Publications

sieve tube geometry in relation to phloem flow.	sieve elements are one of the least understood cell types in plants. translocation velocities and volume flow to supply sinks with photoassimilates greatly depend on the geometry of the microfluidic sieve tube system and especially on the anatomy of sieve plates and sieve plate pores. several models for phloem translocation have been developed, but appropriate data on the geometry of pores, plates, sieve elements, and flow parameters are lacking. we developed a method to clear cells from cytopla ...	2010	20354199
remote-controlled stop of phloem mass flow by biphasic occlusion in cucurbita maxima.	the relationships between damage-induced electropotential waves (epws), sieve tube occlusion, and stop of mass flow were investigated in intact cucurbita maxima plants. after burning leaf tips, epws propagating along the phloem of the main vein were recorded by extra- and intracellular microelectrodes. the respective epw profiles (a steep hyperpolarization/depolarization peak followed by a prolonged hyperpolarization/depolarization) probably reflect merged action and variation potentials. a few ...	2010	20584788
response of epilachna paenulata to two flavonoids, pinocembrin and quercetin, in a comparative study.	we examined the effects of the flavonoids pinocembrin and quercetin on the feeding behavior, survival, and development of the cucurbitaceae pest epilachna paenulata (coleoptera: coccinellidae). in no-choice experiments, 48 hr-consumption of cucurbita maxima duch. leaves treated with pinocembrin at 1, 5, and 50 microg/cm(2) was less than one third of that for leaves treated with 0.1 microg/cm(2) of pinocembrin or untreated leaves. larvae stopped feeding after 9 days of high doses of pinocembrin ( ...	2010	20589417
differential spatial expression of a- and b-type cdks, and distribution of auxins and cytokinins in the open transverse root apical meristem of cucurbita maxima.	aside from those on arabidopsis, very few studies have focused on spatial expression of cyclin-dependent kinases (cdks) in root apical meristems (rams), and, indeed, none has been undertaken for open meristems. the extent of interfacing between cell cycle genes and plant growth regulators is also an increasingly important issue in plant cell cycle studies. here spatial expression/localization of an a-type and b-type cdk, auxin and cytokinins are reported in relation to the hitherto unexplored an ...	2011	20601387
carotenoid content and in vitro bioaccessibility of lutein in some leafy vegetables popular in sri lanka.	lutein is one of the major carotenoids in green leafy vegetables (glvs), which show a marked antioxidant activity. the study was carried out to identify and quantify the carotenoid content of selected sri lankan glvs. rp-hplc with photodiode array detection method was followed for the separation, identification and quantification of carotenoids. among the vegetables analyzed, ipomoea batatas leaves showed the highest beta-carotene content (743.9+/-35.0 microg/g dry weight (dw)) while syngonium a ...	2010	20651462
sodium and chloride exclusion and retention by non-grafted and grafted melon and cucurbita plants.	the effects of grafting on na and cl(-) uptake and distribution in plant tissues were quantified in a greenhouse experiment using six combinations of melon (cucumis melo l. cv. arava) and pumpkin (cucurbita maxima duchesne×cucurbita moschata duchesne cv. tz-148): non-grafted, self-grafted, melons grafted on pumpkins, and pumpkins grafted on melons. total na concentration in shoots of plants with pumpkin or melon rootstocks was <60 mmol kg(-1) and >400 mmol kg(-1), respectively, regardless of the ...	2011	20729482
assessing risk to human health from tropical leafy vegetables grown on contaminated urban soils.	fifteen tropical leafy vegetable types were sampled from farmers' gardens situated on nine contaminated sites used to grow vegetables for commercial or subsistence consumption in and around kampala city, uganda. trace metal concentrations in soils were highly variable and originated from irrigation with wastewater, effluent discharge from industry and dumping of solid waste. metal concentrations in the edible shoots of vegetables also differed greatly between, and within, sites. gynandropsis gyn ...	2010	20739044
pumpkin eif5a isoforms interact with components of the translational machinery in the cucurbit sieve tube system.	in yeast, eif5a, in combination with eef2, functions at the translation step, during the protein elongation cycle. this result is of significance with respect to functioning of the enucleate sieve tube system, as eif5a was recently detected in cucurbita maxima (pumpkin) phloem sap. in the present study, we further characterized four cmeif5a isoforms, encoding three proteins, all of which were present in the phloem sap. although hypusination of cmeif5a was not necessary for entry into the sieve e ...	2010	20807213
a mottled-leaf character in winter squash; inherited as a dominant mendelian character.		1946	21015950
agrobacterium-mediated transformation of kabocha squash (cucurbita moschata duch) induced by wounding with aluminum borate whiskers.	an efficient genetic transformation method for kabocha squash (cucurbita moschata duch cv. heiankogiku) was established by wounding cotyledonary node explants with aluminum borate whiskers prior to inoculation with agrobacterium. adventitious shoots were induced from only the proximal regions of the cotyledonary nodes and were most efficiently induced on murashige-skoog agar medium with 1 mg/l benzyladenine. vortexing with 1% (w/v) aluminum borate whiskers significantly increased agrobacterium i ...	2011	21400224
isothermal titration calorimetric and computational studies on the binding of chitooligosaccharides to pumpkin (cucurbita maxima) phloem exudate lectin.	the interaction of chitooligosaccharides [(glcnac)(2-6)] with pumpkin phloem exudate lectin (ppl) was investigated by isothermal titration calorimetry and computational methods. the dimeric ppl binds to (glcnac)(3-5) with binding constants of 1.26-1.53 × 10(5) m(-1) at 25 °c, whereas chitobiose exhibits approximately 66-fold lower affinity. interestingly, chitohexaose shows nearly 40-fold higher affinity than chitopentaose with a binding constant of 6.16 × 10(6) m(-1). the binding stoichiometry ...	2011	21405077
vascular expression in arabidopsis is predicted by the frequency of ct/ga-rich repeats in gene promoters.	phloem-transported signals play an important role in regulating plant development and in orchestrating responses to environmental stimuli. among such signals, phloem-mobile rnas have been shown to play an important role as long-distance signaling agents. at maturity, angiosperm sieve elements are enucleate, and thus transcripts in the phloem translocation stream probably originate from the nucleate companion cells. in the present study, a pumpkin (cucurbita maxima) phloem transcriptome was used ...	2011	21435051
cmrbp50 protein phosphorylation is essential for assembly of a stable phloem-mobile high-affinity ribonucleoprotein complex.	rna-binding proteins (rbps) form ribonucleoprotein (rnp) complexes that play crucial roles in rna processing for gene regulation. the angiosperm sieve tube system contains a unique population of transcripts, some of which function as long-distance signaling agents involved in regulating organ development. these phloem-mobile mrnas are translocated as rnp complexes. one such complex is based on a phloem rbp named cucurbita maxima rna-binding protein 50 (cmrbp50), a member of the polypyrimidine tr ...	2011	21572046
effects of cooking on the cell walls (dietary fiber) of 'scarlet warren' winter squash ( cucurbita maxima ) studied by polysaccharide linkage analysis and solid-state (13)c nmr.	cell wall polysaccharides of 'scarlet warren' winter squash ( cucurbita maxima ) were investigated before and after thermal processing. linkage analysis of polysaccharides was done by gas chromatography coupled to mass spectrometry (gc-ms). the linkage analysis showed the cell wall polysaccharide compositions of raw and cooked squash were similar. the total pectic polysaccharides (galacturonan, rhamnogalacturonan, arabinan, and arabinogalactan) contents of the cell walls of both raw and cooked s ...	2011	21604813
an in vitro evaluation of anthelmintic activity of zingiber zerumbet rhizomes and cucurbita maxima seeds on pheretima posthuma model: a comparative study.		2011	21687369
comparison of waste pumpkin material and its potential use in extruded snack foods.	material was produced from crown pumpkin (cucurbita maxima) processed from fractions of the fruit which are regarded as waste stream products (peel, flesh and seed). the flour from the three different fractions (peel, flesh and seed) of crown pumpkin flour was incorporated into an extruded snack product formulation at levels 10%, 30% and 50% (w/w with corn grit) and processed in a twin-screw extruder to make 10 expanded snack products. proximate analysis was carried out to determine the nutritio ...	2011	21813596
random estimate the values of seed oil of cucurbita maxima by refractive index method.	the crude oil having lower iodine and free fatty acids values has aamdosha properties. these properties are present due to toxic and anti-toxic compounds. these compounds can be harmful for the special diseases and may be unsaturated, saturated, open chain etc. the adulteration can take part as catalytic action for the toxic effect for the special diseases. toxic properties of oils are removed by different ingrediants and methods. c. maxima seed tail (mst) is used with food and medicine. the pre ...	2010	22131677
quantification of plant cell coupling with three-dimensional photoactivation microscopy.	plant cells are directly connected by plasmodesmata that form channels through the cell wall and enable the intercellular movement of cytosolic solutes, membrane lipids and signalling molecules. transport through plasmodesmata is regulated not only by a fixed size-exclusion limit, but also by physiological and pathological adaptation. to understand plant cell communication, carbon allocation and pathogen attack, the capacities for a specific molecule to pass a specific cell-wall interface is an ...	2012	22171617
buttercup squash provides a marketable alternative to blue hubbard as a trap crop for control of striped cucumber beetles (coleoptera: chrysomelidae).	winter squash is a vital agricultural commodity worldwide. in the northeastern united states, the primary insect pest is the striped cucumber beetle, acalymma vittatum f. using a blue hubbard squash (cucurbita maxima duchesne) perimeter trap crop system can reduce insecticide use by >90% in butternut squash (c. moschata poir), the primary winter squash grown in this region. despite the savings in insecticide costs, growers may be reluctant to give up field space for a perimeter crop of blue hubb ...	2010	22182562
accumulation of weathered p,p'-ddts in grafted watermelon.	the grafting of melon plants onto cucurbit rootstocks is a common commercial practice in many parts of the world. however, certain cucurbits have been shown to accumulate large quantities of weathered persistent organic pollutants from the soil, and the potential contamination of grafted produce has not been thoroughly evaluated. large pot and field experiments were conducted to assess the effect of grafting on accumulation of weathered ddx (the sum of p,p'-ddt, p,p'-ddd, and p,p'-dde) from soil ...	2012	22224752
content of bioactive compounds and antioxidant capacity of pumpkin puree enriched with japanese quince, cornelian cherry, strawberry and apples.	when evaluated in terms of taste, smell or active ingredients, pumpkin in itself is not very attractive as a raw material. hence, it is recommended to blend pumpkin with other fruits, which are aromatic, have a defined taste, and contain a large quantity of active ingredients and organic acids to improve its palatibility.	2013	22232528
stamen-derived bioactive gibberellin is essential for male flower development of cucurbita maxima l.	gibberellin (ga) signalling during pumpkin male flower development is highly regulated, including biosynthetic, perception, and transduction pathways. ga 20-oxidases, 3-oxidases, and 2-oxidases catalyse the final part of ga synthesis. additionally, 7-oxidase initiates this part of the pathway in some cucurbits including cucurbita maxima l. (pumpkin). expression patterns for these ga-oxidase-encoding genes were examined by competitive reverse transcription-pcr (rt-pcr) and endogenous ga levels we ...	2012	22268154
the origin and composition of cucurbit "phloem" exudate.	cucurbits exude profusely when stems or petioles are cut. we conducted studies on pumpkin (cucurbita maxima) and cucumber (cucumis sativus) to determine the origin and composition of the exudate. morphometric analysis indicated that the exudate is too voluminous to derive exclusively from the phloem. cold, which inhibits phloem transport, did not interfere with exudation. however, ice water applied to the roots, which reduces root pressure, rapidly diminished exudation rate. sap was seen by micr ...	2012	22331409
in vivo quantification of cell coupling in plants with different phloem-loading strategies.	uptake of photoassimilates into the leaf phloem is the key step in carbon partitioning and phloem transport. symplasmic and apoplasmic loading strategies have been defined in different plant taxa based on the abundance of plasmodesmata between mesophyll and phloem. for apoplasmic loading to occur, an absence of plasmodesmata is a sufficient but not a necessary criterion, as passage of molecules through plasmodesmata might well be blocked or restricted. here, we present a noninvasive, whole-plant ...	2012	22422939
looking deep inside: detection of low-abundance proteins in leaf extracts of arabidopsis and phloem exudates of pumpkin.	the field of proteomics suffers from the immense complexity of even small proteomes and the enormous dynamic range of protein concentrations within a given sample. most protein samples contain a few major proteins, which hamper in-depth proteomic analysis. in the human field, combinatorial hexapeptide ligand libraries (cpll; such as proteominer) have been used for reduction of the dynamic range of protein concentrations; however, this technique is not established in plant research. in this work, ...	2012	22555880
transgenic tobacco plants as production platform for biologically active human interleukin 2 and its fusion with proteinase inhibitors.	transgenic plants offer a low-cost approach for the production of pharmaceutically important and commercially valuable recombinant proteins. our studies were focused on the plant-based production of human interleukin 2 (hil-2) and its fusion with proteinase inhibitors, either spi2 from galleria mellonella or cmti from cucurbita maxima. finally, five plant expression cassettes were obtained. three of them contained the single cdna encoding cmti i, spi2 and hil-2, respectively, while two of them c ...	2012	22564275
carotenoid accumulation and carotenogenic gene expression during fruit development in novel interspecific inbred squash lines and their parents.	carotenoid levels and composition during squash fruit development were compared in cucurbita moschata , cucurbita maxima , and two lines of their interspecific inbred lines, namely, maxchata1 and maxchata2. eight genes associated with carotenoid biosynthesis were analyzed by quantitative rt-pcr. the two squash species and their interspecific inbred lines exhibited different qualitative and quantitative carotenoid profiles and regulatory mechanisms. c. moschata had the lowest total carotenoid con ...	2012	22574777
comparative analysis of cells and proteins of pumpkin plants for the control of fruit size.	common pumpkin plants (cucurbita maxima) produce fruits of 1-2 kg size on the average, while special varieties of the same species called atlantic giant are known to produce a huge fruit up to several hundred kilograms. as an approach to determine the factors controlling the fruit size in c. maxima, we cultivated both ag and control common plants, and found that both the cell number and cell sizes were increased in a large fruit while dna content of the cell did not change significantly. we also ...	2012	22677066
sieve element occlusion (seo) genes encode structural phloem proteins involved in wound sealing of the phloem.	the sieve element occlusion (seo) gene family originally was delimited to genes encoding structural components of forisomes, which are specialized crystalloid phloem proteins found solely in the fabaceae. more recently, seo genes discovered in various non-fabaceae plants were proposed to encode the common phloem proteins (p-proteins) that plug sieve plates after wounding. we carried out a comprehensive characterization of two tobacco (nicotiana tabacum) seo genes (ntseo). reporter genes controll ...	2012	22733783
interaction of xylem and phloem during exudation and wound occlusion in cucurbita maxima.	collection of cucurbit exudates from cut petioles has been a powerful tool for gaining knowledge on phloem sap composition without full notion of the complex exudation mechanism. only few publications explicitly mentioned that exudates were collected from the basal side of the cut, which exudes more copiously than the apical side. this is surprising since only exudation from the apical side is supposedly driven by phloem pressure gradients. composition of carbohydrates and ph values at both woun ...	2013	22765252
high efficiency joint cze determination of sugars and acids in vegetables and fruits.	in this work, an improved ce method for the medium-throughput determination of main organic acids (oxalate, malate, citrate), the amino acid glutamate and the sugars fructose, glucose and sucrose in several food matrices is described. these compounds have been identified as key components in the taste intensity of fruit and vegetable crops. using a running buffer with 20 mm 2,6-pyridine dicarboxylic acid ph 12.1 and 0.1% hexadimethrine bromide, replacing it every 5 h to avoid ph decrease, and op ...	2012	22887163
[salt stress tolerance of cucumber-grafted rootstocks].	taking 4 different cucurbita maxima x c. moschata rootstocks for cucumber (cucumis sativus) as test materials, a solution culture experiment was conducted to study their growth and antioxidative enzyme activities under the stresses of ca(no3)2 and nacl, with the salt stress tolerance of the rootstocks evaluated by subordinate function. at 30 mmol x l(-1) of ca (no3)2 or 45 mmol x l(-1) of nacl, the growth of the rootstock seedlings was improved; but at 60 and 120 mmol x l(-1) of ca(no3)2 or 90 a ...	2012	22919842
overexpression of arabidopsis plasmodesmata germin-like proteins disrupts root growth and development.	in plants, a population of non-cell-autonomous proteins (ncaps), including numerous transcription factors, move cell to cell through plasmodesmata (pd). in many cases, the intercellular trafficking of these ncaps is regulated by their interaction with specific pd components. to gain further insight into the functions of this ncap pathway, coimmunoprecipitation experiments were performed on a tobacco (nicotiana tabacum) plasmodesmal-enriched cell wall protein preparation using as bait the ncap, p ...	2012	22960910
deciphering systemic wound responses of the pumpkin extrafascicular phloem by metabolomics and stable isotope-coded protein labeling.	in cucurbits, phloem latex exudes from cut sieve tubes of the extrafascicular phloem (efp), serving in defense against herbivores. we analyzed inducible defense mechanisms in the efp of pumpkin (cucurbita maxima) after leaf damage. as an early systemic response, wounding elicited transient accumulation of jasmonates and a decrease in exudation probably due to partial sieve tube occlusion by callose. the energy status of the efp was enhanced as indicated by increased levels of atp, phosphate, and ...	2012	23085839
dissoconiaceae associated with sooty blotch and flyspeck on fruits in china and the united states.	zasmidium angulare, a novel species of mycosphaerellaceae, and several novel taxa that reside in dissoconiaceae, were identified from a collection of apples and cucurbita maxima (cv. blue hubbard) from china and the usa that exhibited sooty blotch and flyspeck (sbfs) signs on their host substrata. morphology on fruit surfaces and in culture, and phylogenetic analyses of the nuclear ribosomal dnas 28s and internal transcribed spacer regions, as well as partial translation elongation factor 1-alph ...	2012	23105157
microplot evaluation of rootstocks for control of meloidogyne incognita on grafted tomato, muskmelon, and watermelon.	microplot experiments were conducted over two years (four growing seasons) to evaluate meloidogyne incognita resistance in rootstocks used for grafted tomato (solanum lycopersicum), muskmelon (cucumis melo), and watermelon (citrullus lanatus). three tomato rootstocks; 'tx301', 'multifort', and 'aloha', were tested in addition to the nongrafted scion, 'florida-47'. two muskmelon rootstocks; cucumis metuliferus and 'tetsukabuto' (cucurbita maxima × cucurbita moschata) were evaluated with the nongr ...	2011	23431109
in vivo metabolism of 2,2',4,4'-tetrabromodiphenyl ether (bde-47) in young whole pumpkin plant.	polybrominated diphenyl ethers (pbdes) are widely distributed persistent organic pollutants. in vitro and in vivo research using various animal models have shown that pbdes might be transformed to hydroxylated pbdes, but there are few studies on in vivo metabolism of pbdes by intact whole plants. in this research, pumpkin plants (cucurbita maxima × c. moschata) were hydroponically exposed to 2,2',4,4'-tetrabromodiphenyl ether (bde-47). a debromination product (bde-28) and four hydroxylated metab ...	2013	23510101
characteristics of antioxidant activity and composition of pumpkin seed oils in 12 cultivars.	the objective of this study was to determine the antioxidant properties, and provide characteristics, of the oil obtained from the seeds of 12 pumpkin varieties belonging to the species cucurbita maxima duch. and cucurbita pepo l. another objective was to establish which of the two extracting agents, ethanol or methanol, is more effective. the seeds of the pumpkin varieties examined differ in chemical composition and antioxidant activity. the seeds of the cultivars belonging to the species c. ma ...	2013	23561092
three new multiflorane-type triterpenes from pumpkin (cucurbita maxima) seeds.	three new multiflorane-type triterpenes; 7a-methoxymultiflor-8-ene-3a,29-diol 3-acetate-29-benzoate (1), 7-oxomultiflor-8-ene-3a,29-diol 3-acetate-29-benzoate (2), and multiflora-7,9(11)-diene-3a,29-diol 3-p-hydroxybenzoate-29-benzoate (3), were isolated from seeds of cucurbita maxima, along with three known compounds. compound 3 and multiflora-7,9(11)-diene-3a-29-diol 3-benzoate (5) exhibited potent inhibitory effects on melanogenesis, with low cytotoxicities, and 2 exhibited single-digit micro ...	2013	23673529
pumpkin (cucurbita maxima) seed proteins: sequential extraction processing and fraction characterization.	seed proteins extracted from tunisian pumpkin seeds ( cucurbita maxima ) were investigated for their solubility properties and sequentially extracted according to the osborne procedure. the solubility of pumpkin proteins from seed flour was greatly influenced by ph changes and ionic strength, with higher values in the alkaline ph regions. it also depends on the seed defatting solvent. protein solubility was decreased by using chloroform/methanol (cm) for lipid extraction instead of pentane (p). ...	2013	23869935
characterization of the pumpkin translationally-controlled tumor protein cmtctp.	in higher plants, the phloem plays a central role in the delivery of nutrients and signals from source to sink tissues. these signals likely coordinate different aspects of plant development, as well as its response to environmental cues. although some phloem-transported proteins and rnas may function as signaling molecules in plants, their mode of action remains poorly understood. previous analysis of transcripts from cmv-infected pumpkin (cucurbita maxima cv big max) identified a translational ...	2013	24065051
biosynthesis of 12α-and 13-hydroxylated gibberellins in a cell-free system from cucurbita maxima endosperm and the identification of new endogenous gibberellins.	gibberellin (ga) biosynthesis in cell-free systems from cucurbita maxima l. endosperm was reinvestigated using incubation conditions different from those employed in previous work. the metabolism of ga12 yielded ga13, ga43 and 12α-hydroxyga43 as major products, ga4, ga37, ga39, ga46 and four unidentified compounds as minor products. the intermediates ga15, ga24 and ga25 accumulated at low protein concentrations. the structure of the previously uncharacterised 12α-hydroxyga43 was inferred from it ...	1993	24178490
gibberellin biosynthesis in cell-free extracts from developing cucurbita maxima embryos and the identification of new endogenous gibberellins.	gibberellin (ga) biosynthetic pathways from ga12-aldehyde, ga12 and ga53 were investigated in cell-free systems from developing embryos of cucurbita maxima l. gibberellin a12-aldehyde and ga12 were converted to ga25, putative 12α-hydroxyga25, ga13 and ga39 as main products. minor products were ga4, ga34 and, when ga12 was the substrate, putative 12α-hydroxyga12. the intermediates ga15 and ga24 accumulated at low protein concentrations. the influence of various factors on ga12 metabolism was exam ...	1993	24178491
cloning of a cdna encoding 1-aminocyclopropane-1-carboxylate synthase and expression of its mrna in ripening apple fruit.	1-aminocyclopropane-1-carboxylate (acc) synthase (ec 4.4.1.14) purified from apple (malus sylvestris mill.) fruit was subjected to trypsin digestion. following separation by reversed-phase high-pressure liquid chromatography, ten tryptic peptides were sequenced. based on the sequences of three tryptic peptides, three sets of mixed oligonucleotide probes were synthesized and used to screen a plasmid cdna library prepared from poly(a)(+) rna of ripe apple fruit. a 1.5-kb (kilobase) cdna clone whic ...	1991	24186277
metabolites of 2,4,4'-tribrominated diphenyl ether (bde-28) in pumpkin after in vivo and in vitro exposure.	there is currently limited knowledge on pbde metabolism in plants although they could play an important role in the environmental transformation of these persistent organic pollutants. in this study, pumpkin (cucurbita maxima × c. moschata) was chosen as the model to understand the fate of bde-28 in plants. meo-tri-bdes, oh-tri-bdes, and oh-tri-bdes were found as metabolites in plant samples of both in vivo hydroponic and in vitro tissue culture exposure. three meo-tri-bdes were further identifi ...	2013	24191731
microwave-assisted aqueous enzymatic extraction of oil from pumpkin seeds and evaluation of its physicochemical properties, fatty acid compositions and antioxidant activities.	microwave-assisted aqueous enzymatic extraction (maaee) of pumpkin seed oil was performed in this study. an enzyme cocktail comprised of cellulase, pectinase and proteinase (w/w/w) was found to be the most effective in releasing oils. the highest oil recovery of 64.17% was achieved under optimal conditions of enzyme concentration (1.4%, w/w), temperature (44°c), time (66 min) and irradiation power (419w). moreover, there were no significant variations in physicochemical properties of maaee-extra ...	2014	24206680
iron assimilation in plants: reduction of a ferriphytosiderophore by nadh:nitrate reductase from squash.	nadh:nitrate reductase (ec 1.6.6.1) from squash (cucurbita maxima duch., cv. buttercup) can catalyze the reduction of a ferriphytosiderophore from barley (hordeum vulgare l. cv. europa). maximal activity occurs at ph 6, with an apparentk m andv max of 76 μm and 21 nmol·min(-1)·(mg protein)(-1), respectively. the ferriphytosiderophore strongly inhibits nitrate reduction catalyzed by nitrate reductase at the optimal ph for nitrate reduction, i.e. 7.5. on the contrary, nitrate is a poor inhibitor o ...	1988	24226694
the loss of carbon-20 in c19-gibberellin biosynthesis in a cell-free system from pisum sativum l.	the fate of the carbon-20 atom in gibberellin (ga) biosynthesis was studied in a cell-free system from pisum sativum. this carbon atom is lost at the aldehyde stage of oxidation when c20-gas are converted to c19-gas. gibberellin a12 labeled with (14)c at c-20 was prepared from [3'-(14)c]mevalonic acid with a cell-free system from cucurbita maxima and incubated with the pea system. analysis of the gas and aqueous phases showed that (14)co2 was formed at the same rate and in nearly equivalent amou ...	1986	24232760
transcription and translation of phloem protein (pp2) during phloem differentiation in cucurbita maxima.	the synthesis of a major phloem protein, pp2, was investigated by measurement of the mrna at various stages of phloem development in cucurbita. quantitative assays with immuno-electrophoresis showed that the amounts of pp2 in hypocotyls of cucurbita seedlings increased with the age of seedlings. an increase in mrna for pp2 during the early stages of seedling growth was also observed by immunoprecipitation of the invitro translation products of hypocotyl polyadenylated rna. there was close timing ...	1987	24232970
immunocytochemical localisation of phloem lectin from cucurbita maxima using peroxidase and colloidal-gold labels.	antibodies were raised against lectin purified from the sieve-tube exudate of cucurbita maxima. immunocytochemistry, using peroxidase-labelled antibodies and protein a-colloidal gold, was employed to determine the location of the lectin within the tissues and cells of c. maxima and other cucurbit species. the anti-lectin antibodies bound to p-protein aggregates in sieve elements and companion cells, predominantly in the extrafascicular phloem of c. maxima. this may reflect the low rate of transl ...	1987	24233009
different at-rich satellite dnas in cucurbita pepo and cucurbita maxima.	the at-rich highly repeated satellite dna of cucurbita pepo (zucchini) and cucurbita maxima (pumpkin) were cloned and their dna structure was investigated. dna sequencing revealed that the repeat length of satellite dna in cucurbita pepo is 349-352 base pairs. the percentage of at-base pairs is about 61%. this satellite is highly conserved in restriction enzyme pattern and dna sequence; sequence heterogeneity is about 10%. in contrast, the satellite dna of cucurbita maxima has a repeat length of ...	1986	24240758
conversion of gibberellin a20 to gibberellins a 1 and a 5 in a cell-free system from phaseolus vulgaris.	the soluble fraction of a cell-free system from immature seeds of phaseolus vulgaris l. converts gibberellin a20 (ga20) to ga1 and ga5. it does however not metabolize ga1 and ga29 to ga5, showing that in this system ga20 is converted directly to ga5. the steps from ga20 to ga1 (3-hydroxylation) and from ga20 to ga5 (δ(2) double-bond formation) require oxygen, fe(2+) and α-ketoglutarate, and are stimulated by ascorbate. the enzymes catalyzing these conversions bate. the enzymes catalyzing these c ...	1984	24254050
subcellular localization of h(+)-atpase from pumpkin hypocotyls (cucurbita maxima l.) by membrane fractionation.	a new method of preparing sealed vesicles from membrane fractions of pumpkin hypocotyls in ethanolamine-containing buffers was used to investigate the subcellular localization of h(+)-atpase measured as nigericin-stimulated atpase. in a fluorescence-quench assay, the h(+) pump was directly demonstrated. the h(+) pump was substrate-specific for mg·atp and 0.1 mm diethylstilbestrol completely prevented the development of a δ ph. the presence of unsupecific phosphatase hampered the detection of nig ...	1984	24258584
characteristics of organic acids in the fruit of different pumpkin species.	the aim of the research was to determine the composition of organic acids in fruit of different cultivars of three pumpkin species. the amount of acids immediately after fruit harvest and after 3 months of storage was compared. the content of organic acids in the examined pumpkin cultivars was assayed using the method of high performance liquid chromatography (hplc). three organic acids (citric acid, malic acid, and fumaric acid) were identified in the cultivars, whose content considerably varie ...	2014	24262577
indigenous and traditional plants: south african parents' knowledge, perceptions and uses and their children's sensory acceptance.	the dietary shift from indigenous and traditional plants (itps) to cash crops and exotic plant food sources increases the risk of malnutrition and other nutrition-related non-communicable diseases, especially in poor rural communities. farm communities in south africa have been associated with poor nutritional status and extreme poverty. itps have been found to be affordable sources of several micronutrients. however, knowledge of and the use of these plants are declining, and little is known ab ...	2013	24274394
bioactivities of some essential oils against the camel nasal botfly, cephalopina titillator.	nasopharyngeal myiasis of camels is caused by the larvae of cephalopina titillator. we determined the efficacy of essential oils (eos) of pumpkin, cucurbita maxima; lupinus, lupinus luteus; garlic oil, allium sativum; and peppermint, mentha piperita, against the third larval stage of c. titillator using larval immersion tests. the positive control group was treated with ivermectin and the negative control one was treated with distilled water and few drops of tween 80. larvae were reared until ad ...	2014	24276644
auxin-stimulated atpase in membrane fractions from pumpkin hypocotyls (cucurbita maxima l.).	membrane fractions from cucurbita maxima hypocotyls were isolated in a medium which inhibits the action of endogenous phospholipases. after removal of soluble phosphatases by sepharose 2b-cl column chromatography, an auxin-stimulated atpase activity was found in membrane fractions from linear sucrose gradients. in the presence of 10(-4) m phenylacetic acid (paa), the stimulation by indol-3-acetic acid (iaa) exhibited a bimodal concentration dependence with maximal stimulation of about 50% at 10( ...	1981	24302108
calcium-binding protein in sieve tube exudate.	a calcium-binding macromolecule, with an estimated molecular weight greater than 100,000, was detected in phloem exudate from cucurbita maxima and related species. the macromolecule was a component of sieve tube sap, rather than a contaminant leached from cell walls or cut parenchyma cells during exudate collection. the protein nature of this macromolecule was deduced from its size, lability, susceptibility to proteolytic digestion, and by the dependence of calcium-binding activity on thiol-prot ...	1981	24302204
identification of a volatile attractant for diabrotica and acalymma spp. from blossoms of cucurbita maxima duchesne.	fractionation of headspace volatiles from cucurbita maxima blossoms by high-performance liquid chromatography resulted in the isolation of a single component which was highly active in an electroantennogram bioassay on diabrotica undecimpunctata howardi antennae. this compound was identified as indole by gas chromatography-mass spectrometry. field-trapping bioassays were conducted which indicated that indole is a potent attractant of the western corn rootworm,d. virgifera virgifera, and the stri ...	1986	24306908
crystal structure of korean pine (pinus koraiensis) 7s seed storage protein with copper ligands.	the prevalence of food allergy has increased in recent years, and korean pine vicilin is a potential food allergen. we have previously reported the crystallization of korean pine vicilin purified from raw pine nut. here we report the isolation of vicilin mrna and the crystal structure of korean pine vicilin at 2.40 å resolution. the overall structure of pine nut vicilin is similar to the structures of other 7s seed storage proteins and consists of an n-terminal domain and a c-terminal domain. ea ...	0	24328105
antioxidant enzymatic activities and gene expression associated with heat tolerance in the stems and roots of two cucurbit species ("cucurbita maxima" and "cucurbita moschata") and their interspecific inbred line "maxchata".	the elucidation of heat tolerance mechanisms is required to combat the challenges of global warming. this study aimed to determine the antioxidant enzyme responses to heat stress, at the enzymatic activity and gene expression levels, and to investigate the antioxidative alterations associated with heat tolerance in the stems and roots of squashes using three genotypes differing in heat tolerance. plants of heat-tolerant "c. moschata", thermolabile "c. maxima" and moderately heat-tolerant intersp ...	2013	24336062
direct and individual analysis of stress-related phytohormone dispersion in the vascular system of cucurbita maxima after flagellin 22 treatment.	• the stress-related phytohormones, salicylic acid (sa) and abscisic acid (aba), and the three jasmonates, jasmonic acid (ja), cis-12-oxo-phytodienoic acid (cis-opda), and (+)-7-iso-jasmonoyl-l-isoleucine (ja-ile), were investigated in phloem and xylem exudates of cucurbita maxima. • phloem and xylem exudates were separately collected and analysed via liquid chromatography-mass spectrometry. • we show direct evidence for all three jasmonates, aba, and sa in both phloem and xylem exudates of c. m ...	2014	24387138
the isolation and some properties of a lectin (haemagglutinin) from cucurbita phloem exudate.	the occurrence of high haemagglutinating (lectin) activity in phloem exudate from three cucurbit species is reported. the protein responsible for this lectin activity in cucurbita maxima duch. has been isolated by cation exchange chromatography on sepharose and identified by gel electrophoresis. the lectin showed agglutinating activity at concentrations as low as 0.1 μg/ml. no sugar, including those transported in the phloem of these species, interacted with agglutination. the lectin could not b ...	1978	24408004
peroxidases do not move in phloem.	phloem exudates from grafts between queensland blue pumpkin (cucurbita maxima duch.) and candy red hawkesbury watermelon (citrullus vulgaris schrad.) were analysed by iso-electric focusing to detect iso-enzymes of peroxidase. these enzymes did not move in intact phloem but, when stems were cut, they surged rapidly through graft unions.	1978	24413951
a comparative analysis of phloem exudate proteins from cucumis melo, cucumis sativus and cucurbita maxima by polyacrylamide gel electrophoresis and isoelectric focusing.	proteins in sieve tube exudate from cucumis melo l., cucumis sativus l. and cucurbita maxima duch. were analysed by gel electrophoresis and isoelectric focusing. estimated molecular weights and isoelectric points for the major and minor proteins from each plant species are presented. electrophoresis revealed striking differences between the protein complements of exudatc from the two genera investigated. similarly, although a few exudate proteins from the two species of cucumis possessed identic ...	1976	24424601
the heterogeneity of phloem exudate proteins from different plants: a comparative survey of ten plants using polyacrylamide gel electrophoresis.	proteins in sieve tube exudate from ricinus communis l., acer pseudoplatanus l., aesculus hippocastanum l., cucumis melo l., and two cultivars each of cucumis sativus l., cucurbita pepo l. and cucurbita maxima duchesne were fractionated and compared using polyacrylamide gel electrophoresis. striking differences in major exudate proteins were displayed among the genera and species examined. even cultivars within a single species, although showing general similarities, differed in some prominent p ...	1976	24424985
synthesis of p-protein in mature phloem of cucurbita maxima.	cotyledons of cucurbita maxima duch. seedlings were provided with (14)c-labeled amino acids for 12 h. besides the bulk of labeled amino acids the sieve-tube exudate also carried labeled proteins. 80% of the incorporated radioactivity was found in the p-protein, 20% in a neutral protein, and traces were found in acidic proteins after fractionation on diethyl-aminoethyl cellulose columns. the radioactive elutes were characterized by autoradiographs of both disc- and sodium dodecyl sulfate-gelelect ...	1976	24424987
a re-examination of the reputed control of cotyledonary metabolism by the axis.	a re-examination has been made of the supposed regulation by the axis of cotyledonary isocitrate lyase (ec 4.1.3.1) activity in cucumis sativas l. and cucurbita maxima duch. and greening in cucumis. when precautions are taken to ensure that isolated cotyledons and cotyledons attached to the axis have equal access to oxygen and/or water no axis effects can be observed. the results underline the importance of maintaining excised plant parts in comparable physical conditions if meaningful physiolog ...	1976	24425000
filament formation in vitro of a sieve tube protein from cucurbita maxima and cucurbita pepo.	phloem proteins of the sieve tube exudate from cucurbita maxima duch. and cucurbita pepo l. were investigated as to their filament forming ability in vitro. from the two main proteins (116000 dalton, 30000 dalton) only the 116000 dalton protein was found to form reversibly distinct filaments of 6-7 nm diameter upon removal of sh-protecting agents from the buffer, whereas the 30000 dalton protein was precipitated as amorphous material under these conditions. the protein filaments were similar to ...	1975	24430373
sieve-tube proteins from cucurbita maxima.	the two main proteins from the phloem exudate of cucurbita maxima duchesne have been isolated by ammonium-sulfate precipitation, deae-cellulose chromatography, and gel filtration, and have been characterized. they comprise about 40% each of the total protein. the amino-acid composition of these two proteins has been determined. both are highly basic with an iep above 9.5. the smaller protein has a molecular weight of ca. 30000 as determined by analytical ultracentrifugation, gel filtration, and ...	1974	24442451
the biosynthesis of a c19-gibberellin from mevalonic acid in a cell-free system from a higher plant.	incubation of a cell-free system from immature seeds of cucurbita maxima duch. with [(14)c]ga12-aldehyde derived from [(14)c]mevalonic acid in the same system yielded the c19-gibberellin, [(14)c]ga4, in addition to the c20-gibberellins, [(14)c]ga37, [(14)c]ga13 and [(14)c]ga43. (ga-gibberellin).	1974	24442706
p-protein distribution in mature sieve elements of cucurbita maxima.	portions of the hypocotyls of 16-day-old cucurbita maxima plants, from which the cotyledons and first foliage leaves had been removed 2 days earlier, were fixed in glutaraldehyde and postfixed in osmium tetroxide for electron microscopy. in well over 90% of the mature sieve elements examined the p-protein was entirely parietal in distribution in both the lumina and sieve-plate pores. in addition to the parietal p-protein, the unoccluded sieve-plate pores were lined by narrow callose cylinders an ...	1972	24474154
adenosine triphosphatase in the phloem of cucurbita.	the distribution of adenosine triphosphatase (atpase) activity in the phloem of petioles and minor veins of cucurbita maxima has been studied using a lead phosphate precipitation procedure. atpase activity was localized in sieve elements, companion cells and parenchyma cells. activity was found at the cell surfaces, associated with the dispersed p-protein of mature sieve elements, in mitochondria, sieve-element reticulum, and at specific regions of the cell walls. it is suggested that the atpase ...	1973	24474397
molecular weights of cucurbita sieve tube proteins.	by means of sds-polyacrylamide-gel electrophoresis, molecular weights of 15000, 28000, 59000, 116000 and 220000 were determined for the main sieve tube proteins from cucurbita maxima.	1971	24487555
proteins of the sieve-tube exudate of cucurbita maxima.	sieve-tube exudate which appears on cut surfaces of stems of cucurbita maxima as distinct droplets has been depicted in electron micrographs of longitudinal sections of the phloem. the exudate, which was produced from mature sieve tubes only, contained filaments of p-protein, but no mitochondria or vesicles of endoplasmic reticulum. the water-soluble part of the exudate contained at least 12 proteins, as shown by disc-electrophoresis. enzymic activity was found for peroxidases, acid phosphatases ...	1971	24488194
protein filaments-structural components of the phloem exudate : i. observations with cucurbita and nicotiana.	fine structure and chemical composition of the phloem exudate of cucurbita maxima and nicotaiana glauca x suaveolens are investigated. filamentous structures, several microns in length, are identified as structural components of the exudate by means of negative staining and electron microscopy. two types of filaments are described: one form measures nearly 40 å in diameter and shows a beaded appearance with regular spacings of about 50 å; it is termed "elementary filament". the second form has a ...	1970	24497023
[spectrophotometric analysis of phytochrome in the embryo of cucurbita pepo and cucurbita maxima].	phytochrome detection and △ (△o.d.) measurements were made for the first time in a single embryo of pumpkin or gourd thanks to an apparatus adjusted to the differential spectrophotometer constructed by dr. spruit (wageningen). observed △ (△o.d.) values are comparable with usual values obtained by analysis of several seeds in a cuvette. the differential spectrum obtained has the same characteristics as the spectra of seedlings and seeds which can be found in the present literature. the time cours ...	1970	24499981
three new triterpene esters from pumpkin (cucurbita maxima) seeds.	three new multiflorane-type triterpene esters, i.e. 7α-hydroxymultiflor-8-ene-3α,29-diol 3-acetate-29-benzoate (1), 7α-methoxymultiflor-8-ene-3α,29-diol 3,29-dibenzoate (2), and 7β-methoxymultiflor-8-ene-3α,29-diol 3,29-dibenzoate (3), were isolated from seeds of cucurbita maxima, along with the known compound, multiflora-7,9(11)-diene-3α,29-diol 3,29-dibenzoate (4). compound 1 exhibited melanogenesis inhibitory activities comparable with those of arbutin. in cytotoxicity assays, compounds 1 and ...	2014	24743937
pumpkin seed oil extracted from cucurbita maxima improves urinary disorder in human overactive bladder.	the pumpkin seed oil obtained from cucurbita pepo has been shown to be useful for the treatment of nocturia in patients with urinal disorders in several western countries. in this study, we evaluated the effect of the pumpkin seed oil from cucurbita maxima on urinary dysfunction in human overactive bladder (oab). forty-five subjects were enrolled in this study. an extract of pumpkin seed oil from c. maxima (10 g of oil/day) was orally administrated for 12 weeks. after 6 and 12 weeks, urinary fun ...	2014	24872936
mutational analysis of the pumpkin (cucurbita maxima) phloem exudate lectin, pp2 reveals ser-104 is crucial for carbohydrate binding.	the pumpkin phloem lectin (pp2) is an rna-binding, defense-related, chitooligosaccharide-specific, homodimeric lectin of mr 48 kda expressed at high concentrations in the sieve elements and companion cells of pumpkin (cucurbita maxima). in the present study, pp2 was expressed in the methylotrophic yeast pichia pastoris with the saccharomyces α-factor sequence to direct the recombinant protein into the secretory pathway as a prerequisite for unimpaired folding and posttranslational glycosylation ...	2014	24950405
description of ektaphelenchoides fuchsi n. sp. (nematoda: ektaphelenchinae) from western iran.	ektaphelenchoides fuchsi n. sp., recovered from a soil sample around the rhizosphere of cucurbita maxima in western iran, is described and illustrated based on morphological and molecular characters. the new species is characterized by its body length of 529-712 µm, continuous lip region, lateral fields with three incisures, total stylet length of 14-18 µm with rounded basal knobs, excretory pore 70-84 µm and hemizonid 87-96 µm from the anterior end, post-uterine sac short, 6-9 µm long in female ...	2014	25112260
structural divergence of plant tctps.	the translationally controlled tumor protein (tctp) is a highly conserved protein at the level of sequence, considered to play an essential role in the regulation of growth and development in eukaryotes. however, this function has been inferred from studies in a few model systems, such as mice and mammalian cell lines, drosophila and arabidopsis. thus, the knowledge regarding this protein is far from complete. in the present study bioinformatic analysis showed the presence of one or more tctp ge ...	2014	25120549
regulatory control of carotenoid accumulation in winter squash during storage.	storage promotes carotenoid accumulation and converts amylochromoplasts into chromoplasts in winter squash. such carotenoid enhancement is likely due to continuous biosynthesis along with reduced turnover and/or enhanced sequestration. postharvest storage of fruits and vegetables is often required and frequently results in nutritional quality change. in this study, we investigated carotenoid storage plastids, carotenoid content, and its regulation during 3-month storage of winter squash butternu ...	2014	25139277
changes in carotenoids during processing and storage of pumpkin puree.	changes in the contents of carotenoids and their true retentions (% tr) during the production of puree of cucurbita moschata 'menina brasileira' and of cucurbita maxima 'exposição' pumpkins and the stability of such compounds during 180days of storage were monitored by liquid chromatography coupled with a photodiode array detector. cooking caused higher losses than commercial sterilisation. high losses of xanthophylls such as lutein and violaxanthin were noted during processing and storage of pu ...	2011	25214348
systemic delivery of sirna in pumpkin by a plant phloem small rna-binding protein 1-ribonucleoprotein complex.	in plants, the vascular system, specifically the phloem, functions in delivery of small rna (srna) to exert epigenetic control over developmental and defense-related processes. although the importance of systemic srna delivery has been established, information is currently lacking concerning the nature of the protein machinery involved in this process. here, we show that a phloem small-rna binding protein 1 (psrp1) serves as the basis for formation of an srna ribonucleoprotein complex (srnpc) th ...	2014	25227635
cucumber (cucumis sativus l.) and kabocha squash (cucurbita moschata duch).	we established improved methods for agrobacterium-mediated transformation of cucumber (cucumis sativus l.) and kabocha squash (cucurbita moschata duch). vacuum infiltration of cotyledonary explants with agrobacterium suspension enhanced the agrobacterium infection efficiency in the proximal regions of explants. wounding treatment was also essential for kabocha squash. cocultivation on filter paper wicks suppressed necrosis of explants, keeping regeneration efficacy. putative transgenic plants we ...	2015	25300850
11s storage globulin from pumpkin seeds: regularities of proteolysis by papain.	limited proteolysis of the α- and β-chains and deep cleavage of the αβ-subunits by the cooperative (one-by-one) mechanism was observed in the course of papain hydrolysis of cucurbitin, an 11s storage globulin from seeds of the pumpkin cucurbita maxima. an independent analysis of the kinetics of the limited and cooperative proteolyses revealed that the reaction occurs in two successive steps. in the first step, limited proteolysis consisting of detachments of short terminal peptides from the α- a ...	2014	25365492
overexpression of the pumpkin (cucurbita maxima) 16 kda phloem protein cmpp16 increases tolerance to water deficit.	the phloem plays an important role in the delivery of nutrients and signals between photosynthetic to heterotrophic tissues. proteins and rnas in the phloem translocation stream may have an important role in maintaining the integrity of the sieve tube system, as well as in long-distance signaling. cmpp16 is a pumpkin phloem protein, which has been shown to bind rna in a non-sequence specific manner, and move it cell-to-cell and conceivably, long-distance. the protein and rna are found in both co ...	2014	25482781
instrumental and sensory analyses of quality attributes of grafted specialty melons.	soilborne disease management remains a great challenge in melon production with the phaseout of soil fumigant methyl bromide. grafting has been shown to be an effective approach to control soilborne diseases. however, previous research has yielded mixed results regarding the impacts of rootstock on fruit quality. very few studies have assessed melon quality attributes using both sensory evaluation and instrumental methods.	2015	25512001
aphid salivary proteases are capable of degrading sieve-tube proteins.	sieve tubes serve as transport conduits for photo-assimilates and other resources in angiosperms and are profitable targets for piercing-sucking insects such as aphids. sieve-tube sap also contains significant amounts of proteins with diverse functions, for example in signalling, metabolism, and defence. the identification of salivary proteases in acyrthosiphon pisum led to the hypothesis that aphids might be able to digest these proteins and by doing so suppress plant defence and access additio ...	2015	25540441
the making of giant pumpkins: how selective breeding changed the phloem of cucurbita maxima from source to sink.	despite the success of breeding programmes focused on increasing fruit size, relatively little is known about the anatomical and physiological changes required to increase reproductive allocation. to address this gap in knowledge, we compared fruit/ovary anatomy, vascular structure and phloem transport of two varieties of giant pumpkins, and their smaller fruited progenitor under controlled environmental conditions. we also modelled carbon transport into the fruit of competitively grown plants u ...	2015	25546629
effects of grafting with pumpkin rootstock on carbohydrate metabolism in cucumber seedlings under ca(no3)2 stress.	this study investigated the effects of grafting on the carbohydrate status and the enzymes of carbohydrate metabolism in self-grafted and grafted cucumber seedlings using the salt-tolerant pumpkin rootstock 'qingzhen 1' (cucurbita maxima × cucurbita moschata) under 80 mm ca(no3)2 stress for 6 d. the growth of self-grafted seedlings was significantly inhibited after the treatment of ca(no3)2 stress, whereas the inhibition of growth was alleviated in pumpkin rootstock-grafted seedlings. ca(no3)2 s ...	2015	25579659
evaluation of cucurbita maxima extract against scopolamine-induced amnesia in rats: implication of tumour necrosis factor alpha.	cucurbita maxima (cm) seed oil is commonly used in indian folk medicine to treat various ailments. we have investigated the effect of cm seed oil on memory impairment induced by scopolamine in rats. male adult wistar rats were administered scopolamine 1 mg/kg body weight, i.p. or 1.25 mg/kg body weight, s.c. to induce memory impairment. the nootropic agent piracetam 100 mg/kg body weight, i.p. and cm seed oil 100 and 200 mg/kg body weight, p.o. were administered daily for five consecutive days. ...	2015	25711042
high throughput sequencing of small rnas in the two cucurbita germplasm with different sodium accumulation patterns identifies novel micrornas involved in salt stress response.	micrornas (mirnas), a class of small non-coding rnas, recognize their mrna targets based on perfect sequence complementarity. mirnas lead to broader changes in gene expression after plants are exposed to stress. high-throughput sequencing is an effective method to identify and profile small rna populations in non-model plants under salt stresses, significantly improving our knowledge regarding mirna functions in salt tolerance. cucurbits are sensitive to soil salinity, and the cucurbita genus is ...	2015	26010449
a review on omega-3 and omega-6 essential fatty acids: uses, benefits and their availability in pumpkins (cucurbita maxima) seed and desert dates (balanites aegyptiaca) seed kernel oils.		2014	26027166
physico-chemical properties and fatty acid composition of pomegranate, cherry and pumpkin seed oils.	nut and seed oils are often considered waste products but in recent years they have been receiving growing interest due to their high concentration of hydrophilic and lipophilic bioactive components, which have important pharmacological properties on human health. the aim of this work was to compare the physico-chemical and biochemical properties of pomegranate (punicagranatum), sweet cherry (prunusavium) and pumpkin (cucurbita maxima) seed oils obtained by solvent extraction.	2016	26033409
hplc-dad-ms(n) characterisation of carotenoids from apricots and pumpkins for the evaluation of fruit product authenticity.	carotenoids including carotenoid esters from six apricot (prunus armeniaca l.) cultivars and from eight cultivars from three pumpkin species (cucurbita maxima duch., cucurbita pepo l., and cucurbita moschata duch.) were extracted without saponification, separated on a c-30 reversed-phase column and characterised by high-performance liquid chromatography/atmospheric pressure chemical ionisation-mass spectrometry (lc-ms). the predominant free carotenoids were quantified by hplc with diode array de ...	2008	26049248
short communication: genetic linkage map of cucurbita maxima with molecular and morphological markers.	cucurbita maxima is one of the most widely cultivated vegetables in china and exhibits distinct morphological characteristics. in this study, genetic linkage analysis with 57 simple-sequence repeats, 21 amplified fragment length polymorphisms, 3 random-amplified polymorphic dna, and one morphological marker revealed 20 genetic linkage groups of c. maxima covering a genetic distance of 991.5 cm with an average of 12.1 cm between adjacent markers. genetic linkage analysis identified the simple-seq ...	2015	26125744
effect of african leafy vegetables on the micronutrient status of mildly deficient farm-school children in south africa: a randomized controlled study.	a randomized controlled trial was conducted to assess the effect of african leafy vegetable (alv) consumption on fe, zn and vitamin a status in children.	2016	26146039
biologically synthesised silver nanoparticles from three diverse family of plant extracts and their anticancer activity against epidermoid a431 carcinoma.	biological synthesis of silver nanoparticles is a cost effective natural process where the phytochemicals specifically phenols, flavonoids and terpenoids present in the plant extracts act as capping and reducing agent. due to their nano size regime the silver nanoparticles may directly bind to the dna of the pathogenic bacterial strains leading to higher antimicrobial activity.	2015	26196716