the reaction of ferrous horseradish peroxidase with hydrogen peroxide. 19705442280
the effects of amino acids and ammonium on the growth of plant cells in suspension culture.a suspension culture of soybean (glycine max l.) was grown on a defined medium in which the nitrogen sources were nitrate (25 mm) and ammonium (2 mm). the cells did not grow on nitrate unless the medium was supplemented with ammonium or glutamine. the l- and d-isomers of 12 amino acids tested singly could not replace ammonium. most amino acids (4 mm) inhibited growth when the cells were cultured on nitrate and ammonium. cells from five other plants (reseda luteoli l.; triticum monococcum l.; fla ...197016657321
immunohistochemistry of glomerulonephritis using horseradish peroxidase and fluorescein-labeled antibody: a comparison of two technics. 19704192221
effects of denaturing agents on the phenylalanyl circular dichroism bands of horseradish peroxidase isoenzymes and apoisoenzymes. 19705436945
thin-layer isoelectric focusing on sephadex layers of horseradish peroxidase. 19705416129
binding of fluorescent probe, 1-anilino-8-naphthalene sulfonate, to apo-horseradish peroxidase. 197011947363
a monophenol oxidase activity in extracts of sorghum.a p-hydroxycinnamic acid oxidase activity was present in enzyme preparations from first internodes of sorghum vulgare variety wheatland milo when incubated in phosphate buffer at ph 7.5. this preparation had no classical polyphenolase activity but had both peroxidase and catalase activities. since horseradish preparations catalyzed the same reaction, the oxidation probably is another example of a peroxidase-oxidase reaction. a second substrate was p-hydroxyphenylpyruvic acid. ferulic acid was sl ...197016657306
uptake of peroxidose by the cockroach central nervous system.the central nervous system of the cockroach has been incubated with solutions of an exogenous tracer substance, horseradish peroxidase, and the sites of its penetration and uptake have been studied by electron microscopy. when the nervous tissue is intact, or intact but stretched, the peroxidase is taken up throughout the neural lamella and also penetrates short distances into the extracellular space between adjacent perineurial cells. when the ganglia have been desheathed, reaction product for ...197018631522
specific antibody within lymphoid germinal center cells of mice after primary immunization with horseradish peroxidase: a light and electron microscopic study.the appearance in mice of specific antibody within newly formed germinal centers in lymph nodes was demonstrated by light and electron microscopy after regional primary antigenic stimulation with horseradish peroxidase (hrp). lymphoid germinal center cells containing anti-hrp antibody in the perinuclear space and in the cytoplasm were detected from 17 to 26 days after antigenic stimulation. extracellular anti-hrp antibody within germinal centers, localized between dendritic reticular cells and l ...19705409949
co as a ligand of photosensitive p-630 transformed from horseradish peroxidase in the presence of indole-3-acetic acid. 19704313768
iaa oxidase inhibitors from normal and mutant maize plants.extracts of maize (zea mays l.) plants contain substances which, in vitro, inhibit an indoleacetic acid (iaa) oxidase enzyme from maize. the extracts can be freed of inhibitors by dialysis or by passage through columns of polyvinylpyrrolidone powder. inhibitor-free extracts contain an iaa oxidase enzyme which requires a phenolic co-factor and is stimulated by mn(2+).iaa oxidase inhibitor and total phenol levels were compared for normal maize and for the maize mutant knotted (kn). in plants up to ...196916657240
mössbauer spectroscopic evidence for the electronic configuration of iron in horseradish peroxidase and its peroxide derivatives. 19695346394
the combination of carbon monoxide-haem with apoperoxidase.1. static titrations reveal an exact stoicheiometry between various haem derivatives and apoperoxidase prepared from one isoenzyme of the horseradish enzyme. 2. carbon monoxide-protohaem reacts rapidly with apoperoxidase and the kinetics can be accounted for by a mechanism already applied to the reaction of carbon monoxide-haem derivatives with apomyoglobin and apohaemoglobin. 3. according to this mechanism a complex is formed first whose combination and dissociation velocity constants are 5x10( ...19695343772
studies on cytochrome c peroxidase. xv. comparison of manganese porphyrin-containing cytochrome c peroxidase, horseradish peroxidase, and myoglobin. 19694309145
the effects of temperature and ionic strength on the formation of the hydroxide complex of ferric horseradish peroxidase. 19695820991
enzymology of ergot alkaloid biosynthesis. iv. additional studies on the oxidation of agroclavine by horseradish peroxidase. 19695356034
compound 3 kinetics and chemiluminescence in oscillatory oxidation reactions catalyzed by horseradish peroxidase. 19694307586
[a test of onion and horseradish leaf phytoncides in bothriocephaliasis of white amur]. 19695392285
the effect of ligand binding and acid splitting on the optical rotatory dispersion of ferric horseradish peroxidase. 19685723400
peroxidase isoenzymes from horseradish roots. 3. circular dichroism of isoenzymes and apoisoenzymes. 19685658539
circular dichroism of horseradish peroxidase isoenzymes and apoisoenzymes. ucla-12-686. 19685732289
transformation of horseradish peroxidase into photosensitive compounds in the presence of indole-3-acetic acid. 19685668185
transverse tubular system in glycerol-treated skeletal muscle.horseradish peroxidase used as an extracellular marker fills 98.5 percent of the central triadic elements (tubules) in normal muscle and 97.2 percent in muscle soaked in ringer solution to which 400-millimolar glycerol is added. a glycerol-soaked muscle rapidly returned to normal ringer solution has a disrupted transverse tubular system and has only 3.2 percent of triads filled with peroxidase.19685648264
the ultrastructural basis of alveolar-capillary membrane permeability to peroxidase used as a tracer.the permeability of the alveolar-capillary membrane to a small molecular weight protein, horseradish peroxidase (hrp), was investigated by means of ultrastructural cytochemistry. mice were injected intravenously with hrp and sacrificed at varying intervals. experiments with intranasally instilled hrp were also carried out. the tissue was fixed in formaldehyde-glutaraldehyde fixative. frozen sections were cut, incubated in graham and karnovsky's medium for demonstrating hrp activity, postfixed in ...196811905208
spectral studies of iron coordination in hemeprotein complexes: difference spectroscopy below 250 order to evaluate the feasibility of observing the spectral behavior of protein groups in the coordination sphere of the iron in hemeproteins, criteria are developed to determine whether or not the application of difference absorption spectroscopy to the study of complex formation will be successful. absolute absorption spectra, 300-1100 mmu, from bacterial catalase complexes are displayed, and the infrared bands correlated with magnetic susceptibility values of similar complexes of other hem ...19685699802
conversion of griseophenone a to (+) or (-)-dehydrogriseofulvin in the presence of horseradish peroxidase and hydrogen peroxide. 19685657554
the electronic structure of protoheme proteins. i. an electron paramagnetic resonance and optical study of horseradish peroxidase and its derivatives. 19685646479
studies on the mechanism of the horseradish peroxidase catalyzed luminescent peroxidation of luminol. 19685648750
the oxidation of horseradish peroxidase by periodate. 19684296876
a comparison of horseradish peroxidase and manganese ions as catalysts for the oxidation of dihydroxyfumaric acid.with horseradish peroxidase as catalyst the main product was dihydroxytartrate, but small amounts of glycolaldehyde, mesoxalic semialdehyde, mesoxalate and possibly glyoxal were also formed. mn(2+) catalysis gave rise only to mesoxalate and oxalate. when oxygen uptake was followed by a manometric method the rate of the peroxidase-catalysed reaction was proportional to oxygen concentration and marked inhibition by cyanide was obtained only at low buffer concentration. the catalytic effects of per ...19685660638
alkylation of horseradish peroxidase with iodoacetate. 19685661605
tetanus toxin: fine structure localization of binding sites in striated muscle.when striated muscle from mice poisoned with purified tetanus neurotoxin was incubated with tetanus antitoxin labeled with horseradish peroxidase and stained for peroxidase activity, an electron-opaque reaction product was found in the transverse and terminal sacs of the longitudinal elements of the sarcotubular system. demonstration of toxin in this location suggests that it acts by interfering with contraction coupling or mechanisms of contraction-relaxation.19685716138
circular dichroism of horseradish peroxidase and its enzyme-substrate compounds. 19685640166
participation of horseradish oxyperoxidase (compound 3) in interenzymic reaction steps. 19685640162
reactions of horseradish peroxidase with azide. evidence for a methionine residue at the active site. 19676073036
biosynthesis of ethylene. ethylene formation from methional by horseradish peroxidase. 19676066254
the columnar cells occurring in the parietal layer of bowman's capsule. cellular fine structure and protein transport.the renal corpuscles of adult, c3h swiss, male mice contain testosterone-sensitive, columnar cells in the parietal layer of bowman's capsule. a study of the normal fine structure of these cells reveals several distinctive characteristics: a microvillous brush border; apical tubular invaginations and apical tubules; an elaborate infolding of the basal surface membrane forming cellular compartments, which contain numerous mitochondria; and a complex group of membrane-limited cytoplasmic inclusions ...19674861776
the ultrastructural basis of capillary permeability studied with peroxidase as a tracer.the transendothelial passage of horseradish peroxidase, injected intravenously into mice, was studied at the ultrastructural level in capillaries of cardiac and skeletal muscle. peroxidase appeared to permeate endothelial intercellular clefts and cell junctions. abnormal peroxidase-induced vascular leakage was excluded. neutral lanthanum tracer gave similar results. the endothelial cell junctions were considered to be maculae occludentes, with gaps of about 40 a in width between the maculae, rat ...19676061717
indoleacetic acid oxidase activity of apoperoxidase.the conventional activity of electrophoretically purified horseradish peroxidase toward guaiacol, pyrogallol, 2,6-dimethoxyphenol, and benzidine is abolished by removal of the heme prosthetic group with a mixture of cold acetone and hydrogen chloride. the apoenzyme, though devoid of peroxidase activity, retains its activity as an indoleacetic acid oxidase when it is supplied with 10(-5) mole of manganous ion and 2,4-dachlorophenol per liter. this oxidase activity is cyanide-sensitive; azide also ...19676038167
modification of enzymic activity. 3. effect of salts and organic additives on horseradish peroxidase. 19676078084
the pathway between hyaloid blood and retinal neurons in the toad. structural observations and permeability to tracer substances.the hyaloid vessels form a capillary network on the inner surface of the retina. these capillaries are embedded in the vitreous humor, and they lack a glial investment. the intercellular spaces of the retina communicate with the ocular cavity, as can be evidenced by following the penetration of tracer substances. hence, there is an extracellular diffusion pathway between hyaloid capillaries and retinal neurons, without interposition of glial cells. trypan blue and ferrocyanide were not detected ...19676035649
studies on the equilibria and kinetics of the reactions of peroxidases with ligands. 3. the dissociation of carbon monoxide from carbon monoxide ferro-horseradish peroxidase. 19676049439
fine structural localization of a blood-brain barrier to exogenous peroxidase.horseradish peroxidase was administered to mice by intravenous injection, and its distribution in cerebral cortex studied with a recently available technique for localizing peroxidase with the electron microscope. brains were fixed by either immersion or vascular perfusion 10-60 min after administration of various doses of peroxidase. exogenous peroxidase was localized in the lumina of blood vessels and in some micropinocytotic vesicles within endothelial cells; none was found beyond the vascula ...19676033532
peroxidase isozymes from horseradish roots. ii. catalytic properties. 19676026238
enzyme-labeled antibodies for the light and electron microscopic localization of tissue antigens.enzymes, either acid phosphatase or horseradish peroxidase, were conjugated to antibodies with bifunctional reagents. the conjugates, enzymatically and immunologically active, were employed in the immunohistochemical localization of tissue antigens utilizing the reaction product of the enzymatic reaction as the marker. tissues reacted with acid phosphatase-labeled antibodies directed against basement membrane were stained for the enzyme with gomori's method, and those reacted with peroxidase-lab ...19676046571
analysis of the conditions causing the oscillatory oxidation of reduced nicotinamide-adenine dinucleotide by horseradish peroxidase. 19674291647
a rapid assay for peroxidase activity.1. peroxidase has been assayed by a chronometric method involving the coupled reaction of ascorbic acid with the product of the enzymic action on benzidine. 2. measurements of the activities of horseradish and tea peroxidase by this and two other methods, involving respectively pyrogallol and o-dianisidine, are compared. 3. it is claimed that the chronometric method is relatively simple, rapid and accurate. 4. the method can be used in the presence of polyphenol oxidases.196616742428
studies on cytochrome c peroxidase. iv. a comparison of peroxide-induced complexes of horseradish and cytochrome c peroxidases. 19665911629
the behavior of horseradish peroxidase at high hydrogen peroxide concentrations. 19665963444
reversible conversion of horseradish paraperoxidase (low spin) into normal peroxidase (high spin) by sulfhydryl reagents. 19665961094
peroxidase isozymes from horseradish roots. i. isolation and physical properties. 19665946638
[contribution to the study of mechanisms of indolylacetic acid degradation by horseradish peroxidase]. 19665940633
the role of horseradish peroxidase in indole-3-acetic acid oxidation. 19655881594
the formation of thyroxine from diiodotyrosine in the presence of horseradish peroxidase and hydrogen peroxide. 19655325809
investigations on the multiple components of commercial horseradish peroxidase. 196514298831
the activity of the horseradish peroxidase compound 3. 196514265755
the hydroxylation of proline by horseradish peroxidase. 196414249129
biosynthesis of mustard oil glucosides. iv. the administration of methionine-c14 and related compounds to horseradish. 196414209394
[electrophoretic and chromatographic separation of peroxidase in horseradish]. 196414318458
[alteration of bacterial toxins by the constituents of chamomile and horseradish. 4]. 196314208688
the stimulation of horseradish peroxidase by nitrogenous ligands. 196314086725
the oxidatic activity of horseradish peroxidase. ii. participation of ferroperoxidase. 196314109214
the oxidatic activity of horseradish peroxidase. i. oxidation of hydro- and naphthohydroquinones. 196314109213
[alteration of bacterial toxins by the ingredients of camomile and horseradish. 1]. 196314117727
biosynthesis of mustard oil glucosides. i. administration of c14-labelled compounds to horseradish, nasturtium, and watercress. 196213995362
[the antibacterial action of ethereal oils from horseradish root (cochlearia armoracia l.)]. 196013755847
[comparative clinical studies on non-specific urinary tract infections with an enzyme-glycoside mixture obtained from horseradish roots (cochlearia armoracia l.)]. 196013747687
[on the factors influencing the reaction of the horseradish peroxidase-ascorbic acid system]. 195913806578
[treatment of diseases of the respiratory tract with an extract from cochlearia armoracia (horseradish)]. 195913643388
[studies of antibacterial substances from horseradish (cochlearia armoracia), nasturtium (tropaeolum maius) and garden peppergrass (lepidium sativum)]. 195713435755
oxidation-reduction potentials of horseradish peroxidase. 195713416301
a simple method for the preparation of horseradish peroxidase. 195413172193
the chemical nature of the second hydrogen peroxide compound formed by cytochrome c peroxidase and horseradish peroxidase. 2. formation and decomposition. 195313093668
the chemical nature of the second hydrogen peroxide compound formed by cytochrome c peroxidase and horseradish peroxidase. i. titration with reducing agents. 195313058869
the third intermediate compound of horseradish peroxidase and hydrogen peroxide. 195313044812
intermediate compounds formed when horseradish peroxidase reacts with potassium molybdicyanide and potassium chloriridate. 195313038479
chemical nature of the secondary hydrogen peroxide compound formed by cytochrome-c peroxidase and horseradish peroxidase. 195214929252
horseradish peroxidase. 195214919613
splitting of horseradish peroxidase into prosthetic group and protein as a means of studying the linkages between hemin and protein. 195214904464
the enzyme-substrate compounds of horseradish peroxidase and peroxides; kinetics of formation and decomposition of the primary and secondary complexes. 194918131422
[not available]. 194720254343
the action of horseradish-peroxidase on angiotonin, pepsitensin and epinephrine.(1) the pressor action of angiotonin, pepsitensin and epinephrine is rapidly destroyed by the horseradish-peroxidase-hydrogen peroxide system. (2) this reaction is considerably enhanced by the addition of small amounts of potassium iodide.194517730622
factors affecting regeneration of the horseradish root. 194016653625
oxidations catalysed by horseradish- and milk-peroxidases. 193216744935
metabolism of carbamazepine in plant roots and endophytic rhizobacteria isolated from phragmites australis.carbamazepine (cbz) is a pharmaceutical frequently categorized as a recalcitrant pollutant in the aquatic environment. endophytic bacteria previously isolated from reed plants have shown the ability to promote growth of their host and to contribute to cbz metabolism. in this work, a horseradish (armoracia rusticana) hairy root (hr) culture has been used as a plant model to study the interactions between roots and endophytic bacteria in response to cbz exposure. hrs could remove up to 5% of the i ...201728823920
production of a conjugate between the rk346 antigen from leishmania infantum and the horseradish peroxidase c for the detection of rk346 was designed and characterized a reporter system to be captured by an- tibodies bound to elisa plates. the system was designed with the rk346 from leishmania infantum, a highly antigenic and specific protein. the rk346 was coupled to the horseradish peroxidase c (hrpc) from armoracia rusticana using glutaraldehyde or sulfo-smcc. gluta- raldehyde conjugation was performed in two steps. separation of conjugates was carried out using a sepharose s-200 in size exclusion chromatography (sec); frac ...201628429896
transient expression and purification of horseradish peroxidase c in nicotiana benthamiana.horseradish peroxidase (hrp) is a commercially important reagent enzyme used in molecular biology and in the diagnostic product industry. it is typically purified from the roots of the horseradish (armoracia rusticana); however, this crop is only available seasonally, yields are variable and often low, and the product is a mixture of isoenzymes. engineering high-level expression in transiently transformed tobacco may offer a solution to these problems. in this study, a synthetic nicotiana bentha ...201829301255
novel characteristics of horseradish peroxidase immobilized onto the polyvinyl alcohol-alginate beads and its methyl orange degradation potential.herein, we report the immobilization of in-house isolated horseradish peroxidase (hrp) from armoracia rusticana with novel characteristics. the hrp was immobilized onto the self-fabricated polyvinyl alcohol-alginate (pva-alginate) beads using sodium nitrate as a cross-linker. the pva-alginate beads (2.0mm size) developed using 10% pva and 1.5% sodium alginate showed maximal immobilization yield. the surface morphologies of the pva-alginate (control) and immobilized-hrp were characterized by scan ...201728712997
unravelling important odorants in horseradish (armoracia rusticana).horseradish (armoracia rusticana) is a plant well known for its roots' spicy aroma. the present study investigates the main aroma constituents of horseradish roots in general by analysing the aroma profiles of six different horseradish varieties, with one variety grown in two different soils. odorants were characterised by means of gas chromatography-olfactometry and identified via their mass spectra, retention indices on two columns with different polarity, and their characteristic odour. a ser ...201728490098
spasmolytic, antimicrobial and cytotoxic activities of 5-phenylpentyl isothiocyanate, a new glucosinolate autolysis product from horseradish (armoracia rusticana p. gaertn., b. mey. & scherb., brassicaceae).detailed analyses of horseradish autolysates led to the identification of a new natural product, 5-phenylpentyl isothiocyanate (phpeitc). the structural assignment was corroborated by synthesis, and the identity unequivocally established by spectral means. the occurrence of phpeitc is the first direct proof of the existence of a 5-phenylpentyl glucosinolate in the aerial parts of this species as one of the possible "mustard oil" precursors. to verify its possible contribution to the horseradish ...201728490082
Displaying items 20601 - 20690 of 20690