uptake of horseradish peroxidase in sensory nerve terminals of mouse trigeminal nerve.uptake of horseradish peroxidase (hrp) in sensory nerve terminals in sinus hair in the nose of the mice was studied after local (s.c.) or i.v. injections. hrp spread into the sinus hair follicles and surrounded the nerve terminals of the merkel disc endings and lanceolate terminals. it was incorporated into vacuoles and vesicles of these terminals. subsequently, hrp was demonstrated in nerve cell bodies in the ophthalmo-maxillary part of the trigeminal ganglion, indicating a somatopetal axonal t ...197988862
a cerebello-pulvinar projection in the cat as visualized by the use of anterograde transport of horseradish peroxidase. 197988999
the central cervical nucleus in the cat. ii. the cerebellar connections studied with retrograde transport of horseradish peroxidase.after hrp injections in kittens and adult cats into parts of the cerebellum known to receive spinal afferents, retrogradely labelled neurones were found in the c1--c4 segments of the spinal cord, primarily in the central cervical nucleus (ccn). a few labelled neurones were also found in laminae iv and vi--ix of rexed. the results obtained in kittens and adult cats were similar. control hrp injections were made into the vestibular nuclei and the inferior colliculus. after injections mainly involv ...197989038
descending tracts of the lateral columns of the rat spinal cord: a study using the horseradish peroxidase and silver impregnation techniques.the location of the cells of origin and the projection areas of descending fibre tracts of the spinal cord lateral columns were examined in rats. unilateral micro-transections of subpopulations of lateral column fibres, at c2 or t10, with subsequent application of horseradish peroxidase to the severed axons, allowed identification, by retrograde labelling, of those cell groups projecting to the spinal cord through the lateral columns. additionally, the pattern of fibre and preterminal degenerati ...197989111
capillary permeability to interstitial microinjections of macromolecules and influence of capillary hydrostatic pressure on endothelial ultrastructure.the transcapillary flux of horseradish peroxidase (hrp, mol. diam. 50--60 a) and ferritin (mol. diam. 110--120 a), microinjected interstitially into the biceps femoris muscle of rats, was analyzed in the electron microscope. from 1 min postinjection hrp was observed to occupy endothelial plasmalemmal vesicles in all positions to be expected if a vesicular transendothelial transport of material occurs in the interstitium-to-lumen direction. permeation of hrp through the intercellular clefts of th ...197989786
a route for direct retinal input to the preoptic hypothalamus: dendritic projections into the optic chiasm.with the use of golgi, horseradish peroxidase, and electron microscopic techniques, neurons within a broad region of the preoptic hypothalamus of the mouse were shown to have dendrites that projected well into the depths of the optic chiasm. further experimental and ultrastructural investigation demonstrated synapses between these dendrites and retinal axonal boutons within the chiasm. all synapses located in the chiasm were classified as gray's type i. the possible function of these dendritic p ...197989809
alpha-fetoprotein as a carrier protein in plasma and its bilirubin-binding ability.the bilirubin-binding ability of human alpha-fetoproteins, which were purified from fetal cord serum and from ascites fluid of a hepatoma-bearing patient, was examined by the difference spectrum and the jacobsen peroxidase methods. the difference spectrum observed as a result of the specific binding of bilirubin to alpha-fetoprotein had a maximum at 482 nm, and this pattern was quite similar to that observed for serum albumin. the result obtained by the difference spectrum method showed that 1 m ...197989900
[application to the study of connections in the cns of the retrograde axonal transport of an iron-dextran complex].the retrograde axonal transport of an iron-dextran complex was observed in neurons of the substantia nigra and of the intralaminar nuclei of the thalamus, after previous injection into the striatum. the histochemical demonstration of iron is simple and rapid, and can be combined with that of horseradish peroxidase, under precise conditions in the sequence of reactions. the iron-dextran complex revealed to be a valuable material for neuronal connectivity studies in the central nervous system.197989914
retrograde axonal transport of horseradish peroxidase in regenerating sciatic nerve of the rat. 197989965
superior sensitivity of conjugates of horseradish peroxidase with wheat germ agglutinin for studies of retrograde axonal transport.we have compared the retrograde axonal transport of horseradish peroxidase (hrp), to the retrograde transport of hrp conjugated with wheat germ agglutinin (wga). morphometric studies have shown that wga-hrp conjugates were 40 times more sensitive than free hrp, in the tracing of retrograde connections from the rat submandibular gland to the superior cervical ganglion. also, wga-hrp was more sensitive than free hrp in the tracing of retrograde connections from the rat tongue to the hypoglossal nu ...197990065
observations on the orthograde and retrograde transport of horseradish peroxidase in the cat.horseradish peroxidase (hrp) was applied to the precruciate cortex of the cat. the peroxidase was either applied directly to the surface or injected by glass micropipettes in single or multiple injections. retrograde as well orthograde transport was observed by light and electron microscopy in different areas: in the thalamus: the nucleus ventralis lateralis and the nucleus centrum medianum, in the red nucleus and in the spinal cord. the distribution of hrp within the nervous elements of the nuc ...197990081
cells of origin of the spinocerebellar tract in the rat, studied with the method of retrograde transport of horseradish peroxidase.following injections of horseradish peroxidase into the cerebellum, the distribution of labeled neurons was studied in the whole length of the spinal cord of the rat. to find the ascending side of the axons, injections were made following hemisections at c1 or between c1 and c2. labeled spinocerebellar tract neurons were classified into two groups according to the axonal course in the spinal cord; one is composed of neurons with uncrossed ascending axons and the other, neurons with crossed ascen ...197990539
anatomical observations of horseradish peroxidase-filled terminal primary axonal arborizations in layer ii of the substantia gelatinosa of rolando. 197990543
application of coupled oxidation reaction to electron microscopic demonstration of horseradish peroxidase: cobalt-glucose oxidase method. 197990544
neural inputs to the prefrontal agranular insular cortex in the rat: horseradish peroxidase study.the prefrontal cortex, dorsal to the rhinal sulcus of the rat (hereinafter termed the agranular insular cortex) has been examined with the use of the retrograde transport of horseradish peroxidase. labelled perikarya were seen in the region of the ipsilateral medial forebrain bundle, consistently rostral to the caudal edge of the posterior mammillary nucleus. these cells were interpreted as being the rostral members of the a10 dopaminergic cell group. labelled cells were also seen in the dorsal ...197990546
regional topography within noradrenergic locus coeruleus as revealed by retrograde transport of horseradish peroxidase.a hitherto unsuspected degree of regional topographic organization in the noradrenergic nucleus, locus coeruleus, was revealed by the use of retrograde transport of horseradish peroxidase (hrp) from terminal areas receiving noradrenergic innervation. hrp was injected into hippocampus, hypothalamus, thalamus, caudate-putamen, septum, amygdala-piriform cortex, cerebellum and cortex. successful transport was obtained from all areas, including the caudate-putamen and cerebral cortex. the pattern of ...197990684
transganglionic and anterograde transport of horseradish peroxidase across dorsal root ganglia: a tetramethylbenzidine method for tracing central sensory connections of muscles and peripheral nerves. 197991140
[study of nervous system connections by means of axonal transport of horseradish peroxidase]. 197991355
efferents and afferents of the ventral tegmental-a10 region studied after local injection of [3h]leucine and horseradish peroxidase. 197991413
projection of neostriatal spiny neurons to the substantia nigra. application of a combined golgi-staining and horseradish peroxidase transport procedure at both light and electron microscopic type of striatonigral neuron in the rat has been characterized. golgi impregnation of striatal neurons that had been retrogradely labeled by horseradish peroxidase has shown that the medium-sized, densely spiny neurons project to the substantia nigra. some of the synapses on three of these identified striatonigral neurons have been studied in the electron microscope following replacement of the golgi deposit by means of the 'gold-toning' method. synapsing axonal boutons were found on the fol ...197991416
properties of an fc gamma-binding protein isolated from human leukemic b cells.a selectively fc gamma-binding protein was isolated from purified and radioiodinated cell membranes from two cases of b-type chronic lymphocytic leukemia and one case of b-type prolymphocytic leukemia by binding to igg aggregates, horseradish peroxidase-anti-peroxidase igg complexes, and sheep erythrocyte membrane sheets densely coated with igg. this protein could not be isolated from the cell membranes of an fc gamma-receptor-negative chronic lymphocytic leukemia of the t type or from membranes ...197991652
sensitivity of the short-range spinal interneurons of the cat to experimental spinal cord trauma.the technique of retrograde axoplasmic transport was used to demonstrate the effect of experimental spinal cord injury on the spinal interneurons in the upper lumbar and lower thoracic segments of cats. force of varied intensity was applied to the dorsal surface of the spinal cord and horseradish peroxidase (hrp) was injected into the next caudal segment. a large impact (250 to 350 gm-cm) inducing permanent paraplegia of the hind legs blocked the axoplasmic transport instantaneously in both cran ...197991677
an electron microscopic study of neurones in the lateral geniculate nucleus of the monkey labelled by retrograde transport of horseradish a previous report horseradish peroxidase (hrp) reaction product has been described as accumulating in a restricted part of neurones in the lateral geniculate nucleus following injections of hrp into the visual cortex of the primate. with the aid of long series of ultrathin sections from the same material labelled neurones have been partially reconstructed in the present study, and it has been confirmed that hrp reaction product aggregates as a group of electron-dense lysosome-like bodies whic ...197992349
selective retrograde transsynaptic transfer of a protein, tetanus toxin, subsequent to its retrograde axonal transport.the fate of tetanus toxin (mol wt 150,000) subsequent to its retrograde axonal transport in peripheral sympathetic neurons of the rat was studied by both electron microscope autoradiography and cytochemistry using toxin-horseradish peroxidase (hrp) coupling products, and compared to that of nerve growth factor (ngf), cholera toxin, and the lectins wheat germ agglutinin (wga), phytohaemagglutinin (pha), and ricin. all these macromolecules are taken up by adrenergic nerve terminals and transported ...197992475
myoglobin as an ultrastructural probe for studying permeability of the nephron.hemeproteins, like cytochrome c (12,500 m.w.; karnovsky and rice, 1969) and myoglobin (17,816 m.w.; anderson, 1972; simionescu et al., 1973) are advantageous over the true peroxidases with larger molecular weights (e.g. horseradish peroxidase, ca. 40,000 m.w) as ultrastructural probes in that they do not elicit vascular leakage in the inflammatory response (cotran and karnovsky, 1967) and are relatively nontoxic immunologically inert substances. the main disadvantage in using cytochrome c and my ...197992492
uptake and retrograde axonal transport of horseradish peroxidase in normal and axotomized motor neurons during postnatal development.the axonal uptake and somatopetal transport of horseradish peroxidase (hrp) was studied during early postnatal development of facial neurons in mice and rats. hrp injected systemically or locally into the muscles of the vibrissae, diffused into the region of the immature neuromuscular junction and was incorporated into vesicles in the axon terminals on the first and third postnatal days, at a time when synaptic vesicles were already present. hrp later was found in the nerve cell bodies of the fa ...197992768
osmiophilic reagents in electronmicroscopic histocytochemical staining methods on undisrupted tissues, stabilized by chemical fixation, potentially offer perhaps the most reliable approach to the study of the enzymes of the cell with relation to its ultrastructure. the atoms which, for the most part, comprise the biomacromolecules and enzymes of cells and tissues contribute little to their inherent electron opacity or ability to scatter electrons differentially. the latter property of a substance is responsible for its observation ...197992799
cells of origin of the hippocampal afferent projection from the nucleus reuniens thalami. a combined golgi-hrp study in the rat.neurons of the nucleus reuniens thalami stained with golgi methods are compared to cells in this nucleus labelled in retrograde fashion after hippocampal injections of horseradish peroxidase. the cellular morphology ranges from fusiform to multiangular with most cells showing radiating processes characteristic of neurons in the reticular core. dendrites are long and relatively smooth, with a few sparsely distributed spinous processes. these cells are comparable to the cholinergic cells of the me ...197993025
the cerebellar corticonuclear and nucleocortical projections in the cat as studied with anterograde and retrograde transport of horseradish peroxidase. i. the paramedian lobule. 197993421
an approach to tracing neuron networks in the cerebral cortex and basal ganglia. combination of golgi staining, retrograde transport of horseradish peroxidase and anterograde degeneration of synaptic boutons in the same material. 197993712
retrograde transport of horseradish peroxidase in sciatic nerve of rats and dystrophy mice.the relationship between nerve or muscle activity and retrograde hrp transport was studied in rat sciatic nerve and in dystrophy mice. sciatic nerve stimulation facilitated transport to 250% of control level, while colchicine decrease transport depending on dosage. tendonectomy as well as colchicine treatment ultimately decreased hrp transport, but before the decrease, there was a transient facilitatory phase in both colchicine treated and tendonectomized rats. in dystrophy mice, almost twice as ...197993715
gastric afferents to the nucleus of the solitary tract in the cat.afferent projections of the vagus nerve (x) were investigated following nodose ganglion injections of [3h]leucine or horseradish peroxidase (hrp) and stomach wall injections of hrp. ganglionic injections demonstrated a projection to the solitary nucleus (sn) including the part designated as the area subpostrema (asp). a projection to the area postrema was also seen following [3h]leucine injections. stomach wall injections of hrp labelled fibres and terminals bilaterally in the asp and more ventr ...197993717
a horseradish peroxidase study of primary afferent projections to the medullary cuneate nucleus in the rat.hrp was used to label first order afferent cells of the dorsal root ganglia which synapse at the cuneate nucleus. the results show that it is a population of predominantly large cells that project monosynaptically to the medullary cuneate nucleus and thus support anatomical and physiological studies showing that directly projecting axons to the dorsal column nuclei are larger, faster and conduct information from a select class of sensitive cutaneous mechanoreceptors. the finding, that approximat ...197993721
distribution of carotid sinus nerve afferent fibers to solitary tract nuclei of the cat using transganglionic transport of horseradish peroxidase.the distribution of carotid sinus nerve (csn) afferent fibers to the solitary tract and its various nuclei has been determined using transganglionic transport of horseradish peroxidase (hrp). the ipsilateral solitary tract was labeled and, in addition, the heaviest labeling was seen dorsomedial within the medial solitary nucleus, as well as just lateral to the tractus solitarius in the lateral solitary nucleus. bilateral labeling was present caudal to the obex in both the medial and commissural ...197993722
somatotopy in the phrenic motor nucleus of the cat as revealed by retrograde transport of horseradish peroxidase.cats received unilateral or bilateral horseradish peroxidase (hrp) injections into various portions of the diaphragm. in two experiments one of the cut cervical roots of the phrenic nerve was immersed in hrp. the phrenic motoneurons located in the fifth and occasionally the fourth cervical segment send their axons, via the upper phrenic root, to pars sternalis and pars costalis of the diaphragmatic dome whereas the neurons of the sixth segment innervate preferentially the dorsal portion both cru ...197993723
location of bladder preganglionic neurons within the sacral parasympathetic nucleus of the cat.sacral parasympathetic preganglionic neurons innervating the urinary bladder were labelled by applying horseradish peroxidase (hrp) to branches of the pelvic nerve at the neck of the bladder. bladder preganglionic cells were located primarily in the intermediolateral grey matter of sacral cord segments 1, 2 and 3 and extended from the ventral end of the central canal to the inferior margin of the dorsal horn. dendrites extended into the dorsolateral funiculus, toward the lateral edge of the dors ...197993725
superior colliculus efferents to the hypothalamus.the projection of the superior colliculus to the hypothalamus was studied in the rabbit and rat with light and electron microscopic techniques. this projection system, as demonstrated with the anterograde transport of tritiated leucine, proline, adenosine or horseradish peroxidase, is primarily to dendrites located in the ipsilateral optic and supraoptic tracts. the dendrites originate from cells located in the lateral and anterior hypothalamic areas, retrochiasmatic area and supraoptic nucleus. ...197993726
double labelling of blanched neurons in the central nervous system of the rat by retrograde axonal transport of horseradish peroxidase and iron dextran complex.the retrograde axonal transport of an iron-dextran complex leads to a labelling of neural cell bodies in the central nervous system (cns) of the rat. this tracer and horseradish peroxidase (hrp) can both be demonstrated histochemically in same cell bodies of intralaminar thalamic neurons in the central lateral nucleus, after injection of iron-dextran in the striatum and injection of hrp in the motor cortex. this is made possible by processing the sections first for hrp and then for ferric ions b ...197993728
macrophage-lymphocyte clusters in the immune response to soluble protein antigen in vitro. ix. antigen-pulsed macrophages as a tool for specific absorption of cluster-initiating t cells.t-cell populations from guinea-pigs sensitized to the protein antigens purified protein derivative of mycobacterium tuberculosis, ovalbumin, or horseradish perioxidase can be selectively depleted of cells capable of initiating antigen-specific macrophage-lymphocyte clusters in vitro. the depletion is achieved by incubating the t cells on a monolayer of antigen-pulsed macrophages in a petri dish for some hours and then gently aspirating the cells not adhering to the bottom of the dish. when subse ...197994461
[lectin binding sites of the human pituitary adenoma cells as revealed by the ferritin- and horseradish peroxidase-labeled techniques (author's transl)]. 197994659
projections of raphe neurones to the cerebellar vermis cortex in the rabbit. a horseradish peroxidase (hrp) study. 197995045
computer-controlled double-beam scanning microspectrophotometry for rapid microscopic image reconstructions.a method for automated collection of various specific data from an entire microscopical preparation and their quantitative evaluation is described. its application to the study of neuronal connections is discussed in some detail. brain sections are scanned using a computer-controlled microscope for reflectance, fluorescences or absorbance signals. two illuminating beams are used, one of them being amplitude modulated. by means of a synchronous detection the two signals are recorded simultaneousl ...197995455
[localization of motoneurones of retractor bulbi muscles by retrograde transport of exogenous peroxidase in cats].retrograde transport of exogene horseradish peroxidase has been used to localize the retractor bulbi muscle motoneurones. these motoneurones are located ventro-laterally in the tegmental reticular field; they are grouped in a rostro-caudal column above the superior olive. they correspond to the accessory nucleus of the vie nerve described by old anatomists.197896996
osmotic opening of the blood-brain barrier.the blood-brain barrier at cerebral blood vessels is due to a continuous lining of endothelial cells, which are connected by tight junctions that restrict intercellular diffusion. the endothelium excludes most water-soluble solutes and proteins but supports facilitated stereospecific transport of monosaccharides and large neutral and basic amino acids. the barrier in different species can be made permeable by infusing a hypertonic solution of urea or arabinose into the internal carotid artery. e ...197897064
experimental injury of the optic nerve with optic disc ultrastructural study utilizing horseradish peroxidase was performed to determine the mechanism and consequences of leakage of vascular protein following injury of the optic nerve. unilateral optic nerve injuries were produced in four rhesus monkeys by making a cautery lesion on the retrobulbar portion of the optic nerve. optic disc changes were followed with stereo fundus photography and fluorescein angiography. three to 14 days after injury horseradish peroxidase was given intravenously and ...197898667
[retrograde axonal transport of horseradish peroxidase along the striato-thalamic pathways of cats]. 197898719
an ontogenic study of the acute inflammatory response in the fetal rhesus monkey. ii. the mediation of increased vascular permeability by histamine, compound 48/80 and bradykinin.vascular permeability as a function of age was evaluated quantitatively and morphologically in skin of fetal rhesus monkeys given vasoactive mediators. the response was examined at 90, 120 or 150 days of gestation or at birth and compared to the response of yearling rhesus monkeys. skin sites were challenged with histamine, compound 48/80 or bradykinin. evans' blue and horseradish peroxidase were used as markers of vascular integrity. evans' blue was quantitated spectrophotometrically. vascular ...197898895
a horseradish peroxidase-autoradiographic study of parietopulvinar connections in saimiri sciureus.descending connections from parietal cortex to pulvinar in squirrel monkey were investigated with the autoradiographic method. somatosensory areas i (si) and ii (sii) were found to project to the oral (puo) and medial (pum) subdivisions of the pulvinar. projections from the posterior parietal region were recorded in circumscribed areas of pum and the lateral (pul) and inferior (pui) subdivisions of pulvinar. retrograde labeling with horseradish peroxidase (hrp) demonstrated that rostral parietal ...197899328
a comparative study of the neurons of origin of the spinocerebellar afferents in the rat, cat and squirrel monkey based on the retrograde transport of horseradish peroxidase.the cell bodies of the neurons of the spinocerebellar pathways were examined using large injections of horseradish peroxidose into the cerebellum. sections of each spinal segment were examined with both the dab and the de olmos o-dianisidine techniques. results common to all three species were found. in clarke's nucleus, the central cervical nucleus, and the spinal border cells there were many heavily labeled cells. clarke's nucleus was found to project primarily ipsilaterally; the spinal border ...197899460
the neuroanatomical organization of pathways between the dorsal lateral geniculate nucleus and visual cortex in old world and new world primates.pathways between the dorsal lateral geniculate nucleus (dlgn) and visual cortex in old world (macaca, papio, erythrocebus, cercopithecus) and new world (saimiri, cebus) primates were studied after injections of horseradish peroxidase and h3 or s35 amino acids into the dlgn or visual cortex. trans-synaptic autoradiography was also used to study these pathways after an injection of h3 proline-fucose into one eye. the subsequent autoradiographs of visual cortex showed that old world primates have s ...1978100530
organizational features of the cat and monkey cerebellar nucleocortical projection.the organization of the cerebellar nucleocortical projection in the cat and the monkey has been studied using orthograde and retrograde neuroanatomical tracing techniques. injections of tritiated leucine in the cat cerebellar nuclei orthogradely labeled nucleocortical fibers throughout their course to the cerebellar cortex. their branch points in the corpus medullare, in the folial white matter, and in the granular layer were evident from the dense, continuous distribution of silver grains overl ...1978100532
the placental fcgamma receptors studied using immune complexes of peroxidase.immune complexes of horseradish peroxidase (hrp) and rabbit igg antibodies to hrp were used to study the fcgamma receptors in normal human placenta. cryostat sections of placental tissue were incubated with the complexes, and the peroxidase activity was revealed histochemically. the bound complexes were localized to the apical surface of the trophoblasts and endothelial cells of the fetal stem vessels. binding also occurred within the wall of some fetal vessels, to stromal cells and occasionally ...1978100870
spinothalamic tract neurons in the substantia gelatinosa.the substantia gelatinosa of the mammalian spinal cord is generally believed to be a closed system; that is its neurons are thought to project only to the substantia gelatinosa of the same or the contralateral side. experiments in monkeys, using injections of the marker enzyme horseradish peroxidase, show that at least some neurons of the substantia gelatinosa project to the thalamus and thus belong to the spinothalamic tract. such neurons include two cell types intrinsic to the gelatinosa, the ...1978102034
oculomotor nucleus afferents in the monkey demonstrated with horseradish peroxidase.the afferents to the oculomotor nucleus were examined in the macaque monkey by means of horseradish peroxidase. the demonstration of afferents from the contralateral interstitial nucleus of cajal, and from the nearby rostral interstitial nucleus of the medial longitudinal fasciculus, predominantly ipsilateral to the injection site, confirms the significant role of these two regions as premotor structures. hrp label in the pretectal area was strictly confined to the olivary nucleus and the corres ...1979102412
the identification of relay neurons in the dorsal lateral geniculate nucleus of monkeys using horseradish peroxidase.intraaxonal retrograde transport of the protein horseradish peroxidase (hrp) was used to identify relay neurons in the dorsal lateral geniculate nucleus (lgn) of owl (aotus trivirgatus) and rhesus (macaca mulatta) monkeys. in both species, from 94.1-98.6% of the neurons within columns extending through both parvocellular and magnocellular layers were labeled following injection of hrp into striate cortex. labeled neurons were also identified in the thin ventral-most s(0) layers. although most of ...1978102664
projections to the visual cortex in the golden hamster.retrograde transport of horseradish peroxidase (hrp) was used to determine the origins of afferent connexions to the visual cortex (areas 17, 18a and 18b) in the hamster. the distribution of neurons projecting to the visual cortex from other cortical areas, from the thalamus and from the brainstem was studied using a computer technique for three-dimensional reconstruction. there is a topographically organized projection from the dorsal lateral geniculate nucleus to area 17, but probably to no ot ...1979102666
mediation of pinocytosis in cultured arterial smooth muscle and endothelial cells by platelet-derived growth factor.pinocytosis was measured in monkey aortic smooth muscle cells (smc), bovine aortic endothelial cells, and swiss 3t3 cells in culture as cellular uptake of [u-(14)c]sucrose and horseradish peroxidase (hrp) from the tissue culture medium. monkey arterial smc and swiss 3t3 cells were maintained in a quiescent state of growth at low cells density in medium containing 5 percent monkey plasma-derived serum (pds). replacement of pds with 5 percent monkey whole blood serum (wbs) from the same donor, or ...1978103882
anatomical localization of corneal afferent cells in the trigeminal ganglion.corneal afferent cells were identified in the trigeminal ganglion in the cat and monkey by the method of retrograde axonal transport of horseradish peroxidase. the corneal cells were found only in the ophthalmic portion of the ganglion and were scattered randomly within that division. these data are compatible with clinical and other experimental descriptions of the organization of the trigeminal ganglion and sensory root.1978104187
columnar cortico-cortical interconnections within the visual system of the squirrel and macaque monkeys.cortico-cortical interconnections within the visual cortex of squirrel monkeys (saimiri sciureus) were studied by means of single or multiple injections of [3h]leucine or combined [3h]leucine and horseradish peroxidase into the dorsolateral striate and prestriate cortices. injections of [3h]leucine were also made into the dorsolateral striate cortex of a macaca speciosa for comparison. the results indicated that in saimiri the dorsolateral prestriate cortex is precisely and reciprocally connecte ...1979104776
the organization of feline entopenduncular nucleus projections: anatomical studies.the organization of entopeduncular nucleus (epn) projections was studied in cats using autoradiographic and horseradish peroxidase (hrp) techniques. in autoradiographic studies, epn axons were found to terminate in a j-shpaed region in the dorsal and medial part of the ventral anterior nucleus (va) and the rostral portion of the adjacent ventral lateral nucleus (vl). epn axons also terminated in the rostral portion of the centrum medianum (cm), the ventrolateral portion of the lateral habenular ...1979105022
[the catecholaminergic connections of the visual system of the albino rat (author's transl)].by using the glyoxylic acid method of de la torre and surgeon (1976), occurrence, distribution and origin of catecholamines were investigated in the visual system of the albino rat. a dense network of green fluorescent terminals and preterminals could be detected in the dlgn. the spreading of terminals of catecholaminergic fibres had been observed in laminae i and ii of the visual cortex. investigation of lamina i with the golgi-kopsch method showed axons the thickness of which was below 1 micro ...1978105553
origin of the fornix system in the squirrel monkey.wit the use of the horseradish peroxidase retrograde axonal transport technique this experiment attempted to identify the cell bodies of origin whose axons supply the septal area, mammillary bodies and the anterior thalamic complex in the squirrel monkey. it was noted that axons which innervate the anterior thalamic complex arise predominantly from the deepest cell layer of both the retrosplenial cortex and adjoining portions of subicular cortex. hippocampal afferents to the mammillary bodies ar ...1979105779
the pontocerebellar projection in the rhesus monkey: an experimental study with retrograde axonal transport of horseradish peroxidase. 1979106327
connections of areas 3b and 1 of the parietal somatosensory strip with the ventroposterior nucleus in the owl monkey (aotus trivirgatus).anatomical tracers were injected into electrophysiologically defined sites in somatosensory cortical area 3b (si proper) and area i (posterior cutaneous field) of owl monkeys after these cortical subdivisions had been extensively explored in microelectrode mapping experiments. these mapping experiments revealed that both areas 3b and 1 contain complete and separate representations of the body surface (merzenich et al., '78). restricted injections of the retrograde tracer, horseradish peroxidase ...1979107204
ultrastructural localisation of lipopolysaccharide-binding sites with peroxidase-conjugated lipopolysaccharides.the localisation of lipopolysaccharide-binding sites on erythrocytes with peroxidase-coupled lps is described. lps was isolated from fusobacterium nucleatum (fus mc-8) by phenol-water extraction. the lps was coupled to horseradish peroxidase by the two-step method of avrameas and ternynck (1971). the biological and serological activities of the conjugated lps were compared with those of the native material. peroxidase could be coupled to lps without significant loss of endotoxic or serological a ...1979107240
localization of neurons supplying the temporalis muscle in the rat and monkey.following injection of horseradish peroxidase (hrp) solution into the rat and monkey temporalis muscle, a similar pattern of nuclear representation was found in the motor trigeminal nucleus. labelled neurons were seen throughout the rostro-caudal extent of the ipsilateral motor trigeminal nucleus, in its dorsal, intermediate and ventral portions and its dorsomedial, ventromedial and ventrolateral corners. in addition to the motor trigeminal nucleus labelled neurons were also found in the ipsilat ...1979107492
spinal termination of functionally identified primary afferent neurons with slowly conducting myelinated fibers.single primary afferent myelinated fibers from cutaneous receptors of cat and monkey were functionally identified by recording from the spinal cord with micropipettes filled with horseradish peroxidase (hrp). relatively slowly conducting fibers (less than 40 m/sec) from high threshold mechanoreceptors (mechanical nociceptors) and two types of low threshold mechanoreceptor (d-hair and field) were selected for staining. iontophoresis of the hrp and subsequent histochemical reaction stained the axo ...1979109477
the labeled antigen method of immunoenzymatic staining.a two stage immunohistological technique (the "labeled antigen" procedure) has been assessed for the detection of a variety of human and animal cytoplasmic constituents in tissue sections. in this method specific antiserum is followed by antigen complexed to horseradish peroxidase or to alkaline phosphatase. the primary antibody acts bivalently, linking the labeled antigen to antigen in the tissue section. the major advantage of this technique is that nonantigen specific antibody in the primary ...1979109496
localization of motoneurons innervating the posterior belly of the digastric muscle: a comparative anatomical study by the hrp method.motoneurons innervating the posterior belly of the digastric muscle were identified in the monkey, cat, dog, guinea pig and rat by the hrp method. after injections of horseradish peroxidase (hrp) into the posterior belly of the digastric muscle, two groups of hrp-labeled motoneurons were observed; the rostral group was seen as a small cluster of neurons in the lateral reticular area along the medial border of the descending root of the facial nerve, and the neurons of the caudal group were distr ...1979111172
the afferents and projections of the ventroposterolateral thalamus in the seven monkeys (m. fascicularis), recordings were made from neurones in subnuclei vplo and vplc of the thalamus. the peripheral inputs to these cells from the forelimb were established by stimulating muscle, skin and joint nerves, and cutaneous receptive fields were examined by natural stimulation of the skin. the projection of the same cells to motor and sensory cortex was examined by stimulation of the cortex to demonstrate antidromic responses and by retrograde axonal transport of horseradi ...1979111956
sensitivity in horseradish peroxidase neurohistochemistry: a comparative and quantitative study of nine methods.nine currently available methods for hrp neurohistochemistry have been compared with each other on matching tissue sections from four rats and four rhesus monkeys. the nine methods investigated in this report are the diaminobenzidine (dab) procedures of lavail jh and lavail mm (j comp neurol 157:303, 1974), of adams jc (neuroscience 2:141, 1977) and of streit p and reubi jc (brain res 126:530, 1977); the benzidine dihydrochloride (bdhc) procedures of mesulam m-m (j histochem cytochem 24:1273, 19 ...1979113450
a comparison of different enzyme-antibody conjugates for enzyme-linked immunosorbent assay.calf intestinal alkaline phosphatase (ciap)- and horseradish peroxidase (hrp)-linked goat antibody (ab) conjugates have been prepared by two procedures. one procedure is by glutaraldehyde coupling of the proteins; the other is by intermolecular disulfide bond formation of the appropriately modified proteins. the conjugates, specific for rabbit igg, were tested for their effectiveness as reagents in enzyme-linked immunosorbent assays for ragweed antigen e specific rabbit antibody. the ciap-ab con ...1979113461
visual thalamocortical connections in sheep studied by means of the retrograde transport of order to study the visual thalamocortical connections in the sheep, horseradish peroxidase (0.3--0.5 microliter of a 30% solution) has been injected in the gyri marginalis, ectomarginalis medius pars medialis, ectomarginalis medius pars lateralis and ectosylvius caudalis. the results show that: (1) the dorsal lateral geniculate nucleus (lgnd) projects to the former three gyri. dorsal parts of the lgnd project to caudal areas, whereas its ventral parts project to rostral areas of these gyri; m ...1979114554
cells of origin of long descending propriospinal fibers connecting the spinal enlargements in cat and monkey determined by horseradish peroxidase and electrophysiological techniques.the cells of origin of the long descending propriospinal tract (ldpt) in the cervical enlargement were studied in cat and monkey by using the retrograde transport of horseradish peroxidase (hrp). their distribution was confirmed electrophysiologically in cat by recording their antidromic action potentials. in cats and monkeys unilateral injections of hrp were made into the gray matter of the lumbosacral enlargement, but there was some spread to the contralateral side. in cats labeled somas were ...1979114558
alterations in human monocyte structure and function induced by incubation with gold sodium thiomalate.the effect of in vitro indubation with gold sodium thiomalate (gst) on the morphology and functional activity of human mononuclear phagocytes (mphi) was examined. human peripheral blood mphi that had been incubated with gst (25 micrograms/ml) for 4 days developed large intracytoplasmic vacuoles. similar vacuolization developed after incubation with gold chloride but not with thiomalic acid. gst pre-incubation also induced a number of functional alterations in mphi. gst incubation had little effe ...1979114643
vestibular projections to the monkey thalamus: an autoradiographic study.vestibulothalamic projections were studied in the monkey (macaca mulatta) by injecting anerograde trace substances (radioactive leucine and proline) into the vestibular nuclear complex. terminal labelling was found bilaterally mainly in the nucleus ventroposterior lateralis pars oralis (vplo) and to a lesser extent in the nucleus ventroposterior inferior (vpi) and nucleus ventralis lateralis pars caudalis (vlc). the labelling was sparse, and scattered over wide areas. the vestibular origin of th ...1979115546
enzyme-linked immunosorbent assay for pseudomonas aeruginosa exotoxin enzyme-linked immunosorbent assay (elisa) is described for pseudomonas aeruginosa exotoxin a. a double antibody sandwich method was used, employing polyvinyl microtiter plates as the solid phase, a primary coat of monospecific rabbit antitoxin serum, an outer layer composed of a horseradish peroxidase-sheep antitoxin immunoglobulin g conjugate, and an ortho-phenylene-diamine substrate. absorbance (optical density) of hydrolyzed end product was read spectrophotometrically at 492 nm. elisa dete ...1979115899
[application of horseradish peroxidase method in the trigeminal nerve system]. 1979115953
laminar origins and terminations of cortical connections of the occipital lobe in the rhesus monkey.cortical connections within the occipital lobe (areas 17, 18 and 19) of the rhesus monkey are investigated with the autoradiographic and horseradish peroxidase procedures. two efferent systems, each with a specific laminar organization, are observed. (1) rostrally directed connections, from area 17 to 18, area 18 to 19 and area 19 to the inferotemporal region (area te), originate from neurons in layer iiic (and, in area 19, from a small complement of neurons in layer va), and terminate in and ar ...1979116716
diffusion in gel-enzyme linked immunosorbent assay (dig-elisa): a simple method for quantitation of class-specific antibodies.a new method for quantifying class-specific antibodies is presented. the method has been named diffusion-in-gel-enzyme-linked-immunosorbentassay (dig-elisa), and is briefly as follows. antiserum ia allowed to diffuse from wells in a gel layered over an antigen-coated plastic surface. the gel is then removed and the preparation is incubated with enzyme-conjugated anti-immunoglobulin. the enzyme is then visualised in situ by a colour reaction produced by pouring a substrate-containing gel over the ...1979117055
[morphofunctional interrelationships between the hippocampus and hypothalamus in rats].evoked reactions of the hypothalamic arcuate and medial preoptic nuclei neurons were recorded when the hippocampus was stimulated by single stimuli in anaesthetized rats. in the arcuate nucleus phasic responses and primary inhibition were found to be dominant and in the medial preoptic nucleus--both phasic and nonspecifical responses. after injection of the horseradish peroxidase into the stimulated hippocampal region stained cells were found in the nuclei of the mammillary complex, mediobasal h ...1979117390
cortical projection to hand-arm motor area from post-arcuate area in macaque monkeys: a histological study of retrograde transport of horseradish four macaque monkeys horseradish peroxidase (hrp) was injected into physiologically defined hand-arm motor area. ipsilaterally, hrp labeled neurons were found in both upper and lower limbs of the posterior bank of the arcuate sulcus and in an area surrounding the arcuate spur. contralaterally, labeled neurons were found in the same areas, though less dense in concentration. labeled neurons were found mostly in layer iii of the cortex.1979117403
cathepsin d: ultra-immunohistochemical localization in dentinogenesis.cathepsin d was purified from rat liver using a new affinity chromatographic method, based on the coupling to the specific inhibitor pepstatin. this preparation was used for the production of specific antibodies from rabbit. the purified igg fraction was conjugated to horseradish peroxidase in a two-step coupling procedure and used for electron microscopic immunohistochemistry of the odontoblast-predentine region of the rat incisor. precipitates, indicating the presence of cathepsin d, were seen ...1979117889
the cells of origin of the primate spinothalamic tract.spinothalamic tract cells in the lumbar, sacral and caudal segments of the primate spinal cord were labelled by the retrograde transport of horseradish peroxidase (hrp) injected into the thalamus. the laminar distribution of stained spinothalamic cells in the lumbosacral enlargement differed according to whether the hrp was injected into the lateral or the medial thalamus. lateral injections labelled cells in most laminae, but the largest numbers of cells were in laminae i and v. the highest con ...1979118192
a comparison of the ability of beta-galactosidase and horseradish peroxidase enzyme-antibody conjugates to detect specific antibodies.the ability of two different enzyme-antibody conjugates to detect specific antibodies has been compared. beta-galactosidase was conjugated to antibodies raised against rabbit fc fragments using m-maleimidobenzoyl-n-hydroxysuccinimide ester (mbs). horseradish peroxidase (hrp) was conjugated to part of the same batch of antibodies using the periodate method. the beta-galactosidase and hrp labels enabled detection of approximately 8 fmoles and 4 fmoles respectively of human growth hormone (hgh) ant ...1979118216
the ventral dendritic arbor of marginal (lamina i) neurons in the adult primate spinal cord.the ventral (gelatinosal) dendritic arbor of marginal neurons taken from lumbosacral cord was examined in golgi preparations from adult macaque monkey and adult squirrel monkey where neurons were filled with horseradish peroxidase (hrp) via secondary diffusion. based on morphological criteria two types of marginal neurons were found which give rise to a substantial ventral dendritic arbor. these dendrites penetrate the depth of lamina ii and some extend well into lamina iii. the cell bodies are ...1979119186
identification of the pallidal and peripallidal cells projecting to the habenula in monkey.injections of horseradish peroxidase (hrp) into the habenula of squirrel monkeys labeled a multitude of neurons in the lateal hypothalamus and a lesser number of neurons in the internal pallidum (gpi). the distribution of labeled cells is heterogeneous and many hrp-marked pallidal neurons are intermingled with acetylcholinesterase (ache)-rich cells in the internal medullary lamina. however, these ache neurons do not appear to contribute significantly to the innervation of the habenula. these fin ...1979119192
response of peritoneal cells to horseradish peroxidase and aldehyde-fixed erythrocytes in the mouse: an electron microscope adult dd-mice, response of peritoneal free cells to either horseradish peroxidase (hrp) or glutaraldehyde-fixed erythrocytes were examined by electron microscopy. as reported previously (abe et al., 1979), peritoneal free cells are classified into three types: type i, ii and iii cells. type i cells are small lymphocytes, type ii cells are medium-sized mononuclear cells, and type iii cells are macrophages. the amount of hrp taken up is very large in type iii cells. the cells contain numerous v ...1979120163
[ultrastructural demonstration of the phagocytic activity of periodontal ligament fibroblasts in the rat using enzymatic tracers].the ultrastructural study of non-decalcified periodontal ligament of rats, labeled with horseradish peroxidase used as an enzymatic tracer for the mechanism of endocytosis, shows that there exists in the fibroblast, on the one hand, an endogeneous peroxidase revealed by the preparation and characteristic of certain cellular organelles: mitochondria, free ribosomes, ribosomes of the granular endoplasmic reticulum; and on the other hand an exogeneous peroxidase which demonstrates the ability of fi ...1977122704
spontaneous and osmotically-stimulated activity in slices of rat hypothalamus.single unit activity was recorded from 400-500 mu m thick slices of rat hypothalamus, using either nacl- or horseradish peroxidase-filled glass micropipettes. spontaneous activity was present in the following hypothalamic loci: anterior hypothalamic-preoptic area, nucleus circularis, nucleus of the diagonal band of broca, paraventricular accessory nucleus, paraventricular nucleus (all portions), periventricular regions of the anterior hypothalamus, and the suprachiasmatic nucleus. the supraoptic ...1978122715
comparative ultrastructure and blood-brain barrier in diapause and nondiapause larvae of the european corn borer ostrinia nubilalis (hübner).ultrastructural examination of diapause and nondiapause larval brains of the european corn borer disclosed anatomical differences that may be related to the insect's "blood-barrier". the perineurial type i cells are quite closely appressed in the diapause brain, but thrown into extensive folds with large intercellular spaces in the nondiapause brain. the perineurial type ii cells of diapause and nondiapause larvae are basically similar in general ultrastructure, and most likely form the basis fo ...1975126807
[afferent projections to the locus coeruleus nucleus in the cat. study by the horseradish peroxidase technic].using a retrograde tracer technique with horseradish peroxidase, we have revealed some afferent projections to the locus coeruleus complex from the contralateral pontine tegmentum, raphe nuclei, substantia nigra, nucleus of the solitory tract, dorsal motor nucleus of the vagus and other regions of the ponto-bulbar reticular formation as well as from hypothalamic and preoptic areas.1976134760
the ultrastructural histochemistry of the basement membranes of the exocrine pancreas.the basement membranes in the exocrine pancreas were examined by routine electron microscopy, by fixation for electron microscopy with a glutaraldehyde-tannic acid solution and by staining for ultrastructural demonstration of mucosubstances with the dialyzed iron, high iron diamine, ruthenium red and concanavalin a-horseradish peroxidase procedures. the basement membranes are considered from morphologic and histochemical observations to consist of an inner lamina lucida, an intermediate lamina ...1977143223
investigation of the endothelial cell changes in the brain of shr using horseradish peroxidase technique and enzyme histochemistry [proceedings]. 1977143545
the cellular response to topically applied horseradish peroxidase in the gingiva of normal and immunized dogs. i. a histometric and ultrastructural study. 1978147331
early changes in the arterial endothelium under various pathological conditions: an electron histochemical study with visualization of altered permeability using electron microscopic tracers.the authors have studied the endothelial changes in the aorta and the arteries of heart, kidney and other organs in cholesterol fed rabbits and endotoxin administrated rats through electron histochemical and vascular clearance methods. the vascular changes are variable presumably due to the functional and morphological difference of vessels. the administration of cholesterol and endotoxin may result in increased vascular permeability which is directly related to the qualitative increase and decr ...1979155387
placental barrier against circulating immune complexes. a study in pregnant guinea pigs using maternal injection of horseradish peroxidase soluble immune is not rare that the so-called immune complex diseases (some of which were recently supposed to be induced by the biological effects of circulating antigen-antibody immune complexes) are concomitantly present in pregnant females. but there have been very few reports of congenital immune complex diseases.1979157282
[the topographical localization of spinal motoneurons of the rat and its numerical alternation in regard to development (author's transl)].the topographical localization of spinal motoneurons innervating the forelimb muscles of the rat and its numerical alternation in regard to development was observed by hrp (horseradish peroxidase) method. hrp method is a histochemical technique to trace the cellular origin of axon terminal net work. when hrp is injected into the muscles, the enzyme is taken up by nerve endings and transported retrogradely by the axoplamic flow to the original cell body, and does not transported hematogenously an ...1979159327
muscle spindle capillaries and capsule: a horseradish peroxidase study. 1978160105
accelerated immune complex nephritis due to mesangial overloading in spontaneous hypertensive (shr) rats.immune complex nephritis induced by bovine serum albumin (bsa) and horseradish peroxidase (hrp) was superimposed upon spontaneous hypertensive rats (shr rats) which were pretreated with polyvinyl alcohol (pva). pva accumulated predominantly in the mesangium, and the superimposed nephritis developed more accelerated glomerular damage with marked capillary deposition of immune complexes than control animals which were not pretreated with pva.1979161594
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