| carotenoid content and in vitro bioaccessibility of lutein in some leafy vegetables popular in sri lanka. | lutein is one of the major carotenoids in green leafy vegetables (glvs), which show a marked antioxidant activity. the study was carried out to identify and quantify the carotenoid content of selected sri lankan glvs. rp-hplc with photodiode array detection method was followed for the separation, identification and quantification of carotenoids. among the vegetables analyzed, ipomoea batatas leaves showed the highest beta-carotene content (743.9+/-35.0 microg/g dry weight (dw)) while syngonium a ... | 2010 | 20651462 |
| larvicidal, ovicidal and repellent activities of the leaf extract of two cucurbitacious plants against filarial vector culex quinquefasciatus (say) (diptera : culicidae). | the leaf extract of two cucurbitacious plants citrullus colocynthis and cucurbita maxima with different solvents viz., benzene, ethylacetate, petroleum ether and methanol were tested for larvicidal, ovicidal and repellent activities against the mosquito culex quinquefasciatus. larval mortality was observed and recorded after 24 h exposure period. the lc50 values of c. colocynthis were 61.72, 47.58, 66.92 and 118.74 ppm respectively. c. maxima shows the lc50 values of 123.02, 75.91, 117.73 and 17 ... | 2007 | 17568371 |
| proteomics and metabolomics analyses reveal the cucurbit sieve tube system as a complex metabolic space. | the plant vascular system, and specifically the phloem, plays a pivotal role in allocation of fixed carbon to developing sink organs. although the processes involved in loading and unloading of sugars and amino acids are well characterized, little information is available regarding the nature of other metabolites in the sieve tube system (sts) at specific sites along the pathway. here, we elucidate spatial features of metabolite composition mapped with phloem enzymes along the cucurbit sts. phlo ... | 2016 | 27155400 |
| accumulation of weathered p,p'-ddts in grafted watermelon. | the grafting of melon plants onto cucurbit rootstocks is a common commercial practice in many parts of the world. however, certain cucurbits have been shown to accumulate large quantities of weathered persistent organic pollutants from the soil, and the potential contamination of grafted produce has not been thoroughly evaluated. large pot and field experiments were conducted to assess the effect of grafting on accumulation of weathered ddx (the sum of p,p'-ddt, p,p'-ddd, and p,p'-dde) from soil ... | 2012 | 22224752 |
| the cucurbit images (1515-1518) of the villa farnesina, rome. | the gorgeous frescoes organized by the master renaissance painter raphael sanzio (1483-1520) and illustrating the heavenly adventures of cupid and psyche were painted between 1515 and 1518 to decorate the roman villa (now known as the villa farnesina) of the wealthy sienese banker agostino chigi (1466-1520). surrounding these paintings are festoons of fruits, vegetables and flowers painted by giovanni martini da udine (1487-1564), which include over 170 species of plants. a deconstruction and co ... | 2006 | 16314340 |
| sodium and chloride exclusion and retention by non-grafted and grafted melon and cucurbita plants. | the effects of grafting on na and cl(-) uptake and distribution in plant tissues were quantified in a greenhouse experiment using six combinations of melon (cucumis melo l. cv. arava) and pumpkin (cucurbita maxima duchesne×cucurbita moschata duchesne cv. tz-148): non-grafted, self-grafted, melons grafted on pumpkins, and pumpkins grafted on melons. total na concentration in shoots of plants with pumpkin or melon rootstocks was <60 mmol kg(-1) and >400 mmol kg(-1), respectively, regardless of the ... | 2011 | 20729482 |
| a comparative analysis of phloem exudate proteins from cucumis melo, cucumis sativus and cucurbita maxima by polyacrylamide gel electrophoresis and isoelectric focusing. | proteins in sieve tube exudate from cucumis melo l., cucumis sativus l. and cucurbita maxima duch. were analysed by gel electrophoresis and isoelectric focusing. estimated molecular weights and isoelectric points for the major and minor proteins from each plant species are presented. electrophoresis revealed striking differences between the protein complements of exudatc from the two genera investigated. similarly, although a few exudate proteins from the two species of cucumis possessed identic ... | 1976 | 24424601 |
| can adverse effects of acidity and aluminum toxicity be alleviated by appropriate rootstock selection in cucumber? | low-ph and aluminum (al) stresses are the major constraints that limit crop yield in acidic soils. grafting vegetable elite cultivars onto appropriate rootstocks may represent an effective tool to improve crop tolerance to acidity and al toxicity. two greenhouse hydroponic experiments were performed to evaluate growth, yield, biomass production, chlorophyll index, electrolyte leakage, mineral composition, and assimilate partitioning in plant tissues of cucumber plants (cucumis sativus l. "ekron" ... | 2016 | 27621740 |
| comparative proteomics of cucurbit phloem indicates both unique and shared sets of proteins. | cucurbits are well-studied models for phloem biology but unusually possess both fascicular phloem (fp) within vascular bundles and additional extrafascicular phloem (efp). although the functional differences between the two systems are not yet clear, sugar analysis and limited protein profiling have established that fp and efp have divergent compositions. here we report a detailed comparative proteomics study of fp and efp in two cucurbits, pumpkin and cucumber. we re-examined the sites of exuda ... | 2016 | 27472661 |
| cucumber (cucumis sativus l.) and kabocha squash (cucurbita moschata duch). | we established improved methods for agrobacterium-mediated transformation of cucumber (cucumis sativus l.) and kabocha squash (cucurbita moschata duch). vacuum infiltration of cotyledonary explants with agrobacterium suspension enhanced the agrobacterium infection efficiency in the proximal regions of explants. wounding treatment was also essential for kabocha squash. cocultivation on filter paper wicks suppressed necrosis of explants, keeping regeneration efficacy. putative transgenic plants we ... | 2015 | 25300850 |
| [salt stress tolerance of cucumber-grafted rootstocks]. | taking 4 different cucurbita maxima x c. moschata rootstocks for cucumber (cucumis sativus) as test materials, a solution culture experiment was conducted to study their growth and antioxidative enzyme activities under the stresses of ca(no3)2 and nacl, with the salt stress tolerance of the rootstocks evaluated by subordinate function. at 30 mmol x l(-1) of ca (no3)2 or 45 mmol x l(-1) of nacl, the growth of the rootstock seedlings was improved; but at 60 and 120 mmol x l(-1) of ca(no3)2 or 90 a ... | 2012 | 22919842 |
| the origin and composition of cucurbit "phloem" exudate. | cucurbits exude profusely when stems or petioles are cut. we conducted studies on pumpkin (cucurbita maxima) and cucumber (cucumis sativus) to determine the origin and composition of the exudate. morphometric analysis indicated that the exudate is too voluminous to derive exclusively from the phloem. cold, which inhibits phloem transport, did not interfere with exudation. however, ice water applied to the roots, which reduces root pressure, rapidly diminished exudation rate. sap was seen by micr ... | 2012 | 22331409 |
| vascular expression in arabidopsis is predicted by the frequency of ct/ga-rich repeats in gene promoters. | phloem-transported signals play an important role in regulating plant development and in orchestrating responses to environmental stimuli. among such signals, phloem-mobile rnas have been shown to play an important role as long-distance signaling agents. at maturity, angiosperm sieve elements are enucleate, and thus transcripts in the phloem translocation stream probably originate from the nucleate companion cells. in the present study, a pumpkin (cucurbita maxima) phloem transcriptome was used ... | 2011 | 21435051 |
| [effects of nacl stress on polyamine contents in grafted cucumber leaves]. | with a japanese salt-tolerant pumpkin cultivar 'king shintosa' (cucurbita maxima x c. moschata) as rootstock and a cucumber (cucumis sativus) cultivar 'xintaimici' as cion, this paper studied the temporal dynamics of different form polyamines contents in the leaves of grafted and own-rooted cucumber plants under 100 mmol x l(-1) nacl stress. the results showed that the free putrescence (put) content of graftedplant leaves (g2) was significantly higher than that of own-rooted plant leaves (o2), e ... | 2007 | 17615880 |
| organic substances in xylem sap delivered to above-ground organs by the roots. | squash (cucurbita maxima) xylem sap, an apoplastic fluid, contains t-zeatin riboside, glutamine, methylglycine, myo-inositol, fructose, oligosaccharides of arabinogalactan, glucan, galacturonan, and pectins (rhamnogalacturonan-i and rhamnogalacturonan-ii), as well as various proteins, including arabinogalactan and pathogen-related proteins. these substances are mainly produced in stele (xylem) parenchyma and the pericycle in the root-hair zone where ion transporter genes are expressed. glycine-r ... | 2006 | 16733632 |
| cucurbit phloem serpins are graft-transmissible and appear to be resistant to turnover in the sieve element-companion cell complex. | serpins are unique inhibitors of serine proteases that are located in various plant tissues and organs. an orthologue of the pumpkin (cucurbita maxima) phloem serpin cmps-1 was amplified from cucumber (cucumis sativus) rna by rt-pcr, cloned, and designated as csps-1 (genbank accession no. aj866989). alternative amino acid sequences in the reactive centre loop suggest distinct inhibitory specificity between cmps-1 and csps-1. a difference in the electrophoretic mobility of these serpins was used ... | 2005 | 16246856 |
| proteomics of curcurbit phloem exudate reveals a network of defence proteins. | many different proteins can be separated from the sap of mature sieve tubes of different plant species. to date, only a limited number of those have been identified and functionally characterised. due to sieve tubes inability of transcription and translation, the proteins are most probably synthesised in the intimately connected companion cells and transported into the sieve elements through plasmodesmata. the specific protein composition of phloem sap suggests an important role of these protein ... | 2004 | 15276438 |
| a systemic small rna signaling system in plants. | systemic translocation of rna exerts non-cell-autonomous control over plant development and defense. long-distance delivery of mrna has been proven, but transport of small interfering rna and microrna remains to be demonstrated. analyses performed on phloem sap collected from a range of plants identified populations of small rna species. the dynamic nature of this population was reflected in its response to growth conditions and viral infection. the authenticity of these phloem small rna molecul ... | 2004 | 15258266 |
| translocation of structural p proteins in the phloem. | phloem-specific proteins (p proteins) are particularly useful markers to investigate long-distance trafficking of macromolecules in plants. in this study, genus-specific molecular probes were used in combination with intergeneric grafts to reveal the presence of a pool of translocatable p protein subunits. immunoblot analyses demonstrated that cucurbita spp p proteins pp1 and pp2 are translocated from cucurbita maxima stocks and accumulate in cucumis sativus scions. cucurbita maxima or cucurbita ... | 1999 | 9878637 |
| effects of 3-methylgibberellin analogs on gibberellin 3β-hydroxylases and plant growth. | six 3-methylgibberellin analogs were synthesized, and their effects on the ga 3β-hydroxylases from immature seeds of phaseolus vulgaris and cucurbita maxima, and/or on the growth of dwarf rice (oryza sativa l. cv. tan-ginbozu) and cucumber (cucumis sativus l. cv. spacemaster) were investigated. 3-methyl-ga5 and 2, 3-didehydro-3-methyl-ga9· inhibited the conversion of [2, 3-(3)h2]ga9 to [2-(3)h]ga4 by ga 3β-hydroxylases from both p. vulgaris and c. maxima at 3 μm and higher. their c/d-ring-rearra ... | 1992 | 27286375 |
| effects of 60-hz electric fields on cellular elongation and radial expansion growth in cucurbit roots. | serial longitudinal and transverse sections were prepared from roots of cucumis sativus and cucurbita maxima that had been exposed/sham-exposed to 60-hz electric fields for 0-2 days. field exposures were selected to produce a 10-20% or a 70-80% growth inhibition in whole roots of both species. cortical cell length and diameter were measured using a microscope and eyepiece micrometer; measurements were conducted "blind." in both species, inhibition of cellular elongation was associated with expos ... | 1987 | 3580000 |
| sterols of cucurbitaceae: the configurations at c-24 of 24-alkyl-δ(5)-,δ(7)- and δ(8)-sterols. | the major sterols of the seeds ofbenincasa cerifera, cucumis sativus, cucurbita maxima, c. pepo andtrichosanthes japonica and of the mature plant tissues (leaves and stems) ofcitrullus battich, cucumis sativus andgynostemma pentaphyllum of the family cucurbitaceae were 24-ethyl-δ(7)-sterols which were accompanied by small amounts of saturated and δ(5)-and δ(8)-sterols. the 24-ethyl-δ(7,22),δ(7,25(27)) and δ(7,22,25(27))-sterols constituted the predominant sterols for the seed materials, whereas ... | 1986 | 27519240 |
| induction of elf transmembrane potentials in relation to power-frequency electric field bioeffects in a plant root model system. ii. the effect of 60 hz electric fields on the growth of different regions of the cucurbit root elongation zone. | the region of elongation in cucumis sativus and cucurbita maxima roots was marked at increasing distances from the apex to provide an analog of increasing cell size. these roots were exposed/sham-exposed to 60 hz electric fields and the growth rates of the root segments measured. the growth rate effect magnitude varied with increasing distance from the root tip at constant field strength, and with increasing applied field strength. these results provide strong, qualitative support for the postul ... | 1986 | 3763828 |
| induction of elf transmembrane potentials in relation to power-frequency electric field bioeffects in a plant root model system. i. relationship between applied field strength and cucurbitaceous root growth rates. | seminal roots of cucumis sativus and cucurbita maxima were exposed to 60 hz electric fields of 100-500 v x m-1 in a conducting aqueous inorganic growth medium. root growth rates were measured to produce a dose-response relationship for each species. the species were selected for study because of their familial relationship, reported sensitivity to 60 hz, 360 v x m-1 electric fields, and differing average root cell sizes. the latter characteristic influences the magnitude of elf membrane potentia ... | 1986 | 3763827 |
| phylogenetic relationships among domesticated and wild species of cucurbita (cucurbitaceae) inferred from a mitochondrial gene: implications for crop plant evolution and areas of origin. | we have investigated the phylogenetic relationships among six wild and six domesticated taxa of cucurbita using as a marker an intron region from the mitochondrial nad1 gene. our study represents one of the first successful uses of a mtdna gene in resolving inter- and intraspecific taxonomic relationships in angiosperms and yields several important insights into the origins of domesticated cucurbita. first, our data suggest at least six independent domestication events from distinct wild ancesto ... | 2002 | 11782554 |
| ribosome inactivating protein and lectin from bitter melon (momordica charantia) seeds: sequence comparison with related proteins. | one of the ribosome inactivating proteins (rips), beta-momorcharin, and a lectin were isolated from seeds of the bitter melon momordica charantia (family cucurbitaceae) in accordance with published procedures. both beta-momorcharin and m. charantia lectin were then subjected to amino acid sequencing. beta-momorcharin exhibited considerable homology in sequence to other cucurbitaceae rips, and also to the a chains of abrin and ricin which are type ii (double-chained) rips. the resemblance between ... | 1998 | 9875234 |
| dechlorination and chlorine rearrangement of 1,2,5,5,6,9,10-heptachlorodecane mediated by the whole pumpkin seedlings. | short chain chlorinated paraffins (sccps) are ubiquitously present as persistent organic pollutants in the environment. however, little information on the interaction of sccps with plants is currently available. in this work, young pumpkin plants (cucurbita maxima × c. moschata) were hydroponically exposed to the congener of chlorinated decane, 1,2,5,5,6,9,10-heptachlorodecane (1,2,5,5,6,9,10-hepcd), to investigate the uptake, translocation and transformation of chlorinated decanes in the intact ... | 2017 | 28242253 |
| no accumulation alleviates h2 o2 -dependent oxidative damage induced by ca(no3 )2 stress in the leaves of pumpkin-grafted cucumber seedlings. | nitric oxide (no) and hydrogen peroxide (h2 o2 ), two important signaling molecules, are stimulated in plants by abiotic stresses. in this study, we investigated the role of no and its interplay with h2 o2 in the response of self-grafted (s-g) and salt-tolerant pumpkin-grafted (cucurbita maxima × c. moschata) cucumber seedlings to 80 mm ca(no3 )2 stress. endogenous no and h2 o2 production in s-g seedlings increased in a time-dependent manner, reaching maximum levels after 24 h of ca(no3 )2 stres ... | 2016 | 27935073 |
| the introduction of submitted approaches in iranian traditional medicine for treatment of diabetes. | diabetes mellitus is a complex disorder of carbohydrate metabolism. according to past studies, the word ziabites in iranian traditional medicine (itm) correlate with diabetes. the aim of this study was to identify the definition, therapeutics, and mechanism of diabetes from the resources of itm in order to provide new recommendations for the treatment of diabetes. | 2016 | 27840534 |
| isolation of protein storage vacuoles and their membranes. | protein-storage vacuoles (psvs) are specialized vacuoles that sequester large amounts of storage proteins. during seed development, psvs are formed de novo and/or from preexisting lytic vacuoles. seed psvs can be subdivided into four distinct compartments: membrane, globoid, matrix, and crystalloid. in this chapter, we introduce easy methods for isolation of psvs and their membranes from pumpkin seeds. these methods facilitate the identification and characterization of psv components. | 2017 | 27730610 |
| how do modern pesticide treatments influence the mobility of old incurred ddt contaminations in agricultural soils? | even more than 50 years after the ban of ddt in germany, farmers are still affected by its persistence in contaminated soils. depending on the crop cultivated on such soils, this often leads to low-level residues of ddt and its metabolites dde and ddd ("ddx"), which are perceived as a risk by the food value chain. pesticide formulations used in modern agriculture commonly contain high levels of surfactants, but so far no open-field studies have evaluated the effects of these treatments on the mo ... | 2016 | 27662464 |
| the introduction of submitted approaches in iranian traditional medicine for treatment of diabetes. | diabetes mellitus is a complex disorder of carbohydrate metabolism. according to past studies, the word ziabites in iranian traditional medicine (itm) correlate with diabetes. the aim of this study was to identify the definition, therapeutics, and mechanism of diabetes from the resources of itm in order to provide new recommendations for the treatment of diabetes. | 2016 | 27516697 |
| effects of dietary fiber extracted from pumpkin (cucurbita maxima duch.) on the physico-chemical and sensory characteristics of reduced-fat frankfurters. | in this study, we investigated the effects of reducing fat levels from 30% to 25, 20, and 15% by substituting pork fat with water and pumpkin fiber (2%) on the quality of frankfurters compared with control. decreasing the fat concentration from 30% to 15% significantly increased moisture content, redness of meat batter and frankfurter, cooking loss, and water exudation, and decreased fat content, energy value, ph, and lightness of meat batter and frankfurter, hardness, cohesiveness, gumminess, c ... | 2016 | 27433101 |
| tannic acid alleviates bulk and nanoparticle nd2o3 toxicity in pumpkin: a physiological and molecular response. | the effect of dissolved organic matter (dom) on nanoparticle toxicity to plants is poorly understood. in this study, tannic acid (ta) was selected as a dom surrogate to explore the mechanisms of neodymium oxide nps (nd2o3 nps) phytotoxicity to pumpkin (cucurbita maxima). the results from the tested concentrations showed that 100 mg l(-1) nd2o3 nps were significantly toxic to pumpkin in term of fresh biomass, and the similar results from the bulk particles and the ionic treatments were also evide ... | 2016 | 27308847 |
| systemic induction of no-, redox-, and cgmp signaling in the pumpkin extrafascicular phloem upon local leaf wounding. | cucurbits developed the unique extrafascicular phloem (efp) as a defensive structure against herbivorous animals. mechanical leaf injury was previously shown to induce a systemic wound response in the efp of pumpkin (cucurbita maxima). here, we demonstrate that the phloem antioxidant system and protein modifications by no are strongly regulated during this process. activities of the central antioxidant enzymes dehydroascorbate reductase, glutathione reductase and ascorbate reductase were rapidly ... | 2016 | 26904092 |
| characterization and pathogenicity of alternaria burnsii from seeds of cucurbita maxima (cucurbitaceae) in bangladesh. | in the course of survey of endophytic fungi from bangladesh pumpkin seeds in 2011~2012, two strains (cnu111042 and cnu111043) with similar colony characteristics were isolated and characterized by their morphology and by molecular phylogenetic analysis of the internal transcribed spacer, glyceraldehydes-3-phosphate dehydrogenase (gpd), and alternaria allergen a1 (alt a1) sequences. phylogenetic analysis of all three sequences and their combined dataset revealed that the fungus formed a subclade ... | 2015 | 26839497 |
| sequence variations in the fad2 gene in seeded pumpkins. | seeded pumpkins are important economic crops; the seeds contain various unsaturated fatty acids, such as oleic acid and linoleic acid, which are crucial for human and animal nutrition. the fatty acid desaturase-2 (fad2) gene encodes delta-12 desaturase, which converts oleic acid to linoleic acid. however, little is known about sequence variations in fad2 in seeded pumpkins. twenty-seven fad2 clones from 27 accessions of cucurbita moschata, cucurbita maxima, cucurbita pepo, and cucurbita ficifoli ... | 2015 | 26782391 |
| microbacterium horti sp. nov., a bacterium isolated from cucurbita maxima cultivating soil. | a novel bacterial strain thg-sl1(t) was isolated from a soil sample of cucurbita maxima garden and was characterized by using a polyphasic approach. cells were gram-reaction-positive, non-motile and rod-shaped. the strain was aerobic, catalase positive and weakly positive for oxidase. phylogenetic analysis based on 16s rrna gene sequence analysis but it shared highest similarity with microbacterium ginsengisoli kctc 19189(t) (96.6 %), indicating that strain thg-sl1(t) belongs to the genus microb ... | 2016 | 26757723 |
| a high-density genetic map for anchoring genome sequences and identifying qtls associated with dwarf vine in pumpkin (cucurbita maxima duch.). | pumpkin (cucurbita maxima duch.) is an economically important crop belonging to the cucurbitaceae family. however, very few genomic and genetic resources are available for this species. as part of our ongoing efforts to sequence the pumpkin genome, high-density genetic map is essential for anchoring and orienting the assembled scaffolds. in addition, a saturated genetic map can facilitate quantitative trait locus (qtl) mapping. | 2015 | 26704908 |
| discovering metabolic indices for early detection of squash (cucurbita maxima) storage quality using gc-ms-based metabolite profiling. | squash (cucubita maxima) cultivars with good storage qualities are needed for breeding to improve poor crop supply during winter in japan. we measured changes in squash constituents during different storage periods to identify compounds that were suitable to be used as indices of storage quality. principal components analysis of compounds at 1-5 months after harvest showed that pc1 scores were lower for cultivars with a higher rather than lower sq (storage quality) ranks. partial least-squares r ... | 2016 | 26593601 |
| are trichomes involved in the biomechanical systems of cucurbita leaf petioles? | trichomes are involved in petiole movement and likely function as a part of the plant biomechanical system serving as an additional reservoir of hydrostatic pressure. the large, non-glandular trichomes on cucurbita petioles occur across collenchyma strands. time-lapse imaging was used to study the leaf reorientation of cucurbita maxima 'bambino' plants placed in horizontal position. the experiment comprised four variants of the large non-glandular petiole trichomes: (1) intact, (2) mechanically ... | 2015 | 26306605 |
| biologically synthesised silver nanoparticles from three diverse family of plant extracts and their anticancer activity against epidermoid a431 carcinoma. | biological synthesis of silver nanoparticles is a cost effective natural process where the phytochemicals specifically phenols, flavonoids and terpenoids present in the plant extracts act as capping and reducing agent. due to their nano size regime the silver nanoparticles may directly bind to the dna of the pathogenic bacterial strains leading to higher antimicrobial activity. | 2015 | 26196716 |
| short communication: genetic linkage map of cucurbita maxima with molecular and morphological markers. | cucurbita maxima is one of the most widely cultivated vegetables in china and exhibits distinct morphological characteristics. in this study, genetic linkage analysis with 57 simple-sequence repeats, 21 amplified fragment length polymorphisms, 3 random-amplified polymorphic dna, and one morphological marker revealed 20 genetic linkage groups of c. maxima covering a genetic distance of 991.5 cm with an average of 12.1 cm between adjacent markers. genetic linkage analysis identified the simple-seq ... | 2015 | 26125744 |
| physico-chemical properties and fatty acid composition of pomegranate, cherry and pumpkin seed oils. | nut and seed oils are often considered waste products but in recent years they have been receiving growing interest due to their high concentration of hydrophilic and lipophilic bioactive components, which have important pharmacological properties on human health. the aim of this work was to compare the physico-chemical and biochemical properties of pomegranate (punicagranatum), sweet cherry (prunusavium) and pumpkin (cucurbita maxima) seed oils obtained by solvent extraction. | 2016 | 26033409 |
| a review on omega-3 and omega-6 essential fatty acids: uses, benefits and their availability in pumpkins (cucurbita maxima) seed and desert dates (balanites aegyptiaca) seed kernel oils. | | 2014 | 26027166 |
| high throughput sequencing of small rnas in the two cucurbita germplasm with different sodium accumulation patterns identifies novel micrornas involved in salt stress response. | micrornas (mirnas), a class of small non-coding rnas, recognize their mrna targets based on perfect sequence complementarity. mirnas lead to broader changes in gene expression after plants are exposed to stress. high-throughput sequencing is an effective method to identify and profile small rna populations in non-model plants under salt stresses, significantly improving our knowledge regarding mirna functions in salt tolerance. cucurbits are sensitive to soil salinity, and the cucurbita genus is ... | 2015 | 26010449 |
| evaluation of cucurbita maxima extract against scopolamine-induced amnesia in rats: implication of tumour necrosis factor alpha. | cucurbita maxima (cm) seed oil is commonly used in indian folk medicine to treat various ailments. we have investigated the effect of cm seed oil on memory impairment induced by scopolamine in rats. male adult wistar rats were administered scopolamine 1 mg/kg body weight, i.p. or 1.25 mg/kg body weight, s.c. to induce memory impairment. the nootropic agent piracetam 100 mg/kg body weight, i.p. and cm seed oil 100 and 200 mg/kg body weight, p.o. were administered daily for five consecutive days. ... | 2015 | 25711042 |
| effects of grafting with pumpkin rootstock on carbohydrate metabolism in cucumber seedlings under ca(no3)2 stress. | this study investigated the effects of grafting on the carbohydrate status and the enzymes of carbohydrate metabolism in self-grafted and grafted cucumber seedlings using the salt-tolerant pumpkin rootstock 'qingzhen 1' (cucurbita maxima × cucurbita moschata) under 80 mm ca(no3)2 stress for 6 d. the growth of self-grafted seedlings was significantly inhibited after the treatment of ca(no3)2 stress, whereas the inhibition of growth was alleviated in pumpkin rootstock-grafted seedlings. ca(no3)2 s ... | 2015 | 25579659 |
| selenium accumulating leafy vegetables are a potential source of functional foods. | selenium deficiency in humans has been associated with various diseases, the risks of which can be reduced through dietary supplementation. selenium accumulating plants may provide a beneficial nutrient for avoiding such illnesses. thus, leafy vegetables such as amaranthus hybridus, amaranthus sp., cucurbita maxima, ipomoea batatas, solanum villosum, solanum scabrum, and vigna unguiculata were explored for their capabilities to accumulate selenium when grown on selenium enriched soil and for use ... | 2015 | 26955635 |
| the making of giant pumpkins: how selective breeding changed the phloem of cucurbita maxima from source to sink. | despite the success of breeding programmes focused on increasing fruit size, relatively little is known about the anatomical and physiological changes required to increase reproductive allocation. to address this gap in knowledge, we compared fruit/ovary anatomy, vascular structure and phloem transport of two varieties of giant pumpkins, and their smaller fruited progenitor under controlled environmental conditions. we also modelled carbon transport into the fruit of competitively grown plants u ... | 2015 | 25546629 |
| wheat bread with pumpkin (cucurbita maxima l.) pulp
as a functional food product. | in this study, a new application of pumpkin pulp in bread production is shown. the aim of this work is to determine the influence of the addition of fresh pumpkin pulp directly into wheat flour on physical, sensorial and biological properties of bread. the bioaccessibility of active compounds was also studied. an increase in the addition of pumpkin pulp from 5 to 20% (converted to dry matter) caused a decrease of bread volume and increase of crumb hardness and cohesiveness. the sensory character ... | 2014 | 27904316 |
| 11s storage globulin from pumpkin seeds: regularities of proteolysis by papain. | limited proteolysis of the α- and β-chains and deep cleavage of the αβ-subunits by the cooperative (one-by-one) mechanism was observed in the course of papain hydrolysis of cucurbitin, an 11s storage globulin from seeds of the pumpkin cucurbita maxima. an independent analysis of the kinetics of the limited and cooperative proteolyses revealed that the reaction occurs in two successive steps. in the first step, limited proteolysis consisting of detachments of short terminal peptides from the α- a ... | 2014 | 25365492 |
| systemic delivery of sirna in pumpkin by a plant phloem small rna-binding protein 1-ribonucleoprotein complex. | in plants, the vascular system, specifically the phloem, functions in delivery of small rna (srna) to exert epigenetic control over developmental and defense-related processes. although the importance of systemic srna delivery has been established, information is currently lacking concerning the nature of the protein machinery involved in this process. here, we show that a phloem small-rna binding protein 1 (psrp1) serves as the basis for formation of an srna ribonucleoprotein complex (srnpc) th ... | 2014 | 25227635 |
| description of ektaphelenchoides fuchsi n. sp. (nematoda: ektaphelenchinae) from western iran. | ektaphelenchoides fuchsi n. sp., recovered from a soil sample around the rhizosphere of cucurbita maxima in western iran, is described and illustrated based on morphological and molecular characters. the new species is characterized by its body length of 529-712 µm, continuous lip region, lateral fields with three incisures, total stylet length of 14-18 µm with rounded basal knobs, excretory pore 70-84 µm and hemizonid 87-96 µm from the anterior end, post-uterine sac short, 6-9 µm long in female ... | 2014 | 25112260 |
| pumpkin seed oil extracted from cucurbita maxima improves urinary disorder in human overactive bladder. | the pumpkin seed oil obtained from cucurbita pepo has been shown to be useful for the treatment of nocturia in patients with urinal disorders in several western countries. in this study, we evaluated the effect of the pumpkin seed oil from cucurbita maxima on urinary dysfunction in human overactive bladder (oab). forty-five subjects were enrolled in this study. an extract of pumpkin seed oil from c. maxima (10 g of oil/day) was orally administrated for 12 weeks. after 6 and 12 weeks, urinary fun ... | 2014 | 24872936 |
| three new triterpene esters from pumpkin (cucurbita maxima) seeds. | three new multiflorane-type triterpene esters, i.e. 7α-hydroxymultiflor-8-ene-3α,29-diol 3-acetate-29-benzoate (1), 7α-methoxymultiflor-8-ene-3α,29-diol 3,29-dibenzoate (2), and 7β-methoxymultiflor-8-ene-3α,29-diol 3,29-dibenzoate (3), were isolated from seeds of cucurbita maxima, along with the known compound, multiflora-7,9(11)-diene-3α,29-diol 3,29-dibenzoate (4). compound 1 exhibited melanogenesis inhibitory activities comparable with those of arbutin. in cytotoxicity assays, compounds 1 and ... | 2014 | 24743937 |
| direct and individual analysis of stress-related phytohormone dispersion in the vascular system of cucurbita maxima after flagellin 22 treatment. | • the stress-related phytohormones, salicylic acid (sa) and abscisic acid (aba), and the three jasmonates, jasmonic acid (ja), cis-12-oxo-phytodienoic acid (cis-opda), and (+)-7-iso-jasmonoyl-l-isoleucine (ja-ile), were investigated in phloem and xylem exudates of cucurbita maxima. • phloem and xylem exudates were separately collected and analysed via liquid chromatography-mass spectrometry. • we show direct evidence for all three jasmonates, aba, and sa in both phloem and xylem exudates of c. m ... | 2014 | 24387138 |
| antioxidant enzymatic activities and gene expression associated with heat tolerance in the stems and roots of two cucurbit species ("cucurbita maxima" and "cucurbita moschata") and their interspecific inbred line "maxchata". | the elucidation of heat tolerance mechanisms is required to combat the challenges of global warming. this study aimed to determine the antioxidant enzyme responses to heat stress, at the enzymatic activity and gene expression levels, and to investigate the antioxidative alterations associated with heat tolerance in the stems and roots of squashes using three genotypes differing in heat tolerance. plants of heat-tolerant "c. moschata", thermolabile "c. maxima" and moderately heat-tolerant intersp ... | 2013 | 24336062 |
| characteristics of organic acids in the fruit of different pumpkin species. | the aim of the research was to determine the composition of organic acids in fruit of different cultivars of three pumpkin species. the amount of acids immediately after fruit harvest and after 3 months of storage was compared. the content of organic acids in the examined pumpkin cultivars was assayed using the method of high performance liquid chromatography (hplc). three organic acids (citric acid, malic acid, and fumaric acid) were identified in the cultivars, whose content considerably varie ... | 2014 | 24262577 |
| microwave-assisted aqueous enzymatic extraction of oil from pumpkin seeds and evaluation of its physicochemical properties, fatty acid compositions and antioxidant activities. | microwave-assisted aqueous enzymatic extraction (maaee) of pumpkin seed oil was performed in this study. an enzyme cocktail comprised of cellulase, pectinase and proteinase (w/w/w) was found to be the most effective in releasing oils. the highest oil recovery of 64.17% was achieved under optimal conditions of enzyme concentration (1.4%, w/w), temperature (44°c), time (66 min) and irradiation power (419w). moreover, there were no significant variations in physicochemical properties of maaee-extra ... | 2014 | 24206680 |
| metabolites of 2,4,4'-tribrominated diphenyl ether (bde-28) in pumpkin after in vivo and in vitro exposure. | there is currently limited knowledge on pbde metabolism in plants although they could play an important role in the environmental transformation of these persistent organic pollutants. in this study, pumpkin (cucurbita maxima × c. moschata) was chosen as the model to understand the fate of bde-28 in plants. meo-tri-bdes, oh-tri-bdes, and oh-tri-bdes were found as metabolites in plant samples of both in vivo hydroponic and in vitro tissue culture exposure. three meo-tri-bdes were further identifi ... | 2013 | 24191731 |
| pumpkin (cucurbita maxima) seed proteins: sequential extraction processing and fraction characterization. | seed proteins extracted from tunisian pumpkin seeds ( cucurbita maxima ) were investigated for their solubility properties and sequentially extracted according to the osborne procedure. the solubility of pumpkin proteins from seed flour was greatly influenced by ph changes and ionic strength, with higher values in the alkaline ph regions. it also depends on the seed defatting solvent. protein solubility was decreased by using chloroform/methanol (cm) for lipid extraction instead of pentane (p). ... | 2013 | 23869935 |
| three new multiflorane-type triterpenes from pumpkin (cucurbita maxima) seeds. | three new multiflorane-type triterpenes; 7a-methoxymultiflor-8-ene-3a,29-diol 3-acetate-29-benzoate (1), 7-oxomultiflor-8-ene-3a,29-diol 3-acetate-29-benzoate (2), and multiflora-7,9(11)-diene-3a,29-diol 3-p-hydroxybenzoate-29-benzoate (3), were isolated from seeds of cucurbita maxima, along with three known compounds. compound 3 and multiflora-7,9(11)-diene-3a-29-diol 3-benzoate (5) exhibited potent inhibitory effects on melanogenesis, with low cytotoxicities, and 2 exhibited single-digit micro ... | 2013 | 23673529 |
| characteristics of antioxidant activity and composition of pumpkin seed oils in 12 cultivars. | the objective of this study was to determine the antioxidant properties, and provide characteristics, of the oil obtained from the seeds of 12 pumpkin varieties belonging to the species cucurbita maxima duch. and cucurbita pepo l. another objective was to establish which of the two extracting agents, ethanol or methanol, is more effective. the seeds of the pumpkin varieties examined differ in chemical composition and antioxidant activity. the seeds of the cultivars belonging to the species c. ma ... | 2013 | 23561092 |
| in vivo metabolism of 2,2',4,4'-tetrabromodiphenyl ether (bde-47) in young whole pumpkin plant. | polybrominated diphenyl ethers (pbdes) are widely distributed persistent organic pollutants. in vitro and in vivo research using various animal models have shown that pbdes might be transformed to hydroxylated pbdes, but there are few studies on in vivo metabolism of pbdes by intact whole plants. in this research, pumpkin plants (cucurbita maxima × c. moschata) were hydroponically exposed to 2,2',4,4'-tetrabromodiphenyl ether (bde-47). a debromination product (bde-28) and four hydroxylated metab ... | 2013 | 23510101 |
| dissoconiaceae associated with sooty blotch and flyspeck on fruits in china and the united states. | zasmidium angulare, a novel species of mycosphaerellaceae, and several novel taxa that reside in dissoconiaceae, were identified from a collection of apples and cucurbita maxima (cv. blue hubbard) from china and the usa that exhibited sooty blotch and flyspeck (sbfs) signs on their host substrata. morphology on fruit surfaces and in culture, and phylogenetic analyses of the nuclear ribosomal dnas 28s and internal transcribed spacer regions, as well as partial translation elongation factor 1-alph ... | 2012 | 23105157 |
| deciphering systemic wound responses of the pumpkin extrafascicular phloem by metabolomics and stable isotope-coded protein labeling. | in cucurbits, phloem latex exudes from cut sieve tubes of the extrafascicular phloem (efp), serving in defense against herbivores. we analyzed inducible defense mechanisms in the efp of pumpkin (cucurbita maxima) after leaf damage. as an early systemic response, wounding elicited transient accumulation of jasmonates and a decrease in exudation probably due to partial sieve tube occlusion by callose. the energy status of the efp was enhanced as indicated by increased levels of atp, phosphate, and ... | 2012 | 23085839 |
| interaction of xylem and phloem during exudation and wound occlusion in cucurbita maxima. | collection of cucurbit exudates from cut petioles has been a powerful tool for gaining knowledge on phloem sap composition without full notion of the complex exudation mechanism. only few publications explicitly mentioned that exudates were collected from the basal side of the cut, which exudes more copiously than the apical side. this is surprising since only exudation from the apical side is supposedly driven by phloem pressure gradients. composition of carbohydrates and ph values at both woun ... | 2013 | 22765252 |
| comparative analysis of cells and proteins of pumpkin plants for the control of fruit size. | common pumpkin plants (cucurbita maxima) produce fruits of 1-2 kg size on the average, while special varieties of the same species called atlantic giant are known to produce a huge fruit up to several hundred kilograms. as an approach to determine the factors controlling the fruit size in c. maxima, we cultivated both ag and control common plants, and found that both the cell number and cell sizes were increased in a large fruit while dna content of the cell did not change significantly. we also ... | 2012 | 22677066 |
| carotenoid accumulation and carotenogenic gene expression during fruit development in novel interspecific inbred squash lines and their parents. | carotenoid levels and composition during squash fruit development were compared in cucurbita moschata , cucurbita maxima , and two lines of their interspecific inbred lines, namely, maxchata1 and maxchata2. eight genes associated with carotenoid biosynthesis were analyzed by quantitative rt-pcr. the two squash species and their interspecific inbred lines exhibited different qualitative and quantitative carotenoid profiles and regulatory mechanisms. c. moschata had the lowest total carotenoid con ... | 2012 | 22574777 |
| transgenic tobacco plants as production platform for biologically active human interleukin 2 and its fusion with proteinase inhibitors. | transgenic plants offer a low-cost approach for the production of pharmaceutically important and commercially valuable recombinant proteins. our studies were focused on the plant-based production of human interleukin 2 (hil-2) and its fusion with proteinase inhibitors, either spi2 from galleria mellonella or cmti from cucurbita maxima. finally, five plant expression cassettes were obtained. three of them contained the single cdna encoding cmti i, spi2 and hil-2, respectively, while two of them c ... | 2012 | 22564275 |
| in vivo quantification of cell coupling in plants with different phloem-loading strategies. | uptake of photoassimilates into the leaf phloem is the key step in carbon partitioning and phloem transport. symplasmic and apoplasmic loading strategies have been defined in different plant taxa based on the abundance of plasmodesmata between mesophyll and phloem. for apoplasmic loading to occur, an absence of plasmodesmata is a sufficient but not a necessary criterion, as passage of molecules through plasmodesmata might well be blocked or restricted. here, we present a noninvasive, whole-plant ... | 2012 | 22422939 |
| stamen-derived bioactive gibberellin is essential for male flower development of cucurbita maxima l. | gibberellin (ga) signalling during pumpkin male flower development is highly regulated, including biosynthetic, perception, and transduction pathways. ga 20-oxidases, 3-oxidases, and 2-oxidases catalyse the final part of ga synthesis. additionally, 7-oxidase initiates this part of the pathway in some cucurbits including cucurbita maxima l. (pumpkin). expression patterns for these ga-oxidase-encoding genes were examined by competitive reverse transcription-pcr (rt-pcr) and endogenous ga levels we ... | 2012 | 22268154 |
| content of bioactive compounds and antioxidant capacity of pumpkin puree enriched with japanese quince, cornelian cherry, strawberry and apples. | when evaluated in terms of taste, smell or active ingredients, pumpkin in itself is not very attractive as a raw material. hence, it is recommended to blend pumpkin with other fruits, which are aromatic, have a defined taste, and contain a large quantity of active ingredients and organic acids to improve its palatibility. | 2013 | 22232528 |
| quantification of plant cell coupling with three-dimensional photoactivation microscopy. | plant cells are directly connected by plasmodesmata that form channels through the cell wall and enable the intercellular movement of cytosolic solutes, membrane lipids and signalling molecules. transport through plasmodesmata is regulated not only by a fixed size-exclusion limit, but also by physiological and pathological adaptation. to understand plant cell communication, carbon allocation and pathogen attack, the capacities for a specific molecule to pass a specific cell-wall interface is an ... | 2012 | 22171617 |
| changes in carotenoids during processing and storage of pumpkin puree. | changes in the contents of carotenoids and their true retentions (% tr) during the production of puree of cucurbita moschata 'menina brasileira' and of cucurbita maxima 'exposição' pumpkins and the stability of such compounds during 180days of storage were monitored by liquid chromatography coupled with a photodiode array detector. cooking caused higher losses than commercial sterilisation. high losses of xanthophylls such as lutein and violaxanthin were noted during processing and storage of pu ... | 2011 | 25214348 |
| comparison of waste pumpkin material and its potential use in extruded snack foods. | material was produced from crown pumpkin (cucurbita maxima) processed from fractions of the fruit which are regarded as waste stream products (peel, flesh and seed). the flour from the three different fractions (peel, flesh and seed) of crown pumpkin flour was incorporated into an extruded snack product formulation at levels 10%, 30% and 50% (w/w with corn grit) and processed in a twin-screw extruder to make 10 expanded snack products. proximate analysis was carried out to determine the nutritio ... | 2011 | 21813596 |
| an in vitro evaluation of anthelmintic activity of zingiber zerumbet rhizomes and cucurbita maxima seeds on pheretima posthuma model: a comparative study. | | 2011 | 21687369 |
| effects of cooking on the cell walls (dietary fiber) of 'scarlet warren' winter squash ( cucurbita maxima ) studied by polysaccharide linkage analysis and solid-state (13)c nmr. | cell wall polysaccharides of 'scarlet warren' winter squash ( cucurbita maxima ) were investigated before and after thermal processing. linkage analysis of polysaccharides was done by gas chromatography coupled to mass spectrometry (gc-ms). the linkage analysis showed the cell wall polysaccharide compositions of raw and cooked squash were similar. the total pectic polysaccharides (galacturonan, rhamnogalacturonan, arabinan, and arabinogalactan) contents of the cell walls of both raw and cooked s ... | 2011 | 21604813 |
| cmrbp50 protein phosphorylation is essential for assembly of a stable phloem-mobile high-affinity ribonucleoprotein complex. | rna-binding proteins (rbps) form ribonucleoprotein (rnp) complexes that play crucial roles in rna processing for gene regulation. the angiosperm sieve tube system contains a unique population of transcripts, some of which function as long-distance signaling agents involved in regulating organ development. these phloem-mobile mrnas are translocated as rnp complexes. one such complex is based on a phloem rbp named cucurbita maxima rna-binding protein 50 (cmrbp50), a member of the polypyrimidine tr ... | 2011 | 21572046 |
| isothermal titration calorimetric and computational studies on the binding of chitooligosaccharides to pumpkin (cucurbita maxima) phloem exudate lectin. | the interaction of chitooligosaccharides [(glcnac)(2-6)] with pumpkin phloem exudate lectin (ppl) was investigated by isothermal titration calorimetry and computational methods. the dimeric ppl binds to (glcnac)(3-5) with binding constants of 1.26-1.53 × 10(5) m(-1) at 25 °c, whereas chitobiose exhibits approximately 66-fold lower affinity. interestingly, chitohexaose shows nearly 40-fold higher affinity than chitopentaose with a binding constant of 6.16 × 10(6) m(-1). the binding stoichiometry ... | 2011 | 21405077 |
| differential spatial expression of a- and b-type cdks, and distribution of auxins and cytokinins in the open transverse root apical meristem of cucurbita maxima. | aside from those on arabidopsis, very few studies have focused on spatial expression of cyclin-dependent kinases (cdks) in root apical meristems (rams), and, indeed, none has been undertaken for open meristems. the extent of interfacing between cell cycle genes and plant growth regulators is also an increasingly important issue in plant cell cycle studies. here spatial expression/localization of an a-type and b-type cdk, auxin and cytokinins are reported in relation to the hitherto unexplored an ... | 2011 | 20601387 |
| response of epilachna paenulata to two flavonoids, pinocembrin and quercetin, in a comparative study. | we examined the effects of the flavonoids pinocembrin and quercetin on the feeding behavior, survival, and development of the cucurbitaceae pest epilachna paenulata (coleoptera: coccinellidae). in no-choice experiments, 48 hr-consumption of cucurbita maxima duch. leaves treated with pinocembrin at 1, 5, and 50 microg/cm(2) was less than one third of that for leaves treated with 0.1 microg/cm(2) of pinocembrin or untreated leaves. larvae stopped feeding after 9 days of high doses of pinocembrin ( ... | 2010 | 20589417 |
| remote-controlled stop of phloem mass flow by biphasic occlusion in cucurbita maxima. | the relationships between damage-induced electropotential waves (epws), sieve tube occlusion, and stop of mass flow were investigated in intact cucurbita maxima plants. after burning leaf tips, epws propagating along the phloem of the main vein were recorded by extra- and intracellular microelectrodes. the respective epw profiles (a steep hyperpolarization/depolarization peak followed by a prolonged hyperpolarization/depolarization) probably reflect merged action and variation potentials. a few ... | 2010 | 20584788 |
| sieve tube geometry in relation to phloem flow. | sieve elements are one of the least understood cell types in plants. translocation velocities and volume flow to supply sinks with photoassimilates greatly depend on the geometry of the microfluidic sieve tube system and especially on the anatomy of sieve plates and sieve plate pores. several models for phloem translocation have been developed, but appropriate data on the geometry of pores, plates, sieve elements, and flow parameters are lacking. we developed a method to clear cells from cytopla ... | 2010 | 20354199 |
| random estimate the values of seed oil of cucurbita maxima by refractive index method. | the crude oil having lower iodine and free fatty acids values has aamdosha properties. these properties are present due to toxic and anti-toxic compounds. these compounds can be harmful for the special diseases and may be unsaturated, saturated, open chain etc. the adulteration can take part as catalytic action for the toxic effect for the special diseases. toxic properties of oils are removed by different ingrediants and methods. c. maxima seed tail (mst) is used with food and medicine. the pre ... | 2010 | 22131677 |
| rapid affinity-purification and physicochemical characterization of pumpkin (cucurbita maxima) phloem exudate lectin. | the chito-oligosaccharide-specific lectin from pumpkin (cucurbita maxima) phloem exudate has been purified to homogeneity by affinity chromatography on chitin. after sds/page in the presence of 2-mercaptoethanol, the pumpkin phloem lectin yielded a single band corresponding to a molecular mass of 23.7 kda, whereas esi-ms (electrospray ionization ms) gave the molecular masses of the subunit as 24645 da. analysis of the cd spectrum of the protein indicated that the secondary structure of the lecti ... | 2010 | 19735229 |
| tryptophan exposure and accessibility in the chitooligosaccharide-specific phloem exudate lectin from pumpkin (cucurbita maxima). a fluorescence study. | the exposure and accessibility of the tryptophan residues in the chitooligosaccharide-specific pumpkin (cucurbita maxima) phloem exudate lectin (ppl) have been investigated by fluorescence spectroscopy. the emission lambda(max) of native ppl, seen at 338nm was red-shifted to 348nm upon denaturation by 6m gdn.hcl in the presence of 10mm beta-mercaptoethanol, indicating near complete exposure of the tryptophan residues to the aqueous medium, whereas a blue-shift to 335nm was observed in the presen ... | 2009 | 19700341 |
| antioxidative activity of water soluble polysaccharide in pumpkin fruits (cucurbita maxima duchesne). | we evaluated the antioxidative activity of a water soluble polysaccharide fraction (wsp) from pumpkin fruits (cucurbita maxima duchesne). in the wsp, dpph radical scavenging and superoxide dismutase-like activity increased depending on the total sugar content. furthermore, the wsp can serve as an inhibitor of ascorbic acid oxidation. the efficacy was also affected by the total sugar content. | 2009 | 19502750 |
| extraction of ascorbate oxidase from cucurbita maxima by continuous process in perforated rotating disc contactor using aqueous two-phase systems. | the ascorbate oxidase is the enzyme used to determine the content of ascorbic acid in the pharmaceutical and food industries and clinics analyses. the techniques currently used for the purification of this enzyme raise its production cost. thus, the development of alternative processes and with the potential to reduce costs is interesting. the application of aqueous two-phase system is proposed as an alternative to purification because it enables good separation of biomolecules. the objective of ... | 2010 | 19412579 |
| exact and effective pair-wise potential for protein-ligand interactions obtained from a semiempirical energy partition. | in this work, the partition method introduced by carvalho and melo was used to study the complex between cucurbita maxima trypsin inhibitor (cmti-i) and glycerol at the am1 level. an effective potential, combining non-bonding and polarization plus charge transfer (plct) terms, was introduced to evaluate the magnitude of the interaction between each amino acid and the ligand. in this case study, the nonbonding-plct non-compensation characterizes the stabilization energy of the association process ... | 2008 | 19325823 |
| the phloem-delivered rna pool contains small noncoding rnas and interferes with translation. | in plants, the vascular tissue contains the enucleated sieve tubes facilitating long-distance transport of nutrients, hormones, and proteins. in addition, several mrnas and small interfering rnas/micrornas were shown to be delivered via sieve tubes whose content is embodied by the phloem sap (ps). a number of these phloem transcripts are transported from source to sink tissues and function at targeted tissues. to gain additional insights into phloem-delivered rnas and their potential role in sig ... | 2009 | 19261735 |
| a polypyrimidine tract binding protein, pumpkin rbp50, forms the basis of a phloem-mobile ribonucleoprotein complex. | rna binding proteins (rbps) are integral components of ribonucleoprotein (rnp) complexes and play a central role in rna processing. in plants, some rbps function in a non-cell-autonomous manner. the angiosperm phloem translocation stream contains a unique population of rbps, but little is known regarding the nature of the proteins and mrna species that constitute phloem-mobile rnp complexes. here, we identified and characterized a 50-kd pumpkin (cucurbita maxima cv big max) phloem rna binding pr ... | 2009 | 19122103 |
| evaluation of alternatives to methyl bromide in melon crops in guatemala. | the monoculture of melon in guatemala has caused the massive appearance of plants with an analogous syndrome for the well-known disease commonly called melon collapse, or vine decline, causing significant losses in crops. methyl bromide is commonly used to sterilize soil prior to planting in guatemala, but it must be phased out by 2015. the objective of this study was to evaluate the technique of grafting melon onto hybrids of cucurbita (cucurbita maxima x cucurbita moschata), as an alternative ... | 2009 | 18998189 |
| hplc-dad-ms(n) characterisation of carotenoids from apricots and pumpkins for the evaluation of fruit product authenticity. | carotenoids including carotenoid esters from six apricot (prunus armeniaca l.) cultivars and from eight cultivars from three pumpkin species (cucurbita maxima duch., cucurbita pepo l., and cucurbita moschata duch.) were extracted without saponification, separated on a c-30 reversed-phase column and characterised by high-performance liquid chromatography/atmospheric pressure chemical ionisation-mass spectrometry (lc-ms). the predominant free carotenoids were quantified by hplc with diode array de ... | 2008 | 26049248 |
| qualitative and quantitative differences in carotenoid composition among cucurbita moschata, cucurbita maxima, and cucurbita pepo. | squashes and pumpkins are important dietary sources of carotenoids worldwide. the carotenoid composition has been determined, but reported data have been highly variable, both qualitatively and quantitatively. in the present work, the carotenoid composition of squashes and pumpkins currently marketed in campinas, brazil, were determined by hplc-dad, complemented by hplc-ms for identification. cucurbita moschata 'menina brasileira' and c. moschata 'goianinha' had similar profiles, with beta-carot ... | 2007 | 17444652 |
| differences in p,p'-dde bioaccumulation from compost and soil by the plants cucurbita pepo and cucurbita maxima and the earthworms eisenia fetida and lumbricus terrestris. | two plant species, cucurbita pepo and cucurbita maxima, and two earthworm species, eisenia fetida and lumbricus terrestris, were exposed to soil and compost with equivalent p,p'-dde contamination. pollutant bioconcentration was equal in plant roots in both media, but translocation was higher in c. pepo. bioaccumulation by e. fetida was approximately 6- and 3-fold higher than that by l. terrestris in the soil and compost, respectively. for all species, p,p'-dde uptake was significantly greater fr ... | 2007 | 17241722 |
| kinetic and thermodynamic investigation on ascorbate oxidase activity and stability of a cucurbita maxima extract. | the kinetic and thermodynamic properties of ascorbate oxidase (ao) activity and stability of a cucurbita maxima extract were investigated. activity tests performed at 25 degrees c using initial ascorbic acid concentration in the range 50-750 m allowed estimating the michaelis constant for this substrate (km = 126 microm) and the maximum initial rate of ascorbic acid oxidation (a0,max = 1.57 mm min-1). the main thermodynamic parameters of the enzyme reaction (deltah* = 10.3 kj mol-1; deltag* = 87 ... | 2006 | 17137312 |
| the squash aspartic proteinase inhibitor sqapi is widely present in the cucurbitales, comprises a small multigene family, and is a member of the phytocystatin family. | the squash (cucurbita maxima) phloem exudate-expressed aspartic proteinase inhibitor (sqapi) is a novel aspartic acid proteinase inhibitor, constituting a fifth family of aspartic proteinase inhibitors. however, a comparison of the sqapi sequence to the phytocystatin (a cysteine proteinase inhibitor) family sequences showed approximately 30% identity. modeling sqapi onto the structure of oryzacystatin gave an excellent fit; regions identified as proteinase binding loops in cystatin coincided wit ... | 2006 | 17103059 |
| gibberellin biosynthesis in developing pumpkin seedlings. | a gibberellin (ga) biosynthetic pathway was discovered operating in root tips of 7-d-old pumpkin (cucurbita maxima) seedlings. stepwise analysis of ga metabolism in cell-free systems revealed the conversion of ga(12)-aldehyde to bioactive ga(4) and inactive ga(34). highest levels of endogenous ga(4) and ga(34) were found in hypocotyls and root tips of 3-d-old seedlings. cdna molecules encoding two ga oxidases, cmga20ox3 and cmga3ox3, were isolated from root tips of 7-d-old lab150978-treated seed ... | 2005 | 16126862 |