| the arabidopsis functional homolog of the p34cdc2 protein kinase. | the p34cdc2 protein kinase is a key component of the eukaryotic cell cycle, which is required for g1 to s-phase transition and for entry into mitosis. using a 380-base pair dna fragment obtained by polymerase chain reaction amplification from an arabidopsis thaliana flower cdna library as a probe, we isolated and sequenced a cdc2-homologous cdna from arabidopsis. the encoded polypeptide has extensive homology with cdc2-like kinases. furthermore, when expressed in a cdc28ts saccharomyces strain, ... | 1991 | 1840925 |
| amino acid sequence of the alpha and beta subunits of methanosarcina barkeri atpase deduced from cloned genes. similarity to subunits of eukaryotic vacuolar and f0f1-atpases. | the atpa and atpb genes coding for the alpha and beta subunits, respectively, of membrane atpase were cloned from a methanogen methanosarcina barkeri, and the amino acid sequences of the two subunits were deduced from the nucleotide sequences. the methanogenic alpha (578 amino acid residues) and beta (459 amino acid residues) subunits were highly homologous to the large and small subunits, respectively, of vacuolar h+-atpases; 52% of the residues of the methanogenic alpha subunit were identical ... | 1989 | 2544575 |
| high-frequency t-dna-mediated gene tagging in plants. | an insertion element [transferred dna (t-dna)], transferred by soil agrobacteria into the nuclear genome of plants, was used for induction of gene fusions in arabidopsis thaliana, nicotiana tabacum, and nicotiana plumbaginifolia. a promoterless aph(3')ii (aminoglycoside phosphotransferase ii) reporter gene was linked to the right end of the t-dna and transformed into plants along with a plasmid replicon and a selectable hygromycin-resistance gene. transcriptional and translational reporter gene ... | 1989 | 2554318 |
| the ubiquitin-encoding multigene family of flax, linum usitatissimum. | ubiquitin (ubq), a 76-amino acid (aa) protein, is found in all eukaryotic organisms and is one of the most conserved proteins so far studied. it is implicated in many cellular processes. the ubq-encoding genes (ubq) are generally present as a multigene family. in flax, we have estimated that this multigene family contains at the most ten members. the initial flax ubq sequences were isolated from a flax genomic library in lambda embl4 using a heterologous arabidopsis thaliana ubq probe. an 916-bp ... | 1991 | 1850710 |
| 3-hydroxy-3-methylglutaryl-coenzyme a reductase from arabidopsis thaliana is structurally distinct from the yeast and animal enzymes. | we have isolated the arabidopsis thaliana gene (hmg1) encoding 3-hydroxy-3-methylglutaryl-coa reductase [hmg-coa reductase; (s)-mevalonate:nad+ oxido-reductase (coa-acylating), ec 1.1.1.88], the catalyst of the first committed step in isoprenoid biosynthesis. cdna copies of the plant gene were identified by hybridization with a short, highly conserved segment of yeast hmg-coa reductase as probe. dna sequence analysis reveals that the cooh-terminal domain of the arabidopsis hmg-coa reductase (con ... | 1989 | 2649893 |
| cloning and chromosomal mapping of nuclear genes encoding chloroplast and cytosolic glyceraldehyde-3-phosphate-dehydrogenase from arabidopsis thaliana. | both cdna and genomic clones for the nuclear genes encoding chloroplast (cp) (gapa and gapb) and cytosolic (gapc) glyceraldehyde-3-phosphate dehydrogenase (gapdh) from arabidopsis thaliana have been isolated and characterized. genomic southern-blot analyses indicate that there is only one copy of each gapa, gapb and gapc gene in a. thaliana. comparison of the deduced amino acid (aa) sequences shows that the a and b subunits are highly similar (80% positional aa identity), while there is less sim ... | 1991 | 1916285 |
| the major low-molecular-weight heat shock protein in chloroplasts shows antigenic conservation among diverse higher plant species. | several plant species are known to synthesize low-molecular-weight nucleus-encoded heat shock proteins (hsps) which localize to chloroplasts. dna sequence analysis of chloroplast hsp cdnas from pea (pisum sativum) and soybean (glycine max) has shown that the carboxyl-terminal halves of these proteins are homologous to low-molecular-weight hsps from a wide range of eucaryotes (e. vierling, r. t. nagao, a. e. derocher, and l. m. harris, embo j. 7:575-581, 1988). we used a pea cdna to construct fus ... | 1989 | 2710111 |
| a cacgtg motif of the antirrhinum majus chalcone synthase promoter is recognized by an evolutionarily conserved nuclear protein. | in the chalcone synthase gene of antirrhinum majus (snapdragon), 150 base pairs of the 5' flanking region contain cis-acting signals for uv light-induced expression. a nuclear factor, designated cg-1, specifically recognizes a hexameric motif with internal dyad symmetry, cacgtg, located within this light-responsive sequence. binding of cg-1 is influenced by c-methylation of the cpg dinucleotide in the recognition sequence. cg-1 is a factor found in a variety of dicotyledonous plant species inclu ... | 1989 | 2780550 |
| a steroid-inducible gene expression system for plant cells. | promoters that allow the selective induction of gene expression in vivo constitute an important methodology in eukaryotic organisms such as yeast and the fruit fly, but to date no such system has been described for higher plants. given the fact that mammalian steroid receptors can function as hormone-dependent inducers of gene expression in heterologous systems, the feasibility of using mammalian steroid hormones as selective inducers of plant gene expression was investigated. here it is shown t ... | 1991 | 1961707 |
| identification of an essential tyrosine residue in the catalytic site of a chitinase isolated from zea mays that is selectively modified during inactivation with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide. | chitinase isolated from zea mays seeds is inactivated by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (edc) in the absence of exogenous nucleophiles. oligomers of n-acetylglucosamine,n,n',n",n"'-tetra-n-acetylchitotetraose (glcnac4), and to a lesser extent, n,n',n"-tri-n-acetylchitotriose (glcnac3) and n,n'-di-n-acetylchitobiose (glcnac2) provide partial protection against inactivation by the reagent. an examination of the concentration dependence of the protection afforded by glcnac4 revealed ... | 1992 | 1740436 |
| identification of upstream regulatory elements involved in the developmental expression of the arabidopsis thaliana cab1 gene. | we studied cis regulatory elements controlling the light-dependent organ-specific expression of arabidopsis thaliana chlorophyll a/b binding protein gene (cab1) by stably transforming tobacco plants using a tumor-inducing (ti) plasmid vector system. the results from the 5' and internal deletion analyses indicate that there are at least three cis-acting elements that are involved in the light-dependent developmental expression of cab1 gene. two such elements are located at the immediate upstream ... | 1988 | 3054877 |
| intron existence predated the divergence of eukaryotes and prokaryotes. | nucleotide sequences for the nuclear genes encoding chloroplast (gapa and gapb) and cytosolic (gapc) glyceraldehyde-3-phosphate dehydrogenases (gapdhs) from arabidopsis thaliana were determined. comparison of nucleotide sequences indicates that the divergence of chloroplast and cytosolic gapdh genes preceded the divergence of prokaryotes and eukaryotes. in addition, some intron-exon junctions are conserved among gapb, gapc, and chicken gapdh genes. these results provide evidence at the molecular ... | 1988 | 3055302 |
| specialized binary vector for plant transformation: expression of the arabidopsis thaliana ahas gene in nicotiana tabacum. | we constructed a cosmid vector, poca18, designed for transferring plant genomic libraries from agrobacterium tumefaciens to plants. clones from a genomic library of arabidopsis thaliana dna in poca 18 were propagated stably in both escherichia coli and a. tumefaciens. clones from the poca18 a. thaliana library were used to construct transgenic nicotiana tabacum plants; the dna inserts were transferred intact in 10 out of 16 transgenic n. tabacum plants examined but were partially deleted in six ... | 1988 | 3060849 |
| characterization of two genes encoding small heat-shock proteins in arabidopsis thaliana. | using the technique of differential hybridization screening, we have isolated the cdnas for two low-molecular-mass heat-shock proteins and their corresponding genes, hsp17.4 and hsp18.2, from arabidopsis thaliana. these two genes encode polypeptides that are 79.2% identical to each other with respect to amino acid sequence, and contain several overlapping sequences that are similar to the consensus sequences for the heat-shock elements (hse) in drosophila in the regions upstream from the promote ... | 1989 | 2482931 |
| structure and expression of spinach leaf cdna encoding ribulosebisphosphate carboxylase/oxygenase activase. | ribulosebisphosphate carboxylase/oxygenase activase is a recently discovered enzyme that catalyzes the activation of ribulose-1,5-bisphosphate carboxylase/oxygenase ["rubisco"; ribulose-bisphosphate carboxylase; 3-phospho-d-glycerate carboxy-lyase (dimerizing), ec 4.1.1.39] in vivo. clones of rubisco activase cdna were isolated immunologically from spinach (spinacea oleracea l.) and arabidopsis thaliana libraries. sequence analysis of the spinach and arabidopsis cdnas identified consensus nucleo ... | 1988 | 3277181 |
| isolation, expression and phylogenetic inheritance of an acetolactate synthase gene from brassica napus. | an acetolactate synthase gene was isolated and characterized from brassica napus. this b. napus acetolactate synthase gene encodes a deduced polypeptide sequence of 637 amino acids which is 85% homologous to the corresponding proposed gene product from arabidopsis thaliana. peptide domains recently associated with herbicide resistance/sensitivity are conserved between the two sequences. from southern analysis we conclude that the gene isolated is one member of a multigene acetolactate synthase g ... | 1989 | 2482934 |
| transcriptional regulation of the arabidopsis thaliana chalcone synthase gene. | we have cloned an arabidopsis thaliana chalcone synthase (chs) gene on the basis of cross-hybridization with a petroselinum hortense chs cdna clone. the protein sequence deduced from the a. thaliana chs dna sequence is at least 85% homologous to the chs sequences from p. hortense, antirrhinum majus, and petunia hybrida. southern blot analysis indicated that chs is a single-copy gene in a. thaliana. high-intensity light treatment of a. thaliana plants for 24 h caused a 50-fold increase in chs enz ... | 1988 | 3386631 |
| use of the dna polymerase chain reaction for homology probing: isolation of partial cdna or genomic clones encoding the iron-sulfur protein of succinate dehydrogenase from several species. | the dna polymerase chain reaction was developed for in vitro amplification of specific dna sequences, and it has been used for a wide variety of purposes in several fields. we have developed an application of the polymerase chain reaction that is useful for the isolation of partial cdna or genomic clones of conserved genes. we used this technique to clone the gene encoding the iron protein subunit (27 kda) of succinate dehydrogenase (ec 1.3.5.1) from several species, including human, rat, drosop ... | 1989 | 2494655 |
| structure of a plasma membrane h+-atpase gene from the plant arabidopsis thaliana. | physiological and biochemical studies have suggested that the plant plasma membrane h+-atpase controls many important aspects of plant physiology, including growth, development, nutrient transport, and stomata movements. we have started the genetic analysis of this enzyme by isolating both genomic and cdna clones of an h+-atpase gene from arabidopsis thaliana. the cloned gene is interrupted by 15 introns, and there is partial conservation of exon boundaries with respect to animal (na+/k+)- and c ... | 1989 | 2524481 |
| a binary vector for transferring genomic libraries to plants. | the transformation of mutant plants with a complete recombinant library derived from wild-type dna followed by assay of transformed plants for complementation of the mutant phenotype is a promising method for the isolation of plant genes. the small genome of arabidopsis thaliana is a good candidate for attempting this so-called shotgun transformation. we present the properties of an a. thaliana genomic library cloned in a binary vector, pc22. this vector, designed to introduce genomic libraries ... | 1986 | 3534794 |
| [study of descendants of "arabidopsis thaliana" plants treated with bacterial dna. i. presence and replication of exogenous dna]. | | 1970 | 4098023 |
| [fate of exogenous dna during the growth and development of "arabidopsis thaliana"]. | | 1969 | 4181702 |
| the plant g box promoter sequence activates transcription in saccharomyces cerevisiae and is bound in vitro by a yeast activity similar to gbf, the plant g box binding factor. | g box and i box sequences of the arabidopsis thaliana ribulose-bisphosphate-1,5-carboxylase small subunit (rbcs) promoter are required for expression mediated by the arabidopsis rbcs-1a promoter in transgenic tobacco plants and are bound in vitro by factors from plant nuclear extracts termed gbf and ga-1, respectively. we show here that a -390 to -60 rbcs-1a promoter fragment containing the g box and two i boxes activates transcription from a truncated iso-1-cytochrome c (cyc1) gene promoter in ... | 1990 | 2161333 |
| molecular cloning of a family of plant genes encoding a protein homologous to plasma membrane h+-translocating atpases. | cdna clones from nicotiana plumbaginifolia have been isolated by hybridization to a yeast h+-atpase gene. the largest one encodes a polypeptide (pma2) of 956 amino acid residues which exhibits a homology of 73% with a limited protein sequence obtained from purified oat plasma membrane h+-atpase (schaller and sussman, plant physiol. 86, 512-516, 1988) and an 82% homology with the arabidopsis thaliana pma gene (harper et al., proc. natl. acad. sci. usa 86, 1234-1238). it is therefore concluded tha ... | 1989 | 2527030 |
| arabidopsis thaliana tryptophan synthase alpha: gene cloning, expression, and subunit interaction. | the tryptophan synthase alpha subunit catalyzes the conversion of indole-3-glycerolphosphate to indole, the penultimate reaction in the biosynthesis of the essential amino acid tryptophan. a cdna encoding arabidopsis thaliana tryptophan synthase alpha(tsa1) was isolated by complementation of an escherichia coli delta trpa mutation and by polymerase chain reaction amplification from a cdna library using degenerate primers. a tsa1 genomic clone was also isolated and 5 kb of the dna sequence determ ... | 1995 | 7476868 |
| ubiquitin extension proteins of arabidopsis thaliana. structure, localization, and expression of their promoters in transgenic tobacco. | the highly conserved protein ubiquitin is synthesized in eukaryotes as two types of protein fusions from which active ubiquitin is derived by proteolytic processing. we report here the isolation and characterization of multiple genes from one type that encode ubiquitin extension proteins from the higher plant, arabidopsis thaliana (l.). two genes with 90% nucleotide identity in their exons encode ubiquitin and identical 52-amino acid (aa) extension proteins with 85 and 79% aa identity to 52-aa e ... | 1990 | 2165066 |
| homologous domains of the largest subunit of eucaryotic rna polymerase ii are conserved in plants. | genomic and cdna clones homologous to the rpii215 gene of drosophila were isolated from arabidopsis thaliana and assigned to a single copy gene encoding a transcript of 6.8 kb. nucleotide sequence analysis of arabidopsis genomic and cdnas revealed a striking homology to yeast, caenorhabditis, drosophila and mouse genes encoding the largest subunit of rna polymerase ii. the arabidopsis gene rpii215 contains 13 introns, 12 of which interrupt the coding sequence of a protein of 205 kda. the positio ... | 1990 | 2259344 |
| modification of the substrate specificity of an acyl-acyl carrier protein thioesterase by protein engineering. | the plant acyl-acyl carrier protein (acp) thioesterases (tes) are of biochemical interest because of their roles in fatty acid synthesis and their utilities in the bioengineering of plant seed oils. when the fatb1 cdna encoding a 12:0-acp te (uc fatb1) from california bay, umbellularia californica (uc) was expressed in escherichia coli and in developing oilseeds of the plants arabidopsis thaliana and brassica napus, large amounts of laurate (12:0) and small amounts of myristate (14:0) were accum ... | 1995 | 7479856 |
| molecular cloning and characterization of genes expressed in shoot apical meristems. | the above-ground portion of a plant develops from the shoot apical meristem. an abundant source of apical meristems was obtained from cauliflower heads. meristematic cdnas were identified by differential screening and used to isolate corresponding arabidopsis thaliana genes. transcriptional promoters from arabidopsis clones were fused to the beta-glucuronidase (gus) reporter gene and introduced into plants, and gus expression was used to analyze temporal and spatial regulation of the promoters. ... | 1991 | 1840916 |
| strong cellular preference in the expression of a housekeeping gene of arabidopsis thaliana encoding s-adenosylmethionine synthetase. | s-adenosylmethionine serves as a methyl group donor in numerous transmethylation reactions and plays a role in the biosynthesis of polyamines and ethylene. we have cloned and sequenced an s-adenosylmethionine synthetase gene (sam-1) of arabidopsis thaliana. the deduced polypeptide sequence of the enzyme has extensive homology with the corresponding enzymes of escherichia coli and yeast. genomic hybridization indicates the presence of two adenosylmethionine synthetase genes per haploid arabidopsi ... | 1989 | 2535470 |
| evidence that plant k+ channel proteins have two different types of subunits. | plant k+ channel proteins have been previously characterized as tetramers of membrane-spanning alpha subunit polypeptides. recent studies have identified a 39-kd, hydrophilic polypeptide that is a structural component of purified animal k+ channel proteins. we have cloned and sequenced an arabidopsis thaliana cdna encoding a 38.4-kd polypeptide that has a sequence homologous to the animal k+ channel beta subunit. southern and northern analyses indicate the presence of a gene encoding this cdna i ... | 1995 | 7480329 |
| molecular cloning and characterization of the cdna coding for the biotin-containing subunit of the chloroplastic acetyl-coenzyme a carboxylase. | we report the molecular cloning and sequence of the cdna coding for the biotin-containing subunit of the chloroplastic acetylcoenzyme a (coa) carboxylase (accase) of arabidopsis thaliana (cac1). the 3' end of the cac1 sequence, coding for a peptide of 94 amino acids, which includes a putative biotinylation motif, was expressed in escherichia coli as a glutathione-s-transferase (gst) fusion protein. the resulting gst-cac1 fusion protein was biotinylated in vivo, indicating that cac1 codes for a b ... | 1995 | 7480350 |
| multiple isoforms of arabidopsis casein kinase i combine conserved catalytic domains with variable carboxyl-terminal extensions. | three cdna clones encoding isoforms of casein kinase i (cki) were isolated from arabidopsis thaliana. one full-length clone, designated cki1, contained an open reading frame of 1371 bp encoding a protein of 51,949 d with an isoelectric point of 9.7. in addition to the highly conserved catalytic domain (of about 300 amino acids), the arabidopsis cki isoforms contain 150 to 180 amino acid carboxyl-terminal extensions, which show among themselves a lower level of sequence conservation. these extens ... | 1995 | 7480353 |
| adenosine-5'-triphosphate-sulfurylase from arabidopsis thaliana and escherichia coli are functionally equivalent but structurally and kinetically divergent: nucleotide sequence of two adenosine-5'-triphosphate-sulfurylase cdnas from arabidopsis thaliana and analysis of a recombinant enzyme. | atp-sulfurylase, the first enzyme of sulfate assimilation, catalyzes the formation of adenosine-5'-phosphosulfate from atp and sulfate. here we report that the higher plant, arabidopsis thaliana, contains a three-member, expressed gene family encoding plastid localized forms of atp sulfurylase. three cdnas from a. thaliana, designated aps1, aps2, and aps3, were isolated by their ability to functionally complement a met3 (atp sulfurylase) mutant strain of saccharomyces cerevisiae (yeast). the nuc ... | 1995 | 7487067 |
| photoregulation of a phytochrome gene promoter from oat transferred into rice by particle bombardment. | the regulatory photoreceptor phytochrome controls the transcription of its own phy genes in a negative feedback fashion. we have exploited microprojectile-mediated gene transfer to develop a rapid transient expression assay system for the study of dna sequences involved in the phytochrome-regulated expression of these genes. the 5'-flanking sequence and part of the structural region of an oat phy gene have been fused to a reporter coding sequence (chloramphenicol acetyltransferase, cat) and intr ... | 1989 | 2602370 |
| lambda yes: a multifunctional cdna expression vector for the isolation of genes by complementation of yeast and escherichia coli mutations. | this work describes a multifunctional phage lambda expression vector system, lambda yes, designed to facilitate gene isolation from eukaryotes by complementation of escherichia coli and saccharomyces cerevisiae mutations. lambda yes vectors have a selection for cdna inserts using an oligo adaptor strategy and are capable of expressing genes in both e. coli and s. cerevisiae. they also allow conversion from phage lambda to plasmid clones by using the cre-lox site-specific recombination system, re ... | 1991 | 1848010 |
| conserved domains in molybdenum hydroxylases. the amino acid sequence of chicken hepatic sulfite oxidase. | the amino acid sequence of the molybdenum-containing domain of chicken hepatic sulfite oxidase has been determined by edman degradation of the purified protein. combining these data with those previously published for the heme-containing domain (guiard, b., and lederer, f. (1979) eur. j. biochem. 100, 441-453) indicates that each subunit of the homodimer comprises a single polypeptide chain containing 460 amino acid residues (mr = 50,545). comparison of the sequence with the cdna-deduced sequenc ... | 1989 | 2687265 |
| characterization of cdna for a dehydration-inducible gene that encodes a clp a, b-like protein in arabidopsis thaliana l. | sequence was obtained from a cdna clone, designated erd1, isolated from a cdna library of 1-hour-dehydrated plants of arabidopsis thaliana l. the clone (3150 bp) contains an open reading frame of 946 amino acid residues with greater than 34% sequence identity to the regulatory subunit of the clp atp-dependent protease in escherichia coli and contains a putative chloroplast-targeting signal at the n-terminus. southern blot analysis suggested the presence of additional erd1-related genes in a. tha ... | 1993 | 7504470 |
| gap junction protein homologue from arabidopsis thaliana: evidence for connexins in plants. | an arabidopsis thaliana library constructed in the pbluescript expression vector lambda zap ii (stratagene) was screened with three affinity-purified antibodies raised against (i) rat liver connexin 32, (ii) a polypeptide from soybean root cells that migrates with a molecular mass of 29 kda in sds/polyacrylamide gels and is immunologically related to rat liver connexin, and (iii) a synthetic peptide corresponding to a sequence in rat liver connexin 32. a single clone was obtained whose gene prod ... | 1991 | 1851993 |
| amplification of plant u3 and u6 snrna gene sequences using primers specific for an upstream promoter element and conserved intragenic regions. | u-snrna genes in higher plants contain two essential promoter elements, the use with sequence rtcccacatcg and the tata-like box, positioned in the -70 and -30 regions, respectively. using an oligodeoxynucleotide containing the use motif and oligodeoxynucleotides specific for the intragenic regions conserved in u-snrnas, several sequences encoding u6 and u3 snrnas were determined by polymerase chain reaction (pcr) amplification of arabidopsis thaliana and tobacco genomic dnas. this method provide ... | 1990 | 2362803 |
| illegitimate recombination in plants: a model for t-dna integration. | agrobacterium tumefaciens is a soil bacterium capable of transferring dna (the t-dna) to the genome of higher plants, where it is then stably integrated. six t-dna inserts and their corresponding preinsertion sites were cloned from arabidopsis thaliana and analyzed. two t-dna integration events from nicotiana tabacum were included in the analysis. nucleotide sequence comparison of plant target sites before and after t-dna integration showed that the t-dna usually causes only a small (13-28 bp) d ... | 1991 | 1995418 |
| the arabidopsis thaliana apurinic endonuclease arp reduces human transcription factors fos and jun. | an arabidopsis thaliana cdna encoding an analogue, referred to as arp for apurinic endonuclease-redox protein, of the human redox factor ref has been cloned. arp stimulates in vitro dna-binding activity of the human transcription factor jun and fos by the reduction of a cysteine residue located in the dna-binding domain. based on amino acid sequence homology, this redox activity is probably confined to the small internal domain of the arp protein. in analogy to ref, we show that the arabidopsis ... | 1994 | 7512729 |
| the mutagenicity of azido derivatives of monosaccharides and alcohols and of n-(3-azido-2-hydroxypropyl) derivatives of purines and pyrimidines in arabidopsis thaliana and in salmonella typhimurium. | the azido derivatives of alcohols (3-azido-1,2-propandiol and 1,3-diazido-2-propanol) and monosaccharides (6-azido-6-deoxy-beta-d-glucose and 6-azido-6-deoxy-beta-d-galactose), as well as the proximal mutagenic product of sodium azide metabolism beta-azido-l-alanine, exhibited a high mutagenic activity in a higher plant arabidopsis thaliana and in salmonella typhimurium. in contrast, 11 n-(3-azido-2-hydroxypropyl) derivatives of purines and pyrimidines (adenine, thymine, uracil, cytosine, 2-amin ... | 1991 | 2038273 |
| characterization of some major identity elements in plant alanine and phenylalanine transfer rnas. | alanine and phenylalanine trna sequences were amplified by pcr from arabidopsis thaliana nuclear dna using degenerate oligonucleotides which introduced specific mutations into the acceptor stem. the aminoacylation of t7 rna polymerase transcripts of these sequences was investigated in vitro using partially purified bean alanyl- or phenylalanyl-trna synthetase. in parallel, the in vivo activity of amber suppressor derivatives of these trnas was investigated in transient expression assays in tobac ... | 1994 | 7532029 |
| tissue-specific expression directed by an arabidopsis thaliana pre-ferredoxin promoter in transgenic tobacco plants. | we have isolated and analyzed a pre-ferredoxin gene from arabidopsis thaliana. this gene encodes a 148 amino acid precursor protein including a chloroplast transit peptide of 52 residues. southern analysis shows the presence of a single copy of this ferredoxin (fd) gene in the a. thaliana genome. its expression is tissue-specific and positively affected by light. response times, both to dark and light conditions, are remarkably rapid. a chimeric gene consisting of a 1.2 kb fd promoter fragment f ... | 1990 | 2102830 |
| isolation of single-copy-sequence clones from a yeast artificial chromosome library of randomly-sheared arabidopsis thaliana dna. | we describe the construction of a yeast artificial chromosome (yac) library from the arabidopsis thaliana genome. randomly sheared high molecular weight source dna was extracted from frozen, ground leaf tissue and blunt-end-ligated to the vector pyac3. by size-fractionating the ligation products, we achieved an average clone size of 150 kb. approximately 6% of the yacs contained inserts from the chloroplast genome. we screened clones equivalent to greater than four a. thaliana haploid nuclear ge ... | 1990 | 2102834 |
| isolation of a cdna encoding a novel gtp-binding protein of arabidopsis thaliana. | a cdna encoding for a 68 kda gtp-binding protein was isolated from arabidopsis thaliana (ag68). this clone is a member of a gene family that codes for a class of large gtp-binding proteins. this includes the mammalian dynamin, yeast vps1p and the vertebrate mx proteins. the predicted amino acid sequence was found to have high sequence conservation in the n-terminal gtp-binding domain sharing 54% identity to yeast vps1p, 56% amino acid identity to rat dynamin and 38% identity to the murine mx1 pr ... | 1995 | 7548829 |
| the arabidopsis thaliana plasma membrane h(+)-atpase multigene family. genomic sequence and expression of a third isoform. | the plasma membrane of higher plants contains a h(+)-atpase as its major ion pump. this enzyme belongs to the p-type family of cation-translocating enzymes and generates the proton-motive force that drives solute uptake across the plasma membrane. in arabidopsis thaliana the plasma membrane h(+)-atpase is encoded by a multigene family (harper, j. f., surowy, t. k., and sussman, m. r. (1989) proc. natl. acad. sci. u. s. a. 86, 1234-1238). the complete genomic sequence of a third arabidopsis h(+)- ... | 1990 | 2143186 |
| efficient isolation and mapping of arabidopsis thaliana t-dna insert junctions by thermal asymmetric interlaced pcr. | thermal asymmetric interlaced (tail-) pcr is an efficient technique for amplifying insert ends from yeast artificial chromosome (yac) and p1 clones. highly specific amplification is achieved without resort to complex manipulations before or after pcr. the adaptation of this method for recovery and mapping of genomic sequences flanking t-dna insertions in arabidopsis thaliana is described. insertion-specific products were amplified from 183 of 190 tested t-dna insertion lines. reconstruction expe ... | 1995 | 7550382 |
| isolation of a gene encoding a novel chloroplast protein by t-dna tagging in arabidopsis thaliana. | a recessive pale mutation, designated as cs, was identified by transferred-dna (t-dna)-mediated insertional mutagenesis in arabidopsis thaliana. the pale mutation, cosegregating with the hygromycin resistance marker of the t-dna, was mapped to the position of the ch-42 (chlorata) locus on chromosome 4. lack of genetic complementation between cs and ch-42 mutants indicated allelism. plant boundaries of the t-dna insert rescued from the pale mutant were used as probes for the isolation of genomic ... | 1990 | 2158442 |
| isolation and characterization of the nitrate reductase structural gene of chlamydomonas reinhardtii. | the nitrate reductase structural gene of chlamydomonas reinhardtii has been isolated from a genomic library by using a nitrate reductase cdna probe from barley. restriction fragment length polymorphism analyses mapped the chlamydomonas clone (b6a) to the nitrate reductase structural gene locus nit-1. overlapping inserts cover a region of the genome of about 24 kilobases containing the entire gene, which spans approximately 5-8 kilobases. sequence analysis of dna fragments from the b6a clone demo ... | 1989 | 2475871 |
| structure and expression of the u5 snrna gene of arabidopsis thaliana. conserved upstream sequence elements in plant u-rna genes. | we have previously characterized the u2 small nuclear (sn) rna gene family of arabidopsis thaliana. to find out the structural features of upstream and downstream non-coding regions that are shared by different u-rna genes in higher plants we have isolated the gene encoding a 125 nt-long u5 snrna of arabidopsis. activity of the cloned gene was demonstrated in stably transformed tobacco calli and by transient expression in transfected protoplasts of nicotiana plumbaginifolia. southern analysis in ... | 1988 | 3205713 |
| isolation of a higher eukaryotic telomere from arabidopsis thaliana. | we have developed a method for constructing genomic libraries enriched for telomeric dna sequences, enabling the isolation of telomeres from higher eukaryotic organisms with large chromosomes. the method was used to clone telomeric dna sequences from the flowering plant arabidopsis thaliana. a. thaliana telomeres are composed primarily of tandemly repeated blocks of the sequence 5'-ccctaaa-3' and are heterogeneous in size. genomic sequences that cross-hybridize at high stringency with a. thalian ... | 1988 | 3349525 |
| isolation of a novel arabidopsis ozone-induced cdna by differential display. | we have used a reverse transcriptase polymerase chain reaction procedure (differential display) to isolate cdnas corresponding to transcripts that accumulate in ozone-treated arabidopsis thaliana. in this report we describe the characterization of an ozone-induced transcript, atozi1. atozi1 mrna in untreated plants was detected at low levels in cotyledons, leaves, and flower buds and at higher levels in roots and mature flowers. atozi1 mrna accumulation was transiently induced in leaves 3- to 5- ... | 1995 | 7579170 |
| evidence for proteolytic processing of tobacco mosaic virus movement protein in arabidopsis thaliana. | two ecotypes of arabidopsis thaliana were transformed with the gene encoding tobacco mosaic virus (tmv) movement protein (p30). p30 accumulated largely in a subcellular fraction containing cell wall components and as a soluble protein. the protein migrated in denaturing gels with an m(r) of 30k, significantly faster than p30 (m(r) approximately 34k) accumulating after expression in transgenic tobacco, escherichia coli or spodoptera frugiperda cells, or after virus multiplication in tobacco. the ... | 1995 | 7579611 |
| mitochondrial citrate synthase from potato: predominant expression in mature leaves and young flower buds. | a cdna clone encoding mitochondrial citrate synthase (ec 4.1.3.7), the first enzyme of the tricarboxylic-acid cycle, was isolated from potato (solanum tuberosum l.) and expression of the enzyme analyzed. the deduced amino-acid sequence of the potato mitochondrial citrate synthase showed high similarity to known citrate synthases from fungi, mammals and arabidopsis thaliana. the expression pattern of this clone was determined by northern blot analysis. expression was detected in all tissues analy ... | 1995 | 7580855 |
| high-frequency and efficient agrobacterium-mediated transformation of arabidopsis thaliana ecotypes "c24" and "landsberg erecta" using agrobacterium tumefaciens. | | 1995 | 7581660 |
| cloning and sequencing of a 36-kb region of the bacillus subtilis genome between the gnt and iol operons. | within the framework of an international project for the sequencing of the entire bacillus subtilis genome, a 36-kb chromosome segment, which covers the region between the gnt and iol operons, has been cloned and sequenced. this region (36447 bp) contains 33 complete open reading frames (orfs; genes) including the four gnt genes and one partial gene. a homology search for the products of the 33 complete orfs revealed significant homology to known proteins in 16 of them such as tetracycline resis ... | 1995 | 7584049 |
| structural analysis of a reca-like gene in the genome of arabidopsis thaliana. | a reca-like gene was identified in the genome of arabidopsis thaliana by means of pcr using primers designed on the basis of previously reported amino acid sequences of eukaryotic reca-like proteins. the structure of the gene, termed arlim15, was investigated by comparing the primary structure of the genomic dna with that of the corresponding cdna. the open reading frame, which was split into 15 exons, was established to have the capacity for encoding a 37.3-kda polypeptide. the amino acid seque ... | 1995 | 7584052 |
| 2,3-bisphosphoglycerate-independent phosphoglycerate mutase is conserved among different phylogenic kingdoms. | we have previously demonstrated that maize (zea mays) 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (pgam-i) is not related to 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase. with the aid of specific anti-maize pgam-i antibodies, we demonstrate here the presence of a closely related pgam-i in other plants. we also describe the isolation and sequencing of a cdna-encoding almond (prunus amygdalus) pgam-i that further demonstrates this relationship among plant pgam-i. a sea ... | 1995 | 7584858 |
| bacterial expression of the catalytic domain of 3-hydroxy-3-methylglutaryl-coa reductase (isoform hmgr1) from arabidopsis thaliana, and its inactivation by phosphorylation at ser577 by brassica oleracea 3-hydroxy-3-methylglutaryl-coa reductase kinase. | the catalytic domain of 3-hydroxy-3-methylglutaryl-coa reductase isoform 1 (hmgr1cd) from arabidopsis thaliana has been expressed in escherichia coli in a catalytically active form and purified. the high efficiency of the bacterial expression system together with the simplicity of the purification procedure used in this study resulted in the attainment of large quantities of pure enzyme (about 5 mg/l culture) with a final specific activity of up to 17 u/mg. this specific activity is higher than ... | 1995 | 7588795 |
| molecular characterization of an arabidopsis thaliana cdna encoding a novel putative adenylate translocator of higher plants. | we have isolated an arabidopsis thaliana cdna encoding a highly hydrophobic membrane protein of 589 amino acids which contains 12 potential transmembrane helices and shows a high degree of similarity (43.5% identity, 66.2% similarity) to the atp/adp translocase of the gram-negative bacterium rickettsia prowazekii, an obligate intracellular parasite responsible for the epidemic typhus. this rickettsial translocator resides in the cytoplasmic membrane and allows the bacterium to exploit the host c ... | 1995 | 7589569 |
| sequence and nitrate regulation of the arabidopsis thaliana mrna encoding nitrate reductase, a metalloflavoprotein with three functional domains. | the sequence of nitrate reductase (ec 1.6.6.1) mrna from the plant arabidopsis thaliana has been determined. a 3.0-kilobase-long cdna was isolated from a lambda gt10 cdna library of arabidopsis leaf poly(a)+ rna. the cdna hybridized to a 3.2-kilobase mrna whose level increased 15-fold in response to treatment of the plant with nitrate. an open reading frame encoding a 917 amino acid protein was found in the sequence. this protein is very similar to tobacco nitrate reductase, being greater than 8 ... | 1988 | 3393528 |
| identification of strong modifications in cation selectivity in an arabidopsis inward rectifying potassium channel by mutant selection in yeast. | the arabidopsis thaliana cdna, kat1, encodes a hyperpolarization-activated k+ channel. in the present study, we utilized a combination of random site-directed mutagenesis, genetic screening in a potassium uptake-deficient yeast strain, and electrophysiological analysis in xenopus oocytes to identify strong modifications in cation selectivity of the inward rectifying k+ channel kat1. threonine at position 256 was replaced by 11 other amino acid residues. six of these mutated kat1 cdnas complement ... | 1995 | 7592636 |
| arabidopsis thaliana nadph oxidoreductase homologs confer tolerance of yeasts toward the thiol-oxidizing drug diamide. | to isolate new plant genes involved in the defense against oxidative stress, an arabidopsis cdna library in a yeast expression vector was transformed into a yeast strain deficient in the yap1 gene, which encodes a b-zip transcription factor and regulates general stress response in yeasts. cells from approximately 10(5) primary transformants were subjected to a tolerance screen toward the thiol-oxidizing drug diamide, which depletes the reduced glutathione in the cell. four types of arabidopsis c ... | 1995 | 7592828 |
| characterisation of the telomeres at opposite ends of a 3 mb theileria parva chromosome. | bacteriophage lambda clones containing theileria parva genomic dna derived from two different telomeres were isolated and the nucleotide sequences of the telomeric repeats and adjacent telomere-associated (tas) dna were determined. the t.parva telomeric repeat sequences, a tandem array of ttttaggg or tttaggg interspersed with a few variant copies, showed a high degree of sequence identity to those of the photosynthetic algae chlamydomonas reinhardtii (97% identity) and chlorella vulgaris (87.7% ... | 1995 | 7596822 |
| characterization of the alpha-tubulin gene family of arabidopsis thaliana. | the genome of arabidopsis thaliana (linnaeus) heynhold was shown to contain an alpha-tubulin gene family consisting of at least four genes and/or pseudogenes. the primary structure of a transcribed alpha-tubulin gene was determined. a comparison of the predicted amino acid sequence of the a. thaliana alpha-tubulin with the predicted amino acid sequences of alpha-tubulins of chlamydomonas reinhardtii, stylonychia lemnae, and homo spaiens reveals a high degree of homology; 90%, 87%, and 83% identi ... | 1987 | 3475704 |
| [redistribution, at the time of blossoming, of exogenous dna absorbed by arabidopsis thaliana seeds]. | | 1969 | 4186392 |
| fate of exogenous dna in arabidopsis thaliana. translocation and integration. | | 1971 | 4942550 |
| a two-element enhancer-inhibitor transposon system in arabidopsis thaliana. | the enhancer-inhibitor (en-i), also known as suppressor-mutator (spm-dspm), transposable element system of maize was modified and introduced into arabidopsis by agrobacterium tumefaciens transformation. a stable en/spm transposase source under control of the camv 35s promoter mediated frequent transposition of i/dspm elements. transposition occurred continuously throughout plant development over at least seven consecutive plant generations after transformation. new insertions were found at both ... | 1995 | 7603434 |
| organization of telomeric and subtelomeric chromatin in the higher plant nicotiana tabacum. | we have examined the structure and chromatin organization of telomeres in nicotiana tabacum. in tobacco the blocks of simple telomeric repeats (tt-taggg)n are many times larger than in other plants, e.g., arabidopsis thaliana or tomato. they are resolved as multiple fragments 60-160 kb in size (in most cases 90-130 kb) on pulsed-field gel electrophoresis (pfge) of restriction endonuclease-digested dna. the major subtelomeric repeat of the hrs60 family forms large homogeneous blocks of a basic 18 ... | 1995 | 7603443 |
| the ga5 locus of arabidopsis thaliana encodes a multifunctional gibberellin 20-oxidase: molecular cloning and functional expression. | the biosynthesis of gibberellins (gas) after ga12-aldehyde involves a series of oxidative steps that lead to the formation of bioactive gas. previously, a cdna clone encoding a ga 20-oxidase [gibberellin, 2-oxoglutarate:oxygen oxidoreductase (20-hydroxylating, oxidizing), ec 1.14.11.-] was isolated by immunoscreening a cdna library from liquid endosperm of pumpkin (cucurbita maxima l.) with antibodies against partially purified ga 20-oxidase. here, we report isolation of a genomic clone for ga 2 ... | 1995 | 7604047 |
| common virulence factors for bacterial pathogenicity in plants and animals. | a pseudomonas aeruginosa strain (ucbpp-pa14) is infectious both in an arabidopsis thaliana leaf infiltration model and in a mouse full-thickness skin burn model. ucbpp-pa14 exhibits ecotype specificity for arabidopsis, causing a range of symptoms from none to severe in four different ecotypes. in the mouse model, ucbpp-pa14 is as lethal as other well-studied p. aeruginosa strains. mutations in the ucbpp-pa14 toxa, plcs, and gaca genes resulted in a significant reduction in pathogenicity in both ... | 1995 | 7604262 |
| the tef1 box, a ubiquitous cis-acting element involved in the activation of plant genes that are highly expressed in cycling cells. | in arabidopsis thaliana, the tef1 box is a cis-acting promoter element of the ef-1 alpha a1 gene involved in the activation of transcription in meristematic tissues. the initiation of root calli in transgenic arabidopsis by 2,4-d shows that the tef1-dependent expression of the gus reporter gene is not restricted to meristematic regions but involves all of the cycling cells. hybridization experiments conducted using arabidopsis cdna clones organized in a dense array on filters, and cdna probes pr ... | 1995 | 7476873 |
| characterization of arabidopsis thaliana cdnas that render yeasts tolerant toward the thiol-oxidizing drug diamide. | diamide oxidizes cellular thiols and induces oxidative stress. to isolate plant genes which may, when overexpressed, increase tolerance of plants toward oxidative damage, an in vivo diamide tolerance screening in yeasts was used. an arabidopsis cdna library in a yeast expression vector was used to transform a yeast strain with intact antioxidant defense. cells from approximately 10(5) primary transformants were selected for resistance to diamide. three arabidopsis cdnas which confer diamide tole ... | 1995 | 7479844 |
| visualization of site-specific recombination catalyzed by a recombinase from zygosaccharomyces rouxii in arabidopsis thaliana. | excision of a dna segment can occur in arabidopsis thaliana by reciprocal recombination between two specific recombination sites (rss) when the recombinase gene (r) from zygosaccharomyces rouxii is expressed in the plant. to monitor recombination events, we generated several lines of transgenic arabidopsis plants that carried a cryptic beta-glucuronidase (gus) reporter gene which was designed in such a way that expression of the reporter gene could be induced by r gene-mediated recombination. we ... | 1995 | 7616956 |
| a secy homolog in arabidopsis thaliana. sequence of a full-length cdna clone and import of the precursor protein into chloroplasts. | proteins are translocated across the thylakoid membrane by two distinct pathways in higher plant chloroplasts, one of which is related to prokaryotic sec-dependent translocation mechanisms. secy is an essential, hydrophobic component of the membrane-bound translocase complex in bacteria, and we report here the nucleotide sequence of a full-length cdna encoding a homolog of secy from arabidopsis thaliana. the predicted protein of 551 residues includes an amino-terminal extension of approximately ... | 1995 | 7629062 |
| isolation and expression of three gibberellin 20-oxidase cdna clones from arabidopsis. | using degenerate oligonucleotide primers based on a pumpkin (cucurbita maxima) gibberellin (ga) 20-oxidase sequence, six different fragments of dioxygenase genes were amplified by polymerase chain reaction from arabidopsis thaliana genomic dna. one of these was used to isolate two different full-length cdna clones, at2301 and at2353, from shoots of the ga-deficient arabidopsis mutant ga1-2. a third, related clone, yap169, was identified in the database of expressed sequence tags. the cdna clones ... | 1995 | 7630935 |
| a conditional negative selection for arabidopsis expressing a bacterial cytosine deaminase gene. | the enzyme activity for cytosine deaminase, which converts cytosine to uracil in bacterial, is usually undetected in higher plants and animals. the enzyme also catalyzes conversion of non-toxic 5-fluorocytosine (5-fc) to 5- fluorouracil (5-fu), a toxic compound for plant growth. the gene encoding cytosine deaminase (coda) from escherichia coli was fused to cauliflower mosaic virus (camv) 35s promoter (p35s), and cloned into a binary vector plabr101. the resulting plasmid plabr102 contained two m ... | 1995 | 7632443 |
| the genome of arabidopsis thaliana. | arabidopsis thaliana is a small flowering plant that is a member of the family cruciferae. it has many characteristics--diploid genetics, rapid growth cycle, relatively low repetitive dna content, and small genome size--that recommend it as the model for a plant genome project. the current status of the genetic and physical maps, as well as efforts to sequence the genome, are presented. examples are given of genes isolated by using map-based cloning. the importance of the arabidopsis project for ... | 1995 | 7479893 |
| light-dependent and tissue-specific expression of the h-protein of the glycine decarboxylase complex. | glycine decarboxylase is a mitochondrial enzyme complex, which is the site of photorespiratory co2 and nh3 release. although the proteins that constitute the complex are located within the mitochondria, because of their intimate association with photosynthesis their expression is controlled by light. comparisons of the kinetics of mrna accumulation between the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase and the h-protein of glycine decarboxylase during the greening of etiola ... | 1995 | 7480320 |
| [the genetic consequences of the radioactive contamination of populations of arabidopsis thaliana growing in a 30-kilometer area of the accident at the chernobyl atomic electric power station]. | annually during six years since 1986 dynamics of the mutation load in arabidopsis populations was studied. frequency of the embryonic lethal mutations was studied with muller embryonic test. first two years after the accident an increase of mutation load in arabidopsis populations was observed at all levels of radioactive contamination. in the areas with contamination up to 10 mr/h the mutation load decreased to the control level by 1990. in the areas with contamination up to 130 mr/h the mutati ... | 1995 | 7489102 |
| structure of the arabidopsis rpm1 gene enabling dual specificity disease resistance. | plants can recognize pathogens through the action of disease resistance (r) genes, which confer resistance to pathogens expressing unique corresponding avirulence (avr) genes. the molecular basis of this gene-for-gene specificity is unknown. the arabidopsis thaliana rpm1 gene enables dual specificity to pathogens expressing either of two unrelated pseudomonas syringae avr genes. despite this function, rpm1 encodes a protein sharing molecular features with recently described single-specificity r ... | 1995 | 7638602 |
| molecular characterization of a putative arabidopsis thaliana copper transporter and its yeast homologue. | at the molecular level, little is known about the transport of copper across plant membranes. we have isolated an arabidopsis thaliana cdna by complementation of a mutant (ctr1-3) of saccharomyces cerevisiae defective in high affinity copper uptake. this cdna codes for a highly hydrophobic protein (copt1) of 169 amino acid residues and with three putative transmembrane domains. most noteworthy, the first 44 residues display significant homology to the methionine- and histidine-rich copper bindin ... | 1995 | 7499355 |
| conserved initiator proteins in eukaryotes. | the origin recognition complex (orc), a multisubunit protein identified in saccharomyces cerevisiae, binds to chromosomal replicators and is required for the initiation of cellular dna replication. complementary dnas (cdnas) encoding proteins related to the two largest subunits of orc were cloned from various eukaryotes. the cdnas encoding proteins related to s. cerevisiae orc1p were cloned from the budding yeast kluyveromyces lactis, the fission yeast schizosaccharomyces pombe, and human cells. ... | 1995 | 7502077 |
| isolation of cdnas encoding gtp cyclohydrolase ii from arabidopsis thaliana. | a gtp cyclohydrolase ii-encoding gene from arabidopsis thaliana was isolated through functional complementation of a mutant of escherichia coli, bsv18, deficient in this protein. the derived amino-acid sequence constitutes a polypeptide of 27 kda and shows 37-58% identity with previously published sequences of escherichia coli, bacillus subtilis, photobacterium leiognathi and p. phosphoreum. | 1995 | 7642114 |
| isolation of an arabidopsis thaliana gene encoding cycloartenol synthase by functional expression in a yeast mutant lacking lanosterol synthase by the use of a chromatographic screen. | whereas vertebrates and fungi synthesize sterols from epoxysqualene through the intermediate lanosterol, plants cyclize epoxysqualene to cycloartenol as the initial sterol. we report the cloning and characterization of cas1, an arabidopsis thaliana gene encoding cycloartenol synthase [(s)-2,3-epoxysqualene mutase (cyclizing, cycloartenol forming), ec 5.4.99.8]. a yeast mutant lacking lanosterol synthase [(s)-2,3-epoxysqualene mutase (cyclizing, lanosterol forming), ec 5.4.99.7] was transformed w ... | 1993 | 7505443 |
| an arabidopsis thaliana lipoxygenase gene can be induced by pathogens, abscisic acid, and methyl jasmonate. | we isolated and characterized a 2.8-kb, full-length, arabidopsis thaliana cdna clone encoding a lipoxygenase. dna sequence analysis showed that the deduced amino acid sequence of the arabidopsis protein is 72 to 78% similar to that of legume seed lipoxygenases. dna blot analysis indicated that arabidopsis contains a single gene, lox1, with appreciable homology to the cdna clone. rna blot analysis showed that the lox1 gene is expressed in arabidopsis leaves, roots, inflorescences, and young seedl ... | 1993 | 7506426 |
| studies on the introduction and mobility of the maize activator element in arabidopsis thaliana and daucus carota. | we have co-transformed carrot (daucus carota) and arabidopsis thaliana with an agrobacterium tumefaciens non-tumorigenic t-dna carrying the maize transposable element activator (ac) and an agrobacterium rhizogenes ri t-dna. we present evidence that the ac element transposes in transformed root or root-derived callus cultures of both species. we show that fertile plants can be regenerated from transformed, root-derived callus cultures of arabidopsis, demonstrating the utility of the ri plasmid fo ... | 1987 | 2832144 |
| isolation of genes expressed in specific tissues of arabidopsis thaliana by differential screening of a genomic library. | the small genome size of arabidopsis thaliana allows the isolation of genes expressed in specific tissues and under controlled conditions by the differential screening of a genomic library, as has been shown previously for yeast and drosophila. cdna probes, based on poly(a)+ mrna isolated from different arabidopsis organs, were used in colony hybridizations with 1145 randomly chosen genomic clones, representing 27,000 kb of arabidopsis dna. twenty percent of the clones containing low-copy-number ... | 1988 | 2901388 |
| conversion of perianth into reproductive organs by ectopic expression of the tobacco floral homeotic gene nag1. | mutations in the agamous (ag) gene of arabidopsis thaliana result in the conversion of reproductive organs, stamens and carpels, into perianth organs, sepals and petals. we have isolated and characterized the putative ag gene from nicotiana tabacum, nag1, whose deduced protein product shares 73% identical amino acid residues with the arabidopsis ag gene product. rna tissue in situ hybridizations show that nag1 rna accumulates early in tobacco flower development in the region of the floral merist ... | 1993 | 7507255 |
| two related low-temperature-inducible genes of arabidopsis encode proteins showing high homology to 14-3-3 proteins, a family of putative kinase regulators. | we have isolated two rare cold-inducible (rci1 and rci2) cdnas by screening a cdna library prepared from cold-acclimated etiolated seedlings of arabidopsis thaliana with a subtracted probe. rna-blot hybridizations revealed that the expression of both rci1 and rci2 genes is induced by low temperature independently of the plant organ or the developmental stage considered. however, rci1 mrna accumulates faster and at higher levels than the rci2 one indicating that these genes have differential resp ... | 1994 | 7520301 |
| role of the phytotoxin coronatine in the infection of arabidopsis thaliana by pseudomonas syringae pv. tomato. | the role of the phytotoxin coronatine in the virulence of pseudomonas syringae pv. tomato in arabidopsis thaliana was evaluated by comparing symptom development, in planta bacterial multiplication, and the induction of defense-related genes in arabidopsis plants inoculated with the coronatine-producing (cor+) p. s. pv. tomato strain dc3000 and the coronatine-defective (cor-) strain dc3661 by either infiltration or dipping methods. the cor+ strain, p. s. pv. tomato dc3000, caused severe disease s ... | 1995 | 7539639 |
| an upstream u-snrna gene-like promoter is required for transcription of the arabidopsis thaliana 7sl rna gene. | the genes transcribed by rna polymerase (pol) iii can be placed into four distinct groups based on the nature and position of their promoter elements. in the higher eukaryotes equivalent genes usually belong to the same sub-type of pol iii promoters and there are few examples of genes which have changed promoter type during evolution. in this work we demonstrate that the promoter of the arabidopsis thaliana 7sl rna gene is located upstream of the coding region and is identical to the promoters o ... | 1995 | 7541131 |
| the gene and the rna for the precursor to the plastid-located glycerol-3-phosphate acyltransferase of arabidopsis thaliana. | the gene and the rna from arabidopsis thaliana for the plastid-located glycerol-3-phosphate acyltransferase (gpat; ec 2.3.1.15) and their encoded product have been studied. the gene (designated ats1) was isolated by screening a lambda dash genomic library for cross-hybridization with a radiolabeled probe prepared from cdna for gpat from squash. cdna clones representing the mrna were isolated by screening a lambda zapii cdna library for hybridization with a radiolabeled probe prepared from a dna ... | 1993 | 7678766 |
| an arabidopsis mutant deficient in sterol biosynthesis: heterologous complementation by erg 3 encoding a delta 7-sterol-c-5-desaturase from yeast. | the mutant ste 1 was isolated by screening an ethylmethane sulfonate (ems)-mutagenized population of arabidopsis thaliana which consisted of 22,000 m2 plants divided into 1100 pools of 20 plants by gas chromatography of sterols extracted from small leaf samples. ste 1 was characterized by the accumulation of three delta 7-sterols concomitantly with the decrease of the three corresponding delta 5-sterols which are the end products of the sterol pathway in wild-type leaves. the structure of these ... | 1995 | 7550378 |
| genetic ablation of petal and stamen primordia to elucidate cell interactions during floral development. | two models have been proposed to explain the coordinated development of the four whorls of floral organs. the spatial model predicts that positional information defines the four whorls simultaneously, and that individual organs develop independently of surrounding tissues. the sequential model suggests that inductive events between the outer and inner whorl primordia are required for appropriate organogenesis. to test these models we have genetically ablated second and third whorl floral organ p ... | 1995 | 7555715 |
| physical map and organization of arabidopsis thaliana chromosome 4. | a physical map of arabidopsis thaliana chromosome 4 was constructed in yeast artificial chromosome clones and used to analyze the organization of the chromosome. mapping of the nucleolar organizing region and the centromere integrated the physical and cytogenetic maps. detailed comparison of physical with genetic distances showed that the frequency of recombination varied substantially, with relative hot and cold spots occurring along the whole chromosome. eight repeated dna sequence families we ... | 1995 | 7570002 |
| a new member of the cytosolic o-acetylserine(thiol)lyase gene family in arabidopsis thaliana. | a cdna, atcys-3a, encoding o-acetylserine-(thiol)lyase has been isolated from arabidopsis thaliana. the deduced peptide sequence showed a high level of similarity with the bacterial counterpart, and a remarkable percentage of identity with other higher plant o-acetylserine(thiol)lyase genes. sequence comparison and southern blot analysis suggested that atcys-3a was a new and different to the previously reported member of the cytosolic gene family in arabidopsis. the atcys-3a expression was activ ... | 1995 | 7729527 |
| the mitochondrial pyruvate dehydrogenase complex: nucleotide and deduced amino-acid sequences of a cdna encoding the arabidopsis thaliana e1 alpha-subunit. | a cdna encoding the e1 alpha subunit of the arabidopsis thaliana (at) mitochondrial (mt) pyruvate dehydrogenase complex (pdc) was sequenced. the 1435-bp cdna consists of a 1167-bp open reading frame encoding a 43.0-kda polypeptide of 389 amino acids (aa) (pi 7.1). the plant e1 alpha subunit has 47-51% aa sequence identity with other eukaryotic sequences. among the regions that are highly conserved are the aa surrounding phosphorylation sites 1 and 2 of the mammalian sequence, including the conse ... | 1995 | 7590338 |