Publications

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comparing low coverage random shotgun sequence data from brassica oleracea and oryza sativa genome sequence for their ability to add to the annotation of arabidopsis thaliana.since the completion of the arabidopsis thaliana genome sequence, there is an ongoing effort to annotate the genome as accurately as possible. comparing genome sequences of related species complements the current annotation strategies by identifying genes and improving gene structure. a total of 595,321 brassica oleracea shotgun reads were sequenced by tigr (the institute for genome research) and the collaboration of washington university and cold spring harbor. vicogenta (a genome viewer based ...015805491
transcription factor families have much higher expansion rates in plants than in animals.transcription factors (tfs), which are central to the regulation of gene expression, are usually members of multigene families. in plants, they are involved in diverse processes such as developmental control and elicitation of defense and stress responses. to investigate if differences exist in the expansion patterns of tf gene families between plants and other eukaryotes, we first used arabidopsis (arabidopsis thaliana) tfs to identify tf dna-binding domains. these dna-binding domains were then ...016166257
nuclear dna amounts in angiosperms: progress, problems and prospects.the nuclear dna amount in an unreplicated haploid chromosome complement (1c-value) is a key diversity character with many uses. angiosperm c-values have been listed for reference purposes since 1976, and pooled in an electronic database since 1997 (http://www.kew.org/cval/homepage). such lists are cited frequently and provide data for many comparative studies. the last compilation was published in 2000, so a further supplementary list is timely to monitor progress against targets set at the firs ...015596457
switch1 (swi1): a novel protein required for the establishment of sister chromatid cohesion and for bivalent formation at meiosis.we have characterized a new gene, swi1, involved in sister chromatid cohesion during both male and female meiosis in arabidopsis thaliana. a first allele, swi1.1, was obtained as a t-dna tagged mutant and was described previously as abnormal exclusively in female meiosis. we have isolated a new allele, swi1.2, which is defective for both male and female meiosis. in swi1.2 male meiosis, the classical steps of prophase were not observed, especially because homologs do not synapse. chromatid arms a ...011459834
biochemical mutations in the crucifer arabidopsis thaliana (l.) heynh. 195513244676
[case of superdominance of experimentally produced autotetraploids of arabidopsis thaliana]. 195513312459
[the influence of ionizing radiations on the morphogenesis of embryonal organs. i. embryo germ anomalies caused by roentgen irradiation of arabidopsis thaliana embryo in various developmental stages]. 195914437191
[studies on the development of submicroscopic chloroplast structure in leaf pigment mutants of arabidopsis thaliana]. 195914438263
effect of thymidine analogues on reproductive morphogenesis in arabidopsis thaliana (l.) heynh. 196214015980
chromosome studies in arabidopsis thaliana. 196317248159
isolation of nutritional mutants in arabidopsis thaliana. 19645849557
genetic effects of ethyl methanesulfonate in combination with copper and zinc ions on arabidopsis thaliana. 19655863906
[disturbed thylakoid-formation in chloroplasts of a xantha-mutant of arabidopsis thaliana (l.) heynh].a monohybrid recessive xantha-mutant of arabidopsis thaliana was grown aseptically on mineral agar +2% sucrose up to maturity. the cotyledons and rosette leaves were non-green during all developmental stages, their yellow colour bleaching with age.palisade cells of various leaves of mutant plants were investigated light- and electron microscopically. all of the organelles of these cells, except the plastids, had a normal appearance. special attention was paid to the existence of "dense bodies", ...196624557822
[relations between chlorophyll content and chloroplast fine structure in a chlorina mutant of arabidopsis thaliana (l.) heynh].in a chlorina mutant (ch 3) of arabidopsis thaliana the total chlorophyll content was reduced to about 1/10 the amount of the green wild-type. at the same time the ratio of chlorophyll a/b was markedly increased. the electron microscopic analysis of the chloroplasts showed a considerable reduction in the membrane formation. especially an inhibition of the grana differentiation was apparent, leading in some stages to an entirely abnormal thylakoid stacking. depending on developmental as well as e ...196624557719
[activation of thymidine-3h incorporation in the desoxyribonucleic acid of arabidopsis thaliana l. heynh. induced by gamma radiations]. 19664168223
killing and mutagenic efficiencies of heavy ionizing particles in arabidopsis thaliana. 19676030572
radiation-induced polygenic mutation in arabidopsis thaliana. ii. analysis of lines selected for flowering time. 19685254096
[fate of exogenous dna during the growth and development of "arabidopsis thaliana"]. 19694181702
[redistribution, at the time of blossoming, of exogenous dna absorbed by arabidopsis thaliana seeds]. 19694186392
gene locus specificity of the glucose effect in the thiamine pathway of the angiosperm, arabidopsis.all mutants at 3 loci in arabidopsis thaliana (l.) heynh., a higher plant, that are associated with the synthesis or coupling of the thiazole moiety of thiamine are susceptible to reversible glucose inhibition. in contrast, several different alleles involved in the synthesis of the pyrimidine moiety of the vitamin are insensitive to glucose. glucose and maltose are equally effective inhibitors while fructose, lactose, ribose, and xylose are toxic. this toxicity is not released by added thiamine.196916657050
[study of descendants of "arabidopsis thaliana" plants treated with bacterial dna. i. presence and replication of exogenous dna]. 19704098023
[mutagenic effect of laser radiation on the seeds of arabidopsis thaliana (l.) heynh]. 19705474214
fate of exogenous dna in arabidopsis thaliana. translocation and integration. 19714942550
effect of temperature on survival of gamma-irradiated seeds of arabidopsis thaliana. 19715549253
photomorphogenesis in arabidopsis thaliana (l.) heynh: threshold intensities and blue-far-red synergism in floral induction.arabidopsis seeds were germinated on sterile mineral agar supplemented with 1% glucose and cultured under continuous light regimes. with 4-hour incandescent plus 20-hour monochromatic illumination in the region from 400 to 485 nanometers there was effective floral induction at an intensity of 100 microwatts per square centimeter. exclusion of far red wave lengths from the 4-hour incandescent period sharply reduced the effectiveness of subsequent monochromatic blue light in promoting floral induc ...197116657629
[physiological genetics of quantitative characters in arabidopsis thaliana (l.) heynh. : part 3. dauermodifications of pleiotropic effects of homoallelic genes].the genephysiological and developmental basis of "dauermodifications" (dm) have still not been explained. in particular definite evidence for dm in plastids is lacking up to now. moreover no attention has been paid to the connections with the problem of quantitative gene effects. for that reason mutants of the nuclear gene ch in arabidopsis thaliana (l.) heynh., quantitatively defective in chlorophyll b were tested during seven generations under continuous yellow radiation (λ < 550 nm) expecting ...197124430242
[physiological genetics of quantitative characters in arabidopsis thaliana (l.) heynh. : part 2: modification of primary and secondary genetic effects of single-gene chlorophyll-b mutants by long-wave irradiation].an analysis of the quantitative molecular effects of genes requires information on the norm of reaction ("reaktionsnorm") and on its variability in the genotype tested. 1) experiments were carried out in an automatically controlled growth chamber to determine the optimal conditions for the norm of reaction of the plants to be tested. genotypically determined variances between the characters studied and differences of means between test samples were most frequently significant when the growth con ...197124430106
[physiological genetics of quantitative characters in arabidopsis thaliana (l.) heynh. : part 1: segregation and biosynthesis of pigments in chlorophyll-b defect mutants].in biometric studies on the genetics of quantitative characters the problem of regulation of the activity of genes is rarely considered. mutants of arabidopsis thaliana (l.) heynh. (cruciferae), defective for chlorophyll b, permit a direct biochemical and physiological determination of their quantitative gene effects in the biosynthetic pathway of the plastid pigments. in agreement with other authors, the detectable genotypes are found to be based on multiple alleles (ch (+), ch (1) and ch (2)) ...197124430018
development and differentiation of haploid lycopersicon esculentum (tomato).haploid callus cultures of selected races of lycopersicon (tomato) species can be obtained from anther culture. this is a further demonstration of a proposed general method of haploid culture developed with arabidopsis thaliana. differentiation of haploid callus of lycopersicon esculentum can be controlled both in the dark and the light by hormones added to defined minimal media. development to plantlets is achieved only in the light. callus cells can be induced to develop into seedless pseudo-f ...197224477400
selection for fresh weight in arabidopsis thaliana under two mating systems.four races of arabidopsis thaliana were used to produce two f 2 populations and these constituted the base populations of an artifical selection study. all plants were grown under aseptic culture conditions in a controlled environment and the character studied was fresh weight after 15 days' growth. for one section of the study, two selection intensities were applied to each f 2 population and the selected parents were randomly mated. in addition to the high selections a group of random selectio ...197224430772
effects of space flight factors on the heredity of higher and lower plants.the effects were studied of a long-term space exposure (72 days) aboard the salyut orbital station on the following: dry seeds of crepis capillaris (l.) wallr. and arabidopsis thaliana (l.) heynh, haploid and diploid strains of saccharomyces cerevisiae mutant for adenine locus (ad) and strain larg-i of chlorella vulgaris beijer. a modifying effect of space factors on radiobiological action of gamma-irradiation from 137cs was determined with the higher plant seeds and chlorella culture. for this, ...197311998855
the biostack experiment on apollo 16.the object of the biostack experiment is to study the biological effects of high ze particles of cosmic radiation in order to obtain information on the mechanism of these particles in biological matter. for this purpose individual local evaluation methods have been developed which allow one to identify each biologically effective particle and to correlate the individual hitting particle with the biological effect produced. the biostack experimental package contains a series of monolayers of sele ...197312001958
biological and molecular evidence for the transgenosis of genes from bacteria to plant cells.specialized transducing phages (lambda and varphi80) have been used as vectors in the transfer of genes (wild type and mutant) from the bacterium escherichia coli to haploid cell lines of the plants lycopersicon esculentum and arabidopsis thaliana. the overall phenomenon of transfer, gene maintenance, transcription, translation, and function has been termed transgenosis. transgenosis of galactose and lactose operon genes was detected by survival and growth of the plant cells on defined medium wi ...197316592069
dna-mediated transformation of arabidopsis thaliana. 19734133555
dna-mediated genetic correction of thiamineless arabidopsis thaliana. 19744833229
radiobiological results of the biostack experiment on board apollo 16 and 17.after penetrating the biostack capsule, some of the hze particles hit the biological objects carried: bacterial spores (bacillus subtilis), seeds (arabidopsis thaliana and vicia faba), and shrimp eggs (artemia salina). the different biological objects were affected by heavy ions in widely varying ways. a broad range of radiobiological investigations has been carried out in regard to the objects' response to hze particles. the most sensitive biological objects in the biostack experiments proved t ...197511913420
the biological effectiveness of hze-particles of cosmic radiation studied in the apollo 16 and 17 biostack experiments.the biostack experiments i and ii were flown on board the apollo 16 and 17 command modules in order to obtain information on the biological damage produced by the bombardment of heavy high-energy (hze) particles of cosmic radiation during spaceflight. such data are required for estimating radiation hazards in manned spaceflight. seven biological systems in resting state (bacillus subtilis spores, colpoda cucullus cysts, arabidopsis thaliana seeds, and eggs of artemia salina, tribolium castaneum ...197511887916
quantitative dose-response of growth and development in arabidopsis thaliana exposed to chronic gamma-radiation.the response of arabidopsis thaliana (l.) heynh. (cruciferae) to a gradient of chronic gamma-radiation was examined under field conditions. plants that were initially introduced to the gamma field as dry seeds received exposures of 1-34 to 18 800 r/20 hour day from the time of seed germination. regression analysis demonstrated a significant, but non-linear, response for three variables, number of seedlings emerging, number of plants flowering, and plant volume; the response of a fourth variable, ...19751081091
genetic control of acid phosphate isozymes in arabidopsis thaliana (l.) heynh.starch gel electrophoresis in a discontinuous buffer system has enabled us to study two acid phosphatase systems (acph-1 and acph-2) in arabidopsis thaliana. the acph-2 bands are much more prominent than those belonging to acph-1. among the 17 geographic races, one variant for acph-2 was detected. a study of f1 hybrids and f2 segregating progenies showed that acph-2 variants are controlled by two codominant alleles (acph-2n and acph-2f) at one locus. the heterozygotes are characterized by an int ...19751203053
maternal and reciprocal effects on seedling characters in arabidopsis thaliana (l.) heynh.five early growth characters were examined in six races of arabidopsis thaliana (l.) heynh, their reciprocal f(1) hybrids (1974) and f(1) by tester hybrids, using a seventh race as a paternal tester. three of the five characters were also examined at two nutrient levels in reciprocal f(1) hybrids (1972) of all seven races. analyses of f(1) and f(1) by tester hybrids revealed significant maternal effects in all characters examined in f(1) hybrids (1972) and in root length and plant weight of f(1) ...197617248705
morphology of arabidopsis grown under chronic centrifugation and on the clinostat.morphological measurements were made on populations of arabidopsis thaliana grown from seed for 21 days under essentially constant environmental conditions except for the influence of gravitational or centrifugal accelerations. growth conditions were what had been proposed for experiments in an artificial satellite. observations are reported for plants grown at normal 1-g upright or on horizontal clinostats and for plants grown on a centrifuge. increased g-force, up to 15 times normal, was found ...197616659483
biological investigations of higher and lower plants aboard soyuz 19.biological investigations carried out aboard soyuz 19 consisted of three independent complex experiments. all biological materials aboard the spacecraft were kept in "biocat" thermostats which maintained the required temperature. the objective of the experiment "genetic investigations" was to determine the effect of space flight factors on the heredity of lower (chlamydomonas reinhardi dang) and higher (crepis capillaris (l) wallr and arabidopsis thaliana (l) heynh) plants, and on the radiosensi ...197711958205
somatic mosaicism in plants with special reference to somatic crossing over.plant systems in use for the detection of environmental mutagens appear capable of detecting all types of genetic effects which can be studied in animals. the study of somatic mosaicism, however, is better developed in plants than in higher animals. a case is presented here which shows the ability of plant systems in analyzing a host of genetic end points, including chromosome aberrations like deletions, somatic crossing over, numerical inequality, gene conversion, paramutations and point mutati ...1978367771
selection and characterization of cell lines of arabidopsis thaliana resistant to amino acid analogs [proceedings]. 197881019
glutamate dehydrogenase in arabidopsis thaliana: polymorphism properties and effect of nitrogen source on enzyme activity [proceedings]. 197881014
[growth of arabidopsis thaliana embryos following irradiation of the plants during the generative phase].the flowers and young legumes of arabidopsis thaliana (phases of development b3--b7) were irradiated by gamma-rays at the dose of 4 kr. the irradiation did not influence the duration of phases of the flower development irrespective of the phase of irradiation. the cytological characteristics of embryos is given for each phase under study. the duration of developmental stages (from zygote till the spheroid stage) in the control and following the irradiation was practically the same. the irradiati ...1978714364
biological investigations on the orbital station "salyut-5".genetic and embryological investigations were continued on the orbital station salyut-5. the effect of space flight factors on dry crepis capillaris (l) wallr seeds and seedlings, dry arabidopsis thaliana (l) heynh seeds, polyporus brumalis pers. ex. fries culture as well as on spawn development and fish behaviour was studied. space flight factors are shown to have adverse effects with respect to some characteristics in seeds and seedlings, and to change the morphology of the fungus. fishes appe ...197912008713
serendipitous solution to the problem of culturing arabidopsis plants in sealed containers for spaceflights of long duration.for the development of an experiment to determine how a higher plant will develop from seed to maturity when deprived of essentially all gravitational information that it might use to guide its growth, we selected arabidopsis thaliana as our test species. we were unable to culture it heterophically on nutrient agar in darkness. light was required for germination and growth. presumably the plants were dependent on photosynthesis for the major contribution to their organic nutrition. plants were g ...197912269362
the action of space flight factors on the radiation effects of additional gamma-irradiation of seeds.the action of space flight factors (sff) on radiosensitivity and the effects of preflight irradiation of air-dried crepis capillaris (l) wallr and arabidopsis thaliana (l) heynh seeds aboard the spaceships of the cosmos, soyuz and salyut series was studied. the results have shown that vibration and linear acceleration (va) cause damage of the meristematic embryonic cells and simultaneously decrease the radiosensitivity of seeds. sff enhance the effect of preflight gamma-irradiation of seeds with ...197912008699
isolation and characterization of six embryo-lethal mutants of arabidopsis thaliana. 1979510781
embryo-lethal mutants of arabidopsis thaliana. a model system for genetic analysis of plant embryo development. 1979510780
variations among races of arabidopsis thaliana (l.) heynh for survival in limited carbon dioxide.races of a c3 plant species, arabidopsis thaliana, were screened for time of survival when enclosed in an air-tight chamber with a c4 plant species (zea mays l.). this has been suggested as a method of detecting and selecting for increased photosynthetic efficiency among c3 genotypes. the c4 plant should, in such conditions, reduce the atmospheric co2 concentration below the compensation point of the c3 plant, resulting in its eventual death. significant differences were found among the a. thali ...197924310038
rubidium (potassium) uptake by arabidopsis: a comparison of uptake by cells in suspension culture and by roots of intact seedlings.experiments are reported in which the uptake of (86)rb(+), used as an analog of k(+), into cultured cells of arabidopsis thaliana is investigated. a single transport system is found with k(m) = 0.34 millimolar and v(max) = 14 nmoles per milligram of protein per hour. this system is blocked by the metabolic inhibitor carbonyl cyanide m-chlorophenyl hydrazone (cccp) and by cold. at high concentrations of external k(+) (above 1 millimolar), a significant fraction of total uptake is energy-independe ...197916660969
"arabidobrassica": evidence for intergeneric somatic hybrid nature from electron microscopic morphometry of chromatin.the new method of species and hybrid characterization and determination by quantitative morphometry of chromatin texture in ultra-thin sections of nuclei was applied to the somatic hybrid "arabidobrassica" and its parents, arabidopsis thaliana and brassica campestris. the somatic hybrid exhibits the average amount of condensed chromatin, if compared to the proportion of condensed chromatin in nuclei of the parent species. this result corresponds to similar findings in natural allopolyploids and ...19807398664
mutants of arabidopsis thaliana with altered responses to auxins and gravity.auxin-resistant mutants of arabidopsis have been induced and isolated by screening for survivors on a medium containing the herbicide 2,4-d. thirty independently arisen mutants have been isolated in this way and one of them, p 83, has been investigated in detail. when wild type and p 83 are compared in concentration/response curves, where the response is the inhibition of root growth, the ed50 values of the auxins, 2,4-d and iaa, are 14-fold higher for the mutant. the mutant also responds differ ...19807247923
induction and analysis of gibberellin sensitive mutants in arabidopsis thaliana (l.) heynh.in arabidopsis thaliana 37 independent irradiation or ems induced mutants were isolated which have an absolute or almost absolute gibberellin (ga) requirement for germination and successive elongation growth. these are called 'non-germinating ga-dwarfs', since without further addition of ga they develop into typical ga-dwarfs, being dark green, stunted and sterile. however, with repeated ga-treatment they develop into fertile plants with a completely wild type phenotype, or nearly so. in additio ...198024301503
"arabidobrassica": a novel plant obtained by protoplast fusion.using somatic hybrid cell lines arabidopsis thaliana+brassica campestris, obtained by cloning individual protoplast-fusion products as starting material, shoots and flowering plants have been regenerated. cytological, biochemical, and morphological analyses indicate that genetic material of both species is present in the resultant plants. shoots and plants obtained from different lines and different regeneration events differed morphologically and genetically. most regenerants show morphological ...198024306240
segregation frequencies of radiation-induced viable mutants in arabidopsis thaliana (l.) heynh.the segregation of viable mutants derived from various fast neutron and x-ray treatments of arabidopsis seeds was studied in the m2- and m3-generation. an equal segregation frequency in the m2- and m3-generation was observed. this indicated that the m2-lines, each of which descended from a single silique from the top of the main inflorescence of an m1-plant, originated from non-chimeric tissue. furthermore, it was found that neither radiation type nor radiation dose affected the segregation freq ...198024302496
photorespiration mutants of arabidopsis thaliana deficient in serine-glyoxylate aminotransferase activity.three mutants of the crucifer arabidopsis thaliana (linnaeus) heynhold were isolated that are completely lacking in activity catalyzed by serine-glyoxylate aminotransferase (ec 2.6.1.45), a peroxisomal enzyme involved in photorespiratory carbon metabolism. these mutants were viable and exhibited normal photosynthesis under conditions that suppressed photorespiration, but they were inviable and photosynthesized at greatly reduced rates under conditions that promoted photorespiration. serine and g ...198016592821
[structural and functional characteristics of the photosynthetic apparatus of the mutants arabidopsis thaliana (l.) heynh].the mutants arabidopsis thaliana triplex and 58/15 with a high and low content of green pigments, respectively, and a higher rate of photosynthesis in comparison with the control form of enkheim were studied. the significant differences in the structural organization of chloroplasts of both mutants were revealed. the two mutants are also characterized by a higher rate of ferricyanide reduction in the hill reaction and atp production via photophosphorylation. it was shown that mg2+, nh4cl and (ad ...19807378506
biological investigations aboard the biosatellite cosmos-1129.experiments on insects, higher plants and lower fungi were carried out aboard the biological satellite cosmos-l129, in earth orbit, from 25 september to 14 october 1979. the main objective of these experiments was to gain more profound knowledge of the effect of weightlessness on living organisms and to study the mechanisms by which these various organisms with different life cycles can adjust and develop in weightlessness. experiments on insects (drosophila melanogaster) were made with a view t ...198111541724
"arabidobrassica": chromosomal recombination and morphogenesis in asymmetric intergeneric hybrid cells.a somatic hybrid cell line, cloned from an individual protoplast-fusion product between arabidopsis thaliana and brassica campestris, gave rise to formation of numerous plants differing drastically in morphology. analysis of these various regenerants, all of which originated from one and the same heterokaryon derived from the fusion of two cells, shows the unspecific elimination of chromosomes of both parental species during the callus growth phase. whereas the parental cells have so far not bee ...198124275880
fraction i protein analysis of parasexual hybrid plants arabidopsis thaliana + brassica campestris.the polypeptide composition of fraction i protein (ribulose-1,5-bisphosphate carboxylase) prepared from leaves of two clones of the parasexual hybrid plant arabidopsis thaliana + brassica campestris as well as their parents was analyzed by isoelectric focusing. the protein in hybrid plants contained a heterogenous population of small subunits resulting from the expression of both arbabidopsis and brassica nuclear genes, whereas the large subunit polypeptides, and hence the functional chloroplast ...198124258862
photorespiration-deficient mutants of arabidopsis thaliana lacking mitochondrial serine transhydroxymethylase activity.three allelic mutants of arabidopsis thaliana which lack mitochondrial serine transhydroxymethylase activity due to a recessive nuclear mutation have been characterized. the mutants were shown to be deficient both in glycine decarboxylation and in the conversion of glycine to serine. glycine accumulated as an end product of photosynthesis in the mutants, largely at the expense of serine, starch, and sucrose formation. the mutants photorespired co(2) at low rates in the light, but this evolution ...198116661733
the role of weightlessness in the genetic damage from preflight gamma-irradiation of organisms in experiments aboard the salyut 6 orbital station.the effect of weightlessness on chromosomal aberration frequency in preflight irradiated crepis capillaris seeds, on the viability, fertility and mutation frequency in arabidopsis thaliana, and on the frequency of nondisjunction and loss of x chromosomes in pre-flight irradiated drosophila melanogaster gametes was studied aboard the salyut 6 orbital station. the following effects were observed: a flight-time dependent amplification of the effects of preflight gamma-irradiation in a. thaliana wit ...198111541706
mutants of the cruciferous plant arabidopsis thaliana lacking glycine decarboxylase activity.a mutant of arabidopsis thaliana (l.) heyn. (a small plant in the crucifer family) that lacks glycine decarboxylase activity owing to a recessive nuclear mutation has been isolated on the basis of a growth requirement for high concentrations of atmospheric co2. mitochondria isolated from leaves of the mutant did not exhibit glycine-dependent o2 consumption, did not release 14co2 from [14c]glycine, and did not catalyse the glycine-bicarbonate exchange reaction that is considered to be the first p ...19826807291
embryo-lethal mutants of arabidopsis thaliana: evidence for gametophytic expression of the mutant genes.normal and aborted seeds from two recessive embryo-lethal mutants (79a and 124d) of arabidopsis thaliana were shown to be distributed nonrandomly along the length of heterozygous siliques. significantly more than half of the aborted seeds in these two mutants were located in the top half of the silique, in the region closest to the stigma surface. segregation ratios (percent aborted seeds) were unusually low at the base of the silique, and slightly higher than expected at the tip. in contrast, a ...198224270876
the isolation of abscisic acid (aba) deficient mutants by selection of induced revertants in non-germinating gibberellin sensitive lines of arabidopsis thaliana (l.) heynh.by selecting for germinating seeds in the progeny of mutagen-treated non-germinating gibberellin responsive dwarf mutants of the ga-1 locus in arabidopsis thaliana, germinating lines (revertants) could be isolated. about half of the revertants were homozygous recessive for a gene (aba), which probably regulates the presence of abscisic acid (aba). arguments for the function of this gene were obtained from lines homozygous recessive for this locus only, obtained by selection from the f2 progeny o ...198224270501
reverse mutants of the nitrate reductase-deficient mutant b 25 of arabidopsis thaliana.revertants of b25, a nitrate reductase-deficient mutant of arabidopsis thaliana (l.) heinh, were isolated with a high frequency. all 7 independently arisen revertants were mutations in the same suppressor gene su, which is unlinked to the originally mutated gene rgn. the mutant character shows up both in growth on nitrate as the sole nitrogen source and in susceptibility to chlorate. when judged for these properties the mutant alleles are either dominant for both, recessive for both or dominant ...198224270438
a mutant of arabidopsis thaliana which lacks activation of rubp carboxylase in vivo.a mutant of arabidopsis thaliana has been isolated in which ribulose-1,5-bisphosphate carboxylase is present in a nonactivatable form in vivo. the mutation appears to affect carboxylase activation specifically, and not any other enzyme of the photosynthesis or photorespiratory cycles. the effect of the mutation on carboxylase activation is indirect, inasmuch as the properties of ribulose-1,5-bisphosphate carboxylase purified from the mutant are not distinguishable from those of the wild type enz ...198216662500
isolation and characterization of nitrate reductase-deficient mutants of arabidopsis thaliana.chlorate resistant mutants of arabidopsis thaliana were isolated, of which 10 exhibited a lowered nitrate reductase activity and 51 were chlorate-resistant because of an impaired uptake of chlorate. the 51 mutants of this type are all affected in the same gene. the mutants with a lowered nitrate reductase activity fall into 7 different complementation groups. three of these mutants grow poorly on media with nitrate as the sole nitrogen source, while the others apparently can reduce sufficient ni ...198224264829
ems- and radiation-induced mutation frequencies at individual loci in arabidopsis thaliana (l.) heynh.average mutation frequencies per locus per dose of mutagen - ems, x-rays and fast neutrons - were estimated from 15 different loci of arabidopsis thaliana. these loci relate to clearly distinguishable phenotypic mutant groups, each affected by a limited number of loci. there were significant differences in mutation rate between individual loci. the proposition of a linear relationship between dna content and mutation frequency per locus is rejected.19827062928
the positive hook: the role of gravity in the formation and opening of the apical hook.photographic observations on germinating seedlings of lepidium sativum l., cucumis sativus l., and helianthus annuus l. showed that the hypocotyl hook is not present in the seed but forms during the early stages of growth. evidence that gravity plays a major role in inducing curvature of the hypocotyl, and in maintaining the hook once it has been formed, was obtained from clinostat experiments, from the use of morphactin to remove geotropic sensitivity and from inversion of seedlings to change t ...198324264451
radiation induced developmental instability in arabidopsis thaliana.in a study on the nature of induced genetic variations in quantitative characters using the selfpollinated plant arabidopsis thaliana, it was observed that not only quantitative performance but also intraline variability, or developmental instability, was affected by induced mutations. emphasis was placed on the latter.seeds of landsberg strain were used. two irradiated (20 kr and 80 kr) populations together with a control population were propagated by self-fertilization for six generations usin ...198324263206
induction of dormancy during seed development by endogenous abscisic acid: studies on abscisic acid deficient genotypes of arabidopsis thaliana (l.) heynh.mutant lines of arabidopsis thaliana (l.) heynh., which are characterized by symptoms of withering and the absence of seed dormancy, showed much lower levels of endogenous abscisic acid (aba) in developing seeds and fruits (siliquae) than the wild type. reciprocal crosses of wild type and aba-deficient mutants showed a dual origin of aba in developing seeds. the genotype of the mother plant regulated a sharp rise in aba content half-way seed development (maternal aba). the genotype of the embryo ...198324264070
an arabidopsis thaliana mutant defective in chloroplast dicarboxylate transport.reactions of the photorespiratory pathway of c(3) plants are found in three subcellular organelles. transport processes are, therefore, particularly important for maintaining the uninterrupted flow of carbon through this pathway. we describe here the isolation and characterization of a photorespiratory mutant of arabidopsis thaliana defective in chloroplast dicarboxylate transport. genetic analysis indicates the defect is due to a simple, recessive, nuclear mutation. glutamine and inorganic phos ...198316593285
chlorophyll-protein-complexes of thylakoids of wild type and chlorophyll b mutants of arabidopsis thaliana.pigment-protein-complexes of two chlorophyll b deficient mutants of arabidopsis and from the wild type were separated electrophoretically. light-harvesting proteins were absent in the chlorophyll b free mutant ch(1) and their amount was reduced in the mutant ch(2) which has a reduced content of chlorophyll b. the ratio of cpa:cp i increased with decreasing chlorophyll b content which indicated that the stoichiometry of photosystem ii to photosystem i is not constant.198324458410
experiments with air-dried seeds of arabidopsis thaliana (l) heynh. and crepis capillaris (l) wallr., aboard salyut 6.space flight factors resulted in the accumulation of genetic damage in embryonic meristem cells of seeds of arabidopsis thaliana and crepis capillaris in flights of different duration (49, 226, 408 and 827 days) aboard the orbital station salyut 6. as a result, the viability of seeds and seedlings was reduced, and the sterility of plants grown from seeds exposed on salyut 6 was increased. the effect depended upon the flight duration. the data obtained suggest an acceleration of seed aging under ...198311542740
peculiarities of genital organ formation in arabidopsis thaliana (l) heynh. under spaceflight conditions.an experiment was carried out ahoard the salyut 6 research orbital station on arabidopsis thaliana cultivations. the seeds were sprouted in the svetoblok 1 device which provides for plant growth in the agar medium under sterile conditions and at 4000 lux illumination. the experimental plants, as well as the controls, reached approximately the same developmental stages: both flowered and began to bear fruit. a microscopic examination of the generative organs in the control and experimental plants ...198311542455
chlorophyll-protein-complexes of thylakoids of wild type and chlorophyll b mutants of arabidopsis thaliana.pigment-protein-complexes of two chlorophyll b deficient mutants of arabidopsis and from the wild type were separated electrophoretically. light-harvesting proteins were absent in the chlorophyll b free mutant ch(1) and their amount was reduced in the mutant ch(2) which has a reduced content of chlorophyll b. the ratio of cpa:cp i increased with decreasing chlorophyll b content which indicated that the stoichiometry of photosystem ii to photosystem i is not constant.198324458523
thermal adaptation and acclimation of higher plants at the enzyme level: kinetic properties of nad malate dehydrogenase and glutamate oxaloacetate transaminase in two genotypes of arabidopsis thaliana (brassicaceae).kinetic properties of nad malate dehydrogenase (mdh) and glutamate oxaloacetate transaminase (got) were analyzed in two genotypes of arabidopsis thaliana collected in two sites of contrasting climates. plants from each genotype were acclimated under controlled conditions at four different thermoperiods: 5-10° c, 7-15° c, 15-25° c and 25-28° c. apparent energy of activation for mdh of the cold adapted genotype were significantly lower at low temperatures of acclimation, while for got, significant ...198328310480
polymorphism of alcohol dehydrogenase in arabidopsis thaliana (l.) heynh.: genetical and biochemical characterization.zymograms of arabidopsis alcohol dehydrogenase (adh; ec 1.1.1.1) show a unique anodal migrating band. three electrophoretic variants were identified among geographical races and designated slow (s), fast (f), and superfast (a), according to their mobility on tris-citrate starch gels. in plants adh activity is confined mainly to pollen, seeds, and grains and rapidly declines during the germination process. in callus and suspension cultures, growing on media containing 2,4-d, adh appeared as one o ...19846393951
radiosensibility of higher plant seeds after space flight.the influence of long-term storage of higher plant seeds under space flight conditions (49 to 827 days) on their radiosensibility was studied in the experiments on the orbital stations salyut 6 and 7. short-term storage has been proved to have no effect on radiosensitivity of crepis capillaris seeds. only in the case of maximal exposure duration the frequency of chromosome aberrations in post-flight irradiated seeds significantly exceeded the chromosome aberration frequency in the ground-based i ...198411539615
arabidopsis seed production limited by co2 in simulated space experiments.arabidopsis thaliana (l.) henyh. plants were successfully grown many times in simulated space experiments from seed to seed in our laboratory. attempts were unsuccessful when closed cultures were used. only when gas exchange was permitted between culture atmosphere and ambient or when atmospheric volume was large did arabidopsis produce seeds. some factor(s) in the atmosphere limited seed production. co2 was examined because of its nutrient status and is amenable to analysis. plants were grown a ...198411539004
the influence of space flight factors on viability and mutability of plants.the experiments with air-dried crepis capillaris seeds aboard the soyuz 16 spaceship and the orbital stations salyut 5, 6, 7 have revealed an increase in the frequency of aberrant cells in seedlings grown from flight-exposed seeds during the flight (experiment) and after the flight on earth (flight control) as compared to the ground-based control. the increase in seedlings grown during the flight is more significant than in the flight control. during the flight arabidopsis thaliana developed fro ...198411539645
plant growth, development and embryogenesis during salyut-7 flight.the growth and geotropic movements of roots and hypocotyls of lettuce have been studied on board the salyut 7 station in a stationary position and on the centrifuge at 0.01, 0.1 and 1 g. on the centrifuge at 0.1 and 0.01 g as well as under weightlessness, the final length of hypocotyls was by 8-16% greater than in control plants on the centrifuge at 1 g. the length of roots, however, was reduced by 17% at 0.01 g and under weightlessness; at 0.1 g their growth is much the same as at 1 g. on the e ...198411539644
ultrastructure and movements of cell organelles in the root cap of agravitropic mutants and normal seedlings of arabidopsis thaliana.the root anatomy and ultrastructure of the agravitropic arobidopsis thaliana l. mutants dwf and aux-1 were compared with the gravitropic mutant aux-2 and the wild type (wt) in an attempt to find an explanation for the lack of response to gravity. no differences were found in the organization of the root cap. the central part of the cap (columella) contains 5 storeys of developing, functioning and degenerating statocytes. their ultrastructure is very similar in all four types of plant. particular ...198411541083
inhibition of dimethylnitrosamine-induced mutagenesis in arabidopsis thaliana by diethyldithiocarbamate and carbon monoxide.diethyldithiocarbamate and carbon monoxide markedly inhibited the frequency of embryonic and chlorophyll mutations induced by the metabolism-requiring mutagen dimethylnitrosamine in the higher plant arabidopsis thaliana. in contrast, the monoamine oxidase substrates, tryptamine, benzylamine and 2-phenylethylamine, had no such effect. the mutagenicity of a direct-acting mutagen, n-methyl-n-nitrosourea, was not altered by these inhibitors or substrates.19846318104
a study of the role of glutamate dehydrogenase in the nitrogen metabolism of arabidopsis thaliana.glutamate-dehydrogenase (gdh, ec 1.4.1.2) activity and isoenzyme patterns were investigated in arabidopsis thaliana plantlets, and parallel studies were carried out on glutamine synthetase (gs, ec 6.3.1.2). both nadh-gdh and nad-gdh activities increased during plant development whereas gs activity declined. leaves deprived of light showed a considerable enhancement of nadh-gdh activity. in roots, both gdh activities were induced by ammonia whereas in leaves nitrogen assimilation was less importa ...198524249451
photorespiration-induced reduction of ribulose bisphosphate carboxylase activation level.leaf photosynthesis and ribulose bisphosphate carboxylase activation level were inhibited in several mutants of the c(3) crucifer arabidopsis thaliana which possess lesions in the photorespiratory pathway. this inhibition occurred when leaves were illuminated under a photorespiratory atmosphere (50% o(2), 350 microliters per liter co(2), balance n(2)), but not in nonphotorespiratory conditions (2% o(2), 350 microliters per liter co(2), balance n(2)). inhibition of carboxylase activation level wa ...198516664149
embryo-lethal mutants of arabidopsis thaliana: analysis of mutants with a wide range of lethal phases.embryo-lethal mutants of arabidopsis thaliana were isolated by treating mature seeds with an aqueous solution of ethyl methanesulfonate (ems), screening the resulting m-1 plants for siliques containing 25% aborted seeds following self-pollination, and verifying the presence of induced mutations in subsequent generations. thirty-two recessive lethals with a mendelian pattern of inheritance were examined in detail. developmental arrest of mutant embryos ranged from the zygotic stage of embryogenes ...198524254011
analysis of photosynthetic antenna function in a mutant of arabidopsis thaliana (l.) lacking trans-hexadecenoic acid.several lines of evidence support the proposal that the unusual chloroplast-specific lipid acyl group delta3,trans-hexadecenoic acid (trans-c(16:1)) stimulates the formation or maintenance of the oligomeric form of the light-harvesting chlorophyll a/b complex (lhcp). to assess the functional significance of this apparent association we have analyzed lhcp structure and function in a mutant of arabidopsis thaliana (l.) which lacks trans-c(16:1) by electrophoretic analysis of the protein-chlorophyl ...198516664340
neighborhood predictors of plant performance.we developed models of inter-individual interference to predict the fecundity of individuals in populations of the annual plant species, arabidopsis thaliana. an individual plant is modeled as having a neighborhood which is a circular area of fixed radius with the plant at its center. other plants which share the circle with the focal plant are termed neighbors of the focal plant. we developed an index of neighborhood interference which is the independent variable in a non-linear regression mode ...198528311598
a mutant of arabidopsis lacking a chloroplast-specific lipid.in order to investigate the functional significance of membrane lipid unsaturation, we have isolated a series of mutants of arabidopsis thaliana which are deficient in particular membrane fatty acids. the first of these mutants completely lacks delta3-trans-hexadecenoate, an acyl group that until now has been thought to play an important role in the structure or function of thylakoid membranes in photosynthetic eukaryotes. the apparent absence of any marked physiological effect of the mutation i ...198517796728
a soluble chloroplast protein catalyzes ribulosebisphosphate carboxylase/oxygenase activation in vivo.ribulosebisphosphate carboxylase/oxygenase (ec 4.1.1.39) (rubisco) must be fully activated in order to catalyze the maximum rates of photosynthesis observed in plants. activation of the isolated enzyme occurs spontaneously, but conditions required to observe full activation are inconsistent with those known to occur in illuminated chloroplasts. genetic studies with a nutant of arabidopsis thaliana incapable of activating rubisco linked two chloroplast polypeptides to the activation process in vi ...198524443088
fluence-response curves and action spectra for promotion and inhibition of seed germination in wildtype and long-hypocotyl mutants of arabidopsis thaliana l.the fluence-response curves of wildtype and long-hypocotyl mutants of arabidopsis thaliana l. for induction and inhibition of seed germination, expressed as percentage germination on probit scale against logarithm of fluence, are very different in shape. the mutants show reduced photoinhibition of hypocotyl growth in white light compared with wildtype, suggesting they are either mutated in phytochrome, the blue/uv-absorbing photosystem or some other red-absorbing photosystem. calculations of the ...198524249267
nitrate reductase deficient cell lines from diploid cell cultures and lethal mutant m2 plants of arabidopsis thaliana.cell suspensions of diploid arabidopsis thaliana were screened for resistance to chlorate on a medium with ammonium nitrate as the nitrogen source, and after plating on filters to increase the plating efficiency. thirty-nine lines were selected, four of which were still resistant after two years of subculturing on non-selective medium. of the latter lines three were nitrate reductase deficient but exhibited some residual nitrate reductase activity; the fourth line showed a high level of enzyme a ...198524247470
blue and green light-induced phototropism in arabidopsis thaliana and lactuca sativa l. seedlings.exposure time-response curves for blue and green light-induced phototropic bending in hypocotyls of arabidopsis thaliana (l.) heynh. and lactuca sativa l. seedlings are presented. these seedlings show significant phototropic sensitivity up to 540 to 550 nanometers. since wave-lengths longer than 560 nanometers do not induce phototropic bending, it is suggested that the response to 510 to 550 nanometers light is mediated by the specific blue light photoreceptor of phototropism. we advise care in ...198516664021
alterations in growth, photosynthesis, and respiration in a starchless mutant of arabidopsis thaliana (l.) deficient in chloroplast phosphoglucomutase activity.a mutant of arabidopsis thaliana (l.) heynh. which lacks leaf starch was isolated by screening for plants which did not stain with iodine. the starchless phenotype, confirmed by quantitative enzymic analysis, is caused by a single recessive nuclear mutation which results in a deficiency of the chloroplast isozyme of phosphoglucomutase. when grown in a 12-h photoperiod, leaves of the wild-type accumulated substantial amounts of starch but lower levels of soluble sugars. under these conditions, th ...198516664354
arabidopsis thaliana and plant molecular genetics.arabidopsis thaliana is a small flowering plant with various properties that make it an excellent organism for experiments in molecular genetics. these properties include having a small nuclear genome, a near absence of dispersed repetitive dna, and a generation time of 4 to 5 weeks. in addition, mutations that affect hormone synthesis and response, many different enzyme activities, and numerous developmental processes have been identified and characterized.198517770799
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