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purification of an anionic isoperoxidase from peach seeds and its immunological comparison with other anionic isoperoxidases.a soluble anionic isoperoxidase (ec 1,11,1,7) was purified from peach (prunus persica l. batsch cv. merry) seeds. purification was achieved by deae-sephacel, sephacryl s-300 and cm-cellulose chromatography. the purified isoperoxidase de-carboxylated indole-3-acetic acid (s(0.5) 0.13 mm, hill coefficient 1.7). molecular mass, determined by gel filtration and sodium dodecyl sulfate polyacrylamide gel electrophoresis, was ca 60 kda. polyclonal antibodies were raised in rabbit against this isoperoxi ...199021087270
traditional remedies used for the treatment of cattle wounds and myiasis in amatola basin, eastern cape province, south africa.a questionnaire survey was carried out to document traditional remedies used in the treatment of cattle wounds and myiasis in amatola basin. eighty-three cattle farmers from nine villages were interviewed. the majority of respondents (67.9%) use traditional remedies for treatment of these conditions. however, 25.9% of them use a combination of traditional remedies with conventional medicines. the survey revealed that 13 medicinal plants belonging to 11 families were used. aloe ferox, prunus pers ...200921344789
attraction of phlebotomus papatasi to common fruit in the field.sand flies have been reported feeding on various plant organs including stems, leaves, and flowers but the attraction of sand flies to sugar-rich fruits has received little attention. in this study, we tested 24 commercially available fruits for their attractiveness to sand flies, and found that the top three attractive fruits were nectarine (prunus persica var. nectarina), cactus fruit, (opuntia ficus-indica), and guava (psidium guajava). these fruits were fed upon equally by both males and fem ...201121366776
how reliable is the double-ended pressure sleeve technique for assessing xylem vulnerability to cavitation in woody angiosperms?the reliability of a double-ended pressure sleeve technique was evaluated on three woody angiosperm species with contrasting maximum vessel lengths. vulnerability curves (vcs) were constructed by varying sample length and the size of the pressure sleeves. vcs were compared against curves obtained with reference techniques. for the two diffuse-porous species, betula pendula and prunus persica, vcs built with shoot segments shorter than maximum vessel length strongly overestimated species vulnerab ...201121401617
mutation scanning of peach floral genes.abstract: background: mutation scanning technology has been used to develop crop species with improved traits. modifications that improve screening throughput and sensitivity would facilitate the targeted mutation breeding of crops. technical innovations for high-resolution melting (hrm) analysis are enabling the clinic-based screening for human disease gene polymorphism. we examined the application of two hrm modifications, cold-pcr and qmc-pcr, to the mutation scanning of genes in peach, prunu ...201121605436
phenotypic plasticity of stem water potential correlates with crop load in horticultural trees.conceptual models accounting for the influence of source:sink ratio on water relations of trees are theoretically relevant from a physiological perspective and practically important for irrigation scheduling. midday stem water potential of horticultural trees often declines with increasing crop load but the actual response depends on environmental, management and plant factors. here we advance a quantitative synthesis of the response of stem water potential to crop load from the perspective of p ...201121636690
screening of estrogenic and antiestrogenic activities from medicinal plants.the medicinal plant extracts commercially used in asia were screened for their estrogenic and antiestrogenic activities in a recombinant yeast system featuring both a human estrogen receptor (er) expression plasmid and a reporter plasmid. pueraria lobata (flower) had the highest estrogenic relative potency (rp, 7.75+ù10(-3); rp of 17+¦-estradiol=1), followed by amomum xanthioides (1.25+ù10(-3)). next potent were a group consisting of glycyrrhiza uralensis, zingiber officinale, rheum undulatum, c ...200821783839
Monilinia species causing brown rot of peach in China.In this study, 145 peaches and nectarines displaying typical brown rot symptoms were collected from multiple provinces in China. A subsample of 26 single-spore isolates were characterized phylogenetically and morphologically to ascertain species. Phylogenetic analysis of internal transcribed spacer (ITS) regions 1 and 2, glyceraldehyde-3-phosphate dehydrogenase (G3PDH), ß-tubulin (TUB2) revealed the presence of three distinct Monilinia species. These species included Monilinia fructicola, Monili ...201121980371
the cucumber vein yellowing virus silencing suppressor p1b can functionally replace hcpro in plum pox virus infection in a host-specific manner.plant viruses of the genera potyvirus and ipomovirus (potyviridae family) use unrelated rna silencing suppressors (rss) to counteract antiviral rna silencing responses. hcpro is the rss of potyvirus spp., and its activity is enhanced by the upstream p1 protein. distinctively, the ipomovirus cucumber vein yellowing virus (cvyv) lacks hcpro but contains two p1 copies in tandem (p1ap1b), the second of which functions as rss. using chimeras based on the potyvirus plum pox virus (ppv), we found that ...201121970691
molecular characterization of peach pr genes and their induction kinetics in response to bacterial infection and signaling molecules.'venture' and 'babygold 5' are two peach cultivars with a demonstrated resistance and susceptibility, respectively, to bacterial spot disease caused by xanthomonas campestris pv. pruni (xcp). to explore the differences between these cultivars at the molecular level, two pr1 (pp-pr1a, pp-pr1b) and three pr5 (pp-tlp1, pp-tlp2 and pp-tlp3) genes were isolated from peach (prunus persica l.) and investigated by in silico and in situ approaches. the analysis o ...201122101723
assessment of prunus persica fruit softening using a proteomics approach.fruit ripening in prunus persica involves a number of physiological changes, being one of the most significant the mesocarp softening in melting varieties. in order to get a better understanding of the molecular processes involved in this phenomenon, the protein accumulation patterns in firm and soft fruit of three peach and two nectarine melting flesh varieties were assessed using 2d gel analysis. a general linear model (glm) two-way analysis of variance determined that 164 of the 621 protein s ...201122178302
effects of dietary fruits, vegetables and a herbal tea on the in vitro transport of cimetidine: comparing the caco-2 model with porcine jejunum tissue.context: dietary botanicals are often consumed together with allopathic medicines, which may give rise to pharmacokinetic interactions. in vitro intestinal models are useful to identify botanical-drug interactions, but they may exhibit different expressions of transporters or enzymes. objective: to compare the effects of selected dietary botanical extracts on cimetidine transport across two in vitro intestinal models. materials and methods: bi-directional transport of cimetidine was measured ...201122085278
Interactions Between Extrafloral Nectaries, Ants (Hymenoptera: Formicidae), and Other Natural Enemies Affect Biological Control of Grapholita molesta (Lepidoptera: Tortricidae) on Peach (Rosales: Rosaceae).Extrafloral nectaries (EFNs) are reported to benefit some plants when ants (Hymenoptera: Formicidae) use their secretions and fend off herbivores, but in some cases resulting competitive interactions may reduce biological control of specific herbivores. This research examined the interactions between ants and other natural enemies associated with the EFNs of peach [Prunus persica (L.) Batcsh] and the implications for biological control of a key pest, the oriental fruit moth [Grapholita molesta ...201122182610
lesser peachtree borer (lepidoptera: sesiidae) oviposition on prunus germplasm.the lesser peachtree borer, synanthedon pictipes (grote and robinson) (lepidoptera: sesiidae), is a serious pest of peach, prunus persica (l.) batsch, across the southeastern united states. we examined oviposition by s. pictipes on field-grown prunus scion and rootstock cultivars and two endemic prunus spp. when sawn limbs, not roots, were assayed in the laboratory. a choice test compared oviposition on the peach scion 'harvester', peach rootstock 'guardian', plum × peach hybrid rootstock 'mp-2 ...201122217762
association between chloroplast and mitochondrial dna sequences in chinese prunus genotypes (prunus persica, prunus domestica, and prunus avium).the nuclear dna is conventionally used to assess the diversity and relatedness among different species, but variations at the dna genome level has also been used to study the relationship among different organisms. in most species, mitochondrial and chloroplast genomes are inherited maternally; therefore it is anticipated that organelle dna remains completely associated. many research studies were conducted simultaneously on organelle genome. the objectives of this study was to analyze the genet ...201525592231
a remarkable synergistic effect at the transcriptomic level in peach fruits doubly infected by prunus necrotic ringspot virus and peach latent mosaic viroid.microarray profiling is a powerful technique to investigate expression changes of large amounts of genes in response to specific environmental conditions. the majority of the studies investigating gene expression changes in virus-infected plants are limited to interactions between a virus and a model host plant, which usually is arabidopsis thaliana or nicotiana benthamiana. in the present work, we performed microarray profiling to explore changes in the expression profile of field-grown prunus ...201323710752
genome wide identification of chilling responsive micrornas in prunus persica.micrornas (mirnas) are small rnas (srnas) approximately 21 nucleotides in length that negatively control gene expression by cleaving or inhibiting the translation of target gene transcripts. within this context, mirnas and sirnas are coming to the forefront as molecular mediators of gene regulation in plant responses to annual temperature cycling and cold stress. for this reason, we chose to identify and characterize the conserved and non-conserved mirna component of peach (prunus persica (l.) b ...201222978558
draft genome sequence of pseudomonas syringae pv. persicae ncppb 2254.pseudomonas syringae pv. persicae is a pathogen that causes bacterial decline of stone fruit. here, we report the draft genome sequence for p. syringae pv. persicae, which was isolated from prunus persica.201526044420
gene expression changes during the gummosis development of peach shoots in response to lasiodiplodia theobromae infection using rna-seq.lasiodiplodia theobromae is a causal agent of peach (prunus persica l.) tree gummosis, a serious disease affecting peach cultivation and production. however, the molecular mechanism underlying the pathogenesis remains unclear. rna-seq was performed to investigate gene expression in peach shoots inoculated or mock-inoculated with l. theobromae. a total of 20772 genes were detected in eight samples; 4231, 3750, 3453, and 3612 differentially expressed genes were identified at 12, 24, 48, and 60 h a ...201627242544
tnl genes in peach: insights into the post-lrr domain.plants develop sustainable defence responses to pathogen attacks through resistance (r) genes contributing to effector-triggered immunity (eti). tir-nb-lrr genes (tnl genes) constitute a major family of eti r genes in dicots. the putative functions or roles of the tir, nb and lrr domains of the proteins they encode (tnls) are well documented, but tnls also have a poorly characterised c-terminal region, the function of which is unknown in most cases. we characterised this prevalent stress-respons ...201627129402
identification of proteins from prunus persica that interact with peach latent mosaic viroid.peach latent mosaic viroid (plmvd) is a small, single-stranded, circular rna pathogen that infects prunus persica trees. as with all other known viroids, the plmvd genome does not encode any proteins. consequently, it must interact with host cellular factors in order to ensure its life cycle. with the objective of identifying cellular proteins that interact with plmvd, northwestern hybridizations were performed using partially purified peach leaf extracts. mass spectrometric analysis of the dete ...200919759139
archaeological evidence for peach (prunus persica) cultivation and domestication in china.the cultivated/domesticated peach (prunus persica var. persica; rosaceae, subgenus amygdalus; synonym: amygdalus persica) originated in china, but its wild ancestor, as well as where, when, and under what circumstances the peach was domesticated, is poorly known. five populations of archaeological peach stones recovered from zhejiang province, china, document peach use and evolution beginning ca. 8000 bp. the majority of the archaeological sites from which the earliest peach stones have been rec ...201425192436
peach (prunus persica l.).until now, the application of genetic transformation techniques in peach has been limited by the difficulties in developing efficient regeneration and transformation protocols. here we describe an efficient regeneration protocol for the commercial micropropagation of gf677 rootstock (prunus persica × prunus amygdalus). the method is based on the production, via organogenesis, of meristematic bulk tissues characterized by a high competence for shoot regeneration. this protocol has also been used ...201525416260
characterization and comparison of the cpk gene family in the apple (malus × domestica) and other rosaceae species and its response to alternaria alternata infection.as one of the ca2+ sensors, calcium-dependent protein kinase (cpk) plays vital roles in immune and stress signaling, growth and development, and hormone responses, etc. recently, the whole genome of apple (malus × domestica), pear (pyrus communis), peach (prunus persica), plum (prunus mume) and strawberry (fragaria vesca) in rosaceae family has been fully sequenced. however, little is known about the cpk gene family in these rosaceae species. in this study, 123 cpk genes were identified from fiv ...201627186637
antibacterial, antifungal and antioxidant activities of honey collected from timergara (dir, pakistan).in this study honeys of acacia modesta, prunus persica, zizyphus sativa and isodon rogosus plants were tested against two gram-positive bacterial strains (staphylococcus aureus and bacillus cereus), two gram-negative bacterial strains (klebsilla pneumonia and escherichia coli) and two fungal strains (alternaria alternata and trichoderma harzianum) through agar well diffusion method. the tested honeys showed high antimicrobial activities to the tested bacterial and fungal strains. all the tested ...201424374434
complete nucleotide sequences of the genomes of two isolates of apple chlorotic leaf spot virus from peach (prunus persica) in china.the complete nucleotide sequences of two isolates of apple chlorotic leaf spot virus (z1 and z3) collected from peach in henan province, china, were determined. the genomes of both z1 and z3 were found to contain three open reading frames (orfs). sequence analysis showed that genomic sequences of z1 and z3 isolates shared 67.4%-82.9% and 67.2%-82.6% identity, respectively, with the other eight isolates of aclsv that have been reported previously. based on the putative amino acid sequences of the ...201222278708
bioactive compounds contents, antioxidant and antimicrobial activities during ripening of prunus persica l. varieties from the north west of tunisia.bioactive molecules from fruits of four varieties of prunus persica at different stages of ripening (green, small orange, red) were studied. for example, contents on polyphenols (20.36mg gae/g fw) and flavonoids (0.764mg re/g fw) were high and varied according variety. the antioxidant activity, using four different tests (dpph radical scavenging activity, reducing power, β carotene bleaching system and tbars assay) showed that the variety chatos exhibited the highest antioxidant activity compari ...201626988472
complete nucleotide sequences of two isolates of cherry green ring mottle virus from peach (prunus persica) in china.two complete nucleotide sequences of cherry green ring mottle virus (cgrmv) isolated from peach in hebei (hs10) and fujian (f9) provinces, china, were determined. five open reading frames (orfs) were found in the genomes of both isolates. the f9 and hs10 isolates shared 82.2 % and 83.4-94.4 % nucleotide sequence identity, respectively, with two cgrmv isolates from cherry. analysis of the nucleotide and amino acid sequences from the five orfs of both isolates showed that hs10 shares the greatest ...201323108577
genome-wide identification, expression patterns, and functional analysis of udp glycosyltransferase family in peach (prunus persica l. batsch).peach (prunus persica l. batsch) is a commercial grown fruit trees, important because of its essential nutrients and flavor promoting secondary metabolites. the glycosylation processes mediated by udp-glycosyltransferases (ugts) play an important role in regulating secondary metabolites availability. identification and characterization of peach ugts is therefore a research priority. a total of 168 peach ugt genes that distributed unevenly across chromosomes were identified based on their conserv ...201728382047
glucitol dehydrogenase from peach (prunus persica) fruits is regulated by thioredoxin h.glucitol (gol) is a major photosynthetic product in plants from the rosaceae family. herein we report the molecular cloning, heterologous expression and characterization of gol dehydrogenase (goldhase, ec 1.1.1.14) from peach (prunus persica) fruits. the recombinant enzyme showed kinetic parameters similar to those reported for orthologous enzymes purified from apple and pear fruits. the activity of recombinant goldhase was strongly inhibited by cu(2+) and hg(2+), suggesting that it might have c ...201424747954
carbohydrate metabolism changes in prunus persica gummosis infected with lasiodiplodia theobromae.peach gummosis represents a significant global disease of stone fruit trees and a major disease in the south peach production area of the yangtze river of china. in this study, the carbohydrate composition of peach shoots during infection by lasiodiplodia theobromae was examined. the expression of genes related to metabolic enzymes was also investigated. control wounded and noninoculated tissue, lesion tissue, and wounded and inoculated surrounding lesion tissue of peach shoots were analyzed. so ...201424283537
evidence of differences between the communities of arbuscular mycorrhizal fungi colonizing galls and roots of prunus persica infected by the root-knot nematode meloidogyne incognita.arbuscular mycorrhizal fungi (amf) play important roles as plant protection agents, reducing or suppressing nematode colonization. however, it has never been investigated whether the galls produced in roots by nematode infection are colonized by amf. this study tested whether galls produced by meloidogyne incognita infection in prunus persica roots are colonized by amf. we also determined the changes in amf composition and biodiversity mediated by infection with this root-knot nematode. dna from ...201121984233
reproduction and development of meloidogyne incognita and m. javanica on guardian peach rootstock.guardian peach rootstock was evaluated for susceptibility to meloidogyne incognita race 3 (georgia-peach isolate) and m. javanica in the greenhouse. both commercial guardian seed sources produced plants that were poor hosts of m. incognita and m. javanica. reproduction as measured by number of egg masses and eggs per plant, eggs per egg mass, and eggs per gram of root were a better measure of host resistance than number of root galls per plant. penetration, development, and reproduction of m. in ...199919270905
impact of meloidogyne incognita on the incidence of peach tree short life in the presence of criconemella xenoplax.the relationship between cricenemella xenoplax alone and in combination with meloidogyne incognitaon the incidence of peach tree short life disease was studied in field microplots during 1989-96. the presence of m. incognita suppressed the population density of c. xenoplax on lovell peach. tree trunk diameter was significantly reduced in the presence of both nematode species prior to 1993. soil ph was lowest in the co-infection treatment as compared with the uninoculated control on three of the ...199719274276
optimal levels of meloidogyne incognita inoculum for infection of tomato and peach in vitro.penetration of second-stage juveniles (j2) of meloidogyne incognita into tomato root explants and in vitro propagated peach plantlet roots were compared. five inoculum levels were used: 25, 50, 75, 100, and 200 j2 for tomato; and 50, 100, 200, 500, and 1,000j2 for peach. the greatest root penetration into tomato was 30% at the 75 j2 level, but the maximum penetration into peach roots was only 8% at the 200 j2 level. the difference (p = 0.05) in penetration of m. incognita at all inoculum levels ...199419279926
dynamics of concomitant populations of meloidogyne incognita and criconemella xenoplax on peach.the interaction between meloidogyne incognita and criconemella xenoplax on nematode reproduction and growth of lovell peach was studied in field microlots and the greenhouse. meloidogyne incognita suppressed reproduction of c. xenoplax in both field and greenhouse experiments. tree growth, as measured by trunk diameter, was reduced (p </= 0.05) in the presence of m. incognita as compared with c. xenoplax of the uninoculated control trees 26 months following inoculation. a similar response regard ...199319279823
response of peach scion cultivars and rootstocks to meloidogyne incognitain vitro and in microplots.the response of the peach scion cultivars, jerseyqueen, redhaven, compact redhaven, and rio oso gem and rootstocks 'lovely and 'nemaguard' to inoculation with meloidogyne incognita was compared in vitro and in microplots. one or more parameters monitored in vitro correlated with at least one parameter monitored in microplots, 4 years after tree planting (1989). a range of responses was observed from highlysusceptible in lovell to resistant in nemaguard. in vitro and microplot data suggest high a ...199319279797
establishment of orchards with black polyethylene film mulching: effect on nematode and fungal pathogens, water conservation, and tree growth.placement of a 3-m-wide, black, polyethylene film mulch down rows of peach (prunus persica 'red haven' on 'lovell' rootstock) and almond (prunus dulcis 'nonpareil' on 'lovell') trees in the san joaquin valley of california resulted in irrigation water conservation of 75%, higher soil temperature in the surface 30 cm, a tendency toward greater root mass, elimination of weeds, and a greater abundance of meloidogyne incognita second-stage juveniles in soil but reduced root galling when compared to ...199219283045
high-throughput targeted ssr marker development in peach (prunus persica).simple sequence repeats (ssrs) have proven to be highly polymorphic, easily reproducible, codominant markers. however, developing an ssr map is very time consuming and expensive, and most ssrs are not specifically linked to gene loci of immediate interest. the ideal situation would be to combine a high-throughput, relatively inexpensive mapping technique with rapid identification of ssr loci in mapped regions of interest. for this reason, we coupled the high-throughput technique of aflp mapping ...200211962629
populations of criconemella xenoplax on peach interplanted with certain herbaceous plants.peach trees growing in field microplots infested with criconemella xenoplax were interplanted with centipedegrass, goosegrass, sicklepod, marigold, buckhorn plantain, showy crotalaria, or perennial ryegrass, or left with weed-free soil as the control. buckhorn plantain reduced nematode numbers, but not to levels acceptable for commercial control. marigold, sicklepod, and goosegrass reduced nematode numbers on some sampling dates but not on others. other plants had no effect or appeared to stimul ...199219283046
influence of criconemella xenoplax and pruning time on short life of peach trees.influences of criconemella xenoplax and pruning dates were studied in field microplots with 'nemaguard' peach cuttings on a site not previously planted to peaches. trees with or without c. xenoplax were pruned beginning in december 1984 or march 1985. peach tree short life (ptsl) did not occur in the absence of c. xenoplax. ptsl occurred earlier in december-pruned than in march-pruned inoculated trees. results confirm that "old" peach sites are not required for ptsl to occur. pruning nemaguard a ...199019287694
peach leaf senescence delayed by criconemella xenoplax.fall annual leaf senescence of peach was delayed in the field and in microplots in the presence of criconemella xenoplax. soil from the rhizosphere of orchard trees with greener leaves had ca. 2.5 x more nematodes than soil around trees in a more advanced state of fall senescence. in microplots, monoclonal antibody enzyme immunoassay (eia) of leaf cytokinins indicated that concentration of zeatin riboside-like substances and chlorophyll content were greater in leaves of trees growing in nematode ...198819290258
behavior, parasitism, morphology, and biochemistry of criconemella xenoplax and c. ornata on peach.host-parasite relationships of criconemella xenoplax and c. ornata on nemaguard peach and common bermudagrass were determined in the greenhouse. criconemella xenoplax reproduced on peach and reduced root volume, height, and dry stem weight after 6 months, compared with the noninfested check. numbers of c. ornata did not increase on peach or influence peach growth, but they did reduce dry top weight and root volume of common bermudagrass, compared with c. xenoplax. criconemella xenoplax and c. or ...198819290183
effect of initial population density of criconemella xenoplax on reducing sugars, free amino acids, and survival of peach seedlings over time.percentage of mortality, growth suppression, and changes in free amino acid and reducing sugar content in root and (or) stem tissue of nemaguard peach seedlings were studied in the greenhouse in relation to time and eight different initial population densities (pi) of criconemella xenoplax. after 90 and 180 days, free amino acid content in root tissue significantly increased with increasing nematode numbers. suppression of root volume, dry root and stem weight, height increase, plant survival, a ...198719290147
small rnas containing the pathogenic determinant of a chloroplast-replicating viroid guide the degradation of a host mrna as predicted by rna silencing.how viroids, tiny non-protein-coding rnas (~250-400 nt), incite disease is unclear. one hypothesis is that viroid-derived small rnas (vd-srnas; 21-24 nt) resulting from the host defensive response, via rna silencing, may target for cleavage cell mrnas and trigger a signal cascade, eventually leading to symptoms. peach latent mosaic viroid (plmvd), a chloroplast-replicating viroid, is particularly appropriate to tackle this question because it induces an albinism (peach calico, pc) strictly assoc ...201222332758
a viroid rna with a specific structural motif inhibits chloroplast development.peach latent mosaic viroid (plmvd) is a chloroplast-replicating rna that propagates in its natural host, peach (prunus persica), as a complex mixture of variants, some of which are endowed with specific structural and pathogenic properties. this is the case of variant pc-c40, with an insertion of 12 to 13 nucleotides that folds into a hairpin capped by a u-rich loop, which is responsible for an albino-variegated phenotype known as peach calico (pc). we have applied a combination of ultrastructur ...200718055612
fighting sharka in peach: current limitations and future perspectives.sharka, caused by plum pox virus (ppv), is by far the most important infectious disease of peach [p. persica (l.) batsch] and other prunus species. the progressive spread of the virus in many important growing areas throughout europe poses serious issues to the economic sustainability of stone fruit crops, peach in particular. the adoption of internationally agreed-upon rules for diagnostic tests, strain-specific monitoring schemes and spatial-temporal modeling of virus spread, are all essential ...201627625664
genome-wide association links candidate genes to resistance to plum pox virus in apricot (prunus armeniaca).in fruit tree species, many important traits have been characterized genetically by using single-family descent mapping in progenies segregating for the traits. however, most mapped loci have not been sufficiently resolved to the individual genes due to insufficient progeny sizes for high resolution mapping and the previous lack of whole-genome sequence resources of the study species. to address this problem for plum pox virus (ppv) candidate resistance gene identification in prunus species, we ...201626356603
diverse amino acid changes at specific positions in the n-terminal region of the coat protein allow plum pox virus to adapt to new hosts.plum pox virus (ppv)-d and ppv-r are two isolates from strain d of ppv that differ in host specificity. previous analyses of chimeras originating from ppv-r and ppv-d suggested that the n terminus of the coat protein (cp) includes host-specific pathogenicity determinants. here, these determinants were mapped precisely by analyzing the infectivity in herbaceous and woody species of chimeras containing a fragment of the 3' region of ppv-d (including the region coding for the cp) in a ppv-r backbon ...201323745677
o-glcnac modification of the coat protein of the potyvirus plum pox virus enhances viral infection.o-glcnacylation is a dynamic protein modification which has been studied mainly in metazoans. we reported previously that an arabidopsis thaliana o-glcnac transferase modifies at least two threonine residues of the plum pox virus (ppv) capsid protein (cp). now, six additional residues were shown to be involved in o-glcnac modification of ppv cp. cp o-glcnacylation was abolished in the ppv cp7-t/a mutant, in which seven threonines were mutated. ppv cp7-t/a infected nicotiana clevelandii, nicotian ...201323639873
plant growth stimulation in prunus species plantlets by bth or otc treatments under in vitro conditions.the effects of benzothiadiazole (bth) and l-2-oxothiazolidine-4-carboxylic acid (otc) on the growth and viral content of micropropagated, plum pox virus (ppv)-infected peach [(prunus persica (l.) batsch] 'gf305' plantlets were analyzed. low bth and otc concentrations resulted in a significant increase in the growth of gf305 peach and plum plants, with greater effects in ppv-infected than in healthy gf305 peach plantlets. neither bth nor otc reduced the virus content. in fact, the highest growth ...201222595304
virus variants with differences in the p1 protein coexist in a plum pox virus population and display particular host-dependent pathogenicity features.subisolates segregated from an m-type plum pox virus (ppv) isolate, ppv-ps, differ widely in pathogenicity despite their high degree of sequence similarity. a single amino acid substitution, k109e, in the helper component proteinase (hcpro) protein of ppv caused a significant enhancement of symptom severity in herbaceous hosts, and notably modified virus infectivity in peach seedlings. the presence of this substitution in certain subisolates that induced mild symptoms in herbaceous hosts and did ...201222458641
pperf3b, a transcriptional repressor from peach, contributes to disease susceptibility and side branching in ear-dependent and -independent fashions.peach erf3b is a potent transcriptional repressor for defense-related genes even in the presence of similar levels of transcriptional activators and can interfere with plant development through pathways independent of the ear motif. ethylene response factors (erfs) are a major group of plant transcription factors with either activation or repression capabilities on gene transcription. repressor-type erfs are characterised by an intrinsic motif, namely the erf-associated amphiphilic repression mo ...201323515898
dual regulation of water retention and cell growth by a stress-associated protein (sap) gene in prunus.we have identified a gene (ppsap1) of prunus persica coding for a stress-associated protein (sap) containing zn-finger domains a20 and an1. saps have been described as regulators of the abiotic stress response in plant species, emerging as potential candidates for improvement of stress tolerance in plants. ppsap1 was highly expressed in leaves and dormant buds, being down-regulated before bud dormancy release. ppsap1 expression was moderately induced by water stresses and heat in buds. in additi ...201728336950
unraveling early events in the taphrina deformans - prunus persica interaction: an insight into the differential responses in resistant and susceptible genotypes.leaf peach curl is a devastating disease affecting leaves, flowers and fruits caused by the dimorphic fungus taphrina deformans. to gain insight into the mechanisms of fungus pathogenesis and plant responses, leaves of a resistant and two susceptible p. persica genotypes were inoculated with blastospores (yeast), and the infection was monitored during 120 hpi. fungal dimorphism to the filamentous form and induction of ros, callose synthesis, cell death, and defense compound production was observ ...201728244594
expression of peach sucrose transporters in heterologous systems points out their different physiological role.sucrose is the major phloem-translocated component in a number of economically important plant species. the comprehension of the mechanisms involved in sucrose transport in peach fruit appears particularly relevant, since the accumulation of this sugar, during ripening, is crucial for the growth and quality of the fruit. here, we report the functional characterisation and subcellular localisation of three sucrose transporters (ppsut1, ppsut2, ppsut4) in peach, and we formulate novel hypotheses a ...201526259193
the cloning and functional characterization of peach constans and flowering locus t homologous genes ppco and ppft.flowering is an essential stage of plant growth and development. the successful transition to flowering not only ensures the completion of plant life cycles, it also serves as the basis for the production of economically important seeds and fruits. constans (co) and flowering locus t (ft) are two genes playing critical roles in flowering time control in arabidopsis. through homology-based cloning and rapid-amplifications of cdna ends (race), we obtained full-lengths cdna sequences of prunus pers ...201525905637
tylenchulus palustris parasitizing peach trees in the of nited states.most morphological characteristics of three populations of a tylenchulus sp. from peach roots in alabama, arkansas, and georgia did not differ from those of t. palustris paratypes. however, some mature females differed slightly from those of t. palustris paratypes from florida. these mature females were more swollen in the posterior portion of their bodies, and they possessed digitate postvulval body sections with round rather than conoid termini. these morphological variants had a wide postvulv ...199019287688
draft genome sequence of two strains of xanthomonas arboricola isolated from prunus persica which are dissimilar to strains that cause bacterial spot disease on prunus spp.the draft genome sequences of two strains of xanthomonas arboricola, isolated from asymptomatic peach trees in spain, are reported here. these strains are avirulent and do not belong to the same phylogroup as x. arboricola pv. pruni, a causal agent of bacterial spot disease of stone fruits and almonds.201627609931
plant-parasitic nematode communities in dogwood, maple, and peach nurseries in tennessee.nursery blocks (48 dogwood, 27 red maple, and 17 peach) distributed among 20 tennessee nurseries were sampled for nematodes in march, july, and october 1981. plant-parasitic nematodes were extracted from soil, counted by genera, and identified to species after fixation. a total of 57 species in 24 genera were found, with 1-16 species occurring in a site. the species most commonly detected were paratylenchus projectus and xiphinema americanum, which were found in 88% and 78% of the sites, respect ...198519294071
nematode community structure in dogwood, maple, and peach nurseries in tennessee.nursery blocks (48 dogwood, 27 maple, 17 peach) in 20 middle tennessee nurseries were sampled for nematodes in march,july, and october 1981. dogwoods and maples were grouped in three age classes: 1-2, 3-5, and 10+ years. nematodes were extracted from soil samples, counted, and assigned to trophic groups as follows: plant parasites, microbivores, fungivores, predators, and omnivores (= dorylaimida). total nematode numbers per 200 cm s soil ranged from 52 to 9,166 (mean = 1,785 +/- 1,420). nematod ...198519294070
ethnomedicinal plants used by the villagers of district udhampur, j&k, india.plants are an integral part of life in many indigenous communities. besides, being the source of food, fodder, fuel, etc., the use of plants as herbal medicines in curing several ailments goes parallel to the human civilization. ethnopharmacology involves the investigation of the plants used by the traditional communities and further understand the pharmacological basis of these culturally important medicinal plants. present study was conducted to enlist the medicinal plants used by the local in ...201424365639
host plant effects on halyomorpha halys (hemiptera: pentatomidae) nymphal development and survivorship.halyomorpha halys(stål) (hemiptera: pentatomidae) is a highly polyphagous invasive species and an important pest of orchard crops in the united states. in the mid-atlantic region, wild hosts ofh. halysare common in woodlands that often border orchards, andh. halysmovement from them into orchards poses ongoing management issues. to improve our understanding of host plant effects onh. halyspopulations at the orchard-woodland interface, nymphal survivorship, developmental duration, and adult fitnes ...201627012749
dynamic of plant composition and regeneration following windthrow in a temperate beech forest.the effects of soil pedoturbation (i.e., pit and mound microtopography, pm) on development of herbaceous plant species and woody species regeneration were examined in a temperate beech forest (fagus orientalis lipsky) in northern iran. we recorded the vegetation in 20 pairs of disturbed and adjacent undisturbed plots and established a chronosequence of pm ages to study the effect of time since microsite formation on cover percent of herbaceous plants and woody regeneration status. according to o ...201427379260
the association between chromaticity, phenolics, carotenoids, and in vitro antioxidant activity of frozen fruit pulp in brazil: an application of chemometrics.a total of 19 brazilian frozen pulps from the following fruits: açai (euterpe oleracea), blackberry (rubus sp.), cajá (spondias mombin), cashew (anacardium occidentale), cocoa (theobroma cacao), coconut (cocos nucifera), grape (vitis sp.), graviola (annona muricata), guava (psidium guajava), papaya (carica papaya), peach (prunus persica), pineapple (ananas comosus), pineapple and mint (a. comosus and mentha spicata), red fruits (rubus sp. and fragaria sp.), seriguela (spondias purpurea), strawbe ...201424547813
de novo assembly and characterization of transcriptomes of early-stage fruit from two genotypes of annona squamosa l. with contrast in seed number.annona squamosa l., a popular fruit tree, is the most widely cultivated species of the genus annona. the lack of transcriptomic and genomic information limits the scope of genome investigations in this important shrub. it bears aggregate fruits with numerous seeds. a few rare accessions with very few seeds have been reported for annona. a massive pyrosequencing (roche, 454 gs flx+) of transcriptome from early stages of fruit development (0, 4, 8 and 12 days after pollination) was performed to pr ...201525766098
managed bumblebees outperform honeybees in increasing peach fruit set in china: different limiting processes with different pollinators.peach prunus persica (l.) batsch is self-compatible and largely self-fertile, but under greenhouse conditions pollinators must be introduced to achieve good fruit set and quality. because little work has been done to assess the effectiveness of different pollinators on peach trees under greenhouse conditions, we studied 'okubo' peach in greenhouse tunnels near beijing between 2012 and 2014. we measured pollen deposition, pollen-tube growth rates, ovary development, and initial fruit set after th ...201525799170
subcellular concentrations of sugar alcohols and sugars in relation to phloem translocation in plantago major, plantago maritima, prunus persica, and apium graveolens.sugar and sugar alcohol concentrations were analyzed in subcellular compartments of mesophyll cells, in the apoplast, and in the phloem sap of leaves of plantago major (common plantain), plantago maritima (sea plantain), prunus persica (peach) and apium graveolens (celery). in addition to sucrose, common plantain, sea plantain, and peach also translocated substantial amounts of sorbitol, whereas celery translocated mannitol as well. sucrose was always present in vacuole and cytosol of mesophyll ...200818188589
isolation and characterization of soluble boron complexes in higher plants. the mechanism of phloem mobility of boron.boron (b) polyol complexes have been isolated and characterized from the phloem sap of celery (apium graveolens l.) and the extrafloral nectar of peach (prunus persica l.). in celery the direct analysis of untreated phloem sap by matrix-assisted laser desorption-fourier transform mass spectrometry, with verification by high-performance liquid chromagraphy and gas chromatography-mass spectrometry, revealed that b is present in the phloem as the mannitol-b-mannitol complex. molecular modeling furt ...19979046600
a snare-like superfamily protein sbslsp from the halophyte salicornia brachiata confers salt and drought tolerance by maintaining membrane stability, k(+)/na(+) ratio, and antioxidant machinery.about 1000 salt-responsive ests were identified from an extreme halophyte salicornia brachiata. among these, a novel salt-inducible gene sbslsp (salicornia brachiata snare-like superfamily protein), showed up-regulation upon salinity and dehydration stress. the presence of cis-regulatory motifs related to abiotic stress in the putative promoter region supports our finding that sbslsp gene is inducible by abiotic stress. the sbslsp protein showed a high sequence identity to hypothetical/uncharact ...201627313584
molecular cloning and expression analyses of rps3a gene from mulberry under abiotic stresses and among different mulberry varieties.a full-length cdna sequence coding ribosomal protein s3a of mulberry tree, which we designated mmrps3a (genbank accession no. kr610331), was cloned based on mulberry expressed sequence tags. sequence analysis showed that the mmrps3a is 1089 bp long and contains a 80-bp 5'-utr (untranslated region) and a 220-bp 3'-utr. its open reading frame consists of a 789-bp encoding 262 amino acids with a predicted molecular weight of 30.053 kda and an isoelectric point of 9.84. homology analysis revealed th ...201627173298
occupational allergy to peach (prunus persica) tree pollen and potential cross-reactivity between rosaceae family pollens.orchard workers in north china are highly exposed to orchard pollens, especially peach and other rosaceae family pollens during pollination season. the aim of this study was to investigate whether occupational allergy to peach tree pollen as a member of rosaceae family is ige-mediated and to evaluate the cross-reactivity among rosaceae family pollens. allergen skin test and conjunctival challenge test were performed; enzyme linked immune-sorbent assay (elisa), inhibiting elisa, western immunoblo ...201526742437
isolation and expression analysis of four hd-zip iii family genes targeted by microrna166 in peach.microrna166 (mir166) is known to have highly conserved targets that encode proteins of the class iii homeodomain-leucine zipper (hd-zip iii) family, in a broad range of plant species. to further understand the relationship between hd-zip iii genes and mir166, four hd-zip iii family genes (pphb14, pphb15, pphb8, and pprev) were isolated from peach (prunus persica) tissue and characterized. spatio-temporal expression profiles of the genes were analyzed. genes of the peach hd-zip iii family were pr ...201526535732
transition from two to one integument in prunus species: expression pattern of inner no outer (ino), aberrant testa shape (ats) and ettin (ett).while gymnosperm ovules have one integument, in most angiosperms two integuments surround the ovules. unitegmic ovules have arisen independently several times during the evolution of angiosperms, but the ultimate genetic cause of the presence of a single integument remains elusive. we compared species of the genus prunus that have different numbers of integuments: bitegmic species, such as prunus armeniaca (apricot) and prunus persica (peach), and unitegmic species, such as prunus incisa, analyz ...201525991552
comparative analysis of predicted plastid-targeted proteomes of sequenced higher plant genomes.plastids are actively involved in numerous plant processes critical to growth, development and adaptation. they play a primary role in photosynthesis, pigment and monoterpene synthesis, gravity sensing, starch and fatty acid synthesis, as well as oil, and protein storage. we applied two complementary methods to analyze the recently published apple genome (malus × domestica) to identify putative plastid-targeted proteins, the first using targetp and the second using a custom workflow utilizing a ...201425393533
begin at the beginning: a bac-end view of the passion fruit (passiflora) genome.the passion fruit (passiflora edulis) is a tropical crop of economic importance both for juice production and consumption as fresh fruit. the juice is also used in concentrate blends that are consumed worldwide. however, very little is known about the genome of the species. therefore, improving our understanding of passion fruit genomics is essential and to some degree a pre-requisite if its genetic resources are to be used more efficiently. in this study, we have constructed a large-insert bac ...201425260959
characterization of an uncharacterized aldo-keto reductase gene from peach and its role in abiotic stress tolerance.the aldo-keto reductase (akr) superfamily is a large enzyme group of nadp-dependent oxidoreductases with numerous roles in metabolism, but many members in this superfamily remain uncharacterized. here, ppakr1, which was cloned from the rosaceous peach tree (prunus persica), was investigated as a member of the superfamily. while ppakr1 had amino acids that are important in akrs and which belonged to the akr4 group, ppakr1 did not seem to belong to any of the akr4 subgroups. ppakr1 mrna abundance ...201424837355
genome-wide analysis of the homeodomain-leucine zipper (hd-zip) gene family in peach (prunus persica).in this study, 33 homeodomain-leucine zipper (hd-zip) genes were identified in peach using the hd-zip amino acid sequences of arabidopsis thaliana as a probe. based on the phylogenetic analysis and the individual gene or protein characteristics, the hd-zip gene family in peach can be classified into 4 subfamilies, hd-zip i, ii, iii, and iv, containing 14, 7, 4, and 8 members, respectively. the most closely related peach hd-zip members within the same subfamilies shared very similar gene structur ...201424782054
isolation and characterization of a terminal flower 1 homolog from prunus serotina ehrh.flowering control is one of the several strategies for gene containment of transgenic plants. terminal flower 1 (tfl1) is known to be involved in the transcriptional repression of genes for inflorescence development. two tfl1 transcripts with different 3' utr were cloned from black cherry (prunus serotina ehrh.) using reverse transcription-polymerase chain reaction (rt-pcr) and rapid amplification of cdna ends (race). corresponding to the two tfl1 transcripts, two pstfl1 gene sequences, 1248 bp ...201323956129
ppetac1 promotes the horizontal growth of branches in peach trees and is a member of a functionally conserved gene family found in diverse plants species.trees are capable of tremendous architectural plasticity, allowing them to maximize their light exposure under highly competitive environments. one key component of tree architecture is the branch angle, yet little is known about the molecular basis for the spatial patterning of branches in trees. here, we report the identification of a candidate gene for the br mutation in prunus persica (peach) associated with vertically oriented growth of branches, referred to as 'pillar' or 'broomy'. ppa0100 ...201323663106
genome-wide analysis of the ap2/erf superfamily in peach (prunus persica).we identified 131 ap2/erf (apetala2/ethylene-responsive factor) genes in material from peach using the gene sequences of ap2/erf amino acids of arabidopsis thaliana (brassicaceae) as probes. based on the number of ap2/erf domains and individual gene characteristics, the ap2/erf superfamily gene in peach can be classified broadly into three families, erf (ethylene-responsive factor), rav (related to abi3/vp1), and ap2 (apetala2), containing 104, 5, and 21 members, respectively, along with a solo ...201223096924
identification of putative candidate genes involved in cuticle formation in prunus avium (sweet cherry) fruit.the cuticular membrane (cm) of prunus avium (sweet cherry) and other fleshy fruit is under stress. previous research indicates that the resultant strain promotes microscopic cuticular cracking. microcracks impair the function of the cm as a barrier against pathogens and uncontrolled water loss/uptake. stress and strain result from a cessation of cm deposition during early development, while the fruit surface continues to expand. the cessation of cm deposition, in turn, may be related to an early ...201222610921
histone modifications and expression of dam6 gene in peach are modulated during bud dormancy release in a cultivar-dependent manner.• bud dormancy release in many woody perennial plants responds to the seasonal accumulation of chilling stimulus. mads-box transcription factors encoded by dormancy associated mads-box (dam) genes in peach (prunus persica) are implicated in this pathway, but other regulatory factors remain to be identified. in addition, the regulation of dam gene expression is not well known at the molecular level. • a microarray hybridization approach was performed to identify genes whose expression correlates ...201221899556
the study of a spatula-like bhlh transcription factor expressed during peach (prunus persica) fruit development.extensive studies on the dry fruits of the model plant arabidopsis (arabidopsis thaliana) have revealed various gene regulators of the development and dehiscence of the siliques. peach pericarp is analogous to the valve tissues of the arabidopsis siliques. the stone (otherwise called pit) in drupes is formed through lignification of the fruit endocarp. the lignified endocarp in peach can be susceptible to split-pit formation under certain genetic as well as environmental factors. this phenomenon ...201121324706
a host rna helicase-like protein, atrh8, interacts with the potyviral genome-linked protein, vpg, associates with the virus accumulation complex, and is essential for infection.the viral genome-linked protein, vpg, of potyviruses is a multifunctional protein involved in viral genome translation and replication. previous studies have shown that both eukaryotic translation initiation factor 4e (eif4e) and eif4g or their respective isoforms from the eif4f complex, which modulates the initiation of protein translation, selectively interact with vpg and are required for potyvirus infection. here, we report the identification of two dead-box rna helicase-like proteins, ppddx ...201019880609
characterization and expression analysis of agamous-like, seedstick-like, and sepallata-like mads-box genes in peach (prunus persica) fruit.mads-box genes encode transcriptional regulators that are critical for flowering, flower organogenesis and plant development. although there are extensive reports on genes involved in flower organogenesis in model and economically important plant species, there are few reports on mads-box genes in woody plants. in this study, we have cloned and characterized agamous (ag), seedstick (stk) and sepallata (sep) homologs from peach tree (prunus persica l. batsch) and studied their expression patterns ...200919409800
characterization and expression analysis of fruitfull- and shatterproof-like genes from peach (prunus persica) and their role in split-pit formation.the fruit canning industry processes large quantities of the clingstone varieties of peach (prunus persica l. batch). the occurrence of split-pit formation--the opening of the pit and sometimes splitting of the fruit--causes deterioration of canned fruit quality. the frequency of split-pit formation is influenced by genetic and environmental factors. to increase understanding of the molecular mechanisms underlying split-pit formation in peach, we cloned and characterized the pperful and ppershp ...200717267356
comparison of peach and arabidopsis genomic sequences: fragmentary conservation of gene neighborhoods.we examined the degree of conservation of gene order in two plant species, prunus persica (peach) and arabidopsis thaliana (thale cress), whose lineages diverged more than 90 million years ago. in the three peach genomic regions studied, segments with a gene order congruent with a. thaliana were short (two to three genes in length); and for any peach region, corresponding segments were found in diverse locations in the a. thaliana genome. at the gene level and lower, the a. thaliana sequence was ...200312723043
antioxidant activity of medicinal plant polysaccharides.eleven polysaccharides have been isolated from the leaves of arctium lappa var. herkules, aloe barbadensis, althaea officinalis var. robusta, plantago lanceolata var. libor, aerial parts and roots of rudbeckia fulgida var. sullivantii, stems of mahonia aquifolium, and peach-tree (prunus persica) gum exudates. the polysaccharides were investigated for their ability to inhibit peroxidation of soyabean lecithin liposomes by oh radicals. the highest inhibition was found with glucuronoxylans of a. of ...200616797146
identification of multiple ingredients for a traditional chinese medicine preparation (bu-yang-huan-wu-tang) by liquid chromatography coupled with tandem mass spectrometry.bu-yang-huan-wu-tang (byhwt) is a popular traditional chinese medicine formula consisting of seven herbal medicines (astragalus membranaceus, angelica sinensis, paeonia lactiflora, ligusticum chuanxiong, carthamus tinctorius, amygdalus persica and pheretima aspergillum), that has been used in china for centuries to overcome stroke-induced disability. to ensure the consistency of quality, a reliable analytical method is required, therefore, we developed a liquid chromatography with tandem mass sp ...201324036516
differences in antennal sensillae of male and female peach fruit flies in relation to hosts.antennal sensillae of male and female peach fruit flies, bactrocera zonata (saunders) (diptera: tephritidae), obtained from three different host fruit species (guava, psidium guajava l. (myrtales: myrtaceae); peach, prunus persica (l.) stokes (rosales: rosaceae); and orange, citrus sinensis (l.) osbeck (sapindales: rutaceae)), were studied with scanning electron microscopy. this study was carried out to describe the different types of sensillae present on the three antennal segments (scape, pedi ...201525688086
the effects of foliar fertilization with iron sulfate in chlorotic leaves are limited to the treated area. a study with peach trees (prunus persica l. batsch) grown in the field and sugar beet (beta vulgaris l.) grown in hydroponics.crop fe deficiency is a worldwide problem. the aim of this study was to assess the effects of foliar fe applications in two species grown in different environments: peach (prunus persica l. batsch) trees grown in the field and sugar beet (beta vulgaris l. cv. "orbis") grown in hydroponics. the distal half of fe-deficient, chlorotic leaves was treated with fe sulfate by dipping and using a brush in peach trees and sugar beet plants, respectively. the re-greening of the distal (fe-treated) and bas ...201424478782
down co-regulation of light absorption, photochemistry, and carboxylation in fe-deficient plants growing in different environments.the regulation of photosynthesis through changes in light absorption, photochemistry, and carboxylation efficiency has been studied in plants grown in different environments. iron deficiency was induced in sugar beet (beta vulgaris l.) by growing plants hydroponically in controlled growth chambers in the absence of fe in the nutrient solution. pear (pyrus communis l.) and peach (prunus persica l. batsch) trees were grown in field conditions on calcareous soils, in orchards with fe deficiency-chl ...200616969716
soil transmission of beet ringspot virus to peach (prunus persica). 195713483607
utilizing immunomarking techniques to track halyomorpha halys (hemiptera: pentatomidae) movement and distribution within a peach orchard.in this study we focus on the invasive brown marmorated stink bug, halyomorpha halys (stål) (hemiptera: pentatomidae), which has a strong dispersal capacity and has had a significant impact on several cropping systems, including peach (prunus persica (l.)). management of h. halys has relied on intensive insecticide use, and thus a better understanding of its dispersal behavior may assist in developing improved management strategies. in order to investigate h. halys movement and distribution patt ...201627190711
population fluctuation and faunal indices of aphids (hemiptera, aphididae) in peach orchards in araucária, pr.aphids are sap-sucking insects that mainly attack shoots and young leaves of peach trees and many other plant species; however, knowledge of the brazilian aphid fauna is scant. the objective of this study was to identify aphid species collected in peach orchards (prunus persica batsch) and to determine their faunal indices for occurrence and dominance. the experiment was conducted from july 2005 to september 2006 in six chimarrita peach orchards in the municipality of araucária, pr, brazil. the ...200919802456
effects of simulated microgravity on male gametophyte of prunus, pyrus, and brassica species.in this study we evaluated the effect of simulated microgravity on pollen germination of both herbaceous and woody species in order to investigate the possibility of applying gametophytic selection for plant growth in the space environment. the behaviour of gametophytes exposed to the stress of clinostat rotation could be used to screen the degree of tolerance of the sporophyte to simulated microgravity. the use of male gametophyte selection overcomes the problems generally encountered by sporop ...200616937064
laboratory evaluation of differential attraction of culex pipiens pallens to fruit-based sugar baits.mosquito adults usually need to obtain sugar from floral nectaries and damaged fruits/seed pods to replenish their energy reserves. the newly developed attractive toxic sugar baits have been successfully applied in controlling various mosquito species outdoors. however, the attraction of culex pipiens pallens to different fruit-based sugar baits remains unknown. in the present study, we selected nine common fruit species, prepared the fruit-based sugar solutions, and investigated the attractiven ...201627456936
traditional knowledge on poisonous plants of udhampur district of jammu and kashmir, india.poisonous plants comprise the third largest category of poisons known around the world. other than affecting the humans directly, they are the major cause of economic losses in the livestock industry since the advent of civilisation. aim of the present study was to collect and systematically document the traditional knowledge of poisonous plants of udhampur district for the benefit of humanity before it is entombed forever.201424412380
[comparative study on eight trace elements in twelve flower medicines].eight trace elements such as ca, cu, fe, mn, zn, k, mg and na in twelve kinds of flower medicines were determined by flame-atomic absorption spectrometry with air-acetylene flame. the flower medicines include pueraria lobata ohwi., gomphrena globosa l., chrysanthemum morifolium ramat., prunus persica (l.) batsch., canna indica l., pyrus bretschneideri rehd p. spp, rosa chinensis jacq., celosia cristata l., sophora japonica l., saussurea medusa maxim. , iris lactea var. chinensis (fisch.) koidz. ...200919798991
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