| ultrastructural immunoenzymatic study of alpha-fetoprotein-producing cells in the human fetal liver. | cells producing alpha-fetoprotein in human fetal liver have been studied with specific horseradish peroxidase labeled immunoglobulins. under light microscopy, the alpha-fetoprotein is strictly localized in the cytoplasm of certain hepatocytes, distributed randomly in the hepatic lobule. ultrastructural examination of thesame cells shows that the alpha-fetoprotein is present within the cytoplasm. ultrastructural differences are described in hepatocytes according to whether or not the cell is prod ... | 1977 | 69654 |
| axoplasmic transport in ocular hypotony and papilledema in the monkey. | orthograde and retrograde axoplasmic transport were studied in optic nerve heads of seven hypotensive macaca fascicularis eyes. orthograde transport was studied by radioautography after intravitreal radioisotope injections. retrograde transport was studied in the same eyes by horseradish peroxidase injection into the dorsal lateral geniculate nuclei or optic tracts. three eyes had developed marked papilledema before injections. orthograde axoplasmic transport was blocked in swollen axons of the ... | 1977 | 70200 |
| bidirectional movement of horseradish peroxidase and the demonstration of reciprocal thalamocortical connections. | | 1977 | 70257 |
| evidence for direct synaptic interconnections between cat spinal alpha-motoneurons via the recurrent axon collaterals: a morphological study using intracellular injection of horseradish peroxidase. | by using intracellular injection of horseradish peroxidase into cat triceps surae alpha-motoneurons we have been able to trace the motor axons as well as the recurrent motor axon collaterals with their synaptic terminals both with the light and electron microscope. in addition to the expected projection to the 'renshaw cell area', the triceps surae motor axon collaterals were frequently found to terminate within the motor nuclei, where at least some of the terminals made direct synaptic contact ... | 1977 | 70258 |
| role of gangliosides in the uptake and retrograde axonal transport of cholera and tetanus toxin as compared to nerve growth factor and wheat germ agglutinin. | previous investigations have shown that tetanus toxin is transported retrogradely in all peripheral neurons whereas the transport of ngf is confined to adrenergic and sensory neurons. other macromolecules with molecular weights and general physiochemical properties similar to ngf and tetanus toxin (e.g., cytochrome c, insulin, horseradish peroxidase and bovine serum albumin) are not transported to a detectable extent if injected in comparable molar concentrations. for tetanus toxin, which is tra ... | 1977 | 70259 |
| retrograde axonal transport of horseradish peroxidase for determining motor projection patterns to the developing limb in xenopus. | horseradish peroxidase (hrp) injected into developing limb buds of xenopus laevis tadpoles is carried by retrograde axonal transport to the somata of motoneurones in the ventral horn. small injection of 10% hrp were found to remain well localised to specified sites in the limb bud. two types of labelled cells were found: diffusely labelled and granular labelled. diffusely labelled cells result from axonal damage in the presence of hrp. granular labelled cells result only from uptake of hrp from ... | 1977 | 70260 |
| [projections of bulbar reticular nuclei and raphe nuclei to lobules vi and vii of cat cerebellar cortex]. | injections of horseradish peroxidase were made in lobules vi and vii of the cerebellar vermis (in the cat). serial frontal sections of the brain stem reveal that the lateral and paramedian nuclei were essentially projected onto lobule vi, and the projections from the raphe nuclei onto lobule vii. | 1977 | 70281 |
| origin of the direct hippocampus--anterior thalamic bundle in the rat: a combined horseradish peroxidase--golgi analysis. | | 1977 | 71242 |
| localization of thyroiglobulin antigenicity in rat thyroid sections using antibodies labeled with peroxidase or 125i-radioiodine. | in the hope of localizing thyroglobulin within focullar cells of the thyroid gland, antibodies raised against rat thyroglobulin were labeled with the enzyme horseradish peroxidase or with (125)i-radioiodine. sections of rat thyroids fixed in glutaraldehyde and embedded in glycol methacrylate or araldite were placed in contact with the labeled antibodies. the sites of antibody binding were detected by diaminobenzidine staining in the case of peroxidase labeling, and radioautography in the case of ... | 1977 | 71304 |
| movement of horseradish peroxidase in the cornea, sclera and the anterior uvea. | the anterior chambers of rabbit, monkey and human eyes were perfused with horseradish peroxidase. the perfusion was started immediately after enucleation of the monkey and human eyes, while the rabbit eyes were perfused in situ after the animals had been killed. comparative results were obtained after 1 h or perfusion and were mainly based on frozen sections. intensive staining was found in the descemet's membrane, the sclera and the iridial stroma, considerably more than in the corneal stroma a ... | 1977 | 71844 |
| the permeability of the human ciliary and iridial epithelium to horseradish peroxidase. an in vitro study. | the permeability of the human ciliary epithelium to horseradish peroxidase (po) has been studied in vitro with the electron microscope. ciliary body and iris specimens were obtained from freshly enucleated eyes. po was applied at the stromal side of the epithelium, and was left for 120 min. the movement of po through the intercellular spaces of the human ciliary epithelium was blocked apically in the lateral intercellular spaces of the non-pigmented epithelial cells, indicating that these cells ... | 1977 | 71845 |
| arborization of initial axon collaterals of spinocervical tract cells stained intracellularly with horseradish peroxidase. | the initial axon collateral arborizations of two feline spinocervical tract cells have been analysed after intracellular staining with horseradish peroxidase. the images of the cells were reconstructed in the light microscope from transverse serial sections. the cell bodies were situated in laminae iv or iii of the lumbar dorsal horn. each cell exhibited one initial collateral. these emerged from the parent axons at distances of about 310 and 580 micron, respectively, from the cell body. the col ... | 1977 | 71934 |
| the pontine projection to the crebellar anterior lobe. an experimental study in the cat with retrograde transport of horseradish peroxidase. | | 1977 | 72000 |
| detection of epstein-barr virus antigens with enzyme-conjugated antibody. | monospecific conjugated (fluorescein isothiocynate and horseradish peroxidase) goat antisera, prepared against three human immunoglobulin classes, im (mu), igg (gamma) and iga (alpha), were compared for their ability to detect human ig classes possessing specificity for epstein-barr virus (ebv) viral capsid antigens (vca) in a chronically infected human lymphoid cell line, p3j-hrik. it was determined that the enzyme system was significantly more sensitive than immunofluorescence in detecting mos ... | 1977 | 72045 |
| observations on labeling of neuronal cell bodies, axons, and terminals after injection of horseradish peroxidase into rat brain. | | 1977 | 72079 |
| the olivocerebellar projection studied with the method of retrograde axonal transport of horseradish peroxidase. v. the projections to the flocculonodular lobe and the paraflocculus in the rabbit. | hrp was injected in the flocculonodular lobe and the paraflocculus in the rabbit to determine the areas of the inferior olive which project onto these cerebellar regions. following injections in the flocculus labeled cells occurred in the dorsal cap and the rostralmost tip of the medial accessory olive. following injections in the nodulus labeled cells were likewise found in the dorsal cap, but in addition in the rostralmost part of the dorsomedial cell column and the adjoining part of the media ... | 1977 | 72080 |
| the olivocerebellar projection in the cat studied with the method of retrograde axonal transport of horseradish peroxidase. vi. the projection onto longitudinal zones of the paramedian lobule. | microinjections (30-50 nl) of a horseradish peroxidase (hrp) suspension of 25% (wt./vol.) were made in different folia of the paramedian lobule of cats, and the sites of occurrence of labeled cells in the inferior olive were precisely determined. in each case only a small number of cells are labeled, aggregated in a minute area. the labeled cells are found within three only of the four olivary areas previously determined (brodal et al., '75) to project onto the paramedian lobule (fig. 1): one ar ... | 1977 | 72081 |
| salivatory neurons innervate the submandibular and sublingual glands in the rat: horseradish peroxidase study. | | 1977 | 72589 |
| cerebeller afferents from neurons in motor nuclei of cranial nerves demonstrated by retrograde axonal transport of horseradish peroxidase. | | 1977 | 72590 |
| somatopetal transport of horseradish peroxidase (hrp) in the peripheral and central branches of dorsal root ganglion cells. | the axonal transport of hrp in both the peripheral and central branches of dorsal root ganglion cells was studied in rats. for studying axonal transport in the peripheral branch hrp as a dry substance was applied to the peroneal nerve injured either by teasing, by cutting or crushing. after a short survival time (22 h) mainly small spinal ganglion cells of the corresponding segments were labelled, while after a prolonged survival time (70 h) mainly large cells were labelled. these labelling diff ... | 1977 | 72611 |
| afferents to the entorhinal cortex of the rat studied by the method of retrograde transport of horseradish peroxidase. | | 1977 | 72679 |
| intracellular staining of cortical neurons by pressure microinjection of horseradish peroxidase and recovery by core biopsy. | | 1978 | 73474 |
| noradrenaline innervation of the spinal cord studied by the horseradish peroxidase method combined with monoamine oxidase staining. | the origin of the spinal cord noradrenaline (na) has been investigated by means of the horseradish peroxidase (hrp) method, combined with monoamine oxidase staining (glenner) to identify the na neurons. following the injection of hrp to the various levels of rat spinal cord, cervical to sacral cord, a1-3, 5-7 na neuron groups were labeled with hrp. they showed almost the same distribution pattern regardless of difference in the injected segment. labeled na neurons in a6 were concentrated in the ... | 1977 | 74341 |
| the pontine projection to the flocculonodular lobe and the paraflocculus studied by means of retrograde axonal transport of horseradish peroxidase in the rabbit. | the occurrence and distribution of labeled cells in the pontine nuclei were mapped following injections of small amounts of horseradish peroxidase (0.05-0.5 microliter, 50% suspension) in the flocculus, nodulus and the dorsal and ventral paraflocculus in adult albino rabbits. while no labeled cells were found in the pontine nuclei following injections in the nodulus, some were present following injections in the flocculus and a great number following injections in the paraflocculus. the projecti ... | 1977 | 74343 |
| further studies on hybrid cell-surface antigens associated with human chromosome 11. | a new human immunogenetic cell-surface activity associated with human chromosome 11 in the al human-chinese hamster ovary cell hybrid is described. like a1, but not a2, it is present on the human erythrocyte. by mutagenesis and selection, specific, stable, variants of the al hybrid have been prepared exhibiting various combinations of a1, a2, a3, and lactic dehydrogenase a activities. the antigens of the al system can be demonstrated by the horseradish peroxidase system which offers a promising ... | 1977 | 74861 |
| techniques for histological reconstruction of brain stem recording sites. | the masseter muscle of sprague-dawley rats was injected with 10 mg of horseradish peroxidase. the electrical activity of the trigeminal motor nucleus was investigated 24-36 hours later using micropipettes filled with a 2.0 m nacl-7% fast green fcf solution. a parallel series of penetrations at 200 micron intervals was made through the motor complex at known depths. the grid formed from these penetrations was reconstructed using the following techniques. marks were made at known depths in one or ... | 1977 | 74873 |
| neurons in and near nucleus caudalis with long ascending projection axons demonstrated by retrograde labeling with horseradish peroxidase. | | 1978 | 75038 |
| horseradish peroxidase as a retrogradely-transported, detailed dendritic marker. | a method is described that produces detailed 'golgi-like' staining in neurons following retrograde transport of horseradish peroxidase (hrp). of 4 commercially available hrp preparations, which were compared for their ability to produce solid neuronal staining in certain regions of the rabbit thalamus 48 h after striatal injection, two preparations (miles laboratories and serva biochemical) were found to be most efficient. when dimethylsulfoxide (dmso) was used as the solvent for the injected hr ... | 1978 | 75041 |
| primary vestibular afferent projections to the cerebellum as demonstrated by retrograde axonal transport of horseradish peroxidase. | | 1978 | 75045 |
| location of single neurons containing hrp for electron microscopy. | a technique is described for finding single neurons containing transported horseradish peroxidase (hrp) for study in the electron microscope. tissue chopper sections which have been reacted to demonstrate hrp are cleared in glycerol and examined with darkfield light microscopy. the granular reaction product is clearly seen, enabling accurate drawings to be made for later dissection for ultramicrotomy. the neuron sought can then be found within a few microns of the cutting face of the block. whil ... | 1977 | 75048 |
| vagal preganglionic innervation of the cat heart--an attempt to identify the medullary "heart area" by the retrograde axoplasmic transport of horseradish peroxidase. | the medullary "heart area", origins of the vagal preganglionic fibers innervating the heart, were identified in 19 cats with the use of horseradish peroxidase (hrp) as a retrograde axonal tracer. when hrp was injected in the a-v node area, s-a node area, right atrial wall and right ventricular wall, the labeled neurons were principally found in the lateral half of the dorsal motor nucleus (dmn) and in the medial solitary nucleus (msn), were almost the same in number bilaterally, and were chiefly ... | 1977 | 75272 |
| electron microscopical study of retrograde axonal transport of horseradish peroxidase in the supraoptico-hypophyseal tract in rat. | | 1978 | 75882 |
| [vascularization of the ganglion spirale cochleae of the rat (author's transl)]. | investigation into the vascularization of the ganglion spirale of the rat showed an irregular, loose network of capillaries, with a somewhat denser vacularization of the marginal areas of the ganglion; the horseradish peroxidase benzidine test according to sugar was used to demonstrate the vessels. at least of the blood supply of the ganglion is effected via the vessels which run parallel to the nerve fibres through the osseous canals from the modiolus into the rosenthal canal. the density of va ... | 1978 | 76371 |
| modification of the horseradish peroxidase method avoiding fixation. | | 1978 | 76495 |
| thalamic afferents to the limbic cortex in the cat studied with the method of retrograde axonal transport of horseradish peroxidase. | thalamic afferents to the limbic cortex in the cat were studied with the method of retrograde axonal transport of horseradish peroxidase. the anterior limbic region receives fibers largely from the anteromedial nucleus and partly from the anterodorsal and anteroventral nuclei. there appears to be a dorsoventral organization of cortical projections of the anteromedial nucleus to the anterior limbic region. the cingular area has its main input from the anteroventral and anteromedial nuclei. the lo ... | 1978 | 76501 |
| cerebellar afferent projections from the perihypoglossal nuclei: an experimental study with the method of retrograde axonal transport of horseradish peroxidase. | details of cerebellar afferent projections from the perihypoglossal nuclei were studied in the cat by means of retrograde axonal transport of horseradish peroxidase (hrp). labeled cells were observed bilaterally (with a preponderance ipsilaterally) in nuclei intercalatus and praepositus hypoglossi following injections in various folia of the entire vermis, paraflocculus, flocculus, fastigial nucleus, and the nucleus interpositus anterior and posterior. relatively high densities of labeled cells ... | 1978 | 76569 |
| commissural interneurons for masticatory motoneurons: a light and electron microscope study using the horseradish peroxidase tracer technique. | | 1978 | 76570 |
| mesencephalic and diencephalic afferents to the superior colliculus and periaqueductal gray substance demonstrated by retrograde axonal transport of horseradish peroxidase in the cat. | the mesencephalic and diencephalic afferent connections to the superior colliculus and the central gray substance in the cat were examined by means of the retrograde transport of horseradish peroxidase (hrp). after deep collicular injections numerous labeled cells were consistently found in the parabigeminal nucleus, the mesencephalic reticular formation, substantia nigra pars reticulata, the nucleus of posterior commissure, the pretectal area, zona incerta, and the ventral nucleus of the latera ... | 1978 | 77176 |
| a morphological study of the axons and recurrent axon collaterals of cat sciatic alpha-motoneurons after intracellular staining with horseradish peroxidase. | utilizing the centrifugal neuronal transport of intracellularly injected horseradish peroxidase (hrp), we have performed a light microscopic (lm) investigation of the intramedullary parts of the axons and axon collaterals of sciatic alpha-motoneurons in the adult cat. the intramedullary parts of the alpha-motor axons had comparatively short internodes (down to 75 microns) and were thinner than reported in earlier studies on the ventral root. positive correlations were obtained when relating noda ... | 1978 | 77281 |
| [study of the neurons of the supraspinal systems of the cat brain using the method of retrograde axonal transport of horseradish peroxidase]. | horseradish peroxidase (hrp) was injected into cervical, thoracic, lumbar and sacral segments of 18 cats. in all the cases hrp was transported retrogradely to the brain stem neurons in the medial reticular formation, vestibular complex and the red nucleus. the present findings demonstrate that, in addition to the above mentioned groups, there exist several other groups of neurons. many labelled neurons are present in the locus coeruleus and the nucleus subcoeruleus, ipsilaterally. these neurons ... | 1978 | 77483 |
| [study of the pathways of the cat spinal cord using the method of experimental retrograde axon transport of horseradish peroxidase]. | funicular trajectories of descending brain stem pathways in the cat were identified by means of retrograde horseradish peroxidase (hpr) transport. in sixteen cats th1 hrp injections preceded by a large c6-c7 lesion sparing the ventral or ventrolateral funiculi and by such a lesion sparing the dorsolateral funiculus were made. it was found that the descending fibres from the nucleus locus coeruleus and the nucleus subcoeruleus are distributed ipsilaterally through the ventrolateral funiculus. the ... | 1978 | 77484 |
| [organization of the sacral spinal cord in relation to the parasympathetic preganglionic neurons and those innervating the perineal sphincteric striated muscles--experimental study in the cat with the use of retrograde axoplasmic transport of horseradish peroxidase (author's transl)]. | | 1978 | 77671 |
| differential projections of neurons within the dorsal raphe nucleus of the rat: a horseradish peroxidase (hrp) study. | | 1978 | 77700 |
| horseradish peroxidase labeling of cat tectotectal cells. | by means of unilateral intracollicular injections of horseradish peroxidase (hrp), the cells of origin of the tectotectal projection in cats were detected in the opposite colliculus. the 9 injected animals were divided into two groups in accordance to the location of their injections along the collicular rostrocaudal axis. the majority of the labeled cells were obtained from two injections performed at the level of the commissure of the superior colliculus (231 cells) whereas 5 injections perfor ... | 1978 | 77703 |
| the pontocerebellar projection of the uvula in the cat. | the occurrence of retrogradely labeled cells in the pontine nuclei was mapped following injections of 0.3-0.4 microliter of a horseradish peroxidase suspension (50% weight/volume) into the uvula (lob. ix of larsell) in the cat. the uvula was found to receive afferents from three pontine cell collections. one of these is situated in the paramedian pontine nucleus close to the midline. it forms a fairly distinctly outlined longitudinal column of cells and is present at all levels of the pons excep ... | 1978 | 77792 |
| subcortical afferents to the prefrontal cortex in rabbits. | the origins of cells projecting to the prefrontal cortex of the rabbit were studied, using horseradish peroxidase (hrp) technique. hrp injected into the prefrontal cortex labeled cells in the basal forebrain, lateral hypothalamus, raphe nuclei and locus coeruleus area on both sides. labeled cells appeared also in the nucleus medialis dorsalis of the thalamus and other thalamic nuclei on the injection side. | 1978 | 77793 |
| uptake and retrograde axonal transport of horseradish peroxidase in regenerating facial motor neurons of the mouse. | uptake and retrograde axonal transport of intravenously injected horseradish peroxidase (hrp) was studied during regeneration after a crush injury of the facial nerve of the mouse. the circulation time of hrp was 12 to 24 h. hrp injected immediately after the crush diffused into injured axons in the crushed region and accumulated subsequently in perikarya of facial neurons in the brain stem. after a time interval of 1 h or 5 days between the crush and the injection only a faint hrp accumulation ... | 1978 | 77897 |
| a neuroanatomical search for glossopharyngeal efferents to the carotid body using the retrograde transport of horseradish peroxidase. | | 1978 | 78741 |
| edinger-westphal nucleus: projections to the brain stem and spinal cord in the cat. | the efferent connections of the edinger-westphal (ew) nucleus of the cat have been examined using the autoradiographic anterograde axonal transport technique. following injections of [3h]amino acids into the ew nucleus, fibers could be traced from this region to a number of sites in the caudal brain stem and spinal cord heretofore not known to receive an afferent input from this nucleus. two descending pathways have been identified. one pathway travels in the medialmost aspect to the medial long ... | 1978 | 78743 |
| anterograde transport of horseradish peroxidase enhanced by poly-l-ornithine. | | 1978 | 78745 |
| [histofluorescence of catecholamines and visualization of retrograde transported peroxidase in the same tissue section and in the same neuron]. | the demonstration of fluorescent catecholamines and horseradish peroxidase (hrp) in the same neuron has been achieved in the rat in two ways: by submitting vibratome sections to a modified glyoxylic acid fluorescence method followed by the usual procedure to reveal hrp; or by combining the last procedure with the cryostat technique of chiba et coll. after hrp injection into the striatum or the nucleus accumbens of the rat, non-fluorescent hrp labelled neurons were observed in the substantia nigr ... | 1978 | 78774 |
| descending course of primary afferent collaterals in the cat's spinal cord. | after injecting horseradish-peroxidase into the lower thoracic, lumbar and sacral spinal cord segments of cats, labelled perikarya were found in several spinal ganglia localized cranially to the site of injection. the segmental distance between the injection site and the rostralmost localized ganglion with labelled cells varied depending on the medio-lateral localization of the injection. the longest distance (10 segments) was achieved by medial injections. primary sensory neurones with long des ... | 1977 | 79302 |
| the projection from nucleus reticularis tegmenti pontis onto the cerebellum in the cat. a study using the methods of anterograde degeneration and retrograde axonal transport of horseradish peroxidase. | | 1978 | 79314 |
| an in vitro method for studying the retrograde intra-axonal transport of horseradish peroxidase in sympathetic neurons. | | 1978 | 79433 |
| ultrastructural identification of dorsal root primary afferent terminals after anterograde filling with horseradish peroxidase. | | 1978 | 79439 |
| afferent fiber connections from the lower brain stem to the rat cerebellum by the horseradish peroxidase method combined with mao staining, with special reference to noradrenergic neurons. | the origin of cerebellar noradrenaline (na) was investigated by means of the horseradish peroxidase (hrp) method, combined with the monoamine oxidase (mao) staining method to identify the na neurons. the present study gives clear cut evidence that the main source for cerebellar na in the rat is the locus coeruleus (lc), both the dorsal and ventral parts which innervate the entire cerebral cortex and hypothalamus respectively. the present study clearly demonstrates the pontine na neurons such as ... | 1978 | 79602 |
| commissural, perihypoglossal and reticular afferent projections to the vestibular nuclei in the cat. an experimental anatomical study with the method of the retrograde transport of horseradish peroxidase. | | 1978 | 80203 |
| the islands of calleja: organization and connections. | the islands of calleja (ic) in the rate are composed of seven small groups of granule cells in the polymorph layer of the olfactory tubercle and one large group, the insula magna, which lies along the border between septum, nucleus accumbens and nucleus of the diagonal band. the cytoarchitecture and neuronal morphology of the ic and surrounding cells, studied using nissl-stained and golgi-kopsch material, are described. in addition, the afferent and efferent connections of the ic were analyzed u ... | 1978 | 80412 |
| uptake and retrograde axonal transport of various exogenous macromolecules in normal and crushed hypoglossal nerves. | macromolecular tracers were injected into the tongue or around a crush in mouse hypoglossal nerves. at various times thereafter, the tracers were histochemically localized on the basis of peroxidase activity. the distribution of reaction product was then examined using light microscopy in order to study the influence of molecular charge and size on uptake and retrograde axonal transport from the periphery or from the crushed axon. of various proteins with peroxidase activity, horseradish peroxid ... | 1978 | 81088 |
| localization of horseradish peroxidase by in situ perfusion with substrate mixture. | | 1978 | 81095 |
| [demonstration of the neurosecretory hypothalamo-rhombencephalic junction in rats by the retrograde axonal transport of horseradish peroxidase]. | the retrograde transport of horseradish peroxidase (hrp) was used to demonstrate the neurosecretory hypothalamo-hindbrain connection of the rat. following hrp injections into the region of the dorsal columns nuclei labeled cells were observed in the caudal part of the paraventricular nucleus and in the lateral hypothalmic area. hypothalamo-hindbrain projections are predominantly uncrossed. | 1978 | 81578 |
| cerebellar afferents from the paramedian reticular nucleus studied with retrograde transport of horseradish peroxidase. | details in the cerebellar projections from the paramedian reticular nucleus (prn) were studied in cats and monkeys by means of retrograde axonal transport of horseradish peroxidase (hrp). in the cat the majority of the fibres projects to the anterior lobe and to the vermis of the posterior lobe (with the exception of lobules viib and viiia). a less conspicuous projection was found to the lobulus simplex, the crura and the flocculus. the cerebellar nuclei, the paramedian lobule and the parafloccu ... | 1978 | 81628 |
| motor innervation of the urinary tract studied by retrograde axonal transport of protein. | a new method for tracing the nervous control of the bladder is described. a protein substance, horseradish peroxidase, has been shown to be absorbed and transported by nerve fibers. the substance was injected into the bladder neck region of a series of dogs. after uptake by motor neurons the horseradish was carried to the spinal cord segment controlling micturition. implications regarding the neurophysiology of the urinary tract are discussed. | 1978 | 81813 |
| the cerebellar projection from locus coeruleus as studied with retrograde transport of horseradish peroxidase in the cat. | the cerebellar afferent projection from locus coeruleus has been studied in the cat by means of retrograde axonal transport of horseradish peroxidase. labelled cells are present bilaterally in locus coeruleus only following injections in the cerebellar vermis (especially its anterior and posterior parts), the ventral paraflocculus and the flocculus. the labelled cells are restricted to the caudal half of the nucleus. a few labelled cells are also present in locus coeruleus following injections i ... | 1978 | 83115 |
| afferent and efferent connections of the parabigeminal nucleus in cat revealed by retrograde axonal transport of horseradish peroxidase. | afferent and efferent connections of the parabigeminal nucleus (pbg) of the cat have been demonstrated by means of horseradish peroxidase (hrp) tracing technique. following hrp injection in the pbg, labelled cells were observed mainly in the deep layers of the ipsilateral superior colliculus (sc). the other labelled structures were the prepositus hypoglossi complex (ph), the ventral nucleus of the lateral geniculate body (lgv), the locus coeruleus, the cuneiform nucleus, the periaqueductal gray ... | 1979 | 83182 |
| electron microscopic study of the orthograde axonal transport of horseradish peroxidase in the supraoptico-neurohypophysial tract of the rat. | horseradish peroxidase (hrp) has been used as a protein tracer in order to visualize the ultrastructural sites of the orthograde transport of protein macromolecules in the hypothalamo-neurohypophysial tract of the rat. after a local injection of hrp within the supraoptic nucleus, the reaction product was observed: (1) mainly in tubules of the smooth endoplasmic reticulum in the more proximal part of the axons, and (2) in granules and microvesicles of the axon terminals. observations on thick sec ... | 1978 | 83193 |
| anatomical and functional aspects of the septo-hippocampal projections. | the origins, distribution, and cellular targets of the septo-hippocampal projections are reviewed. it appears that the distribution of acetylcholinesterase-positive neurons in the medial septum and diagonal bands and those cells labelled after injections of horseradish peroxidase into the hippocampus coincide; however, the possibility of a non-acetylcholinesterase septal projection remains. good agreement is found between the distribution of hippocampal acetylcholinesterase and the patterning of ... | 1977 | 83225 |
| ascending tracts of the lateral columns of the rat spinal cord: a study using the silver impregnation and horseradish peroxidase techniques. | | 1978 | 83245 |
| the cells of origin of the commissural afferents to the area dentata in the mouse. | the hippocampal commissural projection to the area dentata of the mouse was studied using the retrograde horseradish peroxidase (hrp) technique. small volumes of hrp injected into the molecular layer of the fascia dentata or various subareas of regio inferior of the hippocampus (fields ca3a-c) resulted inlabeled perikarya in the contralateral hippocampus and area dentata. the commissural projection to the fascia dentata was observed to originate exclusively from cells within the hilus fasciae de ... | 1979 | 83896 |
| horseradish peroxidase histochemistry combined with glyoxylic acid-induced fluorescence used to identify brain stem catecholaminergic neurons which project to the chick thoracic spinal cord. | | 1979 | 83898 |
| the location of spinal neurons with long descending axons (long descending propriospinal tract neurons) in the cat: a study with the horseradish peroxidase technique. | the distribution spinal neurons with long descending axons was studied in the cat by means of retrograde transport of horseradish peroxidase. labeled neurons appeared bilaterally in the cervical and the thoracic cord following injections in the lumbosacral cord. in some cases hemisections were made rostrally and contralaterally to the injections in an attempt to determine whether or not the axons crossed. neurons with uncrossed descending axons were located in laminae i, v, vii and viii. lamina ... | 1979 | 84003 |
| anatomical organization of the spinocerebellar system in the cat, as studied by retrograde transport of horseradish peroxidase. | the distribution of spinocerebellar tract (sct) neurons has been studied in the entire length of the spinal cord of the cat following injections of horseradish peroxidase into the cerebellum, and whether or not the axons of the labeled neurons crossed within the spinal cord was determined in cases with injections preceded by hemisections at the cervical levels. the scts were classified into the following crossed and uncrossed tracts according to the cell origin and the fiber course; the crossed ... | 1979 | 84004 |
| some immunohistochemical experiments aiming at the electron-microscopic in situ identification of a dental plaque microorganism--streptococcus mutans. | the aim of the present investigation was to test a procedure useful for the electron-microscopic in situ identification of the presumptive cariogenic microorganism streptococcus mutans (serotype d) growing in the human dental plaque. for this purpose, different parameters of an indirect immunohistochemical method were tested in three sets of experiments. in experiment set i, all serological and histochemical procedures were performed en bloc on specimens fixed only with glutaraldehyde before emb ... | 1978 | 84515 |
| the olivocerebellar projections to the flocculus and paraflocculus in the cat, compared to those in the rabbit. a study using horseradish peroxidase as a tracer. | the projections from the inferior olive to the flocculus and paraflocculus in the cat have been mapped by means of the method of retrograde axonal transport of horseradish peroxidase. the findings show that the afferents to the flocculus are derived from the dorsal cap, ventrolateral outgrowth, the principal olive (the caudal parts of the ventral and dorsal lamella) and from the rostral part of the medial accessory olive. the fibres to the paraflocculus come from the caudal part of the principal ... | 1979 | 84697 |
| types of thalamo-cortical relay neurons in the anteroventral nucleus of the cat. a combined horseradish peroxidase--golgi study. | neurons displaying a thalamo-cortical projection were marked by means of the retrograde transport of horseradish peroxidase (hrp), and the labeled elements were compared with neurons impregnated by the golgi technique. injections of hrp into the posterior area of the limbic cortex resulted in its uptake by various anterior thalamic nuclei, especially the anteroventral nucleus. hrp-positive cells are characterized by their position, dendritic orientation, and the shape and size of their somata. o ... | 1979 | 84713 |
| anatomical and functional aspects of the associative projections from somatic area si to sii. | 1. electrophysiological and morphological (retrograde axonal transport of horseradish peroxidase, hrp) experiments have been carried out in the cat in order to study the associative projections from area si to ipsilateral sii. 2. microelectrode recordings were performed in the forepaw focus of sii both in normal (64 units) and in si-undercut (51 units) cats. 29.6% of the neurons recorded in the unoperated and 29.4% of those collected in the operated cats were excited by electric stimulation of t ... | 1979 | 84762 |
| differences in mouse ovarian cells as distinguished by horseradish peroxidase labelling. | the extracellular space of mouse ovarian follicles and stroma contained horseradish peroxidase (hrp) reaction product one minute after intravenous injection of the tracer. in addition to pinocytotic uptake of the tracer, non-vesicular staining with hrp reaction product, heretofore unrecognized, was noted in a variety of ovarian cell-types. this diffuse staining was correlated with changes in cellular morphology suggestive of degeneration. these findings are discussed in relation to the compositi ... | 1979 | 85420 |
| dorsal column nuclei and ascending spinal afferents in macaques. | cell populations and thalamic projections of the dorsal column nuclei in macaques have been investigated in the medullae of normal animals and of animals with injections of horseradish peroxidase in the nucleus ventralis posterolateralis. in the same species, the course, distribution and origin of ascending non-primary pathways to the dorsal column nuclei have been demonstrated with the aid of degeneration methods, 3h-amino acid autoradiography and retrograde axonal transport of horseradish pero ... | 1979 | 85470 |
| neurons in marginal layer of trigeminal nucleus caudalis projecting to ventrobasal complex (vg) and posterior nuclear group (po) demonstrated by retrograde labeling with horseradish peroxidase. | | 1979 | 85475 |
| tectopontine pathway in the cat: laminar distribution of cells of origin and visual properties of target cells in dorsolateral pontine nucleus. | 1. the superior colliculus projects to the dorsolateral nucleus of the pons. retrograde transport of horseradish peroxidase (hrp) revealed that cells in the superior colliculus, which send their axons to the pons, lie in both superficial (iii) and deep (iv--vii) layers. superficial cells outnumbered deep cells. the inferior colliculus also projects heavily to the dorsolateral pontine nucleus. 2. dorsolateral pontine visual cells were activated only by visual stimulation. cells responsive to soma ... | 1979 | 85698 |
| localization of aortic cells in the nodose ganglion by hrp retrograde transport in the cat. | localization of the aortic cells in the nodose ganglion was attempted in the cat using retrograde axonal transport of horseradish peroxidase (hrp). following dissection of the aortic nerve from the vago-aortic trunk, the cut ends of the aortic fibers were immersed in a hrp solution for 23--46 h. labelled cells were found in the nodose ganglion and were located mainly in the medial border of the ganglion. | 1979 | 86177 |
| [distribution pattern in the guinea pig cochlea of intravitally injected peroxidase]. | following perilymphatic perfusion and injection into the cisterna cerebello-medullaris, respectively, the distribution pattern of horseradish peroxidase in the cochlea of the guinea pig was studied light and electron microscopically. the findings prove effective tight junctions (zonulae occludentes) between the cells of the epithelial lining of the endolymphatic compartment. at the level of the reticular membrane the tight junctions are far more extended than elsewhere at the cochlear duct epith ... | 1978 | 86244 |
| an immunoperoxidase assay for serum ragweed-specific ige. | a solid-phase enzyme immunoassay for allergen-specific ige antibodies in serum is descirbed. in this technique these antibodies are allowed to bind to the allergen previously adsorbed to the wells of polystyrene microtiter plates. after a washing step the tubes are incubated with rabbit antihuman ige labelled with horseradish peroxidase. following a second washing step, the enzyme bound to the tubes is assayed spectrophotometrically using o-phenylene diamine as the substrate. the standard curves ... | 1979 | 86310 |
| various types of corticotectal neurons of cats as demonstrated by means of retrograde axonal transport of horseradish peroxidase. | the retrograde labeling of cortical neurons with horseradish peroxidase (hrp) was used to investigate the morphological features of neurons in various cortical areas projecting to the superior colliculus in the cat. corticotectal cells were found to be labeled in layer v of the entire cerebral cortex. the number of labeled cells and their locations varied according to the sites of injections of hrp in the colliculus. most of the corticotectal cells identified in the present study were small (9-- ... | 1979 | 86453 |
| cortical neurons projecting to the pontine nuclei in the cat. an experimental study with the horseradish peroxidase technique. | horseradish peroxidase (hrp) injections in various portions of the cat pontine nuclei resulted in retrograde labeling of neurons in layer v of the ipsilateral cerebral cortex. corticopontine neurons, pyramidal in type, have been found to be labeled in the entire cortex, confirming the previous findings of anterograde degeneration studies. most (91%) of the labeled cells were 14--26 micrometer in diameter (mean 19.4 +/- 4.5 micrometer sd). small (10--20 micrometer) and medium (20--40 micrometer) ... | 1979 | 86454 |
| the anatomical substrate of callosal messages from si and sii in the cat. | horseradish peroxidase (hrp) was injected into the first (si) or second (sii) somatosensory areas of 21 adult cats. the radial and tangential (normal and parallel to the pial surface, respectively) distribution and morphology of the callosal neurons were studied. hrp injections were combined with single unit recording in the contralateral cortex in order to determine which part of the somatosensory periphery is represented within the regions containing callosal neurons, the callosal (efferent) z ... | 1979 | 86455 |
| identification and distribution of the spinal and hypophyseal projection neurons in the paraventricular nucleus of the rat. a light and electron microscopic study with the horseradish peroxidase method. | the distribution of labeled neurons in the paraventricular nucleus of the hypothalamus (pvn) was studied following injections of horseradish peroxidase (hrp) into the spinal cord (c8 to t1) or the hypophysis in the rat. injections were also made in the spinal cord in another group of animals, which were subjected to water deprivation for a period of 3 days, and the pvn of these animals was examined with the electron microscope. spinal projection neurons (paraventriculospinal tract, pvst, neurons ... | 1979 | 86456 |
| tissular immunoenzymatic detection of hepatic alphafetoprotein in human hepatomas. | 5 human cases of hepatoma have been chosen with respect to their different seric alpha-fetoprotein (alpha-fp) level and histological characters. cells producing alpha-fp have been studied with specific horseradish-peroxidase-labelled immunoglobulins. ultrastructural examination shows that alpha-fp is present in the cytoplasm of some tumoral hepatocytes. alpha-fp is also present in the cytoplasm of some rare nontumoral hepatocytes of a nonsecreting hepatoma. ultrastructural differences are descri ... | 1978 | 86473 |
| observations of hrp labeling following injection through a chronically implanted cannula--a method to avoid diffusion of hrp into injured fibers. | one of the limitations of the horseradish peroxidase (hrp) tracer method is the diffusion of hrp into injured axons resulting in unintended labeling of neurons not terminating in the injection area. to overcome this limitation, an experiment was designed to inject the hrp through an implanted cannula after degeneration and healing had taken place. it was shown that implantation of a cannula into the internal capsule significantly decreased the number of labeled axons in the injection site, thus ... | 1979 | 87243 |
| horseradish peroxidase-labelled cells in cervical sympathetic and trigeminal nervous systems after injection of hrp into tooth pulp in monkeys and tree shrews (a preliminary report). | the neurons that project to the tooth pulp were examined by the hrp-retrograde transport technique, using 4 monkeys (3 japanese monkeys and a crab-eating monkey) and 6 tree shrews. the present investigation showed that a large number of sympathetic cervical ganglion neurons, small and medium in size, project to the molar teeth from the superior, middle and inferior ganglia and a small number of gasserian gangilon neurons (small in size) to the molar teeth ipsilaterally. | 1979 | 87281 |
| intracellular marking with lucifer yellow ch and horseradish peroxidase of cells electrophysiologically characterized as glia in the cerebral cortex of the cat. | intracellular microelectrodes filled with either lucifer yellow ch, a highly florescent dye, or horseradish peroxidase (hrp) were used to electrophysiologically characterize and mark cells in the cerebral cortex of cat. fifty-eight cells, characterized electrophysiologically as glia, were marked with lucifer yellow ch. all were identified as protoplasmic astrocytes, and included cells in the glia limitans of the molecular layer. an additional 54 cells, similarly characterized as glia, were label ... | 1979 | 87405 |
| trajectory of group ia afferent fibers stained with horseradish peroxidase in the lumbosacral spinal cord of the cat: three dimensional reconstructions from serial sections. | a reconstruction was made of the intramedullary trajectory of 23 physiologically identified ia afferents from cat hind limb muscles (medial gastrocnemius, soleus, plantaris, flexor digitorum-hallucis longus, and hamstring). the afferents were stained by intra-axonally injected hrp. the axons of these afferents were traced over distances of 5.8 mm to 15.7 mm rostrocaudally. in the dorsal funiculus fibers from all the muscles showed a similar course and similarly bifurcated into an ascending and a ... | 1979 | 87406 |
| the cerebellar projection from the lateral reticular nucleus as studied with retrograde transport of horseradish peroxidase. | the cerebellar projection from the lateral reticular nucleus (nrl) was studied in cats by means of retrograde axonal transport of horseradish peroxidase (the projection to the paramedian lobule was not included, see brodal, 1975, for afferents to this cortical region). the entire cerebellar cortex and all cerebellar nuclei receive fibres from the nrl. the strongest connection is with the anterior lobe and lobulus viiib of the posterior lobe vermis. as concerns the anterior lobe the observations ... | 1979 | 88191 |
| spinal ganglion cells innervating the stomach of the rat as demonstrated by somatopetal transport of horseradish peroxidase (hrp). | spinal ganglion cells innervating the stomach of the rat were demonstrated using the somatopetal horseradish peroxidase (hrp) transport technique. after injection of the tracer into the anterior wall of the stomach, labelled neurons were observed bilaterally within spinal ganglia t4--l1. they were most numerous in ganglia t8--t10. no clear evidence of somatotopic organization of the labelled cells was found. most of the cells measured 35--40 micrometer in diameter. there was a striking paucity o ... | 1979 | 88192 |
| types of striatonigral neurons labeled by retrograde transport of horseradish peroxidase. | | 1979 | 88205 |
| spino-olivary neurones in the lumbo-sacral cord of the cat demonstrated by retrograde transport of horseradish peroxidase. | | 1979 | 88247 |
| electron microscopic identification of mammillary body terminals in the rat's av thalamic nucleus by means of anterograde transport of hrp. a quantitative comparison with the em degeneration and em autoradiographic techniques. | a comparison has been made between the number of horseradish peroxidase (hrp)-labeled mammillary body terminals in the antero-ventral (av) nucleus and the number of such terminals labeled with the electron microscopic (em) autoradiographic [6] and em degeneration technique [6]. with the antero-grade hrp technique less terminals were labeled than with the autoradiographic technique, but more than with the degeneration technique. furthermore it may be concluded that the anterograde hrp technique i ... | 1979 | 88693 |
| transganglionic transport of horseradish peroxidase in primary sensory neurons. | the cut end of the sciatic nerve of adult rats was exposed to horseradish peroxidase (hrp). the rats were allowed to survive for 12 h--5 days. 12--18 h postoperatively small dorsal root ganglion cells more heavily labeled than large ones. after 48--72 h more large cells were intensely labeled. from 18 h onwards large amounts of hrp labeling was observed ipsilaterally in the superficial laminae of the dorsal horn, from 48 h also in deeper laminae and in the gracile nucleus. the heavy labeling cle ... | 1979 | 88696 |
| observations on the morphology of intracellularly stained gamma-motoneurons in relation to their axon conduction velocity. | hindlimb gamma-motoneurons of adult cats were stained intracellularly with horseradish peroxidase. the gamma-motor-axons had intramedullary diameters between 2.0 micron and 2.4 micron and lacked recurrent collaterals. the conduction velocity of the gamma-motor-axons (20-29 m/sec) was close to what could be predicted from the relationship between conduction velocity and intramedullary diameter of much thicker adult alpha-motor-axons. however, the gamma-motor-axons were conducting much faster than ... | 1979 | 88701 |
| visualization of the hrp reaction product using the polarization microscope. | the reaction product of horseradish peroxidase (hrp) staining can be visualized with considerable contrast using the polarization microscope. this is illustrated on whole mount preparations of the cat retina which were stained after injection of hrp into the superior colliculus. when the specimen is placed between two crossed polarizing filters, the stained cells appear rather bright, in contrast to the other components of the tissue which remain dark. the visualization with the polarization mic ... | 1979 | 88702 |