Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| effects of glucosinolates from turnip (brassica rapa l.) root on bone formation by human osteoblast-like mg-63 cells and in normal young rats. | turnip (brassica rapa l.) root ethanol extract (tre) was prepared, and its chemical constituents were characterized by ultra-performance liquid chromatography and mass spectrometry. thirteen glucosinolates (gsls) were identified, comprising eight aliphatic, four indolic, and one aromatic compounds. the effects of these gsls on bone formation were investigated in vitro by incubating human osteoblast-like mg-63 cells with tre and then analyzing their viability, alkaline phosphatase (alp) activity, ... | 2015 | 25809011 |
| analysis of population genetic structure and gene flow in an annual plant before and after a rapid evolutionary response to drought. | the impact of environmental change on population structure is not well understood. this study aimed to examine the effect of a climate change event on gene flow over space and time in two populations of brassica rapa that evolved more synchronous flowering times over 5 years of drought in southern california. using plants grown from seeds collected before and after the drought, we estimated genetic parameters within and between populations and across generations. we expected that with greater te ... | 2015 | 25818074 |
| the fusion of genomes leads to more options: a comparative investigation on the desulfo-glucosinolate sulfotransferases of brassica napus and homologous proteins of arabidopsis thaliana. | sulfotransferases (sots) (ec 2.8.2.-) play a crucial role in the glucosinolate (gl) biosynthesis, by catalyzing the final step of the core glucosinolate formation. in arabidopsis thaliana the three desulfo (ds)-gl sots atsot16, atsot17 and atsot18 were previously characterized, showing different affinities to ds-gls. but can the knowledge about these sots be generally transferred to other gl-synthesizing plants? it was investigated how many sots are present in the economically relevant crop plan ... | 2015 | 25827495 |
| modification of nitrate uptake pathway in plants affects the cadmium uptake by roots. | nrt1.1 is a dual-affinity nitrate (no3(-)) transporter involved in both high- and low-affinity no3(-) uptake in arabidopsis plants. in a recent study, we showed that, under cadmium (cd) exposure, blocking the nrt1.1-mediated no3(-) uptake reduces cd entry into roots, thus lowing cd levels in plants and improving plant growth. in addition, we also found that the cd levels in edible parts of 11 chinese cabbage (brassica rapa l. ssp. pekinensis) cultivars correlated well with the no3(-) uptake rate ... | 2015 | 25830329 |
| plant aldo-keto reductases (akrs) as multi-tasking soldiers involved in diverse plant metabolic processes and stress defense: a structure-function update. | the aldo-keto reductase (akr) superfamily comprises of a large number of primarily monomeric protein members, which reduce a broad spectrum of substrates ranging from simple sugars to potentially toxic aldehydes. plant akrs can be broadly categorized into four important functional groups, which highlight their roles in diverse plant metabolic reactions including reactive aldehyde detoxification, biosynthesis of osmolytes, secondary metabolism and membrane transport. further, multiple overlapping ... | 2015 | 25840343 |
| genome-wide identification, classification, and analysis of heat shock transcription factor family in chinese cabbage (brassica rapa pekinensis). | chinese cabbage (brassica rapa ssp. pekinensis) is one of the most important vegetable crops grown worldwide, and various methods exist for selection, propagation, and cultivation. the entire chinese cabbage genome has been sequenced, and the heat shock transcription factor family (hsfs) has been found to play a central role in plant growth and development and in the response to biotic and abiotic stress conditions, particularly in acquired thermotolerance. we analyzed heat tolerance mechanisms ... | 2015 | 25867366 |
| genome-wide identification and characterization of mads-box family genes related to organ development and stress resistance in brassica rapa. | mads-box transcription factors (tfs) are important in floral organ specification as well as several other aspects of plant growth and development. studies on stress resistance-related functions of mads-box genes are very limited and no such functional studies in brassica rapa have been reported. to gain insight into this gene family and to elucidate their roles in organ development and stress resistance, we performed genome-wide identification, characterization and expression analysis of mads-bo ... | 2015 | 25881193 |
| the dynamic loss and gain of introns during the evolution of the brassicaceae. | sequence comparison allows the detailed analysis of evolution at the nucleotide and amino acid levels, but much less information is known about the structural evolution of genes, i.e. how the number, length and distribution of introns change over time. we constructed a parsimonious model for the evolutionary rate of intron loss (il) and intron gain (ig) within the brassicaceae and found that il/ig has been highly dynamic, with substantial differences between and even within lineages. the diverge ... | 2015 | 25899207 |
| genome-wide identification of aquaporin encoding genes in brassica oleracea and their phylogenetic sequence comparison to brassica crops and arabidopsis. | aquaporins (aqps) are essential channel proteins that regulate plant water homeostasis and the uptake and distribution of uncharged solutes such as metalloids, urea, ammonia, and carbon dioxide. despite their importance as crop plants, little is known about aqp gene and protein function in cabbage (brassica oleracea) and other brassica species. the recent releases of the genome sequences of b. oleracea and brassica rapa allow comparative genomic studies in these species to investigate the evolut ... | 2015 | 25904922 |
| uncovering the dynamic evolution of nucleotide-binding site-leucine-rich repeat (nbs-lrr) genes in brassicaceae. | plant genomes harbor dozens to hundreds of nucleotide-binding site-leucine-rich repeat (nbs-lrr) genes; however, the long-term evolutionary history of these resistance genes has not been fully understood. this study focuses on five brassicaceae genomes and the carica papaya genome to explore changes in nbs-lrr genes that have taken place in this rosid ii lineage during the past 72 million years. various numbers of nbs-lrr genes were identified from arabidopsis lyrata (198), a. thaliana (165), br ... | 2016 | 25926337 |
| hard and soft selection on phenology through seasonal shifts in the general and social environments: a study on plant emergence time. | the timing of transition out of one life-history phase determines where in the seasonal succession of environments the next phase is spent. shifts in the general environment (e.g., seasonal climate) affect the expected fitness for particular transition dates. variation in transition date also leads to temporal variation in the social environment. for instance, early transition may confer a competitive advantage over later individuals. if so, the social environment will impose frequency- and dens ... | 2015 | 25929822 |
| molecular characterization of bzr transcription factor family and abiotic stress induced expression profiling in brassica rapa. | brassinazole-resistant (bzr) transcription factors (tfs) are primarily well known as positive regulators of brassinosteroid (br) signal transduction in different plants. br is a plant specific steroid hormone, which has multiple stress resistance functions besides various growth regulatory roles. being an important regulator of the br synthesis, bzr tfs might have stress resistance related activities. however, no stress resistance related functional study of bzr tfs has been reported in any crop ... | 2015 | 25931321 |
| effects of aqueous extract of turnip leaf (brassica rapa) in alloxan-induced diabetic rats. | turnip leaf has been used in folk medicine of iran for the treatment of diabetes. however,so far no scientific study has been done to support its use in traditional medicine. the present study was carried out to evaluate the possible hypoglycemic efficacy of aqueous extract of turnip leaf (aetl) in diabetic rats. | 2017 | 25949956 |
| characterization and classification of one new cytoplasmic male sterility (cms) line based on morphological, cytological and molecular markers in non-heading chinese cabbage (brassica rapa l.). | a new non-heading chinese cabbage cms line m119a was characterized and specific molecular markers were developed to classify different cms types. one new non-heading chinese cabbage (brassica rapa l.) cytoplasmic male sterile (cms) line m119a was obtained by interspecific crosses between the recently discovered hau cms line of brassica juncea and b. rapa. furthermore, the line was characterized and compared with other five isonuclear-alloplasmic cms lines. the m119a line produced six stamens wit ... | 2015 | 25972263 |
| quantitative trait loci × environment interactions for plant morphology vary over ontogeny in brassica rapa. | growth in plants occurs via the addition of repeating modules, suggesting that the genetic architecture of similar subunits may vary between earlier- and later-developing modules. these complex environment × ontogeny interactions are not well elucidated, as studies examining quantitative trait loci (qtls) expression over ontogeny have not included multiple environments. here, we characterized the genetic architecture of vegetative traits and onset of reproduction over ontogeny in recombinant inb ... | 2014 | 26012723 |
| ethnomedicinal plants of kathua district, j&k, india. | natural products, especially those derived from plants, continue to provide new and important leads in the drug discovery process. the first step in drug discovery is to document material traditionally used to treat an ailment. documentation of such knowledge will lead to its conservation and facilitate future research on medicinal plant safety and efficacy to validate traditional use. the present study was undertaken with an aim to document the ethnomedicinal plants of kathua district. | 2015 | 26023030 |
| floral volatiles interfere with plant attraction of parasitoids: ontogeny-dependent infochemical dynamics in brassica rapa. | the role of plant ontogeny on investment in direct defense against herbivores is well accepted, but the transition from the vegetative to the reproductive stage can also affect indirect resistance traits (i.e. attraction of the natural enemies of plant attackers). here, we conducted behavioral bioassays in olfactometers to determine whether the developmental stage (vegetative, pre-flowering, and flowering) of brassica rapa plants affects attraction of cotesia glomerata, a parasitoid of the herbi ... | 2015 | 26031290 |
| high-throughput multiplex cpdna resequencing clarifies the genetic diversity and genetic relationships among brassica napus, brassica rapa and brassica oleracea. | brassica napus (rapeseed) is a recent allotetraploid plant and the second most important oilseed crop worldwide. the origin of b. napus and the genetic relationships with its diploid ancestor species remain largely unresolved. here, chloroplast dna (cpdna) from 488 b. napus accessions of global origin, 139 b. rapa accessions and 49 b. oleracea accessions were populationally resequenced using illumina solexa sequencing technologies. the intraspecific cpdna variants and their allelic frequencies w ... | 2016 | 26031705 |
| molecular characterization of brmyb28 and brmyb29 paralogous transcription factors involved in the regulation of aliphatic glucosinolate profiles in brassica rapa ssp. pekinensis. | glucosinolates (gsl) are one of the major secondary metabolites of the brassicaceae family. in the present study, we aim at characterizing the multiple paralogs of aliphatic gsl regulators, such as brmyb28 and brmyb29 genes in brassica rapa ssp. pekinensis, by quantitative real-time pcr (qrt-pcr) analysis in different tissues and at various developmental stages. an overlapping gene expression pattern between the brmybs as well as their downstream genes (dsgs) was found at different developmental ... | 2015 | 26043798 |
| aba-stimulated sodog1 expression is after-ripening inhibited during early imbibition of germinating sisymbrium officinale seeds. | delay of germination 1 (atdog1) was the first gene identified as dormancy-associated, but its physiological role in germination is far from being understood. here, an orthologue of atdog1 in sisymbrium officinale (sodog1; km009050) is being reported. phylogenetically, the sodog1 gene is included into the dicotyledonous group together with dog1 from arabidopsis thaliana (ef028470), brassica rapa (ac189537), lepidium papillosum (jx512183, jx512185) and lepidium sativum (gq411192). the sodog1 expre ... | 2015 | 26046653 |
| inflorescences of brassicacea species as source of bioactive compounds: a comparative study. | two brassica oleracea varieties (b. oleracea l. var. costata dc and b. oleracea l. var. acephala) and brassica rapa l. var. rapa inflorescences were studied for their chemical composition and antioxidant capacity. phenolic compounds and organic acids profiles were determined by hplc-dad and hplc-uv, respectively. b. oleracea var. costata and b. oleracea l. var. acephala inflorescences presented a similar qualitative phenolic composition, exhibiting several complex kaempferol derivatives and 3-p- ... | 2008 | 26047285 |
| genome-wide survey and expression analysis of the pub family in chinese cabbage (brassica rapa ssp. pekinesis). | u-box proteins are widely distributed among eukaryotic organisms and show a higher prevalence in plants than in other organisms. plant u-box (pub) proteins play crucial regulatory roles in various developmental and physiological processes. previously, 64 and 77 pub genes have been identified in arabidopsis (arabidopsis thaliana) and rice (oryza sativa), respectively. in this study, 101 putative pub genes were identified in the chinese cabbage (brassica rapa ssp. pekinensis line chiifu-401-42) ge ... | 2015 | 26054324 |
| transcriptional slippage and rna editing increase the diversity of transcripts in chloroplasts: insight from deep sequencing of vigna radiata genome and transcriptome. | we performed deep sequencing of the nuclear and organellar genomes of three mungbean genotypes: vigna radiata ssp. sublobata tc1966, v. radiata var. radiata nm92 and the recombinant inbred line ril59 derived from a cross between tc1966 and nm92. moreover, we performed deep sequencing of the ril59 transcriptome to investigate transcript variability. the mungbean chloroplast genome has a quadripartite structure including a pair of inverted repeats separated by two single copy regions. a total of 2 ... | 2015 | 26076132 |
| hydrogen sulfide alleviates cadmium-induced cell death through restraining ros accumulation in roots of brassica rapa l. ssp. pekinensis. | hydrogen sulfide (h2s) is a cell signal molecule produced endogenously and involved in regulation of tolerance to biotic and abiotic stress in plants. in this work, we used molecular biology, physiology, and histochemical methods to investigate the effects of h2s on cadmium- (cd-) induced cell death in chinese cabbage roots. cd stress stimulated a rapid increase of endogenous h2s in roots. additionally, root length was closely related to the cell death rate. pretreatment with sodium hydrosulfide ... | 2015 | 26078819 |
| modeling development and quantitative trait mapping reveal independent genetic modules for leaf size and shape. | improved predictions of fitness and yield may be obtained by characterizing the genetic controls and environmental dependencies of organismal ontogeny. elucidating the shape of growth curves may reveal novel genetic controls that single-time-point (stp) analyses do not because, in theory, infinite numbers of growth curves can result in the same final measurement. we measured leaf lengths and widths in brassica rapa recombinant inbred lines (rils) throughout ontogeny. we modeled leaf growth and a ... | 2015 | 26083847 |
| what drives selection on flowering time? an experimental manipulation of the inherent correlation between genotype and environment. | the optimal timing of the seasonal switch from somatic growth to reproduction can depend on an individual's condition at reproduction, the quality of the environment in which it will reproduce, or both. in annual plants, vegetative size (a function of age at flowering) affects resources available for seed production, whereas exposure to mutualists, antagonists, and abiotic stresses in the environment (functions of julian date of flowering) influences success in converting resources into offsprin ... | 2015 | 26102569 |
| thin layer chromatography-autography-high resolution mass spectrometry analysis: accelerating the identification of acetylcholinesterase inhibitors. | the prevailing treatment for alzheimer's disease is the use of acetylcholinesterase (ache) inhibitors. natural extracts are the principal source of ache's inhibitors. however, their chemical complexity demands for simple, selective and rapid assays. | 2015 | 26102595 |
| global gene-expression analysis to identify differentially expressed genes critical for the heat stress response in brassica rapa. | genome-wide dissection of the heat stress response (hsr) is necessary to overcome problems in crop production caused by global warming. to identify hsr genes, we profiled gene expression in two chinese cabbage inbred lines with different thermotolerances, chiifu and kenshin. many genes exhibited >2-fold changes in expression upon exposure to 0.5- 4 h at 45°c (high temperature, ht): 5.2% (2,142 genes) in chiifu and 3.7% (1,535 genes) in kenshin. the most enriched go (gene ontology) items included ... | 2015 | 26102990 |
| construction of chromosome segment substitution lines enables qtl mapping for flowering and morphological traits in brassica rapa. | chromosome segment substitution lines (cssls) represent a powerful method for precise quantitative trait loci (qtl) detection of complex agronomical traits in plants. in this study, we used a marker-assisted backcrossing strategy to develop a population consisting of 63 cssls, derived from backcrossing of the f1 generated from a cross between two brassica rapa subspecies: "chiifu" (ssp. pekinensis), the brassica "a" genome-represented line used as the donor, and "49caixin" (ssp. parachinensis), ... | 2015 | 26106405 |
| impact of antimicrobial lipopeptides from bacillus sp. on suppression of fusarium yellows of tatsoi. | iturin a and surfactin are antimicrobial lipopeptides produced by antagonistic bacillus spp. we herein demonstrated that both lipopeptides amended the soil-mediated suppression of the soil-borne disease, fusarium yellows of tatsoi (brassica rapa var. rosularis). significant disease suppression was conferred by the amendments of purified iturin a or surfactin to soil. however, an excess amount of iturin a or surfactin to soil resulted in the loss of disease suppression activity. | 2015 | 26118972 |
| evolutionary genomics of miniature inverted-repeat transposable elements (mites) in brassica. | miniature inverted-repeat transposable elements (mites) are truncated derivatives of autonomous dna transposons, and are dispersed abundantly in most eukaryotic genomes. we aimed to characterize various mites families in brassica in terms of their presence, sequence characteristics and evolutionary activity. dot plot analyses involving comparison of homoeologous bacterial artificial chromosome (bac) sequences allowed identification of 15 novel families of mobile mites. of which, 5 were stowaway- ... | 2015 | 26129767 |
| carotenoid biosynthetic genes in brassica rapa: comparative genomic analysis, phylogenetic analysis, and expression profiling. | carotenoids are isoprenoid compounds synthesized by all photosynthetic organisms. despite much research on carotenoid biosynthesis in the model plant arabidopsis thaliana, there is a lack of information on the carotenoid pathway in brassica rapa. to better understand its carotenoid biosynthetic pathway, we performed a systematic analysis of carotenoid biosynthetic genes at the genome level in b. rapa. | 2015 | 26138916 |
| rate motifs tune auxin/indole-3-acetic acid degradation dynamics. | ubiquitin-mediated protein degradation is a common feature in diverse plant cell signaling pathways; however, the factors that control the dynamics of regulated protein turnover are largely unknown. one of the best-characterized families of e3 ubiquitin ligases facilitates ubiquitination of auxin (aux)/indole-3-acetic acid (iaa) repressor proteins in the presence of auxin. rates of auxin-induced degradation vary widely within the aux/iaa family, and sequences outside of the characterized degron ... | 2015 | 26149575 |
| impact of biologically synthesized silver nanoparticles on the growth and physiological responses in brassica rapa ssp. pekinensis. | silver nanoparticles (agnps) were extensively used in various fields, particularly in medicine as an antimicrobial agent. the unavoidable and extensive usage of agnps in turn accumulates in the environment. plants are the essential base of ecosystem and are ready to disturb by environmental pollutants. therefore, in the present study, we have planned to evaluate the impact of biologically synthesized agnps on the essential food crop chinese cabbage (brassica rapa ssp. pekinensis). the effects of ... | 2015 | 26154034 |
| genome-wide identification and phylogenic analysis of kelch motif containing acbp in brassica napus. | acyl-coa binding proteins (acbps) bind long chain acyl-coa esters with very high affinity. their possible involvement in fatty acid transportation from the plastid to the endoplasmic reticulum, prior to the formation of triacylglycerol has been suggested. four classes of acbps were identified in arabidopsis thaliana: the small acbps, the large acbps, the ankyrin repeats containing acbps and the kelch motif containing acbps. they differed in structure and in size, and showed multiple important fu ... | 2015 | 26156054 |
| accumulation of phenylpropanoids by white, blue, and red light irradiation and their organ-specific distribution in chinese cabbage (brassica rapa ssp. pekinensis). | this study investigated optimum light conditions for enhancing phenylpropanoid biosynthesis and the distribution of phenylpropanoids in organs of chinese cabbage (brassica rapa ssp. pekinensis). blue light caused a high accumulation of most phenolic compounds, including p-hydroxybenzoic acid, ferulic acid, quercetin, and kaempferol, at 12 days after irradiation (dai). this increase was coincident with a noticeable increase in expression levels of brf3h, brf3'h, brfls, and brdfr. red light led to ... | 2015 | 26158208 |
| genome-wide survey and expression profile analysis of the mitogen-activated protein kinase (mapk) gene family in brassica rapa. | mitogen-activated protein kinase (mapk) cascades are fundamental signal transduction modules in plants, controlling cell division, development, hormone signaling, and biotic and abiotic stress responses. although mapks have been investigated in several plant species, a comprehensive analysis of the mapk gene family has hitherto not been performed in brassica rapa. in this study, we identified 32 mapks in the b. rapa genome by conducting blastp and syntenic block analyses, and screening for the e ... | 2015 | 26173020 |
| characterization of fae1 in the zero erucic acid germplasm of brassica rapa l. | the modification of erucic acid content in seeds is one of the major goals for quality breeding in oil-yielding brassica species. however, few low erucic acid (lea) resources are available, and novel lea genetic resources are being sought. fatty acid elongase 1 (fae1) is the key gene that controls erucic acid synthesis. however, the mechanism for erucic acid synthesis in b. rapa lacks systematic study. here, we isolated zero erucic acid lines from 1981 chinese landraces of b. rapa and found that ... | 2015 | 26175623 |
| three genes encoding aop2, a protein involved in aliphatic glucosinolate biosynthesis, are differentially expressed in brassica rapa. | the glucosinolate biosynthetic gene aop2 encodes an enzyme that plays a crucial role in catalysing the conversion of beneficial glucosinolates into anti-nutritional ones. in brassica rapa, three copies of braop2 have been identified, but their function in establishing the glucosinolate content of b. rapa is poorly understood. here, we used phylogenetic and gene structure analyses to show that braop2 proteins have evolved via a duplication process retaining two highly conserved domains at the n-t ... | 2015 | 26188204 |
| the influence of various biochars on the bioaccessibility and bioaccumulation of pahs and potentially toxic elements to turnips (brassica rapa l.). | the influence of amending a contaminated soil with different dry-pyrolyzed biochars on the bioaccessibility and biouptake of polycyclic aromatic hydrocarbons (pah) and potentially toxic elements (pte) in turnip (brassica rapa l.,) was investigated. this is the first study to examine the influence of biochar amendments on turnips grown in a contaminated soil. the biochars came from different local feedstocks, including sewage sludge biochar (ssbc), soybean straw biochar (sbbc), rice straw biochar ... | 2015 | 26188867 |
| aboveground and belowground herbivores synergistically induce volatile organic sulfur compound emissions from shoots but not from roots. | studies on aboveground (ag) plant organs have shown that volatile organic compound (voc) emissions differ between simultaneous attack by herbivores and single herbivore attack. there is growing evidence that interactive effects of simultaneous herbivory also occur across the root-shoot interface. in our study, brassica rapa roots were infested with root fly larvae (delia radicum) and the shoots infested with pieris brassicae, either singly or simultaneously, to study these root-shoot interaction ... | 2015 | 26195194 |
| construction of brassica a and c genome-based ordered pan-transcriptomes for use in rapeseed genomic research. | this data article reports the establishment of the first pan-transcriptome resources for the brassica a and c genomes. these were developed using existing coding dna sequence (cds) gene models from the now-published brassica oleracea to1000 and brassica napus darmor-bzh genome sequence assemblies representing the chromosomes of these species, along with preliminary cds models from an updated brassica rapa chiifu genome sequence assembly. the b. rapa genome sequence scaffolds required splitting a ... | 2015 | 26217816 |
| leaf and root glucosinolate profiles of chinese cabbage (brassica rapa ssp. pekinensis) as a systemic response to methyl jasmonate and salicylic acid elicitation. | glucosinolates (gss) are an important group of defensive phytochemicals mainly found in brassicaceae. plant hormones jasmonic acid (ja) and salicylic acid (sa) are major regulators of plant response to pathogen attack. however, there is little information about the interactive effect of both elicitors on inducing gs biosynthesis in chinese cabbage (brassica rapa ssp. pekinensis). in this study, we applied different concentrations of methyl jasmonate (meja) and/or sa onto the leaf and root of chi ... | 2015 | 26238545 |
| tuning growth cycles of brassica crops via natural antisense transcripts of brflc. | several oilseed and vegetable crops of brassica are biennials that require a prolonged winter cold for flowering, a process called vernalization. flowering locus c (flc) is a central repressor of flowering. here, we report that the overexpression of natural antisense transcripts (nats) of brassica rapa flc (brflc) greatly shortens plant growth cycles. in rapid-, medium- and slow-cycling crop types, there are four copies of the brflc genes, which show extensive variation in sequences and expressi ... | 2016 | 26250982 |
| stress-responsive expression patterns and functional characterization of cold shock domain proteins in cabbage (brassica rapa) under abiotic stress conditions. | although the functional roles of cold shock domain proteins (csdps) have been demonstrated during the growth, development, and stress adaptation of arabidopsis (arabidopsis thaliana), rice (oryza sativa), and wheat (triticum aestivum), the functions of csdps in other plants species, including cabbage (brassica rapa), are largely unknown. to gain insight into the roles of csdps in cabbage under stress conditions, the genes encoding csdps in cabbage were isolated, and the functional roles of csdps ... | 2015 | 26263516 |
| conservation of trna and rrna 5-methylcytosine in the kingdom plantae. | post-transcriptional methylation of rna cytosine residues to 5-methylcytosine (m(5)c) is an important modification that regulates rna metabolism and occurs in both eukaryotes and prokaryotes. yet, to date, no transcriptome-wide identification of m(5)c sites has been undertaken in plants. plants provide a unique comparative system for investigating the origin and evolution of m(5)c as they contain three different genomes, the nucleus, mitochondria and chloroplast. here we use bisulfite conversion ... | 2015 | 26268215 |
| sequence and expression variation in suppressor of overexpression of constans 1 (soc1): homeolog evolution in indian brassicas. | whole genome sequence analyses allow unravelling such evolutionary consequences of meso-triplication event in brassicaceae (∼14-20 million years ago (mya)) as differential gene fractionation and diversification in homeologous sub-genomes. this study presents a simple gene-centric approach involving microsynteny and natural genetic variation analysis for understanding suppressor of overexpression of constans 1 (soc1) homeolog evolution in brassica. analysis of microsynteny in brassica rapa homeol ... | 2015 | 26276216 |
| a multi-layered screening method to identify plant regulatory genes. | we used a seven-step process to identify genes involved in glucosinolate biosynthesis and metabolism in the chinese cabbage (brassica rapa). we constructed an annotated data set with 34,570 unigenes from b. rapa and predicted 11,526 glucosinolate-related candidate genes using expression profiles generated across nine stages of development on a 47k-gene microarray. using our multi-layered screening method, we screened 392 transcription factors, 843 pathway genes, and 4,162 ortholog genes associat ... | 2016 | 26355777 |
| identification, evolution, and expression partitioning of mirnas in allopolyploid brassica napus. | the recently published genome of brassica napus offers for the first time the opportunity to gain insights into the genomic organization and the evolution of mirnas in oilseed rape. in this study, 12 small rna libraries from two b. napus cultivars (tapidor and ningyou7) and their four double-haploid lines were sequenced, employing the newly sequenced b. napus genome, together with genomes of its progenitors brassica rapa and brassica oleracea. a total of 645 mirnas including 280 conserved and 36 ... | 2015 | 26357884 |
| herbaspirillum sp. strain gw103 alleviates salt stress in brassica rapa l. ssp. pekinensis. | mutual interactions between plant and rhizosphere bacteria facilitate plant growth and reduce risks of biotic and abiotic stresses. the present study demonstrates alleviation of salt stress in brassica rapa l. ssp. perkinensis (chinese cabbage) by herbaspirillum sp. strain gw103 isolated from rhizosphere soil of phragmites australis. the strain was capable of producing plant beneficial factors, such as auxin, siderophore, and 1-aminocylopropane-1-carboxylic acid deaminase. treatment of strain gw ... | 2016 | 26358119 |
| integrative analysis of mrna and mirna expression profiles of the tuberous root development at seedling stages in turnips. | the tuberous root of brassica rapa l. (turnip) is an important modified organ for nutrition storage. a better understanding of the molecular mechanisms involved in the process of tuberous root development is of great value in both economic and biological context. in this study, we analyzed the expression profiles of both mrnas and mirnas in tuberous roots at an early stage before cortex splitting (es), cortex splitting stage (css), and secondary root thickening stage (rts) in turnip based on hig ... | 2015 | 26367742 |
| two cytochromes p450 catalyze s-heterocyclizations in cabbage phytoalexin biosynthesis. | phytoalexins are abundant in edible crucifers and have important biological activities, yet no dedicated gene for their biosynthesis is known. here, we report two new cytochromes p450 from brassica rapa (chinese cabbage) that catalyze unprecedented s-heterocyclizations in cyclobrassinin and spirobrassinin biosynthesis. our results provide genetic and biochemical insights into the biosynthesis of a prominent pair of dietary metabolites and have implications for pathway discovery across >20 recent ... | 2015 | 26389737 |
| heritability of floral volatiles and pleiotropic responses to artificial selection in brassica rapa. | the evolution of the vast diversity of floral volatiles is little understood, although they serve fundamental functions, such as pollinator attraction and herbivore deterrence. floral volatiles are often species specific, yet highly variable and sensitive to environmental factors. to date, nothing is known about the heritability of floral volatiles, and whether individual compounds can evolve independently or solely in concert with the whole volatile bouquet. we conducted bi-directional artifici ... | 2016 | 26391626 |
| [effect of genotype and medium culture content on microspore-derived embryo formation in chinese cabbage (brassica rapa ssp. chinensis) cv. lastochka]. | the influence of different factors on microspore embryogenesis in chinese cabbage (b. rapa ssp. chinensis) was studied. a genotype dependence for embryo formation was observed. the majority of embryos and plants were obtained from microspores isolated from flower buds (2-2.9 mm in length) and cultured in the nln liquid medium with 13% sucrose (w/v) supplemented with 24-epibrassinolide and 1% activated charcoal. embryos cultured on the 1/2 murashige-skoog culture medium with 2% sucrose (w/v), 0.1 ... | 2016 | 26415277 |
| comprehensive analysis of the flowering genes in chinese cabbage and examination of evolutionary pattern of co-like genes in plant kingdom. | in plants, flowering is the most important transition from vegetative to reproductive growth. the flowering patterns of monocots and eudicots are distinctly different, but few studies have described the evolutionary patterns of the flowering genes in them. in this study, we analysed the evolutionary pattern, duplication and expression level of these genes. the main results were as follows: (i) characterization of flowering genes in monocots and eudicots, including the identification of family-sp ... | 2015 | 26416765 |
| gdsl esterase/lipase genes in brassica rapa l.: genome-wide identification and expression analysis. | gdsl esterase/lipase proteins (gelps), a very large subfamily of lipolytic enzymes, have been identified in microbes and many plants, but only a few have been characterized with respect to their roles in growth, development, and stress responses. in brassica crops, as in many other species, genome-wide systematic analysis and functional studies of these genes are still lacking. as a first step to study their function in b. rapa ssp. pekinensis (chinese cabbage), we comprehensively identified all ... | 2016 | 26423069 |
| generation and characterization of brassica rapa ssp. pekinensis - b. oleracea var. capitata monosomic and disomic alien addition lines. | five monosomic alien addition lines (maals) of brassica rapa ssp. pekinensis - b. oleracea var. capitata were obtained by hybridization and backcrossing between b. rapa ssp. pekinensis (female parent) and b. oleracea var. capitata. the alien linkage groups were identified using 42 b. oleracea var. capitata linkage group-specific markers as b. oleracea linkage groups c2, c3, c6, c7 and c8. based on the chromosomal karyotype of root tip cells, these five maals added individual chromosomes from b. ... | 2015 | 26440082 |
| how different genetically manipulated brassica genotypes affect life table parameters of plutella xylostella (lepidoptera: plutellidae). | the fitness of plutella xylostella l. on different genetically manipulated brassica plants, including canola's progenitor (brassica rapa l.), two cultivated canola cultivars (opera and rgs003), one hybrid (hyula401), one gamma-ray mutant-rgs003, and one transgenic (pf) genotype was compared using two-sex and female-based life table parameters. all experiments were conducted in a growth chamber at 25±1°c, 65±5% relative humidity, and a photoperiod of 16:8 (l:d) h. there were significant differenc ... | 2015 | 26470162 |
| management of yellowmargined leaf beetle microtheca ochroloma (coleoptera: chrysomelidae) using turnip as a trap crop. | the yellowmargined leaf beetle, microtheca ochroloma stål, is a major pest of cruciferous vegetable crops in organic production systems. very few organically acceptable management options are currently available for this pest. field studies were conducted at a research station in alabama and at a commercial organic vegetable farm in florida to investigate the effectiveness of turnip, brassica rapa rapa, as a trap crop for m. ochroloma. in the research station trial with cabbage planted as the ca ... | 2015 | 26470380 |
| the high-throughput sequencing of small rnas profiling in wide hybridisation and allopolyploidisation between brassica rapa and brassica nigra. | small rnas play an important role in maintaining the genome reconstruction and stability in the plant. however, little is known regarding the role of small rnas during the process of wide hybridisation and chromosome doubling. therefore, the changes in the small rnas were assessed during the formation of an allodiploid (genome: ab) and its allotetraploid (genome: aabb) between brassica rapa (♀) and brassica nigra (♂) in the present study. here, the experimental methods described in details, rna- ... | 2015 | 26484138 |
| glucosinolate accumulation and related gene expression in pak choi (brassica rapa l. ssp. chinensis var. communis [n. tsen & s.h. lee] hanelt) in response to insecticide application. | glucosinolates and their breakdown products are well-known for their cancer-chemoprotective functions and biocidal activities against pathogens and generalist herbivores. insecticides are commonly used in the production of pak choi (brassica rapa l. ssp. chinensis var. communis [n. tsen & s.h. lee] hanelt). we studied the effects of four commonly used insecticides, namely, β-cypermethrin, acephate, pymetrozine, and imidacloprid, on glucosinolate metabolism in pak choi. all insecticides significa ... | 2015 | 26485123 |
| optimization of extraction, characterization and antioxidant activity of polysaccharides from brassica rapa l. | the root of brassica rapa l. has been traditionally used as a uyghur folk medicine to cure cough and asthma by uyghur nationality in xinjiang uygur autonomous region of china. in the present study, therefore, extraction optimization, characterization and antioxidant activity in vitro of polysaccharides from the root of b. rapa l. (brp) were investigated. the optimal extraction conditions with an extraction yield of 21.48 ± 0.41% for crude brp were obtained as follows: extraction temperature 93°c ... | 2016 | 26499088 |
| genome-wide dna methylation profiling by modified reduced representation bisulfite sequencing in brassica rapa suggests that epigenetic modifications play a key role in polyploid genome evolution. | brassica rapa includes some of the most important vegetables worldwide as well as oilseed crops. the complete annotated genome sequence confirmed its paleohexaploid origins and provides opportunities for exploring the detailed process of polyploid genome evolution. we generated a genome-wide dna methylation profile for b. rapa using a modified reduced representation bisulfite sequencing (rrbs) method. this sampling represented 2.24% of all cg loci (2.5 × 10(5)), 2.16% chg (2.7 × 10(5)), and 1.68 ... | 2015 | 26500672 |
| genome triplication drove the diversification of brassica plants. | the genus brassica belongs to the plant family brassicaceae, which includes many important crop species that are used as oilseed, condiments, or vegetables throughout the world. brassica plants comprise many diverse species, and each species contains rich morphotypes showing extreme traits. brassica species experienced an extra whole genome triplication (wgt) event compared with the model plant arabidopsis thaliana. whole genome sequencing of the brassica species brassica rapa, brassica oleracea ... | 2014 | 26504539 |
| characterization and development of est-ssrs by deep transcriptome sequencing in chinese cabbage (brassica rapa l. ssp. pekinensis). | simple sequence repeats (ssrs) are among the most important markers for population analysis and have been widely used in plant genetic mapping and molecular breeding. expressed sequence tag-ssr (est-ssr) markers, located in the coding regions, are potentially more efficient for qtl mapping, gene targeting, and marker-assisted breeding. in this study, we investigated 51,694 nonredundant unigenes, assembled from clean reads from deep transcriptome sequencing with a solexa/illumina platform, for id ... | 2015 | 26504770 |
| genome-wide identification, classification, and expression analysis of shsp genes in chinese cabbage (brassica rapa ssp pekinensis). | small heat shock proteins (shsps) are essential for the plant's normal development and stress responses, especially the heat stress response. the information regarding shsp genes in chinese cabbage (brassica rapa ssp pekinensis) is sparse, hence we performed a genome-wide analysis to identify shsp genes in this species. we identified 26 non-redundant shsp genes distributed on all chromosomes, except chromosome a7, with one additional shsp gene identified from an expressed sequence tag library. c ... | 2015 | 26505345 |
| dissecting the complex molecular evolution and expression of polygalacturonase gene family in brassica rapa ssp. chinensis. | polygalacturonases (pgs) participate in pectin disassembly of cell wall and belong to one of the largest hydrolase families in plants. in this study, we identified 99 pg genes in brassica rapa. comprehensive analysis of phylogeny, gene structures, physico-chemical properties and coding sequence evolution demonstrated that plant pgs should be classified into seven divergent clades and each clade's members had specific sequence and structure characteristics, and/or were under specific selection pr ... | 2015 | 26506823 |
| a systems genetics approach identifies gene regulatory networks associated with fatty acid composition in brassica rapa seed. | fatty acids in seeds affect seed germination and seedling vigor, and fatty acid composition determines the quality of seed oil. in this study, quantitative trait locus (qtl) mapping of fatty acid and transcript abundance was integrated with gene network analysis to unravel the genetic regulation of seed fatty acid composition in a brassica rapa doubled haploid population from a cross between a yellow sarson oil type and a black-seeded pak choi. the distribution of major qtls for fatty acids show ... | 2016 | 26518343 |
| genetic variation and inheritance of phytosterol and oil content in a doubled haploid population derived from the winter oilseed rape sansibar × oase cross. | identification of qtl for phytosterol content, oil content, fatty acids content, protein content of defatted meal, and seed weight by multiple interval mapping in a brassica napus dh population. phytosterols are minor seed constituents in oilseed rape which have recently drawn wide-interest from the food and nutrition industry due to their health benefit in lowering ldl cholesterol in humans. to understand the genetic basis of phytosterol content and its relationship with other seed quality trai ... | 2016 | 26518571 |
| user guidelines for the brassica database: brad. | the genome sequence of brassica rapa was first released in 2011. since then, further brassica genomes have been sequenced or are undergoing sequencing. it is therefore necessary to develop tools that help users to mine information from genomic data efficiently. this will greatly aid scientific exploration and breeding application, especially for those with low levels of bioinformatic training. therefore, the brassica database (brad) was built to collect, integrate, illustrate, and visualize bras ... | 2016 | 26519408 |
| impacts of whole-genome triplication on mirna evolution in brassica rapa. | micrornas (mirnas) are a class of short non-coding, endogenous rnas that play essential roles in eukaryotes. although the influence of whole-genome triplication (wgt) on protein-coding genes has been well documented in brassica rapa, little is known about its impacts on mirnas. in this study, through generating a comprehensive annotation of 680 mirnas for b. rapa, we analyzed the evolutionary characteristics of these mirnas from different aspects in b. rapa. first, while mirnas and genes show si ... | 2015 | 26527651 |
| ecological risk assessment of a metal-contaminated area in the tropics. tier ii: detailed assessment. | this study presents data on the detailed evaluation (tier 2) of a site-specific ecological risk assessment (ssera) in a former smelter area contaminated with metals (santo amaro, bahia, brazil). combining information from three lines of evidence (loe), chemical (chemloe), ecotoxicological (ecotoxloe) and ecological (ecoloe), in the triad approach, integrated risk values were calculated to rank sites and confirm the potential risk disclosed with tier 1. risk values were calculated for the habitat ... | 2015 | 26528915 |
| genome-wide analysis and expression patterns of zf-hd transcription factors under different developmental tissues and abiotic stresses in chinese cabbage. | the zf-hd gene family plays an important role in plant developmental processes and stress responses. however, the function of the zf-hd genes in chinese cabbage remains largely unknown. chinese cabbage (brassica rapa ssp. pekinensis) is a member of one of the most important leaf vegetables grown worldwide. the entire chinese cabbage genome sequence has been determined, and more than forty thousand proteins have been identified to date. in this study, 31 zf-hd genes were identified in chinese cab ... | 2016 | 26546019 |
| molecular evolution, characterization, and expression analysis of snrk2 gene family in pak-choi (brassica rapa ssp. chinensis). | the sucrose non-fermenting 1-related protein kinase 2 (snrk2) family members are plant-specific serine/threonine kinases that are involved in the plant response to abiotic stress and abscisic acid (aba)-dependent plant development. further understanding of the evolutionary history and expression characteristics of these genes will help to elucidate the mechanisms of the stress tolerance in pak-choi, an important green leafy vegetable in china. thus, we investigated the evolutionary patterns, foo ... | 2015 | 26557127 |
| the mirnas and their regulatory networks responsible for pollen abortion in ogura-cms chinese cabbage revealed by high-throughput sequencing of mirnas, degradomes, and transcriptomes. | chinese cabbage (brassica rapa ssp. pekinensis) is one of the most important vegetables in asia and is cultivated across the world. ogura-type cytoplasmic male sterility (ogura-cms) has been widely used in the hybrid breeding industry for chinese cabbage and many other cruciferous vegetables. although, the cause of ogura-cms has been localized to the orf138 locus in the mitochondrial genome, however, the mechanism by which nuclear genes respond to the mutation of the mitochondrial orf138 locus i ... | 2015 | 26557132 |
| atmospheric h2s and so2 as sulfur source for brassica juncea and brassica rapa: impact on the glucosinolate composition. | the impact of sulfate deprivation and atmospheric h2s and so2 nutrition on the content and composition of glucosinolates was studied in brassica juncea and b. rapa. both species contained a number of aliphatic, aromatic and indolic glucosinolates. the total glucosinolate content was more than 5.5-fold higher in b. juncea than in b. rapa, which could solely be attributed to the presence of high levels of sinigrin, which was absent in the latter species. sulfate deprivation resulted in a strong de ... | 2015 | 26579170 |
| genomic and post-translational modification analysis of leucine-rich-repeat receptor-like kinases in brassica rapa. | among several receptor-like kinases (rlks), leucine-rich-repeat receptor-like kinases (lrr-rlks) are a major group of genes that play crucial roles in growth, development and stress responses in plant systems. given that they have several functional roles, it is important to investigate their roles in brassica rapa. in the present study, 303 lrr-rlks were identified in the genome of b. rapa and comparative phylogenetic analysis of 1213 combined lrr-rlks of b. rapa, arabidopsis thaliana, oryza sa ... | 2015 | 26588465 |
| brassica database (brad) version 2.0: integrating and mining brassicaceae species genomic resources. | the brassica database (brad) was built initially to assist users apply brassica rapa and arabidopsis thaliana genomic data efficiently to their research. however, many brassicaceae genomes have been sequenced and released after its construction. these genomes are rich resources for comparative genomics, gene annotation and functional evolutionary studies of brassica crops. therefore, we have updated brad to version 2.0 (v2.0). in brad v2.0, 11 more brassicaceae genomes have been integrated into ... | 2015 | 26589635 |
| diversification and evolution of the sdg gene family in brassica rapa after the whole genome triplication. | histone lysine methylation, controlled by the set domain group (sdg) gene family, is part of the histone code that regulates chromatin function and epigenetic control of gene expression. analyzing the sdg gene family in brassica rapa for their gene structure, domain architecture, subcellular localization, rate of molecular evolution and gene expression pattern revealed common occurrences of subfunctionalization and neofunctionalization in brsdgs. in comparison with arabidopsis thaliana, the brsd ... | 2015 | 26596461 |
| the causes of selection on flowering time through male fitness in a hermaphroditic annual plant. | flowering is a key life-history event whose timing almost certainly affects both male and female fitness, but tests of selection on flowering time through male fitness are few. such selection may arise from direct effects of flowering time, and indirect effects through covariance between flowering time and the environment experienced during reproduction. to isolate these intrinsically correlated associations, we staggered planting dates of brassica rapa families with known flowering times, creat ... | 2016 | 26596860 |
| genome-wide analysis of the sbp-box gene family in chinese cabbage (brassica rapa subsp. pekinensis). | the squamosa promoter binding protein (sbp)-box gene family contains highly conserved plant-specific transcription factors that play an important role in plant development, especially in flowering. chinese cabbage (brassica rapa subsp. pekinensis) is a leafy vegetable grown worldwide and is used as a model crop for research in genome duplication. the present study aimed to characterize the sbp-box transcription factor genes in chinese cabbage. twenty-nine sbp-box genes were identified in the chi ... | 2015 | 26599708 |
| functional divergence of bak1 genes from brassica rapa in regulating plant architecture. | bak1 is a co-receptor of bri1 in early signaling pathways mediated by brassinosteroids (brs) and is thought to play a major role in plant growth and development. as the role of bak1 has not yet been fully elucidated then further research is required to explore its potential for use in genetic modification to improve crops. in this study, three bak1 genes from the amphidiploid species brassica rapa were isolated and their kinase functions were predicted following dna sequence analysis. a bioinfor ... | 2015 | 26600518 |
| analysis of genomic dna methylation and gene expression in chinese cabbage (brassica rapa l. ssp. pekinensis) after continuous seedling breeding. | vernalization plays a key role in the bolting and flowering of chinese cabbage (brassica rapa l. ssp. pekinensis). plants can switch from vegetative to reproductive growth and then bolt and flower under low temperature induction. the economic benefits of chinese cabbage will decline significantly when the bolting happens before the vegetative body fully grows due to a lack of the edible value. it was found that continuous seedling breeding reduced the heading of chinese cabbage and led to bolt a ... | 2015 | 26601490 |
| selection during crop diversification involves correlated evolution of the circadian clock and ecophysiological traits in brassica rapa. | crop selection often leads to dramatic morphological diversification, in which allocation to the harvestable component increases. shifts in allocation are predicted to impact (as well as rely on) physiological traits; yet, little is known about the evolution of gas exchange and related anatomical features during crop diversification. in brassica rapa, we tested for physiological differentiation among three crop morphotypes (leaf, turnip, and oilseed) and for correlated evolution of circadian, ga ... | 2016 | 26618783 |
| genotyping-by-sequencing map permits identification of clubroot resistance qtls and revision of the reference genome assembly in cabbage (brassica oleracea l.). | clubroot is a devastating disease caused by plasmodiophora brassicae and results in severe losses of yield and quality in brassica crops. many clubroot resistance genes and markers are available in brassica rapa but less is known in brassica oleracea. here, we applied the genotyping-by-sequencing (gbs) technique to construct a high-resolution genetic map and identify clubroot resistance (cr) genes. a total of 43,821 snps were identified using gbs data for two parental lines, one resistant and on ... | 2016 | 26622061 |
| genome-wide identification and analysis of the vq motif-containing protein family in chinese cabbage (brassica rapa l. ssp. pekinensis). | previous studies have showed that the vq motif-containing proteins in arabidopsis thaliana and oryza sativa play an important role in plant growth, development, and stress responses. however, little is known about the functions of the vq genes in brassica rapa (chinese cabbage). in this study, we performed genome-wide identification, characterization, and expression analysis of the vq genes in chinese cabbage, especially under adverse environment. we identified 57 vq genes and classified them in ... | 2015 | 26633387 |
| variation in the flowering time orthologs brflc and brsoc1 in a natural population of brassica rapa. | understanding the genetic basis of natural phenotypic variation is of great importance, particularly since selection can act on this variation to cause evolution. we examined expression and allelic variation in candidate flowering time loci in brassica rapa plants derived from a natural population and showing a broad range in the timing of first flowering. the loci of interest were orthologs of the arabidopsis genes flc and soc1 (brflc and brsoc1, respectively), which in arabidopsis play a centr ... | 2015 | 26644966 |
| increased susceptibility to fungal disease accompanies adaptation to drought in brassica rapa. | recent studies have demonstrated adaptive evolutionary responses to climate change, but little is known about how these responses may influence ecological interactions with other organisms, including natural enemies. we used a resurrection experiment in the greenhouse to examine the effect of evolutionary responses to drought on the susceptibility of brassica rapa plants to a fungal pathogen, alternaria brassicae. in agreement with previous studies in this population, we found an evolutionary sh ... | 2016 | 26648585 |
| quantitative trait locus analysis of seed germination and seedling vigor in brassica rapa reveals qtl hotspots and epistatic interactions. | the genetic basis of seed germination and seedling vigor is largely unknown in brassica species. we performed a study to evaluate the genetic basis of these important traits in a b. rapa doubled haploid population from a cross of a yellow-seeded oil-type yellow sarson and a black-seeded vegetable-type pak choi. we identified 26 qtl regions across all 10 linkage groups for traits related to seed weight, seed germination and seedling vigor under non-stress and salt stress conditions illustrating t ... | 2015 | 26648948 |
| understanding brassicaceae evolution through ancestral genome reconstruction. | brassicaceae is a family of green plants of high scientific and economic interest, including thale cress (arabidopsis thaliana), cruciferous vegetables (cabbages) and rapeseed. | 2015 | 26653025 |
| cerium oxide nanoparticles and bulk cerium oxide leading to different physiological and biochemical responses in brassica rapa. | cerium oxide nanoparticles (ceo2nps) have been incorporated into many commercial products, and their potential release into the environment through the use and disposal of these products has caused serious concerns. despite the previous efforts and rapid progress on elucidating the environmental impact of ceo2nps, the long-term impact of ceo2nps to plants, a key component of the ecosystem, is still not well understood. the potentially different impact of ceo2nps and their bulk counterparts to pl ... | 2016 | 26691446 |
| snp diversity within and among brassica rapa accessions reveals no geographic differentiation. | genetic diversity was studied in a collection of 61 accessions of brassica rapa, which were mostly oil-type turnip rapes but also included two oil-type subsp. dichotoma and five subsp. trilocularis accessions, as well as three leaf-type subspecies (subsp. japonica, pekinensis, and chinensis) and five turnip cultivars (subsp. rapa). two-hundred and nine snp markers, which had been discovered by amplicon resequencing, were used to genotype 893 plants from the b. rapa collection using illumina bead ... | 2016 | 26694015 |
| a transposon insertion in flowering locus t is associated with delayed flowering in brassica rapa. | long days and vernalization accelerate the transition from vegetative growth to reproductive growth in brassica rapa. bolting before plants reach the harvesting stage is a serious problem in b. rapa vegetable crop cultivation. the genetic dissection of flowering time is important for breeding of premature bolting-resistant b. rapa crops. using a recombinant inbred line (ril) population, we twice detected two major quantitative trait loci (qtls) for flowering time in two different growing seasons ... | 2015 | 26706072 |
| the complete chloroplast genome sequence of indian mustard (brassica juncea l.). | brassica juncea l. is the second most important edible oil seed crop of india. in the current study, we report the complete chloroplast genome sequence of indian mustard, brassica juncea l. the size of the complete chloroplast genome was found to be 153 483 bp long with an overall gc content of 36.36%. a large single copy (lsc) region of 83 286 bp and a short single copy region (ssc) region of 17 775 bp were separated by a pair of inverted repeat (ir) regions of 26 211 bp. a total of 113 unique ... | 2016 | 26708222 |
| anthocyanin accumulation, antioxidant ability and stability, and a transcriptional analysis of anthocyanin biosynthesis in purple heading chinese cabbage (brassica rapa l. ssp. pekinensis). | heading chinese cabbage (brassica rapa l. ssp. pekinensis) is a significant dietary vegetable for its edible heading leaves in asia countries. the new purple anthocyanin-rich pure line (11s91) was successfully bred, and the anthocyanins were mainly distributed in 2-3 cell layers beneath the leaf epidermis, whereas siliques and stems accumulated only a cell layer of anthocyanins. the anthocyanins of 11s91 were more stable at phs below 3.0 and temperatures below 45 °c. the total antioxidant abilit ... | 2016 | 26709726 |
| retention, molecular evolution, and expression divergence of the auxin/indole acetic acid and auxin response factor gene families in brassica rapa shed light on their evolution patterns in plants. | auxin/indole acetic acids (aux/iaas) and auxin response factors (arfs), major components of the aux signaling network, are involved in many developmental processes in plants. investigating their evolution will provide new sight on the relationship between the molecular evolution of these genes and the increasing morphotypes of plants. we constructed comparative analyses of the retention, structure, expansion, and expression patterns of aux/iaas and arfs in brassica rapa and their evolution in ei ... | 2015 | 26721260 |
| antioxidant capacities and polyphenolics of chinese cabbage (brassica rapa l. ssp. pekinensis) leaves. | chinese cabbage (brassica rapa l. ssp. pekinensis) is a green leafy vegetable used mainly in kimchi, salted and fermented dishes. consumer preference for the leaf portion differs according to the type of dishes. in this study, chinese cabbage was divided into three parts, and their antioxidant activities were investigated through in vitro assays. the total phenolic contents (tpc), total flavonoid contents (tfc), and vitamin c contents were also determined as indicators of antioxidant contents. t ... | 2016 | 26776015 |
| transcriptome analysis of brassica rapa near-isogenic lines carrying clubroot-resistant and -susceptible alleles in response to plasmodiophora brassicae during early infection. | although plasmodiophora brassicae is one of the most common pathogens worldwide, the causal agent of clubroot disease in brassica crops, resistance mechanisms to it are still only poorly understood. to study the early defense response induced by p. brassicae infection, a global transcriptome profiling of the roots of two near-isogenic lines (nils) of clubroot-resistant (cr bjn3-2) and clubroot-susceptible (bjn3-2) chinese cabbage (brassica rapa) was performed by rna-seq. among the 42,730 unique ... | 2015 | 26779217 |
| cloning and characterization of the nicotianamine synthase gene in eruca vesicaria subsp sativa. | nicotianamine (na) is a ubiquitous metabolite in plants that bind heavy metals, is crucial for metal homeostasis, and is also an important metal chelator that facilitates long-distance metal transport and sequestration. na synthesis is catalyzed by the enzyme nicotianamine synthase (nas). eruca vesicaria subsp sativa is highly tolerant to ni, pb, and zn. in this study, a gene encoding evnas was cloned and characterized in e. vesicaria subsp sativa. the full-length evnas cdna sequence contained a ... | 2015 | 26782459 |
| genetic architecture, biochemical underpinnings and ecological impact of floral uv patterning. | floral attraction traits can significantly affect pollinator visitation patterns, but adaptive evolution of these traits may be constrained by correlations with other traits. in some cases, molecular pathways contributing to floral attraction are well characterized, offering the opportunity to explore loci potentially underlying variation among individuals. here, we quantify the range of variation in floral uv patterning (i.e. uv 'bulls-eye nectar guides) among crop and wild accessions of brassi ... | 2016 | 26800256 |