitro callus. | in the present paper, correlation between free polyamines and growth of peach (prunus persica cv. yuzora) in vitro callus was investigated. growth of the callus was divided into three phases based on measurement of fresh weight. free polyamines, putrescine (put), spermidine (spd), and spermine (spm), could be detected during peach callus growth. changes in free put titers followed the callus growth rate, as shown by low and stable levels in the first stage, quick increase at the beginning of the ... | 2007 | 16912865 |
| activity and subcellular localization of glucose-6-phosphate dehydrogenase in peach fruits. | the subcellular distribution and activity of glucose-6-phosphate dehydrogenase (g6pdh, ec 1.1.1.49) were studied in developing peach (prunus persica l. batsch cv. zaoyu) fruit. fruit tissues were separated by differential centrifugation at 15,000g into plastidic and cytosolic fractions. there was no serious loss of enzyme activity (or activation) during the preparation of fractions. g6pdh activity was found in both the plastidic and cytosolic compartments. moreover, dtt had no effect on the plas ... | 2007 | 16884825 |
| changes in endopolygalacturonase levels and characterization of a putative endo-pg gene during fruit softening in peach genotypes with nonmelting and melting flesh fruit phenotypes. | the changes in endopolygalacturonase (endo-pg) levels and endo-pg expression in nonmelting flesh (nmf) and melting flesh (mf) peach fruits (prunus persica) during softening were studied. the endo-pg gene was analysed to identify polymorphisms exploitable for early marker-assisted selection (mas) of flesh texture. the role of endo-pg in softening was assessed by western and northern blotting and by biochemical analyses. polymorphisms in the endo-pg gene were revealed by reverse transcription-poly ... | 2006 | 16866939 |
| food protective and color alteration effects of acaricidal aldehydes on tyrophagus putrescentiae (schrank). | the activities of benzaldehyde isolated from prunus persica seeds and of commercially available aldehydes against tyrophagus putrescentiae (a stored-food mite) adults were examined and compared with those of the synthetic acaricides benzyl benzoate and n,n-diethyl-m-toluamide. on the basis of the 50% lethal dose (ld50), the compound most toxic to t. putrescentiae adults was salicylaldehyde (ld50 of 1.02 microg/cm2) followed by cinnamaldehyde (1.66 microg/cm2), benzaldehyde (4.23 microg/cm2), pht ... | 2006 | 16865912 |
| hydraulic conductance characteristics of peach (prunus persica) trees on different rootstocks are related to biomass production and distribution. | we investigated hydraulic conductance characteristics and associated dry matter production and distribution of peach trees grafted on different rootstocks growing in the field. a single scion genotype was grown on a low ('k146-43'), an intermediate ('hiawatha') and a high ('nemaguard') vigor rootstock. 'k146-43' and 'hiawatha' rootstocks had 27 and 52% lower mean leaf-specific hydraulic conductances, respectively, than the more vigorous 'nemaguard' rootstock. tree growth rates and patterns of bi ... | 2006 | 16815836 |
| relationship of water status to vegetative growth and leaf gas exchange of peach (prunus persica) trees on different rootstocks. | we investigated relationships between tree water status, vegetative growth and leaf gas exchange of peach trees growing on different rootstocks under field conditions. tree water status was manipulated by partially covering (0, approximately 30 and approximately 60%) the tree canopies on individual days and then evaluating the effects of tree water status on vegetative growth and leaf gas exchange. early morning stem water potentials were approximately -0.4 mpa for trees in all treatments, but m ... | 2006 | 16815835 |
| effects of fruit pre-harvest bagging on fruit quality of peach (prunus persica batsch cv. hujingmilu). | experiments were conducted to test the effects of pre-harvest bagging on fruit ripening and quality of peach (prunus persica batsch cv. hujingmilu). young fruits, at 50 days after full bloom (dafb), were covered with bags made of single-, double-, and triple-layers of orange paper bag with 27.0%, 13.9% and 8.2% sunlight transmission, respectively. ethylene production and respiration rate were measured, and fruit quality was analyzed at 111, 114, 117, 120 (firm-ripe stage) and 124 dafb (full-ripe ... | 2006 | 16775394 |
| the effect of root pressurization on water relations, shoot growth, and leaf gas exchange of peach (prunus persica) trees on rootstocks with differing growth potential and hydraulic conductance. | it is well known that rootstocks can have an effect on the vegetative growth and development of the tree; however, there has been no clear explanation about the physiological mechanism involved in this phenomenon. evidence indicates that the rootstock effects on tree vegetative growth may be related to hydraulic limitations of the rootstock. the objective of these experiments was to investigate the shoot growth, water potential, and gas exchange of peach trees on different rootstocks in response ... | 2006 | 16690626 |
| [the relation between en-dormancy and changes in two main electron transport pathways of nectarine (prunus persica var. nectariana) buds]. | respiration rate of buds of prunus persica var. nectariana maxim. cv. shuguang and mayfire was studied during dormancy. the results showed that the bud respiration rate changed with the stage of dormancy for both cultivars. respiration rate of buds of the controls decreased steadily before dormancy, while increased slowly from november 5th for shuguang and october 29th for mayfire, and then decreased sharply on december 17th at which dormancy was terminated. cyanide (kcn) only partially inhibite ... | 2006 | 16622313 |
| the involvement of 1-aminocyclopropane-1-carboxylic acid synthase isogene, pp-acs1, in peach fruit softening. | ethylene promotes fruit ripening, including softening. the fruit of melting-flesh peach (prunus persica (l). batsch) cultivar 'akatsuki' produces increasing levels of ethylene, and the flesh firmness softens rapidly during the ripening stage. on the other hand, the fruit of stony hard peach cultivars 'yumyeong', 'odoroki', and 'manami' does not soften and produces little ethylene during fruit ripening and storage. to clarify the mechanism of suppression of ethylene production in stony hard peach ... | 2006 | 16531466 |
| role of polyamines in peach fruit development and storage. | to investigate the role of polyamines in pre- and post-harvest fruit development of 'akatsuki' peach (prunus persica (l.) batsch.) we measured polyamine concentrations, activities of polyamine biosynthetic enzymes and expression of genes encoding these enzymes. concentrations of the free polyamines, putrescine (put), spermidine (spd) and spermine (spm) in pre-harvest fruit peaked 16 days after full bloom (daf) and then progressively decreased until harvest with the exception of put, which showed ... | 2006 | 16510395 |
| differential regulation of two dehydrin genes from peach (prunus persica) by photoperiod, low temperature and water deficit. | dehydrins are one of several proteins that have been specifically associated with qualitative and quantitative changes in cold hardiness. recent evidence indicates that the regulation of dehydrin genes by low nonfreezing temperature (lt) and short photoperiod (sd) can be complex and deserves more detailed analysis to better understand the role of specific dehydrin genes and proteins in the response of woody plants to environmental stress. we have identified a new peach (prunus persica (l.) batsc ... | 2006 | 16452071 |
| preformation in vegetative buds of prunus persica: factors influencing number of leaf primordia in overwintering buds. | we investigated the influence of bud position, cultivar, tree age, tree carbohydrate status, sampling date, drought and light exposure on the number of leaf primordia formed in dormant vegetative peach buds (prunus persica (l.) batsch) relative to the number of primordia formed after bud break (neoformed). during winter dormancy, vegetative peach buds from california and italy were dissected and the number of leaf primordia recorded. between leaf drop and bud break, the number of leaf primordia ... | 2006 | 16414932 |
| ppeg4 is a peach endo-beta-1,4-glucanase gene whose expression in climacteric peaches does not follow a climacteric pattern. | in peach (prunus persica l. batsch.) the degradation of the pectic compounds of the cell wall is considered to be the principal component responsible for fruit softening. many genes encoding enzymes acting on the different polymers of the pectic matrix have been shown to be highly expressed during the late phases of softening, with polygalacturonase being the most important. nevertheless, it is known that softening starts well before the ethylene climacteric rise which occurs concomitant with th ... | 2006 | 16410260 |
| estree db: a tool for peach functional genomics. | the estree db http://www.itb.cnr.it/estree/ represents a collection of prunus persica expressed sequenced tags (ests) and is intended as a resource for peach functional genomics. a total of 6,155 successful est sequences were obtained from four in-house prepared cdna libraries from prunus persica mesocarps at different developmental stages. another 12,475 peach est sequences were downloaded from public databases and added to the estree db. an automated pipeline was prepared to process est sequen ... | 2005 | 16351742 |
| identification and characterization of armillaria tabescens from the southeastern united states. | in the southeastern usa, armillaria root rot disease on peach (prunus persica) is caused by armillaria tabescens, and to a lesser degree by a. mellea. recent attempts to genetically characterize a. tabescens isolates using rdna indicated the existence of heterozygosity in diploid isolates. in order to clarify this heterozygosity, dna from stipe and single spore cultures of a. tabescens isolate sc.mf-1.01 was characterized using rflp and sequence analysis. direct sequencing of rdna amplicons from ... | 2005 | 16279414 |
| cloning and characterization of a non-tir-nbs-lrr type disease resistance gene analogue from peach. | cloning of plant disease resistant genes is greatly helpful for disease resistant breeding in plants and the insight of resistance mechanism. however, there are less relevant researches in peach [prunus persica (l.) batch]. in this study, four nbs-lrr type resistance gene analogs (rgas) were cloned from genomic dna of peach. the pnbs2 fragment was also amplified from peach cdna and the full-length cdna of pnbs2 (prpm1, genbank accession no. ay599223) has been cloned. sequence analysis indicated ... | 2005 | 16147861 |
| ethy. a theory of fruit climacteric ethylene emission. | a theory of fruit climacteric ethylene emission was developed and used as the basis of a simulation model called ethy. according to the theory, the biosynthetic pathway of ethylene is supplied by atp and is regulated by 1-aminocyclopropane-1-carboxylic acid (acc) synthase and acc oxidase. the conjugation of acc with malonate to form macc was taken into account as a way to decrease the availability of acc. because of the seasonal increase of fruit volume, the dilution of biochemical compounds use ... | 2005 | 16143642 |
| microsatellite and aflp markers in the prunus persica [l. (batsch)]xp. ferganensis bc(1)linkage map: saturation and coverage improvement. | a set of 146 single sequence repeats (ssrs) and 14 amplified fragment length polymorphism (aflp) primer combinations were used to enrich a previously developed linkage map obtained from a (prunus persicaxp. ferganensis)xp. persica bc(1) progeny. forty-one ssr primer pairs gave polymorphic patterns detecting 42 loci. the restriction/selective primer aflp combinations produced a total of 79 segregating fragments. the resulting map is composed of 216 loci covering 665 cm with an average distance of ... | 2005 | 16088395 |
| a virtual peach fruit model simulating changes in fruit quality during the final stage of fruit growth. | a virtual fruit model simulating seasonal changes in several peach (prunus persica (l.) batsch) fruit quality traits during the final growth stage is presented. the quality traits considered are fruit size, the proportion of total fruit mass consisting of fruit flesh, dry matter content of the flesh and the concentrations of sucrose, glucose, fructose and sorbitol in the flesh, which are used to calculate a sweetness index. the virtual peach fruit model was developed by adapting and integrating ... | 2005 | 16076779 |
| a rapid and efficient method for purifying high quality total rna from peaches (prunus persica) for functional genomics analyses. | prunus persica has been proposed as a genomic model for deciduous trees and the rosaceae family. optimized protocols for rna isolation are necessary to further advance studies in this model species such that functional genomics analyses may be performed. here we present an optimized protocol to rapidly and efficiently purify high quality total rna from peach fruits (prunus persica). isolating high-quality rna from fruit tissue is often difficult due to large quantities of polysaccharides and pol ... | 2005 | 15977413 |
| candidate gene database and transcript map for peach, a model species for fruit trees. | peach (prunus persica) is a model species for the rosaceae, which includes a number of economically important fruit tree species. to develop an extensive prunus expressed sequence tag (est) database for identifying and cloning the genes important to fruit and tree development, we generated 9,984 high-quality ests from a peach cdna library of developing fruit mesocarp. after assembly and annotation, a putative peach unigene set consisting of 3,842 ests was defined. gene ontology (go) classificati ... | 2005 | 15846479 |
| changes in aroma volatile compounds and ethylene production during "hujingmilu" peach (prunus persica l.) fruit development. | changes in ethylene production rate, respiration rate, and aroma volatile composition was determined from the immature to mature stage in "hujingmilu" peach (prunus presica) fruit. flesh firmness, fruit size, ground color of skin, and total soluble solids (tss), titratable acidity (ta) in juice were analyzed, and acc, acc synthase and acc oxidase activities were measured. with the increase of fruit maturity, fruit size, l* value, and tss and major reducing sugar contents increased, whereas fruit ... | 2005 | 15692177 |
| characterization of an s-locus receptor protein kinase-like gene from peach. | a receptor-like protein kinase gene (ppsrkl1) was isolated from a peach (prunus persica (l.) batsch.) bark cdna library prepared with rnas isolated from bark collected in december (cold acclimated). sequence analysis indicated that this gene is related to the s-locus family of receptor protein kinases (srks) and that it shares greatest homology with zmpk1 from maize and at4g32300 from arabidopsis, both of which are intron-less genes. in bark tissues, ppsrkl1 is induced by water deficit treatment ... | 2005 | 15687089 |
| modelling the effect of fruit growth on surface conductance to water vapour diffusion. | a model of fruit surface conductance to water vapour diffusion driven by fruit growth is proposed. it computes the total fruit conductance by integrating each of its components: stomata, cuticle and cracks. | 2005 | 15655107 |
| presence of allergenic proteins in different peach (prunus persica) cultivars and dependence of their content on fruit ripening. | it has been reported that various cultivars of fruits and vegetables may present a different pattern for the contained allergens. here, we report on the different content in allergenic proteins for different peach (prunus persica) cultivars, sampled during two consecutive harvest seasons. fruits from six cultivars of peaches were harvested fully ripe, and the proteins extracted from whole or chemically peeled fruits were analyzed by sds-page and immunoblotting. all the protein extracts from whol ... | 2004 | 15612787 |
| cell wall metabolism during the development of chilling injury in cold-stored peach fruit: association of mealiness with arrested disassembly of cell wall pectins. | partially tree-ripened ripe fruit of peach (prunus persica l.) were stored for 1-4 weeks at 5 degrees c and then ripened at 20 degrees c for 3 d to induce chilling injury. with increasing cold storage the incidence and severity of mealiness symptoms increased progressively, manifested as reduced quantities of free juice and internal flesh browning. relative to juicy fruit, tissue of mealy fruit showed altered intercellular adhesion when examined by microscopy and, upon crushing, a higher proport ... | 2004 | 15310820 |
| cell wall metabolism during maturation, ripening and senescence of peach fruit. | cell wall changes were examined in fruit of a melting flesh peach (prunus persica l.) allowed to ripen on the tree. three phases to softening were noted, the first of which began prior to the completion of flesh colour change and an increase in ethylene evolution. softening in young mature fruit, prior to ripening, was associated with a depolymerization of matrix glycans both loosely and tightly attached to cellulose and a loss of gal from all cell wall fractions. after the initiation of ripenin ... | 2004 | 15286150 |
| developmental regulation of peach acc oxidase promoter--gus fusions in transgenic tomato fruits. | a genomic dna sequence (ppaco1) encoding 1-aminocyclopropane-1-carboxylic acid oxidase (aco) from peach (prunus persica l. batsch cv. loring) was isolated. it has four exons interrupted by three introns and 2.9 kb of flanking region 5' of the translational start codon. previous work with the cdna demonstrated that accumulation of the peach aco message correlated with increasing amounts of ethylene synthesized by the fruit as they ripened. to identify regulatory elements in the peach acc oxidase ... | 2004 | 15208348 |
| trophic control of bud break in peach (prunus persica) trees: a possible role of hexoses. | vegetative buds of peach (prunus persica l. batsch.) trees act as strong sinks and their bud break capacity can be profoundly affected by carbohydrate availability during the rest period (november-february). analysis of xylem sap revealed seasonal changes in concentrations of sorbitol and hexoses (glucose and fructose). sorbitol concentrations decreased and hexose concentrations increased with increasing bud break capacity. sucrose concentration in xylem sap increased significantly but remained ... | 2004 | 14996662 |
| sorbitol uptake is regulated by glucose through the hexokinase pathway in vegetative peach-tree buds. | in peach trees (prunus persica l. batsch cv. redhaven), sorbitol is a primary photosynthetic product and may play an important role in the budbreak process. surprisingly, before budbreak (from january to early march), the concentration of sorbitol in the xylem sap decreases, while that of hexoses (glucose and fructose) increases. the aim of this work was to study the control of sorbitol uptake into vegetative buds by hexoses. sorbitol uptake was selectively inhibited by hexoses at low and physio ... | 2004 | 14990620 |
| respiratory allergy to peach leaves and lipid-transfer proteins. | several lipid-transfer proteins (ltps) have been identified as important food allergens, especially in fruits of the rosaceae family. the major peach (prunus persica) allergen has been identified, sequenced and designated pru p 3. | 2004 | 14987310 |
| distribution and partial characterization of seasonally expressed proteins in different aged shoots and roots of 'loring' peach (prunus persica). | during autumnal leaf senescence, leaf nitrogen in deciduous trees is translocated to storage sites, especially bark and xylem tissues. proteins that accumulate in large amounts in bark and xylem in winter and are absent from these organs in summer are called storage proteins, and are believed to be vehicles for storing nitrogen reserves. these reserves are important for spring growth and help trees tolerate or recover from both abiotic and biotic stresses. based on seasonal patterns of accumulat ... | 2004 | 14704143 |
| does variability in shoot carbon assimilation within the tree crown explain variability in peach fruit growth? | a three-dimensional model of radiative transfer and leaf gas exchange was used to quantify daily carbon (c) assimilation of all fruit-bearing shoots (fbs) in an early maturing 6-year-old peach tree (prunus persica l. batsch) with a heavy crop load. for a sample of fbs (n=36), growth of fruit and leafy shoots was measured every 1-2 weeks from 24 days after bloom (dab) until harvest, between 93-101 dab. the objective was to relate shoot c assimilation with harvested fruit mass for each shoot to te ... | 2004 | 14704140 |
| peach (prunus persica l.) fruit growth and ripening: transcript levels and activity of polyamine biosynthetic enzymes in the mesocarp. | transcript levels and activities of the polyamine biosynthetic enzymes arginine decarboxylase (adc, ec 4.1.1.19), ornithine decarboxylase (odc, ec 4.1.1.17) and s-adenosylmethionine decarboxylase (samdc, ec 4.1.1.21), as well as free polyamine titres, were analysed throughout the four growth stages s1-s4 leading up to ripening in the mesocarp from peach fruit (prunus persica l. batsch cv. redhaven) grown under field conditions. samdc mrna, which was northern analysed by using a pcr-generated hom ... | 2003 | 14593813 |
| isolation and characterization of a maintenance dna-methyltransferase gene from peach (prunus persica [l.] batsch): transcript localization in vegetative and reproductive meristems of triple buds. | a cdna coding for a dna (cytosine-5)-methyltransferase (metase) was isolated from peach (prunus persica [l.] batsch) and the corresponding gene designated as ppmeti. the latter encoded a predicted polypeptide of 1564 amino acid residues and harboured all the functional domains conserved in the maintenance metases group type i. ppmeti was a single copy in the cultivar chiripa which was used as a model in the present study. expression analyses revealed that ppmeti transcripts were more abundant in ... | 2003 | 14563834 |
| investigation of bound aroma constituents of yellow-fleshed nectarines (prunus persica l. cv. springbright). changes in bound aroma profile during maturation. | glycosidically bound volatile constituents of yellow-fleshed clingstone nectarines (cv. springbright) were identified and quantified at three stages of maturity. glycoconjugates were isolated by lc on a c(18) reversed phase column with methanol elution followed by hydrolysis with a commercial pectinase enzyme. forty-five bound aglycons were identified for the first time in yellow-fleshed nectarine. thirty were terpene derivatives, and the most abundant ones were (e)- and (z)-furan linalool oxide ... | 2003 | 14518956 |
| effects of fe deficiency chlorosis on yield and fruit quality in peach (prunus persica l. batsch). | the effects of iron (fe) deficiency on fruit yield and quality were measured in two peach cultivars, carson (yellow-skin fruit) and babygold (red-skin fruit). in both cultivars, fe deficiency caused major decreases in fruit fresh weight per tree and number of fruits per tree. fruits from fe-deficient peach trees had a smaller size, resulting in a large decrease in the percentage of commercially acceptable fruits, whereas fruit firmness was unaffected. in cv. babygold, fe deficiency greatly decre ... | 2003 | 12952427 |
| a cell wall-oriented genomic approach reveals a new and unexpected complexity of the softening in peaches. | during ripening, fleshy fruits undergo textural changes that lead to loss of tissue firmness and consequent softening. it is a common idea that this process is the consequence of cell wall dismantling carried out by different and orderly expressed enzymes. for this purpose, by using a single enzyme family approach many enzymes and related genes have been characterized in different fruits. in this work, the softening of the climacteric peach fruits (prunus persica (l.) batsch.) has been studied b ... | 2003 | 12815031 |
| daily shoot extension growth of peach trees growing on rootstocks that reduce scion growth is related to daily dynamics of stem water potential. | we studied relationships between diurnal patterns of stem water potential (psistem) and stem extension growth of the same scion cultivar growing on three rootstocks with differing size-controlling potentials. the peach trees (prunus persica (l.) batsch) used in this field experiment consisted of an early-maturing freestone cultivar, 'flavorcrest,' grafted onto three different rootstocks: nemaguard (a vigorous seed-propagated control, p. persica x p. davidiana hybrid), hiawatha (an intermediate v ... | 2003 | 12777242 |
| microsatellite variability in peach [ prunus persica (l.) batsch]: cultivar identification, marker mutation, pedigree inferences and population structure. | a collection of 212 peach and nectarine cultivars covering a wide variation of the species were studied with 16 polymorphic single-locus microsatellite, or simple-sequence repeat (ssr), markers. the average number of alleles per locus was 7.3, 35% of the cultivar x locus combinations analyzed were heterozygous and 87% of the cultivars studied could be individually identified. most of the groups where two or more cultivars had the same ssr fingerprint included known peach mutants or possible syno ... | 2003 | 12750778 |
| characterization of a type ii chlorophyll a/b-binding protein gene (lhcb2*pp1) in peach. ii. mrna abundance in developing leaves exposed to sun or shade. | leaf development of shoots exposed to full sunlight and shoots shaded by the canopy was followed in field-grown, mature peach trees (prunus persica (l.) batsch, cv. loring) during the first half of the 1995 growing season. the architecture and size of shaded shoots and sun-exposed shoots differed significantly. total number of leaves produced on shaded shoots was significantly less than on sun-exposed shoots throughout the season, and differences in leaf number between light conditions increased ... | 2003 | 12670801 |
| purification and characterization of a beta-galactosidase from peach (prunus persica). | a beta-galactosidase (ec 3.2.1.23) from peach (prunus persica cv mibackdo) was purified and characterized. the purified peach beta-galactosidase was 42 kda in molecular mass and showed high enzyme activity against a the beta-galactosidase substrate, rho-nitrophenyl-beta-d-galactopyranoside. the km and vmax values of the enzyme activity of the peach beta-galactosidase were 5.16 and 0.19 mm for rho-nitrophenyl-beta-d-galactopyranoside mm/h, respectively. the optimum ph of the enzyme activity was p ... | 2003 | 12661763 |
| carbohydrate metabolism of vegetative and reproductive sinks in the late-maturing peach cultivar 'encore' | activities of nad(+)-dependent sorbitol dehydrogenase (sdh), sorbitol oxidase (sox), sucrose synthase (ss), acid invertase (ai), and neutral invertase (ni) in 'encore' peach (prunus persica l.) fruits and developing shoot tips were assayed during the growing season to determine whether carbohydrate metabolizing enzymes could serve as indicators of sink strength. in fruit flesh, ss activity was detected during stage i of growth, when cells were actively dividing, and sdh activity was detected dur ... | 1999 | 12651589 |
| influence of canopy light environment and nitrogen availability on leaf photosynthetic characteristics and photosynthetic nitrogen-use efficiency of field-grown nectarine trees. | relationships between co(2) assimilation at light saturation (a(max)), nitrogen (n) content and weight per unit area (w(a)) were studied in leaves grown with contrasting irradiances (outer canopy versus inner canopy) and n supply rates in field-grown nectarine trees prunus persica l. batsch. cv. fantasia. both a(max) and n content per unit leaf area (n(a)) were linearly correlated to w(a), but leaves in the high-n treatment had higher n(a) and a(max) for the same value of w(a) than leaves in the ... | 1999 | 12651580 |
| distribution of leaf mass per unit area and leaf nitrogen concentration determine partitioning of leaf nitrogen within tree canopies. | distribution of leaf nitrogen with respect to leaf mass per unit area (m(a)), nitrogen per unit mass (n(m)) and nitrogen per unit area (n(a)) within peach (prunus persica l.) tree canopies was studied in two field experiments. in one experiment, leaf light exposure and m(a) were measured on leaves from different canopy positions of peach trees subjected to five nitrogen (n) fertilization treatments. leaf light exposure and m(a) were linearly related and the relationship was independent of n fert ... | 2000 | 12651464 |
| storage of foliar-absorbed nitrogen and remobilization for spring growth in young nectarine (prunus persica var. nectarina) trees. | the effectiveness of spraying foliage with urea to provide nitrogen (n) to augment the seasonal internal cycling of n in young nectarine trees (prunus persica (l.) batsch var. nectarina (ait. f. maxim.), cv. stark red gold) was studied. one-year-old trees were grown with contrasting n supplies during the summer and foliage was sprayed with a 2% urea solution labeled with (15)n just before leaf senescence started. after leaf abscission had finished, the trees were repotted in sand and given no fu ... | 1998 | 12651390 |
| a carbon balance model of peach tree growth and development for studying the pruning response. | we modeled tree responses to pruning on the basis of growth rules established on unpruned trees and a simple principle governing root-shoot interactions. the model, which integrates architectural and ecophysiological approaches, distinguishes four types of anatomical organs in a tree: rootstock, main axis, secondary axes and new roots. tree structure is described by the position of secondary axes on the main axis. the main processes considered are plastochronal activity, branching, assimilate pr ... | 1998 | 12651360 |
| changes in fruit sugar concentrations in response to assimilate supply, metabolism and dilution: a modeling approach applied to peach fruit (prunus persica). | the influence of assimilate supply, metabolism and dilution on sugar concentrations in the mesocarp of peach (prunus persica (l.) batsch) fruit during the main stage of fruit enlargement was analyzed with the sugar model of génard and souty (1996). the model predicts the partitioning of carbon into sucrose, sorbitol, glucose and fructose in the mesocarp of peach fruit. based on measured data and the model, we determined values for the relative rates of sugar transformation. these rates were cons ... | 2003 | 12642239 |
| influence of branch autonomy on fruit, scaffold, trunk and root growth during stage iii of peach fruit development. | we studied the influence of branch autonomy on the growth of reproductive and vegetative organs by establishing different patterns of fruit distribution within and between large branch units (scaffolds) in mature peach trees (prunus persica (l.) batsch cv. 'elegant lady'). different patterns of fruit distribution were established by defruiting either whole scaffolds (uneven fruit distribution between scaffolds; us) or several selected hangers (small fruiting branches) per tree (uneven fruit dist ... | 2003 | 12615546 |
| construction of a bac library and its application to the identification of simple sequence repeats in peach [ prunus persica (l.) batsch]. | the rosaceae contains many economically important crop species, but their genomes are not well characterized, and comparative genetic mapping lags well behind that of other families. to facilitate genome comparisons and gene discovery in the rosaceae, we have begun the development of genomic resources for peach as the model genome for this family. first, we developed a simplified, cost-effective method for constructing bac libraries, particularly appropriate for plant species of relatively minor ... | 2002 | 12582893 |
| [immunofluorescence studies on the localization of integrin-like protein in plant pollen tubes]. | the strong fluorescence signals were obtained in pollen tube of lilium davidii duch with labeled anti-vnr integrin serum, and anti-beta 3, alpha v integrin subunit cytoplasmic domain serum separately. the highest density of immunolabel was in the tip of pollen tube. there was little or no immunolabel in control experiment using non-immune serum, second antibody alone and anti-fnr, lnr integrin serum separately. in pollen of prunus persica f. rubro-piena schneid, fluoresence signals were also obt ... | 1999 | 12548782 |
| genetic mapping of the evergrowing gene in peach [prunus persica (l.) batsch]. | in temperate locations, terminal apices on evergrowing (also called evergreen) peach trees keep growing in winter until killed by low temperatures, while the lateral buds go into dormancy. a recessive allele of a single gene (evergrowing or evg) controls this trait in peach. the amplified fragment length polymorphism (aflp) technique and bulked segregant analysis were applied to construct a local genetic linkage map for the evg gene from the cross empress op op dwarf x evergrowing (p.i. 442380). ... | 2004 | 12547924 |
| [stress effects of simulant acid rain on three woody plants]. | osmanthus fragrana, chimonanthus praecox and prunus persica were used as materials to investigate the effect of simulant acid rain on chlorophyll (chl) content, cell membrane permeability(l%), the content of proline (pro) and malondialdehyde (mda) in three woody plants with different resistance, and effects of the light and dark conditions on acid rain injury. the results showed that the change degree of four kinds of physiological and biochemical indexes for these woody plants was as sequence: ... | 2002 | 12533924 |
| responses of prunus ferganensis, prunus persica and two interspecific hybrids to moderate drought stress. | prunus ferganensis (kost. & riab) kov. & kost, a close relative of cultivated peach (prunus persica (l.) batsch.), is native to arid regions of central asia. a distinguishing feature of p. ferganensis is its prominent, elongated, unbranched pattern of leaf venation. to determine whether the long-vein trait could be used as a marker in breeding for drought tolerance, we investigated the association between this trait and the leaf morphological and physiological parameters related to drought respo ... | 2003 | 12511304 |
| the influence of severe shoot pruning on growth, carbon and nitrogen status in young peach trees (prunus persica). | one-year-old peach trees (prunus persica (l.) batsch) were severely pruned in july by removing 60% of the shoots. tree responses were analyzed in terms of architecture and nutritional status. tree growth was recorded from july to september by nondestructive (leaf production, thickening and branching of the remaining secondary axes) and destructive measurements (biomass partitioning and concentrations of total nitrogen (n) and nonstructural carbohydrates (nc) in specific tissues). the dry weights ... | 2002 | 12490426 |
| immunoassay to quantify the major peach allergen pru p 3 in foodstuffs. differential allergen release and stability under physiological conditions. | pru p 3 is a lipid transfer protein (ltp) that has been identified as the major peach (prunus persica) allergen. however, little is known about the amount present in both raw and processed foodstuffs. moreover, the in vivo release upon consumption of peach-containing foods remains unclear. we have developed a sensitive monoclonal antibody-based elisa for pru p 3. the method has been applied to measure the allergen levels in foodstuffs and the allergen release under different physiological condit ... | 2002 | 12475298 |
| contribution of vegetative storage proteins to seasonal nitrogen variations in the young shoots of peach trees (prunus persica l. batsch). | qualitative and quantitative variations in the level of two low molecular weight vegetative storage proteins (vsp 19 kda and 16.5 kda) in peach shoots were compared with annual variations in total nitrogen and total soluble proteins. protein patterns were obtained by sds-page and silver staining on each of the 12 kinetic samples collected between october 1995 and november 1996. vsp 16.5 kda and 19 kda exhibited typical annual vsp variations in both parenchyma and phloem. in wood, vsp 16.5 kda wa ... | 2002 | 12432035 |
| modulation of antioxidant compounds in organic vs conventional fruit (peach, prunus persica l., and pear, pyrus communis l.). | despite the increasing interest in organic products, knowledge about how different levels of fertilization affect nutritionally relevant components is still limited. the concentration of polyphenols and the activity of polyphenoloxidase (ppo), together with the content in ascorbic acid, citric acid, and alpha- and gamma-tocopherol, were assayed in conventional and organic peach (prunus persica l., cv. regina bianca) and pear (pyrus communis l., cv. williams). 2-thiobarbituric acid reactive subst ... | 2002 | 12207491 |
| characterization of the peach homologue of the ethylene receptor, ppetr1, reveals some unusual features regarding transcript processing. | to identify which processes in peach, prunus persica [l.] batsch., are associated with changes in ethylene perception, we cloned and characterized a peach homologue of the gene encoding the ethylene receptor, etr1. a fragment of the peach gene, ppetr1, obtained via pcr using degenerate primers against peach genomic dna was used to screen a cdna library made from ripening fruit. the resulting cdna and subsequent 3' race clones indicate that the ppetr1 coding region is highly similar to other etr1 ... | 2002 | 12172852 |
| gas exchange, biomass, whole-plant water-use efficiency and water uptake of peach (prunus persica) seedlings in response to elevated carbon dioxide concentration and water availability. | we examined the interactive effects of elevated co2 concentration ([co2]) and water stress on growth and physiology of 1-year-old peach (prunus persica l.) seedlings grown in 10-dm3 pots in open-top chambers with ambient (350 micromol mol-1) or elevated (700 micromol mol-1) [co2]. seedlings were supplied weekly with a non-limiting nutrient solution. water was withheld from half of the plants in each treatment for a 4-week drying cycle, to simulate a sudden and severe water stress during the phas ... | 2002 | 12091151 |