Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| identification of novel splice variants of the arabidopsis dcl2 gene. | in arabidopsis thaliana, dicer-like protein 2 (dcl2) cleaves double-stranded virus rna, playing an essential role in the rna interference pathway. here, we describe three alternative splicing (as) forms of atdcl2: in one, both intron 8 and intron 10 are retained in the mrna, in second only intron 8 is retained and in the third no intron is retained, but there is a deletion of 56 nucleotides at the end of exon 10. these splicing forms are present in stems and leaves at different development stage ... | 2009 | 19011859 |
| quantifying the introgressive hybridisation propensity between transgenic oilseed rape and its wild/weedy relatives. | in order to estimate the introgressive hybridisation propensity (ihp) between genetically modified (gm) oilseed rape (brassica napus) and certain of its cross-compatible wild/weedy relatives at the landscape level, a conceptual approach was developed. a gene flow index was established enclosing the successive steps to successfully achieve introgressive hybridisation: wild/weedy relatives and oilseed rape should co-occur, have overlapping flowering periods, be compatible, produce viable and ferti ... | 2009 | 18253849 |
| cell wall invertase 4 is required for nectar production in arabidopsis. | to date, no genes have been reported to directly affect the de novo production of floral nectar. in an effort to identify genes involved in nectar production, the affymetrix((r)) ath1 genechip was previously used to examine global gene expression profiles in arabidopsis thaliana nectaries. one of the genes displaying highly enriched expression in nectaries was cell wall invertase 4 (atcwinv4, at2g36190), which encodes an enzyme that putatively catalyses the hydrolysis of sucrose into glucose and ... | 2009 | 19861655 |
| brassica orthologs from banyuls belong to a small multigene family, which is involved in procyanidin accumulation in the seed. | as part of a research programme focused on flavonoid biosynthesis in the seed coat of brassica napus l. (oilseed rape), orthologs of the banyuls gene that encoded anthocyanidin reductase were cloned in b. napus as well as in the related species brassica rapa and brassica oleracea. b. napus genome contained four functional copies of ban, two originating from each diploid progenitor. amino acid sequences were highly conserved between the brassicaceae including b. napus, b. rapa, b. oleracea as wel ... | 2009 | 19760260 |
| overexpression of a brassica rapa ngatha gene in arabidopsis thaliana negatively affects cell proliferation during lateral organ and root growth. | in an effort to elucidate biological functions of transcription factors of brassica rapa l. (ssp. pekinensis), an ngatha homolog, brnga1, that belongs to the b3-type transcription factor superfamily was identified and expressed in arabidopsis thaliana under the control of the cauliflower mosaic virus (camv) 35s promoter. arabidopsis plants overexpressing brnga1, named brnga1ox, displayed markedly reduced organ growth compared with the wild type: lateral organs, such as leaves, flowers and cotyle ... | 2009 | 19880400 |
| spontaneous capture of oilseed rape (brassica napus) chloroplasts by wild b. rapa: implications for the use of chloroplast transformation for biocontainment. | environmental concerns over the cultivation of genetically modified (gm) crops largely centre on the ecological consequences following gene flow to wild relatives. one attractive solution is to deploy biocontainment measures that prevent hybridization. chloroplast transformation is the most advanced biocontainment method but is compromised by chloroplast capture (hybridization through the maternal lineage). to date, however, there is a paucity of information on the frequency of chloroplast captu ... | 2009 | 19198841 |
| sewage sludge applied to agricultural soil: ecotoxicological effects on representative soil organisms. | application of sewage sludge to agricultural lands is a current practice in eu. european legislation permits its use when concentrations of metals in soil do not increase above the maximum permissible limits. in order to assess the fate and the effects on representative soil organisms of sewage sludge amendments on agricultural lands, a soil microcosm (multi-species soil system-ms3) experiment was performed. the ms3 columns were filled with spiked soil at three different doses: 30, 60 and 120tha ... | 2009 | 19261330 |
| diversity, distribution and role of wild crucifers in major cabbage and kale growing areas of kenya. | an investigation of the diversity and distribution of wild crucifer species and their importance for cultivated crucifers was conducted during 2005 and 2006 in the highland and mid-altitude semi-arid areas of kenya. thirteen species of wild crucifers in nine genera were recorded: raphanus raphanistrum, erucastrum arabicum, sisymbrium officinale, crambe kilimandscharica, capsella bursa-pastoris, rorippa nudiuscula, ro. micrantha, ro. microphylla, lepidium bonariense, coronopus didymus, brassica r ... | 2009 | 19063754 |
| metabolic changes in agrobacterium tumefaciens-infected brassica rapa. | agrobacterium has the ability to transfer its genetic material, t-dna, into the plant genome. the unique interaction between the bacterium and its host plant has been well studied at the transcriptome, but not at the metabolic level. for a better understanding of this interaction it is necessary to investigate the metabolic changes of the host plant upon infection with agrobacterium tumefaciens. this study investigated the metabolic response of brassica rapa to infection with disarmed and tumor- ... | 2009 | 19346030 |
| plant fitness assessment for wild relatives of insect resistant crops. | risk assessments of new insect-resistant crops will need to estimate the potential for increased weediness of wild crop relatives as a consequence of gene flow. when field experiments are precluded by containment concerns, simulation experiments can identify hazards or measure expected differences between gmos and parental plants. to measure plant fitness consequences of wild plant protection from bt-susceptible herbivores, we used topical sprays of bacterial bacillus thuringiensis larvacide (bt ... | 2009 | 19419653 |
| a novel plant cysteine-rich peptide family conferring cadmium tolerance to yeast and plants. | we have identified a novel cdna clone, termed dccdt1, from digitaria ciliaris, that confers cadmium (cd)-tolerance to yeast (saccharomyces cerevisiae). the gene encodes a predicted peptide of 55 amino acid residues of which 15 (27.3%) are cysteine residues. we found that monocotyledonous plants possess multiple dccdt1 homologues, for example rice contains five dccdt1 homologues (designated oscdt1~5), whereas dicotyledonous plants, including arabidopsis thaliana, brassica rapa, poplar (populus tr ... | 2009 | 19816106 |
| genome-wide identification of glucosinolate synthesis genes in brassica rapa. | glucosinolates play important roles in plant defense against herbivores and microbes, as well as in human nutrition. some glucosinolate-derived isothiocyanate and nitrile compounds have been clinically proven for their anticarcinogenic activity. to better understand glucosinolate biosynthesis in brassica rapa, we conducted a comparative genomics study with arabidopsis thaliana and identified total 56 putative biosynthetic and regulator genes. this established a high colinearity in the glucosinol ... | 2009 | 19456863 |
| interaction between plants and bacteria: glucosinolates and phyllospheric colonization of cruciferous vegetables by enterobacter radicincitans dsm 16656. | for determining interactive plant-bacterial effects between glucosinolates and phyllospheric colonization by a plant growth-promoting strain, enterobacter radicincitans dsm 16656, in cruciferous vegetables, the extent of bacterial colonization was assessed in 5 cruciferous vegetables (brassica juncea, brassica campestris, brassica oleracea var. capitata, brassica rapa var. alboglabra, nasturtium officinale) using a species-specific taqman probe and quantitative real-time pcr. colonization abilit ... | 2009 | 19556746 |
| enhancement of tolerance to soft rot disease in the transgenic chinese cabbage (brassica rapa l. ssp. pekinensis) inbred line, kenshin. | we developed a transgenic chinese cabbage (brassica rapa l. ssp. pekinensis) inbred line, kenshin, with high tolerance to soft rot disease. tolerance was conferred by expression of n-acyl-homoserine lactonase (ahl-lactonase) in chinese cabbage through an efficient agrobacterium-mediated transformation method. to synthesize and express the ahl-lactonase in chinese cabbage, the plant was transformed with the aii gene (ahl-lactonase gene from bacillus sp. gh02) fused to the pinii signal peptide (pr ... | 2009 | 19680657 |
| simultaneous identification of glucosinolates and phenolic compounds in a representative collection of vegetable brassica rapa. | brassica raparapa group is widely distributed and consumed in northwestern spain. the consumption of brassica vegetables has been related to human health due to their phytochemicals, such as glucosinolates and phenolic compounds that induce a variety of physiological functions including antioxidant activity, enzymes regulation and apoptosis control and the cell cycle. for first time in brassica crops, intact glucosinolates and phenolic compounds were simultaneously identified and characterized. ... | 2009 | 19683241 |
| genetic and physiological determinants of streptomyces scabies pathogenicity. | summary common scab is a severe disease worldwide affecting tap root crops and potato tubers. it is caused by soil-borne filamentous bacteria belonging to the genus streptomyces. streptomycetes usually are saprophytic microorganisms, but a few species have acquired the ability to infect underground plant tissues. the predominant causal agent of potato scab worldwide is streptomyces scabies. the production of phytotoxins called thaxtomins is essential for the virulence of common scab-causing agen ... | 2009 | 19694949 |
| genome-wide identification of nbs-encoding resistance genes in brassica rapa. | nucleotide-binding site (nbs)-encoding resistance genes are key plant disease-resistance genes and are abundant in plant genomes, comprising up to 2% of all genes. the availability of genome sequences from several plant models enables the identification and cloning of nbs-encoding genes from closely related species based on a comparative genomics approach. in this study, we used the genome sequence of brassica rapa to identify nbs-encoding genes in the brassica genome. we identified 92 non-redun ... | 2009 | 19838736 |
| jasmonate-dependent plant defense restricts thrips performance and preference. | the western flower thrips (frankliniella occidentalis [pergande]) is one of the most important insect herbivores of cultivated plants. however, no pesticide provides complete control of this species, and insecticide resistance has emerged around the world. we previously reported the important role of jasmonate (ja) in the plant's immediate response to thrips feeding by using an arabidopsis leaf disc system. in this study, as the first step toward practical use of ja in thrips control, we analyze ... | 2009 | 19635132 |
| impact of cry1ac-carrying brassica rapa subsp. pekinensis on leaf bacterial community. | the effects of chinese cabbage (brassica rapa subsp. pekinensis) carrying cry1ac derived from bacillus thuringiensis (bt) on leaf bacterial community were examined by analyzing the horizontal transfer of trans-gene fragments from plants to bacteria. the effect of plant pathogenic bacteria on the gene transfer was also examined using pseudomonas syringae pathovar. maculicola. the frequency of hygromycin-resistant bacteria did not alter in bt leaves, though slight increase was observed in pseudomo ... | 2009 | 19229489 |
| auxin amidohydrolases from brassica rapa cleave the alanine conjugate of indolepropionic acid as a preferable substrate: a biochemical and modeling approach. | two auxin amidohydrolases, briar3 and brill2, from chinese cabbage [brassica rapa l. ssp. pekinensis (lour.) hanelt] were produced by heterologous expression in escherichia coli, purified, and screened for activity towards n-(indol-3-ylacetyl)-l-alanine (iaa-ala) and the long-chain auxin-amino acid conjugates, n-[3-(indol-3-yl)propionyl]-l-alanine (ipa-ala) and n-[4-(indol-3-yl)butyryl]-l-alanine (iba-ala). ipa-ala was shown to be the favored substrate of both enzymes, but brill2 was approximate ... | 2009 | 19602499 |
| expression of a glutathione reductase from brassica rapa subsp. pekinensis enhanced cellular redox homeostasis by modulating antioxidant proteins in escherichia coli. | glutathione reductase (gr) is an enzyme that recycles a key cellular antioxidant molecule glutathione (gsh) from its oxidized form (gssg) thus maintaining cellular redox homeostasis. a recombinant plasmid to overexpress a gr of brassica rapa subsp. pekinensis (brgr) in e. coli bl21 (de3) was constructed using an expression vector pkm260. expression of the introduced gene was confirmed by semiquantitative rt-pcr, immunoblotting and enzyme assays. purification of the brgr protein was performed by ... | 2009 | 19936628 |
| isolation and characterization of ngrlk1, a receptor-like kinase of nicotiana glutinosa that interacts with the elicitin of phytophthora capsici. | elicitins, extracellular proteins from phytophthora fungi, elicit a hypersensitivity response (hr), including systemic acquired resistance, in some plants. the elicitin capsicein (approximately 10 kda) was purified by fplc from culture filtrates of p. capsici. purified native and recombinant capsicein induced a hypersensitive response in leaves of the non-host plants nicotiana glutinosa and brassica rapa subsp. pekinensis. to search for candidate capsicein-interacting proteins from n. glutinosa, ... | 2010 | 19449126 |
| flight activity and dispersal of the cabbage seedpod weevil (coleoptera: curculionidae) are related to atmospheric conditions. | the cabbage seedpod weevil, ceutorhynchus obstrictus (marsham) (coleoptera: curculionidae), is an invasive pest of canola (brassica napus l. and brassica rapa l.) in western canada. under current climatic conditions, c. obstrictus is spreading from established populations in southwestern alberta at ˜55 km/yr. we studied the influence of climatic conditions on c. obstrictus flight behavior in 2007 and 2008 and eastward dispersal from the western border of saskatchewan from 2002 to 2007. positive ... | 2010 | 22127159 |
| three homologous genes encoding sn-glycerol-3-phosphate acyltransferase 4 exhibit different expression patterns and functional divergence in brassica napus. | brassica napus is an allotetraploid (aacc) formed from the fusion of two diploid progenitors, brassica rapa (aa) and brassica oleracea (cc). polyploidy and genome-wide rearrangement during the evolution process have resulted in genes that are present as multiple homologs in the b. napus genome. in this study, three b. napus homologous genes encoding endoplasmic reticulum-bound sn-glycerol-3-phosphate acyltransferase 4 (gpat4) were identified and characterized. although the three gpat4 homologs s ... | 2010 | 21173024 |
| assessment of genetic diversity of accessions in brassicaceae genetic resources by frequency distribution analysis of s haplotypes. | plant genetic resources are important sources of genetic variation for improving crop varieties as breeding materials. conservation of such resources of allogamous species requires maintenance of the genetic diversity within each accession to avoid inbreeding depression and loss of rare alleles. for assessment of genetic diversity in the self-incompatibility locus (s locus), which is critically involved in the chance of mating, we developed a dot-blot genotyping method for self-incompatibility ( ... | 2010 | 20039015 |
| screening of dried plant seed extracts for adiponectin production activity and tumor necrosis factor-alpha inhibitory activity on 3t3-l1 adipocytes. | to search for dried plant seeds with potent anti-diabetes activity, we conducted a large scale screening for inhibitory activity on tumor necrosis factor-alpha and facilitating activity on adiponectin production in vitro. these activities in 3t3-l1 adipocytes were screened from ethanol extracts of 20 kinds of dried plant seed marketed in japan. komatsuna (brassica rapa var. perviridis), common bean (phaseolus vulgaris l.), qing geng cai (brassica rapa var. chinensis), green soybean (glycine max) ... | 2010 | 20717728 |
| suppressive effect of yamato-mana (brassica rapa l. oleifera group) constituent 3-butenyl glucosinolate (gluconapin) on postprandial hypertriglyceridemia in mice. | we examined the bioactivity of yamato-mana (brassica rapa l. oleifera group) constituent glucosinolates and found that 3-butenyl glucosinolate (gluconapin) decreased the plasma triglyceride gain induced by corn oil administration to mice. however, phenethyl glucosinolate (gluconasturtiin) had little effect. 2-propenyl glucosinolate (sinigrin) also reduced the plasma triglyceride level, which suggests that alkenyl glucosinolates might be promising agents to prevent postprandial hypertriglyceridem ... | 2010 | 20530888 |
| an insertional mutagenesis system for analyzing the chinese cabbage genome using agrobacterium t-dna. | in this study, we applied insertional mutagenesis using agrobacterium transfer dna to functionally characterize the gene of brassica rapa l. ssp. pekinensis. the specific objectives were to: (i) develop and apply a gene tagging system using plasmid rescue and inverse pcr, (ii) select and analyze mutant lines, and (iii) analyze the phenotypic characteristics of mutants. a total of 3,400 insertional mutant lines were obtained from the chinese cabbage cultivar, 'seoul', using optimized condition. p ... | 2010 | 20195907 |
| a comparative study of human health risks via consumption of food crops grown on wastewater irrigated soil (peshawar) and relatively clean water irrigated soil (lower dir). | food crops irrigated with wastewater are mostly contaminated with heavy metals and considered as a main pathway for human exposure. in this study, soil and food crops samples were collected from wastewater irrigated soils, background and relatively less polluted areas. results of the sequential extraction and total metals concentrations in soils indicated that wastewater irrigation has significantly increased (p > or = 0.001) the bioavailable and total metal contents in wastewater irrigated soil ... | 2010 | 20399016 |
| chalcone glycosides isolated from aerial parts of brassica rapa l. 'hidabeni' suppress antigen-stimulated degranulation in rat basophilic leukemia rbl-2h3 cells. | we isolated three chalcone glycosides along with other glycoside constituents from the aerial parts of brassica rapa l. 'hidabeni' and examined the effects of these compounds on the antigen-stimulated degranulation in rat basophilic leukemia rbl-2h3 cells. treatments with both 4'-o-β-d-glucopyranosyl-4-hydroxy-3'-methoxychalcone (c1) and 4'-o-β-d-glucopyranosyl-3',4-dimethoxychalcone (c2) markedly inhibited antigen (ag)-stimulated degranulation. to gain further insight into the inhibitory mechan ... | 2010 | 20801664 |
| evolutionary trajectory of turnip mosaic virus populations adapting to a new host. | little is known about how some plant viruses establish successful cross-species transmission whilst others do not; the genetic basis for adaptation is largely unknown. this study investigated the genetic changes that occurred using the progeny of an infectious clone, p35tunos, derived from the turnip mosaic virus (tumv) uk 1 isolate, which has a brassica host type, but rarely infects raphanus systemically and then only asymptomatically. the genetic trajectory leading to viral adaptation was stud ... | 2010 | 19906944 |
| qtl mapping of clubroot resistance in radish (raphanus sativus l.). | a qtl analysis for clubroot resistance (cr) of radish was performed using an f(2) population derived from a crossing of a cr japanese radish and a clubroot-susceptible (cs) chinese radish. f(3) plants obtained by selfing of f(2) plants were used for the cr tests. the potted seedlings were inoculated and the symptom was evaluated 6 weeks thereafter. the mean disease indexes of the f(3) plants were used for the phenotype of the f(2). the results of two cr tests were analyzed for the presence of qt ... | 2010 | 20012934 |
| genetic relationships among turnip (brassica rapa var. rapa) genotypes. | turnip (brassica rapa var. rapa) is one of the main vegetables consumed by people living in eastern anatolia in turkey. in this region, farmers obtain their own seeds for production, which results in considerable morphological variability. we examined the genetic variation and relationships among 11 turnip genotypes sampled from diverse environments of the erzurum region located in eastern anatolia in turkey. thirty-two operon rapd primers were screened; among them, 20 gave reproducible and clea ... | 2010 | 20506086 |
| overexpression of brmorn, a novel 'membrane occupation and recognition nexus' motif protein gene from chinese cabbage, promotes vegetative growth and seed production in arabidopsis. | proteins that contain membrane occupation and recognition nexus (morn) motifs regulate various aspects of cellular metabolism by localizing proteins in different cellular organelles. the full-length brassica rapa morn motif protein (brmorn) cdna consists of 1,510 bp encoding 502 deduced amino acids with a predicted molecular mass of 55.8 kda and an isoelectric point of 9.72. brmorn is a novel protein composed of two n-terminal transmembrane helices and seven c-terminal morn motifs and it appears ... | 2010 | 20016940 |
| novel self-compatible lines of brassica rapa l. isolated from the japanese bulk-populations. | self-incompatibility (si) in brassicaceae is sporophytically controlled by a single s-locus with multi allelic variety. the male s determinant, sp11/scr (s-locus protein 11/s-locus cysteine-rich protein), is a small cysteine-rich protein, and the female s determinant, srk (s-locus receptor kinase), functions as a receptor for sp11 at the surface of stigma papilla cells. although a few of the following downstream factors in the sp11-srk signaling cascade have been identified, a comprehensive unde ... | 2010 | 20558895 |
| the rapidly evolving centromere-specific histone has stringent functional requirements in arabidopsis thaliana. | centromeres control chromosome inheritance in eukaryotes, yet their dna structure and primary sequence are hypervariable. most animals and plants have megabases of tandem repeats at their centromeres, unlike yeast with unique centromere sequences. centromere function requires the centromere-specific histone cenh3 (cenp-a in human), which replaces histone h3 in centromeric nucleosomes. cenh3 evolves rapidly, particularly in its n-terminal tail domain. a portion of the cenh3 histone-fold domain, t ... | 2010 | 20628040 |
| turnip mosaic virus (tumv) is able to use alleles of both eif4e and eif(iso)4e from multiple loci of the diploid brassica rapa. | three copies of eif4e and three copies of eif(iso)4e have been identified and sequenced from a turnip mosaic virus (tumv)-susceptible, inbred, diploid brassica rapa line, r-o-18. one of the copies of eif4e lacked exons 2 and 3 and appeared to be a pseudogene. the two other copies of eif4e and two of the three copies of eif(iso)4e were isolated from a bacterial artificial chromosome library of r-o-18. using an arabidopsis line (col-0::dspm) with a transposon knock-out of the eif(iso)4e gene which ... | 2010 | 20672877 |
| lactococcus fujiensis sp nov., a lactic acid bacterium isolated from vegetable. | three strains of lactic acid bacteria isolated from vegetable of chinese cabbage (brassica rapa l. var. glabra regel) were characterized taxonomically. they were gram-positive, catalase-negative, facultatively anaerobic cocci that did not produce gas from glucose and formed l-lactic acid. their major fatty acids were c18:1omega9cis, c16:0, c14:0 and summed feature 10. morphological, physiological and phylogenetic data indicated that the strains belong to the genus lactococcus. these strains shar ... | 2010 | 20675438 |
| genomics of biotrophic, plant-infecting plasmodiophorids using in vitro dual cultures. | the plasmodiophorids are a phylogenetically distinct group of parasitic protists that infect plants and stramenopiles, causing several important agricultural diseases. because of the obligate intracellular part of their lifecycle, none of the plasmodiophorids has been axenically cultured. further, the molecular biology of the plasmodiophorids is poorly understood because pure cultures are not available from any species. we report on an in-vitro dual culture system of the plasmodiophorids plasmod ... | 2010 | 21183405 |
| uptake of human pharmaceuticals by plants grown under hydroponic conditions. | cabbage (brassica rapa var. pekinensis) and wisconsin fast plants (brassica rapa) were chosen for a proof of concept study to determine the potential uptake and accumulation of human pharmaceuticals by plants. these plants were grown hydroponically under high-pressure sodium lamps in one of two groups including a control and test group exposed to pharmaceuticals. the control plants were irrigated with a recirculating hoagland's nutrient solution while the test plants were irrigated with a hoagla ... | 2010 | 20096438 |
| s-glycoprotein-like protein regulates defense responses in nicotiana plants against ralstonia solanacearum. | rsrga4 (for ralstonia solanacearum-responsive gene a4) encodes a polypeptide similar to s-locus glycoprotein (sgp) from brassica rapa and sgp-like proteins from ipomoea trifida and medicago truncatula. therefore, we designated rsrga4 as ntsglp (for nicotiana tabacum sgp-like protein) and nbsglp (its nicotiana benthamiana ortholog). nbsglp is expressed in root, leaf, petal, gynoecium, and stamen. expression of nbsglp was strongly induced by inoculation with an avirulent strain of r. solanacearum ... | 2010 | 20118275 |
| addition of restriction fragment length polymorphism markers to the genetic linkage map of brassica rapa l. (syn. campestris). | genetic linkage analysis of 151 restriction fragment length polymorphism (rflp) loci, that included eight new loci, detected by the six probes in the present study, and four trait loci including seed colour, leaf pubescence, resistance to white rust caused by albugo candida race-2 (ac-2) and race-7 (ac-7) employing the mapmaker/exp 3.0 programme led to the development of 10 linkage groups (lgs) spanning over 44.4 centimorgan (cm) to 130.4 cm containing 9 to 22 loci and two short lgs with two or ... | 2010 | 20158162 |
| a novel detection system for the genetically modified canola (brassica rapa) line rt73. | the herbicide-tolerant genetically modified roundup ready canola (brassica napus) line rt73 has been approved worldwide for use in animal feed and human food. however, rt73 brassica rapa lines derived from interspecific crosses with rt73 b. napus have not been approved in japan. here, we report on a novel system using individual kernel analyses for the qualitative detection of rt73 b. rapa in canola grain samples. we developed a duplex real-time polymerase chain reaction (pcr) method to discrimi ... | 2010 | 21049930 |
| sequence and structure of brassica rapa chromosome a3. | the species brassica rapa includes important vegetable and oil crops. it also serves as an excellent model system to study polyploidy-related genome evolution because of its paleohexaploid ancestry and its close evolutionary relationships with arabidopsis thaliana and other brassica species with larger genomes. therefore, its genome sequence will be used to accelerate both basic research on genome evolution and applied research across the cultivated brassica species. | 2010 | 20875114 |
| a brassica exon array for whole-transcript gene expression profiling. | affymetrix genechip® arrays are used widely to study transcriptional changes in response to developmental and environmental stimuli. genechip® arrays comprise multiple 25-mer oligonucleotide probes per gene and retain certain advantages over direct sequencing. for plants, there are several public genechip® arrays whose probes are localised primarily in 3' exons. plant whole-transcript (wt) genechip® arrays are not yet publicly available, although wt resolution is needed to study complex crop gen ... | 2010 | 20862292 |
| genetic analysis of interspecific incompatibility in brassica rapa. | in interspecific pollination of brassica rapa stigmas with brassica oleracea pollen grains, pollen tubes cannot penetrate stigma tissues. this trait, called interspecific incompatibility, is similar to self-incompatibility in pollen tube behaviors of rejected pollen grains. since some b. rapa lines have no interspecific incompatibility, genetic analysis of interspecific incompatibility was performed using two f(2) populations. analysis with an f(2) population between an interspecific-incompatibl ... | 2010 | 20414635 |
| robust circadian rhythms of gene expression in brassica rapa tissue culture. | circadian clocks provide temporal coordination by synchronizing internal biological processes with daily environmental cycles. to date, study of the plant circadian clock has emphasized arabidopsis (arabidopsis thaliana) as a model, but it is important to determine the extent to which this model applies in other species. accordingly, we have investigated circadian clock function in brassica rapa. in arabidopsis, analysis of gene expression in transgenic plants in which luciferase activity is exp ... | 2010 | 20406912 |
| a rich tilling resource for studying gene function in brassica rapa. | the brassicaceae family includes the model plant arabidopsis thaliana as well as a number of agronomically important species such as oilseed crops (in particular brassica napus, b. juncea and b. rapa) and vegetables (eg. b. rapa and b. oleracea). separated by only 10-20 million years, brassica species and arabidopsis thaliana are closely related, and it is expected that knowledge obtained relating to arabidopsis growth and development can be translated into brassicas for crop improvement. moreov ... | 2010 | 20380715 |
| identification of differential gene expression in brassica rapa nectaries through expressed sequence tag analysis. | nectaries are the floral organs responsible for the synthesis and secretion of nectar. despite their central roles in pollination biology, very little is understood about the molecular mechanisms underlying nectar production. this project was undertaken to identify genes potentially involved in mediating nectary form and function in brassica rapa. | 2010 | 20098697 |
| cross-species approaches to seed dormancy and germination: conservation and biodiversity of aba-regulated mechanisms and the brassicaceae dog1 genes. | seed dormancy is genetically determined with substantial environmental influence mediated, at least in part, by the plant hormone abscisic acid (aba). the aba-related transcription factor abi3/vp1 (aba insensitive3/viviparous1) is widespread among green plants. alternative splicing of its transcripts appears to be involved in regulating seed dormancy, but the role of abi3/vp1 goes beyond seeds and dormancy. in contrast, dog1 (delay of germination 1), a major quantitative trait gene more specific ... | 2010 | 20013031 |
| sequence-level comparative analysis of the brassica napus genome around two stearoyl-acp desaturase loci. | we conducted a sequence-level comparative analyses, at the scale of complete bacterial artificial chromosome (bac) clones, between the genome of the most economically important brassica species, brassica napus (oilseed rape), and those of brassica rapa, the genome of which is currently being sequenced, and arabidopsis thaliana. we constructed a new b. napus bac library and identified and sequenced clones that contain homoeologous regions of the genome including stearoyl-acp desaturase-encoding g ... | 2010 | 19929877 |
| analysis of brassica rapa ests: gene discovery and expression patterns of ap2/erf family genes. | chinese cabbage (brassica rapa subsp. pekinensis) is among the most important vegetables and is widely cultivated in world. genes in the ap2/erf family encode transcriptional regulators that serve a variety of functions in the plants. expressed sequence tags (ests) are created by partially sequencing randomly isolated gene transcripts and have proved valuable in molecular biology. starting from the database with 142 947 ests of b. rapa, 62 putative ap2/erf family genes were identified by in sili ... | 2010 | 19701799 |
| construction of an integrated genetic linkage map for the a genome of brassica napus using ssr markers derived from sequenced bacs in b. rapa. | the multinational brassica rapa genome sequencing project (brgsp) has developed valuable genomic resources, including bac libraries, bac-end sequences, genetic and physical maps, and seed bac sequences for brassica rapa. an integrated linkage map between the amphidiploid b. napus and diploid b. rapa will facilitate the rapid transfer of these valuable resources from b. rapa to b. napus (oilseed rape, canola). | 2010 | 20969760 |
| regional association analysis delineates a sequenced chromosome region influencing antinutritive seed meal compounds in oilseed rape. | this study describes the use of regional association analyses to delineate a sequenced region of a brassica napus chromosome with a significant effect on antinutritive seed meal compounds in oilseed rape. a major quantitative trait locus (qtl) influencing seed colour, fibre content, and phenolic compounds was mapped to the same position on b. napus chromosome a9 in biparental mapping populations from two different yellow-seeded × black-seeded b. napus crosses. sequences of markers spanning the q ... | 2010 | 21076507 |
| regulation of tissue-specific expression of spatula, a bhlh gene involved in carpel development, seedling germination, and lateral organ growth in arabidopsis. | spatula is a bhlh transcription factor that promotes growth of tissues arising from the carpel margins, including the septum and transmitting tract. it is also involved in repressing germination of newly harvested seeds, and in inhibiting cotyledon, leaf, and petal expansion. using a reporter gene construct, its expression profile was fully defined. consistent with its known functions, spt was expressed in developing carpel margin tissues, and in the hypocotyls and cotyledons of germinating seed ... | 2010 | 20176890 |
| identification of qtls that control clubroot resistance in brassica oleracea and comparative analysis of clubroot resistance genes between b. rapa and b. oleracea. | to perform comparative studies of cr (clubroot resistance) loci in brassica oleracea and brassica rapa and to develop marker-assisted selection in b. oleracea, we constructed a b. oleracea map, including specific markers linked to cr genes of b. rapa. we also analyzed cr-qtls using the mean phenotypes of f(3) progenies from the cross of a resistant double-haploid line (anju) with a susceptible double-haploid line (gc). in the nine linkage groups obtained (o1-o9), the major qtl, pb-bo(anju)1, was ... | 2010 | 20069415 |
| overexpression of the ethylene-responsive factor gene brerf4 from brassica rapa increases tolerance to salt and drought in arabidopsis plants. | ethylene-responsive factors (erfs), within a subgroup of the ap2/erf transcription factor family, are involved in diverse plant reactions to biotic or abiotic stresses. here, we report that overexpression of an erf gene from brassica rapa ssp. pekinensis (brerf4) led to improved tolerance to salt and drought stresses in arabidopsis. it also significantly affected the growth and development of transgenic plants. we detected that salt-induced expressions of a transcriptional repressor gene, aterf4 ... | 2010 | 20803085 |
| predicted no effect concentration (pnec) for triclosan to terrestrial species (invertebrates and plants). | triclosan (tcs) is an antimicrobial agent which is used as a broad-spectrum bacteriostatic and found in personal care products, and due to this it is spread in the environment. aquatic toxicity studies have been reported, but little data is available for its toxicity to terrestrial organisms. the main purpose of this study was to assess the toxicity of tcs in the terrestrial environment, using a battery of soil species, belonging to different taxonomic levels. the species included the invertebra ... | 2010 | 20227762 |
| development of a high density integrated reference genetic linkage map for the multinational brassica rapa genome sequencing project. | we constructed a high-density brassica rapa integrated linkage map by combining a reference genetic map of 78 doubled haploid lines derived from chiifu-401-42 × kenshin (ckdh) and a new map of 190 f2 lines derived from chiifu-401-42 × rapid cycling b. rapa (crf2). the integrated map contains 1017 markers and covers 1262.0 cm of the b. rapa genome, with an average interlocus distance of 1.24 cm. high similarity of marker order and position was observed among the linkage groups of the maps with fe ... | 2010 | 21076509 |
| design of a brassica rapa core collection for association mapping studies. | a brassica rapa collection of 239 accessions, based on two core collections representing different morphotypes from different geographical origins, is presented and its use for association mapping is illustrated for flowering time. we analyzed phenotypic variation of leaf and seed pod traits, plant architecture, and flowering time using data collected from three field experiments and evaluated the genetic diversity with a set of ssr markers. the wageningen university and research centre (wur) an ... | 2010 | 21076504 |
| response of glucosinolate and flavonoid contents and composition of brassica rapa ssp. chinensis (l.) hanelt to silica formulations used as insecticides. | silica-based substances have increased in popularity in recent years as alternative insecticides in horticultural crop protection. however, no research has been conducted into the influence of silica on plant biochemistry. formulations (fossil shield 90.0s, ae r974, al-06-109, surround) were applied electrostatically on pak choi. plants were harvested on two dates to measure immediate (first) and decelerated (second) influence, as well as recovery following the removal of silica formulations. th ... | 2010 | 21073193 |
| brflc2 (flowering locus c) as a candidate gene for a vernalization response qtl in brassica rapa. | flowering time is an important agronomic trait, and wide variation exists among brassica rapa. in arabidopsis, flowering locus c (flc) plays an important role in modulating flowering time and the response to vernalization. brassica rapa contains several paralogues of flc at syntenic regions. brflc2 maps under a major flowering time and vernalization response quantitative trait locus (qtl) at the top of a02. here the effects of vernalization on flowering time in a double haploid (dh) population a ... | 2010 | 20231331 |
| composition analysis and anti-hypoxia activity of polysaccharide from brassica rapa l. | crude water-soluble polysaccharides (brp) were extracted from the root of brassica rapa l. using boiling-water. the polysaccharides were successively purified by chromatography on deae-cellulose and sephadex g-100 column, giving three major polysaccharide fractions termed brp1-1, brp2-1, brp3-1. the gel permeation chromatography (gpc) analysis showed that the average molecular weight (mw) of polysaccharides (brp1-1, brp2-1, brp3-1) were approximately 5.53×10(3) da, 3.35×10(4) da and 3.37×10(4) d ... | 2010 | 20678519 |
| integration of genetic, physical, and cytogenetic maps for brassica rapa chromosome a7. | bacterial artificial chromosome (bac) contigs have been genetically mapped to the 10 linkage groups of brassica rapa by bac end sequences (bes). to integrate the genetic, physical, and cytogenetic maps, fluorescence in situ hybridization (fish) was used to anchor the assembly of bac contigs onto brassica chromosomes using representative bacs. this bac-fish approach can be used to identify chromosome arms on separate mitotic metaphase chromosomes or to map multiple bacs to single long pachytene c ... | 2010 | 20628251 |
| mapping of quantitative trait loci for high level of self-incompatibility in brassica rapa l. | the level of self-incompatibility (si) is important to the purity of f1 seeds produced using the si system of brassica vegetables. to analyze the genetic basis of the level of si, we generated an f2 population derived from a cross between a turnip inbred line showing a high level of si and a chinese cabbage inbred line showing a low level, and evaluated the level of si under insect pollination in two years. we constructed a detailed linkage map of brassica rapa from the f2 progeny, consisting of ... | 2010 | 20616857 |
| effect of organic production and fertilizer variables on the sensory properties of pac choi (brassica rapa var. mei qing choi) and tomato (solanum lycopersicum var. bush celebrity). | the increased popularity of organic production has amplified the need for research that will help in understanding how this production system affects the final quality of vegetables. the effects of organic and conventional production on the sensory characteristics of pac choi (often called bok choy) and tomato were studied. samples were grown in high tunnel and field environments at the kansas state university horticulture research center located in olathe, kansas. highly trained descriptive pan ... | 2010 | 20355138 |
| effect of atmosphere composition on the quality of ready-to-use broccoli raab (brassica rapa l.). | many leafy brassicas are widely used for ready-to-use salads. broccoli raab (brassica rapa l.), also called turnip top, or rapini, is extensively cultivated in southern italy. the edible portion is made up of the green, immature inflorescences and the stem with its most tender leaves. recently, interest in this crop has increased among european consumers; moreover, a substantial increase in consumption could come from the ready-to-use product. | 2010 | 20355114 |
| molecular characterization and phylogeny of a phytoplasma associated with phyllody disease of toria (brassica rapa l. subsp. dichotoma (roxb.)) in india. | samples from toria plants (brassica rapa l. subsp. dichotoma (roxb.)) exhibiting phyllody, virescence, witches broom, extensive malformation of floral parts, formation of bladder like siliquae and flower sterility were collected from four different locations in india. sequencing and phylogenetic analysis of the 16s rrna, a part of 23s rrna, partial sec a genes, rp gene and 16s-23s intergenic spacer region indicated that the phytoplasmas associated with toria phyllody (tp) symptoms were identical ... | 2010 | 23637492 |
| phytotoxicity assay for seed production using brassica rapa l. | although pesticide drift can affect crop yield adversely, current plant testing protocols emphasize only the potential impacts on vegetative plant growth. the present study was conducted to determine whether a plant species with a short life cycle, such as brassica rapa l. wisconsin fast plants®, can be used to indicate potential effects on seed production of herbicides applied at relatively low levels (e.g., low field application rates [far]). the effects of ≤0.1 × far of aminopyralid, cloransu ... | 2010 | 20872651 |
| floral genetic architecture: an examination of qtl architecture underlying floral (co)variation across environments. | genetic correlations are expected to be high among functionally related traits and lower between groups of traits with distinct functions (e.g., reproductive vs. resource-acquisition traits). here, we explore the quantitative-genetic and qtl architecture of floral organ sizes, vegetative traits, and life history in a set of brassica rapa recombinant inbred lines within and across field and greenhouse environments. floral organ lengths were strongly positively correlated within both environments, ... | 2010 | 20837996 |
| dieldrin uptake and translocation in plants growing in hydroponic medium. | it has been known that the cucurbitaceae family takes up a large amount of persistent organic pollutants from soils and that the translocation of those compounds in cucurbits is higher than those in non-cucurbits. to understand the persistent organic pollutant uptake mechanisms of plant species, we compared the dieldrin absorption and transportation potentials of several plants in hydroponic medium. sorghum (sorghum vulgare moench), sunflower (helianthus annuus l.), soybean (glycine max), komats ... | 2010 | 20821429 |
| present or past herbivory: a screening of volatiles released from brassica rapa under caterpillar attacks as attractants for the solitary parasitoid, cotesia vestalis. | females of the solitary endoparasitoid cotesia vestalis respond to a blend of volatile organic compounds (vocs) released from plants infested with larvae of their host, the diamondback moth (plutella xylostella), which is an important pest insect of cruciferous plants. we investigated the flight response of female parasitoids to the cruciferous plant brassica rapa, using two-choice tests under laboratory conditions. the parasitoids were more attracted to plants that had been infested for at leas ... | 2010 | 20490899 |
| [structural and cytochemical aspects of brassica rapa embryogenesis under conditions of clinorotation]. | the results of study of embryo development in b. rapa plants, as well as the rate and the character of nutrient substances accumulation in their cells under slow horizontal clinorotation and laboratory control are presented. significant similarity of the peculiarities of embryo differentiation and character of nutrient substance accumulation in both variants was shown. the cases of different deviations during embryo differentiation, and rate and quantity of reserve nutrient substances in their c ... | 2010 | 20480807 |
| purification and characterization of a peroxidase isozyme from indian turnip roots. | a peroxidase isozyme (tp i) from indian turnip roots ( brassica rapa ) was purified. tp i had a minimum molecular mass of 45 000 da as determined from sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). a far-uv circular dichroism (cd) spectroscopy study of tp i revealed the presence of 44% alpha-helix, 16% beta-sheet, and 40% random structure. the n-terminal sequence of tp i was found to be gln-phe-val-ile-pro-thr-tyr-ala-trp-gln. pyromellitic dianhydride (pmda)-modified tp i ... | 2010 | 20405843 |
| epigenetic chromatin modifications in brassica genomes. | epigenetic modifications such as histone and dna methylation are highly conserved among eukaryotes, although the nuclear patterns of these modifications vary between different species. brassica species represent a very attractive model for analysis of epigenetic changes because of their differences in genome size, ploidy level, and the organization of heterochromatin blocks. brassica rapa and b. oleracea are diploid species, and b. napus is an allotetraploid species that arose from the hybridiza ... | 2010 | 20237597 |
| ectopic expression of a chinese cabbage brargos gene in arabidopsis increases organ size. | chinese cabbage (brassica rapa l. ssp. pekinensis) is a common and economically important crop in asia. specific targets of plant breeding programs for cabbage have been improvement in yield, resistance to environment stresses, and nutrition quality by means of genetic manipulation. to obtain information on yield improvement applicable for the genetic engineering approach, we have attempted to dissect the molecular pathways that regulate organ size. we first isolated a putative homolog of argos ... | 2010 | 19789991 |
| relation between developmental stage, sensory properties, and volatile content of organically and conventionally grown pac choi (brassica rapa var. mei qing choi). | this study was conducted to identify and quantify the sensory characteristics and chemical profile of organically and conventionally grown pac choi (brassica rapa var. mei qing choi), also called bok choy, at 3 stages of growth (2.5, 4.5, and 6.5 wk). sensory and instrumental data were correlated using partial least squares regression. pac choi was grown in late spring. descriptive sensory analysis was conducted by a highly trained panel and compounds were identified and quantified using a gas c ... | 2010 | 20546419 |
| characterization of seedling proteomes and development of markers to distinguish the brassica a and c genomes. | the diploid species brassica rapa (genome aa) and b. oleracea (genome cc) were compared by full-scale proteome analyses of seedling. a total of 28.2% of the proteins was common to both species, indicating the existence of a basal or ubiquitous proteome. however, a number of discriminating proteins (32.0%) and specific proteins (39.8%) of the brassica a and c genomes, respectively, were identified, which could represent potentially species-specific functions. based on these a or c genome-specific ... | 2010 | 20513634 |
| genome-wide discovery of dna polymorphism in brassica rapa. | single nucleotide polymorphisms (snps) and/or insertion/deletions (indels) are frequent sequence variations in the plant genome, which can be developed as molecular markers for genetic studies on crop improvement. the ongoing brassica rapa genome sequencing project has generated vast amounts of sequence data useful in genetic research. here, we report a genome-wide survey of dna polymorphisms in the b. rapa genome based on the 557 bacterial artificial clone sequences of b. rapa ssp. pekinensis c ... | 2010 | 20024583 |
| metabolic changes of brassica rapa transformed with a bacterial isochorismate synthase gene. | metabolome analysis by 1-dimensional proton nuclear magnetic resonance (¹h nmr) coupled with multivariate data analysis was carried out in brassica rapa plants transformed with a gene encoding bacterial isochorismate synthase (ics). partial least square-discrimination analysis (pls-da) on selected signals suggested that the resonances that were dominant in the transgenic plants corresponded to a glucosinolate (neoglucobrassicin), phenylpropanoids (sinapoyl malate, feruloyl malate, caffeoyl malat ... | 2010 | 20705362 |
| the patterns of population differentiation in a brassica rapa core collection. | with the recent advances in high throughput profiling techniques the amount of genetic and phenotypic data available has increased dramatically. although many genetic diversity studies combine morphological and genetic data, metabolite profiling has yet to be integrated into these studies. for our study we selected 168 accessions representing the different morphotypes and geographic origins of brassica rapa. metabolite profiling was performed on all plants of this collection in the youngest expa ... | 2011 | 21193901 |
| developing stable progenies of ×brassicoraphanus, an intergeneric allopolyploid between brassica rapa and raphanus sativus, through induced mutation using microspore culture. | induced mutations were used to improve the low seed fertility of an intergeneric allopolyploid, 'baemoochae,' ×brassicoraphanus, synthesized following hybridization between brassica rapa and raphanus sativus. the mutagen n-methyl-n-nitroso-urethane (nmu) was added to microspore cultures. four lines of nine in the mi(2) generation showed very high fertility under controlled pollination. the progeny lines (mi(3)) confirmed this result under open pollination, and excellent uniformity was observed i ... | 2011 | 21113703 |
| evaluation of the joint effect of glyphosate and dimethoate using a small-scale terrestrial ecosystem. | in the present work a small-scale terrestrial ecosystem (stem) containing a soil collected from an agricultural field in central portugal was used to evaluate the effects of the combination of the herbicide glyphosate and the insecticide dimethoate. earthworms (eisenia andrei), isopods (porcellionides pruinosus), turnip seeds (brassica rapa), and bait-lamina strips were placed in the stem. the results showed that the application of the recommended field dose of both pesticides did not cause any ... | 2011 | 21724258 |
| evolutionary ecology of plant-microbe interactions: soil microbial structure alters selection on plant traits. | • below-ground microbial communities influence plant diversity, plant productivity, and plant community composition. given these strong ecological effects, are interactions with below-ground microbes also important for understanding natural selection on plant traits? • here, we manipulated below-ground microbial communities and the soil moisture environment on replicated populations of brassica rapa to examine how microbial community structure influences selection on plant traits and mediates pl ... | 2011 | 21658184 |
| identification of potential micrornas and their targets in brassica rapa l. | micrornas (mirnas) are recently discovered, noncoding, small regulatory rna molecules that negatively regulate gene expression. although many mirnas are identified and validated in many plant species, they remain largely unknown in brassica rapa (aa 2n =, 20). b. rapa is an important brassica crop with wide genetic and morphological diversity resulting in several subspecies that are largely grown for vegetables, oilseeds, and fodder crop production. in this study, we identified 186 mirnas belong ... | 2011 | 21647586 |
| differences in volatile profiles of turnip plants subjected to single and dual herbivory above- and belowground. | plants attacked by herbivorous insects emit volatile organic compounds that are used by natural enemies to locate their host or prey. the composition of the blend is often complex and specific. it may vary qualitatively and quantitatively according to plant and herbivore species, thus providing specific information for carnivorous arthropods. most studies have focused on simple interactions that involve one species per trophic level, and typically have investigated the aboveground parts of plant ... | 2011 | 21448706 |
| madmax - management and analysis database for multiple ~omics experiments. | the rapid increase of ~omics datasets generated by microarray, mass spectrometry and next generation sequencing technologies requires an integrated platform that can combine results from different ~omics datasets to provide novel insights in the understanding of biological systems. madmax is designed to provide a solution for storage and analysis of complex ~omics datasets. in addition, analysis results (such as lists of genes) will be merged to reveal candidate genes supported by all datasets. ... | 2011 | 21778530 |
| effect of genotype and environmental conditions on health-promoting compounds in brassica rapa. | it is well-known that a variety of factors (genetic and environmental) affect the ultimate metabolite levels in brassica vegetables, although there is still little information about the role that genetics and environment play on glucosinolates and phenolic levels. total glucosinolates were more abundant in turnip tops (26.02 μmol g(-1) dw) than in turnip greens (17.78 μmol g(-1) dw). on the other hand, total phenolic content was found in higher quantities in turnip greens (43.81 μmol g(-1) dw) t ... | 2011 | 21341668 |
| genic microsatellite markers in brassica rapa: development, characterization, mapping, and their utility in other cultivated and wild brassica relatives. | genic microsatellite markers, also known as functional markers, are preferred over anonymous markers as they reveal the variation in transcribed genes among individuals. in this study, we developed a total of 707 expressed sequence tag-derived simple sequence repeat markers (est-ssrs) and used for development of a high-density integrated map using four individual mapping populations of b. rapa. this map contains a total of 1426 markers, consisting of 306 est-ssrs, 153 intron polymorphic markers, ... | 2011 | 21768136 |
| the genetic architecture of ecophysiological and circadian traits in brassica rapa. | developmental mechanisms that enable perception of and response to the environment may enhance fitness. ecophysiological traits typically vary depending on local conditions and contribute to resource acquisition and allocation, yet correlations may limit adaptive trait expression. notably, photosynthesis and stomatal conductance vary diurnally, and the circadian clock, which is an internal estimate of time that anticipates diurnal light/dark cycles, may synchronize physiological behaviors with e ... | 2011 | 21750258 |
| development of full-length cdnas from chinese cabbage (brassica rapa subsp. pekinensis) and identification of marker genes for defence response. | arabidopsis belongs to the brassicaceae family and plays an important role as a model plant for which researchers have developed fine-tuned genome resources. genome sequencing projects have been initiated for other members of the brassicaceae family. among these projects, research on chinese cabbage (brassica rapa subsp. pekinensis) started early because of strong interest in this species. here, we report the development of a library of chinese cabbage full-length cdna clones, the riken brc b. r ... | 2011 | 21745830 |
| combined effects of soil moisture and carbaryl to earthworms and plants: simulation of flood and drought scenarios. | studying tolerance limits in organisms exposed to climatic variations is key to understanding effects on behaviour and physiology. the presence of pollutants may influence these tolerance limits, by altering the toxicity or bioavailability of the chemical. in this work, the plant species brassica rapa and triticum aestivum and the earthworm eisenia andrei were exposed to different levels of soil moisture and carbaryl, as natural and chemical stressors, respectively. both stress factors were test ... | 2011 | 21514022 |
| enhanced glucosinolates in root exudates of brassica rapa ssp. rapa mediated by salicylic acid and methyl jasmonate. | elicitation studies with salicylic acid (sa) and methyl jasmonate (mj) inducing a targeted rhizosecretion of high levels of anticarcinogenic glucosinolates in brassica rapa ssp. rapa plants were conducted. elicitor applications not only led to an accumulation of individual indole glucosinolates and the aromatic 2-phenylethyl glucosinolate in the turnip organs but also in turnip root exudates. this indicates an extended systemic response, which comprises the phyllosphere with all aboveground plan ... | 2011 | 21265537 |
| plasticity and evolution in drought avoidance and escape in the annual plant brassica rapa. | a key question in ecological genetics is to what extent do plants adapt to changes in climatic conditions, such as drought, through plasticity or evolution. to address this question, seeds of 140 maternal families of brassica rapa were generated from collections made before (1997) and after (2004) a natural drought. these seeds were planted in the glasshouse and grown under low-water and high-water conditions. post-drought lines flowered earlier than pre-drought lines, showing an evolutionary sh ... | 2011 | 21210818 |
| analysis of target sequences of ddm1s in brassica rapa by msap. | dna methylation is an important epigenetic modification regulating gene expression and transposon silencing. although epigenetic regulation is involved in some agricultural traits, there has been relatively little research on epigenetic modifications of genes in brassica rapa, which includes many important vegetables. in b. rapa, orthologs of ddm1, a chromatin remodeling factor required for maintenance of dna methylation, have been characterized and dna hypomethylated knock-down plants by rnai ( ... | 2011 | 21072521 |
| the quantitative-genetic and qtl architecture of trait integration and modularity in brassica rapa across simulated seasonal settings. | within organisms, groups of traits with different functions are frequently modular, such that variation among modules is independent and variation within modules is tightly integrated, or correlated. here, we investigated patterns of trait integration and modularity in brassica rapa in response to three simulated seasonal temperature/photoperiod conditions. the goals of this research were to use trait correlations to understand patterns of trait integration and modularity within and among floral ... | 2011 | 20736971 |
| host plant suitability and feeding preferences of the grapevine pest abagrotis orbis (lepidoptera: noctuidae). | thirteen plant species were tested for their suitability as hosts for abagrotis orbis (grote), a climbing cutworm pest of grapevines in british columbia. choice tests were also conducted to investigate larval feeding preferences for the brassicaceae species joi choi, brassica rapa variety. chinensis l., spring draba; draba verna l.; and shepherd's purse, capsella bursa-pastoris (l.) medik; compared with postdormant buds of grape, vitis vinifera l. (vitaceae), and leaves of nine other plant speci ... | 2011 | 22217761 |
| identification of conserved micrornas and their targets in chinese cabbage (brassica rapa subsp. pekinensis). | the micrornas (mirnas) are a new class of small nonprotein-coding rnas that have been identified to regulate gene expression at the post-transcriptional level by targeting mrnas for degradation or by inhibiting protein translation. until now, thousands of mirnas have been identified in many plants species. however, only 23 mirnas have been reported from the microrna database in chinese cabbage (brassica rapa subsp. pekinensis), one of the most widely cultivated vegetables in china and east asia. ... | 2011 | 22111519 |
| [ecotoxicological effects of tetracycline on the seedling development of chinese white cabbage (brassica rapa l. chinensis group.) in soil]. | the ecotoxicological effects of tetracycline (tc) on the early growth and seedlings development of chinese white cabbage were investigated using the pot-culture method. the results showed that the stem elongation of the cabbages was promoted, while the root elongation and fresh weight of the cabbages were inhibited by 1-100 mg/kg tc. after a 28-day exposure, the inhibition rate of root elongation was up to 36.0% and that of fresh weight was up to 34.6% stressed by 100 mg/kg tc. with the exposure ... | 2011 | 22619974 |
| mitochondrial genome sequencing helps show the evolutionary mechanism of mitochondrial genome formation in brassica. | angiosperm mitochondrial genomes are more complex than those of other organisms. analyses of the mitochondrial genome sequences of at least 11 angiosperm species have showed several common properties; these cannot easily explain, however, how the diverse mitotypes evolved within each genus or species. we analyzed the evolutionary relationships of brassica mitotypes by sequencing. | 2011 | 21988783 |