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effects of amendments on the uptake and distribution of ddt in cucurbita pepo ssp pepo plants.the effects of soil amendments on the phytoextraction of summation operatorddt (ddt + ddd + dde) from soil ([ summation operatorddt] approximately 1500 ng/g) by a pumpkin variety of cucurbita pepo ssp pepo were tested and the patterns of summation operatorddt storage throughout the plant shoot were examined. the soil amendments did not increase the total amount of summation operatorddt extracted into plant shoots, but new information about summation operatorddt distribution in the plants was obt ...201019762136
kinetics of prolonged photoinhibition revisited: photoinhibited photosystem ii centres do not protect the active ones against loss of oxygen evolution.photoinhibition of photosystem ii (psii) in lincomycin-treated leaves begins as a first-order reaction, but fluorescence measurements have suggested that after prolonged illumination, the number of active psii centres stabilizes to 15-20% of control. the stabilization has been interpreted to indicate that photoinhibited psii centres protect the remaining active centres against photoinhibition (lee, hong and chow, planta 212:332-342, 2001). in an attempt to study the mechanism of this protection, ...201019760110
reversible change of extracellular ph at the generation of mechano-induced electrical reaction in a stem of cucurbita pepo.as was shown recently the generation of cooling-induced action potential accompanies the reversible change of extracellular ph.1 the ph changes are linked to the ca(2+)-induced transient inhibition of the plasma membrane h(+)-atpase. generation of mechano-induced local bioelectrical reaction (lber) also accompanies the reversible change of extracellular ph. shifts of ph medium during generation of mechano-induced electrical impulse are less than during generation of cooling-induced bioelectrical ...200719704676
risk assessment of genetically engineered crops: fitness effects of virus-resistance transgenes in wild cucurbita pepo.the development of crops genetically engineered for pathogen resistance has raised concerns that crop-to-wild gene flow could release wild or weedy relatives from regulation by the pathogens targeted by the transgenes that confer resistance. investigation of these risks has also raised questions about the impact of gene flow from conventional crops into wild plant populations. viruses in natural plant populations can play important roles in plant fecundity and competitive interactions. here, we ...200919688918
rapid diagnosis of plant virus diseases by transmission electron microscopy.a clear and rapid diagnosis of plant virus diseases is of great importance for agriculture and scientific experiments in plant phytopathology. even though negative staining and transmission electron microscopy (tem) are often used for detection and identification of viral particles and provide rapid and reliable results, it is necessary to examine ultrastructural changes induced by viruses for clear identification of the disease. with conventional sample preparation for tem it can take several d ...200919665483
the effects of planting methods and head pruning on seed yield and yield components of medicinal pumpkin (cucurbita pepo subsp. pepo convar. pepo var. styriaca) at low temperature areas.this experiment carried out to evaluate the effects of planting methods (seed sowing and transplanting) and head pruning (no pruning, pruning after 12th node and pruning after 16th node) on yield and yield components such as number of branches (sub-branches) per plant, fruits per plant, growth, fruit size, weight of fresh fruit, weight of seeds per fruit, number of seeds per fruit and seed yield of medicinal pumpkin. the experiment was carried out based of factorial experiment with randomized co ...200919580007
enhancing the release and plant uptake of pahs with a water-soluble purine alkaloid.the effect of a common plant alkaloid, caffeine, on the release and plant uptake of some polycyclic aromatic hydrocarbons (pahs) in soils was investigated. cucurbita pepo (ssp. pepo cv. gold rush) was grown in pah-spiked media in the presence and absence of caffeine. solubility tests initially confirmed the ability of caffeine to dissolve pahs mixtures of anthracene, phenanthrene, pyrene, benzo[a]pyrene and benzo[ghi]perylene. extraction experiments also highlighted its potential as a pah-releas ...200919477480
wild bee abundance and pollination service in cultivated pumpkins: farm management, nesting behavior and landscape effects.recent declines in managed and feral honey bee populations have greatly increased interest in the current and potential role of wild pollinators in agricultural pollination. pumpkin (cucurbita pepo l.) has great potential to be served by wild pollinators because of a reliable and widespread group of bee species that are commonly associated with their flowers, including bumble bees (bombus spp.), and, in the americas, two genera of specialist ground nesting bees (peponapis and xenoglossa). we exa ...200919449636
cadmium induced changes in subcellular glutathione contents within glandular trichomes of cucurbita pepo l.plants cope with cadmium (cd) stress by complexation with phytochelatins (pc), metallothioneins and glutathione and sequestration within vacuoles. especially glutathione was found to play a major role in cd detoxification as cd shows a high binding affinity towards thiols and as glutathione is a precursor for pc synthesis. in the present study, we have used an immunohistochemical approach combined with computer-supported transmission electron microscopy in order to measure changes in the subcell ...201019424775
spent mushroom substrates as component of growing media for germination and growth of horticultural plants.this research work was conducted in order to investigate the possibility of using spent mushroom substrate (sms) in the production of horticultural seedlings replacing part of the peat in the growing media. three vegetable species with different salt sensitivities, the less sensitive being tomato (lycopersicon esculentum var. muchamiel), the moderately salt-sensitive being courgette (cucurbita pepo l. var. afrodite f1) and the most salt-sensitive being pepper (capsicum annum l. var. lamuyo f1) w ...200919409775
influence of cover crop and intercrop systems on bemisia argentifolli (hemiptera: aleyrodidae) infestation and associated squash silverleaf disorder in zucchini.field experiments were conducted to evaluate the effects of cover cropping and intercropping on population densities of silverleaf whitefly, bemisia argentifolli bellow and perring, and the incidence of squash silverleaf disorder (ssl) in zucchini, cucurbita pepo l., in oahu, hi. two cover crops, buckwheat (bw), fagopyrum esculentum moench, and white clover (wc), trifolium repens l., or sunn hemp (sh), crotolaria juncea l., and an intercropped vegetable, okra, abelmonchus esculentus l., were eva ...200919389294
nanoparticle penetration and transport in living pumpkin plants: in situ subcellular identification.in recent years, the application of nanotechnology in several fields of bioscience and biomedicine has been studied. the use of nanoparticles for the targeted delivery of substances has been given special attention and is of particular interest in the treatment of plant diseases. in this work both the penetration and the movement of iron-carbon nanoparticles in plant cells have been analyzed in living plants of cucurbita pepo.200919389253
initial formation of an indigenous crop complex in eastern north america at 3800 b.p.although geneticists and archaeologists continue to make progress world-wide in documenting the time and place of the initial domestication of a growing number of plants and animals, far less is known regarding the critically important context of coalescence of various species into distinctive sets or complexes of domesticates in each of the world's 10 or more independent centers of agricultural origin. in this article, the initial emergence of a crop complex is described for one of the best-doc ...200919366669
inhibitory effect of watermelon mosaic virus on meloidogyne javanica (treub) chitwood infecting cucurbita pepo l. 198419295884
partitioning yield loss on yellow squash into nematode and insect components.the effect of a contplex of several insect and nematode pests on yield of yellow squash (cucurbita pepo l.) was examined in two field tests in southern florida. applications of permethrin for insect control and oxamyl primarily for nematode control plus some insect control were made alone and in combination to achieve differential reduction of various insect and nematode components contributing to yield loss. the effect of these components on yield was further analyzed by multiple regression. yi ...198219295683
management of root-knot nematodes by phenamiphos applied through an irrigation simulator with various amounts of water.phenamiphos (6.7 kg a.i./ha) was applied via an irrigation simulator to squash at planting (ap) and 2 weeks after planting (pp), and to corn ap and 1 week pp to manage root-knot nematodes (meloidogyne incognita). the nematicide was applied with 0.25, 0.64, 1.27, and 1.91 cm surface water/ ha to a lakeland sand in which the soil moisture was at or near field capacity. based on efficacy and crop response, no additional benefits resulted when phenamiphos was applied in volumes of water greater than ...198619294192
potential of crops uncommon to alabama for management of root-knot and soybean cyst nematodes.vigna unguiculata, cassia fasiculata, and sesamum indicum did not support meloidogyne arenaria, m. incognita, or heterodera glycines race 4 in greenhouse studies with soils from peanut and soybean fields. fagopyron eseulentum, cyamopsis tetragonoloba, and cucurbita pepo were hosts to the two meloidogyne spp. but were nonhosts to h. glycines. meloidogyne arenaria and m. incognita galled but reproduced poorly in the roots of three types of amaranthus cruentus, and low densities of these two meloid ...198819290317
effects of rapeseed and vetch as green manure crops and fallow on nematodes and soil-borne pathogens.in a rapeseed-squash cropping system, meloidogyne incognita race 1 and m. javanica did not enter, feed, or reproduce in roots of seven rapeseed cultivars. both nematode species reproduced at low levels on roots of the third crop of rapeseed. reproduction of m. incognita and m. javanica was high on squash following rapeseed, hairy vetch, and fallow. the application of fenamiphos suppressed (p = 0.05) root-gall indices on squash following rapeseed, hairy vetch, and fallow; and on dwarf essex and c ...199219283212
crop rotation studies with velvetbean (mucuna deeringiana) for the management of meloidogyne spp.results from a greenhouse experiment at cabrils, spain, with two velvetbean (mucuna deeringiana) accessions (florida and mozambique) growing in sterilized sandy loam and inoculated with meloidogyne arenaria race 2, m. incognita race 1, and m. javanica revealed that the legume was not a host for these nematodes. in contrast, roots of 'clemson spineless' okra (hibiscus esculentum), 'summer crookneck' squash (cucurbita pepo), and 'davis' soybean (glycine max) were galled by all three root-knot nema ...199219283043
efficacy of fenamiphos formulations applied through irrigation for control of meloidogyne incognita on squash.management ofmeloidogyne incognita by chemigation with fenamiphos was studied in an infested field planted to m. incognita-suscepfible yellow summer squash cv. dixie hybrid. fenamiphos (vl 73.1% a.i. manufacturing concentrate in propylene glycol) was mixed with unitol dsr-90 or used as fenamiphos 3 sc (spray concentrate). both formulations, applied with 63.5 kl irrigation water per hectare, decreased numbers of m. incognita second-stage juveniles in the soil and root-gall indices, and increased ...199419279950
tropical rotation crops influence nematode densities and vegetable yields.the effects of eight summer rotation crops on nematode densities and yields of subsequent spring vegetable crops were determined in field studies conducted in north florida from 1991 to 1993. the crop sequence was as follows: (i) rotation crops during summer 1991; (ii) cover crop of rye (secale cereale) during winter 1991-92; (iii) 'lemondrop l' squash (cucurbita pepo) during spring 1992; (iv) rotation crops during summer 1992; (v) rye during winter 1992-93; (vi) 'classic' eggplant (solanum melo ...199419279897
effects of tropical rotation crops on meloidogyne arenaria population densities and vegetable yields in microplots.the effects of 12 summer crop rotation treatments on population densities of meloidogyne arenaria race 1 and on yields of subsequent spring vegetable crops were determined in microplots. the crop sequence was: (i) rotation crops during summer 1991 ; (ii) cover crop of rye (secale cereale) during winter 1991-92; (iii) squash (cucurbita pepo) during spring 1992; (iv) rotation crops during summer 1992; (v) rye during winter 1992-93; (vi) eggplant (solanum melongena) during spring 1993. the 12 rotat ...199419279880
effects of mixed cropping on a soil nematode community in honduras.nematode-resistant tropical legumes are effective in reducing populations of plant-parasitic nematodes when used in rotation systems. mixed cropping is a common practice of many small farmers in central america, but little is known about the effects of tropical legumes on nematode communities under these systems. to examine the effects of intercropping on the nematode fauna associated with squash (cucurbita pepo) and cucumber (cucumis sativa) in honduras, two field experiments were conducted to ...199319279824
effects and carry-over benefits of nematicides in soil planted to a sweet corn-squash-vetch cropping system.the effects of irrigation on the efficacy of nematicides on meloidogyne incognita race 1 population densities, yield of sweet corn, and the carry-over of nematicidal effect in the squash crop were determined in a sweet corn-squash-vetch cropping system for 3 years. fenamiphos 15g and aldicarb 15g were applied at 6.7 kg a.i./ha and incorporated 15 cm deep with a tractor-mounted rototiller. ethylene dibromide (edb) was injected at 18 kg a.i./ha on each side of the sweet corn rows (total 36 kg a.i. ...199519277323
effect of simulated rainfall on leaching and efficacy of fenamiphos.there is increasing concern in the united states about the pesticide movement in soil, groundwater contamination, and pesticide residue in food. the objective of this study was to determine the efficacy, degradation, and movement of fenamiphos (nemacur 15g) in the soil and residues in squash fruit as influenced by four simulated rainfall treatments (2.5 or 5.0 cm each applied 1 or 3 days after nematicide application) under field conditions. in 1990, concentrations of fenamiphos were greater in t ...199519277322
effect of yard waste compost on plant-parasitic nematode densities in vegetable crops.the effects of yard-waste compost on densities of plant-parasitic nematodes were determined on four crops at two sites in north florida. separate experiments were conducted with sweet corn (zea mays), cowpea (vigna unguiculata), yellow squash (cucurbita pepo), and okra (hibiscus esculentus). in each test, the design was a randomized complete block replicated four times and involving three treatments: 269 mt/ha yard-waste compost applied to the soil surface as a mulch, 269 mt/ha compost incorpora ...199519277320
rotations with coastal bermudagrass and fallow for management of meloidogyne incognita and soilborne fungi on vegetable crops.the efficacy of fallow and coastal bermudagrass (cynodon dactylon) as a rotation crop for control of root-knot nematode (meloidogyne incognita race 1) and soilborne fungi in okra (hibiscus esculentus cv. emerald), squash (cucurbita pepo cv. dixie hybrid), and sweet corn (zea mays cv. merit) was evaluated in a 3-year field trial. numbers of m. incognita in the soil and root-gall indices were greater on okra and squash than sweet corn and declined over the years on vegetable crops following fallow ...199519277312
responses of meloidogyne arenaria and m. incognita to green manures and supplemental urea in glasshouse culture.the recent loss of many effective nematicides has led to renewed interest in alternative methods of nematode management. greenhouse experiments were conducted to determine the effects of rapeseed and velvetbean green manures, and supplemental urea, on the root-knot nematodes meloidogyne arenaria and m. incognita. green manures were incorporated with m. arenaria-infested soil using rates totaling 200,300, and 400 mg n/kg soil. squash plants grown in this soil were evaluated using a gall index and ...199619277190
effects of solarization and ammonium amendments on plant-parasitic nematodes.the effects of soil solarization and ammonium bicarbonate or ammonium sulfate against plant-parasitic nematodes on yellow squash (cucurbita pepo) and on vinca (catharanthus roseus) were evaluated at two sites. solarization for 3 weeks in the spring suppressed population levels of belonolaimus longicaudatus, criconemella spp., and dolichodorus heterocephalus throughout the growing season on both crops at both sites. levels of meloidogyne incognita were suppressed initially, but population densiti ...200019271007
rate response of 1,3-dichloropropene for nematode control in spring squash in deep sand soils.the soil fumigant 1,3-dichloropropene (1,3-d) formulated with chloropicrin is viewed as a likely alternative for replacing methyl bromide in florida when the latter is phased out in 2005. therefore, it behooves us to learn more about using 1,3-d in deep, sand soils. two trials were conducted on spring squash to determine the most effective rate of 1,3-d for the control of meloidogyne spp. rates tested included 0, 56, 84, 112, and 168 liters/ha of 1,3-d applied broadcast with conventional chisels ...200019271005
effects of a resistant corn hybrid and fenamiphos on meloidogyne incognita in a corn-squash rotation.the efficacy of a double-cross corn (zea mays) hybrid (old raccoon selection x t216) x (tebeau selection x mp 307) resistant to meloidogyne incognita as a rotational crop, and fenamiphos treatment for management of root-knot nematode (m. incognita race 1) in squash (cucurbita pepo var. melopepo) was evaluated in field tests during 1996 and 1997. numbers of m. incognita in the soil and root-gall indices were lower on the resistant hybrid than on a commercial cultivar dekalb dk-683. treatment mean ...199919270888
effect of crotalaria juncea amendment on squash infected with meloidogyne incognita.two greenhouse experiments were conducted to examine the effect of crotalaria juncea amendment on meloidogyne incognita population levels and growth of yellow squash (cucurbita pepo). in the first experiment, four soils with a long history of receiving yard waste compost (ywc+), no-yard-waste compost (ywc-), conventional tillage, or no-tillage treatments were used; in the second experiment, only one recently cultivated soil was used. half of the amount of each soil received air-dried residues of ...200419262819
effect of crotalaria juncea amendment on nematode communities in soil with different agricultural histories.effect of sunn hemp (crotalaria juncea) hay amendment on nematode community structure in the soil surrounding roots of yellow squash (cucurbita pepo) infected with root-knot nematodes was examined in two greenhouse experiments. soils were from field plots treated long-term (lt) with yard-waste compost or no yard-waste compost in lt experiment, and from a short-term (st) agricultural site in st experiment. soils collected were either amended or not amended with c. juncea hay. nematode communities ...200319262764
ultraweak and induced photon emission after wounding of plants.the ultraweak and induced photon emission were measured by a single photon counting equipment (photomultiplier hamamatsu r562) on cucurbita pepo variaca styriacae after wounding. wounding significantly changes the emission from a stationary to a nonstationary state and the shape of the decay curve obtained after light illumination. the rise in the ultraweak photon emission depends on the kind of wounding and its localization on the plant. the decay curves obtained after wounding could be better ...200919254235
effect of home freezing and italian style of cooking on antioxidant activity of edible vegetables.in this study, we analyzed the modifications of antioxidant activity consequent to 3 typical home cooking practices (steaming, boiling, and microwave cooking) in fresh and home frozen vegetables. six different vegetable species were examined: carrots (daucus carota l.), zucchini (cucurbita pepo l.), tomatoes (solanumn lycopersicum l.), green beans (phaseolus vulgaris l.), peas (pisum sativum l.), and yellow peppers (capsicum annuum l.). all vegetables were conventional products and were analyzed ...200819241586
monitoring diabrotica v. virgifera (col.: chrysomelidae) in southeastern slovenia: increasing population trend and host spectrum expansion.ever since the western corn rootworm (wcr) (diabrotica virgifera virgifera), an alien invasive species from north america, has been introduced into europe on at least 3 separate occasions, it spread within 15 years over the entire area of south-eastern and central europe (except denmark). until quite recently, zea mays l. was the only known host plant whereas in north america wcr also attacks members of the plant family cucurbitaceae. in august of 2006, we were able to validate these findings al ...200819226789
plant mitochondria electron partitioning is independent of short-term temperature changes.we tested the hypotheses that relative activity of the less efficient alternative oxidase (aox) path changes with diurnal temperature changes, and thus changes carbon use efficiency with temperature. the activities of the alternative and cytochrome oxidase (cox) paths in plant tissues of three species were determined by measuring 18o/16o discrimination and total respiration from 17 to 36 degrees c. a new, more accurate method for calculating oxygen uptake rate from the mass spectrometry data was ...200919210639
genes for "reverse" fruit striping in squash (cucurbita pepo).longitudinal striping is a common fruit color pattern in cucurbita pepo and is conferred by any of several alleles at the light coloration-1 locus. normally, dark stripes appear over the fruit surface areas between the 10 main subepidermal vein tracts with the lighter background color over and adjacent to these vein tracts. less commonly, the striping is "reversed," that is, lighter than the background color. the present work was conducted to elucidate the mode of inheritance of reverse striping ...200919153086
the effect of stem pruning and nitrogen levels of on some physico-chemical characteristics of pumpkin seed (cucurbita pepo l.).to investigate the effects of stem pruning (no heading, head pruning of stem after formation of 10 and 14 nodes) and nitrogen levels (0, 50, 100 and 200 kg ha(-1)) on physical and chemical characteristic of pumpkin seed a factorial experiment based on randomized complete block design with three replication was carried out in gorgan at 2003 and repeated in 2004 years. results showed that the stem pruning has significant effect on traits such as seed oil, linoleic acid and oleic acid content. nitr ...200719093491
population size and incidence of virus infection in free-living populations of cucurbita pepo.impact assessments of virus resistance transgene introgression into wild, free-living populations are important for determining whether these transgenes present a risk to agriculture or the environment. transgenic virus-resistant cucurbita pepo ssp. ovifera var. ovifera l. (squash) cultivars have been commercialized, and may be cultivated in close proximity to cross-compatible wild, free-living relatives (c. pepo subsp. pepo vars. ozarkana and texana). therefore, the potential impact of these vi ...200819081007
synergic effect studies of the bi-enzymatic system laccase-peroxidase in a voltammetric biosensor for catecholamines.several bi-enzymatic carbon paste biosensors modified with enzymes laccase from pleurotus ostreatus fungi and peroxidase from zucchini (cucurbita pepo) were constructed for evaluating the synergic effect of the two enzymes on the voltammetric biosensor response for various catecholamines. initially was investigated the effect of ph from 5.0 to 7.5, temperature from 25 to 50 degrees c, initial stirring time from 30 to 150 s, scan rate from 10 to 60 mvs(-1) and potential pulse amplitude from 10 to ...200318968977
ssr-based genetic linkage map of cucurbita moschata and its synteny with cucurbita pepo.the first ssr-based genetic linkage map of cucurbita moschata was created by integrating the maps of two f2 populations with one common parent developed from the crosses waltham butternut (wb) x nigerian local (nl) and zhou (a hull-less type) x wb. the integrated c. moschata map comprises 205 ssr markers and two morphological traits (gr and n). the map is composed of 27 linkage groups with a marker density of 7 cm. comparing the c. moschata map with the published cucurbita pepo map, we found a h ...200818956020
effect of ripe fruit pulp extract of cucurbita pepo linn. in aspirin induced gastric and duodenal ulcer in rats.a significant decrease in alkaline phosphatase (ap) activity and mucosal thickness and increase in ulcer index (ui) was observed in aspirin treated stomach and duodenum of albino rats. however, pretreatment with c. pepo fruit pulp extract for 14 consecutive days showed increase in ap activity and mucosal thickness along with decrease in ui, suggesting gastroduodenal protective and anti-ulcerogenic properties of c. pepo.200818949893
identification and characterization of a novel whitefly-transmitted member of the family potyviridae isolated from cucurbits in florida.abstract a novel whitefly-transmitted member of the family potyviridae was isolated from a squash plant (cucurbita pepo) with vein yellowing symptoms in florida. the virus, for which the name squash vein yellowing virus (sqvyv) is proposed, has flexuous rod-shaped particles of approximately 840 nm in length. the experimental host range was limited to species in the family cucurbitaceae, with the most dramatic symptoms observed in squash and watermelon, but excluded all tested species in the fami ...200718944369
melon resistance to cucurbit yellow stunting disorder virus is characterized by reduced virus accumulation.abstract the pattern of accumulation of cucurbit yellow stunting disorder virus (cysdv; genus crinivirus, family closteroviridae) rna has been analyzed in several cucurbit accessions. in susceptible accessions of melon (cucumis melo), cucumber (cucumis sativus), marrow (cucurbita maxima), and squash (cucurbita pepo), cysdv rna accumulation peaked during the first to second week postinoculation in the first to third leaf above the inoculated one; younger leaves showed very low or undetectable lev ...200318943165
differential uptake for dioxin-like compounds by zucchini subspecies.the zucchini (cucurbita pepo) cultivars 'patty green', 'black beauty', and 'gold rush' were cultivated on weathered dioxin-contaminated soil in pots, and concentrations of the 29 dioxin-like compounds that were assigned who-tefs, three non-toxic polychlorinated dibenzo-p-dioxins/dibenzofurans (pcdd/fs), and two non-dioxin-like polychlorinated biphenyls (pcbs) were analyzed. toxic equivalent (teq) values accumulated in 'black beauty' and 'gold rush' were about 180 times higher than those in 'patt ...200818835616
senescence progression in a single darkened cotyledon depends on the light status of the other cotyledon in cucurbita pepo (zucchini) seedlings: potential involvement of cytokinins and cytokinin oxidase/dehydrogenase activity.darkness mediates different senescence-related responses depending on the targeting of dark treatment (whole plants or individual leaves) and on the organs that perceive the signal (leaves or cotyledons). as no data are available on the potential role of darkness to promote senescence when applied to individual cotyledons, we have investigated how darkness affects the progression of senescence in either a single or both individually darkened cotyledons of young 10-day-old cucurbita pepo (zucchin ...200818823328
the effects of repeated planting, planting density, and specific transfer pathways on pcb uptake by cucurbita pepo grown in field conditions.an in situ field investigation into the potential of pcb phytoextraction by cucurbita pepo ssp. pepo (pumpkin) plants was continued for a second year at a field site known to be contaminated with a mixture of aroclors 1254 and 1260 (average soil [pcb]=21 microg/g). plant stem and leaf pcb concentrations in this second field season (11 and 8.9 microg/g, respectively) were observed to increase significantly from the stem and leaf pcb concentrations reported in the previous year (5.7 and 3.9 microg ...200818786697
an easy method using glutaraldehyde-introduced fluorescence for the microscopic analysis of plant biotrophic interactions.the method introduced in this article makes use of the glutaraldehyde-induced auto-fluorescence of proteins after cross-linking with glutaraldehyde for the analysis of cellular and sub-cellular structures. because the interface of biotrophic interactions is rich in proteins, the method presented is particularly suitable for the analysis of such interactions; we have exemplified its usefulness by analyzing (1) the root feeding sites induced in roots from arabidopsis thaliana by the root-knot nema ...200818778431
papaya ringspot virus-p: characteristics, pathogenicity, sequence variability and control.taxonomy: papaya ringspot virus (prsv) is an aphid-transmitted plant virus belonging to the genus potyvirus, family potyviridae, with a positive sense rna genome. prsv isolates belong to either one of two major strains, p or w. the p strains infect both papaya and cucurbits whereas the w strains infect only cucurbits. geographical distribution: prsv-p is found in all major papaya-growing areas. physical properties: virions are filamentous, non-enveloped and flexuous measuring 760-800 x 12 nm. vi ...200818705869
influence of compost amendment on pyrene availability from artificially spiked soil to two subspecies of cucurbita pepo.the dissolved organic matter (dom) fraction of soil organic matter (som) may positively contribute to polycyclic aromatic hydrocarbons (pahs) bioavailability. this work investigated the effects of dom-rich and pahs-free compost amendment on the plant uptake of pyrene. two subspecies of cucurbita pepo (ssp. pepo cv. raven and ssp. texana cv. sunray) were grown for three weeks in a spiked soil containing 83.9 mg kg(-1) pyrene under four different treatments; inorganic fertilizer (if) alone, 15% (v ...200818632137
pancreatic proteolytic enzymes of ostrich purified on immobilized protein inhibitors. characterization of a new form of chymotrypsin (chtr1).four forms of chymotrypsin (chtr1, chtr2, chtr3, chtr4), one form of trypsin and one form of elastase were purified from a slightly alkaline extract of ostrich (struthio camelus) pancreas. the zymogens in the crude extract were activated with immobilized trypsin and then separated by affinity chromatography using immobilized inhibitors and ion exchange chromatography. one of the purified forms of chymotrypsin (chtr1) exhibited an unusual interaction with the highly selective protein trypsin inhi ...200818598777
differential effects of methyl jasmonate on growth and division of etiolated zucchini cotyledons.the jasmonates are well studied in the context of plant defence but increasingly are also recognised as playing roles in development. in many systems, jasmonates antagonise the effects of cytokinins. the aim of the present work was to elucidate interactions between methyl jasmonate and cytokinin (benzyladenine) in regulating growth of zucchini (cucurbita pepo l., cv. cocozelle, var. tripolis) cotyledons, taking advantage of the ability to simultaneously quantify cell enlargement and division fro ...200818557907
response of citrus peelminer marmara gulosa guillén and davis (lepidoptera: gracillariidae) stages to various insecticides.egg and larval stages of citrus peelminer marmara gulosa guillén and davis infesting zucchini squash cucurbita pepo l. var 'revenue' were exposed in the laboratory to a wide range of insecticide classes used in california citrus (organophosphate, carbamate, pyrethroid, neonicotinoid, insect growth regulator, fermentation products and sulfur) to determine peelminer response with and without a non-ionic adjuvant or oil.200818506674
ascorbic acid conjugates isolated from the phloem of cucurbitaceae.analysis of phloem exudates from the fruit of cucurbitaceae revealed the presence of several compounds with uv-visible absorption spectra identical to that of l-ascorbic acid. in cucurbita pepo l. (zucchini), the compounds could be isolated from phloem exudates collected from aerial parts of the plant but were not detected in whole tissue homogenates. the compounds isolated from the phloem exudates of c. pepo fruit were eluted from strong anion exchange resin in the same fraction as l-ascorbic a ...200818472116
sunflower (helianthus annuus l.) as a pre-columbian domesticate in mexico.mexico has long been recognized as one of the world's cradles of domestication with evidence for squash (cucurbita pepo) cultivation appearing as early as 8,000 cal b.c. followed by many other plants, such as maize (zea mays), peppers (capsicum annuum), common beans (phaseolus vulgaris), and cotton (gossypium hirsutum). we present archaeological, linguistic, ethnographic, and ethnohistoric data demonstrating that sunflower (helianthus annuus) had entered the repertoire of mexican domesticates by ...200818443289
diabrotica virgifera virgifera leconte: inconspicuous leaf beetle--formidable challenges to agriculture.in the universe of entomology with its close to one million described and an estimated ten million undescribed species, diabrotica virgifera virgifera leconte (coleoptera: chrysometidae), the western corn rootworm (wcr), is one of the economically most important pests. it causes annual treatment costs and damages of a billion dollars in the us (metcalf 1986). similar costs are predicted for europe which the beetle invaded 15 years ago. due to lack of natural enemies it is now expanding its terri ...200718399421
microsatellites for the genus cucurbita and an ssr-based genetic linkage map of cucurbita pepo l.until recently, only a few microsatellites have been available for cucurbita, thus their development is highly desirable. the austrian oil-pumpkin variety gleisdorfer olkürbis (c. pepo subsp. pepo) and the c. moschata cultivar soler (puerto rico) were used for ssr development. ssr-enriched partial genomic libraries were established and 2,400 clones were sequenced. of these 1,058 (44%) contained an ssr at least four repeats long. primers were designed for 532 ssrs; 500 primer pairs produced fragm ...200818379753
purine-containing cucurbitane triterpenoids from cucurbita pepo cv dayangua.phytochemical investigation of the fruits of cucurbita pepo cv dayangua led to the isolation of cucurbitaglycosides a (1) and b (2). this is the first report of cucurbitane triterpenoids with a purine unit. their structures were elucidated mainly based on interpretation of hresims results, as well as 1d and 2d nmr spectra. cucurbitaglycosides a and b showed cytotoxic activity against the human epithelial carcinoma cell line hela with ic50 of 17.2 and 28.4 microg/ml, respectively.200818325551
impact of daily consumption of iron fortified ready-to-eat cereal and pumpkin seed kernels (cucurbita pepo) on serum iron in adult women.iron deficiency, anemia, is the most prevalent nutritional problem in the world today. the objective of this study was to consider the effectiveness of consumption of iron fortified ready-to-eat cereal and pumpkin seed kernels as two sources of dietary iron on status of iron nutrition and response of hematological characteristics of women at reproductive ages. eight healthy female, single or non pregnant subjects, aged 20-37 y consumed 30 g of iron fortified ready-to-eat cereal (providing 7.1 mg ...200718198398
dde remediation and degradation.ddt and its metabolites, ddd and dde, have been shown to be recalcitrant to degradation. the parent compound, ddt, was used extensively worldwide starting in 1939 and was banned in the united states in 1973. the daughter compound, dde, may result from aerobic degradation, abiotic dehydrochlorination, or photochemical decomposition. dde has also occurred as a contaminant in commercial-grade ddt. the p,p'-dde isomer is more biologically active than the o,p-dde, with a reported half-life of -5.7 ye ...200818069646
cold tolerance and plant growth promotion potential of serratia marcescens strain srm (mtcc 8708) isolated from flowers of summer squash (cucurbita pepo).to determine the cold tolerance and plant growth promotion potential of serratia marcescens strain srm (mtcc 8708).200818028329
uptake and translocation of p,p'-dichlorodiphenyldichloroethylene supplied in hydroponics solution to cucurbita.field studies show shoots of zucchini (cucurbita pepo l.) accumulate various hydrophobic contaminants from soil, although many other plants do not, including cucumber (cucumis sativus l.). to investigate the mechanism for this uptake, we presented p,p'-dichlorodiphenyldichloroethylene (dde) to these two species in hydroponics solution. a mixture of dde bound to tenax beads stirred with a solution of water passing through a reservoir provided a flowing solution containing dde at approximately 2 m ...200718020671
nanoparticles as smart treatment-delivery systems in plants: assessment of different techniques of microscopy for their visualization in plant tissues.the great potential of using nanodevices as delivery systems to specific targets in living organisms was first explored for medical uses. in plants, the same principles can be applied for a broad range of uses, in particular to tackle infections. nanoparticles tagged to agrochemicals or other substances could reduce the damage to other plant tissues and the amount of chemicals released into the environment. to explore the benefits of applying nanotechnology to agriculture, the first stage is to ...200817998213
cucurbitane and hexanorcucurbitane glycosides from the fruits of cucurbita pepo cv dayangua.phytochemical investigation of the fruits of cucurbita pepo cv dayangua has led to the isolation of two cucurbitane glycosides: cucurbitacin l 2-o-beta-d-glucopyranoside (1), cucurbitacin k 2-o-beta-d-glucopyranoside (2) and two hexanorcucurbitane glycosides: 2,16-dihydroxy-22,23,24,25,26,27-hexanorcucurbit-5-en-11,20-dione 2-o-beta-d-glucopyranoside (3) and 16-hydroxy-22,23,24,25,26,27-hexanorcucurbit-5-en-11,20-dione 3-o-alpha-l-rhamnopyranosyl-(1 --> 2)-beta-d-glucopyranoside (4). compounds 1 ...200917885839
content of nutrient and antinutrient in edible flowers of wild plants in mexico.nutrient and antinutritional/toxic factors present in some edible flowers consumed in mexico were determined. the edible flowers were: agave salmiana, aloe vera, arbutus xalapensis, cucurbita pepo (cultivated), erythrina americana, erythrina caribaea, euphorbia radians benth and yucca filifera. the nutrient content in the flowers studied is similar to that of the edible leaves and flowers studied mainly in africa. the moisture content of the flowers varied from 860 to 932 g kg(-1). crude protein ...200717768684
root hairs, cuticle, and pits.the filamentous roots of mustard (raphanus sativus), radish (brassica nigra), squash (cucurbita pepo), and wheat (triticum aestivum) are covered throughout their length with living nucleated root hairs which may measure 1600 micro or more. the outer walls of piliferous and nonpiliferous cells consist of successive layers of mucilage, cutin, and the cellulose-pectic framework of the cell. plasmodesmata and pits occur on all cell walls. under the electron microscope individual pores and pits in th ...196317746006
ethnoveterinary medicines used to treat endoparasites and stomach problems in pigs and pets in british columbia, canada.this paper documents the medicinal plants used to treat endoparasites and stomach problems in dogs, cats and pigs in british columbia, canada. ethnoveterinary data was collected over a 6-month period in 2003. the majority of the information on pets came from 2 naturopaths, 10 herbalists, 5 dog trainers, breeders and pet shop owners, 9 holistic veterinarians and 6 of 27 organic farmers. two pig farmers joined the study in the final stages. the following plants were used as anthelmintics: artemisi ...200717628343
[relationships between host preference of liriomyza huidobrensis (blanchard) and nutrient and chlorophyll contents in host foliage].the laboratory study with no free choice means showed that the host preference of liriomyza huidobrensis (blanchard) adult differed significantly with test plants. phaseolus vulgaris, chrysanthemum coronarium and cucurbita pepo were the most preferred, while lycopersicum esculenturn and brassica oleracea were the least. correlative analysis indicated that the host preference of l. huidobrensis adult had a significant positive correlation with the content of soluble sugar, but less correlation wi ...200717552217
surfactants differentially impact p,p'-dde accumulation by plant and earthworm species.the effect of four surfactants (triton x-100, tween-80, rhamnolipids, cyclodextrin) at 100-1000 mg/l on p,p'-dde phytoextraction by cucurbita pepo (zucchini) under field conditions and p,p'-dde bioaccumulation by earthworm species (eisenia fetida, lumbricus terrestris) under laboratory conditions was investigated. abiotically, surfactants (except cyclodextrin) increased contaminant desorption from soil by 4-fold, with higher concentrations generally promoting greater release. cyclodextrin had no ...200717533859
physiological and metabolic changes of cucurbita pepo leaves in response to zucchini yellow mosaic virus (zymv) infection and salicylic acid treatments.the changes of some physiological and biochemical parameters in pumpkin (cucurbita pepo cv eskandarani) leaves associated with zucchini yellow mosaic virus (zymv) infection and the effect of exogenous application of salicylic acid (sa) were studied in this paper. in comparison to the untreated leaves, zymv infected leaves showed many symptoms, including severe mosaic, size reduction, stunting and deformation. results from analysis of physiological parameters indicated that viral infection and sa ...200717466528
qualitative and quantitative differences in carotenoid composition among cucurbita moschata, cucurbita maxima, and cucurbita pepo.squashes and pumpkins are important dietary sources of carotenoids worldwide. the carotenoid composition has been determined, but reported data have been highly variable, both qualitatively and quantitatively. in the present work, the carotenoid composition of squashes and pumpkins currently marketed in campinas, brazil, were determined by hplc-dad, complemented by hplc-ms for identification. cucurbita moschata 'menina brasileira' and c. moschata 'goianinha' had similar profiles, with beta-carot ...200717444652
detection by rapd-pcr of polymorphism in populations of bemisia tabaci (genn.) collected on four host plants from iran.the sweetpotato whitefly, bernisia tabaci (genn.) (hom: aleyrodidae) is a major pest of field crops, vegetables and ornamentals in iran. in this study, the infested leaves of cucumber (cucurnis sativus l.) zucchini (cucurbita pepo l.) eggplant (solanum melongena l.) and cotton (gossypium hirsutum l.) with whitefly nymphs and pupae were collected from iran, and were transferred to the laboratory. the newly emerged males and females of each population were released separately into a large cage set ...200617385530
chlorophyllase is a rate-limiting enzyme in chlorophyll catabolism and is posttranslationally regulated.chlorophyll is a central player in harvesting light energy for photosynthesis, yet the rate-limiting steps of chlorophyll catabolism and the regulation of the catabolic enzymes remain unresolved. to study the role and regulation of chlorophyllase (chlase), the first enzyme of the chlorophyll catabolic pathway, we expressed precursor and mature versions of citrus (citrus sinensis) chlase in two heterologous plant systems: (1) squash (cucurbita pepo) plants using a viral vector expression system; ...200717369368
ethnomedicines used in trinidad and tobago for reproductive problems.throughout history women have tried to control or enhance their fertility using herbal remedies, with various levels of societal support. caribbean folk medicine has been influenced by european folk medicine, either through the early spanish and french settlers or through the continuous immigration of spanish-speaking peoples from venezuela. some folk uses are ancient and were documented by galen and pliny the elder.200717362507
characterization of esterases from cucurbita pepo cv. "eskandrani".two of the six esterases identified in cucurbita pepo cv. "eskandrani" were purified to homogeneity using two chromatography steps: anion exchange and gel filtration. the molecular weights of c. pepo esterases eic and eii were 50,000 +/- 1500 and 68,000 +/- 1900 da from gel filtration and 47,000 and 66,000 da from sds/page, respectively, suggesting a monomeric structure for both enzymes. esterases eic and eii had k(m) values of 1.22 and 1.56 mm and ph optima at 9.0 and 8.0, respectively. the sub ...200817321740
in vitro antioxidant properties of cucurbita pepo l. male and female flowers extracts.total antioxidant capacities, 2,2-diphenyl-1-picrylhydrazyl (dpph.), hydroxyl (ho.), scavenging activities, and total phenolic values were determined in extracts of cucurbita pepo l. female and male flowers. powdered c. pepo l. samples were extracted in aqueous ethyl acetate (ea: w1, 17:3), ethanol (e), and water (w) by agitating in magnetic stirrer for 80 degrees c, 15 min and also by in aqueous ethyl acetate (ea: w2, 17:3) at 25 degrees c, 15 min. dpph., ho. scavenging capacities and total phe ...200717308962
membrane associated qualitative differences in cell ultrastructure of chemically and high pressure cryofixed plant cells.membrane contrast can sometimes be poor in biological samples after high pressure freezing (hpf) and freeze substitution (fs). the addition of water to the fs-medium has been shown to improve membrane contrast in animal tissue and yeast. in the present study we tested the effects of 1% and 5% water added to the fs-medium (2% osmium with 0.2% uranyl acetate in anhydrous acetone) on the quality and visibility of membranes in high pressure frozen leaf samples of cucurbita pepo l. plants and compare ...200717270463
in situ phytoextraction of polychlorinated biphenyl - (pcb)contaminated soil.a pilot-scale field trial of phytoextraction of pcbs provides insight into the practical application of this technology, using the plant species cucurbita pepo ssp pepo cv. howden (pumpkin), carex normalis (sedge), and festuca arundinacea (tall fescue). this in situ trial took place at a historically contaminated field site, in soil contaminated with a mean concentration of 46 microg/g (range of 0.6 - 200 microg/g) total pcbs (aroclor 1254/1260). shoot bioaccumulation factors (where baf(shoot)=[ ...200717258285
differences in p,p'-dde bioaccumulation from compost and soil by the plants cucurbita pepo and cucurbita maxima and the earthworms eisenia fetida and lumbricus terrestris.two plant species, cucurbita pepo and cucurbita maxima, and two earthworm species, eisenia fetida and lumbricus terrestris, were exposed to soil and compost with equivalent p,p'-dde contamination. pollutant bioconcentration was equal in plant roots in both media, but translocation was higher in c. pepo. bioaccumulation by e. fetida was approximately 6- and 3-fold higher than that by l. terrestris in the soil and compost, respectively. for all species, p,p'-dde uptake was significantly greater fr ...200717241722
comparing contributions of soil versus root colonization to variations in stomatal behavior and soil drying in mycorrhizal sorghum bicolor and cucurbita pepo.in prior studies we learned that colonization of soil can be as important as colonization of roots in determining mycorrhizal influence on the water relations of host plants. here we use a path analysis modeling approach to test (a) whether quantity of hyphae in soil contributes to variations in stomatal behavior and soil drying, and (b) whether soil colonization or root colonization has a stronger influence on these stomatal and soil drying responses. experiments were performed on sorghum bicol ...200717189660
reactions, characterization and uptake of ammoxidized kraft lignin labeled with 15n.ammoxidation of kraft lignin was carried out in a parr reactor using (15)nh(3) as the main nitrogen source. reaction parameters were set up until a total nitrogen content of approximately 13 wt.% in lignin was achieved, in accordance with conditions of previous studies. analytical tools such as ftir, py-gc/ms, and solid state nmr were used in this research. the nature of nitrogen bondings is discussed. the incorporation of the (15)n from ammoxidized lignin was followed in pumpkins (zucchini cucu ...200717161600
virus-induced changes in the subcellular distribution of glutathione precursors in cucurbita pepo (l.).changes in glutathione contents occur in plants during environmental stress situations, such as pathogen attack, as the formation of reactive oxygen species leads to the activation of the antioxidative defence system. as glutathione is synthesized out of its constituents cysteine, glycine, and glutamate the availability of these components will limit glutathione synthesis in plants especially during stress situations and therefore the ability of the plant to fight oxidative stress. to gain a dee ...200717143806
artificial elevation of glutathione affects symptom development in zymv-infected cucurbita pepo l. plants.styrian oil pumpkin seedlings (cucurbita pepo l. subsp. pepo var. styriaca greb: .) were treated for 48 h with 1 mm otc (l-2-oxothiazolidine-4-carboxylic acid) in order to artificially increase cellular glutathione content. they were inoculated with zucchini yellow mosaic virus (zymv) 10 days later. the effects of otc treatment and zymv infection on glutathione levels were examined at the subcellular level by immunogold labeling of glutathione using a transmission electron microscope (tem). thes ...200717143780
isotopic enrichment in a phloem-feeding insect: influences of nutrient and water availability.the isotopic enrichment between an animal and its diet can vary among and within living systems, but the sources of variation are not yet fully understood. some studies have found that diet quality or an animal's nutritional status can influence the degree of trophic enrichment, while others have dismissed nutrition as a contributing factor. we evaluated the effects of nutrient and water availability on carbon and nitrogen isotopic enrichment in a specialized plant-herbivore system. aphids are l ...200717124569
growth conditions impact 2,2-bis(p-chlorophenyl)-1,1-dichloroethylene (p,p'-dde) accumulation by cucurbita pepo.laboratory experiments were conducted to study the effects of soil moisture content, planting density, plant age, and the growth of multiple generations on the bioconcentration of weathered p,p'-dde by the plant cucurbita pepo. as soil moisture content increased from 7.4% to 29.9% (by weight), rates of contaminant accumulation by plant roots were increased by more than a factor of 2. higher planting density also led to higher uptake, as the root bioconcentration factor (bcf, dry-weight ratio of ...200617120529
potential for phytoremediation of polychlorinated biphenyl-(pcb-)contaminated soil.weathered soils contaminated with commercial-grade aroclor 1260 from three sites in canada were used to investigate the polychlorinated biphenyl (pcb) phytoextraction potential of nine plant species (festuca arundinacea, glycine max, medicago sativa, phalaris arundinacea, lolium multiflorum, carex normalis, and three varieties of cucurbita pepo ssp. pepo) under controlled greenhouse conditions. the soils used varied in pcb concentration (90-4200 microg/g) and total organic content (0.06-2.02%). ...200617120525
ultrasonic treatment stimulates multiple shoot regeneration and explant enlargement in recalcitrant squash cotyledon explants in vitro.ultrasonic treatment (0.5-2 min) stimulated multiple shoot regeneration to high levels in vitro from recalcitrant cotyledon explants of commercial squash (cucurbita pepo l.) cultivars ma'yan and bareqet, on murashige and skoog [physiol plant 15:473-497, 1962] (regeneration) medium augmented with 4.4 microm benzyladenine. at this stage, unsonicated control explants regenerated only a few very small shoots or bud-like structures. ultrasound also stimulated massive explant growth. ultrasound treatm ...200717021848
fatty acids by high-performance liquid chromatography and evaporative light-scattering detector.a high-performance liquid chromatographic (hplc) separation method with an evaporative light-scattering detector (elsd) has been developed for the separation and quantitative analysis of fatty acid methyl esters (fame) in three different oils. reverse-phased c18 hplc separation of 13 fame is achieved using a methanol/water eluent mixture. the retention times (rt) reflect the elution behavior of these compounds on c18 reversed-phase hplc. the proposed method is tested on: soybean oil (glycine max ...200617007865
methyl jasmonate is a more effective senescence-promoting factor in cucurbita pepo (zucchini) cotyledons when compared with darkness at the early stage of senescence.the effects of short-term darkening and methyl jasmonate (meja) on cotyledon senescence were studied 24h after transfer of intact 7-day-old cucurbita pepo (zucchini) seedlings to darkness or spraying with 100 microm meja. the jasmonate inhibitory effect on chlorophyll content and chloroplast transcriptional activity was stronger compared with darkness. further, meja reduced the photosynthetic rate whereas darkness did not affect photosynthesis. neither stress factor affected the photochemical qu ...200716987568
antioxidative effects of pumpkin seed (cucurbita pepo) protein isolate in ccl4-induced liver injury in low-protein fed rats.the effects of pumpkin seed (cucurbita pepo) protein isolate on the plasma activity levels of catalase (ca), superoxide dismutase (sod), glutathione peroxidase (gshpx) and total antioxidant capacity (tac) as well as glucose-6-phosphatase (g6pase) in liver homogenates and lipid peroxidation (lpo-malondialdehyde-mda) levels in liver homogenates and liver microsomal fractions against carbon tetrachloride (ccl(4))-induced acute liver injury in low-protein fed sprague-dawley rats (rattus norvegicus) ...200616909447
phytoextraction of excess soil phosphorus.in the search for a suitable plant to be used in p phytoremediation, several species belonging to legume, vegetable and herb crops were grown in p-enriched soils, and screened for p accumulation potentials. a large variation in p concentrations of different plant species was observed. some vegetable species such as cucumber (cucumis sativus) and yellow squash (cucurbita pepo var. melopepo) were identified as potential p accumulators with >1% (dry weight) p in their shoots. these plants also disp ...200716904249
eastern north america as an independent center of plant domestication.the status of eastern north america as an independent center of plant domestication has recently been called into question by a number of genetic and archaeological studies, which suggest that the region may not have witnessed the independent domestication of local crop plants, but rather may have been on the receiving end of domesticated crop plants introduced from mexico. here, i provide a synthesis of the currently available archaeological and genetic evidence from both eastern north america ...200616894156
nectar resorption and translocation in cucurbita pepo l. and platanthera chlorantha custer (rchb.).nectar resorption and sugar translocation were studied in cucurbita pepo (cucurbitaceae) and platanthera chlorantha (orchidaceae) by micro-autoradiography. in both species, nectar was resorbed in pollinated and unpollinated flowers and ovules developing into seeds were found to be the main sugar sink. in c. pepo, the mobility of resorbed sugars in pollinated female flowers was higher than in unpollinated ones; male flowers showed lower mobility of resorbed sugar. in p. chlorantha, radioactivity ...200716883483
immunocytochemical localization of glutathione precursors in plant cells.in plant cells, the antioxidant glutathione is synthesized out of its constituents cysteine, glutamate and glycine. as information about their subcellular and cellular distribution was not available in the past, the aim of the present study was to develop a technique that would allow visualization of their distribution in plant cells. with selective antibodies against cysteine, glutamate and glycine, it was possible, for the first time, to study the relative distribution of these components in m ...200616861702
compensatory capsid protein mutations in cucumber mosaic virus confer systemic infectivity in squash (cucurbita pepo).cucumber mosaic virus (cmv) systemically infects both tobacco and zucchini squash. cmv capsid protein loop mutants with single-amino-acid substitutions are unable to systemically infect squash, but they revert to a wild-type phenotype in the presence of an additional, specific single-site substitution. the d118a, t120a, d192a, and d197a loop mutants reverted to a wild-type phenotype but did so in combination with p56s, p77l, a162v, and i53f or t124i mutations, respectively. the possible effect o ...200616840352
inhibition of testosterone-induced hyperplasia of the prostate of sprague-dawley rats by pumpkin seed oil.the oil from the pumpkin (cucurbita pepo) seed is claimed to be useful in the management of benign prostatic hyperplasia. this investigation seeks to examine the effect of pumpkin seed oil on testosterone-induced hyperplasia of the prostate of rats. hyperplasia was induced by subcutaneous administration of testosterone (0.3 mg/100 g of body weight) for 20 days. simultaneous oral administration of either pumpkin seed oil (2.0 and 4.0 mg/100 g of body weight) or corn oil (vehicle) was also given f ...200616822218
in vitro antioxidative activity of pumpkin seed (cucurbita pepo) protein isolate and its in vivo effect on alanine transaminase and aspartate transaminase in acetaminophen-induced liver injury in low protein fed rats.the antioxidative effects of pumpkin seed protein isolate (cucurbita pepo) were investigated in vitro. the isolate exhibited about 80% radical scavenging activity, chelating activity of approximately 64% on fe2+ ions and an inhibition of approximately 10% of xanthine oxidase. subsequently the effects of the isolate on the plasma activity levels of alanine transaminase and aspartate transaminase against acetaminophen induced acute liver injury in low-protein fed male sprague-dawley rats were asce ...200616807884
extracts from pygeum africanum and other ethnobotanical species with antiandrogenic activity.extracts from pygeum africanum, serenoa repens and cucurbita pepo are used in the treatment of benign prostatic hyperplasia (bph) and prostate cancer (pca). the activity of the androgen receptor (ar) is known to control growth of the prostate. here, we examined extracts of these plants for their antiandrogenic activity using an ar responsive reporter gene assay for drug discovery. a selective dichloromethane extract from the stem barks of pygeum africanum revealed the highest antiandrogenic effe ...200616783690
effect of mycorrhizal fungi on the phytoextraction of weathered p,p-dde by cucurbita pepo.field experiments were conducted to assess the impact of inoculation with mycorrhizal fungi on the accumulation of weathered p,p'-dde from soil by three cultivars of zucchini (cucurbita pepo spp. pepo cv costata romanesco, goldrush, raven). three commercially available mycorrhizal products (biovam, myco-vam, invam) were inoculated into the root system of the zucchini seedlings at planting. in agreement with our previous findings, plants not inoculated with fungi accumulated large but variable am ...200616777321
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