Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| impact of biologically synthesized silver nanoparticles on the growth and physiological responses in brassica rapa ssp. pekinensis. | silver nanoparticles (agnps) were extensively used in various fields, particularly in medicine as an antimicrobial agent. the unavoidable and extensive usage of agnps in turn accumulates in the environment. plants are the essential base of ecosystem and are ready to disturb by environmental pollutants. therefore, in the present study, we have planned to evaluate the impact of biologically synthesized agnps on the essential food crop chinese cabbage (brassica rapa ssp. pekinensis). the effects of ... | 2015 | 26154034 |
| floral volatiles interfere with plant attraction of parasitoids: ontogeny-dependent infochemical dynamics in brassica rapa. | the role of plant ontogeny on investment in direct defense against herbivores is well accepted, but the transition from the vegetative to the reproductive stage can also affect indirect resistance traits (i.e. attraction of the natural enemies of plant attackers). here, we conducted behavioral bioassays in olfactometers to determine whether the developmental stage (vegetative, pre-flowering, and flowering) of brassica rapa plants affects attraction of cotesia glomerata, a parasitoid of the herbi ... | 2015 | 26031290 |
| molecular characterization of bzr transcription factor family and abiotic stress induced expression profiling in brassica rapa. | brassinazole-resistant (bzr) transcription factors (tfs) are primarily well known as positive regulators of brassinosteroid (br) signal transduction in different plants. br is a plant specific steroid hormone, which has multiple stress resistance functions besides various growth regulatory roles. being an important regulator of the br synthesis, bzr tfs might have stress resistance related activities. however, no stress resistance related functional study of bzr tfs has been reported in any crop ... | 2015 | 25931321 |
| hard and soft selection on phenology through seasonal shifts in the general and social environments: a study on plant emergence time. | the timing of transition out of one life-history phase determines where in the seasonal succession of environments the next phase is spent. shifts in the general environment (e.g., seasonal climate) affect the expected fitness for particular transition dates. variation in transition date also leads to temporal variation in the social environment. for instance, early transition may confer a competitive advantage over later individuals. if so, the social environment will impose frequency- and dens ... | 2015 | 25929822 |
| digital gene expression analysis of gene expression differences within brassica diploids and allopolyploids. | brassica includes many successfully cultivated crop species of polyploid origin, either by ancestral genome triplication or by hybridization between two diploid progenitors, displaying complex repetitive sequences and transposons. the u's triangle, which consists of three diploids and three amphidiploids, is optimal for the analysis of complicated genomes after polyploidization. next-generation sequencing enables the transcriptome profiling of polyploids on a global scale. | 2015 | 25623840 |
| genome-wide analysis of the r2r3-myb transcription factor genes in chinese cabbage (brassica rapa ssp. pekinensis) reveals their stress and hormone responsive patterns. | the myb superfamily is one of the most abundant transcription factor (tf) families in plants. myb proteins include highly conserved n-terminal myb repeats (1r, r2r3, 3r, and atypical) and various c-terminal sequences that confer extensive functions. however, the functions of most myb genes are unknown, and have been little studied in chinese cabbage. | 2015 | 25613160 |
| high-throughput genotyping for species identification and diversity assessment in germplasm collections. | germplasm collections provide an extremely valuable resource for breeders and researchers. however, misclassification of accessions by species often hinders the effective use of these collections. we propose that use of high-throughput genotyping tools can provide a fast, efficient and cost-effective way of confirming species in germplasm collections, as well as providing valuable genetic diversity data. we genotyped 180 brassicaceae samples sourced from the australian grains genebank across the ... | 2015 | 25641370 |
| on the potential strength and consequences for nonrandom gene flow caused by local adaptation in flowering time. | gene flow is generally considered a random process, that is the loci under consideration have no effect on dispersal success. edelaar and bolnick (trends ecol evol, 27, 2012 659) recently argued that nonrandom gene flow could exert a significant evolutionary force. it can, for instance, ameliorate the maladaptive effects of immigration into locally adapted populations. i examined the potential strength for nonrandom gene flow for flowering time genes, a trait frequently found to be locally adapt ... | 2015 | 25728931 |
| selenium, putrescine, and cadmium influence health-promoting phytochemicals and molecular-level effects on turnip (brassica rapa ssp. rapa). | the effects of selenium, putrescine, and cadmium on the contents of glucosinolates, total phenolics, flavonoids, carotenoids, chlorophyll, anthocyanin, malondialdehyde, hydrogen peroxide, and antioxidant capacities as well as gene regulation of phenolics, flavonoids, carotenoids, and glucosinolates biosynthesis were investigated in turnip plants. selenium dioxide (seo2) treatment significantly induced the amount of gluconasturtiin, glucobrassicanapin, glucoallysin, glucobrassicin, 4-methoxygluco ... | 2015 | 25466011 |
| evolutionary genomics of miniature inverted-repeat transposable elements (mites) in brassica. | miniature inverted-repeat transposable elements (mites) are truncated derivatives of autonomous dna transposons, and are dispersed abundantly in most eukaryotic genomes. we aimed to characterize various mites families in brassica in terms of their presence, sequence characteristics and evolutionary activity. dot plot analyses involving comparison of homoeologous bacterial artificial chromosome (bac) sequences allowed identification of 15 novel families of mobile mites. of which, 5 were stowaway- ... | 2015 | 26129767 |
| impact of antimicrobial lipopeptides from bacillus sp. on suppression of fusarium yellows of tatsoi. | iturin a and surfactin are antimicrobial lipopeptides produced by antagonistic bacillus spp. we herein demonstrated that both lipopeptides amended the soil-mediated suppression of the soil-borne disease, fusarium yellows of tatsoi (brassica rapa var. rosularis). significant disease suppression was conferred by the amendments of purified iturin a or surfactin to soil. however, an excess amount of iturin a or surfactin to soil resulted in the loss of disease suppression activity. | 2015 | 26118972 |
| comprehensive analysis of the flowering genes in chinese cabbage and examination of evolutionary pattern of co-like genes in plant kingdom. | in plants, flowering is the most important transition from vegetative to reproductive growth. the flowering patterns of monocots and eudicots are distinctly different, but few studies have described the evolutionary patterns of the flowering genes in them. in this study, we analysed the evolutionary pattern, duplication and expression level of these genes. the main results were as follows: (i) characterization of flowering genes in monocots and eudicots, including the identification of family-sp ... | 2015 | 26416765 |
| integrative analysis of mrna and mirna expression profiles of the tuberous root development at seedling stages in turnips. | the tuberous root of brassica rapa l. (turnip) is an important modified organ for nutrition storage. a better understanding of the molecular mechanisms involved in the process of tuberous root development is of great value in both economic and biological context. in this study, we analyzed the expression profiles of both mrnas and mirnas in tuberous roots at an early stage before cortex splitting (es), cortex splitting stage (css), and secondary root thickening stage (rts) in turnip based on hig ... | 2015 | 26367742 |
| carbaryl toxicity prediction to soil organisms under high and low temperature regimes. | many studies on risk assessment of pesticides on non-target organisms have been performed based on standardized protocols that reflect conditions in temperate climates. however, the responses of organisms to chemical compounds may differ according to latitude and thus predicting the toxicity of chemicals at different temperatures is an important factor to consider in risk assessment. the toxic effects of the pesticide carbaryl were evaluated at different temperature regimes, which are indicative ... | 2015 | 24836932 |
| genome-wide analysis of the mads-box gene family in brassica rapa (chinese cabbage). | the mads-box gene family is an ancient and well-studied transcription factor family that functions in almost every developmental process in plants. there are a number of reports about the mads-box family in different plant species, but systematic analysis of the mads-box transcription factor family in brassica rapa (chinese cabbage) is still lacking. in this study, 160 mads-box transcription factors were identified from the entire chinese cabbage genome and compared with the mads-box factors fro ... | 2015 | 25216934 |
| antioxidant response and carboxylate metabolism in brassica rapa exposed to different external zn, ca, and mg supply. | zinc (zn), calcium (ca), and magnesium (mg) malnutrition are common deficiencies in many developed and developing countries, resulting in a widespread health problem. biofortification of food crops is an agricultural strategy that can be used to increase the levels of these elements in the edible portions of crops. deficiency or toxicity of these cations in soils reduces plant growth, crop yield, and the quality of plant foodstuff. the aim of this study was to investigate the effect of external ... | 2015 | 25544655 |
| anthocyanin biosynthesis for cold and freezing stress tolerance and desirable color in brassica rapa. | flavonoids are divided into several structural classes, including anthocyanins, which provide flower and leaf colors and other derivatives that play diverse roles in plant development and interactions with the environment. this study characterized four anthocyanidin synthase (ans) genes of brassica rapa, a structural gene of the anthocyanin biosynthetic pathway, and investigated their association with pigment formation, cold and freezing tolerance in b. rapa. sequences of these genes were analyz ... | 2015 | 25504198 |
| the high-throughput sequencing of small rnas profiling in wide hybridisation and allopolyploidisation between brassica rapa and brassica nigra. | small rnas play an important role in maintaining the genome reconstruction and stability in the plant. however, little is known regarding the role of small rnas during the process of wide hybridisation and chromosome doubling. therefore, the changes in the small rnas were assessed during the formation of an allodiploid (genome: ab) and its allotetraploid (genome: aabb) between brassica rapa (♀) and brassica nigra (♂) in the present study. here, the experimental methods described in details, rna- ... | 2015 | 26484138 |
| management of yellowmargined leaf beetle microtheca ochroloma (coleoptera: chrysomelidae) using turnip as a trap crop. | the yellowmargined leaf beetle, microtheca ochroloma stål, is a major pest of cruciferous vegetable crops in organic production systems. very few organically acceptable management options are currently available for this pest. field studies were conducted at a research station in alabama and at a commercial organic vegetable farm in florida to investigate the effectiveness of turnip, brassica rapa rapa, as a trap crop for m. ochroloma. in the research station trial with cabbage planted as the ca ... | 2015 | 26470380 |
| dissecting the complex molecular evolution and expression of polygalacturonase gene family in brassica rapa ssp. chinensis. | polygalacturonases (pgs) participate in pectin disassembly of cell wall and belong to one of the largest hydrolase families in plants. in this study, we identified 99 pg genes in brassica rapa. comprehensive analysis of phylogeny, gene structures, physico-chemical properties and coding sequence evolution demonstrated that plant pgs should be classified into seven divergent clades and each clade's members had specific sequence and structure characteristics, and/or were under specific selection pr ... | 2015 | 26506823 |
| genome-wide identification, classification, and expression analysis of shsp genes in chinese cabbage (brassica rapa ssp pekinensis). | small heat shock proteins (shsps) are essential for the plant's normal development and stress responses, especially the heat stress response. the information regarding shsp genes in chinese cabbage (brassica rapa ssp pekinensis) is sparse, hence we performed a genome-wide analysis to identify shsp genes in this species. we identified 26 non-redundant shsp genes distributed on all chromosomes, except chromosome a7, with one additional shsp gene identified from an expressed sequence tag library. c ... | 2015 | 26505345 |
| cloning and characterization of the nicotianamine synthase gene in eruca vesicaria subsp sativa. | nicotianamine (na) is a ubiquitous metabolite in plants that bind heavy metals, is crucial for metal homeostasis, and is also an important metal chelator that facilitates long-distance metal transport and sequestration. na synthesis is catalyzed by the enzyme nicotianamine synthase (nas). eruca vesicaria subsp sativa is highly tolerant to ni, pb, and zn. in this study, a gene encoding evnas was cloned and characterized in e. vesicaria subsp sativa. the full-length evnas cdna sequence contained a ... | 2015 | 26782459 |
| characterization and development of est-ssrs by deep transcriptome sequencing in chinese cabbage (brassica rapa l. ssp. pekinensis). | simple sequence repeats (ssrs) are among the most important markers for population analysis and have been widely used in plant genetic mapping and molecular breeding. expressed sequence tag-ssr (est-ssr) markers, located in the coding regions, are potentially more efficient for qtl mapping, gene targeting, and marker-assisted breeding. in this study, we investigated 51,694 nonredundant unigenes, assembled from clean reads from deep transcriptome sequencing with a solexa/illumina platform, for id ... | 2015 | 26504770 |
| genome-wide dna methylation profiling by modified reduced representation bisulfite sequencing in brassica rapa suggests that epigenetic modifications play a key role in polyploid genome evolution. | brassica rapa includes some of the most important vegetables worldwide as well as oilseed crops. the complete annotated genome sequence confirmed its paleohexaploid origins and provides opportunities for exploring the detailed process of polyploid genome evolution. we generated a genome-wide dna methylation profile for b. rapa using a modified reduced representation bisulfite sequencing (rrbs) method. this sampling represented 2.24% of all cg loci (2.5 × 10(5)), 2.16% chg (2.7 × 10(5)), and 1.68 ... | 2015 | 26500672 |
| glucosinolate accumulation and related gene expression in pak choi (brassica rapa l. ssp. chinensis var. communis [n. tsen & s.h. lee] hanelt) in response to insecticide application. | glucosinolates and their breakdown products are well-known for their cancer-chemoprotective functions and biocidal activities against pathogens and generalist herbivores. insecticides are commonly used in the production of pak choi (brassica rapa l. ssp. chinensis var. communis [n. tsen & s.h. lee] hanelt). we studied the effects of four commonly used insecticides, namely, β-cypermethrin, acephate, pymetrozine, and imidacloprid, on glucosinolate metabolism in pak choi. all insecticides significa ... | 2015 | 26485123 |
| quantitative trait locus analysis of seed germination and seedling vigor in brassica rapa reveals qtl hotspots and epistatic interactions. | the genetic basis of seed germination and seedling vigor is largely unknown in brassica species. we performed a study to evaluate the genetic basis of these important traits in a b. rapa doubled haploid population from a cross of a yellow-seeded oil-type yellow sarson and a black-seeded vegetable-type pak choi. we identified 26 qtl regions across all 10 linkage groups for traits related to seed weight, seed germination and seedling vigor under non-stress and salt stress conditions illustrating t ... | 2015 | 26648948 |
| variation in the flowering time orthologs brflc and brsoc1 in a natural population of brassica rapa. | understanding the genetic basis of natural phenotypic variation is of great importance, particularly since selection can act on this variation to cause evolution. we examined expression and allelic variation in candidate flowering time loci in brassica rapa plants derived from a natural population and showing a broad range in the timing of first flowering. the loci of interest were orthologs of the arabidopsis genes flc and soc1 (brflc and brsoc1, respectively), which in arabidopsis play a centr ... | 2015 | 26644966 |
| analysis of genomic dna methylation and gene expression in chinese cabbage (brassica rapa l. ssp. pekinensis) after continuous seedling breeding. | vernalization plays a key role in the bolting and flowering of chinese cabbage (brassica rapa l. ssp. pekinensis). plants can switch from vegetative to reproductive growth and then bolt and flower under low temperature induction. the economic benefits of chinese cabbage will decline significantly when the bolting happens before the vegetative body fully grows due to a lack of the edible value. it was found that continuous seedling breeding reduced the heading of chinese cabbage and led to bolt a ... | 2015 | 26601490 |
| functional divergence of bak1 genes from brassica rapa in regulating plant architecture. | bak1 is a co-receptor of bri1 in early signaling pathways mediated by brassinosteroids (brs) and is thought to play a major role in plant growth and development. as the role of bak1 has not yet been fully elucidated then further research is required to explore its potential for use in genetic modification to improve crops. in this study, three bak1 genes from the amphidiploid species brassica rapa were isolated and their kinase functions were predicted following dna sequence analysis. a bioinfor ... | 2015 | 26600518 |
| genome-wide analysis of the sbp-box gene family in chinese cabbage (brassica rapa subsp. pekinensis). | the squamosa promoter binding protein (sbp)-box gene family contains highly conserved plant-specific transcription factors that play an important role in plant development, especially in flowering. chinese cabbage (brassica rapa subsp. pekinensis) is a leafy vegetable grown worldwide and is used as a model crop for research in genome duplication. the present study aimed to characterize the sbp-box transcription factor genes in chinese cabbage. twenty-nine sbp-box genes were identified in the chi ... | 2015 | 26599708 |
| identification and expression analysis of cold and freezing stress responsive genes of brassica oleracea. | cold and freezing stress is a major environmental constraint to the production of brassica crops. enhancement of tolerance by exploiting cold and freezing tolerance related genes offers the most efficient approach to address this problem. cold-induced transcriptional profiling is a promising approach to the identification of potential genes related to cold and freezing stress tolerance. in this study, 99 highly expressed genes were identified from a whole genome microarray dataset of brassica ra ... | 2015 | 25445291 |
| comparative analysis of disease-linked single nucleotide polymorphic markers from brassica rapa for their applicability to brassica oleracea. | numerous studies using single nucleotide polymorphisms (snps) have been conducted in humans, and other animals, and in major crops, including rice, soybean, and chinese cabbage. however, the number of snp studies in cabbage is limited. in this present study, we evaluated whether 7,645 snps previously identified as molecular markers linked to disease resistance in the brassica rapa genome could be applied to b. oleracea. in a blast analysis using the snp sequences of b. rapa and b. oleracea genom ... | 2015 | 25790283 |
| seeds of a possible natural hybrid between herbicide-resistant brassica napus and brassica rapa detected on a riverbank in japan. | transgenic herbicide-resistant varieties of brassica napus, or oilseed rape, from which canola oil is obtained, are imported into japan, where this plant is not commercially cultivated to a large extent. this study aimed to examine the distribution of herbicide-resistant b. napus and transgene flow to escaped populations of its closely related species, b. rapa and b. juncea. samples were collected from 12 areas near major ports through which oilseed rape imports into japan passed--kashima, chiba ... | 2015 | 22179196 |
| nmr metabolomics of ripened and developing oilseed rape (brassica napus) and turnip rape (brassica rapa). | the oilseeds of the commercially important oilseed rape (brassica napus) and turnip rape (brassica rapa) were investigated with (1)h nmr metabolomics. the compositions of ripened (cultivated in field trials) and developing seeds (cultivated in controlled conditions) were compared in multivariate models using principal component analysis (pca), partial least squares discriminant analysis (pls-da), and orthogonal partial least squares discriminant analysis (opls-da). differences in the major lipid ... | 2015 | 25442524 |
| assessing the value of imperfect biocontainment nationally: rapeseed in the united kingdom as an exemplar. | paternal biocontainment methods (pbms) act by preventing pollen-mediated transgene flow. they are compromised by transgene escape via the crop-maternal line. we therefore assess the efficacy of pbms for transgenic rapeseed (brassica napus) biocontainment across the united kingdom by estimating crop-maternal hybridization with its two progenitor species. we used remote sensing, field surveys, agricultural statistics, and meta-analysis to determine the extent of sympatry between the crop and popul ... | 2015 | 25367754 |
| seed bank dynamics govern persistence of brassica hybrids in crop and natural habitats. | gene flow from crops to their wild relatives has the potential to alter population growth rates and demography of hybrid populations, especially when a new crop has been genetically modified (gm). this study introduces a comprehensive approach to assess this potential for altered population fitness, and uses a combination of demographic data in two habitat types and mathematical (matrix) models that include crop rotations and outcrossing between parental species. | 2015 | 25452253 |
| transcriptome analysis of canola (brassica napus) under salt stress at the germination stage. | canola (brassica napus) is one of the most important oil crops in the world. however, its yield has been constrained by salt stress. in this study, transcriptome profiles were explored using digital gene expression (dge) at 0, 3, 12 and 24 hours after h2o (control) and nacl treatments on b. napus roots at the germination stage. comparisons of gene-expression between the control and the treatment were conducted after tag-mapping to the sequenced brassica rapa genome. the differentially expressed ... | 2015 | 25679513 |
| construction of brassica a and c genome-based ordered pan-transcriptomes for use in rapeseed genomic research. | this data article reports the establishment of the first pan-transcriptome resources for the brassica a and c genomes. these were developed using existing coding dna sequence (cds) gene models from the now-published brassica oleracea to1000 and brassica napus darmor-bzh genome sequence assemblies representing the chromosomes of these species, along with preliminary cds models from an updated brassica rapa chiifu genome sequence assembly. the b. rapa genome sequence scaffolds required splitting a ... | 2015 | 26217816 |
| identification, evolution, and expression partitioning of mirnas in allopolyploid brassica napus. | the recently published genome of brassica napus offers for the first time the opportunity to gain insights into the genomic organization and the evolution of mirnas in oilseed rape. in this study, 12 small rna libraries from two b. napus cultivars (tapidor and ningyou7) and their four double-haploid lines were sequenced, employing the newly sequenced b. napus genome, together with genomes of its progenitors brassica rapa and brassica oleracea. a total of 645 mirnas including 280 conserved and 36 ... | 2015 | 26357884 |
| atmospheric h2s and so2 as sulfur source for brassica juncea and brassica rapa: impact on the glucosinolate composition. | the impact of sulfate deprivation and atmospheric h2s and so2 nutrition on the content and composition of glucosinolates was studied in brassica juncea and b. rapa. both species contained a number of aliphatic, aromatic and indolic glucosinolates. the total glucosinolate content was more than 5.5-fold higher in b. juncea than in b. rapa, which could solely be attributed to the presence of high levels of sinigrin, which was absent in the latter species. sulfate deprivation resulted in a strong de ... | 2015 | 26579170 |
| sequence and expression variation in suppressor of overexpression of constans 1 (soc1): homeolog evolution in indian brassicas. | whole genome sequence analyses allow unravelling such evolutionary consequences of meso-triplication event in brassicaceae (∼14-20 million years ago (mya)) as differential gene fractionation and diversification in homeologous sub-genomes. this study presents a simple gene-centric approach involving microsynteny and natural genetic variation analysis for understanding suppressor of overexpression of constans 1 (soc1) homeolog evolution in brassica. analysis of microsynteny in brassica rapa homeol ... | 2015 | 26276216 |
| characterization and classification of one new cytoplasmic male sterility (cms) line based on morphological, cytological and molecular markers in non-heading chinese cabbage (brassica rapa l.). | a new non-heading chinese cabbage cms line m119a was characterized and specific molecular markers were developed to classify different cms types. one new non-heading chinese cabbage (brassica rapa l.) cytoplasmic male sterile (cms) line m119a was obtained by interspecific crosses between the recently discovered hau cms line of brassica juncea and b. rapa. furthermore, the line was characterized and compared with other five isonuclear-alloplasmic cms lines. the m119a line produced six stamens wit ... | 2015 | 25972263 |
| ecological risk assessment of a metal-contaminated area in the tropics. tier ii: detailed assessment. | this study presents data on the detailed evaluation (tier 2) of a site-specific ecological risk assessment (ssera) in a former smelter area contaminated with metals (santo amaro, bahia, brazil). combining information from three lines of evidence (loe), chemical (chemloe), ecotoxicological (ecotoxloe) and ecological (ecoloe), in the triad approach, integrated risk values were calculated to rank sites and confirm the potential risk disclosed with tier 1. risk values were calculated for the habitat ... | 2015 | 26528915 |
| the fusion of genomes leads to more options: a comparative investigation on the desulfo-glucosinolate sulfotransferases of brassica napus and homologous proteins of arabidopsis thaliana. | sulfotransferases (sots) (ec 2.8.2.-) play a crucial role in the glucosinolate (gl) biosynthesis, by catalyzing the final step of the core glucosinolate formation. in arabidopsis thaliana the three desulfo (ds)-gl sots atsot16, atsot17 and atsot18 were previously characterized, showing different affinities to ds-gls. but can the knowledge about these sots be generally transferred to other gl-synthesizing plants? it was investigated how many sots are present in the economically relevant crop plan ... | 2015 | 25827495 |
| conservation of trna and rrna 5-methylcytosine in the kingdom plantae. | post-transcriptional methylation of rna cytosine residues to 5-methylcytosine (m(5)c) is an important modification that regulates rna metabolism and occurs in both eukaryotes and prokaryotes. yet, to date, no transcriptome-wide identification of m(5)c sites has been undertaken in plants. plants provide a unique comparative system for investigating the origin and evolution of m(5)c as they contain three different genomes, the nucleus, mitochondria and chloroplast. here we use bisulfite conversion ... | 2015 | 26268215 |
| characterization and stress-induced expression analysis of alfin-like transcription factors in brassica rapa. | the alfin-like (al) transcription factors (tfs) family is involved in many developmental processes, including the growth and development of roots, root hair elongation, meristem development, etc. however, stress resistance-related function and the regulatory mechanism of these tfs have yet to be elucidated. this study identified 15 brassica rapa al (bral) tfs from brad database, analyzed the sequences and profiled their expression first time in response to fusarium oxysporum f. sp. conglutinans ... | 2015 | 25618423 |
| ethnomedicinal plants of kathua district, j&k, india. | natural products, especially those derived from plants, continue to provide new and important leads in the drug discovery process. the first step in drug discovery is to document material traditionally used to treat an ailment. documentation of such knowledge will lead to its conservation and facilitate future research on medicinal plant safety and efficacy to validate traditional use. the present study was undertaken with an aim to document the ethnomedicinal plants of kathua district. | 2015 | 26023030 |
| bees use honest floral signals as indicators of reward when visiting flowers. | pollinators visit flowers for rewards and should therefore have a preference for floral signals that indicate reward status, so called 'honest signals'. we investigated honest signalling in brassica rapa l. and its relevance for the attraction of a generalised pollinator, the bumble bee bombus terrestris (l.). we found a positive association between reward amount (nectar sugar and pollen) and the floral scent compound phenylacetaldehyde. bumble bees developed a preference for phenylacetaldehyde ... | 2015 | 25491788 |
| brassica database (brad) version 2.0: integrating and mining brassicaceae species genomic resources. | the brassica database (brad) was built initially to assist users apply brassica rapa and arabidopsis thaliana genomic data efficiently to their research. however, many brassicaceae genomes have been sequenced and released after its construction. these genomes are rich resources for comparative genomics, gene annotation and functional evolutionary studies of brassica crops. therefore, we have updated brad to version 2.0 (v2.0). in brad v2.0, 11 more brassicaceae genomes have been integrated into ... | 2015 | 26589635 |
| genome-wide analysis of the expansin gene superfamily reveals brassica rapa-specific evolutionary dynamics upon whole genome triplication. | chinese cabbage (brassica rapa subsp. pekinensis) is an economically important vegetable that has encountered four rounds of polyploidization. the fourth event, whole genome triplication (wgt), occurred after its divergence from arabidopsis. expansins (exps) are cell wall loosening proteins that participate in cell wall modification processes. in this study, the impacts of wgt on the b. rapa expansin (brexp) superfamily were evaluated. whole genome screening of b. rapa identified 32 loci coding ... | 2015 | 25325993 |
| the dynamic loss and gain of introns during the evolution of the brassicaceae. | sequence comparison allows the detailed analysis of evolution at the nucleotide and amino acid levels, but much less information is known about the structural evolution of genes, i.e. how the number, length and distribution of introns change over time. we constructed a parsimonious model for the evolutionary rate of intron loss (il) and intron gain (ig) within the brassicaceae and found that il/ig has been highly dynamic, with substantial differences between and even within lineages. the diverge ... | 2015 | 25899207 |
| selection on soil microbiomes reveals reproducible impacts on plant function. | soil microorganisms found in the root zone impact plant growth and development, but the potential to harness these benefits is hampered by the sheer abundance and diversity of the players influencing desirable plant traits. here, we report a high level of reproducibility of soil microbiomes in altering plant flowering time and soil functions when partnered within and between plant hosts. we used a multi-generation experimental system using arabidopsis thaliana col to select for soil microbiomes ... | 2015 | 25350154 |
| plant aldo-keto reductases (akrs) as multi-tasking soldiers involved in diverse plant metabolic processes and stress defense: a structure-function update. | the aldo-keto reductase (akr) superfamily comprises of a large number of primarily monomeric protein members, which reduce a broad spectrum of substrates ranging from simple sugars to potentially toxic aldehydes. plant akrs can be broadly categorized into four important functional groups, which highlight their roles in diverse plant metabolic reactions including reactive aldehyde detoxification, biosynthesis of osmolytes, secondary metabolism and membrane transport. further, multiple overlapping ... | 2015 | 25840343 |
| a transposon insertion in flowering locus t is associated with delayed flowering in brassica rapa. | long days and vernalization accelerate the transition from vegetative growth to reproductive growth in brassica rapa. bolting before plants reach the harvesting stage is a serious problem in b. rapa vegetable crop cultivation. the genetic dissection of flowering time is important for breeding of premature bolting-resistant b. rapa crops. using a recombinant inbred line (ril) population, we twice detected two major quantitative trait loci (qtls) for flowering time in two different growing seasons ... | 2015 | 26706072 |
| carotenoid biosynthetic genes in brassica rapa: comparative genomic analysis, phylogenetic analysis, and expression profiling. | carotenoids are isoprenoid compounds synthesized by all photosynthetic organisms. despite much research on carotenoid biosynthesis in the model plant arabidopsis thaliana, there is a lack of information on the carotenoid pathway in brassica rapa. to better understand its carotenoid biosynthetic pathway, we performed a systematic analysis of carotenoid biosynthetic genes at the genome level in b. rapa. | 2015 | 26138916 |
| genome-wide survey and expression analysis of the pub family in chinese cabbage (brassica rapa ssp. pekinesis). | u-box proteins are widely distributed among eukaryotic organisms and show a higher prevalence in plants than in other organisms. plant u-box (pub) proteins play crucial regulatory roles in various developmental and physiological processes. previously, 64 and 77 pub genes have been identified in arabidopsis (arabidopsis thaliana) and rice (oryza sativa), respectively. in this study, 101 putative pub genes were identified in the chinese cabbage (brassica rapa ssp. pekinensis line chiifu-401-42) ge ... | 2015 | 26054324 |
| understanding brassicaceae evolution through ancestral genome reconstruction. | brassicaceae is a family of green plants of high scientific and economic interest, including thale cress (arabidopsis thaliana), cruciferous vegetables (cabbages) and rapeseed. | 2015 | 26653025 |
| genome-wide identification and analysis of the vq motif-containing protein family in chinese cabbage (brassica rapa l. ssp. pekinensis). | previous studies have showed that the vq motif-containing proteins in arabidopsis thaliana and oryza sativa play an important role in plant growth, development, and stress responses. however, little is known about the functions of the vq genes in brassica rapa (chinese cabbage). in this study, we performed genome-wide identification, characterization, and expression analysis of the vq genes in chinese cabbage, especially under adverse environment. we identified 57 vq genes and classified them in ... | 2015 | 26633387 |
| aba-stimulated sodog1 expression is after-ripening inhibited during early imbibition of germinating sisymbrium officinale seeds. | delay of germination 1 (atdog1) was the first gene identified as dormancy-associated, but its physiological role in germination is far from being understood. here, an orthologue of atdog1 in sisymbrium officinale (sodog1; km009050) is being reported. phylogenetically, the sodog1 gene is included into the dicotyledonous group together with dog1 from arabidopsis thaliana (ef028470), brassica rapa (ac189537), lepidium papillosum (jx512183, jx512185) and lepidium sativum (gq411192). the sodog1 expre ... | 2015 | 26046653 |
| genome-wide identification of aquaporin encoding genes in brassica oleracea and their phylogenetic sequence comparison to brassica crops and arabidopsis. | aquaporins (aqps) are essential channel proteins that regulate plant water homeostasis and the uptake and distribution of uncharged solutes such as metalloids, urea, ammonia, and carbon dioxide. despite their importance as crop plants, little is known about aqp gene and protein function in cabbage (brassica oleracea) and other brassica species. the recent releases of the genome sequences of b. oleracea and brassica rapa allow comparative genomic studies in these species to investigate the evolut ... | 2015 | 25904922 |
| diversification and evolution of the sdg gene family in brassica rapa after the whole genome triplication. | histone lysine methylation, controlled by the set domain group (sdg) gene family, is part of the histone code that regulates chromatin function and epigenetic control of gene expression. analyzing the sdg gene family in brassica rapa for their gene structure, domain architecture, subcellular localization, rate of molecular evolution and gene expression pattern revealed common occurrences of subfunctionalization and neofunctionalization in brsdgs. in comparison with arabidopsis thaliana, the brsd ... | 2015 | 26596461 |
| genomic and post-translational modification analysis of leucine-rich-repeat receptor-like kinases in brassica rapa. | among several receptor-like kinases (rlks), leucine-rich-repeat receptor-like kinases (lrr-rlks) are a major group of genes that play crucial roles in growth, development and stress responses in plant systems. given that they have several functional roles, it is important to investigate their roles in brassica rapa. in the present study, 303 lrr-rlks were identified in the genome of b. rapa and comparative phylogenetic analysis of 1213 combined lrr-rlks of b. rapa, arabidopsis thaliana, oryza sa ... | 2015 | 26588465 |
| molecular evolution, characterization, and expression analysis of snrk2 gene family in pak-choi (brassica rapa ssp. chinensis). | the sucrose non-fermenting 1-related protein kinase 2 (snrk2) family members are plant-specific serine/threonine kinases that are involved in the plant response to abiotic stress and abscisic acid (aba)-dependent plant development. further understanding of the evolutionary history and expression characteristics of these genes will help to elucidate the mechanisms of the stress tolerance in pak-choi, an important green leafy vegetable in china. thus, we investigated the evolutionary patterns, foo ... | 2015 | 26557127 |
| ectopic expression of a phytochrome b gene from chinese cabbage (brassica rapa l. ssp. pekinensis) in arabidopsis thaliana promotes seedling de-etiolation, dwarfing in mature plants, and delayed flowering. | phytochrome b (phyb) is an essential red light receptor that predominantly mediates seedling de-etiolation, shade-avoidance response, and flowering time. in this study, we isolate a full-length cdna of phyb, designated brphyb, from chinese cabbage (brassica rapa l. ssp. pekinensis), and we find that brphyb protein has high amino acid sequence similarity and the closest evolutionary relationship to arabidopsis thaliana phyb (i.e., atphyb). quantitative reverse transcription (rt)-pcr results indic ... | 2015 | 25724426 |
| duplicate gene divergence by changes in microrna binding sites in arabidopsis and brassica. | gene duplication provides large numbers of new genes that can lead to the evolution of new functions. duplicated genes can diverge by changes in sequences, expression patterns, and functions. micrornas play an important role in the regulation of gene expression in many eukaryotes. after duplication, two paralogs may diverge in their microrna binding sites, which might impact their expression and function. little is known about conservation and divergence of microrna binding sites in duplicated g ... | 2015 | 25644246 |
| genome-wide identification and phylogenic analysis of kelch motif containing acbp in brassica napus. | acyl-coa binding proteins (acbps) bind long chain acyl-coa esters with very high affinity. their possible involvement in fatty acid transportation from the plastid to the endoplasmic reticulum, prior to the formation of triacylglycerol has been suggested. four classes of acbps were identified in arabidopsis thaliana: the small acbps, the large acbps, the ankyrin repeats containing acbps and the kelch motif containing acbps. they differed in structure and in size, and showed multiple important fu ... | 2015 | 26156054 |
| construction of a reference genetic map of raphanus sativus based on genotyping by whole-genome resequencing. | this manuscript provides a genetic map of raphanus sativus that has been used as a reference genetic map for an ongoing genome sequencing project. the map was constructed based on genotyping by whole-genome resequencing of mapping parents and f 2 population. raphanus sativus is an annual vegetable crop species of the brassicaceae family and is one of the key plants in the seed industry, especially in east asia. assessment of the r. sativus genome provides fundamental resources for crop improveme ... | 2015 | 25403353 |
| heat shock factors in carrot: genome-wide identification, classification, and expression profiles response to abiotic stress. | heat shock factors (hsfs) play key roles in the response to abiotic stress in eukaryotes. in this study, 35 dchsfs were identified from carrot (daucus carota l.) based on the carrot genome database. all 35 dchsfs were divided into three classes (a, b, and c) according to the structure and phylogenetic relationships of four different plants, namely, arabidopsis thaliana, vitis vinifera, brassica rapa, and oryza sativa. comparative analysis of algae, gymnosperms, and angiosperms indicated that the ... | 2015 | 25403331 |
| stress-responsive expression patterns and functional characterization of cold shock domain proteins in cabbage (brassica rapa) under abiotic stress conditions. | although the functional roles of cold shock domain proteins (csdps) have been demonstrated during the growth, development, and stress adaptation of arabidopsis (arabidopsis thaliana), rice (oryza sativa), and wheat (triticum aestivum), the functions of csdps in other plants species, including cabbage (brassica rapa), are largely unknown. to gain insight into the roles of csdps in cabbage under stress conditions, the genes encoding csdps in cabbage were isolated, and the functional roles of csdps ... | 2015 | 26263516 |
| identification and expression analysis of wrky family genes under biotic and abiotic stresses in brassica rapa. | wrky proteins constitute one of the largest transcription factor families in higher plants, and they are involved in multiple biological processes such as plant development, metabolism, and responses to biotic and abiotic stresses. genes of this family have been well documented in response to many abiotic and biotic stresses in many plant species, but not yet against pectobacterium carotovorum subsp. carotovorum and fusarium oxysporum f.sp. conglutinans in any of the plants. moreover, potentiali ... | 2015 | 25149146 |
| priming with a double-stranded dna virus alters brassica rapa seed architecture and facilitates a defense response. | abiotic and biotic stresses alter genome stability and physiology of plants. under some stressful situations, a state of stress tolerance can be passed on to the offspring rendering them more suitable to stressful events than their parents. in plants, the exploration of transgenerational response has remained exclusive to model species, such as arabidopsis thaliana. here, we expand transgenerational research to include brassica rapa, a close relative to economically important plant canola (brass ... | 2015 | 25498335 |
| rate motifs tune auxin/indole-3-acetic acid degradation dynamics. | ubiquitin-mediated protein degradation is a common feature in diverse plant cell signaling pathways; however, the factors that control the dynamics of regulated protein turnover are largely unknown. one of the best-characterized families of e3 ubiquitin ligases facilitates ubiquitination of auxin (aux)/indole-3-acetic acid (iaa) repressor proteins in the presence of auxin. rates of auxin-induced degradation vary widely within the aux/iaa family, and sequences outside of the characterized degron ... | 2015 | 26149575 |
| transcriptome analysis of brassica rapa near-isogenic lines carrying clubroot-resistant and -susceptible alleles in response to plasmodiophora brassicae during early infection. | although plasmodiophora brassicae is one of the most common pathogens worldwide, the causal agent of clubroot disease in brassica crops, resistance mechanisms to it are still only poorly understood. to study the early defense response induced by p. brassicae infection, a global transcriptome profiling of the roots of two near-isogenic lines (nils) of clubroot-resistant (cr bjn3-2) and clubroot-susceptible (bjn3-2) chinese cabbage (brassica rapa) was performed by rna-seq. among the 42,730 unique ... | 2015 | 26779217 |
| yeast cell wall extract induces disease resistance against bacterial and fungal pathogens in arabidopsis thaliana and brassica crop. | housaku monogatari (hm) is a plant activator prepared from a yeast cell wall extract. we examined the efficacy of hm application and observed that hm treatment increased the resistance of arabidopsis thaliana and brassica rapa leaves to bacterial and fungal infections. hm reduced the severity of bacterial leaf spot and anthracnose on a. thaliana and brassica crop leaves with protective effects. in addition, gene expression analysis of a. thaliana plants after treatment with hm indicated increase ... | 2015 | 25565273 |
| two cytochromes p450 catalyze s-heterocyclizations in cabbage phytoalexin biosynthesis. | phytoalexins are abundant in edible crucifers and have important biological activities, yet no dedicated gene for their biosynthesis is known. here, we report two new cytochromes p450 from brassica rapa (chinese cabbage) that catalyze unprecedented s-heterocyclizations in cyclobrassinin and spirobrassinin biosynthesis. our results provide genetic and biochemical insights into the biosynthesis of a prominent pair of dietary metabolites and have implications for pathway discovery across >20 recent ... | 2015 | 26389737 |
| ageratum enation virus-a begomovirus of weeds with the potential to infect crops. | samples of two ageratum conyzoides, one sonchus oleraceus and one turnip (brassica rapa var. rapa) exhibiting virus-like symptoms were collected from pakistan and nepal. full-length begomovirus clones were obtained from the four plant samples and betasatellite clones from three of these. the begomovirus sequences were shown to be isolates of ageratum enation virus (aev) with greater than 89.1% nucleotide sequence identity to the 26 aev sequences available in the databases. the three betasatellit ... | 2015 | 25674770 |
| transcriptional slippage and rna editing increase the diversity of transcripts in chloroplasts: insight from deep sequencing of vigna radiata genome and transcriptome. | we performed deep sequencing of the nuclear and organellar genomes of three mungbean genotypes: vigna radiata ssp. sublobata tc1966, v. radiata var. radiata nm92 and the recombinant inbred line ril59 derived from a cross between tc1966 and nm92. moreover, we performed deep sequencing of the ril59 transcriptome to investigate transcript variability. the mungbean chloroplast genome has a quadripartite structure including a pair of inverted repeats separated by two single copy regions. a total of 2 ... | 2015 | 26076132 |
| a simple and efficient agrobacterium tumefaciens-mediated plant transformation of brassica rapa ssp. pekinensis. | the present study aims to investigate the numerous factors influencing agrobacterium tumefaciens-mediated genetic transformation of chinese cabbage (brassica rapa ssp. pekinensis). factors affecting transformation efficiency, such as age of explants, agrobacterium concentration, and effect of acetosyringone, pre-cultivation, infection and co-cultivation time of agrobacterium were examined. the pre-cultured hypocotyls from young seedlings prior to exposure to agrobacterium showed higher shoot reg ... | 2016 | 28330158 |
| genome-wide analysis and characterization of aux/iaa family genes in brassica rapa. | auxins are the key players in plant growth development involving leaf formation, phototropism, root, fruit and embryo development. auxin/indole-3-acetic acid (aux/iaa) are early auxin response genes noted as transcriptional repressors in plant auxin signaling. however, many studies focus on aux/arf gene families and much less is known about the aux/iaa gene family in brassica rapa (b. rapa). here we performed a comprehensive genome-wide analysis and identified 55 aux/iaa genes in b. rapa using f ... | 2016 | 27049520 |
| growth and (137)cs uptake and accumulation among 56 japanese cultivars of brassica rapa, brassica juncea and brassica napus grown in a contaminated field in fukushima: effect of inoculation with a bacillus pumilus strain. | fifty six local japanese cultivars of brassica rapa (40 cultivars), brassica juncea (10 cultivars) and brassica napus (6 cultivars) were assessed for variability in growth and (137)cs uptake and accumulation in association with a bacillus pumilus strain. field trial was conducted at a contaminated farmland in nihonmatsu city, in fukushima prefecture. inoculation resulted in different responses of the cultivars in terms of growth and radiocesium uptake and accumulation. b. pumilus induced a signi ... | 2016 | 26986237 |
| shotgun label-free proteomic analysis of clubroot (plasmodiophora brassicae) resistance conferred by the gene rcr1 in brassica rapa. | clubroot, caused by the plasmodiophorid pathogen plasmodiophora brassicae, is one of the most serious diseases on brassica crops worldwide and a major threat to canola production in western canada. host resistance is the key strategy for clubroot management on canola. several clubroot resistance (cr) genes have been identified, but the mechanisms associated with these cr genes are poorly understood. in the current study, a label-free shotgun proteomic approach was used to profile and compare the ... | 2016 | 27462338 |
| brassica napus genome possesses extraordinary high number of camta genes and camta3 contributes to pamp triggered immunity and resistance to sclerotinia sclerotiorum. | calmodulin-binding transcription activators (camtas) play important roles in various plant biological processes including disease resistance and abiotic stress tolerance. oilseed rape (brassica napus l.) is one of the most important oil-producing crops worldwide. to date, compositon of camtas in genomes of brassica species and role of camtas in resistance to the devastating necrotrophic fungal pathogen sclerotinia sclerotiorum are still unknown. in this study, 18 camta genes were identified in o ... | 2016 | 27200054 |
| multiple evolutionary events involved in maintaining homologs of resistance to powdery mildew 8 in brassica napus. | the resistance to powdery mildew 8 (rpw8) locus confers broad-spectrum resistance to powdery mildew in arabidopsis thaliana. there are four homologous to rpw8s (brhrs) in brassica rapa and three in brassica oleracea (bohrs). brassica napus (bn) is derived from diploidization of a hybrid between b. rapa and b. oleracea, thus should have seven homologs of rpw8 (bnhrs). it is unclear whether these genes are still maintained or lost in b. napus after diploidization and how they might have been evolv ... | 2016 | 27493652 |
| a genome-wide analysis reveals stress and hormone responsive patterns of tify family genes in brassica rapa. | the tify family is a plant-specific group of proteins with a diversity of functions and includes four subfamilies, viz. zml, tify, ppd, and jasmonate zim-domain (jaz) proteins. tify family members, particularly jaz subfamily proteins, play roles in biological processes such as development and stress and hormone responses in arabidopsis, rice, chickpea, and grape. however, there is no information about this family in any brassica crop. this study identifies 36 tify genes in brassica rapa, an econ ... | 2016 | 27446164 |
| characterization and expression profiling of myb transcription factors against stresses and during male organ development in chinese cabbage (brassica rapa ssp. pekinensis). | myb proteins comprise a large family of plant transcription factors that play regulatory roles in different biological processes such as plant development, metabolism, and defense responses. to gain insight into this gene superfamily and to elucidate its roles in stress resistance, we performed a comprehensive genome-wide identification, characterization, and expression analysis of myb genes in chinese cabbage (brassica rapa ssp. pekinensis). we identified 475 chinese cabbage myb genes, among wh ... | 2016 | 27038155 |
| genotyping-by-sequencing map permits identification of clubroot resistance qtls and revision of the reference genome assembly in cabbage (brassica oleracea l.). | clubroot is a devastating disease caused by plasmodiophora brassicae and results in severe losses of yield and quality in brassica crops. many clubroot resistance genes and markers are available in brassica rapa but less is known in brassica oleracea. here, we applied the genotyping-by-sequencing (gbs) technique to construct a high-resolution genetic map and identify clubroot resistance (cr) genes. a total of 43,821 snps were identified using gbs data for two parental lines, one resistant and on ... | 2016 | 26622061 |
| simultaneous production of l-lactic acid with high optical activity and a soil amendment with food waste that demonstrates plant growth promoting activity. | a unique method to produce highly optically-active l-lactic acid and soil amendments that promote plant growth from food waste was proposed. three bacillus strains bacillus subtilis kbku21, b. subtilis n3-9 and bacillus coagulans t27, were used. strain kbku21 accumulated 36.9 g/l l-lactic acid with 95.7% optical activity and 98.2% l-lactic acid selectivity when fermented at 43°c for 84 h in a model kitchen refuse (mkr) medium. residual precipitate fraction (anaerobically-fermented mkr (afm) comp ... | 2016 | 26819060 |
| isolate dependency of brassica rapa resistance qtls to botrytis cinerea. | generalist necrotrophic pathogens including botrytis cinerea cause significant yield and financial losses on brassica crops. however, there is little knowledge about the mechanisms underlying the complex interactions encoded by both host and pathogen genomes in this interaction. this potentially includes multiple layers of plant defense and pathogen virulence mechanisms that could complicate in breeding broad spectrum resistance within brassica species. glucosinolates (gsls) are a diverse group ... | 2016 | 26925079 |
| identification of genome-wide variants and discovery of variants associated with brassica rapa clubroot resistance gene rcr1 through bulked segregant rna sequencing. | clubroot, caused by plasmodiophora brassicae, is an important disease on brassica species worldwide. a clubroot resistance gene, rcr1, with efficacy against pathotype 3 of p. brassicae, was previously mapped to chromosome a03 of b. rapa in pak choy cultivar "flower nabana". in the current study, resistance to pathotypes 2, 5 and 6 was shown to be associated with rcr1 region on chromosome a03. bulked segregant rna sequencing was performed and short read sequences were assembled into 10 chromosome ... | 2016 | 27078023 |
| relationship of enhanced butyrate production by colonic butyrate-producing bacteria to immunomodulatory effects in normal mice fed an insoluble fraction of brassica rapa l. | this study was performed to determine the effects of feeding a fiber-rich fraction of brassica vegetables on the immune response through changes in enteric bacteria and short-chain fatty acid (scfa) production in normal mice. the boiled-water-insoluble fraction of brassica rapa l. (nozawana), which consists mainly of dietary fiber, was chosen as a test material. a total of 31 male c57bl/6j mice were divided into two groups and housed in a specific-pathogen-free facility. the animals were fed eit ... | 2016 | 26921420 |
| development of primer sets that can verify the enrichment of histone modifications, and their application to examining vernalization-mediated chromatin changes in brassica rapa l. | epigenetic regulation is crucial for the development of plants and for adaptation to a changing environment. recently, genome-wide profiles of histone modifications have been determined by a combination of chromatin immunoprecipitation (chip) and genomic tiling arrays (chip on chip) or chip and high-throughput sequencing (chip-seq) in species including arabidopsis thaliana, rice and maize. validation of chip analysis by pcr or qpcr using positive and negative regions of histone modification is n ... | 2016 | 27074983 |
| genetic variation and divergence of genes involved in leaf adaxial-abaxial polarity establishment in brassica rapa. | alterations in leaf adaxial-abaxial (ad-ab) polarity are one of the main factors that influence leaf curvature. in chinese cabbage, leaf incurvature is an essential prerequisite to the formation of a leafy head. identifying ad-ab patterning genes and investigating their genetic variation may facilitate elucidation of the mechanisms underlying leaf incurvature during head formation. comparative genomic analysis of 45 leaf ad-ab patterning genes in brassica rapa based on 26 homologs of arabidopsis ... | 2016 | 26904064 |
| bioinformatic identification and analysis of extensins in the plant kingdom. | extensins (exts) are a family of plant cell wall hydroxyproline-rich glycoproteins (hrgps) that are implicated to play important roles in plant growth, development, and defense. structurally, exts are characterized by the repeated occurrence of serine (ser) followed by three to five prolines (pro) residues, which are hydroxylated as hydroxyproline (hyp) and glycosylated. some exts have tyrosine (tyr)-x-tyr (where x can be any amino acid) motifs that are responsible for intramolecular or intermol ... | 2016 | 26918442 |
| functional analysis of three brmyb28 transcription factors controlling the biosynthesis of glucosinolates in brassica rapa. | glucosinolates (gsls) are secondary metabolites that have anticarcinogenic activity and play defense roles in plants of the brassicaceae family. myb28 is known as a transcription factor that regulates aliphatic gsl biosynthesis in arabidopsis thaliana. brassicaceae plants have three orthologous copies of atmyb28 derived from recent genome triplication. these brmyb28 genes have a high level of sequence homology, with 81-87% similarities in the coding dna sequence compared to arabidopsis. overexpr ... | 2016 | 26820138 |
| fine mapping and candidate gene analysis of brtri1, a gene controlling trichome development in chinese cabbage (brassica rapa l. ssp pekinensis). | trichomes are derived from the epidermis and constitute an ideal system for studying cell division in plants. here, a chinese cabbage doubled haploid (dh) line (ft) without trichomes was crossed with another dh line (purdh-1) with trichomes to develop an f2 population for fine mapping of trichome control genes. genetic analysis showed that the trichome phenotype was controlled by a single dominant gene, brtri1. using 1226 glabrous individuals in the f2 segregation population, brtri1 was localize ... | 2016 | 27966738 |
| patterns of substitution rate variation at many nuclear loci in two species trios in the brassicaceae partitioned with anova. | there are marked variations among loci and among lineages in rates of nucleotide substitution. the generation time hypothesis (gth) is a neutral explanation for substitution rate heterogeneity that has genomewide application, predicting that species with shorter generation times accumulate dna sequence substitutions faster than species with longer generation times do since faster genome replication provides more opportunities for mutations to occur and reach fixation by genetic drift. relatively ... | 2016 | 27592229 |
| phylogenetic analysis and photoregulation of siroheme biosynthesis genes: uroporphyrinogen iii methyltransferase and sirohydrochlorin ferrochelatase of arabidopsis thaliana. | uroporphyrinogen iii methyl transferase (upm1) and sirohydrochlorin ferrochelatase (sirb) are the important genes involved in the biosynthesis of siroheme, the prosthetic group of nitrite reductases (nir) and sulfite reductases (sir) involved in nitrogen and sulfur assimilation. both upm1 and sirb could be potential candidate genes targeted for sustainable agriculture especially in n-deficient soil. the phylogenetic analysis revealed that these genes are highly conserved among algae, bryophytes ... | 2016 | 27729721 |
| cloning and characterization of the dehydration-responsive element-binding protein 2a gene in eruca vesicaria subsp sativa. | eruca vesicaria subsp sativa is one of the most tolerant cruciferae species to drought, and dehydration-responsive element-binding protein 2a (dreb2a) is involved in responses to salinity, heat, and particularly drought. in this study, a gene encoding evdreb2a was cloned and characterized in e. vesicaria subsp sativa. the full-length evdreb2a cdna sequence contained a 388-bp 5'-untranslated region (utr), a 348-bp 3'-utr, and a 1002-bp open reading frame that encoded 334 amino acid residues. the ... | 2016 | 27525923 |
| the epidermis coordinates auxin-induced stem growth in response to shade. | growth of a complex multicellular organism requires coordinated changes in diverse cell types. these cellular changes generate organs of the correct size, shape, and functionality. in plants, the growth hormone auxin induces stem elongation in response to shade; however, which cell types of the stem perceive the auxin signal and contribute to organ growth is poorly understood. here, we blocked the transcriptional response to auxin within specific tissues to show that auxin signaling is required ... | 2016 | 27401556 |
| regulation of bolting and identification of the α-tubulin gene family in brassica rapa l. ssp pekinensis. | microtubules are important components of eukaryotic cells, and they play vital roles in cell morphogenesis, carrying of signaling molecules, transport of materials, and establishing the cell polarity. during bolting of biennial plants, cell division and elongation are involved, and cell elongation inevitably involves the microtubules arrangement and expression of related genes. so we deduce that it is of great significance to figure out the mechanism of bolting and flowering in which tua genes a ... | 2016 | 26909938 |