experimental retinal branch vein occlusion in the monkey. histopathological and ultrastructural studies.light and electron microscopical studies of monkey retinae after experimental branch vein occlusion by argon laser reveal that the immediate reaction in the corresponding region is one of capillary engorgement and dilatation followed by oedema (intra- and extracellular), and plasma leakage as shown by intravenous injection of horseradish peroxidase. vascular stasis is characterized by packing of erythrocytes in the capillary lumen, early thrombus formation, and focal haemorrhages. progressive de ...1976827828
is there a blood-brain barrier at the optic nerve head?the retina and optic nerve have been demonstrated to possess a blood-brain barrier that prevents the passive passage of protein and certain dyes from the blood vessels into the extracellular space. our observations suggest that an exception is present at the normal optic disc. using horseradish peroxidase as a tracer for electron microscopy and the normal rhesus monkey as the experimental animal, we have demonstrated that in certain regions of the optic nerve head, horseradish peroxidase from th ...1975828849
afferent projections to the cat locus coeruleus as visualized by the horseradish peroxidase technique. 1977830382
serotonergic afferents to the dorsal raphe nucleus: evdience from hrp and synaptosomal uptake studies.afferent connections of the serotonin (5-ht)-containing dorsal raphe nucleus were investigated in the rat utilizing the horseradish peroxidase (hrp) retrograde cell labeling technique. small quantities (0.1-0.5 mul) of hrp solutions were infused into the dorsal raphe, and the brains were examined 19-72 h later for retrograde transport of the enzyme. intrinsic connections within the dorsal raphe nucleus were revealed by this mapping technique, as was an input to the dorsal raphe from another sero ...1977830388
pseudopodia formation by neurosecretory granules.ultrastrucal studies of the mouse neurohypophysis, under various experimental conditions, revealed a number of neurosecretory granules (nsg) bearing single pseudopodia-like protrusions. some nsg adhered to the axolemma via pseudopodia; other nsg, distant from the axolemma, budded electron lucent microvesicles from the tip of the pseudopod. pseudopodia counts were made on electron micrographs, and calculated as a percentage of the nsg population. in neural lobes from intact mice, small numbers of ...1977830428
amygdala afferents from the mediobasal hypothalamus: an electrophysiological and neuroanatomical study in the rat.electrophysiological techniques and the retrograde transport of horseradish peroxidase (hrp) were used to determine the efferent projections from the caudal mediobasal hypothalamus to the amygdala. in pentobarbital anesthetized rats, the activity of 1780 mediobasal hypothalamic neurons was examined for response to stimulation sites in the amygdala and stria terminalis. evidence of orthodromic activation from both stimulation sties was commonly observed. sixty-five cells mostly located in the ven ...1977832160
protamine induced intracellular uptake of horseradish peroxidase and vacuolation in mouse skeletal muscle in vitro.the uptake in vitro of horseradish peroxidase (hrp) in mouse skeletal muscle was examined by electron microscopy and chemical determination. in muscles exposed to an hrp solution for 60 min at +37 degrees c, hrp infiltrated the basal lamina of muscle fibres and caused an intense labelling of their sarcolemma. in addition hrp was found within the transverse tubules. exposure to hrp for 30 min at +37 degrees c followed by hrp together with a polycationic protein (protamine) for 30 min at +37 degre ...1977832308
effect of pentobarbital on uptake of horseradish peroxidase by rabbit cortical synapses. 1977832696
sequential uptake of horseradish peroxidase by lymphoid follicle epithelium of peyer's patches in the normal unobstructed mouse intestine: an ultrastructural study.membranous epithelial or "m" cells in lymphoid follicle epithelium of peyer's patches extend between the adjacent columnar cells, forming a membrane separating lymphocytes in the epithelial cell layer from the intestinal lumen. they lack developed microvilli, glycocalyx, or terminal web but contain numerous vesicles. transport of particles from ligated intestinal loops by such cells without evidence of further uptake of these particles by lymphocytes has been described by others. horseradish per ...1977832793
pulmonary capillary permeability to hrp in dogs: a physiological and morphological study.pulmonary capillary permeability was studied in 10 normal anesthetized dogs using horseradish peroxidase (hrp) as tracer. physiological studies involved measurement of left ventricular, pulmonary arterial, and wedge pressures, and of right lymphatic duct (rld) and thoracic duct lymph flows. plasma and interstitial oncotic pressures were calculated from protein measurements. after 1 h of lymph collection, hrp was injected intravenously at three total dose levels, low (20-30 mg/kg), medium (55 mg/ ...1977833071
immunoelectronmicroscopic localization of alpha-actinin in skeletal muscle cells.the ultrastructural distribution of alpha-actinin in skeletal myocytes was studied by a sensitive method that permits localization of antigenic proteins in intact fixed cells rather than in glycerol estracted tissues or isolated cell fractions. binding to the z line of specific antiserum produced in rabbits immunized with highly purified alpha-actinin was demonstrated by deposition at this site of a reaction product of horseradish peroxidase that had been coupled to antigen-binding fragments of ...1977833423
concanavalin a receptors on the surfaces of human breast cancer cells in organ culture.the binding of concanavalin a (con a) at the free apical membranes of surface tumor cells in human breast cancer explants grown in organ culture was studied cytochemically with horseradish peroxidase (hrp) as a marker. on the cell membranes in aldehyde-fixed explants or explants exposed to con a at 4 degrees c, a continuous label covered the entire free surface of the cell, which indicated the dispersed distribution of con a binding sites. the binding of con a at 20 degrees c resulted in discont ...1977833861
amygdaloid projections to prefrontal and motor projections from the amygdala to the entire frontal cortex were demonstrated in the cat using the retrograde transport of horseradish peroxidase. injections throughout the prefrontal cortex labeled neurons in the ipsilateral basal magnocellular amygdaloid nucleus; injections in the premotor and motor cortices labeled neurons in the same nucleus plus a few cells in the anterior amygdaloid area.1977836591
evidence of the dual innervation of the cat stomach by the vagal dorsal motor and medial solitary nuclei as demonstrated by the horseradish peroxidase method. 1977837215
electrophysiological and horseradish peroxidase studies of precerebellar afferents to the nucleus interpositus anterior. i. climbing fiber system.the input to the nucleus interpositus anterior (nia) of the cat from the inferior olive (io) was studied by stimulating the io while recording intracellularly from the nia, and by the retrograde transport of horseradish peroxidase (hrp). stimulation of the io evoked monosynaptic epsps in nia neurons. the cells labeled in the io following electrophoretic and pressure injection of hrp into nia were located in the rostral parts of the dorsal and medial accessory olive. stimulation of the io also po ...1977837228
electrophysiological and horseradish peroxidase studies of precerebellar afferents to the nucleus interpositus anterior. ii. mossy fiber system.inputs to the nucleus interpositus anterior (nia) of the cat from precerebellar nuclei which are thought to give rise to mossy fibers were studied using electrophysiological and anatomical techniques. stimulation of one of these precerebellar nuclei, the lateral reticular nucleus (lrn) evoked monosynaptic epsps in nia neurons. these epsps were followed by polysynaptic ipsps and late depolarization mediated by the response of the cerebellar cortex. similar responses were occasionally seen followi ...1977837229
habenular and other midbrain raphe afferents demonstrated by a modified retrograde tracing technique.afferents to th midbrain dorsal and median raphe nuclei in the rat were studied by means of the horseradish peroxidase (hrp) retrograde transport method. the hrp was given by means of a modified iontophoretic delivery technique. this technique permitted an efficient and localized deposition of a high concentration of hrp into the raphe nuclei. afferents to the raphe as determined by this method could be categorized into 2 classes; those exclusively to the raphe and those also positive for adjace ...1977837230
central connections of ventral root afferents as demonstrated by the hrp study the central connections of ventral root afferents, horseradish peroxidase was injected into the lumbosacral spinal cord of the cat and the appropriate dorsal root ganglia were examined from segments with (1) both dorsal and ventral roots intact, (2) both roots sectioned, and (3) only the dorsal root sectioned. the key finding was that a number of labeled cells (up to 133) were observed in the ganglion after dorsal rhizotomy. we interpret these findings to mean that the central processes ...1977838891
altered renal cortical reabsorption of protein and urinary excretion of sodium in relation to vascular leakage induced by horseradish peroxidase.the intravenous injection of horseradish peroxidase (hrp) into rats of the sprague-dawley strain caused vascular leakage as detectable by a 20-40% increase in the hematocrit and a 15-20% decrease in plasma protein concentration. these changes did not occur when the same amounts of hrp were injected into rats pretreated with antagonists to histamine and serotonin. after pretreatment with the antagonists, the reabsorption of hrp by the proximal tubule cells (the concentration of hrp in the total p ...1977839063
the distribution of peroxidases in the sciatic nerves of normal and hexachlorophene intoxicated developing rats.horseradish peroxidase (mol. wt. 40 000) or microperoxidase (mol. wt. 1900) were injected over the sciatic nerve of normal or hexachlorophene (hcp) intoxicated developing rats (3,7,14 and 21 days). light and electron microscopic studies of nerves after histochemical staining for peroxidase revealed: a) the perineurial barrier to the two peroxidases was established in 21 day normal and hcp intoxicated rats; b) in animals 3-14 days, the perineurial barrier to both peroxidases was not formed and pe ...1977839249
cerebral cortical changes in acute experimental hypertension: an ultrastructural study.multiple focal cortical areas of increased vascular permeability to tracer substances occur in experimental hypertensive encephalopathy. in this study, rats with angiotensin-induced acute hypertension were used to determine whether increased permeability was associated with focal cerebral edema and if so, the tissue component involved. in addition, the mechanism of increased permeability and the types of vessels involved were investigated using horseradish peroxidase as a tracer. quantitative mo ...1977839731
experimental immunologic thrombocytopenia in dogs: a study of thrombocytopenia and megakaryocytopoiesis.immunologic thrombocytopenia was induced in dogs by intravenous or intraperitoneal injection of rabbit anti-canine platelet serum (aps) or by intraperitoneal injection of the igg fraction of the aps. in contrast, dogs injected with normal rabbit serum (nrs) or the igm fraction of the aps did not develop thrombocytopenia. marrow samples obtained at 24-96 h after inoculation of the igg fraction contained megakaryocytes giving a positive reaction with flurescein isothiocyanate or horseradish perox ...1977841193
cellular uptake of exogenous horseradish peroxidase in mouse peripheral nerve.horseradish peroxidase (hrp) was given to adult normal mice either intravenously or locally around the sciatic nerve. after varying time intervals the animals were sacrificed and fixed by whole body perfusion. the sciatic nerve was sampled and the distribution of peroxidatic activity was studied by light and electron microscopy. after both types of hrp administration the tracer was rapidly taken up by epi- and endoneurial cells with the ultrastructural features of macrophages. when increasing do ...1977842294
stability of the intra-epithelial component of the blood-testis barrier in epinephrine-induced testicular degeneration in syrian male syrian hamsters were given daily intraperitoneal injections of epinephrine (1.0 mg/kg) and papaverine, a vasodilator, (60 mg/kg) for a period of ten days. after the treatment period, lanthanum and horseradish peroxidase tracer studies were used to examine the intra-epithelial component of the blood-testis barrier. degenerating tubules often exhibited only sertoli cells and spermatogonia, or sertoli cells alone. sertoli cell processes in the degenerating tubules often arched out from t ...1977842474
the pontine projection to the cerebellar vermal visual area studied by means of the retrograde axonal transport of horseradish peroxidase.following injections of horseradish peroxidase (hrp) in cerbellar vermal lobules vi, viia and b, viia and b in the cat, the distribution of labeled cells in the pontine nuclei was mapped in drawings of serial transverse and horizontal sections. the labeled pontine cells are distributed in 4 largely longitudinal columns, situated in the dorsolateral, peduncular, lateral and paramedian pontine nucleus (referred to as columns a, b, c and d, respectively). the majority of afferents to the vermal, vi ...1977843923
vestibular and medullary brain stem afferents to the abducens nucleus in the cat.brain stem neurons that project to the abducens nucleus (nvi) were labeled by the technique of retrograde transport of horseradish peroxidase (hrp). following iontophoresis of hrp into nvi a large number of labeled cells are found in the ipsilateral vestibular nuclear complex, extending from the rostral medial vestibular nucleus into the ventral lateral vestibular nucleus. a smaller number of hrp-positive cells are also found in the contralateral medial vestibular nucleus. in addition, labeled c ...1977843924
the uptake of horseradish peroxidase by cortical synapses in rat brain. an in vivo study.horseradish peroxidase (hrp) was introduced directly into the cerebral cortex of adult rats, which were allowed to survive for 60 min before perfusion fixation. after the tissue had been incubated to demonstrate hrp at the lm and em levels, blocks of cortical tissue were taken at varying distances from the injection site. these eight blocks of tissue constituted a time sequence for hrp diffusion. qualitative examination of the presynaptic terminals showed that the most commonly encountered profi ...1977844083
afferent connections of the habenular nuclei in the rat. a horseradish peroxidase study, with a note on the fiber-of-passage problem.the afferent connections of the habenular complex in the rat were examined by injecting horseradish peroxidase (hrp) into discrete portions of the habenular nuclei by microelectrophoresis. 1. hrp deposits confined to the lateral half of the lateral habenular nucleus labeled a multitude of cells in the entopeduncular nucleus. numerous labeled cells also appeared in such cases in the lateral hypothalamus, indicating that the lateral habenular nucleus is a major convergence point of projections fro ...1977845280
the commissural projection of the superior colliculus in the cat.the origin, course, and termination of the commissural projection of the superior colliculus were studied using the orthograde and autoradiographic tracing method and the retrograde method utilizing horseradish peroxidase. the complementary and mutually confirming sets of data showed that the commissural fibers interconnect a restricted region of the colliculi. this region includes the strata grisea intermedium and profundum and to a lesser degree the stratum opticum. it extends throughout only ...1977845285
increased vesicular transfer of horseradish peroxidase across cerebral endothelium, evoked by acute hypertension.acute hypertension in rats was produced by intravenous infusion of metaraminol bitartrate (aramine). the permeability to intravenously injected horseradish peroxidase (hrp) was increased across the cerebral arterioles, capillaries and venules. from the basement membranes of the vessel walls the protein tracer moved into the extracellular spaces of the adjacent neuropil. no endothelial cell damage was observed. the tight junctions between endothelial cells were intact and prevented intercellular ...1977848278
attachment and ingestion of mycoplasmas by mouse macrophages. ii. scanning electron microscopic observations.mycoplasmas adhere closely to the central region of the surface of mouse peritoneal macrophages in vitro. they do not appear connected to each other or the macrophage membrane, and they induce no change in the surface of the cell. after addition of antimycoplasma antibody, mycoplasmas show interconnections and the cell shows an increase occurrence of ruffled membrane and folding over the mycoplasmas. large and small lacunae appear in the membrane at sites other than those taking in organisms, an ...1977851171
thalamic projections to parietal cortex.thalamic projections to parietal regions of cerebral cortex were investigated in the cat by retrograde degeneration and retrograde transport techniques. studies of retrograde cellular changes indicate cortically projecting cells in the lateroporterior nucleus (lp), rostral pulvinar (pul), and the ventral part of the laterodorsal nucleus (ld). when lesions were placed in the cortex of young kittens, clear chromatolytic changes and cell loss were also consistently observed in middle and caudal par ...1977851845
convergence of excitatory synaptic inputs to caudate spiny neurons.intracellular recordings were obtained from cat caudate nucleus neurons. these cells responded with convergent, monosynaptic epsps to cerebral cortex, intralaminar thalamus, and substantia nigra stimulation. intracellular injection of horseradish peroxidase into these electrophysiologically defined neurons shows that they represent the spiny neuron of the caudate based upon somadendritic morphology. the axons of these cells are appreciably longer than previous reports indicate. several lines of ...1977851861
permeability and patency of retinal blood vessels in experimental diabetes.increased permeability of retinal blood vessels in human diabetic retinopathy is well known clinically. its morphologic equivalent is unknown. in dogs with 5 years of poorly controlled alloxan diabetes and nonproliferative diabetic retinopathy comparable to that of man, permeability and patency of retinal blood vessels were tested with the protein tracer horseradish peroxidase and evaluated by electron microscopy. a breakdown of the blood-retinal barrier was found associated with extensive trace ...1977852945
internalization of lectins in neuronal gerl.conjugates of ricin agglutinin and phytohemagglutinin with horseradish peroxidase (hrp) were used for a cytochemical study of internalization of their plasma membrane "receptors" in cultured isolated mouse dorsal root ganglion neurons. labeling of cells with lectin-hrp was done at 4 degrees c, and internalization was performed at 37 degrees c in a culture medium free of lectin-hrp. 15-20 min after incubation at 37 degrees c, lectin-hrp receptor complexes were seen in vesicles or tubules located ...1977856827
anatomy of interhemispheric connections in the visual system of boston siamese and ordinary siamese cats, previous studies have shown that a genetic mutation causes retinogeniculate fibers in each eye which arise from the temporal retina representing the 20 degrees of ipsilateral visual field adjacent to the vertical meridian to cross aberrantly in the optic chiasm, thereby terminating in the wrong lateral geniculate nucleus. the abnormality is expressed subsequently at the level of the visual cortex. this paper presents anatomical evidence that the pattern of commissural visual con ...1977856894
the projection from the lateral geniculate nucleus onto the visual cortex in the cat. a quantitative study with horseradish-peroxidase.horseradish-peroxidase (hrp) was injected (9-18 microng in 0.03-0.06 micronl) into cortical areas 17, 18 or 19 of 11 adult cats. after survival times of 17 hours to 7 days, the thalamus was examined for retrogradely hrp labelled nerve cells in serial transverse sections. from these sections, the percentage of labelled cells occurring in each subdivision of the dorsal lateral geniculate nucleus (lgnd) was calculated for each animal. one case each for injections in areas 17, 18, and 19 was then ch ...1977856895
site of horseadish peroxidase transport by the small intestine of the neonatal rat.the absorption of horseradish peroxidase was estimated in 10-day-old rats by measuring plasma peroxidase levels following intraduodenal admininstration. peak levels occurred 45 min after injection. plasma peroxidase levels, 45 min after injection into five ligated segments along the small intestine, were significantly lower in rats with distal segments than in rats with proximal segments. this indicates that the proximal small intestine, including the duodenum, may play a significant role in non ...1977861316
origins of primary afferent fibers in the spinal ventral roots in the cat as demonstrated by the horseradish peroxidase method. 1977861724
ultrastructural characteristics of the brain and blood-brain barrier in experimental seizures.during experimental seizures, the blood-brain barrier (bbb) is broken; tracer substances such as i131-albumin, evans blue and horseradish peroxidase (hrp) geographically locate the barrier breakdown primarily in the diencephalon. using rats, we have induced seizures with electroshocks and demonstrated the breakdown of the bbb with evans blue and hrp. we have shown that (1) the bbb breakdown is proportional to the number of electroconvulsant shocks (es) given; (2) the mechanism of increased barri ...1977861758
control of density and microredistribution of concanavalin-a receptors in rat thymocytes at 4 degrees a previous paper, we reported that at 4 degrees c, a cooperative binding of concanavalin a to rat thymocytes is observed which corresponds to a modification of the membrane leading to the recruitment of receptors and their immobilization. in this paper, we report that both phenomena are modulated at 4 degrees c by drugs such as colchicine and cytochalasin b; cooperative binding of concanavalin a, which reflects receptor recruitment is only slightly modified by each drug alone. when the two dr ...1977862619
wafer embedding: specimen selction in electron microscopic cytochemistry with osmiophilic polymers.a new wafer embedding procedure is described that permits light microscopic screening of embedded tissue prior to ultrathin sectioning. it is particularly valuable when used on specimens obtained with an automatic sectioner and treated cytochemically to obtain visible intermediate or visible and electron opaque final reaction products. aldehyde-fixed tissues are cut into sections with an automatic sectioner, incubated cytochemically osmication if required, then embedded in epoxy resin between fl ...1977863747
experimental studies on cardiac muscle cell adaptation to insult.studies were carried out in rats on myocardial adaptation to injury produced by left coronary artery ligation. in the ischemic zone, open collaterals were present as shown by studies using the fine structural extracellular protein tracer, horseradish peroxidase (hrp). this phenomenon may explain the inhomogeneous cardiac muscle cell alteration in the early phase of coronarogenic myocardial injury. reperfusion, as evidenced by the influx of hrp into the damaged cells, unmasked sarcolemmal membran ...1977871298
reticulo-cuneate projections as revealed by horseradish peroxidase axonal transport. 1977871918
descending connections between superior olivary and cochlear nuclear complexes in the cat studied by autoradiographic and horseradish peroxidase methods.the descending pathways from the superior olivary complex (so) to the cochlear nuclear complex (cn) were investigated in 58 cats using labelled aminoacid and horseradish peroxidase transport techniques. descending connections were found coursing bilaterally through the trapezoid body (tb) and ipsilaterally in the intermediate and dorsal acoustic striae. the dorsolateral periolivary nucleus (dlpo) sends fibres through both the intermediate and dorsal striae, which are joined by others from the la ...1977880993
direct projections from thalamic intralaminar nuclei to extra-striate visual cortex in the cat traced with horseradish peroxidase.thalamic projections to the visual cortex were investigated using the horseradish peroxidase tracing technique. besides confirmation of a distinct origin of thalamic projections to striate and extra-striate visual cortex, afferents of the intralaminar nuclei (iln) to visual cortex were demonstrated. these projections of iln were shown to be specific in that they terminate in areas 18, 19 and clare bishop but not area 17. the coupling of these intralaminar projections on to the extra-striate visu ...1977880998
ultrastructural observations of the abducens nucleus of the cat after injection of horseradish peroxydase into the lateral rectus muscle.the ultrastructure of cat abducens motoneurones is described, after labelling by retrograde axonal transport of horseradish peroxydase. the motoneurones were densely covered with synaptic boutons of both the "terminal" and "en passant" types and axo-axonal synapses were observed on the initial segment of motoneurone axons. within the population of axons forming the vith nerve, multiple branching was encountered and axons closely apposed at the nodes of ranvier were seen.1977881006
locating corticospinal neurons by retrograde axonal transport of horseradish peroxidase. 1977885197
tectal projection of displaced ganglion cells in avian retina.labeled displaced ganglion cells (dgc's) were observed in chick embryos (day 12 to 18) and hatchings following discrete tectal injections of horseradish peroxidase (hrp). average dgc diameter was 10 to 15 mu, approximately twice the size of orthotopic ganglion cells. six to 12 dgc's were found per 15 mu section. these findings conclusively demonstrate a tectal projection for the dgc.1977885687
postnatal differentiation of cell body volumes of spinal motoneurons innervating slow-twitch and fast-twitch muscles.distribution of cell body volumes of motoneurons innervating the soleus (slow-twitch or tonic) and medial gastrocnemius (fast-twitch or phasic) muscles was examined in adult cats and a series of kittens ranging from one to 140 days in age. to identify the soleus (sol) and the medial gastrocnemius (mg) motoneurons, each group of motoneurons was labeled differentially by utilizing retrograde axonal transport of horseradish peroxidase injected into the muscles. it was verified statistically that in ...1977886025
projections from the inferior olive to the cerebellar nuclei in the cat demonstrated by retrograde transport of horseradish peroxidase.the method of intracerebral injections of horseradish peroxidase has been applied to demonstrate a projection from the inferior olivary nucleus to the intracerebellar nuclei in the cat. cells labeled by the transported enzyme can be observed in different regions of the olive according to the localization of the injection. the caudal half of the medial and dorsal accessory subdivisions, the dorsal cap and nucleus beta project to the fastigial nucleus. in the rostral half of the olive, the accesso ...1977890443
origins of vagal preganglionic fibers to the sino-atrial and atrio-ventricular node regions in the cat heart as studied by the horseradish peroxidase method. 1977890451
locus coeruleus-to-dorsal raphe input examined by electrophysiological and morphological order to examine the hypothesis that the locus coeruleus (lc) projects directly to the nucleus raphe dorsalis (dr), electrical stimulation was applied to the lc of rats while recording from single neurons in the region of the dr. slow firing units of the dr were not influenced by the stimulation, although faster firing units in the nearby substantia grisea centralis (sgc) were. these latter cells become oscillatory in their firing rates during lc stimulation. in parallel studies a retrograde ...1977890505
circular dichroism of turnip peroxidases.circular dichroic (cd) spectra of two turnip isoperoxidases, p1 and p7, and of their derivatives were measured over the wavelength range of 200 to 650 nm. for the two isoenzymes, it was observed that although the visible and soret bands are located at similar wavelengths, their ellipticities are different. these results suggest that the active sites are similar but that differences do exist. the results are compared with those reported for japanese radish peroxidase a and horseradish peroxidase. ...1977890576
location of feline trigeminal ganglion cells innervating maxillary canine teeth: a horseradish peroxidase analysis. 1977891695
inner and outer hair cells, differential uptake of horseradish peroxidase: a preliminary report. 1977898511
somatotopic and columnar organization in the corticotectal projection of the rat somatic sensory cortex.single injections of tritiated amino acids into the first somatic sensory area (si) of the rat neocortex result in axoplasmically transported labeling of the stratum griseum intermidiale and stratum griseum profundum of the ipsilateral superior colliculus. the terminal labeling in these layers takes the form of multiple, column-like patches. the si projection is somatotopically organized with the face and head representations projecting to an extensive anterolateral part of the colliculus and th ...1977902093
adult rat hepatocytes in primary monolayer culture. ultrastructural characteristics of intercellular contacts and cell membrane differentiations.primary monolayer cultures were obtained in 60-mm petri dishes by incubating 3 x 10(6) isolated hepatocytes at 37 degrees c in dulbecco's medium supplemented with 17% fetal calf serum. the ultrastructure of monolayer cells was examined after various incubation periods. within 4 h of plating, the isolated spherical cells adhere to the plastic surface, establish their first contacts by numerous intertwined microvilli, and form new hemidesmosomes. after 12 h of culture, wide branched trabeculae of ...1977903373
anatomical and neurobehavioral investigations concerning the thalamo-cortical organization of the rat's visual system.the organization of thalamic afferents to the rat's visual cortex was investigated autoradiographically and through the retrograde transport of horseradish peroxidase (hrp) following infections into striate and peristriate cortex. the results revealed that nucleus lateralis posterior (nlp) projects to a large peristriate cortical field that includes areas 18a, 7, and the anterior portion of area 18, and to a circumscribed temporal area corresponding to krieg's ('46a,b) area 20. the dorsal latera ...1977903426
a study of afferent input to the inferior olivary complex in the rat by retrograde axonal transport of horseradish peroxidase. 1977903429
an electron microscope study of motoneurones and interneurones in the cat abducens nucleus identified by retrograde intraaxonal transport of horseradish peroxidase. 1977903432
the morphology of spinocervical tract neurones revealed by intracellular injection of horseradish peroxidase.1. the morphology of physiologically identified spinocervical tract neurones was studied using the intracellular injection of horseradish peroxidase in anaesthetized cats.2. thirty-six spinocervical tract neurones were reconstructed from serial sections of the lumbosacral spinal cord, cut in either the transverse or longitudinal planes.3. horseradish peroxidase provided a more complete picture of the dendrites of spinocervical tract neurones than earlier experiments using procion yellow injectio ...1977903910
chronic relapsing experimental allergic encephalomyelitis. studies in vascular permeability changes.the vascular permeability in the nervous system to evans blue-albumin and horseradish peroxidase was studied in chronic relapsing eae in strain 13 and hartley guinea pigs. the disease was induced by single sensitization of immature animal and was characterized clinically by remissions and relapses. recent and old demyelinating plaques in the spinal cord were present. these showed an increased permeability to the protein tracers. the blood-brain barrier in these plaques are therefore disturbed an ...1977906801
horseradish peroxidase acute ototoxicity and the uptake and movement of the peroxidase in the auditory system of the guinea pig.when guinea pig cochlea was perfused in vivo with a solution of 1% horseradish peroxidase (hrp) in artificial perilymph, the enzyme was found in the basilar membrane, spiral limbus, some outer and inner hair cells and some supporting cells and it was gradually cleared away with time. acute signs of cell damage included swelling, vacuolization and diffuse labeling of some hair cells, but stereocilia remained normal in configuration. albino melanocytes of the spiral ligament were also damaged, and ...1977906813
absorption of horseradish peroxidase by the principal cells of the large intestines of postnatal developing rats.the absorption of horseradish peroxidase in the rat large intestine during the postnatal developing period was investigated by cytochemical and ultrastructural methods. in the ceca and ascending colons from 4-, and 5-day-old rats, intraluminaly injected peroxidase became localised on microvilli, in the intracytoplasmic network of the apical tubules and small vacuoles (tubulo-vacuolar system), in the large supranuclear vacuole, in the golgi apparatus, on the lateral and basal cell membranes of th ...1977907196
protein transport across cerebral vessels during metrazole-induced convulsions.the effects of pentylenetetrazole-induced convulsions on cerebrovascular permeability for horseradish peroxidase were studied in locally anesthetized, artificially respired, paralyzed cats. horseradish peroxidase circulated for 1, 2.5, 5, 10, 30, and 60 min and accumulated in medium-size, i.e., arterial or venous, blood vessel walls. cerebral neuropil was permeated earliest in thalamic nuclei including medial and lateral geniculate nuclei. no tight junctions containing horseradish peroxidase wer ...1977910896
thalamic inputs to the rabbit visual cortex: identification and organization using horseradish peroxidase (hrp).horseradish peroxidase (hrp) has been injected in visual cortical area 1 (v1, striate cortex) of 33 rabbits (16 received a unilateral injection, 17 bilateral injections) in order to identify its thalamic inputs and to determine their retinotopic organization. this study has shown that when hrp is injected into different portions of v1: 1. labeled cells are consistently seen in the dorsal lateral geniculate nucleus (lgnd) and are always organized into horizontal columns arranged perpendicularly t ...1977913515
adrenergic influences on the permeability of rabbit submandibular salivary glands to blood-borne horseradish peroxidase.horseradish peroxidase (hrp) administered close-arterially, has been found to enter rabbit submandibular saliva elicited by parasympathetic nerve stimulation. adrenalin, superimposed on parasympathetic nerve stimulation, increased the passage of hrp into the saliva. use of alpha- and beta-adrenoceptor agonists, either separately or together, and use of alpha- or beta-adrenoceptor antagonists together with adrenalin indicate that both alpha- and beta-receptor stimulation is necessary for this in ...1977914650
vascular reactions to horseradish peroxidase in the guinea pig.albino guinea pigs were given intradermal injections of the protein tracer horseradish peroxidase. in a 0.1 mm concentration the tracer did not increase vascular permeability to evans blue-labelled plasma proteins. in a 1 mm concentration, however, the peroxidase induced a local vascular leakage. this leakage was almost totally inhibited by pretreating the animals with acetylsalicylic acid, while antihistamine had only a weak inhibitory effect. we therefore believe that prostaglandins are import ...1977914655
uptake of horseradish peroxidase by sensory nerve fibres in vitro.after 47 3/4 hours in vitro, organotypic cultures of dorsal root ganglia of rat were exposed to horseradish peroxidase at a concentration of 4 mg/ml of nutrient medium for 15 minutes. observations on the living nerve fibres revealed pinocytotic vesicles in the growth cones which were transported toward the perikarya for several hundreds of micrometer at a rate of 0-5-10 micrometer/min. electron microscopic sections displayed uptake of peroxidase into the growth cones by means of endocytosis. the ...1977914707
localization of acetylcholine receptors in central synapses.the localization of cholinergic receptors in brain synaptosomes and in synapses of the midbrain reticular formation and hypothalamic preoptic nucleus has been demonstrated by means of a horseradish peroxidase-alpha-bungarotoxin (hrp-alpha-btx) conjugate. only a small proportion of the total number of synapses was reactive. axon terminals of reactive synapses contained primarily small clear vesicles, while synapses characterized by large numbers of dense core vesicles were unreactive. toxin-bindi ...1977914895
subdivisions of the auditory cortex of the cat: the retrograde transport of horseradish peroxidase to the medial geniculate body and posterior thalamic nuclei. 1977915045
evidence for adsorption of heterospecific host immunoglobulin on the tegument of schistosoma mansoni.mice infected with schistosoma mansoni were immunized against human type b rh-positive (b+) rbc, bovine serum albumin, or horseradish peroxidase. adult parasites, recovered by perfusion, extensively washed, and incubated in their respective antigens, selectively bound to their tegumental surfaces only those antigens to which their murine host had been immunized. all controls supported the specificity of those reactions leading to the conclusion that adult s. mansoni in mice have the ability to a ...1977915283
the identification of mossy fibres and their cells of origin in the normal and lurcher mutant mouse.the behavioural mutant mouse lurcher survives to adult life as the heterozygote (lc/+) and shows a disorder of gait. the neurological lesion has been shown to involve degeneration of purkinje cells and inferior olivary neurones (caddy and biscoe 1976). it follows that the climbing fibre input is reduced and we wished to know if the mossy fibre input was also affected. heterozygote lurcher mutants were compared with the wild type in all experiments. mossy fibre glomeruli were identified in the ce ...1977915533
ascending projections of presumed dopamine-containing neurons in the ventral tegmentum of the rat as demonstrated by horseradish peroxidase. 1977917282
acute hypertension causing blood-brain barrier breakdown during epileptic seizures.the influence of shortlasting (less than 1 min) epileptic seizures on the permeability to protein of the blood-brain barrier (bbb) was studied in rats. the protein tracer, horseradish peroxidase (hrp) was used as marker substance. monitoring arterial blood pressure (bp) and electroencephalogramme (eeg) seizures were induced electrically after hrp was given intravenously. following a single electroshock seizure slight staining of brain tissue was seen, while after 10 electroshock stimuli followed ...1977920112
a horseradish peroxidase study of the origin of central projections to the rat olfaction bulb. 1977922489
afferent connections of the nucleus raphe dorsalis in the cat as visualized by the horseradish peroxidase technique.using a retrograde tracer technique with protein horseradish peroxidase (hrp), attempts were made to determine afferent projections to the nucleus raphe dorsalis (nrd). as a control, the injection of the hrp was also made into one of the following structures adjacent to the nrd: (1) mesencephalic periaque ductal gray; (2) nucleus linearis intermedius; and (3) third cranial nucleus. the present results indicate that the nrd, particularly is rostral part, receives direct projections arising from: ...1977922504
ultrastructural tracer studies on the permeability of the malpighian tubule of the pill millipede, glomeris marginate (villers).the electron-dense tracers ferritin, and iron-dextran, and the protein horseradish peroxidase, have been used to investigate the ultrastructural basis of permeability in the upper and lower segments of the malpighian tubules of glomeris marginata. all these materials were able to cross the basal lamina and enter the tubule lumen of the upper segment, and it was established that horseradish peroxidase was able to enter the channels which interrupt the apical junctions. in the upper segment, ferri ...1977922820
penetration of systemically injected horseradish peroxidase into ganglia and nerves of the autonomic nervous system.hrp given intravenously to rats and guinea-pigs passes within 5 minutes into the extracellular spaces of the superior cervical and coeliac ganglia, reaching the spaces between neurons and their associated satellite cells and the equivalent spaces between neuronal processes and satellite cells. the sympathetic nerve trunks have a blood-nerve barrier. the myenteric plexus does not contain blood vessels, but is permeable to tracer from the extracellular spaces of the adjacent muscle layers.1977925724
tracer and freeze fracture observations on developing tight junctions in fetal rat thyroid.the development of tight junctions in fetal rat thyroid from the sixteenth to the twentieth days of gestation was examined with conventional ultrastructural methods and freeze-fracture preparations. these results were compared with those obtained using lanthanum hydroxide and horseradish peroxidase (hrp) tracers. tight junctions appear to arise on the plasma membranes of fetal thyroid cells by the aggregation and fusion of linear particle chains which appear at several discrete sites on the plas ...1977929574
[ultrastructural studies on the tight junctions of epithelial cells of the rabbit ciliary and iridial process using horseradish peroxidase as a tracer and freeze etching technique (author's transl)]. 1977930763
effect of indomethacin and atropine in experimental asthma in conscious guinea pigs.pulmonary mechanics were measured in unanesthetized guinea pigs sensitized to horseradish peroxidase (hrp) before and during two aerosolized challenges of this antigen. during the first challenge the pulmonary resistance increased in all animals. prior to second challenge the animals received either atropine (0.2 mg/kg) or indomethacin (10 mg/kg) intraperitoneally. we found that during the second challenge the indomethacin group had an increase in pulmonary resistance slightly greater or similar ...1976931927
demonstration of concanavalin a receptors on leptomonas pessoai cell memebrane.leptomonas pessoai promastigotes cultivated in a synthetic medium were agglutinated with concanavalin a (cona). agglutination was predominantly of the flagellar-flagellar type, and was inhibited with sucrose or alpha-methyl-d-mannoside. cell surface polysaccharides and/or glycoproteins were demonstrated by several cytochemical methods at the fine-structural level. a con a-horseradish peroxidase-diaminobenzidine (dab) technic was used to detect con a receptors in the pellicular membrane of the fl ...1976933086
effect of angiotensin-induced hypertension on rat coronary arteries and myocardium.acute hypertension has been produced in rats by the intravenous infusion of angiotensin amide for 4 hours. both control and hypertensive animals were injected intravenously prior to sacrifice with either horseradish peroxidase (hrp) or colloidal carbon. epicardial arteries and blocks of ventricular myocardium containing intramyocardial arteries and arterioles have been processed for electron microscopy. hrp appears to penetrate the endoethelium of epicardial arteries from control animals within ...1976937512
distribution of endogenous albumin in the rat glomerulus: role of hemodynamic factors in glomerular barrier function.using an ultrastructural immunoperoxidase technique, the distribution of endogenous albumin in the rat glomerulus was delineated under normal and abnormal hemodynamic conditions. superficial glomeruli in anesthetized munich-wistar rats were rapidly fixed in situ by applying glutaraldehyde to the renal surface. fixed tissue slices were treated with anti-rat albumin fab fragments conjugated to horseradish peroxidase (hrp), and were then subjected to the graham-karnovsky ultrastructural peroxidase ...1976940256
descending inputs to caudal cochlear nucleus in cats: a horseradish peroxidase (hrp) study.after hrp injections into the octopus cell area of the cat cochlear nucleus, only periolivary neurons of the superior olivary complex (soc) reacted. elongate neurons in the lateral periolivary nuclei (ipsilateral to the injection) and multipolar neurons in ventromedial periolivary regions (contralateral to the injection) contained granules. no neurons in the main soc nuclei or higher auditory nuclei reacted, despite a wide range of hrp concentrations. thus, neurons from the soc to the octopus ce ...1976941860
correlations between immunoglobulin- and antibody-synthesizing cells during primary and secondary immune responses of rats immunized with peroxidase.the development of cells synthesizing immunoglobulins without detectable antibody activity and of antibody-synthesizing cells was studied during primary and secondary immune responses of rats immunized with horseradish peroxidase. after primary immunization with peroxidase emulsified in freund's complete or incomplete adjuvant, the first antibody-producing cells appeared 4 days after injection. they were preceded by cells synthesizing igg and igm without antibody function, appearing 3 days after ...1976944685
immunoelectron microscopic and immunochemical demonstrations of serum proteins in the alveolar lining material of the rat means of antibodies labeled with horseradish peroxidase, autologous albumin and igg were visualized in electron microscopy on the alveolar epithelial surface of the rat lung, as a continuous cell coat, as free granular deposits, or associated with tubular myelin figures. these 2 proteins were detected by immunochemistry in alveolar washing. after ultracentrifugation of alveolar washing, they were still found in the phospholipid-rich precipitate fraction by immunization of rabbits and by immun ...1976946160
blood-aqueous barrier in newborn and young rabbits. an electron microscopic study.distribution of intravenously injected horseradish peroxidase (hrp) in the ciliary body of newborn and young (2 weeks old) rabbits was studied with the electron microscope. the reaction product was localized in all animals in the lumen of the blood vessels, in the perivascular area, in the surrounding connective tissue stroma and also in the epithelial cell layers. the peroxidase penetrated through the intercellular junctions of the pigmented cells to the intercellular space between the apical m ...1976946358
the role of suprachiasmatic nuclei of the hypothalamus in the production of circadian rhythm.(1) action potentials were recorded from single neurons in suprachiasmatic nuclei of hypothalamus in urethane anesthetized male rat. the rate of firing ranged from less than 1 to over 10/sec but was generally 4-8/sec, and it varied from one cell to another in the same animal. (2) repetitive stimulation of optic nerve or light acting on the eye augmented the activity of approximately half of the the suprachiasmatic neurons examined (67 out of 159 cells) while 37 neurons (23%) showed clear inhibit ...1976947493
competitive enzyme-liked immunoassay with use of soluble enzyme/antibody immune complexes for labeling. i. measurement of human choriogonadotropin.we described the principle of a new enzyme-immunoassay, competitive enzyme-liked immunoassay (celia), for quantitative measurement of soluble antigens and haptens. in the assay, binding of antibody to antigen-immunosorbent is competitively inhibited by the free antigen to be measured. the amount of first antibody bound to the immunosorbent is measured by an enzymatic technique in which a heterologous bridging antibody and a soluble antibody/enzyme immune complex are applied in sequence. the solu ...1976949847
cortical cells projecting to the dorsal column nuclei of cats. an anatomical study with the horseradish peroxidase technique.the retrograde transport of horseradish peroxidase (hrp) has been used to label cortical neurons which give origin to descending projections to the dorsal column nuclei (dcn) in kittens and adult cats. hrp was injected unilaterally or bilaterally into the dorsal medulla at, or rostral to, the level of the obex, and, in most cases, retrogradely transported hrp was visualized in cortical neurons by incubation of serial 40 mum coronal or sagittal frozen sections. in the remaining cases, 1 mm corona ...1976950388
vascular leakage in the dorsal root ganglia of the rat, studied with horseradish peroxidase.horseradish peroxidase (hrp) was found to pass from ganglionic blood vessels into the extracellular space of dorsal root and vth nerve ganglia within 2 min of intravenous injection in the rat. by 5 min, the tracer had penetrated into perineuronal and periaxonal spaces. a brisk macrophage response occured, and these cells rapidly engulfed the tracer so that it has almost completely disappeared from the extracellular space 2-3 hr. fenestrated blood vessels, with and without diaphragms were seen wi ...1976950578
[the influence of plasmocellular tumours on the humoral immune response in mice (author's transl)].syngeneic mice of the c3h/he strain have been inoculated with cells of the plasmocellular tumours x5563 and x5647, producing myeloma proteins of the igg and iga class respectively, and were immunized with (1) sheep red blood cells, (2) bovine serum albumin and (3) horseradish peroxydase. the immune responses of the various groups of mice were followed. the antibody activities towards all three antigens were decreased in mice carrying the igg producing tumour x5563. this effect was correlated wit ...1976952495
[assay of the activity of horseradish peroxidase antibodies using ammonium sulfate technique (author's transl)].the antigen binding capacity (abc) of mouse and rabbit antisera to horseradish peroxidase (hrp) was measured by the farr technique, originally introduced for bovine serum albumin. results obtained by this method are independent of other antibody interactions (precipitation, formation of immune complexes, effect on enzyme activity). commercially available hrp has variable solubility in ammonium sulfate solutions; almost 40% was precipitated at 50% saturation. accordingly, the fraction of hrp solu ...1976952496
a study of the permeability barrier in epidermis and oral epithelium using horseradish peroxidase as a tracer in vitro.small biopsies of skin, and of keratinized and non-keratinized oral mucosa were incubated with horseradish peroxidase, fixed and reacted to demonstrate peroxidase activity at the light and electron microscope level. in all tissues peroxidase extended through the intercellular spaces of the epithelium up to, but not into, the stratum corneum of keratinized, or the superficial layer of non-keratinized, tissue. this distribution corresponds to that seen in studies using the tracer in vivo where the ...1976952748
cat retinal ganglion cells projecting to the superior colliculus as shown by the horseradish peroxidase method.1. cat retinal ganglion cells projecting to the superior colliculus (sc) were detected in flat whole-mounts of nissl-stained retinas by means of unilateral intracollicular injections of horseradish peroxidase (hrp). 2. with the purpose of drawing a comparison between hrp-labeled ganglion cells and the remaining population of unlabeled ganglion cells, the latter were classified according to their perikaryal characteristics into three groups: large, medium and small ganglion cells. measurements of ...1976954886
immunochemical studies of horseradish peroxidase. factors affecting the inhibition of enzyme activity by specific antibody. 1976955671
horseradish peroxidase localization of masticatory muscle motoneurons in cat.horseradish peroxidase has been injected into individual masticatory muscles in young and adult cats in order to determine the topography of the corresponding groups of motoneurons in the motor nucleus of the vth nerve. the results obtained show a clear dorsoventral somatotopic distribution; the superior muscles have their motoneurons located dorsally in the nucleus and the inferior muscles ventrally; the two main jaw closers, temporalis and masseter, are represented in the dorsal and central pa ...1976957267
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