Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| kinetic and thermodynamic investigation on ascorbate oxidase activity and stability of a cucurbita maxima extract. | the kinetic and thermodynamic properties of ascorbate oxidase (ao) activity and stability of a cucurbita maxima extract were investigated. activity tests performed at 25 degrees c using initial ascorbic acid concentration in the range 50-750 m allowed estimating the michaelis constant for this substrate (km = 126 microm) and the maximum initial rate of ascorbic acid oxidation (a0,max = 1.57 mm min-1). the main thermodynamic parameters of the enzyme reaction (deltah* = 10.3 kj mol-1; deltag* = 87 ... | 2006 | 17137312 |
| the squash aspartic proteinase inhibitor sqapi is widely present in the cucurbitales, comprises a small multigene family, and is a member of the phytocystatin family. | the squash (cucurbita maxima) phloem exudate-expressed aspartic proteinase inhibitor (sqapi) is a novel aspartic acid proteinase inhibitor, constituting a fifth family of aspartic proteinase inhibitors. however, a comparison of the sqapi sequence to the phytocystatin (a cysteine proteinase inhibitor) family sequences showed approximately 30% identity. modeling sqapi onto the structure of oryzacystatin gave an excellent fit; regions identified as proteinase binding loops in cystatin coincided wit ... | 2006 | 17103059 |
| differences in p,p'-dde bioaccumulation from compost and soil by the plants cucurbita pepo and cucurbita maxima and the earthworms eisenia fetida and lumbricus terrestris. | two plant species, cucurbita pepo and cucurbita maxima, and two earthworm species, eisenia fetida and lumbricus terrestris, were exposed to soil and compost with equivalent p,p'-dde contamination. pollutant bioconcentration was equal in plant roots in both media, but translocation was higher in c. pepo. bioaccumulation by e. fetida was approximately 6- and 3-fold higher than that by l. terrestris in the soil and compost, respectively. for all species, p,p'-dde uptake was significantly greater fr ... | 2007 | 17241722 |
| qualitative and quantitative differences in carotenoid composition among cucurbita moschata, cucurbita maxima, and cucurbita pepo. | squashes and pumpkins are important dietary sources of carotenoids worldwide. the carotenoid composition has been determined, but reported data have been highly variable, both qualitatively and quantitatively. in the present work, the carotenoid composition of squashes and pumpkins currently marketed in campinas, brazil, were determined by hplc-dad, complemented by hplc-ms for identification. cucurbita moschata 'menina brasileira' and c. moschata 'goianinha' had similar profiles, with beta-carot ... | 2007 | 17444652 |
| monitoring protein cleavage and concurrent disulfide bond assignment using thermospray lc/ms. | the arrangement of the disulfide bridges of cucurbita maxima trypsin inhibitor, cmti i, has been confirmed by enzymatic and chemical cleavages of the native protein and analysis of the resulting disulfide-bridged fragments using thermospray liquid chromatography/mass spectrometry. although the disulfide bridges of cmti i have recently been assigned from the x-ray crystallographic structure, direct chemical analysis of the s-s bonds using classical techniques proved difficult. the cmti i molecule ... | 2007 | 2134058 |
| [effects of nacl stress on polyamine contents in grafted cucumber leaves]. | with a japanese salt-tolerant pumpkin cultivar 'king shintosa' (cucurbita maxima x c. moschata) as rootstock and a cucumber (cucumis sativus) cultivar 'xintaimici' as cion, this paper studied the temporal dynamics of different form polyamines contents in the leaves of grafted and own-rooted cucumber plants under 100 mmol x l(-1) nacl stress. the results showed that the free putrescence (put) content of graftedplant leaves (g2) was significantly higher than that of own-rooted plant leaves (o2), e ... | 2007 | 17615880 |
| reciprocal phosphorylation and glycosylation recognition motifs control ncapp1 interaction with pumpkin phloem proteins and their cell-to-cell movement. | in plants, cell-to-cell trafficking of non-cell-autonomous proteins (ncaps) involves protein-protein interactions, and a role for posttranslational modification has been implicated. in this study, proteins contained in pumpkin (cucurbita maxima cv big max) phloem sap were used as a source of ncaps to further explore the molecular basis for selective ncap trafficking. protein overlay assays and coimmunoprecipitation experiments established that phosphorylation and glycosylation, on both nicotiana ... | 2007 | 17601822 |
| isolation and expression of ga 2-oxidase2 in tomato. | ga 2-oxidases, a key enzyme involves ga biosynthesis, catalyze the degradation of active c(19)-gibberellins (gas) through 2-hydroxylation yields inactive ga product. searching public tomato database, the putative ga2ox2 sequences were assembled. we isolated a full-length ga2ox2 cdna with primers designed from the assembled sequence. this gene was designed as slga2ox2 (genbank accession no. ef017805). the full-length ga2ox2 gene contained a complete open reading frame (orf) of 1203 bp, which enco ... | 2007 | 17676476 |
| engineered resistance against proteinases. | exogenous proteinase inhibitors are valuable and economically interesting protective biotechnological tools. we examined whether small proteinase inhibitors when fused to a selected target protein can protect the target from proteolytic degradation without simultaneously affecting the function and activity of the target domain. two proteinase inhibitors were studied: a kazal-type silk proteinase inhibitor (spi2) from galleria mellonella, and the cucurbita maxima trypsin inhibitor i (cmti i). bot ... | 2007 | 17823663 |
| ethnomedicines used in trinidad and tobago for reproductive problems. | throughout history women have tried to control or enhance their fertility using herbal remedies, with various levels of societal support. caribbean folk medicine has been influenced by european folk medicine, either through the early spanish and french settlers or through the continuous immigration of spanish-speaking peoples from venezuela. some folk uses are ancient and were documented by galen and pliny the elder. | 2007 | 17362507 |
| oil and tocopherol content and composition of pumpkin seed oil in 12 cultivars. | twelve pumpkin cultivars (cucurbita maxima d.), cultivated in iowa, were studied for their seed oil content, fatty acid composition, and tocopherol content. oil content ranged from 10.9 to 30.9%. total unsaturated fatty acid content ranged from 73.1 to 80.5%. the predominant fatty acids present were linoleic, oleic, palmitic, and stearic. significant differences were observed among the cultivars for stearic, oleic, linoleic, and gadoleic acid content of oil. low linolenic acid levels were observ ... | 2007 | 17439238 |
| content and in-vitro accessibility of pro-vitamin a carotenoids from sri lankan cooked non-leafy vegetables and their estimated contribution to vitamin a requirement. | vitamin a deficiency is a public health problem in sri lanka, which affects especially pre-school children. carrots (daucus carota), pumpkins (cucurbita maxima), squashes (cucurbita moschata) and sweet potatoes (ipomoea batatas) of orange, yellow-fleshed varieties are good sources of provitamin a carotenoids, but have not been studied in sri lanka in terms of the food as eaten. the content of carotenoids in each preparation method and the in-vitro accessibility of beta-carotene and alpha-caroten ... | 2007 | 17852483 |
| nutritional and anti-nutritional compositions of cleome rutidosperma, lagenaria siceraria, and cucurbita maxima seeds from nigeria. | flours produced from the seeds of cleome rutidosperma (dc), lagenaria siceraria (mol.), and cucurbita maxima (duch) were evaluated for proximate and elemental compositions. preliminary phytochemical screening was also carried out on the seed flours, which revealed the presence of alkaloids, steroids, pentose, and reducing sugars in all the seed types. tannins, flavonoids, and cardiac glycosides were also detected in only c. rutidosperma seed. the results also showed that the seeds of c. rutidosp ... | 2007 | 18158851 |
| larvicidal, ovicidal and repellent activities of the leaf extract of two cucurbitacious plants against filarial vector culex quinquefasciatus (say) (diptera : culicidae). | the leaf extract of two cucurbitacious plants citrullus colocynthis and cucurbita maxima with different solvents viz., benzene, ethylacetate, petroleum ether and methanol were tested for larvicidal, ovicidal and repellent activities against the mosquito culex quinquefasciatus. larval mortality was observed and recorded after 24 h exposure period. the lc50 values of c. colocynthis were 61.72, 47.58, 66.92 and 118.74 ppm respectively. c. maxima shows the lc50 values of 123.02, 75.91, 117.73 and 17 ... | 2007 | 17568371 |
| flowering locus t protein may act as the long-distance florigenic signal in the cucurbits. | cucurbita moschata, a cucurbit species responsive to inductive short-day (sd) photoperiods, and zucchini yellow mosaic virus (zymv) were used to test whether long-distance movement of flowering locus t (ft) mrna or ft is required for floral induction. ectopic expression of ft by zymv was highly effective in mediating floral induction of long-day (ld)-treated plants. moreover, the infection zone of zymv was far removed from floral meristems, suggesting that ft transcripts do not function as the f ... | 2007 | 17540715 |
| coccinellids, aphids, and pollen in diversified vegetable fields with transgenic and isoline cultivars. | the influence of concurrent introduction of three transgenic vegetable cultivars on seasonal dynamics of coccinellids and their food, aphids and pollen, was examined within diversified farm systems practicing insect pest management in northeastern us agroecosystems. the transgenic cultivars used included sweet corn, potato, and winter squash, expressing cry1(a)b, cry3a, and plant viral coat proteins that target lepidoptera, coleoptera, and aphid-transmitted viruses, respectively. transgenic syst ... | 2007 | 20307238 |
| uptake, translocation, and accumulation of manufactured iron oxide nanoparticles by pumpkin plants. | rapid development and application of nanomaterials and nanotechnology make assessment of their potential health and environmental impacts on humans, non-human biota, and ecosystems imperative. here we show that pumpkin plants (cucurbita maxima), grown in an aqueous medium containing magnetite (fe3o4) nanoparticles, can absorb, translocate, and accumulate the particles in the plant tissues. these results suggest that plants, as an important component of the environmental and ecological systems, n ... | 2008 | 18528537 |
| pancreatic proteolytic enzymes of ostrich purified on immobilized protein inhibitors. characterization of a new form of chymotrypsin (chtr1). | four forms of chymotrypsin (chtr1, chtr2, chtr3, chtr4), one form of trypsin and one form of elastase were purified from a slightly alkaline extract of ostrich (struthio camelus) pancreas. the zymogens in the crude extract were activated with immobilized trypsin and then separated by affinity chromatography using immobilized inhibitors and ion exchange chromatography. one of the purified forms of chymotrypsin (chtr1) exhibited an unusual interaction with the highly selective protein trypsin inhi ... | 2008 | 18598777 |
| 1h-n.m.r. studies of squash seed trypsin inhibitor. | 1h-n.m.r. studies at 500 mhz have been performed on a trypsin inhibitor (cmti-iii) found in squash seed (cucurbita maxima). the sequential resonance assignments have been made using two-dimensional techniques. the chemical shifts for the assigned protons are reported at 30 degrees, ph 2.8 and form a basis for the determination of the solution structure of cmti-iii. analysis of the noe data, nh-alpha ch vicinal coupling constants and pattern of slowly exchanging amide protons indicates that the p ... | 2008 | 2613440 |
| hplc-dad-ms(n) characterisation of carotenoids from apricots and pumpkins for the evaluation of fruit product authenticity. | carotenoids including carotenoid esters from six apricot (prunus armeniaca l.) cultivars and from eight cultivars from three pumpkin species (cucurbita maxima duch., cucurbita pepo l., and cucurbita moschata duch.) were extracted without saponification, separated on a c-30 reversed-phase column and characterised by high-performance liquid chromatography/atmospheric pressure chemical ionisation-mass spectrometry (lc-ms). the predominant free carotenoids were quantified by hplc with diode array de ... | 2008 | 26049248 |
| exact and effective pair-wise potential for protein-ligand interactions obtained from a semiempirical energy partition. | in this work, the partition method introduced by carvalho and melo was used to study the complex between cucurbita maxima trypsin inhibitor (cmti-i) and glycerol at the am1 level. an effective potential, combining non-bonding and polarization plus charge transfer (plct) terms, was introduced to evaluate the magnitude of the interaction between each amino acid and the ligand. in this case study, the nonbonding-plct non-compensation characterizes the stabilization energy of the association process ... | 2008 | 19325823 |
| the phloem-delivered rna pool contains small noncoding rnas and interferes with translation. | in plants, the vascular tissue contains the enucleated sieve tubes facilitating long-distance transport of nutrients, hormones, and proteins. in addition, several mrnas and small interfering rnas/micrornas were shown to be delivered via sieve tubes whose content is embodied by the phloem sap (ps). a number of these phloem transcripts are transported from source to sink tissues and function at targeted tissues. to gain additional insights into phloem-delivered rnas and their potential role in sig ... | 2009 | 19261735 |
| a polypyrimidine tract binding protein, pumpkin rbp50, forms the basis of a phloem-mobile ribonucleoprotein complex. | rna binding proteins (rbps) are integral components of ribonucleoprotein (rnp) complexes and play a central role in rna processing. in plants, some rbps function in a non-cell-autonomous manner. the angiosperm phloem translocation stream contains a unique population of rbps, but little is known regarding the nature of the proteins and mrna species that constitute phloem-mobile rnp complexes. here, we identified and characterized a 50-kd pumpkin (cucurbita maxima cv big max) phloem rna binding pr ... | 2009 | 19122103 |
| evaluation of alternatives to methyl bromide in melon crops in guatemala. | the monoculture of melon in guatemala has caused the massive appearance of plants with an analogous syndrome for the well-known disease commonly called melon collapse, or vine decline, causing significant losses in crops. methyl bromide is commonly used to sterilize soil prior to planting in guatemala, but it must be phased out by 2015. the objective of this study was to evaluate the technique of grafting melon onto hybrids of cucurbita (cucurbita maxima x cucurbita moschata), as an alternative ... | 2009 | 18998189 |
| tryptophan exposure and accessibility in the chitooligosaccharide-specific phloem exudate lectin from pumpkin (cucurbita maxima). a fluorescence study. | the exposure and accessibility of the tryptophan residues in the chitooligosaccharide-specific pumpkin (cucurbita maxima) phloem exudate lectin (ppl) have been investigated by fluorescence spectroscopy. the emission lambda(max) of native ppl, seen at 338nm was red-shifted to 348nm upon denaturation by 6m gdn.hcl in the presence of 10mm beta-mercaptoethanol, indicating near complete exposure of the tryptophan residues to the aqueous medium, whereas a blue-shift to 335nm was observed in the presen ... | 2009 | 19700341 |
| antioxidative activity of water soluble polysaccharide in pumpkin fruits (cucurbita maxima duchesne). | we evaluated the antioxidative activity of a water soluble polysaccharide fraction (wsp) from pumpkin fruits (cucurbita maxima duchesne). in the wsp, dpph radical scavenging and superoxide dismutase-like activity increased depending on the total sugar content. furthermore, the wsp can serve as an inhibitor of ascorbic acid oxidation. the efficacy was also affected by the total sugar content. | 2009 | 19502750 |
| comparison of perimeter trap crop varieties: effects on herbivory, pollination, and yield in butternut squash. | perimeter trap cropping (ptc) is a method of integrated pest management (ipm) in which the main crop is surrounded with a perimeter trap crop that is more attractive to pests. blue hubbard (cucurbita maxima duch.) is a highly effective trap crop for butternut squash (c. moschata duch. ex poir) attacked by striped cucumber beetles (acalymma vittatum fabricius), but its limited marketability may reduce adoption of ptc by growers. research comparing border crop varieties is necessary to provide opt ... | 2009 | 19791616 |
| allergy from giant pumpkin (cucurbita maxima) is not a fairy tale. | 2009 | 19732049 | |
| using trap crops for control of acalymma vittatum (coleoptera: chrysomelidae) reduces insecticide use in butternut squash. | striped cucumber beetle, acalymma vittatum f., is the primary insect pest of cucurbit crops in the northeastern united states. adult beetles colonize squash crops from field borders, causing feeding damage at the seedling stage and transmitting bacterial wilt erwinia tracheiphila hauben et al. 1999. conventional control methods rely on insecticide applications to the entire field, but surrounding main crops with a more attractive perimeter could reduce reliance on insecticides. a. cittatum shows ... | 2009 | 19610425 |
| buttercup squash provides a marketable alternative to blue hubbard as a trap crop for control of striped cucumber beetles (coleoptera: chrysomelidae). | winter squash is a vital agricultural commodity worldwide. in the northeastern united states, the primary insect pest is the striped cucumber beetle, acalymma vittatum f. using a blue hubbard squash (cucurbita maxima duchesne) perimeter trap crop system can reduce insecticide use by >90% in butternut squash (c. moschata poir), the primary winter squash grown in this region. despite the savings in insecticide costs, growers may be reluctant to give up field space for a perimeter crop of blue hubb ... | 2010 | 22182562 |
| differential responses in yield of pumpkin (cucurbita maxima l.) and nightshade (solanum retroflexum dun.) to the application of three animal manures. | crop responses to different manures differs considerably, however, the factors responsible for it have not been conclusively elucidated. consequently, this study examined the biomass response of cucurbita maxima and solanum retroflexum to application rates of chicken and kraal manures of cattle and goat, and soil factors related to salinity. the crops' biomass yield increased linearly with increase in application rates of kraal and chicken manures, but steeper in the latter. results showed that ... | 2010 | 19954967 |
| assessing risk to human health from tropical leafy vegetables grown on contaminated urban soils. | fifteen tropical leafy vegetable types were sampled from farmers' gardens situated on nine contaminated sites used to grow vegetables for commercial or subsistence consumption in and around kampala city, uganda. trace metal concentrations in soils were highly variable and originated from irrigation with wastewater, effluent discharge from industry and dumping of solid waste. metal concentrations in the edible shoots of vegetables also differed greatly between, and within, sites. gynandropsis gyn ... | 2010 | 20739044 |
| pumpkin eif5a isoforms interact with components of the translational machinery in the cucurbit sieve tube system. | in yeast, eif5a, in combination with eef2, functions at the translation step, during the protein elongation cycle. this result is of significance with respect to functioning of the enucleate sieve tube system, as eif5a was recently detected in cucurbita maxima (pumpkin) phloem sap. in the present study, we further characterized four cmeif5a isoforms, encoding three proteins, all of which were present in the phloem sap. although hypusination of cmeif5a was not necessary for entry into the sieve e ... | 2010 | 20807213 |
| carotenoid content and in vitro bioaccessibility of lutein in some leafy vegetables popular in sri lanka. | lutein is one of the major carotenoids in green leafy vegetables (glvs), which show a marked antioxidant activity. the study was carried out to identify and quantify the carotenoid content of selected sri lankan glvs. rp-hplc with photodiode array detection method was followed for the separation, identification and quantification of carotenoids. among the vegetables analyzed, ipomoea batatas leaves showed the highest beta-carotene content (743.9+/-35.0 microg/g dry weight (dw)) while syngonium a ... | 2010 | 20651462 |
| extraction of ascorbate oxidase from cucurbita maxima by continuous process in perforated rotating disc contactor using aqueous two-phase systems. | the ascorbate oxidase is the enzyme used to determine the content of ascorbic acid in the pharmaceutical and food industries and clinics analyses. the techniques currently used for the purification of this enzyme raise its production cost. thus, the development of alternative processes and with the potential to reduce costs is interesting. the application of aqueous two-phase system is proposed as an alternative to purification because it enables good separation of biomolecules. the objective of ... | 2010 | 19412579 |
| sieve tube geometry in relation to phloem flow. | sieve elements are one of the least understood cell types in plants. translocation velocities and volume flow to supply sinks with photoassimilates greatly depend on the geometry of the microfluidic sieve tube system and especially on the anatomy of sieve plates and sieve plate pores. several models for phloem translocation have been developed, but appropriate data on the geometry of pores, plates, sieve elements, and flow parameters are lacking. we developed a method to clear cells from cytopla ... | 2010 | 20354199 |
| random estimate the values of seed oil of cucurbita maxima by refractive index method. | the crude oil having lower iodine and free fatty acids values has aamdosha properties. these properties are present due to toxic and anti-toxic compounds. these compounds can be harmful for the special diseases and may be unsaturated, saturated, open chain etc. the adulteration can take part as catalytic action for the toxic effect for the special diseases. toxic properties of oils are removed by different ingrediants and methods. c. maxima seed tail (mst) is used with food and medicine. the pre ... | 2010 | 22131677 |
| rapid affinity-purification and physicochemical characterization of pumpkin (cucurbita maxima) phloem exudate lectin. | the chito-oligosaccharide-specific lectin from pumpkin (cucurbita maxima) phloem exudate has been purified to homogeneity by affinity chromatography on chitin. after sds/page in the presence of 2-mercaptoethanol, the pumpkin phloem lectin yielded a single band corresponding to a molecular mass of 23.7 kda, whereas esi-ms (electrospray ionization ms) gave the molecular masses of the subunit as 24645 da. analysis of the cd spectrum of the protein indicated that the secondary structure of the lecti ... | 2010 | 19735229 |
| response of epilachna paenulata to two flavonoids, pinocembrin and quercetin, in a comparative study. | we examined the effects of the flavonoids pinocembrin and quercetin on the feeding behavior, survival, and development of the cucurbitaceae pest epilachna paenulata (coleoptera: coccinellidae). in no-choice experiments, 48 hr-consumption of cucurbita maxima duch. leaves treated with pinocembrin at 1, 5, and 50 microg/cm(2) was less than one third of that for leaves treated with 0.1 microg/cm(2) of pinocembrin or untreated leaves. larvae stopped feeding after 9 days of high doses of pinocembrin ( ... | 2010 | 20589417 |
| remote-controlled stop of phloem mass flow by biphasic occlusion in cucurbita maxima. | the relationships between damage-induced electropotential waves (epws), sieve tube occlusion, and stop of mass flow were investigated in intact cucurbita maxima plants. after burning leaf tips, epws propagating along the phloem of the main vein were recorded by extra- and intracellular microelectrodes. the respective epw profiles (a steep hyperpolarization/depolarization peak followed by a prolonged hyperpolarization/depolarization) probably reflect merged action and variation potentials. a few ... | 2010 | 20584788 |
| changes in carotenoids during processing and storage of pumpkin puree. | changes in the contents of carotenoids and their true retentions (% tr) during the production of puree of cucurbita moschata 'menina brasileira' and of cucurbita maxima 'exposição' pumpkins and the stability of such compounds during 180days of storage were monitored by liquid chromatography coupled with a photodiode array detector. cooking caused higher losses than commercial sterilisation. high losses of xanthophylls such as lutein and violaxanthin were noted during processing and storage of pu ... | 2011 | 25214348 |
| comparison of waste pumpkin material and its potential use in extruded snack foods. | material was produced from crown pumpkin (cucurbita maxima) processed from fractions of the fruit which are regarded as waste stream products (peel, flesh and seed). the flour from the three different fractions (peel, flesh and seed) of crown pumpkin flour was incorporated into an extruded snack product formulation at levels 10%, 30% and 50% (w/w with corn grit) and processed in a twin-screw extruder to make 10 expanded snack products. proximate analysis was carried out to determine the nutritio ... | 2011 | 21813596 |
| an in vitro evaluation of anthelmintic activity of zingiber zerumbet rhizomes and cucurbita maxima seeds on pheretima posthuma model: a comparative study. | 2011 | 21687369 | |
| effects of cooking on the cell walls (dietary fiber) of 'scarlet warren' winter squash ( cucurbita maxima ) studied by polysaccharide linkage analysis and solid-state (13)c nmr. | cell wall polysaccharides of 'scarlet warren' winter squash ( cucurbita maxima ) were investigated before and after thermal processing. linkage analysis of polysaccharides was done by gas chromatography coupled to mass spectrometry (gc-ms). the linkage analysis showed the cell wall polysaccharide compositions of raw and cooked squash were similar. the total pectic polysaccharides (galacturonan, rhamnogalacturonan, arabinan, and arabinogalactan) contents of the cell walls of both raw and cooked s ... | 2011 | 21604813 |
| cmrbp50 protein phosphorylation is essential for assembly of a stable phloem-mobile high-affinity ribonucleoprotein complex. | rna-binding proteins (rbps) form ribonucleoprotein (rnp) complexes that play crucial roles in rna processing for gene regulation. the angiosperm sieve tube system contains a unique population of transcripts, some of which function as long-distance signaling agents involved in regulating organ development. these phloem-mobile mrnas are translocated as rnp complexes. one such complex is based on a phloem rbp named cucurbita maxima rna-binding protein 50 (cmrbp50), a member of the polypyrimidine tr ... | 2011 | 21572046 |
| isothermal titration calorimetric and computational studies on the binding of chitooligosaccharides to pumpkin (cucurbita maxima) phloem exudate lectin. | the interaction of chitooligosaccharides [(glcnac)(2-6)] with pumpkin phloem exudate lectin (ppl) was investigated by isothermal titration calorimetry and computational methods. the dimeric ppl binds to (glcnac)(3-5) with binding constants of 1.26-1.53 × 10(5) m(-1) at 25 °c, whereas chitobiose exhibits approximately 66-fold lower affinity. interestingly, chitohexaose shows nearly 40-fold higher affinity than chitopentaose with a binding constant of 6.16 × 10(6) m(-1). the binding stoichiometry ... | 2011 | 21405077 |
| differential spatial expression of a- and b-type cdks, and distribution of auxins and cytokinins in the open transverse root apical meristem of cucurbita maxima. | aside from those on arabidopsis, very few studies have focused on spatial expression of cyclin-dependent kinases (cdks) in root apical meristems (rams), and, indeed, none has been undertaken for open meristems. the extent of interfacing between cell cycle genes and plant growth regulators is also an increasingly important issue in plant cell cycle studies. here spatial expression/localization of an a-type and b-type cdk, auxin and cytokinins are reported in relation to the hitherto unexplored an ... | 2011 | 20601387 |
| vascular expression in arabidopsis is predicted by the frequency of ct/ga-rich repeats in gene promoters. | phloem-transported signals play an important role in regulating plant development and in orchestrating responses to environmental stimuli. among such signals, phloem-mobile rnas have been shown to play an important role as long-distance signaling agents. at maturity, angiosperm sieve elements are enucleate, and thus transcripts in the phloem translocation stream probably originate from the nucleate companion cells. in the present study, a pumpkin (cucurbita maxima) phloem transcriptome was used ... | 2011 | 21435051 |
| sodium and chloride exclusion and retention by non-grafted and grafted melon and cucurbita plants. | the effects of grafting on na and cl(-) uptake and distribution in plant tissues were quantified in a greenhouse experiment using six combinations of melon (cucumis melo l. cv. arava) and pumpkin (cucurbita maxima duchesne×cucurbita moschata duchesne cv. tz-148): non-grafted, self-grafted, melons grafted on pumpkins, and pumpkins grafted on melons. total na concentration in shoots of plants with pumpkin or melon rootstocks was <60 mmol kg(-1) and >400 mmol kg(-1), respectively, regardless of the ... | 2011 | 20729482 |
| agrobacterium-mediated transformation of kabocha squash (cucurbita moschata duch) induced by wounding with aluminum borate whiskers. | an efficient genetic transformation method for kabocha squash (cucurbita moschata duch cv. heiankogiku) was established by wounding cotyledonary node explants with aluminum borate whiskers prior to inoculation with agrobacterium. adventitious shoots were induced from only the proximal regions of the cotyledonary nodes and were most efficiently induced on murashige-skoog agar medium with 1 mg/l benzyladenine. vortexing with 1% (w/v) aluminum borate whiskers significantly increased agrobacterium i ... | 2011 | 21400224 |
| microplot evaluation of rootstocks for control of meloidogyne incognita on grafted tomato, muskmelon, and watermelon. | microplot experiments were conducted over two years (four growing seasons) to evaluate meloidogyne incognita resistance in rootstocks used for grafted tomato (solanum lycopersicum), muskmelon (cucumis melo), and watermelon (citrullus lanatus). three tomato rootstocks; 'tx301', 'multifort', and 'aloha', were tested in addition to the nongrafted scion, 'florida-47'. two muskmelon rootstocks; cucumis metuliferus and 'tetsukabuto' (cucurbita maxima × cucurbita moschata) were evaluated with the nongr ... | 2011 | 23431109 |
| sieve element occlusion (seo) genes encode structural phloem proteins involved in wound sealing of the phloem. | the sieve element occlusion (seo) gene family originally was delimited to genes encoding structural components of forisomes, which are specialized crystalloid phloem proteins found solely in the fabaceae. more recently, seo genes discovered in various non-fabaceae plants were proposed to encode the common phloem proteins (p-proteins) that plug sieve plates after wounding. we carried out a comprehensive characterization of two tobacco (nicotiana tabacum) seo genes (ntseo). reporter genes controll ... | 2012 | 22733783 |
| looking deep inside: detection of low-abundance proteins in leaf extracts of arabidopsis and phloem exudates of pumpkin. | the field of proteomics suffers from the immense complexity of even small proteomes and the enormous dynamic range of protein concentrations within a given sample. most protein samples contain a few major proteins, which hamper in-depth proteomic analysis. in the human field, combinatorial hexapeptide ligand libraries (cpll; such as proteominer) have been used for reduction of the dynamic range of protein concentrations; however, this technique is not established in plant research. in this work, ... | 2012 | 22555880 |
| [salt stress tolerance of cucumber-grafted rootstocks]. | taking 4 different cucurbita maxima x c. moschata rootstocks for cucumber (cucumis sativus) as test materials, a solution culture experiment was conducted to study their growth and antioxidative enzyme activities under the stresses of ca(no3)2 and nacl, with the salt stress tolerance of the rootstocks evaluated by subordinate function. at 30 mmol x l(-1) of ca (no3)2 or 45 mmol x l(-1) of nacl, the growth of the rootstock seedlings was improved; but at 60 and 120 mmol x l(-1) of ca(no3)2 or 90 a ... | 2012 | 22919842 |
| the origin and composition of cucurbit "phloem" exudate. | cucurbits exude profusely when stems or petioles are cut. we conducted studies on pumpkin (cucurbita maxima) and cucumber (cucumis sativus) to determine the origin and composition of the exudate. morphometric analysis indicated that the exudate is too voluminous to derive exclusively from the phloem. cold, which inhibits phloem transport, did not interfere with exudation. however, ice water applied to the roots, which reduces root pressure, rapidly diminished exudation rate. sap was seen by micr ... | 2012 | 22331409 |
| overexpression of arabidopsis plasmodesmata germin-like proteins disrupts root growth and development. | in plants, a population of non-cell-autonomous proteins (ncaps), including numerous transcription factors, move cell to cell through plasmodesmata (pd). in many cases, the intercellular trafficking of these ncaps is regulated by their interaction with specific pd components. to gain further insight into the functions of this ncap pathway, coimmunoprecipitation experiments were performed on a tobacco (nicotiana tabacum) plasmodesmal-enriched cell wall protein preparation using as bait the ncap, p ... | 2012 | 22960910 |
| accumulation of weathered p,p'-ddts in grafted watermelon. | the grafting of melon plants onto cucurbit rootstocks is a common commercial practice in many parts of the world. however, certain cucurbits have been shown to accumulate large quantities of weathered persistent organic pollutants from the soil, and the potential contamination of grafted produce has not been thoroughly evaluated. large pot and field experiments were conducted to assess the effect of grafting on accumulation of weathered ddx (the sum of p,p'-ddt, p,p'-ddd, and p,p'-dde) from soil ... | 2012 | 22224752 |
| in vivo quantification of cell coupling in plants with different phloem-loading strategies. | uptake of photoassimilates into the leaf phloem is the key step in carbon partitioning and phloem transport. symplasmic and apoplasmic loading strategies have been defined in different plant taxa based on the abundance of plasmodesmata between mesophyll and phloem. for apoplasmic loading to occur, an absence of plasmodesmata is a sufficient but not a necessary criterion, as passage of molecules through plasmodesmata might well be blocked or restricted. here, we present a noninvasive, whole-plant ... | 2012 | 22422939 |
| stamen-derived bioactive gibberellin is essential for male flower development of cucurbita maxima l. | gibberellin (ga) signalling during pumpkin male flower development is highly regulated, including biosynthetic, perception, and transduction pathways. ga 20-oxidases, 3-oxidases, and 2-oxidases catalyse the final part of ga synthesis. additionally, 7-oxidase initiates this part of the pathway in some cucurbits including cucurbita maxima l. (pumpkin). expression patterns for these ga-oxidase-encoding genes were examined by competitive reverse transcription-pcr (rt-pcr) and endogenous ga levels we ... | 2012 | 22268154 |
| quantification of plant cell coupling with three-dimensional photoactivation microscopy. | plant cells are directly connected by plasmodesmata that form channels through the cell wall and enable the intercellular movement of cytosolic solutes, membrane lipids and signalling molecules. transport through plasmodesmata is regulated not only by a fixed size-exclusion limit, but also by physiological and pathological adaptation. to understand plant cell communication, carbon allocation and pathogen attack, the capacities for a specific molecule to pass a specific cell-wall interface is an ... | 2012 | 22171617 |
| carotenoid accumulation and carotenogenic gene expression during fruit development in novel interspecific inbred squash lines and their parents. | carotenoid levels and composition during squash fruit development were compared in cucurbita moschata , cucurbita maxima , and two lines of their interspecific inbred lines, namely, maxchata1 and maxchata2. eight genes associated with carotenoid biosynthesis were analyzed by quantitative rt-pcr. the two squash species and their interspecific inbred lines exhibited different qualitative and quantitative carotenoid profiles and regulatory mechanisms. c. moschata had the lowest total carotenoid con ... | 2012 | 22574777 |
| transgenic tobacco plants as production platform for biologically active human interleukin 2 and its fusion with proteinase inhibitors. | transgenic plants offer a low-cost approach for the production of pharmaceutically important and commercially valuable recombinant proteins. our studies were focused on the plant-based production of human interleukin 2 (hil-2) and its fusion with proteinase inhibitors, either spi2 from galleria mellonella or cmti from cucurbita maxima. finally, five plant expression cassettes were obtained. three of them contained the single cdna encoding cmti i, spi2 and hil-2, respectively, while two of them c ... | 2012 | 22564275 |
| dissoconiaceae associated with sooty blotch and flyspeck on fruits in china and the united states. | zasmidium angulare, a novel species of mycosphaerellaceae, and several novel taxa that reside in dissoconiaceae, were identified from a collection of apples and cucurbita maxima (cv. blue hubbard) from china and the usa that exhibited sooty blotch and flyspeck (sbfs) signs on their host substrata. morphology on fruit surfaces and in culture, and phylogenetic analyses of the nuclear ribosomal dnas 28s and internal transcribed spacer regions, as well as partial translation elongation factor 1-alph ... | 2012 | 23105157 |
| deciphering systemic wound responses of the pumpkin extrafascicular phloem by metabolomics and stable isotope-coded protein labeling. | in cucurbits, phloem latex exudes from cut sieve tubes of the extrafascicular phloem (efp), serving in defense against herbivores. we analyzed inducible defense mechanisms in the efp of pumpkin (cucurbita maxima) after leaf damage. as an early systemic response, wounding elicited transient accumulation of jasmonates and a decrease in exudation probably due to partial sieve tube occlusion by callose. the energy status of the efp was enhanced as indicated by increased levels of atp, phosphate, and ... | 2012 | 23085839 |
| comparative analysis of cells and proteins of pumpkin plants for the control of fruit size. | common pumpkin plants (cucurbita maxima) produce fruits of 1-2 kg size on the average, while special varieties of the same species called atlantic giant are known to produce a huge fruit up to several hundred kilograms. as an approach to determine the factors controlling the fruit size in c. maxima, we cultivated both ag and control common plants, and found that both the cell number and cell sizes were increased in a large fruit while dna content of the cell did not change significantly. we also ... | 2012 | 22677066 |
| high efficiency joint cze determination of sugars and acids in vegetables and fruits. | in this work, an improved ce method for the medium-throughput determination of main organic acids (oxalate, malate, citrate), the amino acid glutamate and the sugars fructose, glucose and sucrose in several food matrices is described. these compounds have been identified as key components in the taste intensity of fruit and vegetable crops. using a running buffer with 20 mm 2,6-pyridine dicarboxylic acid ph 12.1 and 0.1% hexadimethrine bromide, replacing it every 5 h to avoid ph decrease, and op ... | 2012 | 22887163 |
| ascorbic acid biosensor using ascorbate oxidase immobilized on alkylamine glass beads. | a biosensor for ascorbic acid based on enzyme kinetics of ascorbate oxidase (e.c.1.10.3.3) was developed. the enzyme was extracted from cucurbita maxima, or jerimun and immobilized by covalent bounding, using glutaradehyde as a bifunctional agent, on alkylamine glass beads, with and without enzyme active site protection. a low-cost, home-made oxygen electrode was applied as a transducer. the system has sensitivity from 62.5 up to 500 microm of ascorbic acid with satisfactory operation for more t ... | 2013 | 1416950 |
| content of bioactive compounds and antioxidant capacity of pumpkin puree enriched with japanese quince, cornelian cherry, strawberry and apples. | when evaluated in terms of taste, smell or active ingredients, pumpkin in itself is not very attractive as a raw material. hence, it is recommended to blend pumpkin with other fruits, which are aromatic, have a defined taste, and contain a large quantity of active ingredients and organic acids to improve its palatibility. | 2013 | 22232528 |
| three new multiflorane-type triterpenes from pumpkin (cucurbita maxima) seeds. | three new multiflorane-type triterpenes; 7a-methoxymultiflor-8-ene-3a,29-diol 3-acetate-29-benzoate (1), 7-oxomultiflor-8-ene-3a,29-diol 3-acetate-29-benzoate (2), and multiflora-7,9(11)-diene-3a,29-diol 3-p-hydroxybenzoate-29-benzoate (3), were isolated from seeds of cucurbita maxima, along with three known compounds. compound 3 and multiflora-7,9(11)-diene-3a-29-diol 3-benzoate (5) exhibited potent inhibitory effects on melanogenesis, with low cytotoxicities, and 2 exhibited single-digit micro ... | 2013 | 23673529 |
| interaction of xylem and phloem during exudation and wound occlusion in cucurbita maxima. | collection of cucurbit exudates from cut petioles has been a powerful tool for gaining knowledge on phloem sap composition without full notion of the complex exudation mechanism. only few publications explicitly mentioned that exudates were collected from the basal side of the cut, which exudes more copiously than the apical side. this is surprising since only exudation from the apical side is supposedly driven by phloem pressure gradients. composition of carbohydrates and ph values at both woun ... | 2013 | 22765252 |
| metabolites of 2,4,4'-tribrominated diphenyl ether (bde-28) in pumpkin after in vivo and in vitro exposure. | there is currently limited knowledge on pbde metabolism in plants although they could play an important role in the environmental transformation of these persistent organic pollutants. in this study, pumpkin (cucurbita maxima × c. moschata) was chosen as the model to understand the fate of bde-28 in plants. meo-tri-bdes, oh-tri-bdes, and oh-tri-bdes were found as metabolites in plant samples of both in vivo hydroponic and in vitro tissue culture exposure. three meo-tri-bdes were further identifi ... | 2013 | 24191731 |
| pumpkin (cucurbita maxima) seed proteins: sequential extraction processing and fraction characterization. | seed proteins extracted from tunisian pumpkin seeds ( cucurbita maxima ) were investigated for their solubility properties and sequentially extracted according to the osborne procedure. the solubility of pumpkin proteins from seed flour was greatly influenced by ph changes and ionic strength, with higher values in the alkaline ph regions. it also depends on the seed defatting solvent. protein solubility was decreased by using chloroform/methanol (cm) for lipid extraction instead of pentane (p). ... | 2013 | 23869935 |
| in vivo metabolism of 2,2',4,4'-tetrabromodiphenyl ether (bde-47) in young whole pumpkin plant. | polybrominated diphenyl ethers (pbdes) are widely distributed persistent organic pollutants. in vitro and in vivo research using various animal models have shown that pbdes might be transformed to hydroxylated pbdes, but there are few studies on in vivo metabolism of pbdes by intact whole plants. in this research, pumpkin plants (cucurbita maxima × c. moschata) were hydroponically exposed to 2,2',4,4'-tetrabromodiphenyl ether (bde-47). a debromination product (bde-28) and four hydroxylated metab ... | 2013 | 23510101 |
| antioxidant enzymatic activities and gene expression associated with heat tolerance in the stems and roots of two cucurbit species ("cucurbita maxima" and "cucurbita moschata") and their interspecific inbred line "maxchata". | the elucidation of heat tolerance mechanisms is required to combat the challenges of global warming. this study aimed to determine the antioxidant enzyme responses to heat stress, at the enzymatic activity and gene expression levels, and to investigate the antioxidative alterations associated with heat tolerance in the stems and roots of squashes using three genotypes differing in heat tolerance. plants of heat-tolerant "c. moschata", thermolabile "c. maxima" and moderately heat-tolerant intersp ... | 2013 | 24336062 |
| characteristics of antioxidant activity and composition of pumpkin seed oils in 12 cultivars. | the objective of this study was to determine the antioxidant properties, and provide characteristics, of the oil obtained from the seeds of 12 pumpkin varieties belonging to the species cucurbita maxima duch. and cucurbita pepo l. another objective was to establish which of the two extracting agents, ethanol or methanol, is more effective. the seeds of the pumpkin varieties examined differ in chemical composition and antioxidant activity. the seeds of the cultivars belonging to the species c. ma ... | 2013 | 23561092 |
| indigenous and traditional plants: south african parents' knowledge, perceptions and uses and their children's sensory acceptance. | the dietary shift from indigenous and traditional plants (itps) to cash crops and exotic plant food sources increases the risk of malnutrition and other nutrition-related non-communicable diseases, especially in poor rural communities. farm communities in south africa have been associated with poor nutritional status and extreme poverty. itps have been found to be affordable sources of several micronutrients. however, knowledge of and the use of these plants are declining, and little is known ab ... | 2013 | 24274394 |
| characterization of the pumpkin translationally-controlled tumor protein cmtctp. | in higher plants, the phloem plays a central role in the delivery of nutrients and signals from source to sink tissues. these signals likely coordinate different aspects of plant development, as well as its response to environmental cues. although some phloem-transported proteins and rnas may function as signaling molecules in plants, their mode of action remains poorly understood. previous analysis of transcripts from cmv-infected pumpkin (cucurbita maxima cv big max) identified a translational ... | 2013 | 24065051 |
| overexpression of the pumpkin (cucurbita maxima) 16 kda phloem protein cmpp16 increases tolerance to water deficit. | the phloem plays an important role in the delivery of nutrients and signals between photosynthetic to heterotrophic tissues. proteins and rnas in the phloem translocation stream may have an important role in maintaining the integrity of the sieve tube system, as well as in long-distance signaling. cmpp16 is a pumpkin phloem protein, which has been shown to bind rna in a non-sequence specific manner, and move it cell-to-cell and conceivably, long-distance. the protein and rna are found in both co ... | 2014 | 25482781 |
| mutational analysis of the pumpkin (cucurbita maxima) phloem exudate lectin, pp2 reveals ser-104 is crucial for carbohydrate binding. | the pumpkin phloem lectin (pp2) is an rna-binding, defense-related, chitooligosaccharide-specific, homodimeric lectin of mr 48 kda expressed at high concentrations in the sieve elements and companion cells of pumpkin (cucurbita maxima). in the present study, pp2 was expressed in the methylotrophic yeast pichia pastoris with the saccharomyces α-factor sequence to direct the recombinant protein into the secretory pathway as a prerequisite for unimpaired folding and posttranslational glycosylation ... | 2014 | 24950405 |
| bioactivities of some essential oils against the camel nasal botfly, cephalopina titillator. | nasopharyngeal myiasis of camels is caused by the larvae of cephalopina titillator. we determined the efficacy of essential oils (eos) of pumpkin, cucurbita maxima; lupinus, lupinus luteus; garlic oil, allium sativum; and peppermint, mentha piperita, against the third larval stage of c. titillator using larval immersion tests. the positive control group was treated with ivermectin and the negative control one was treated with distilled water and few drops of tween 80. larvae were reared until ad ... | 2014 | 24276644 |
| structural divergence of plant tctps. | the translationally controlled tumor protein (tctp) is a highly conserved protein at the level of sequence, considered to play an essential role in the regulation of growth and development in eukaryotes. however, this function has been inferred from studies in a few model systems, such as mice and mammalian cell lines, drosophila and arabidopsis. thus, the knowledge regarding this protein is far from complete. in the present study bioinformatic analysis showed the presence of one or more tctp ge ... | 2014 | 25120549 |
| wheat bread with pumpkin (cucurbita maxima l.) pulp as a functional food product. | in this study, a new application of pumpkin pulp in bread production is shown. the aim of this work is to determine the influence of the addition of fresh pumpkin pulp directly into wheat flour on physical, sensorial and biological properties of bread. the bioaccessibility of active compounds was also studied. an increase in the addition of pumpkin pulp from 5 to 20% (converted to dry matter) caused a decrease of bread volume and increase of crumb hardness and cohesiveness. the sensory character ... | 2014 | 27904316 |
| characteristics of organic acids in the fruit of different pumpkin species. | the aim of the research was to determine the composition of organic acids in fruit of different cultivars of three pumpkin species. the amount of acids immediately after fruit harvest and after 3 months of storage was compared. the content of organic acids in the examined pumpkin cultivars was assayed using the method of high performance liquid chromatography (hplc). three organic acids (citric acid, malic acid, and fumaric acid) were identified in the cultivars, whose content considerably varie ... | 2014 | 24262577 |
| 11s storage globulin from pumpkin seeds: regularities of proteolysis by papain. | limited proteolysis of the α- and β-chains and deep cleavage of the αβ-subunits by the cooperative (one-by-one) mechanism was observed in the course of papain hydrolysis of cucurbitin, an 11s storage globulin from seeds of the pumpkin cucurbita maxima. an independent analysis of the kinetics of the limited and cooperative proteolyses revealed that the reaction occurs in two successive steps. in the first step, limited proteolysis consisting of detachments of short terminal peptides from the α- a ... | 2014 | 25365492 |
| a review on omega-3 and omega-6 essential fatty acids: uses, benefits and their availability in pumpkins (cucurbita maxima) seed and desert dates (balanites aegyptiaca) seed kernel oils. | 2014 | 26027166 | |
| microwave-assisted aqueous enzymatic extraction of oil from pumpkin seeds and evaluation of its physicochemical properties, fatty acid compositions and antioxidant activities. | microwave-assisted aqueous enzymatic extraction (maaee) of pumpkin seed oil was performed in this study. an enzyme cocktail comprised of cellulase, pectinase and proteinase (w/w/w) was found to be the most effective in releasing oils. the highest oil recovery of 64.17% was achieved under optimal conditions of enzyme concentration (1.4%, w/w), temperature (44°c), time (66 min) and irradiation power (419w). moreover, there were no significant variations in physicochemical properties of maaee-extra ... | 2014 | 24206680 |
| systemic delivery of sirna in pumpkin by a plant phloem small rna-binding protein 1-ribonucleoprotein complex. | in plants, the vascular system, specifically the phloem, functions in delivery of small rna (srna) to exert epigenetic control over developmental and defense-related processes. although the importance of systemic srna delivery has been established, information is currently lacking concerning the nature of the protein machinery involved in this process. here, we show that a phloem small-rna binding protein 1 (psrp1) serves as the basis for formation of an srna ribonucleoprotein complex (srnpc) th ... | 2014 | 25227635 |
| description of ektaphelenchoides fuchsi n. sp. (nematoda: ektaphelenchinae) from western iran. | ektaphelenchoides fuchsi n. sp., recovered from a soil sample around the rhizosphere of cucurbita maxima in western iran, is described and illustrated based on morphological and molecular characters. the new species is characterized by its body length of 529-712 µm, continuous lip region, lateral fields with three incisures, total stylet length of 14-18 µm with rounded basal knobs, excretory pore 70-84 µm and hemizonid 87-96 µm from the anterior end, post-uterine sac short, 6-9 µm long in female ... | 2014 | 25112260 |
| pumpkin seed oil extracted from cucurbita maxima improves urinary disorder in human overactive bladder. | the pumpkin seed oil obtained from cucurbita pepo has been shown to be useful for the treatment of nocturia in patients with urinal disorders in several western countries. in this study, we evaluated the effect of the pumpkin seed oil from cucurbita maxima on urinary dysfunction in human overactive bladder (oab). forty-five subjects were enrolled in this study. an extract of pumpkin seed oil from c. maxima (10 g of oil/day) was orally administrated for 12 weeks. after 6 and 12 weeks, urinary fun ... | 2014 | 24872936 |
| three new triterpene esters from pumpkin (cucurbita maxima) seeds. | three new multiflorane-type triterpene esters, i.e. 7α-hydroxymultiflor-8-ene-3α,29-diol 3-acetate-29-benzoate (1), 7α-methoxymultiflor-8-ene-3α,29-diol 3,29-dibenzoate (2), and 7β-methoxymultiflor-8-ene-3α,29-diol 3,29-dibenzoate (3), were isolated from seeds of cucurbita maxima, along with the known compound, multiflora-7,9(11)-diene-3α,29-diol 3,29-dibenzoate (4). compound 1 exhibited melanogenesis inhibitory activities comparable with those of arbutin. in cytotoxicity assays, compounds 1 and ... | 2014 | 24743937 |
| direct and individual analysis of stress-related phytohormone dispersion in the vascular system of cucurbita maxima after flagellin 22 treatment. | • the stress-related phytohormones, salicylic acid (sa) and abscisic acid (aba), and the three jasmonates, jasmonic acid (ja), cis-12-oxo-phytodienoic acid (cis-opda), and (+)-7-iso-jasmonoyl-l-isoleucine (ja-ile), were investigated in phloem and xylem exudates of cucurbita maxima. • phloem and xylem exudates were separately collected and analysed via liquid chromatography-mass spectrometry. • we show direct evidence for all three jasmonates, aba, and sa in both phloem and xylem exudates of c. m ... | 2014 | 24387138 |
| regulatory control of carotenoid accumulation in winter squash during storage. | storage promotes carotenoid accumulation and converts amylochromoplasts into chromoplasts in winter squash. such carotenoid enhancement is likely due to continuous biosynthesis along with reduced turnover and/or enhanced sequestration. postharvest storage of fruits and vegetables is often required and frequently results in nutritional quality change. in this study, we investigated carotenoid storage plastids, carotenoid content, and its regulation during 3-month storage of winter squash butternu ... | 2014 | 25139277 |
| are trichomes involved in the biomechanical systems of cucurbita leaf petioles? | trichomes are involved in petiole movement and likely function as a part of the plant biomechanical system serving as an additional reservoir of hydrostatic pressure. the large, non-glandular trichomes on cucurbita petioles occur across collenchyma strands. time-lapse imaging was used to study the leaf reorientation of cucurbita maxima 'bambino' plants placed in horizontal position. the experiment comprised four variants of the large non-glandular petiole trichomes: (1) intact, (2) mechanically ... | 2015 | 26306605 |
| α-carotene and β-carotene content in raw and cooked pulp of three mature stage winter squash "type butternut". | winter squash "type butternut" is harvested in physiological ripening for better commercial distribution, when sensory and/or nutritional quality is not optimum for consumption. the objective of this study was to quantify the content of α-carotene, β-carotene, color and dry matter in the pulp of raw and microwave-cooked winter squash "type butternut" (variety cosmof1) in three states of commercial maturity. immature, mature, and very mature fruit, defined at the time of the harvest by the percen ... | 2015 | 28231218 |
| an acorn squash (cucurbita pepo ssp. ovifera) fruit and seed transcriptome as a resource for the study of fruit traits in cucurbita. | acorn squash (cucurbita pepo) is an iconic fall vegetable in the united states, known for its unique fruit shape and also prized for its culinary properties. little is known about the metabolism that underlies the development of fruit quality attributes such as color, sweetness, texture and nutritional qualities in acorn squash, or any other winter squash grown worldwide. to provide insight into winter squash fruit and seed development and add to the genomic resources in the cucurbita genus, rna ... | 2015 | 26504561 |
| a high-density genetic map for anchoring genome sequences and identifying qtls associated with dwarf vine in pumpkin (cucurbita maxima duch.). | pumpkin (cucurbita maxima duch.) is an economically important crop belonging to the cucurbitaceae family. however, very few genomic and genetic resources are available for this species. as part of our ongoing efforts to sequence the pumpkin genome, high-density genetic map is essential for anchoring and orienting the assembled scaffolds. in addition, a saturated genetic map can facilitate quantitative trait locus (qtl) mapping. | 2015 | 26704908 |
| characterization and pathogenicity of alternaria burnsii from seeds of cucurbita maxima (cucurbitaceae) in bangladesh. | in the course of survey of endophytic fungi from bangladesh pumpkin seeds in 2011~2012, two strains (cnu111042 and cnu111043) with similar colony characteristics were isolated and characterized by their morphology and by molecular phylogenetic analysis of the internal transcribed spacer, glyceraldehydes-3-phosphate dehydrogenase (gpd), and alternaria allergen a1 (alt a1) sequences. phylogenetic analysis of all three sequences and their combined dataset revealed that the fungus formed a subclade ... | 2015 | 26839497 |
| sequence variations in the fad2 gene in seeded pumpkins. | seeded pumpkins are important economic crops; the seeds contain various unsaturated fatty acids, such as oleic acid and linoleic acid, which are crucial for human and animal nutrition. the fatty acid desaturase-2 (fad2) gene encodes delta-12 desaturase, which converts oleic acid to linoleic acid. however, little is known about sequence variations in fad2 in seeded pumpkins. twenty-seven fad2 clones from 27 accessions of cucurbita moschata, cucurbita maxima, cucurbita pepo, and cucurbita ficifoli ... | 2015 | 26782391 |
| high throughput sequencing of small rnas in the two cucurbita germplasm with different sodium accumulation patterns identifies novel micrornas involved in salt stress response. | micrornas (mirnas), a class of small non-coding rnas, recognize their mrna targets based on perfect sequence complementarity. mirnas lead to broader changes in gene expression after plants are exposed to stress. high-throughput sequencing is an effective method to identify and profile small rna populations in non-model plants under salt stresses, significantly improving our knowledge regarding mirna functions in salt tolerance. cucurbits are sensitive to soil salinity, and the cucurbita genus is ... | 2015 | 26010449 |
| evaluation of cucurbita maxima extract against scopolamine-induced amnesia in rats: implication of tumour necrosis factor alpha. | cucurbita maxima (cm) seed oil is commonly used in indian folk medicine to treat various ailments. we have investigated the effect of cm seed oil on memory impairment induced by scopolamine in rats. male adult wistar rats were administered scopolamine 1 mg/kg body weight, i.p. or 1.25 mg/kg body weight, s.c. to induce memory impairment. the nootropic agent piracetam 100 mg/kg body weight, i.p. and cm seed oil 100 and 200 mg/kg body weight, p.o. were administered daily for five consecutive days. ... | 2015 | 25711042 |