Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| design, chemical synthesis and kinetic studies of trypsin chromogenic substrates based on the proteinase binding loop of cucurbita maxima trypsin inhibitor (cmti-iii). | a series of trypsin chromogenic substrates with formula: y-ala-x-abu-pro-lys-pna, where x = gly, ala, abu, val, leu, phe, ser, glu and y = ac, h; pna = p-nitroanilide was synthesized. the cucurbita maxima trypsin inhibitor cmti-iii molecule was used as a vehicle to design the trypsin substrates. to evaluate the influence of position p(4) on the substrate-enzyme interaction, kinetic parameters of newly synthesized substrates with bovine beta-trypsin were determined. the increasing hydrophobicity ... | 2000 | 10694481 |
| factors improving the accuracy of determination of 15n relaxation parameters in proteins. | a number of factors at all stages of data processing which affect the accuracy of determination of 15n relaxation parameters in 15n-labeled proteins is discussed. methods which allow to improve accuracy of the determined parameters are presented using data obtained for cucurbita maxima trypsin inhibitor. | 1999 | 10698274 |
| wound-induced ethylene synthesis and expression and formation of 1-aminocyclopropane-1-carboxylate (acc) synthase, acc oxidase, phenylalanine ammonia-lyase, and peroxidase in wounded mesocarp tissue of cucurbita maxima. | 1-aminocyclopropane-1-carboxylate (acc) synthase was rapidly induced in mesocarp tissue of cucurbita maxima after wounding in the cut surface layer in 1 mm thickness (ca. 9 cells) (first layer) in both the enzyme activity and the levels of transcript. this led to a rapid accumulation of acc and hence ethylene production. in the inside tissue (1-2 mm) (second layer), no significant induction of acc synthase was observed, which resulted in a low level of acc, although ethylene was evolved at a muc ... | 2000 | 10845457 |
| characterization of cucurbita maxima phloem serpin-1 (cmps-1). a developmentally regulated elastase inhibitor. | we report on the molecular, biochemical, and functional characterization of cucurbita maxima phloem serpin-1 (cmps-1), a novel 42-kda serine proteinase inhibitor that is developmentally regulated and has anti-elastase properties. cmps-1 was purified to near homogeneity from c. maxima (pumpkin) phloem exudate and, based on microsequence analysis, the cdna encoding cmps-1 was cloned. the association rate constant (k(a)) of phloem-purified and recombinant his(6)-tagged cmps-1 for elastase was 3.5 + ... | 2000 | 10960478 |
| electronic structure of trypsin inhibitor from squash seeds in aqueous solution. | the electronic structure of the trypsin inhibitor from seeds of the squash cucurbita maxima (cmti-i) in aqueous solution is obtained by ab initio, all-electron, full-potential calculations using the self-consistent cluster-embedding (scce) method. the reactive site of the inhibitor is explained theoretically, which is in agreement with the experimental results. it is shown that the coordinates of oxygen atoms in the inhibitor, determined by nuclear magnetic resonance and combination of distance ... | 2000 | 11089109 |
| proteolytic processing of cmpp36, a protein from the cytochrome b(5) reductase family, is required for entry into the phloem translocation pathway. | cucurbita maxima (pumpkin) phloem sap contains a 31 kda protein that cross-reacts with antibodies directed against the red clover necrotic mosaic virus movement protein (rcnmv mp). microsequence data from phloem-purified 31 kda protein were used to isolate a complementary dna: the open reading frame encodes a 36 kda protein belonging to the cytochrome b(5) reductase (cb5r) family; the gene was termed cmpp36. western analyses established that cmpp36, rcnmv mp and cmpp16 (xoconostle-cázares et al. ... | 2000 | 11135108 |
| induction of cutinolytic esterase activity during saprophytic growth of cucurbit pathogens, fusarium solani f. sp. cucurbitae races one and two (nectria haematococca mpi and mpv, respectively). | cutins from fruit of cucurbita maxima and cucurbita moschata cultivars, apple and a c(16) alcohol (hexadecanol) were used to induce cutinolytic esterase activity during saprophytic growth of strains of the two cucurbit pathogens, fusarium solani f. sp. cucurbitae, race 1 (nectria haematococca mating population (mpi) and f. solani f. sp. cucurbitae, race 2 (mpv). four strains of mpv and 11 strains of mpi were were included in the study. although we were primarily interested in the two cucurbit pa ... | 2001 | 11164297 |
| phloem-specific expression of the pumpkin fruit trypsin inhibitor. | vascular exudates of cucurbita maxima (duch.) contain a group of highly conserved serine proteinase inhibitors collectively called pumpkin fruit trypsin inhibitors (pftis) that prevent proteolytic activity of trypsin or chymotrypsin. polyclonal antibodies raised against pftis were used to immunolocalize these low-molecular-weight proteins within the phloem tissue and to study their developmental expression. the inhibitors were translocated throughout the transport phloem and were present in vasc ... | 2001 | 11216835 |
| disulfide bond effects on protein stability: designed variants of cucurbita maxima trypsin inhibitor-v. | attempts to increase protein stability by insertion of novel disulfide bonds have not always been successful. according to the two current models, cross-links enhance stability mainly through denatured state effects. we have investigated the effects of removal and addition of disulfide cross-links, protein flexibility in the vicinity of a cross-link, and disulfide loop size on the stability of cucurbita maxima trypsin inhibitor-v (cmti-v; 7 kd) by differential scanning calorimetry. cmti-v offers ... | 2001 | 11266603 |
| chromogenic substrates of bovine beta-trypsin: the influence of an amino acid residue in p1 position on their interaction with the enzyme. | the cucurbita maxima trypsin inhibitor cmti-iii molecule was used as a vehicle to design and synthesize a series of trypsin chromogenic substrates modified in position p1: ac-ala-val-abu-pro-x-pna, where x = orn, lys, arg, har, arg(no(2)), cit, hci, phe(p-cn), phe(p-nh(2)); pna = p-nitroanilide. the most active compounds (as determined by specificity constant k(cat)/k(m)) were peptides with the arg and lys residues in the position discussed. changes in the length and the decrease of the positive ... | 2001 | 11478806 |
| matrix-assisted laser desorption/ionization time of flight mass spectrometry peptide mass fingerprints and post source decay: a tool for the identification and analysis of phloem proteins from cucurbita maxima duch. separated by two-dimensional polyacrylamide gel electrophoresis. | a combination of gel electrophoresis and mass spectrometry was used to analyze the soluble proteins from phloem sap of cucurbita maxima duch. phloem proteins were separated using two-dimensional gel electrophoresis. coomassie-stained spots were cut out and subjected to tryptic digestion. to identify proteins, peptide mass fingerprints were determined by matrix-assisted laser desorption/ionization time of flight (maldi-tof) mass spectrometry. in addition, maldi-tof post source decay measurements ... | 2001 | 11556791 |
| applying science to changing dietary patterns. | the intake of 400-600 g/d of fruits and vegetables is associated with reduced incidence of many common forms of cancer. these foods contain phytochemicals that can modulate gene expression to inhibit carcinogenesis via multiple pathways. many phytochemicals are colorful, providing an easy way to communicate increased diversity of fruits and vegetables to the public. red foods contain lycopene, the pigment in tomatoes, which is localized in the prostate gland and may be involved in maintaining pr ... | 2001 | 11694651 |
| phylogenetic relationships among domesticated and wild species of cucurbita (cucurbitaceae) inferred from a mitochondrial gene: implications for crop plant evolution and areas of origin. | we have investigated the phylogenetic relationships among six wild and six domesticated taxa of cucurbita using as a marker an intron region from the mitochondrial nad1 gene. our study represents one of the first successful uses of a mtdna gene in resolving inter- and intraspecific taxonomic relationships in angiosperms and yields several important insights into the origins of domesticated cucurbita. first, our data suggest at least six independent domestication events from distinct wild ancesto ... | 2002 | 11782554 |
| analysis of highly polar compounds of plant origin: combination of hydrophilic interaction chromatography and electrospray ion trap mass spectrometry. | the primary goal of metabolomic analysis is the unbiased relative quantification of every metabolite in a biological system. a number of different metabolite-profiling techniques must be combined to make this possible. here we report the separation and analysis of highly polar compounds in a proof of concept study. compounds were separated and analyzed using hydrophilic interaction liquid chromatography (hilic) coupled to electrospray ionization (esi) mass spectrometry. two types of hilic microb ... | 2002 | 11814300 |
| analysis of the complexity of protein kinases within the phloem sieve tube system. characterization of cucurbita maxima calmodulin-like domain protein kinase 1. | in angiosperms, functional, mature sieve elements lack nuclei, vacuoles, ribosomes, and most of the endomembrane network. in this study, the complexity, number, and nature of protein kinases within the phloem sap of cucurbita maxima were investigated to test the hypothesis that the enucleate sieve tube system utilizes a simplified signal transduction network. supporting evidence was obtained in that only five putative protein kinases (three calcium-independent and two calcium-dependent protein k ... | 2002 | 11847230 |
| correlation of binding-loop internal dynamics with stability and function in potato i inhibitor family: relative contributions of arg(50) and arg(52) in cucurbita maxima trypsin inhibitor-v as studied by site-directed mutagenesis and nmr spectroscopy. | the side chains of arg(50) and arg(52) at positions p(6)' and p(8)', respectively, anchor the binding loop to the protein scaffold by means of hydrogen bonds in cucurbita maxima trypsin inhibitor-v (cmti-v), a potato i family member. here, we have investigated the relative contributions of arg(50) and arg(52) to the binding-loop flexibility and stability by determining changes in structure, dynamics, and proteolytic stability as a consequence of individually mutating them into an alanine. we hav ... | 2002 | 12135379 |
| purification and properties of ent-kaurene synthase b from immature seeds of pumpkin. | ent-kaurene synthase b (ksb) was purified 291-fold from a crude enzyme preparation from endosperm of pumpkin (cucurbita maxima l.). separation of ent-kaurene synthase a and ksb was achieved by hydrophobic interaction chromatography. the fractions containing ksb activity were further purified by diethylaminoethyl, phenyl, and hydroxyapatite column chromatography. using sodium dodecyl phosphate-polyacrylamide gel electrophoresis, the purest enzyme preparation showed a major band at an apparent mol ... | 1995 | 12228665 |
| aluminum-induced rapid root inhibition and changes in cell-wall components of squash seedlings. | growth of squash (cucurbita maxima duch.) roots was significantly inhibited by 1 mm alcl3 as early as 1 h after the treatment. the growth inhibition was confined to the elongating zone (1-6 mm from the root tip). chemical analysis of cell-wall polysaccharides from roots revealed that aluminum increased pectin, hemi-cellulose, and cellulose contents after 3 h of treatment. the effect of aluminum on pectin content was found in the elongating zone including the root tip, whereas change in cellulose ... | 1994 | 12232377 |
| a subclass of plant heat shock cognate 70 chaperones carries a motif that facilitates trafficking through plasmodesmata. | plasmodesmata establish a pathway for the trafficking of non-cell-autonomously acting proteins and ribonucleoprotein complexes. plasmodesmal enriched cell fractions and the contents of enucleate sieve elements, in the form of phloem sap, were used to isolate and characterize heat shock cognate 70 (hsc70) chaperones associated with this cell-to-cell transport pathway. three cucurbita maxima hsc70 chaperones were cloned and functional and sequence analysis led to the identification of a previously ... | 2002 | 12456884 |
| a sensitive and rapid bioassay of homogalacturonan synthase using 2-aminobenzamide-labeled oligogalacturonides. | polygalacturonate 4-alpha-galacturonosyltransferase (gala t) activity was detected in the microsomal fraction isolated from pumpkin (cucurbia moschata duchesne, cv. tokyou-kabocha) seedlings using udp-gala and 2-aminobenzamide (2ab)-labeled oligogalacturonides. a 2ab-labeled undecagalacturonide was elongated by the attachment of galacturonic acid (gala) residues to give 2ab-labeled oligogalacturonides with a degree of polymerization (dp) between 12 and 17. exogenous 2ab-labeled oligogalacturonid ... | 2002 | 12461139 |
| expression, refolding, and activation of the catalytic domain of human blood coagulation factor xii. | human blood coagulation factor xii (fxii; 80 kda) contains a c-terminal serine protease zymogen domain, which becomes activated upon contacting a negative surface. activated fxii (alphafxiia) brings about reciprocal activation of fxii and kallikrein that by further hydrolysis produces the free catalytic domain (betafxiia; 28 kda). increased levels of alphafxiia are associated with coronary heart disease, sepsis, and diabetes. biophysical investigation of the structural basis of activation, subst ... | 2003 | 12509996 |
| metabolic networks of cucurbita maxima phloem. | metabolomic analysis aims at a comprehensive characterization of biological samples. yet, biologically meaningful interpretations are often limited by the poor spatial and temporal resolution of the acquired data sets. one way to remedy this is to limit the complexity of the cell types being studied. cucurbita maxima duch. vascular exudates provide an excellent material for metabolomics in this regard. using automated mass spectral deconvolution, over 400 components have been detected in these e ... | 2003 | 12590115 |
| influence of cultivar, cooking, and storage on cell-wall polysaccharide composition of winter squash (cucurbita maxima). | changes in the cell-wall polysaccharides (cwp) of the edible tissues of four winter squash cultivars during storage and after cooking were investigated. a procedure for isolating cell walls of tissues containing high levels of starch was used. the starch-free cwp were sequentially fractionated using cdta, dilute na(2)co(3), and 4 m koh. cellulose made up 40-42% of the total cwp for three cultivars (delica, cf 2, and cf 4) at harvest but was 35% in the softer red warren. the pectic polysaccharide ... | 2003 | 12643650 |
| expression studies of gibberellin oxidases in developing pumpkin seeds. | two cdna clones, 3-ox and 2-ox, have been isolated from developing pumpkin (cucurbita maxima) embryos that show significant amino acid homology to gibberellin (ga) 3-oxidases and 2-oxidases, respectively. recombinant fusion protein of clone 3-ox converted ga(12)-aldehyde, ga(12), ga(15), ga(24), ga(25), and ga(9) to ga(14)-aldehyde, ga(14), ga(37), ga(36), ga(13), and ga(4), respectively. recombinant 2-ox protein oxidized ga(9), ga(4), and ga(1) to ga(51), ga(34), and ga(8), respectively. previo ... | 2003 | 12644672 |
| distribution of mca-coated grits in maize fields after high wheel tractor application for disrupting orientation of diabrotica virgifera virgifera leconte. | high wheel tractor applications of 4-methoxycinnamaldehyde (mca)-coated corn granules ('grits') were conducted in ruski krstur (serbia) in summer 2001 in a 5 ha corn field. grits are a by-product after corn is harvested and separated from the cob and used as a carrier medium to disseminate mca into the corn field. mca is a kairomone mimic derived form cucurbita maxima (duchesne) used to disrupt orientation of diabrotica virgifera virgifera leconte towards different mca and pheromone baited traps ... | 2002 | 12696417 |
| modulation of pumpkin glutathione s-transferases by aldehydes and related compounds. | induction of pumpkin (cucurbita maxima duch.) glutathione s-transferase (gst, ec 2.5.1.18) by aldehydes and related compounds was examined. all of the tested compounds induced pumpkin gst to different degrees, and it was found that (1) aldehydes induce gst directly and alcohols induce gst indirectly, and (2) alpha,beta-unsaturated aldehydes are the most effective inducers and their potency is related to the michael acceptors reaction. the results of western blot analysis showed that the patterns ... | 2003 | 12773634 |
| effect of blanching and frozen storage on ascorbic acid in baby blue winter squash. | 1964 | 14133711 | |
| simple and inexpensive flow l-glutamate determination using pumpkin tissue. | this work refers to a very easy to implementate flow injection system with potentiometric detection for l-glutamate determination in food samples. the proposed procedure is based on measurement of carbon dioxide produced by decarboxylation of l-glutamate catalyzed by l-glutamate decarboxylase (e.c. 4.1.1.1.5) from cucurbita maxima (pumpkin). the fi potentiometric system includes an enzymatic reactor with a length of 8 cm and thickness of 5 mm packed with 200 mg of a c. maxima outer layer cut in ... | 2003 | 14611151 |
| yield response of pumpkin and winter squash to simulated cucumber beetle (coleoptera: chrysomelidae) feeding injury. | field studies were conducted to characterize the yield responses of pumpkin, cucurbita pepo l., and wintersquash, cucurbita maxima duch., to simulated striped cucumber beetle, acalymma vittatum (f.), feeding damage. preliminary studies in pumpkin ('spookie') were conducted in 1993, and more complete studies in 1994 and 1995 in pumpkin (spookie), and in 1995 and 1996 in winter squash ('waltham'). plants were artificially injured 1 time at the cotyledon, 1st, 2nd, or 3rd true leaf stages by clippi ... | 2000 | 14658523 |
| xylem sap protein composition is conserved among different plant species. | xylem sap from broccoli (brassica oleracea l. cv. calabrais), rape (brassica napus l. cv. drakkar), pumpkin (cucurbita maxima duch. cv. gelber zentner) and cucumber (cucumis sativus l. cv. hoffmanns giganta) was collected by root pressure exudation from the surface of cut stems of healthy, adult plants. total protein concentrations were in the range of 100 microg ml(-1). one-dimensional gel electrophoresis (sds-page) resulted in 10-20 visible protein bands in a molecular mass range from 10 to 10 ... | 2004 | 15064951 |
| a systemic small rna signaling system in plants. | systemic translocation of rna exerts non-cell-autonomous control over plant development and defense. long-distance delivery of mrna has been proven, but transport of small interfering rna and microrna remains to be demonstrated. analyses performed on phloem sap collected from a range of plants identified populations of small rna species. the dynamic nature of this population was reflected in its response to growth conditions and viral infection. the authenticity of these phloem small rna molecul ... | 2004 | 15258266 |
| proteomics of curcurbit phloem exudate reveals a network of defence proteins. | many different proteins can be separated from the sap of mature sieve tubes of different plant species. to date, only a limited number of those have been identified and functionally characterised. due to sieve tubes inability of transcription and translation, the proteins are most probably synthesised in the intimately connected companion cells and transported into the sieve elements through plasmodesmata. the specific protein composition of phloem sap suggests an important role of these protein ... | 2004 | 15276438 |
| molecular and biochemical characterization of the capsicum annuum calcium-dependent protein kinase 3 (cacdpk3) gene induced by abiotic and biotic stresses. | the isolated full-length capsicum annuum calcium-dependent protein kinase 3 (cacdpk3) cdna clone was selected from the chili pepper expressed sequence tag database (http://www.pdrc.re.kr/ks200201/pepper.html). phylogenetic analysis based on the deduced amino acid sequence of cacdpk3 cdna revealed significant sequence similarity to the winter squash (cucurbita maxima) cmcpk2 gene (81% identity). genomic gel blot analysis disclosed that cacdpk3 belongs to a multigene family in the pepper genome. c ... | 2004 | 15449060 |
| localization of ascorbic acid, ascorbic acid oxidase, and glutathione in roots of cucurbita maxima l. | to understand the function of ascorbic acid (asc) in root development, the distribution of asc, asc oxidase, and glutathione (gsh) were investigated in cells and tissues of the root apex of cucubita maxima. asc was regularly distributed in the cytosol of almost all root cells, with the exception of quiescent centre (qc) cells. asc also occurred at the surface of the nuclear membrane and correspondingly in the nucleoli. no asc could be observed in vacuoles. asc oxidase was detected by immunolocal ... | 2004 | 15520029 |
| carotenemia associated with green bean ingestion. | carotenemia is a condition characterized by yellow discoloration of the skin and elevated blood carotene levels. excessive and prolonged ingestion of carotene-rich, yellow- or orange-colored foods such as carrots and winter squash is the most common cause, but more rarely it may be associated with consumption of other foods as well as with hypothyroidism, diabetes mellitus, anorexia nervosa, liver disease, or kidney disease. though not uncommon in children, there are few reports in the pediatric ... | 2016 | 15575851 |
| [preclinical studies of cucurbita maxima (pumpkin seeds) a traditional intestinal antiparasitic in rural urban areas]. | experimental research was carried out at the parasitology and chemistry laboratories of the jorge basadre grohmann national university, in tacna. the process involved two phases: (1) determination of the minimum inhibitory concentration (mic) of cucurbita maxima as an antiparasitic agent using canine tapeworms with an intestinal isolation of 5 to 6 hours, and (2) determination of the side-effects of curbita maxima on exposed albino rats. it was found that the mic of 23 gr. of pumpkin seed in 100 ... | 2004 | 15614300 |
| dietary patterns and risk of gastric cancer: a case-control study in uruguay. | gastric cancer is a frequent malignancy in the uruguayan population. in northern counties, incidence rates reach high figures (age-standardized rates [asr], 37.3 per 100,000 men and 18.3 per 100,000 women). diet is a major determinant in gastric carcinogenesis. because foods or food groups have the advantage over nutrients in being most directly related to dietary recommendations, we decided to conduct a case-control study on the relationships between food groups and risk of gastric cancer. for ... | 2004 | 15616769 |
| destination-selective long-distance movement of phloem proteins. | the phloem macromolecular transport system plays a pivotal role in plant growth and development. however, little information is available regarding whether the long-distance trafficking of macromolecules is a controlled process or passive movement. here, we demonstrate the destination-selective long-distance trafficking of phloem proteins. direct introduction, into rice (oryza sativa), of phloem proteins from pumpkin (cucurbita maxima) was used to screen for the capacity of specific proteins to ... | 2005 | 15863519 |
| squash xylem sap has activities that inhibit proliferation and promote the elongation of tobacco by-2 cell protoplasts. | to elucidate the physiological functions of the substances in xylem sap, we analyzed the biological activities of xylem sap from squash (cucurbita maxima duch.) root using tobacco by-2 (nicotiana tabacum l. cv. bright yellow 2) cell protoplasts. when by-2 cell protoplasts were cultivated with the total substance of squash xylem sap, the protoplasts elongated remarkably, and cell division was inhibited. although trans-zeatin riboside (zr), the most abundant cytokinin in squash xylem sap, had a co ... | 2005 | 15890518 |
| multi-species interactions impact the accumulation of weathered 2,2-bis (p-chlorophenyl)-1,1-dichloroethylene (p,p'-dde) from soil. | the impact of interactions between the earthworms eisenia foetida and lumbricus terrestris and the plants cucurbita pepo and cucurbita maxima on the uptake of weathered p,p'-dde from soil was determined. although some combinations of earthworm and plant species caused significant changes in the p,p'-dde burden in both organisms, the effects were species specific. contaminant bioconcentration in c. pepo was increased slightly by e. foetida and by 3-fold when the plant was grown with l. terrestris ... | 2005 | 15913856 |
| hispanic and non-hispanic white elders from massachusetts have different patterns of carotenoid intake and plasma concentrations. | carotenoids have been linked with protective roles against diseases associated with aging, including cancer, cardiovascular disease, cataracts, and age-related macular degeneration. with data from a semiquantitative, validated ffq, we examined carotenoid intake of 340 puerto ricans, 98 dominicans, and 146 non-hispanic whites (>60 y old) in massachusetts. compared with non-hispanic white men, hispanic men reported a higher intake of lycopene and lower intakes of alpha-carotene, lutein + zeaxanthi ... | 2005 | 15930459 |
| phenolic profiles of raw apricots, pumpkins, and their purees in the evaluation of apricot nectar and jam authenticity. | the possibility of proving the undeclared addition of pumpkin puree in apricot nectars and jams has been investigated by using the phenol compound fingerprint and sensory evaluation. the cheaper pumpkin admixtures in apricot nectars and jams could not be detected by the sensory evaluation, particularly if present in quantities of <15%. the lower admixtures of pumpkin puree in apricot nectars and jams could be detected by the presence of syringic acid, a phenolic compound characteristic of the in ... | 2005 | 15941324 |
| gibberellin biosynthesis in developing pumpkin seedlings. | a gibberellin (ga) biosynthetic pathway was discovered operating in root tips of 7-d-old pumpkin (cucurbita maxima) seedlings. stepwise analysis of ga metabolism in cell-free systems revealed the conversion of ga(12)-aldehyde to bioactive ga(4) and inactive ga(34). highest levels of endogenous ga(4) and ga(34) were found in hypocotyls and root tips of 3-d-old seedlings. cdna molecules encoding two ga oxidases, cmga20ox3 and cmga3ox3, were isolated from root tips of 7-d-old lab150978-treated seed ... | 2005 | 16126862 |
| cucurbit phloem serpins are graft-transmissible and appear to be resistant to turnover in the sieve element-companion cell complex. | serpins are unique inhibitors of serine proteases that are located in various plant tissues and organs. an orthologue of the pumpkin (cucurbita maxima) phloem serpin cmps-1 was amplified from cucumber (cucumis sativus) rna by rt-pcr, cloned, and designated as csps-1 (genbank accession no. aj866989). alternative amino acid sequences in the reactive centre loop suggest distinct inhibitory specificity between cmps-1 and csps-1. a difference in the electrophoretic mobility of these serpins was used ... | 2005 | 16246856 |
| the cucurbit images (1515-1518) of the villa farnesina, rome. | the gorgeous frescoes organized by the master renaissance painter raphael sanzio (1483-1520) and illustrating the heavenly adventures of cupid and psyche were painted between 1515 and 1518 to decorate the roman villa (now known as the villa farnesina) of the wealthy sienese banker agostino chigi (1466-1520). surrounding these paintings are festoons of fruits, vegetables and flowers painted by giovanni martini da udine (1487-1564), which include over 170 species of plants. a deconstruction and co ... | 2006 | 16314340 |
| ectopic expression of pumpkin gibberellin oxidases alters gibberellin biosynthesis and development of transgenic arabidopsis plants. | immature pumpkin (cucurbita maxima) seeds contain gibberellin (ga) oxidases with unique catalytic properties resulting in gas of unknown function for plant growth and development. overexpression of pumpkin ga 7-oxidase (cmga7ox) in arabidopsis (arabidopsis thaliana) resulted in seedlings with elongated roots, taller plants that flower earlier with only a little increase in bioactive ga4 levels compared to control plants. in the same way, overexpression of the pumpkin ga 3-oxidase1 (cmga3ox1) res ... | 2006 | 16384902 |
| nitrate reductase from squash: cdna cloning and nitrate regulation. | the assimilation of nitrate in plants involves the reduction of nitrate to ammonia in two steps. the first step requires nitrate reductase, a nitrate-inducible enzyme. when seedlings of squash (cucurbita maxima l.) were treated with nitrate, both nitrate reductase activity and protein were induced in the cotyledons. poly(a)(+) rna was prepared from cotyledons of nitrate-treated seedlings and was used to construct a lambdagt11 cdna library. using antibodies from mice immunized against purified ni ... | 1986 | 16593773 |
| effect of infection by hypomyces solani f. sp. cucurbitac on apparent free space, cell membrane permeability, and respiration of squash hypocotyls. | initial symptoms and increases in respiration, apparent free space, and rate of leakage of amino acids occurred concomitantly in squash (cucurbita maxima dcne) hypocotyls infected by hypomyces solani f. sp. cucurbitae snyd. and hans. young, rapidly expanding lesions had greater respiratory rates and apparent free space than comparable tissues from healthy plants.hypocotyl tissues above (1-45 mm) lesions possessed greater endogenous respiratory rates (2-3 times) and lower respiratory quotients th ... | 1968 | 16656953 |
| uptake of 3-o-methylglucose by healthy and hypomyces-infected squash hypocotyls. | rates of uptake of 3-o-methylglucose (meg) by squash (cucurbita maxima) hypocotyl sections from above lesions caused by hypomyces solani f. sp. cucurbitae, race 1, are 2-fold greater than uptake by comparable tissues from healthy plants. kinetic analyses indicate (i) that a single (constitutive) carrier system, with a michaelis constant (km) of 25 to 30 mm, mediates the transport of meg into healthy hypocotyl cells and (ii) that an additional (inducible) system with a much lower km (ca. 2 mm) is ... | 1969 | 16657201 |
| protein body composition in cuburbita maxima cotyledons as determined by energy dispersive x-ray analysis. | energy dispersive x-ray analysis was used to study the composition of certain protein body components in cucurbita maxima cotyledons. the globoid crystal was rich in phosphorus, potassium, and magnesium. this elemental composition provides further evidence that the globoid crystal in squash cotyledon protein bodies is composed of phytin, a myoinositol hexaphosphoric acid salt of potassium and magnesium. calcium, a common component of phytin in many species, was absent or present in only trace am ... | 1975 | 16659190 |
| correlation between oxygen availability, energy charge, and protein synthesis in squash cotyledons isolated from germinating seeds. | the influence of o(2) availability on the rate of protein synthesis, the levels of rna and of adenylates, and the value of the energy charge in squash (cucurbita maxima) cotyledons isolated from seeds germinated for 15 or 28 hours at different o(2) concentration (3% or 20% o(2)) has been investigated.the rate of protein synthesis is five times lower in cotyledons maintained in 3% o(2) than in those maintained in 20% o(2). also net rna synthesis is almost blocked in 3% o(2), while in 20% o(2) it ... | 1978 | 16660244 |
| energy-dispersive x-ray analysis of phosphorus, potassium, magnesium, and calcium in globoid crystals in protein bodies from different regions of cucurbita maxima embryos. | the seeds of cucurbita maxima contain protein bodies with electrondense globoid crystals. because of their density globoid crystals are ideal material for energy-dispersive x-ray (edx) analysis studies of elemental composition. fixation trials were carried out to test globoid crystal extraction during glutaraldehyde fixation, water washing, and ethanol dehydration. glutaraldehyde fixation without subsequent washing or dehydration alone produced no significant changes in elemental composition of ... | 1978 | 16660439 |
| calcium distribution in globoid crystals of cucurbita cotyledon protein bodies. | energy-dispersive x-ray analysis was used to investigate the location of globoid crystals with relatively high ca levels within cotyledons of cucurbita maxima, cucurbita mixta, and cucurbita andreana. the small globoid crystals in both upper and lower epidermal cells commonly contained ca. ca was present in globoid crystals of all provascular regions with the exception of the very small provascular regions of c. maxima. in c. maxima and c. mixta cotyledons, some cases were observed where ca was ... | 1979 | 16660825 |
| purification of squash nadh:nitrate reductase by zinc chelate affinity chromatography. | nadh:nitrate reductase (ec 1.6.6.1) was isolated and purified from the green cotyledons of 5-day-old squash seedlings (cucurbita maxima l.). the 10-hour purification procedure consisted of two steps: direct application of crude enzyme to blue sepharose and specific elution with nadh followed by direct application of this effluent to a zn(2+) column with elution by decreasing the ph of the phosphate buffer from 7.0 to 6.2. the high specific activity (100 micromoles per minute per milligram protei ... | 1983 | 16662789 |
| response of various cucurbits to infection by plasmid-harboring strains of agrobacterium. | tumor formation in cucurbit cultivars resulting from infection by various strains of agrobacterium tumefaciens and agrobacterium rhizogenes is environmentally affected. in all instances, tumors could be induced on excised cotyledons while inoculating attached cotyledons or stems resulted in no tumor formation. in addition, buttercup squash (cucurbita maxima duch. buttercup) was most susceptible to tumor formation, while butterbush squash (cucurbita maxima duch. butterbush) failed to form tumors ... | 1986 | 16665082 |
| conversion of xanthoxin to abscisic acid by cell-free preparations from bean leaves. | cell-free extracts from the leaves of phaseolus vulgaris l. convert xanthoxin to abscisic acid. the enzyme activity in dialyzed or acetone-precipitated extracts shows a strong dependence on either nad or nadp. the enzyme activity appears to be cytosolic with no significant activity observed in chloroplasts. the activity was observed in extracts from roots of phaseolus vulgaris, and also in extracts prepared from the leaves of pisum sativum l., zea mays l., cucurbita maxima duchesne, and vigna ra ... | 1987 | 16665831 |
| phytochrome-mediated light regulation of nitrate reductase expression in squash cotyledons. | in etiolated squash (cucurbita maxima l.) cotyledons, nitrate-inducible nadh:nitrate reductase activity and protein were increased in darkness by red light pulses with red/far-red photoreversibility. continuous far-red light also led to increased levels of nitrate reductase activity and protein. poly(a)+rna, which hybridizes to squash nitrate reductase cdna, was also increased by light treatments. thus, we found that after nitrate triggering, nitrate reductase expression appears to be regulated ... | 1988 | 16666287 |
| properties of a small basic peptide from pumpkin seeds. | a small basic peptide with an unusual amino acid composition has been isolated from the seeds of pumpkin, cucurbita maxima. amino acid analysis and sequence data show the protein to be about 36 residues in length, with an approximate composition lys(1), arg(14), asp(3), (glu + gln)(15), gly(1), pro(1), trp(1). on the basis of composition, the molecular weight is approximately 5000 daltons and the nitrogen content by weight is 20.4%. twelve amino acids are entirely lacking. the peptide is slightl ... | 1988 | 16666381 |
| synthesis and biological activity of an azido derivative of paclobutrazol, an inhibitor of gibberellin biosynthesis. | a photolabile azido derivative of the kaurene oxidase inhibitor 1-(4-chlorophenyl)-4,4-dimethyl-2-(1,2,4-triazol-l-yl) pentan-3-ol (paclobutrazol) has been synthesized for use as a photoaffinity labeling agent. the compound was tested as an inhibitor of the oxidation of ent-kaurene catalyzed by cell-free preparations from endosperm of cucurbita maxima. the i(50) of the azido derivative was 9.5 nanomolar, which compares well with that of paclobutrazol (6.3 nanomolar in our measurements). the azid ... | 1988 | 16666477 |
| intact plastids are required for nitrate- and light-induced accumulation of nitrate reductase activity and mrna in squash cotyledons. | induction of nitrate reductase activity and mrna by nitrate and light is prevented if chloroplasts are destroyed by photooxidation in norflurazon-treated squash (cucurbita maxima l.) cotyledons. the enzyme activity and mrna can be induced if norflurazon-treated squash seedlings are kept in low-intensity red light, which minimizes photodamage to the plastids. it is concluded that induction of nitrate reductase activity and nitrate reductase mrna requires intact plastids. if squash seedlings grown ... | 1990 | 16667294 |
| sugar composition and molecular weight distribution of cell wall polysaccharides in outer and inner tissues from segments of dark grown squash (cucurbita maxima duch.) hypocotyls. | the elongation growth of stem segments is determined by the outer cell layers (epidermis and collenchyma). we measured the sugar composition and molecular weight distribution of pectin and hemicellulose fractions obtained from inner and outer tissues of squash (cucurbita maxima duch.) hypocotyls. in addition, we studied the changes in these parameters after a 9 hour period of incubation of the segments. the results show that outer tissues have higher molecular weight pectin and hemicellulose com ... | 1990 | 16667612 |
| differential effect of auxin on molecular weight distributions of xyloglucans in cell walls of outer and inner tissues from segments of dark grown squash (cucurbita maxima duch.) hypocotyls. | effects of indole-3-acetic acid (iaa) on the mechanical properties of cell walls and structures of cell wall polysaccharides in outer and inner tissues of segments of dark grown squash (cucurbita maxima duch.) hypocotyls were investigated. iaa induced the elongation of unpeeled, intact segments, but had no effect on the elongation of peeled segments. iaa induced the cell wall loosening in outer tissues as studied by the stress-relaxation analysis but not in inner tissues. iaa-induced changes in ... | 1991 | 16668092 |
| organic substances in xylem sap delivered to above-ground organs by the roots. | squash (cucurbita maxima) xylem sap, an apoplastic fluid, contains t-zeatin riboside, glutamine, methylglycine, myo-inositol, fructose, oligosaccharides of arabinogalactan, glucan, galacturonan, and pectins (rhamnogalacturonan-i and rhamnogalacturonan-ii), as well as various proteins, including arabinogalactan and pathogen-related proteins. these substances are mainly produced in stele (xylem) parenchyma and the pericycle in the root-hair zone where ion transporter genes are expressed. glycine-r ... | 2006 | 16733632 |
| a putative acyl-coa-binding protein is a major phloem sap protein in rice (oryza sativa l.). | the n-terminal amino-acid sequence of a major rice phloem-sap protein, named rpp10, was determined. rpp10 is encoded by a single gene in the rice genome. its complete amino-acid sequence, predicted from the corresponding rice full-length cdna, showed high similarity to plant acyl-coa-binding proteins (acbps). western blot analysis using anti-acbp antiserum revealed that putative acbp is abundant in the phloem sap of rice plants, and is also present in sieve-tube exudates of winter squash (cucurb ... | 2006 | 16804052 |
| the squash aspartic proteinase inhibitor sqapi is widely present in the cucurbitales, comprises a small multigene family, and is a member of the phytocystatin family. | the squash (cucurbita maxima) phloem exudate-expressed aspartic proteinase inhibitor (sqapi) is a novel aspartic acid proteinase inhibitor, constituting a fifth family of aspartic proteinase inhibitors. however, a comparison of the sqapi sequence to the phytocystatin (a cysteine proteinase inhibitor) family sequences showed approximately 30% identity. modeling sqapi onto the structure of oryzacystatin gave an excellent fit; regions identified as proteinase binding loops in cystatin coincided wit ... | 2006 | 17103059 |
| kinetic and thermodynamic investigation on ascorbate oxidase activity and stability of a cucurbita maxima extract. | the kinetic and thermodynamic properties of ascorbate oxidase (ao) activity and stability of a cucurbita maxima extract were investigated. activity tests performed at 25 degrees c using initial ascorbic acid concentration in the range 50-750 m allowed estimating the michaelis constant for this substrate (km = 126 microm) and the maximum initial rate of ascorbic acid oxidation (a0,max = 1.57 mm min-1). the main thermodynamic parameters of the enzyme reaction (deltah* = 10.3 kj mol-1; deltag* = 87 ... | 2006 | 17137312 |
| differences in p,p'-dde bioaccumulation from compost and soil by the plants cucurbita pepo and cucurbita maxima and the earthworms eisenia fetida and lumbricus terrestris. | two plant species, cucurbita pepo and cucurbita maxima, and two earthworm species, eisenia fetida and lumbricus terrestris, were exposed to soil and compost with equivalent p,p'-dde contamination. pollutant bioconcentration was equal in plant roots in both media, but translocation was higher in c. pepo. bioaccumulation by e. fetida was approximately 6- and 3-fold higher than that by l. terrestris in the soil and compost, respectively. for all species, p,p'-dde uptake was significantly greater fr ... | 2007 | 17241722 |
| allozyme segregation ratios in the interspecific cross cucurbita maximaxc. ecuadorensis suggest that hybrid breakdown is not caused by minor alterations in chromosome structure. | the parentals of the interspecific cross cucurbita maxima xc. ecuadorensis had different isozyme phenotypes for 12 enzyme systems. characterization of the systems demonstrated that the expression and intracellular distribution of the isozymes were similar to those in other plant taxa; however, a considerable number of duplicate loci were identified, indicative of a polyploid ancestry for cucurbita. genetic analysis provided evidence for 20 loci segregating in f(2) and backcross populations. five ... | 1986 | 17246350 |
| ethnomedicines used in trinidad and tobago for reproductive problems. | throughout history women have tried to control or enhance their fertility using herbal remedies, with various levels of societal support. caribbean folk medicine has been influenced by european folk medicine, either through the early spanish and french settlers or through the continuous immigration of spanish-speaking peoples from venezuela. some folk uses are ancient and were documented by galen and pliny the elder. | 2007 | 17362507 |
| biosynthesis of sterols and triterpenoids in tissue cultures of cucurbita maxima. | the biosynthesis of sterols and triterpenoids in cucurbita maxima was studied by analysis of unsaponifiable fraction of tissues from different development stages of the plant (seeds, seedlings, adult plant and tissue culture) and by feeding germinating seeds and tissue cultures with [2- (14)c]-acetate. synthesis of cucurbitacins does not occur in callus tissues of cucurbita maxima, whereas a wide variety of 4,4-dimethylsterols present in these tissues testifies of a high level of squaleneoxide c ... | 1983 | 17405044 |
| oil and tocopherol content and composition of pumpkin seed oil in 12 cultivars. | twelve pumpkin cultivars (cucurbita maxima d.), cultivated in iowa, were studied for their seed oil content, fatty acid composition, and tocopherol content. oil content ranged from 10.9 to 30.9%. total unsaturated fatty acid content ranged from 73.1 to 80.5%. the predominant fatty acids present were linoleic, oleic, palmitic, and stearic. significant differences were observed among the cultivars for stearic, oleic, linoleic, and gadoleic acid content of oil. low linolenic acid levels were observ ... | 2007 | 17439238 |
| qualitative and quantitative differences in carotenoid composition among cucurbita moschata, cucurbita maxima, and cucurbita pepo. | squashes and pumpkins are important dietary sources of carotenoids worldwide. the carotenoid composition has been determined, but reported data have been highly variable, both qualitatively and quantitatively. in the present work, the carotenoid composition of squashes and pumpkins currently marketed in campinas, brazil, were determined by hplc-dad, complemented by hplc-ms for identification. cucurbita moschata 'menina brasileira' and c. moschata 'goianinha' had similar profiles, with beta-carot ... | 2007 | 17444652 |
| flowering locus t protein may act as the long-distance florigenic signal in the cucurbits. | cucurbita moschata, a cucurbit species responsive to inductive short-day (sd) photoperiods, and zucchini yellow mosaic virus (zymv) were used to test whether long-distance movement of flowering locus t (ft) mrna or ft is required for floral induction. ectopic expression of ft by zymv was highly effective in mediating floral induction of long-day (ld)-treated plants. moreover, the infection zone of zymv was far removed from floral meristems, suggesting that ft transcripts do not function as the f ... | 2007 | 17540715 |
| larvicidal, ovicidal and repellent activities of the leaf extract of two cucurbitacious plants against filarial vector culex quinquefasciatus (say) (diptera : culicidae). | the leaf extract of two cucurbitacious plants citrullus colocynthis and cucurbita maxima with different solvents viz., benzene, ethylacetate, petroleum ether and methanol were tested for larvicidal, ovicidal and repellent activities against the mosquito culex quinquefasciatus. larval mortality was observed and recorded after 24 h exposure period. the lc50 values of c. colocynthis were 61.72, 47.58, 66.92 and 118.74 ppm respectively. c. maxima shows the lc50 values of 123.02, 75.91, 117.73 and 17 ... | 2007 | 17568371 |
| reciprocal phosphorylation and glycosylation recognition motifs control ncapp1 interaction with pumpkin phloem proteins and their cell-to-cell movement. | in plants, cell-to-cell trafficking of non-cell-autonomous proteins (ncaps) involves protein-protein interactions, and a role for posttranslational modification has been implicated. in this study, proteins contained in pumpkin (cucurbita maxima cv big max) phloem sap were used as a source of ncaps to further explore the molecular basis for selective ncap trafficking. protein overlay assays and coimmunoprecipitation experiments established that phosphorylation and glycosylation, on both nicotiana ... | 2007 | 17601822 |
| [effects of nacl stress on polyamine contents in grafted cucumber leaves]. | with a japanese salt-tolerant pumpkin cultivar 'king shintosa' (cucurbita maxima x c. moschata) as rootstock and a cucumber (cucumis sativus) cultivar 'xintaimici' as cion, this paper studied the temporal dynamics of different form polyamines contents in the leaves of grafted and own-rooted cucumber plants under 100 mmol x l(-1) nacl stress. the results showed that the free putrescence (put) content of graftedplant leaves (g2) was significantly higher than that of own-rooted plant leaves (o2), e ... | 2007 | 17615880 |
| isolation and expression of ga 2-oxidase2 in tomato. | ga 2-oxidases, a key enzyme involves ga biosynthesis, catalyze the degradation of active c(19)-gibberellins (gas) through 2-hydroxylation yields inactive ga product. searching public tomato database, the putative ga2ox2 sequences were assembled. we isolated a full-length ga2ox2 cdna with primers designed from the assembled sequence. this gene was designed as slga2ox2 (genbank accession no. ef017805). the full-length ga2ox2 gene contained a complete open reading frame (orf) of 1203 bp, which enco ... | 2007 | 17676476 |
| bacterial leafspot on hubbard squash. | 1926 | 17735959 | |
| engineered resistance against proteinases. | exogenous proteinase inhibitors are valuable and economically interesting protective biotechnological tools. we examined whether small proteinase inhibitors when fused to a selected target protein can protect the target from proteolytic degradation without simultaneously affecting the function and activity of the target domain. two proteinase inhibitors were studied: a kazal-type silk proteinase inhibitor (spi2) from galleria mellonella, and the cucurbita maxima trypsin inhibitor i (cmti i). bot ... | 2007 | 17823663 |
| content and in-vitro accessibility of pro-vitamin a carotenoids from sri lankan cooked non-leafy vegetables and their estimated contribution to vitamin a requirement. | vitamin a deficiency is a public health problem in sri lanka, which affects especially pre-school children. carrots (daucus carota), pumpkins (cucurbita maxima), squashes (cucurbita moschata) and sweet potatoes (ipomoea batatas) of orange, yellow-fleshed varieties are good sources of provitamin a carotenoids, but have not been studied in sri lanka in terms of the food as eaten. the content of carotenoids in each preparation method and the in-vitro accessibility of beta-carotene and alpha-caroten ... | 2007 | 17852483 |
| nutritional and anti-nutritional compositions of cleome rutidosperma, lagenaria siceraria, and cucurbita maxima seeds from nigeria. | flours produced from the seeds of cleome rutidosperma (dc), lagenaria siceraria (mol.), and cucurbita maxima (duch) were evaluated for proximate and elemental compositions. preliminary phytochemical screening was also carried out on the seed flours, which revealed the presence of alkaloids, steroids, pentose, and reducing sugars in all the seed types. tannins, flavonoids, and cardiac glycosides were also detected in only c. rutidosperma seed. the results also showed that the seeds of c. rutidosp ... | 2007 | 18158851 |
| uptake, translocation, and accumulation of manufactured iron oxide nanoparticles by pumpkin plants. | rapid development and application of nanomaterials and nanotechnology make assessment of their potential health and environmental impacts on humans, non-human biota, and ecosystems imperative. here we show that pumpkin plants (cucurbita maxima), grown in an aqueous medium containing magnetite (fe3o4) nanoparticles, can absorb, translocate, and accumulate the particles in the plant tissues. these results suggest that plants, as an important component of the environmental and ecological systems, n ... | 2008 | 18528537 |
| pancreatic proteolytic enzymes of ostrich purified on immobilized protein inhibitors. characterization of a new form of chymotrypsin (chtr1). | four forms of chymotrypsin (chtr1, chtr2, chtr3, chtr4), one form of trypsin and one form of elastase were purified from a slightly alkaline extract of ostrich (struthio camelus) pancreas. the zymogens in the crude extract were activated with immobilized trypsin and then separated by affinity chromatography using immobilized inhibitors and ion exchange chromatography. one of the purified forms of chymotrypsin (chtr1) exhibited an unusual interaction with the highly selective protein trypsin inhi ... | 2008 | 18598777 |
| influence of light during storage on composition of blue hubbard squash. | 2016 | 18878042 | |
| melon resistance to cucurbit yellow stunting disorder virus is characterized by reduced virus accumulation. | abstract the pattern of accumulation of cucurbit yellow stunting disorder virus (cysdv; genus crinivirus, family closteroviridae) rna has been analyzed in several cucurbit accessions. in susceptible accessions of melon (cucumis melo), cucumber (cucumis sativus), marrow (cucurbita maxima), and squash (cucurbita pepo), cysdv rna accumulation peaked during the first to second week postinoculation in the first to third leaf above the inoculated one; younger leaves showed very low or undetectable lev ... | 2003 | 18943165 |
| evaluation of alternatives to methyl bromide in melon crops in guatemala. | the monoculture of melon in guatemala has caused the massive appearance of plants with an analogous syndrome for the well-known disease commonly called melon collapse, or vine decline, causing significant losses in crops. methyl bromide is commonly used to sterilize soil prior to planting in guatemala, but it must be phased out by 2015. the objective of this study was to evaluate the technique of grafting melon onto hybrids of cucurbita (cucurbita maxima x cucurbita moschata), as an alternative ... | 2009 | 18998189 |
| a polypyrimidine tract binding protein, pumpkin rbp50, forms the basis of a phloem-mobile ribonucleoprotein complex. | rna binding proteins (rbps) are integral components of ribonucleoprotein (rnp) complexes and play a central role in rna processing. in plants, some rbps function in a non-cell-autonomous manner. the angiosperm phloem translocation stream contains a unique population of rbps, but little is known regarding the nature of the proteins and mrna species that constitute phloem-mobile rnp complexes. here, we identified and characterized a 50-kd pumpkin (cucurbita maxima cv big max) phloem rna binding pr ... | 2009 | 19122103 |
| the phloem-delivered rna pool contains small noncoding rnas and interferes with translation. | in plants, the vascular tissue contains the enucleated sieve tubes facilitating long-distance transport of nutrients, hormones, and proteins. in addition, several mrnas and small interfering rnas/micrornas were shown to be delivered via sieve tubes whose content is embodied by the phloem sap (ps). a number of these phloem transcripts are transported from source to sink tissues and function at targeted tissues. to gain additional insights into phloem-delivered rnas and their potential role in sig ... | 2009 | 19261735 |
| relationship between crop losses and initial population densities of meloidogyne arenaria in winter-grown oriental melon in korea. | to determine the economic threshold level, oriental melon (cucumis melo l. cv. geumssaragi-euncheon) grafted on shintozoa (cucurbita maxima x cu. moschata) was planted in plots (2 x 3 m) under a plastic film in february with a range of initial population densities (pi) of meloidogyne arenaria. the relationships of early, late, and total yield to pi measured in september and january were adequately described by both linear regression and the seinhorst damage model. initial nematode densities in s ... | 2002 | 19265907 |
| ecology of nematodes under influence of cucurbita spp. and different fertilizer types. | in a field study conducted in georgia, cucurbit plants with high (cucurbita andreana) and low (cucurbita maxima) concentrations of cucurbitacins were used in combination with two types of fertilizers to investigate their effects on the community of soil nematodes. ecological measures of soil nematode community structure such as total nematode abundance, number of genera, trophic diversity, trophic group proportions, fungivore/bacterivore ratio, and modified maturity index were assessed and compa ... | 1995 | 19277330 |
| exact and effective pair-wise potential for protein-ligand interactions obtained from a semiempirical energy partition. | in this work, the partition method introduced by carvalho and melo was used to study the complex between cucurbita maxima trypsin inhibitor (cmti-i) and glycerol at the am1 level. an effective potential, combining non-bonding and polarization plus charge transfer (plct) terms, was introduced to evaluate the magnitude of the interaction between each amino acid and the ligand. in this case study, the nonbonding-plct non-compensation characterizes the stabilization energy of the association process ... | 2008 | 19325823 |
| extraction of ascorbate oxidase from cucurbita maxima by continuous process in perforated rotating disc contactor using aqueous two-phase systems. | the ascorbate oxidase is the enzyme used to determine the content of ascorbic acid in the pharmaceutical and food industries and clinics analyses. the techniques currently used for the purification of this enzyme raise its production cost. thus, the development of alternative processes and with the potential to reduce costs is interesting. the application of aqueous two-phase system is proposed as an alternative to purification because it enables good separation of biomolecules. the objective of ... | 2010 | 19412579 |
| antioxidative activity of water soluble polysaccharide in pumpkin fruits (cucurbita maxima duchesne). | we evaluated the antioxidative activity of a water soluble polysaccharide fraction (wsp) from pumpkin fruits (cucurbita maxima duchesne). in the wsp, dpph radical scavenging and superoxide dismutase-like activity increased depending on the total sugar content. furthermore, the wsp can serve as an inhibitor of ascorbic acid oxidation. the efficacy was also affected by the total sugar content. | 2009 | 19502750 |
| using trap crops for control of acalymma vittatum (coleoptera: chrysomelidae) reduces insecticide use in butternut squash. | striped cucumber beetle, acalymma vittatum f., is the primary insect pest of cucurbit crops in the northeastern united states. adult beetles colonize squash crops from field borders, causing feeding damage at the seedling stage and transmitting bacterial wilt erwinia tracheiphila hauben et al. 1999. conventional control methods rely on insecticide applications to the entire field, but surrounding main crops with a more attractive perimeter could reduce reliance on insecticides. a. cittatum shows ... | 2009 | 19610425 |
| tryptophan exposure and accessibility in the chitooligosaccharide-specific phloem exudate lectin from pumpkin (cucurbita maxima). a fluorescence study. | the exposure and accessibility of the tryptophan residues in the chitooligosaccharide-specific pumpkin (cucurbita maxima) phloem exudate lectin (ppl) have been investigated by fluorescence spectroscopy. the emission lambda(max) of native ppl, seen at 338nm was red-shifted to 348nm upon denaturation by 6m gdn.hcl in the presence of 10mm beta-mercaptoethanol, indicating near complete exposure of the tryptophan residues to the aqueous medium, whereas a blue-shift to 335nm was observed in the presen ... | 2009 | 19700341 |
| allergy from giant pumpkin (cucurbita maxima) is not a fairy tale. | 2009 | 19732049 | |
| rapid affinity-purification and physicochemical characterization of pumpkin (cucurbita maxima) phloem exudate lectin. | the chito-oligosaccharide-specific lectin from pumpkin (cucurbita maxima) phloem exudate has been purified to homogeneity by affinity chromatography on chitin. after sds/page in the presence of 2-mercaptoethanol, the pumpkin phloem lectin yielded a single band corresponding to a molecular mass of 23.7 kda, whereas esi-ms (electrospray ionization ms) gave the molecular masses of the subunit as 24645 da. analysis of the cd spectrum of the protein indicated that the secondary structure of the lecti ... | 2010 | 19735229 |
| comparison of perimeter trap crop varieties: effects on herbivory, pollination, and yield in butternut squash. | perimeter trap cropping (ptc) is a method of integrated pest management (ipm) in which the main crop is surrounded with a perimeter trap crop that is more attractive to pests. blue hubbard (cucurbita maxima duch.) is a highly effective trap crop for butternut squash (c. moschata duch. ex poir) attacked by striped cucumber beetles (acalymma vittatum fabricius), but its limited marketability may reduce adoption of ptc by growers. research comparing border crop varieties is necessary to provide opt ... | 2009 | 19791616 |
| differential responses in yield of pumpkin (cucurbita maxima l.) and nightshade (solanum retroflexum dun.) to the application of three animal manures. | crop responses to different manures differs considerably, however, the factors responsible for it have not been conclusively elucidated. consequently, this study examined the biomass response of cucurbita maxima and solanum retroflexum to application rates of chicken and kraal manures of cattle and goat, and soil factors related to salinity. the crops' biomass yield increased linearly with increase in application rates of kraal and chicken manures, but steeper in the latter. results showed that ... | 2010 | 19954967 |
| coccinellids, aphids, and pollen in diversified vegetable fields with transgenic and isoline cultivars. | the influence of concurrent introduction of three transgenic vegetable cultivars on seasonal dynamics of coccinellids and their food, aphids and pollen, was examined within diversified farm systems practicing insect pest management in northeastern us agroecosystems. the transgenic cultivars used included sweet corn, potato, and winter squash, expressing cry1(a)b, cry3a, and plant viral coat proteins that target lepidoptera, coleoptera, and aphid-transmitted viruses, respectively. transgenic syst ... | 2007 | 20307238 |