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are trichomes involved in the biomechanical systems of cucurbita leaf petioles?trichomes are involved in petiole movement and likely function as a part of the plant biomechanical system serving as an additional reservoir of hydrostatic pressure. the large, non-glandular trichomes on cucurbita petioles occur across collenchyma strands. time-lapse imaging was used to study the leaf reorientation of cucurbita maxima 'bambino' plants placed in horizontal position. the experiment comprised four variants of the large non-glandular petiole trichomes: (1) intact, (2) mechanically ...201526306605
an acorn squash (cucurbita pepo ssp. ovifera) fruit and seed transcriptome as a resource for the study of fruit traits in cucurbita.acorn squash (cucurbita pepo) is an iconic fall vegetable in the united states, known for its unique fruit shape and also prized for its culinary properties. little is known about the metabolism that underlies the development of fruit quality attributes such as color, sweetness, texture and nutritional qualities in acorn squash, or any other winter squash grown worldwide. to provide insight into winter squash fruit and seed development and add to the genomic resources in the cucurbita genus, rna ...201526504561
discovering metabolic indices for early detection of squash (cucurbita maxima) storage quality using gc-ms-based metabolite profiling.squash (cucubita maxima) cultivars with good storage qualities are needed for breeding to improve poor crop supply during winter in japan. we measured changes in squash constituents during different storage periods to identify compounds that were suitable to be used as indices of storage quality. principal components analysis of compounds at 1-5 months after harvest showed that pc1 scores were lower for cultivars with a higher rather than lower sq (storage quality) ranks. partial least-squares r ...201626593601
a high-density genetic map for anchoring genome sequences and identifying qtls associated with dwarf vine in pumpkin (cucurbita maxima duch.).pumpkin (cucurbita maxima duch.) is an economically important crop belonging to the cucurbitaceae family. however, very few genomic and genetic resources are available for this species. as part of our ongoing efforts to sequence the pumpkin genome, high-density genetic map is essential for anchoring and orienting the assembled scaffolds. in addition, a saturated genetic map can facilitate quantitative trait locus (qtl) mapping.201526704908
microbacterium horti sp. nov., a bacterium isolated from cucurbita maxima cultivating soil.a novel bacterial strain thg-sl1(t) was isolated from a soil sample of cucurbita maxima garden and was characterized by using a polyphasic approach. cells were gram-reaction-positive, non-motile and rod-shaped. the strain was aerobic, catalase positive and weakly positive for oxidase. phylogenetic analysis based on 16s rrna gene sequence analysis but it shared highest similarity with microbacterium ginsengisoli kctc 19189(t) (96.6 %), indicating that strain thg-sl1(t) belongs to the genus microb ...201626757723
sequence variations in the fad2 gene in seeded pumpkins.seeded pumpkins are important economic crops; the seeds contain various unsaturated fatty acids, such as oleic acid and linoleic acid, which are crucial for human and animal nutrition. the fatty acid desaturase-2 (fad2) gene encodes delta-12 desaturase, which converts oleic acid to linoleic acid. however, little is known about sequence variations in fad2 in seeded pumpkins. twenty-seven fad2 clones from 27 accessions of cucurbita moschata, cucurbita maxima, cucurbita pepo, and cucurbita ficifoli ...201526782391
characterization and pathogenicity of alternaria burnsii from seeds of cucurbita maxima (cucurbitaceae) in bangladesh.in the course of survey of endophytic fungi from bangladesh pumpkin seeds in 2011~2012, two strains (cnu111042 and cnu111043) with similar colony characteristics were isolated and characterized by their morphology and by molecular phylogenetic analysis of the internal transcribed spacer, glyceraldehydes-3-phosphate dehydrogenase (gpd), and alternaria allergen a1 (alt a1) sequences. phylogenetic analysis of all three sequences and their combined dataset revealed that the fungus formed a subclade ...201526839497
herbivore-triggered electrophysiological reactions: candidates for systemic signals in higher plants and the challenge of their identification.in stressed plants, electrophysiological reactions (elrs) are presumed to contribute to long-distance intercellular communication between distant plant parts. because of the focus on abiotic stress-induced elrs in recent decades, biotic stress-triggered elrs have been widely ignored. it is likely that the challenge to identify the particular elr types (action potential [ap], variation potential, and system potential [sp]) was responsible for this course of action. thus, this survey focused on in ...201626872949
systemic induction of no-, redox-, and cgmp signaling in the pumpkin extrafascicular phloem upon local leaf wounding.cucurbits developed the unique extrafascicular phloem (efp) as a defensive structure against herbivorous animals. mechanical leaf injury was previously shown to induce a systemic wound response in the efp of pumpkin (cucurbita maxima). here, we demonstrate that the phloem antioxidant system and protein modifications by no are strongly regulated during this process. activities of the central antioxidant enzymes dehydroascorbate reductase, glutathione reductase and ascorbate reductase were rapidly ...201626904092
electrophysiology of pumpkin seeds: memristors in vivo.leon chua, the discoverer of a memristor, theoretically predicted that voltage gated ion channels can be memristors. we recently found memristors in different plants such as the venus flytrap, mimosa pudica, aloe vera, apple fruits, and in potato tubers. there are no publications in literature about the existence of memristors in seeds. the goal of this work was to discover if pumpkin seeds might have memristors. we selected cucurbita pepo l., cv. cinderella, cucurbita maxima l. cv warty goblin, ...201626926652
selenium accumulating leafy vegetables are a potential source of functional foods.selenium deficiency in humans has been associated with various diseases, the risks of which can be reduced through dietary supplementation. selenium accumulating plants may provide a beneficial nutrient for avoiding such illnesses. thus, leafy vegetables such as amaranthus hybridus, amaranthus sp., cucurbita maxima, ipomoea batatas, solanum villosum, solanum scabrum, and vigna unguiculata were explored for their capabilities to accumulate selenium when grown on selenium enriched soil and for use ...201526955635
proteomics and metabolomics analyses reveal the cucurbit sieve tube system as a complex metabolic space.the plant vascular system, and specifically the phloem, plays a pivotal role in allocation of fixed carbon to developing sink organs. although the processes involved in loading and unloading of sugars and amino acids are well characterized, little information is available regarding the nature of other metabolites in the sieve tube system (sts) at specific sites along the pathway. here, we elucidate spatial features of metabolite composition mapped with phloem enzymes along the cucurbit sts. phlo ...201627155400
improved purification and spectroscopic properties of squash glutamate decarboxylase.squash glutamate decarboxylase was purified by deae-cellulose batchwise followed by blue-sepharose, cellulofine gcl-2000, and toyopearl hw-55f column chromatography. the purified glutamate decarboxylase had a high specific activity (95.0 u/mg). the absorption spectrum of glutamate decarboxylase had an absorption maximum at 420 nm in the range 300-500 nm. a ph change from 5.3 to 7.8 was accompanied by a decrease in absorbancy at 420 nm. one mole of glutamate decarboxylase contained 3.8 and 1.3 mo ...199627281150
effects of 3-methylgibberellin analogs on gibberellin 3β-hydroxylases and plant growth.six 3-methylgibberellin analogs were synthesized, and their effects on the ga 3β-hydroxylases from immature seeds of phaseolus vulgaris and cucurbita maxima, and/or on the growth of dwarf rice (oryza sativa l. cv. tan-ginbozu) and cucumber (cucumis sativus l. cv. spacemaster) were investigated. 3-methyl-ga5 and 2, 3-didehydro-3-methyl-ga9· inhibited the conversion of [2, 3-(3)h2]ga9 to [2-(3)h]ga4 by ga 3β-hydroxylases from both p. vulgaris and c. maxima at 3 μm and higher. their c/d-ring-rearra ...199227286375
tannic acid alleviates bulk and nanoparticle nd2o3 toxicity in pumpkin: a physiological and molecular response.the effect of dissolved organic matter (dom) on nanoparticle toxicity to plants is poorly understood. in this study, tannic acid (ta) was selected as a dom surrogate to explore the mechanisms of neodymium oxide nps (nd2o3 nps) phytotoxicity to pumpkin (cucurbita maxima). the results from the tested concentrations showed that 100 mg l(-1) nd2o3 nps were significantly toxic to pumpkin in term of fresh biomass, and the similar results from the bulk particles and the ionic treatments were also evide ...201627308847
effects of dietary fiber extracted from pumpkin (cucurbita maxima duch.) on the physico-chemical and sensory characteristics of reduced-fat frankfurters.in this study, we investigated the effects of reducing fat levels from 30% to 25, 20, and 15% by substituting pork fat with water and pumpkin fiber (2%) on the quality of frankfurters compared with control. decreasing the fat concentration from 30% to 15% significantly increased moisture content, redness of meat batter and frankfurter, cooking loss, and water exudation, and decreased fat content, energy value, ph, and lightness of meat batter and frankfurter, hardness, cohesiveness, gumminess, c ...201627433101
comparative proteomics of cucurbit phloem indicates both unique and shared sets of proteins.cucurbits are well-studied models for phloem biology but unusually possess both fascicular phloem (fp) within vascular bundles and additional extrafascicular phloem (efp). although the functional differences between the two systems are not yet clear, sugar analysis and limited protein profiling have established that fp and efp have divergent compositions. here we report a detailed comparative proteomics study of fp and efp in two cucurbits, pumpkin and cucumber. we re-examined the sites of exuda ...201627472661
the introduction of submitted approaches in iranian traditional medicine for treatment of diabetes.diabetes mellitus is a complex disorder of carbohydrate metabolism. according to past studies, the word ziabites in iranian traditional medicine (itm) correlate with diabetes. the aim of this study was to identify the definition, therapeutics, and mechanism of diabetes from the resources of itm in order to provide new recommendations for the treatment of diabetes.201627516697
sterols of cucurbitaceae: the configurations at c-24 of 24-alkyl-δ(5)-,δ(7)- and δ(8)-sterols.the major sterols of the seeds ofbenincasa cerifera, cucumis sativus, cucurbita maxima, c. pepo andtrichosanthes japonica and of the mature plant tissues (leaves and stems) ofcitrullus battich, cucumis sativus andgynostemma pentaphyllum of the family cucurbitaceae were 24-ethyl-δ(7)-sterols which were accompanied by small amounts of saturated and δ(5)-and δ(8)-sterols. the 24-ethyl-δ(7,22),δ(7,25(27)) and δ(7,22,25(27))-sterols constituted the predominant sterols for the seed materials, whereas ...198627519240
can adverse effects of acidity and aluminum toxicity be alleviated by appropriate rootstock selection in cucumber?low-ph and aluminum (al) stresses are the major constraints that limit crop yield in acidic soils. grafting vegetable elite cultivars onto appropriate rootstocks may represent an effective tool to improve crop tolerance to acidity and al toxicity. two greenhouse hydroponic experiments were performed to evaluate growth, yield, biomass production, chlorophyll index, electrolyte leakage, mineral composition, and assimilate partitioning in plant tissues of cucumber plants (cucumis sativus l. "ekron" ...201627621740
how do modern pesticide treatments influence the mobility of old incurred ddt contaminations in agricultural soils?even more than 50 years after the ban of ddt in germany, farmers are still affected by its persistence in contaminated soils. depending on the crop cultivated on such soils, this often leads to low-level residues of ddt and its metabolites dde and ddd ("ddx"), which are perceived as a risk by the food value chain. pesticide formulations used in modern agriculture commonly contain high levels of surfactants, but so far no open-field studies have evaluated the effects of these treatments on the mo ...201627662464
comparative analysis of cucurbita pepo metabolism throughout fruit development in acorn squash and oilseed pumpkin.both the fruit mesocarp and the seeds of winter squash can be used for consumption, although the focus of breeding efforts varies by cultivar. cultivars bred for fruit consumption are selected for fruit mesocarp quality traits such as carotenoid content, percent dry matter, and percent soluble solids, while these traits are essentially ignored in oilseed pumpkins. to compare fruit development in these two types of squash, we sequenced the fruit transcriptome of two cultivars bred for different p ...201627688889
isolation of protein storage vacuoles and their membranes.protein-storage vacuoles (psvs) are specialized vacuoles that sequester large amounts of storage proteins. during seed development, psvs are formed de novo and/or from preexisting lytic vacuoles. seed psvs can be subdivided into four distinct compartments: membrane, globoid, matrix, and crystalloid. in this chapter, we introduce easy methods for isolation of psvs and their membranes from pumpkin seeds. these methods facilitate the identification and characterization of psv components.201727730610
the introduction of submitted approaches in iranian traditional medicine for treatment of diabetes.diabetes mellitus is a complex disorder of carbohydrate metabolism. according to past studies, the word ziabites in iranian traditional medicine (itm) correlate with diabetes. the aim of this study was to identify the definition, therapeutics, and mechanism of diabetes from the resources of itm in order to provide new recommendations for the treatment of diabetes.201627840534
wheat bread with pumpkin (cucurbita maxima l.) pulp
as a functional food product.in this study, a new application of pumpkin pulp in bread production is shown. the aim of this work is to determine the influence of the addition of fresh pumpkin pulp directly into wheat flour on physical, sensorial and biological properties of bread. the bioaccessibility of active compounds was also studied. an increase in the addition of pumpkin pulp from 5 to 20% (converted to dry matter) caused a decrease of bread volume and increase of crumb hardness and cohesiveness. the sensory character ...201427904316
no accumulation alleviates h2 o2 -dependent oxidative damage induced by ca(no3 )2 stress in the leaves of pumpkin-grafted cucumber seedlings.nitric oxide (no) and hydrogen peroxide (h2 o2 ), two important signaling molecules, are stimulated in plants by abiotic stresses. in this study, we investigated the role of no and its interplay with h2 o2 in the response of self-grafted (s-g) and salt-tolerant pumpkin-grafted (cucurbita maxima × c. moschata) cucumber seedlings to 80 mm ca(no3 )2 stress. endogenous no and h2 o2 production in s-g seedlings increased in a time-dependent manner, reaching maximum levels after 24 h of ca(no3 )2 stres ...201627935073
α-carotene and β-carotene content in raw and cooked pulp of three mature stage winter squash "type butternut".winter squash "type butternut" is harvested in physiological ripening for better commercial distribution, when sensory and/or nutritional quality is not optimum for consumption. the objective of this study was to quantify the content of α-carotene, β-carotene, color and dry matter in the pulp of raw and microwave-cooked winter squash "type butternut" (variety cosmof1) in three states of commercial maturity. immature, mature, and very mature fruit, defined at the time of the harvest by the percen ...201528231218
dechlorination and chlorine rearrangement of 1,2,5,5,6,9,10-heptachlorodecane mediated by the whole pumpkin seedlings.short chain chlorinated paraffins (sccps) are ubiquitously present as persistent organic pollutants in the environment. however, little information on the interaction of sccps with plants is currently available. in this work, young pumpkin plants (cucurbita maxima × c. moschata) were hydroponically exposed to the congener of chlorinated decane, 1,2,5,5,6,9,10-heptachlorodecane (1,2,5,5,6,9,10-hepcd), to investigate the uptake, translocation and transformation of chlorinated decanes in the intact ...201728242253
the development of phloem anastomoses between vascular bundles and their role in xylem regeneration after wounding in cucurbita and dahlia.the differentiation of phloem anastomoses linking the longitudinal vascular bundles has been studied in stem internodes of cucurbita maxima duchesne, c. pepo l. and dahlia pinnata cav. these anastomoses comprise naturally occurring regenerative sieve tubes which redifferentiate from interfascicular parenchyma cells in the young internodes. in all three species, severing a vascular bundle in a young internode resulted in regeneration of xylem to form a curved by-pass immediately around the wound. ...199628321670
fruit quality of seedless watermelon grafted onto squash rootstocks under different production systems.the market demand for seedless watermelon has been continuously increasing because of consumer preference. grafting is a useful tool to manage soil-borne diseases in watermelon production, but the use of squash rootstocks may negatively affect watermelon fruit quality. currently, most research has focused on seeded cultivars while grafting effects on seedless watermelons remain largely unknown. this multi-season study was conducted to assess the effects of squash rootstocks, including both cucur ...201728369915
glucose content and in vitro bioaccessibility in sweet potato and winter squash varieties during storage.glucose content and in vitro bioaccessibility were determined in raw and cooked pulp of arapey, cuabé, and beauregard sweet potato varieties, as well as maravilla del mercado and atlas winter squash, after zero, two, four, and six months of storage (14 °c, 80% relative humidity (rh)). the total glucose content in 100 g of raw pulp was, for arapey, 17.7 g; beauregard, 13.2 g; cuabé, 12.6 g; atlas, 4.0 g; and in maravilla del mercado, 4.1 g. these contents were reduced by cooking process and stora ...201728665302
the bionomics of the cocoa mealybug, exallomochlus hispidus (morrison) (hemiptera: pseudococcidae), on mangosteen fruit and three alternative hosts.the cocoa mealybug, exallomochlus hispidus morrison (hemiptera: pseudococcidae) is known to attack mangosteen, an important fruit export commodity for indonesia. the mealybug is polyphagous, so alternative host plants can serve as a source of nourishment. this study aimed to record the bionomics of e. hispidus on mangosteen (garcinia mangostana l.) and three alternative hosts, kabocha squash (cucurbita maxima l.), soursop (annona muricata, l.), and guava (psidium guajava l.). first-instar nymphs ...201728757558
detection of new viruses in alfalfa, weeds and cultivated plants growing adjacent to alfalfa fields in saudi arabia.a total of 1368 symptomatic plant samples showing different virus-like symptoms such as mottling, chlorosis, mosaic, yellow mosaic, vein clearing and stunting were collected from alfalfa, weed and cultivated plant species growing in vicinity of alfalfa fields in five principal regions of alfalfa production in saudi arabia. das-elisa test indicated occurrence of 11 different viruses in these samples, 10 of which were detected for the first time in saudi arabia. eighty percent of the alfalfa sampl ...201728855829
resurgence of cucurbit downy mildew in the united states: insights from comparative genomic analysis of pseudoperonospora cubensis.pseudoperonospora cubensis, the causal agent of cucurbit downy mildew (cdm), is known to exhibit host specialization. the virulence of different isolates of the pathogen can be classified into pathotypes based on their compatibility with a differential set composed of specific cucurbit host types. however, the genetic basis of host specialization within p. cubensis is not yet known. total genomic dna extracted from nine isolates of p. cubensis collected from 2008 to 2013 from diverse cucurbit ho ...201728861228
karyotype stability and unbiased fractionation in the paleo-allotetraploid cucurbita genomes.the cucurbita genus contains several economically important species in the cucurbitaceae family. here, we report high-quality genome sequences of c. maxima and c. moschata and provide evidence supporting an allotetraploidization event in cucurbita. we are able to partition the genome into two homoeologous subgenomes based on different genetic distances to melon, cucumber, and watermelon in the benincaseae tribe. we estimate that the two diploid progenitors successively diverged from benincaseae ...201728917590
evaluation of three pumpkin species: correlation with physicochemical, antioxidant properties and classification using spme-gc-ms and e-nose methods.to ascertain the most discriminant variables for three pumpkin species principal component analysis (pca) was performed. twenty-four parameters (ph, conductivity, sucrose, glucose, total soluble solids, l*, a*, b*, individual weight, edible rate, firmness, citric acid, fumaric acid, l-ascorbic acid, malic acid, ppo activity, pod activity, total flavonoids, vitamin e, total phenolics, dpph, frap, β-carotene, and aroma) were considered. the studied pumpkin species were cucurbita maxima, cucurbita ...201728974797
evidence based study of antidiabetic potential of c. maxima seeds - in vivo.in vitro antidiabetic efficacy of cucurbita maxima seed extract (cmse) has already been studied in our previous findings. thus, in order to validate these findings in biological system, in vivo antidiabetic activity of aqueous extract was investigated in normal as well as diabetic experimental models.201729034194
anti obese potential of cucurbita maxima seeds oil: effect on lipid profile and histoarchitecture in high fat diet induced obese rats.in this study, we made an attempt to evaluate the potential of cucurbita maxima seeds oil (cso) against high-fat diet (hfd)-induced obesity in rats. we investigated the effect of cso (100 mg/kg body weight) supplementation over 30 days on the changes of hfd-induced obese rats in body weight, biochemical parameters and lipid profile as well as investigated the effects of cso on the histopathological changes. oral administration with cso revealed significant diminution in body weight gain, glucose ...201729047298
identification and genetic characterization of ralstonia solanacearum species complex isolates from cucurbita maxima in china.ralstonia solanacearum species complex is a devastating phytopathogen with an unusually wide host range, and new host plants are continuously being discovered. in june 2016, a new bacterial wilt on cucurbita maxima was observed in guangdong province, china. initially, in the adult plant stage, several leaves of each plant withered suddenly and drooped; the plant then wilted completely, and the color of their vasculature changed to dark brown, ultimately causing the entire plant to die. creamy-wh ...201729093727
coccinia indica agglutinin, a 17kda pp2 like phloem lectin: affinity purification, primary structure and formation of self-assembled filaments.phloem protein-2 (pp2) is an abundant soluble protein in the sieve elements in plants. its lectin property was reported in various species. the primary structure of a 17kda pp2 from coccinia indica (coccinia indica agglutinin, cia17), determined by mass spectrometry, shows extensive homology with pp2 super family phloem lectins. analysis of mass spectrometric data indicated the presence of 16 potential allelic variants of cia17 with insignificant divergence in the primary structure. the primary ...201829126937
salinity tolerance of grafted watermelon seedlings.in order to evaluate the salinity tolerance of grafted watermelon, two sets of experiments were conducted in a growing chamber where 'esmeralda' varieties were grafted onto interspecific squash (cucurbita maxima duch. × cucurbita moschata duch.) and lagenaria siceraria rootstocks. both non-grafted and self-garfted plants were used for control. for salt stress, 2.85 and 4.28 mm/l substrate doses of nacl were added with each irrigation in 2 day intervals for a duration of 23 days. interspecific-gr ...201729262705
uptake, translocation and biotransformation of n-ethyl perfluorooctanesulfonamide (n-etfosa) by hydroponically grown plants.n-ethyl perfluorooctane sulfonamide (n-etfosa) is an important perfluorooctanesulfonate (pfos) precursor (prefos) which is used in sulfluramid. the present work studied the uptake, translocation and metabolism of n-etfosa in wheat (triticum aestivum l.), soybean (glycine max l. merrill) and pumpkin (cucurbita maxima l.) by hydroponic exposure. except for parent n-etfosa, its metabolites of perfluorooctane sulfonamide acetate (fosaa), perfluorooctane sulfonamide (pfosa), pfos, perfluorohexane sul ...201829306808
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