nigral projections to the inferior and the superior colliculus in the rat: a horseradish peroxidase study. 1979547209
thalamoamygdaloid connections studied by the method of retrograde transport.on the basis of retrograde horseradish peroxidase transport from nuclei ot the amygdaloid body of the rat to the thalamus, it was found that several groups of thalamic nuclei send fibers to the amygdala. these are: (i) nuclei of posterior region of thalamus and neighboring area of the tegmentum - peripeduncular nucleus, suprageniculate-limitans nucleus, (ii) midline nuclei - paraventricular nucleus, parataenial nucleus, nucleus reuniens, (ii) intralaminar nuclei - central medial nucleus, parafas ...1979547711
hydrophobic binding of phenoxyacetic and phenylacetic acids to horseradish peroxidase and human serum albumin: structure-activity relationships.studies of protein binding in homologous series of drugs are of great interest for drug research. apparent binding constants of phenoxyacetic and phenylacetic acids to horseradish peroxidase and to human serum albumin are evaluated by nmr studies and an optical method. these constants are good parameters to describe hydrophobic interactions, and the results are in a good agreement with our protein binding model described previously.1979549409
afferent and efferent connections of the area postrema demonstrated by the horseradish peroxidase method.the previous autoradiographic study of the efferent connections of the arca postrema have been completed by using horseradish peroxidase (h.r.p.) as a marking agent. these two labelling techniques have made it possible to determine two categories of structures connected with a.p.: those with afferent connections (nucleus ambiguus), or efferent connections (mesencephalic nucleus of v, locus coeruleus, griseum centrale and inferior and superior colliculi) and those having both types of connections ...1979550738
electrophysiological and neuroanatomical studies of hepatic portal osmo- and sodium-receptive afferent projections within the brain.previous investigations concerning the function of hepatic sodium and osmoreceptors indicated that the activation of these chemoreceptive structures significantly affected physiological and behavioral mechanisms related to water and electrolyte homeostasis. though anatomical studies predicted that such information should follow the previously described gustatory afferent pathy, until recently, the precise course taken by higher order hepatic afferents was unknown. the studies described herein ve ...1979553093
cells of origin of motor axons in the subdiaphragmatic vagus of the rat.the central cell groups that give rise to the motor axons that travel in the subdiaphragmatic vagus were re-examined in the rat by transecting the dorsal or ventral vagus near the stomach and incubating the nerve stump in crystalline horseradish peroxidase (hrp). an exceedingly large percentage of cells was labeled throughout the dorsal motor nucleus of the vagus (mx), with labeled cells extending even beyond the rostro-caudal limits of the nucleus usually assigned on the basis of cytoarchitectu ...1979553094
labelled trigeminal ganglion cells after injection of horseradish peroxidase in the extraocular muscles and iiird nerve of the lamb.after injection of hrp in the oculomotor nerve labelled cells were found in the dorsal most part of the ophthalmo-maxillary area of the semilunar ganglion. below this area a labelled cellular pool was detected following the injection of the enzyme in the extrinsic eye muscles. a clear somatotopic arrangement of these muscles was noticeable.1979553536
[motoneural organisation of the anterior horn with respect to the peripheral nerves in the rat (as studied by the method of retrograde transport of horseradish peroxidase)(author's transl)].the motoneuronal organisation of the anterior horn with respect to the peripheral nerves, is still imprecise due to methodological problems. the chromatolytic and golgi methods have furnished some fruitful but incomplete data. the retrograde transport of horseradish peroxidase permits to obtain both the topography of cells and their dendritic tree. with such a method we studied the cervical enlargment of the rat's spinal cord. each nerve is in relation with a column of motoneurons whose both the ...1979555020
induction of pinocytosis in rat hepatocytes by partial hepatectomy.rat hepatocytes, normally not highly pinocytic cells, becomes so after partial hepatectomy when about two-thirds of the liver is removed. droplets, up to 20 mum in diameter, develop, initially by addition to smaller pinocytic structures and later by fusion with lysosomes. the droplets contain a material with an electron microscope periodicity characteristic of fibrin; they are periodic acid schiff-positive as is plasma. it is therefore reasonable to consider plasma glycoproteins to be major comp ...1977557041
[regulation of antibody secretion: observation made on cultured lymphoid cells from immune rabbit lymph nodes].rabbits were immunized with soluble and particulate antigens (horseradish peroxidase or srbc) in complete freund's adjuvant. four to twelve weeks after footpad immunization, popliteal lymph nodes were removed and the lymphoid cells were cultured with autologous serum for 5 to 7 days. using local hemolysis assay, we observed a recrudescence of antibody secreting cells when the autologous serum present in the medium had previously been freed of antibody by specific adsorption. this phenomenon was ...1977557948
experimental spinal cord compression by epidural neoplasm.we have developed an experimental model of spinal cord compression in rats. tumor injected anterior to the t-12 vertebral body grows through the intervertebral foramina to compress the cord and produces paraplegia in 3 to 4 weeks. evidence for vasogenic edema in spinal cord compressed by tumor includes increased water content, leakage of horseradish peroxidase into gray matter, and histologic evidence of edema. the vascular supply to the cord overlying the tumor appears to be compromised. both s ...1977558545
septal afferents to the arcuate-median eminence region in the guinea pig: correlative electrophysiological and horseradish peroxidase studies. 1977562702
effect of daily ethanol ingestion on intestinal permeability to macromolecules.the effects of regular ethyl alcohol ingestion on morphological and permeability characteristics of the small intestine were assessed in mature rats using the tracer protein, horseradish peroxidase. thirty adult rats were divided into two groups and provided a standard commercial diet in pellet form. each morning, after an overnight fast, every animal in the experimental group was administered by gavage an aliquot of 20% ethanol; animals in the control group were provided aliquots of 20% sucrose ...1978563673
ultrastructural studies on blood-brain barrier dysfunction around cerebral stab wounds, aggravated by acute ethanol intoxication.ethanol-intoxicated and non-intoxicated albino rats subjected to cerebral stab wounds were injected with evans blue-labelled albumin (eba) and horseradish peroxidase (hrp) 24 hours after the injury. the tracers were injected 30 and 2 minutes before perfusion fixation with glutaraldehyde and then prepared for light microscopy and electron microscopy. there was prominent leakage of peroxidase through chains of pinocytotic vesicles and transendothelial channels from blood, through the endothelial c ...1978566999
neurones within the "chemosensitive area" on the ventral surface of the brainstem which project to the intermediolateral column.with the method of retrograde transport of horseradish peroxidase it has been demonstrated that neurones within the "chemosensitive area" of the brainstem project to the thoracic intermediolateral column. the function of these neurones is discussed in regard to the regulation of blood pressure.1978567798
proton nuclear magnetic resonance spectra of compounds i and ii of horseradish peroxidase.enzymatic reaction intermediates of horseradish peroxidase, compounds i and ii, were studied by high-resolution nuclear magnetic resonance spectroscopy at 220 mhz. the heme peripheral proton peaks were successfully obtained in the downfield region of 50 to 80 ppm from 4,4-dimethyl-4-silapentane-5-sulfonate for compound i and of 10 to 20 ppm for compound ii at ph 9.2. this indicates that no isoporphyrin appears in the catalytic cycle of the enzyme. temperature dependences of the spectra also were ...1978568936
afferent projections related to attack sites in the pontine tegmentum.attack by a cat on a rat was elicited by electrical stimulation of the ventral parabrachial nucleus or the paralemniscal pontine reticular formation. the attack was quiet and it differed from similar behavior elicited at other sites in that the cat not only bit the rat but struck it with its paw. horseradish peroxidase was deposited at the attack sites. cells sending projections to the attack sites were located in gyrus proreus, anterior, central, and medial amygdala, the bed nucleus of the stri ...1979570870
blood-brain barrier dysfunction to peroxidase after air embolism, aggravated by acute ethanol intoxication.ethanol-intoxicated and non-intoxicated rats were injected with horseradish peroxidase and evan's blue-labelled albumin and given a small air embolus in the right common carotid artery after ligation of the external carotid branch. in both ethanol-intoxicated and non-intoxicated rats, some endothelial cells, mainly in arteries and arterioles, showed a diffuse distribution of peroxidase in the cytoplasm. in some arterioles with a diffuse endothelial distribution of peroxidase there was a detachme ...1979571899
granule secretion by the luteal cell of the sheep: the fate of the granule membrane.the ultrastructure of the corpus luteum of the sheep has been examined at the mid-stage of the estrous cycle when progesterone secretion is active. secretory granules are associated with the secretion of this hormone, and the evidence indicates that the granule membrane becomes incorporated into the plasma membrane during exocytosis. further evidence of this process has been obtained from studies on the uptake of horseradish peroxidase by the luteal cells.1979572262
horseradish peroxidase/hydrogen peroxide-catalyzed binding of aromatic hydrocarbons to dna. 1979572364
barriers and transport properties of the perineurium. an ultrastructural study with 125i-labeled albuminin and horseradish peroxidase in normal and protein-deprived rats.the permeability properties of the perineurium in sciatic nerves of 12-week-old rats were studied. the penetration of 125i-labeled albumin and horseradish peroxidase into the perineurium was investigated electronmicroscopically 10, 30, and 120 min after the local extraneural application of the tracers. the autoradiographic study included age-matched protein-deprived rats. it was concluded that the perineurium acted as a diffusion barrier but also permitted a slow passage of the macromolecules in ...1979573043
morphologic and functional changes of the aortic intima during experimental hypertension.the morphology and permeability to horseradish peroxidase of the rat aortic intima have been investigated in three experimental models of hypertension having different values of plasma renin content and plasma aldosterone level. during hypertension the aortic endothelium shows three main changes: 1) increased arithmetic mean thickness, with prominent rough endoplasmic reticulum and polyribosomes; 2) the appearance of actin microfilament bundles; and 3) increased permeability to horseradish perox ...1979573070
bulbospinal projections to the intermediolateral cell column: a neuroanatomical study.the location of those neurones in the brain stem that project to the intermediolateral column (ilc) from which preganglionic sympathetic neurones have their origin was studied by the method of retrograde transport of horseradish peroxidase (hrp). in cats 30--50 nl of a 30% hrp solution was injected into the region of the ilc at t3 or l1 on one side. after a survival period of 72 h the lower brain stem from c1 to the inferior colliculi was sectioned and prepared for histological study under brigh ...1979575994
a study on the postulated transoptic regulation of the intraocular pressure.the possibility that fibers regulating the intraocular pressure originate in the hypothalamus and course in the optic nerve, has been investigated in albino rabbits with different techniques. by means of horseradish perixodase tracing-technique, oculohypothalamic but no hypothalamoocular connections were observed. the water-loading effect on the intraocular pressure was studied after unilateral optic nerve transection. the transection was performed in three experimental groups in the following w ...1978580841
the morphology of hair follicle afferent fibre collaterals in the spinal cord of the cat.1. the enzyme horseradish peroxidase (hrp) was injected into single axons that innervated hair follicle receptors to study the morphology of their collaterals in the dorsal horn of the cord. the axons were impaled near the dorsal root entrance zone in the lumbosacral spinal cord of anaesthetized cats and hrp injected by passing current through the intra-axonal micro-electrode. the morphology was revealed by subsequent histochemistry.2. thirteen hair-follicle afferent fibres were stained includin ...1977592215
movement of horseradish peroxidase in submandibular glands of dogs after ductal injection.small volumes of solutions of a tracer enzyme, horseradish peroxidase (hrp), have been injected retrogradely via the main duct of submandibular glands in dogs, and the distribution of the peroxidase reaction product within the glands has been studied light microscopically and electron microscopically at different times after the injection. tissue taken immediately after injections of 0.25 ml, given over 10 sec, showed that hrp had penetrated into the interstitial spaces of the gland. it was usua ...1977592901
localization of adductor and abductor motor nerve fibers to the larynx.knowledge of the location of motor nerve fibers to the adductor and abductor muscles of the larynx may be useful in the diagnosis and treatment of innervation disorders in this organ. anterograde degeneration and retrograde tracer anatomical techniques have demonstrated the central and peripheral positions of these two groups of motor nerve fibers in the cat. traditional nerve fibers degeneration methods applied following intracranial transection of the vagus nerve rootlets indicated that: 1) mo ...1977596775
topographical arrangement of thalamocortical neurons in the centrolateral nucleus (cl) of the cat, with special reference to a spino-thalamo-motor cortical path through the the centrolateral nucleus of the thalamus (cl) in the cat, a topographical arrangement of the thalamocortical projection neurons was demonstrated by utilizing retrograde axonal transport of horseradish peroxidase (hrp). following injections of hrp into the medial or lateral areas of the anterior sigmoid gyrus (asg), hrp-labeled neurons were located medially or laterally in the caudal levels of the cl, respectively; neurons in the central areas of the cl were labeled after injections of hrp in ...1977598437
the reorganization of synaptic connexions in the rat submandibular ganglion during post-natal development.1. the innervation of neurones in the submandibular ganglion of neonatal and adult rats has been studied with intracellular recording, and light and electron microscopy. 2. intracellular recordings from neurones in isolated ganglia from adult animals showed that about 75% of the ganglion cells are innervated by a single preganglionic fibre. 3. however multiple steps in the post-synaptic potential (about five on average) were elicited in ganglion cells from neonatal animals by graded stimulation ...1977599418
transendothelial vesicular transport of protein in brain edema induced by ultraviolet irradiation.focal vasogenic brain edema was induced by ultraviolet irradiation of the exposed cerebral cortex of 16 cats. in 5 animals horseradish peroxidase was intravenously injected at times varying from 30 min to 24 h following irradiation and allowed to circulate for 45 min. fixation was carried out by perfusion and immersion with glutaraldehyde. the tissue, part of it incubated for peroxidatic activity, was treated for electron microscopy. the uv-irradiation leads to a shallow coagulation of the super ...1977602683
the cerebellar projection from the raphe nuclei in the cat as studied with the method of retrograde transport of horseradish peroxidase.following injections of horseradish peroxidase (hrp) in the cerebellar cortex and nuclei of the cat, the distribution of labeled cells in the raphe nuclei was mapped. the findings confirm those made previously in studies of retrograde cell degeneration following cerebellar ablations (brodal et al., 1960a), and in addition reveal new details in the projection of the raphe nuclei onto the cerebellar cortex and nuclei. all the raphe nuclei except nucleus linearis intermedius and nucleus linearis ro ...1977605998
transmission of horseradish peroxidase across the small intestinal mucosa under tissue culture conditions. 1977610391
on the lysosomal function of juxtaglomerular granules.the presence of acid phosphatase, beta-glucuronidase and aryl sulfatase in juxtaglomerular cell granules (jgg) as well as the uptake and concentration of certain low molecular weight dyes by these granules have repeatedly suggested that they are akin to lysosomes. in the present experiments, rats were injected with three substances of widely different molecular weight and physicochemical properties--sucrose, iron sorbitol-citric acid complex (jectofer) and horseradish peroxidase--that are well k ...1977610707
occurrence of patulin in fruit and different varieties of apples and pears suffering from brown rot, patulin was found in about 50 p. 100 of samples investigated (about 120). patulin levels as high as 1 g/kg rotten material were found 2-3 days after the fruit was removed from the cold store where it had been stored for 5 months. a significant diffusion into the healthy plant tissue was not observed in apples, but in peaches, tomatoes and pears. vegetable products with natural patulin content: apples, pears, peaches, apricots, ...1977613910
a horseradish peroxidase study of the cerebellorubral pathway in the rat. 1978618740
uptake and fate of luminally administered horseradish peroxidase in resting and isoproterenol-stimulated rat parotid acinar cells.the formation and fate of apical endocytic vesicles in resting and isoproterenol-stimulated rat parotid acinar cells were studied using luminally administered horseradish peroxidase (hrp) to mark the vesicles. the tracer was taken up from the lumen by endocytosis in small, smooth-surfaces "c"- or ring-shaped vesicles. about 1 h after hrp administration the vesicles could be found adjacent to the golgi apparatus. at later times hrp reaction product was localized in multivesicular bodies and lysos ...1978618893
early stages of uptake and transport of horseradish-peroxidase by cortical structures, and its use for the study of local neurons and their processes. 1978621289
human marrow erythropoiesis in culture: iii. ultrastructural and cytochemical studies of cellular interactions.human erythroblasts cultured with a methylcellulose clonal assay technique were studied with ultrastructural and cytochemical methods. the intact colonies contained only erythroblasts at a similar stage of maturation, and no macrophages were identified within the colonies. the cultured erythroblasts demonstrated many of the morphologic features described in vivo. neutral and acid glycoconjugates identified on the plasmalemma stained in a similar way to that seen in vivo with a concanavalin a hor ...1978624337
immunoelectron microscopic localization of lipopolysaccharides in the cell wall of bacteroides oralis and fusobacterium nucleatum.lipopolysaccharides (lps) have been extracted and purified from two anaerobic gram-negative bacteria: bacteroides oralis and tfusobacterium nucleatum. chemical analysis of the preparations showed a great proportion of neutral sugars, mainly glucose, in lps of b. oralis. in rabbits, lps of b. oralis induced both immunoglobulin m and g antibodies in contrast to lps of f. nucleatum, to which only immunoglobulin m antibodies were produced. an immunohistochemical method with horseradish peroxidase-la ...1978624589
the morphology of group ia afferent fibre collaterals in the spinal cord of the cat.1. the enzyme horseradish peroxidase (hrp) was injected into single ia muscle afferent fibres in anaesthetized cats. subsequent histochemistry allowed the morphology of the axons and their collaterals in the lumbosacral spinal cord to be determined. 2. fifteen ia axons were stained, four from medial gastrocnemius, four from lateral gastrocnemius-soleus and seven from muscles innervated by the posterior tibial nerve. all thirteen axons that could be traced into the dorsal roots bifurcated upon en ...1978624988
cortical projections of the thalamic mediodorsal nucleus in the rabbit.the cortical projection of the thalamic mediodorsal nuclear complex (md) in the rabbit was mapped retrograde horseradish peroxidase and anterograde tritiated proline techniques. the projection field occupied the entire medial wall rostral to a mid corpus callosal level, wrapped around the frontal pole onto the lateral convexity and tailed off caudally on the dorsal bank of the rhinal sulcus. the projection of the lateral approximately one-half of md, the half which does not receive olfactory inp ...1978626902
the vestibular nuclei in the domestic hen (gallus domesticus). iv. the projection to the spinal cord.horseradish peroxidase was injected at various levels of the spinal cord of the hen and the cells in the brain stem, labeled due to retrograde transport of the tracer enzyme, were mapped with particular reference to the vestibular nuclear complex. the deiters' nuclei project ipsilaterally to the spinal cord. the projection from the nucleus deiters ventralis reaches down to the lumbosacral spinal cord. the spinal projection from the nucleus deiters dorsalis is somatotopically organized. cells loc ...1978630381
the location of spinal projection neurons in the cerebellar nuclei (cerebellospinal tract neurons) of the cat. a study with the horseradish peroxidase technique.the distribution of spinal projection neurons was studied in the cerebellar nuclei of the cat following injections of horseradish peroxidase (hrp) into the cervical, thoracic and lumbar cord. hrp-positive (labeled) neurons were found in the medial (fastigial) and the posterior interpositus nuclei on the side contralateral to the cervical injection, being most numberous in cases with injections between the c2 and the c3 segments. in the medial nucleus (m) labeled neurons were distributed in the c ...1978630384
postnatal development of rat cerebellum: massive and transient accumulation of concanavalin a binding glycoproteins in parallel fiber axolemma.modifications of protein-bound sugars during postnatal development of rat cerebellum were studied. glycoprotein-bound mannose accumulates, in the particulate fractions, at an earlier age than the bulk of glycoprotein sugar. this corresponds to a transient and massive accumulation of glycoproteins which bind to concanavalin a (con a). these glycoproteins were localized by using fluorescent con a and the horseradish peroxidase-con a method. cerebellar white matter and the molecular layer bind mass ...1978630386
visual corticopontine input to the paraflocculus: a combined autoradiographic and horseradish peroxidase study. 1978630397
uptake of horseradish peroxidase from csf into the choroid plexus of the rat, with special reference to transepithelial transport.protein uptake from cerebral ventricles into the epithelium of the choroid plexus, and transport across the epithelium were studied ultrastructurally in rats. horseradish peroxidase (hrp, mw 40,000) was used as protein tracer. steady-state ventriculo-cisternal perfusion with subatmospheric pressure (-10cm of water) in the ventricular system was applied. hrp dissolved in artificial csf was perfused from the lateral ventricles to cisterna magna for various times, and ventriculo-cisternal perfusion ...1978630593
afferent projections to the rat locus coeruleus as determined by a retrograde tracing technique.afferent connections to the rat locus coeruleus (lc), which contains exclusively noradrenergic neurons, have been traced using the technique of retrograde transport of horseradish peroxidase (hrp). in order to ensure accurate placement of adequate amounts of hrp in the lc, a microiontophoretic delivery technique coupled with single cell recording was employed. the use of electro physiological "landmarks" as aids in placing the injections is described. following hrp injections into the lc, forebr ...1978632368
association and commissural fiber systems of the olfactory cortex of the rat.the association and commissural fiber systems arising in the olfactory cortical areas caudal to the olfactory peduncle (the piriform cortex, nucleus of the lateral olfactory tract, anterior cortical nucleus of the amygdala, periamygdaloid cortex and entorhinal cortex) have been studied utilizing horseradish peroxidase as both an anterograde and a retrograde axonal tracer. in the piriform cortex two sublaminae within layer ii (iia and iib) layer iii have been found to give rise to distinctly diff ...1978632378
increased permeability to horseradish peroxidase across cerebral vessels, evoked by electrically induced seizures in the rat.under normal conditions a slight vesicular transfer of intravenously injected horseradish peroxidase (hrp) occurs across the endothelium of cerebral vessels, especially short segments of arterioles. the vesicular transport can be notably increased by chemically induced acute hypertension. in the present investigation 4 groups of animals received hrp, and the permeability of the cerebral endothelium was studied semimacroscopically, light microscopically and electron microscopically. the rats in g ...1978636839
cells of origin of the spino-facial pathway in the cat: a horseradish peroxidase study. 1978638754
afferent projections to the central nucleus of the inferior colliculus in the rat.the afferent projections to the inferior colliculus of the rat were studied using the method of retrograde transport of horseradish peroxidase (hrp). following large injections of hrp into the central nucleus, cells within the cochlear nuclei, superior olivary complex and auditory cortex were stained. within the contralateral dorsal cochlear nucleus, fusiform cells were heavily labeled. giant cells were also labeled in deeper layers. in the contralateral ventral cochlear nucleus, virtually all m ...1978638786
observations on the afferent and efferent connections of the avian isthmo-optic nucleus.the efferent and afferent connections of the avian isthmo-optic nucleus (ion) were studied using light microscopic techniques. injections of [3h]proline into the nucleus resulted in labeling of centrifugal endings in the retina at the junction of the inner plexiform layer and inner nuclear layer, but produced no other transported label to any thalamic or mesencephalic nucleus. the origin of the tectal afferents to the ion was demonstrated by means of injections of [3h]proline into the most super ...1978638787
projections of the ascending somesthetic pathways to the cat superior colliculus visualized by the horseradish peroxidase technique.projections of the somesthetic ascending pathways to the superior colliculus (sc) of the cat have been demonstrated by means of the horseradish peroxidase (hrp) tracing technique. following hrp injection in one sc, marked neurons were found in the contralateral dorsal horn and in the contralateral dorsal column nuclei, mainly the cuneate nucleus. moreover, in the contralateral trigeminal complex, the sub-nucleus caudalis of the spinal nucleus of the vth nerve, and the subnucleus oralis plus the ...1978639909
afferent fiber connections from lower brain stem to hypothalamus studied by the horseradish peroxidase method with special reference to noradrenaline innervation.attempts were made to determine the afferent projections to the anterior hypothalamus including the preoptic area from the lower brain stem by means of the horseradish peroxidase method combined with monoamine oxidase staining to identify noradrenaline (na) neurons. in addition to this technique, a histofluorescence analysis was performed. na fibers in the medial part of the anterior hypothalamus were mainly supplied by a1 and a2 na neuron groups, while the lateral part and periventricular zone ...1978639912
a ventral lateral geniculate nucleus projection to the dorsal thalamus and the midbrain in the cat.retrograde tracing experiments using horseradish peroxidase (hrp) have been utilized for demonstrating the origin of efferent projections of the ventral lateral geniculate nucleus (lgnv) in the cat. hrp-positive cells identifiable as origins of thalamic projections were found in lgnv after injections of hrp into the lateral central intralaminar nucleus. the labeled cells appeared concentrated in the medial part of the internal division of lgnv, consisting of medium-sized multipolar cells. contra ...1978639913
horseradish peroxidase application to the protein transport investigations and alkaline phosphatase ultralocalization at the kidney barrier level.horseradish peroxidase was used to the precise investigation of low molecular protein transport across the kidney barrier. simultaneously on the parallel material alkaline phosphatase activity was revealed, as this enzyme is connected with the transport processes through cell membranes. the applied reaction diaminebenzidine has shown infiltration spots for low molecular protein at the glomercular barrier and visualized tubular reabsorption of peroxidase in proximal convoluted tubule. alkaline ph ...1978640511
ultrastructural localization of thermoplasma acidophilum surface carbohydrate by using concanavalin a.surface carbohydrate, presumably the lipopolysaccharide, of thermoplasma acidophilum was visualized by means of the concanavalin a, horseradish peroxidase, and diaminobenzidine cytochemical staining procedure.1978641014
the organization of olivo-cerebellar projections in the opossum, didelphis virginiana, as revealed by the retrograde transport of horseradish peroxidase.the retrograde transport of horseradish peroxidase was utilized to map olivo-cerebellar projections in the virginia opossum. the spinal cerebellum (anterior lobe, paramedian lobule and pyramis) receives input from several separate regions in the dorsal accessory nucleus, the medial accessory nucleus and portions of the principal nucleus. evidence is present for a topographical organization whereby specific regions of the olive project to restricted longitudinal zones. the visual-auditory region ...1978641222
the vestibular nuclei in the domestic hen (gallus domesticus) iii. ascending projections to the mesencephalic eye motor nuclei.following injections of horseradish peroxidase in the oculomotor and the trochlear nuclei in the hen, the occurrence of labeled cells was plotted in the vestibular nuclei. the majority of labeled cells was localized in the superior, the medial, and the tangential nucleus. within the superior nucleus the cells were found mainly caudally, extending medially and ventrally in central areas. in the medial nucleus labeled cells were localized exclusively in its rostral half, mainly in ventrolateral re ...1978641224
superior colliculus connections with the extraocular motor nuclei in the and indirect projections from the cat superior colliculus to the extraocular motor nuclei were studied using the orthograde autoradiographic tracing method, the retrograde horseradish peroxidase technique, and golgi methods. the results show that the superior colliculus projects to the central gray matter directly overlying the oculomotor complex. this projection arises almost entirely from the rostral third of the colliculus, and it terminates most heavily over the rostral half of the oc ...1978641226
the morphology of the spinal cord efferent and afferent neurons contributing to the ventral roots of the cat.horseradish peroxidase was applied to proximal ventral roots of the coccygeal and sacral spinal cord of cats. subsequent histochemical reaction resulted in extensive staining of spinal cord neurons that had processes in the ventral roots. this procedure was used to study four issues concerning ventral root neurons. (1) extensive transverse dendritic arborizations were revealed for large and small neurons presumed to be alpha and gamma motoneurons respectively. dendrites from these neurons were f ...1978641227
oogenesis in xenopus laevis (daudin). vi. the route of injected tracer transport in the follicle and developing xenopus laevis, vitellogenin (the yolk precursor) is synthesized in the liver and transported via the circulatory system to the ovary. in order to reach developing oocytes where it is sequestered, it must exit the circulatory system and traverse several follicular tissue layers including the theca, the follicle cell layer, and the vitelline envelope. this study demonstrates this pathway by means of electron-opaque tracers, and follows the fate of heterologous macromolecules after their incorp ...1978641487
alterations in rat jejunal permeability to a macromolecular tracer during a hyperosmotic load.the effects of intraluminal hyperosmolality on the intestinal permeability of a macromolecular tracer, horseradish peroxidase, was assessed in rats by light and electron microscopic cytochemistry. after 60 minutes of an intraluminal hypertonic perfusion of 600 mosm mannitol, horseradish peroxidase was demonstrable in the intercellular spaces between adjacent absorptive epithelial cells of jejunal villi, along the microvillar brush border, in numerous pinocytotic vesicles, in multivesicular bodie ...1978642452
afferents to brain stem nuclei (brain stem raphe, nucleus reticularis pontis caudalis and nucleus gigantocellularis) in the rat as demonstrated by microiontophoretically applied horseradish peroxidase.using a retrograde tracer technique with microiontophoretically applied horseradish peroxidase (hrp), afferent projections to the brain stem raphe nuclei (br, raphe magnus, pallidus and obscurus) and to two adjacent reticular nuclei, nucleus reticularis pontis caudalis (nrpc) and nucleus gigantocellularis (ngc) were identified. the most striking difference between the afferent projections to the br and the adjacent nuclei as determined by this method is that afferents to the br originate primari ...1978646855
preparation of protein conjugates via intermolecular disulfide bond formation.conjugates of two unlike proteins can be prepared via the intermolecular disulfide interchange reaction, namely, protein a containing thiol groups reacts with protein b containing 4-dithiopyridyl groups to yield a conjugate with the release of 4-thiopyridone. thiol groups can be introduced into proteins upon amidination with methyl 3-mercaptopropionimidate ester or 2-iminothiolane, and 4-dithiopyridyl groups can be introduced into proteins with these same reagents in the presence of 4,4'-dithiod ...1978646998
prosencephalic pathways related to the paleostriatum of the pigeon (columba livia).afferent connections of the avian paleostriatal complex were traced by means of anterograde and retrograde transport of horseradish peroxidase (hrp). the paleostriatum augmentatum (pa), a cell field comparable to mammalian caudate nucleus and putamen, was found to receive projections from a distinct population of elencephalic neurons in the temporal-parietal-occipital (tpo) and lateral cortical (cdl) areas of the neostriatum. tpo neurons, in turn, were found to receive projections from the contr ...1978647399
multiple branching of cerebellar efferent projections in cats.the retrograde labeling of neurons in the deep cerebellar nuclei with horseradish peroxidase was used to compare the morphological characteristics of neurons in the dentate and interposed nuclei projecting in the cerebellothalamic, cerebello-olivary, and cerebellar nucleocortical pathways. the results from these studies demonstrated that cerebellothalamic and nucleocortical projections from the dentate and interposed nuclei originate from similar populations of spindle- and multipolar-shaped neu ...1978648599
[degradation of the parathyroid hormone in the kidney and liver (author's transl)].the cleavage of the parathyroid hormone has been reported to take place in various peripheral tissues, especially in the kidney and liver. in order to claify the mechanism of such a degradation, bovine pth (bpth 1--84) and its synthetic n-terminal peptide (bpth 1--34) labelled with 125i by the chroramine-t method (125i-bpth 1--84) and (125i-bpth 1--34) or labelled with horseradish peroxidase pox-125i-bpth 1--84 and pox 125i-bpth 1 34) were used to study the disappearance from the blood stream an ...1978648683
the permeability of a keratinizing squamous epithelium in culture.horseradish peroxidase or lanthanum was applied to the surface of keratinized oral epithelium growing in tissue culture and the extent of penetration of these substances examined with the electron microscope. both tracer substances penetrated between the superficial keratinized squames of the tissue, the lanthanum reaching the basal cell layer and the peroxidase diffusing to within 3--8 cells of the basal layer. the permeability of the keratinized layer in this epithelium is in contrast to the s ...1978649491
neuronal ceroid-lipofuscinosis. studies of granulocyte enzyme activities.neuronal ceroid-lipofuscinosis is characterized by pigmentary degeneration of the retina, psychomotor degeneration, epilepsy and intracellular deposition of ceroidlipofuscin. recent reports have suggested that deficiency of peroxidase is the basic genetic defect. however, deficiency of myeloperoxidase could be demonstrated in some but not all patients; this deficiency was noted only when p-phenylenediamine (ppd) was used as hydrogen donor and could not be confirmed with guaiacol. we found that h ...1978650251
cytoarchitecture and thalamic connectivity of third somatosensory area of cat cerebral cortex.1. the third somatosensory area (siii) was identified in the cat cerebral cortex by the recording of surface potentials evoked by deflection of a single contralateral mystacial vibrissa. a small amount of tritiated leucine was then injected at the center of the focus of evoked activity and, after a suitable survival period, the brain was prepared for autoradiography. 2. as defined by the presence of an autoradiographic injection, the siii focus lay in a cytoarchitectonic field characterized in p ...1978650267
comparison of methods for demonstrating retrogradely transported horseradish peroxidase in neurones [proceedings]. 1978650512
morphology and organization of axon collaterals from afferent fibres of slowly adapting type i units in cat spinal cord.1. the morphology of the collaterals of single axons innervating type i slowly adapting receptors was studied by using the intra-axonal injection of the enzyme horseradish peroxidase in anaesthetized cats. the axons were impaled near their dorsal root entrance zone in the lumbosacral cord. the morphology was revealed by subsequent histochemistry. 2. thirteen type i axons were stained, nine with receptors in the hairy skin and four with receptors in the glabrous foot pad skin. twelve axons could ...1978650514
studies of pulmonary innervation in the rat using horseradish peroxidase as a neuronal marker [proceedings]. 1978650517
horseradish peroxidase uptake into the rat choroid plexus epithelium, with special reference to the lysosomal system. 1978650732
glomerular filtration of proteins: clearance of anionic, neutral, and cationic horseradish peroxidase in the rat.glomerular permeability to horseradish peroxidase, a protein slightly smaller than rat albumin but similar in shape, was studied in wistar-furth rats by using a purified neutral isozyme (hrp; molecular radius, a(e) = 29.8 a) as well as an anionic succinyl-derivative (shrp a(e) = 31.8 a) and a cationized enzyme (chrp, a(e) = 30 a). the clearance rate of the proteins was determined over a 20-min period using the amounts of enzyme actually filtered (i.e., protein in the urine and protein reabsorbed ...1978651127
ascending and intrinsic projections of the superior olivary complex in the cat.the ascending and intrinsic projections of the superior olivary complex (so) in the cat were investigated by injection of 3h-leucine and horseradish peroxidase (hrp) in so and the inferior colliculus (ic), respectively. a topically arranged projection was demonstrated from the nucleus of the trapezoid body (ntb) to the ipsilateral lateral superior olivary nucleus (lso) wiht a lesser connection in the opposite direction. the medial superior olivary nucleus (mso) has a strictly ipsilateral project ...1978658183
selective binding, uptake, and retrograde transport of tetanus toxin by nerve terminals in the rat iris. an electron microscope study using colloidal gold as a tracer.a series of specific macromolecules (tetanus toxin, cholera toxin, nerve growth factor [ngf], and several lectins) have been shown to be transported retrogradely with high selectivity from terminals to cell bodies in various types of neurons. under identical experimental conditions (low protein concentrations injected), most other macromolecules, e.g. horseradish peroxidase (hrp), albumin, ferritin, are not transported in detectable amounts. in the present em study, we demonstrate selective bind ...1978659508
anatomy of germinal centers in mouse spleen, with special reference to "follicular dendritic cells".lymphocyte proliferation in germinal centers (gc's) is thought to be triggered by antigen retained extracellularly on the surface of special "dendritic" cells. the anatomy and function of these cells have not been studied directly or in detail. we therefore examined mouse spleen gc's developing in response to sheep erythrocyte stimulation. we found that distincitve "follicular dendritic cells" (fdc's) were present in both the gc and adjacent mantle region of secondary follicles. the large, irreg ...1978659510
organization and development of the brain stem auditory nuclei of the chicken: primary afferent projections.the pattern of primary auditory projections to the brain stem of young chickens was investigated using terminal degeneration methods and orthograde transport of horseradish peroxidase (hrp) or tritiated amino acid. of particular interest was the question of whether nucleus laminaris (nl) receives primary afferents. a study of silver-stained degeneration pattersn in nucleus magnocellularis (nm) and nl at three intervals following unilateral interruption of the cochlear nerve revealed that by 48 h ...1978659669
properties of the enteric nervous system: limitation of access of intravascular macromolecules to the myenteric plexus and muscularis externa.the possible presence of a blood-myenteric plexus barrier similar to the blood-nerve and blood-brain barriers was investigated. the myenteric plexus was found to be an enclosed tubular structure incompletely surrounded by a sheath of supporting cell processes. capillaries do not enter the plexus. the capillaries which supply the myenteric layer differ in structure from capillaries of other layers of the gut and are non-fenestrated. tracers, evans blue labeled albumin or horseradish peroxidase, r ...1978659670
catecholamine innervation of the basal forebrain. iv. topography of the dopamine projection to the basal forebrain and this study the location of dopamine (da) neuron perikarya in the rostral mesencephalon of the rat was determined using the glyoxylic acid fluorescence histochemical technique. subsequently the topography of the projection of these mesencephalic neurons on the basal forebrain and striatum was analyzed using the anterograde transport-autoradiographic tracing method and the retrograde transport-horseradish peroxidase (hrp) technique. the results of these anatomical studies were correlated with t ...1978659674
the morphologic basis of proteinuria in experimental chronic serum sickness glomerulonephritis. a light and electron microscopic study using horseradish peroxidase and ferritin as tracers.the morphologic basis of proteinuria in experimental chronic serum sickness glomerulonephritis in rabbits was studied by light and electron microscopy using horseradish peroxidase (effective radius 30 a; mol. wt. 40,000) and ferritin (effective radius 60 a; mol. wt. 480,000) as protein tracers. it was found that more ferritin, but paradoxically, less horseradish peroxidase gained access to the urinary space. observations made by electron microscopy appeared to indicate a decreased permeability o ...1978662050
behavioral and anatomical consequences of small intrastriatal injections of kainic acid in the rat.the effects of bilateral injections of kainic acid into the anteromedial neostriatal region were examined behaviorally and anatomically in two groups of rats. behaviorally, kainic acid injections resulted in a severe impairment of delayed alternation retention, while the ability for visual discrimination remained unaffected. anatomically it was found that axons traversing the injected area remain able to transport horseradish peroxidase. furthermore, histological examinations of the injected reg ...1978667628
origins of the parasympathetic preganglionic fibers to the cat intestine as demonstrated by the horseradish peroxidase method. 1978667629
relative mobility of con a and anionic receptor sites on the cell surface of lymphocytes. a quantitative-cytochemical following the binding of concanavalin a and cationized ferritin to the surface of normal human peripheral blood lymphocytes were followed using ultracytochemical methods. in this paper, we described a technique involving microdensitometry, which was employed in order to make a comparative cytochemical quantitation of the dense horseradish peroxidase-diaminobenzidine reaction products representing the states of concanavalin a receptors under various conditions of distribution and redistrib ...1978670683
terminal arbors of axons that have formed abnormal connections.ther terminal arbors of individual retinogeniculate axons that have been induced to grow into an inappropriate geniculate layer have been revealed for light and electron microscopic study by being filled with horseradish peroxidase. after a unilateral ocular enucleation in kittens, single axons from the surviving eye show terminal arbors not only within their own geniculate layers but also in the denervated layers. the new, abnormal arbors arise from the terminal segments of arbors that lie with ...1978675248
fine structure of tight junctions between rat choroidal cells after osmotic opening induced by urea and sucrose.after ventriculo-cisternal perfusion of hypertonic urea or sucrose, both the choroid plexus permeability to horseradish peroxidase and the structure of tight junctions between choroidal cells are modified. intercellular spaces are swollen, continuous ridges are fragmented and intrajunctional spaces are invested by many membranous particles. these morphological alterations appear to be reversible. these ultrastructural data are related to an osmotic maladjustment induced by the introduction of hy ...1978675669
limbic projections to the cat thalamus. a horseradish peroxidase study.retrograde axonal transport of horseradish peroxidase was used to identify the afferent projection systems of the anterior thalamic nuclei in the cat. the results indicate that fibres projecting to the three anterior nuclei of the thalamus arise from the hippocampal region, septum and cingulate cortex unilaterally and from the hypothalamus (mamillary nuclei) both bilaterally and unilaterally. the cells of the medial mamillary nuclei send their axons to the ipsilateral av and am nuclei whereas th ...1978676656
barriers of peripheral nerve towards exogenous peroxidase in normal and protein deprived rats.the protective barriers of the sciatic nerve in protein deprived and normal rats have been studied. horseradish peroxidase (hrp) was applied extra-neurally in rats aged between 4 and 26 weeks. the spread of the tracer was investigated 2 h, 24 h, and 3 days after application. passage of tracer into the endoneurium was found in all rats but decreased with age. within the endoneurium, hrp was phagocytized by normally occurring endoneurial histiocytes, which constitute part of the protective barrier ...1978676677
increased vesicular transport of horseradish peroxidase across the blood-brain barrier after chemical induction of hypertension. 1978676899
hypertension-induced cerebral atherosclerosis in the cholesterol-fed rabbit.foam cell lesions were found in cholesterol-fed rabbits with induced hypertension, particularly in intimal cushions at branching sites, where permeability to horseradish peroxidase was enhanced. permeability to horseradish peroxidase was enhanced at the edge of intimal cushions without foam cell accumulation. this finding suggests that permeability is increased before foam cell infiltration. no foam cell lesions were observed in the intima of cerebral arteries distant from branching sites, but i ...1978678315
afferent connections of the entorhinal area in the rat as demonstrated by retrograde cell-labeling with horseradish peroxidase.the entorhinal cortex (ec) of the rat has been divided into medial (mea) and lateral (lea) subdivisions. in order to analyze its afferent connections, small deposits of horseradish peroxidase (hrp) were placed at various loci within ec. the patterns of retrograde cell-labeling charted in 18 such cases suggested that ec is projected upon by several allocortical and subcortical structures and that there are differences in the afferent connections of the two subdivisions. thus, although hrp injecti ...1978679029
anatomical evidence of direct projections from the nucleus of the solitary tract to the hypothalamus, amygdala, and other forebrain structures in the rat.ascending projections from the caudal (general-visceroceptive) part of the nucleus of the solitary tract (nts) were studied experimentally in the rat by the aid of the anterograde autoradiographic and the retrograde horseradish peroxidase (hrp) tracer techniques. microelectrophoretic deposits of tritiated proline and leucine which involved the caudal part of the nts, the dorsal motor nucleus of the vagus (dmx), and portions of the hypoglossal nucleus, nucleus intercalatus and/or nucleus gracilis ...1978679038
localized changes in blood-brain barrier permeability following the administration of antineoplastic drugs.the effects of four antineoplastic drugs on the permeability of the blood-brain barrier to evans blue-albumin and to horseradish peroxidase were studied in cats. extravasation of tracer in brain tissue was observed only rarely following the injection of methotrexate, cyclophosphamide, or vincristine. however, 5-fluorouracil (15 mg/kg) caused localized evans blue-albumin exudation in various gray and white matter areas in 8 of 13 cats to which the evans blue was administered 7 hr after the drug i ...1978679202
an enzyme-linked immunoassay for ferritin in human serum and rat plasma and the influence of the iron in serum ferritin on serum iron measurement, during acute measure human serum ferritin and rat plasma ferritin a non-competitive enzyme-linked immunoassay has been developed using horseradish peroxidase as the enzyme. in this assay it proved necessary to use heated rat plasma to obtain reproducible ferritin values. the heating procedure caused a loss of 38% of the plasma ferritin. rat plasma ferritin values have been corrected for this loss. the standard deviation, from duplicate normal human and rat samples is 10 ng ferritin/ml serum and 69 ng/ml p ...1978679492
the prefrontal cortex of the cat: anatomical subdivisions based on retrograde labeling of cells in the mediodorsal thalamic nucleus.different areas of the frontal cortex of the cat were injected with small amounts of horseradish peroxidase. the region of labeled cells in the mediodorsal nucleus of the thalamus (md) were related to the injected areas. distinct relations between subdivisions of md and of the prefrontal cortex were established: a rather large central sector of md projects to the gyrus proreus and the anterior parts of the gyri sigmoideus, rectus, and frontalis. a narrow lateral band of anterior md neurons proje ...1978680047
pulmonary capillary and permeability during hemorrhagic previous experiments from this laboratory horseradish peroxidase was used to study the structural and functional characteristics of the normal canine pulmonary capillary membrane. the present study used the same technique to try to determine if any change occurred in the pulmonary capillary as a result of hemorrhagic shock. we found that hemorrhagic shock caused a fall (5 expt) or no change (2 expt) in estimated pulmonary transcapillary pressure based on starling's equation. however, lymphati ...1978681215
increased endocytosis with lysosomal activation in skeletal muscle of dystrophic mouse.endocytosis in dystrophic muscles was studied by a combination of biochemical, radiochemical, and light and electron microscopic techniques. it was observed that the uptake of horseradish peroxidase (hrp) and 3h-inulin in vitro was increased in leg skeletal muscles from dystrophic mice compared with littermate controls. endocytosis of hrp in vivo was also increased in dystrophic muscles. when hrp was administered intravenously, light microscopic examination of the muscles showed that the macromo ...1978681982
[horseradish peroxidase labeled sources of the spinobulbar and spinothalamic fiber systems of the cat brain].a dense accumulation of horseradish peroxidase (hrp) positive neurons was found after bulbar hrp injections in the ipsilateral dorsal grey matter and in the intermediate zone on both sides. after thalamic injections the labelled neurons were located bilaterally in the ventral part of the intermediate zone. a conclusion is made that cells of the origin of the spinobulbar and spinothalamic tracts are located in the dorsal horn and in the intermediate zone of the spinal cord.1978683371
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