Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| changes in the cotyledons of cucurbita maxima during germination. v. the nuclear envelope. | 1975 | 1152111 | |
| purification of ent-kaurene oxidase from gibberella fujikuroi and cucurbita maxima. | 1992 | 1397591 | |
| ascorbic acid biosensor using ascorbate oxidase immobilized on alkylamine glass beads. | a biosensor for ascorbic acid based on enzyme kinetics of ascorbate oxidase (e.c.1.10.3.3) was developed. the enzyme was extracted from cucurbita maxima, or jerimun and immobilized by covalent bounding, using glutaradehyde as a bifunctional agent, on alkylamine glass beads, with and without enzyme active site protection. a low-cost, home-made oxygen electrode was applied as a transducer. the system has sensitivity from 62.5 up to 500 microm of ascorbic acid with satisfactory operation for more t ... | 2013 | 1416950 |
| new analogues of cucurbita maxima trypsin inhibitor iii (cmti iii) with simplified structure. | seven new analogues of trypsin inhibitor cmti iii were obtained by solid-phase peptide synthesis. three analogues contained only two, instead of three, disulfide bridges, whereas the molecules of the next four analogues were shortened at the n- and/or c-terminus. the elimination of one disulfide bridge in cmti iii induces a decrease in the association equilibrium constants by 6-7 orders of magnitude, whereas the removal of one, two or three amino-acid residues at the n- and/or c-terminus does no ... | 2006 | 1418676 |
| homonuclear three-dimensional noe-noe nuclear magnetic resonance/spectra for structure determination of proteins in solution. | the solution structures of two proteins (cmti-i, a trypsin inhibitor from cucurbita maxima, and hisactophilin, an actin binding protein of 118 amino acids) have been determined based on the noe data derived solely from the homonuclear 3d noe-noe magnetic resonance spectroscopy. two different approaches for extraction of the structural information from the 3d noe-noe experiment were tested. one approach was based on the transformation of the 3d intensities into distance constraints. in the second ... | 1992 | 1447779 |
| structural consequences of the natural substitution, e9k, on reactive-site-hydrolyzed squash (cucurbita maxima) trypsin inhibitor (cmti), as studied by two-dimensional nmr. | sequence-specific hydrogen-1 nmr assignments were made to all of the 29 amino acid residues of reactive-site-hydrolyzed cucurbita maxima trypsin inhibitor i (cmti-i*) by the application of two-dimensional nmr (2d nmr) techniques, and its secondary structural elements (two tight turns, a 3(10)-helix, and a triple-stranded beta-sheet) were identified on the basis of short-range noesy cross peaks and deuterium-exchange kinetics. these secondary structural elements are present in the intact inhibito ... | 1992 | 1599921 |
| natural abundance 15n nmr assignments delineate structural differences between intact and reactive-site hydrolyzed cucurbita maxima trypsin inhibitor iii. | 15n nmr assignments were made to the backbone amide nitrogen atoms at natural isotopic abundance of intact and reactive-site (arg5-ile6) hydrolyzed cucurbita maxima trypsin inhibitor iii (cmti-iii and cmti-iii*, respectively) by means of 2d proton-detected heteronuclear single bond chemical shift correlation (hsbc) spectroscopy, utilizing the previously made sequence-specific 1h nmr assignments (krishnamoorthi et al. (1992) biochemistry 31, 898-904). comparison of the 15n chemical shifts of the ... | 1992 | 1618315 |
| two-dimensional nmr studies of squash family inhibitors. sequence-specific proton assignments and secondary structure of reactive-site hydrolyzed cucurbita maxima trypsin inhibitor iii. | the solution structure of reactive-site hydrolyzed cucurbita maxima trypsin inhibitor iii (cmti-iii*) was investigated by two-dimensional proton nuclear magnetic resonance (2d nmr) spectroscopy. cmti-iii*, prepared by reacting cmti-iii with trypsin which cleaved the arg5-ile6 peptide bond, had the two fragments held together by a disulfide linkage. sequence-specific 1h nmr resonance assignments were made for all the 29 amino acid residues of the protein. the secondary structure of cmti-iii*, as ... | 1992 | 1731946 |
| proton nmr studies of cucurbita maxima trypsin inhibitors: evidence for ph-dependent conformational change and his25-tyr27 interaction. | a ph-dependent his25-tyr27 interaction was demonstrated in the case of cucurbita maxima trypsin inhibitors (cmti-i and cmti-iii) by means of nuclear magnetic resonance (nmr) spectroscopy. ph titration, line widths, peak shapes, deuterium exchange kinetics, and two-dimensional nuclear overhauser effect spectroscopy (noesy) were employed to characterize a conformational change involving tyr27, which was shown to be triggered by deprotonation of his25 around ph 6. a hydrogen bond is proposed to be ... | 1992 | 1731947 |
| molecular cloning of an 1-aminocyclopropane-1-carboxylate synthase from senescing carnation flower petals. | synthetic oligonucleotides based on the sequence of 1-aminocyclopropane-1-carboxylate (acc) synthase from tomato were used to prime the synthesis and amplification of a 337 bp tomato acc synthase cdna by polymerase chain reaction (pcr). this pcr product was used to screen a cdna library prepared from mrna isolated from senescing carnation flower petals. two cdna clones were isolated which represented the same mrna. the longer of the two clones (caracc3) contained a 1950 bp insert with a single o ... | 1992 | 1731995 |
| screening of the antimalarial activity of plants of the cucurbitaceae family. | crude ethanolic extracts (cees) from two species of cucurbitaceae, cucurbita maxima and momordica charantia (commonly called "abóbora moranga" and "melão de são caetano", respectively) were assayed for antimalarial activity by the 4-d suppressive test. the cee of dry c. maxima seeds showed strong antimalarial activity following oral administration (250 and 500 mg/kg), reducing by 50% the levels of parasitemia in plasmodium berghei-infected mice. treatment of normal animals with 500 mg/kg of the ... | 1991 | 1841996 |
| synthesis of a wild-type and three mutant cucurbita maxima trypsin inhibitor-encoding genes by a single-strand approach. | a single-strand approach to gene assembly, based on a modification of an in vitro complementary oligodeoxyribonucleotide template-directed ligation of the desired sequence to a linearized vector [chen et al., nucleic acids res. 18 (1990) 871-878], is described. the gene coding for the wild-type cucurbita maxima trypsin inhibitor of 29 amino acid residues [bode et al., febs lett. 242 (1989) 285-292], as well as three mutant forms of the gene, in which two of the three disulfide bonds have been re ... | 1991 | 1937021 |
| heterogeneity and organization of the ribosomal rna genes of cucurbita maxima. | thirty-six clones were recovered from cucurbita maxima genomic dna which had been enriched for rdna and cleaved at the unique repeat unit hind iii site. twenty-nine of these, which contain complete rdna units, were compared to a standard whose intergenic spacer (igs) nucleotide sequence has been determined. twenty-one are identical in length and restriction site pattern. eight which differ from the standard in length do so because of addition or deletion of varying numbers of igs subrepetitive u ... | 1990 | 2102876 |
| monitoring protein cleavage and concurrent disulfide bond assignment using thermospray lc/ms. | the arrangement of the disulfide bridges of cucurbita maxima trypsin inhibitor, cmti i, has been confirmed by enzymatic and chemical cleavages of the native protein and analysis of the resulting disulfide-bridged fragments using thermospray liquid chromatography/mass spectrometry. although the disulfide bridges of cmti i have recently been assigned from the x-ray crystallographic structure, direct chemical analysis of the s-s bonds using classical techniques proved difficult. the cmti i molecule ... | 2007 | 2134058 |
| a new protein inhibitor of trypsin and activated hageman factor from pumpkin (cucurbita maxima) seeds. | a protein inhibitor (cmti-v; mr 7106) of trypsin and activated hageman factor (factor xiia), a serine protease involved in blood coagulation, has been isolated for the first time from pumpkin (cucurbita maxima) seeds by means of trypsin-affinity chromatography and reverse phase high performance liquid chromatography (hplc). the dissociation constants of the inhibitor complexes with trypsin and factor xiia have been determined to be 1.6 x 10(-8) and 4.1 x 10(-8) m, respectively. the primary struc ... | 1990 | 2226848 |
| inhibition of human beta-factor xiia by squash family serine proteinase inhibitors. | many inhibitors of trypsin and human beta-factor xiia have been isolated from squash and related seeds and sequenced (wieczorek et al., biochem. biophys. res. comm. (1985) 126, 646-652). the association equilibrium constants (ka) of several of these inhibitors have now been determined with human beta-factor xiia using a modification of the method of green and work (park et al., fed. proc. fed. am. soc. exp. biol. (1984) 43, 1962). the ka's range from 7.8 x 10(4) m-1 to 3.3 x 10(8) m-1. two isoin ... | 1990 | 2306254 |
| isolation and serological comparison of virus-coded proteins of three potyviruses infecting cucurbitaceous plants. | cylindrical inclusion proteins (cips), amorphous inclusion proteins (aips), and virus particles were partially purified from cucurbita maxima leaves infected with zucchini yellow mosaic virus (zymv), watermelon mosaic virus 2 (wmv2), and the watermelon mosaic virus 1 strain of papaya ringspot virus (prsv-w). antisera to these individual antigens, except aips of zymv and wmv2, were prepared and used to determine with electroblot-elisa the serological differentiation indices (sdis) between homolog ... | 1990 | 2338401 |
| the cucurbita maxima ribosomal dna intergenic spacer has a complex structure. | the nucleotide (nt) sequence of the 5508-nt intergenic spacer (igs), between the 25s- and the 18s-coding regions of cucurbita maxima rdna, was determined. the fragment sequenced is 6142 nt long and includes 472 nt of 25s- and 162 nt of 18s-coding regions. the igs has a complex primary structure, composed of five repetitive families (a-e) and three unique domains. it is dominated by the presence of nine, tandemly-repeating units of approximately 250 nt (repeat d), each unit containing four copies ... | 1989 | 2583512 |
| 1h-n.m.r. studies of squash seed trypsin inhibitor. | 1h-n.m.r. studies at 500 mhz have been performed on a trypsin inhibitor (cmti-iii) found in squash seed (cucurbita maxima). the sequential resonance assignments have been made using two-dimensional techniques. the chemical shifts for the assigned protons are reported at 30 degrees, ph 2.8 and form a basis for the determination of the solution structure of cmti-iii. analysis of the noe data, nh-alpha ch vicinal coupling constants and pattern of slowly exchanging amide protons indicates that the p ... | 2008 | 2613440 |
| determination of the complete three-dimensional structure of the trypsin inhibitor from squash seeds in aqueous solution by nuclear magnetic resonance and a combination of distance geometry and dynamical simulated annealing. | the complete three-dimensional structure of the trypsin inhibitor from seeds of the squash cucurbita maxima in aqueous solution was determined on the basis of 324 interproton distance constraints, 80 non-nuclear overhauser effect distances, and 22 hydrogen-bonding constraints, supplemented by 27 phi backbone angle constraints derived from nuclear magnetic resonance measurements. the nuclear magnetic resonance input data were converted to the distance constraints in a semiquantitative manner afte ... | 1989 | 2614837 |
| nuclear magnetic resonance solution and x-ray structures of squash trypsin inhibitor exhibit the same conformation of the proteinase binding loop. | a comparison of the solution nuclear magnetic resonance (n.m.r.) structures of squash trypsin inhibitor from seeds of the squash cucurbita maxima with the x-ray structure of a trypsin complex of the inhibitor shows that the n.m.r. and x-ray structures are similar in terms of the global folding and secondary structure. the average atomic root-mean-square difference between the 36 n.m.r. structures on the one hand and the x-ray structure is 0.96 a for the backbone atoms and 1.95 a for all heavy at ... | 1989 | 2614838 |
| [characterization of xanthomonas campestris pv. cucurbitae, causal agent of the bacterial spot of squash]. | bacterial leaf spot of squash was characterized for the first time in argentina. cultural, physiological, morphological and cross-infection tests on cucurbitaceae showed that the pathogen was xanthomonas campestris pv. cucurbitae (bryan) dye. the bacterium isolated from winter squash proved pathogenic for pumpkin, winter squash, cucumber and watermelon but no for muskmelon. | 1989 | 2748850 |
| the refined 2.0 a x-ray crystal structure of the complex formed between bovine beta-trypsin and cmti-i, a trypsin inhibitor from squash seeds (cucurbita maxima). topological similarity of the squash seed inhibitors with the carboxypeptidase a inhibitor from potatoes. | the stoichiometric complex formed between bovine beta-trypsin and the cucurbita maxima trypsin inhibitor i (cmti-i) was crystallized and its x-ray crystal structure determined using patterson search techniques. its structure has been crystallographically refined to a final r value of 0.152 (6.0-2.0 a). cmti-i is of ellipsoidal shape; it lacks helices or beta-sheets, but consists of turns and connecting short polypeptide stretches. the disulfide pairing is cys-3i-20i, cys-10i-22i and cys-16i-28i. ... | 1989 | 2914611 |
| identification of a dispersed mboi repeat family in five higher plant genomes. | digestion of nuclear dnas of five plants, namely cucurbita maxima (red gourd), trichosanthes anguina (snake gourd), cucumis sativus (cucumber), cajanus cajan (pigeon pea) and phaseolus vulgaris (french bean) with the restriction endonuclease mboi yielded discrete size classes with molecular weights in the range of 0.5 to 5 kbp. the mboi digestion pattern of cot 0.1 dna in french bean is comparable with that of total dna, indicating that these bands represented highly repeated dna sequences. clea ... | 1988 | 3233342 |
| [obtention and evaluation of a precooked flour of auyama (cucurbita maxima) and rice, enriched with oleaginous proteins and/or skim milk]. | the purpose of the present study was the production of a precooked blend made of pumpkin squash and rice flour (prb), processed with drum dyer. the blend was supplemented with different protein sources: soy, sesame and deffated milk. the following formulations were obtained: i) prb + 10.5% deffated soy flour; ii) prb + 15% deffated milk; iii) prb + 5% deffated sesame flour + 10% deffated milk; iv) prb + 5% deffated soy flour + 10% deffated sesame flour, and v) prb + 9.5% deffated soy flour + 9.5 ... | 1988 | 3256284 |
| two linear plasmids in mitochondria of fusarium solani f. sp. cucurbitae. | two linear plasmid-like dnas designated pfsc1 (9.2 kbp) and pfsc2 (8.3 kbp) were found in an isolate of the plant pathogenic fungus fusarium solani f. sp. cucurbitae race 1. the plasmids were maternally inherited and copurified with mitochondrial dna obtained from a mitochondria-enriched cell fraction suggesting that they are located in mitochondria. the plasmids did not share extensive sequence similarity. no homology was detected between either plasmid and the nuclear or mitochondrial genome w ... | 1988 | 3399553 |
| effects of 60-hz electric fields on cellular elongation and radial expansion growth in cucurbit roots. | serial longitudinal and transverse sections were prepared from roots of cucumis sativus and cucurbita maxima that had been exposed/sham-exposed to 60-hz electric fields for 0-2 days. field exposures were selected to produce a 10-20% or a 70-80% growth inhibition in whole roots of both species. cortical cell length and diameter were measured using a microscope and eyepiece micrometer; measurements were conducted "blind." in both species, inhibition of cellular elongation was associated with expos ... | 1987 | 3580000 |
| cucurbitacin delta 23-reductase from the fruit of cucurbita maxima var. green hubbard. physicochemical and fluorescence properties and enzyme-ligand interactions. | cucurbitacin delta 23-reductase from cucurbita maxima var. green hubbard fruit displays an apparent mr of 32,000, a stokes radius of 263 nm and a diffusion coefficient of 8.93 x 10(-7) cm2 x s-1. the enzyme appears to possess a homogeneous dimeric quaternary structure with a subunit mr of 15,000. two tryptophan and fourteen tyrosine residues per dimer were found. emission spectral properties of the enzyme and fluorescence quenching by iodide indicate the tryptophan residues to be buried within t ... | 1986 | 3707515 |
| an improved enzymatic synthesis of labeled gibberellin a12-aldehyde and gibberellin a12. | the biochemical synthesis of labeled gibberellin a12-7-aldehyde (ga12ald) and and gibberellin a12 (ga12) from labeled r,s-mevalonic acid (mva) using cell-free extracts from pumpkin (cucurbita maxima) endosperm has been improved over the previously reported procedure. three major improvements were developing a one-step hplc procedure to isolate ga12ald and ga12 in radiochemically pure form; adjusting the ph of the reaction mix to ph 6.9 which increased the ga12ald/ga12 ratio over that at ph 7.8 b ... | 1986 | 3754395 |
| induction of elf transmembrane potentials in relation to power-frequency electric field bioeffects in a plant root model system. i. relationship between applied field strength and cucurbitaceous root growth rates. | seminal roots of cucumis sativus and cucurbita maxima were exposed to 60 hz electric fields of 100-500 v x m-1 in a conducting aqueous inorganic growth medium. root growth rates were measured to produce a dose-response relationship for each species. the species were selected for study because of their familial relationship, reported sensitivity to 60 hz, 360 v x m-1 electric fields, and differing average root cell sizes. the latter characteristic influences the magnitude of elf membrane potentia ... | 1986 | 3763827 |
| induction of elf transmembrane potentials in relation to power-frequency electric field bioeffects in a plant root model system. ii. the effect of 60 hz electric fields on the growth of different regions of the cucurbit root elongation zone. | the region of elongation in cucumis sativus and cucurbita maxima roots was marked at increasing distances from the apex to provide an analog of increasing cell size. these roots were exposed/sham-exposed to 60 hz electric fields and the growth rates of the root segments measured. the growth rate effect magnitude varied with increasing distance from the root tip at constant field strength, and with increasing applied field strength. these results provide strong, qualitative support for the postul ... | 1986 | 3763828 |
| quaternary structure and composition of squash nadh:nitrate reductase. | nadh:nitrate reductase (ec 1.6.6.1) was isolated from squash cotyledons (cucurbita maxima l.) by a combination of blue sepharose and zinc-chelate affinity chromatographies followed by gel filtration on bio-gel a-1.5m. these preparations gave a single protein staining band (mr = 115,000) on sodium dodecyl sulfate gel electrophoresis, indicating that the enzyme is homogeneous. the native mr of nitrate reductase was found to be 230,000, with a minor form of mr = 420,000 also occurring. these result ... | 1985 | 4038708 |
| screening of trichosanthes kirilowii, momordica charantia and cucurbita maxima (family cucurbitaceae) for compounds with antilipolytic activity. | saline extracts of trichosanthes kirilowii root tubers and momordica charantia seeds were subjected to differential acetone fractionation. the fraction that precipitated after addition of 0.8 vol. acetone was designated api, the fraction that precipitated after addition of another 1.2 vol. acetone was designated apii, and the fraction that precipitated after addition of another volume acetone was designated apiii. api, apii and apiii are fractions enriched with lectins, abortifacient proteins an ... | 1985 | 4058034 |
| structure and biochemistry of phloem-proteins isolated from cucurbita maxima. | 1974 | 4210435 | |
| [on the soluble proteins in the water from seeds of cucurbita maxima duch]. | 1966 | 4225958 | |
| cucurbitacin b delta 23-reductase from cucurbita maxima. ii. cofactor requirements, enzyme kinetics, substrate specificity and other characteristics. | 1968 | 4384331 | |
| the amino acid sequence of cytochrome c from cucurbita maxima l. (pumpkin). | the amino acid sequence of pumpkin cytochrome c was determined on 2mumol of protein. some evidence was found for the occurrence of two forms of cytochrome c, whose sequences differed in three positions. pumpkin cytochrome c consists of 111 residues and is homologous with mitochondrial cytochromes c from other plants. experimental details are given in a supplementary paper that has been deposited as supplementary publication sup 50005 at the national lending library for science and technology, bo ... | 1971 | 5131733 |
| artificial tetraploids of squash (cucurbita maxima duch.). | 1971 | 5163382 | |
| [soluble proteins of the seed of gourd (cucurbita maxima duch.)]. | 1970 | 5429059 | |
| [insoluble proteins of gourd seeds (cucurbita maxima duch)]. | 1970 | 5429060 | |
| cucurbitacin b delta 23-reductase from cucurbita maxima. i. assay methods, isolation and purification. | 1968 | 5640163 | |
| fractionation of fatty acids of cucurbita maxima seed oil with urea. | 1968 | 5641684 | |
| p protein in the phloem of cucurbita. ii. the p protein of mature sieve elements. | during maturation of sieve elements in cucurbita maxima duchesne, the p-protein bodies (slime bodies) usually disperse in the tonoplast-free cell. in some sieve elements the p-protein bodies fail to disperse. the occurrence of dispersal or nondispersal of p-protein bodies can be related to the position of the sieve elements in the stem or petiole. in the sieve elements within the vascular bundle the bodies normally disperse; in the extrafascicular sieve elements the bodies often fail to disperse ... | 1968 | 5664205 |
| p protein in the phloem of cucurbita. i. the development of p-protein bodies. | light and electron microscopical observations of the cells of the phloem of cucurbita maxima have shown that two distinct types of p-protein bodies are formed: a larger type which arises as fine fibrils and a smaller type which apparently arises as groups of tubules. the tubules of the smaller type of body measure 242 +/- 3.6 (se) a (n = 48) and appear morphologically identical with the p1-protein tubules of nicotiana tabacum l. in some of these p1-protein bodies the tubules are arranged in a re ... | 1968 | 5675415 |
| anthelmintic activity of cucurbita maxima (kaddu) seeds. | 1967 | 6076370 | |
| effect of pumpkin mosaic virus infection on the hill reaction and primary productivity of pumpkin (cucurbita maxima duch.) leaves. | 1982 | 6184958 | |
| pumpkin seed inhibitor of human factor xiia (activated hageman factor) and bovine trypsin. | a strong inhibitor of human hageman factor fragment (hff, beta-factor xiia) and bovine trypsin was isolated from pumpkin (cucurbita maxima) seed extracts by acetone fractionation, by chromatography on columns of diethyl-aminoethylcellulose and carboxylmethyl-sephadex c-25, and by sephadex g-50 gel filtration. pumpkin seed hageman factor inhibitor (phfi) is unusual in its lack of inhibition of several other serine proteinases tested--human plasma, human urinary, and porcine pancreatic kallikreins ... | 1982 | 6215935 |
| isolation and partial amino acid sequence of the trypsin inhibitor from the seeds of cucurbita maxima. | trypsin inhibitor from squash (cucurbita maxima) seed was extracted with 0.1 m-acetate buffer, ph 4.5, purified on immobilized trypsin, and separated by se-sephadex c-50 chromatography into three active fractions. all of them inhibited trypsin to the same extent, showed no antichymotrypsin or antikallikrein activity, had a similar molecular weight (about 3300), and contained no tryptophan, phenylalanine or threonine. the partial amino acid sequence of tryptic and peptic peptides of fraction iii ... | 1980 | 6791422 |
| amino-acid sequence of two trypsin isoinhibitors, itd i and itd iii from squash seeds (cucurbita maxima). | the amino-acid sequences of two trypsin isoinhibitors, itd i and itd iii, from squash seeds (cucurbita maxima) were determined. both isoinhibitors contain 29 amino-acid residues, including 6 half cystine residues. they differ only by one amino acid. lysine in position 9 of itd iii is substituted by glutamic acid in itd i. arginine in position 5 is present at the reactive site of both isoinhibitors. the previously published sequence of itd iii has been shown to be incorrect. | 1983 | 6840699 |
| preparation and characteristics of trypsin inhibitors from the seeds of squash (cucurbita maxima) and zucchini (cucurbita pepo var. giromontia). | one trypsin inhibitor (i) from zucchini seeds and two (i and iii) from squash seeds were isolated by ammonium sulphate salting out and chromatography on sp-sephadex c-25 and immobilized trypsin. the inhibitors have the same molecular mass, about 3300, contain 29 amino acids, have three disulphide bridges, and lack thr, phe and trp. the isoelectric point of the zucchini inhibitor i and squash inhibitor i is of 5.6, whereas that of the squash inhibitor iii--of 8.3. arg is the n-terminal amino acid ... | 1983 | 6868907 |
| subunit structure and interactions of the phloem proteins of cucurbita maxima (pumpkin). | the two major proteins from the phloem exudate of cucurbita maxima (pumpkin), pp1 and pp2, were stable in the absence of reducing agents after modification of their accessible cysteine residues with iodoacetamide. this permitted their purification without precautions to prevent oxidation. pp2, a lectin specific for oligomers of n-acetyl-d-glucosamine, was shown by sedimentation-equilibrium ultracentrifugation to be a dimer of mr of 48000. neither dithiothreitol nor tri-(n-acetyl-d-glucosamine) a ... | 1983 | 6884347 |
| trypsin inhibitor iii from squash seeds (cucurbita maxima), its reactive site and proposed amino acid sequence. | the amino acid sequence of trypsin inhibitor iii isolated from squash seeds (cucurbita maxima) was established by edman degradation and standard enzymatic and chemical techniques. the inhibitor consists of 28 amino acids crosslinked by two disulfide bridges. arginine in position 5 was shown to be present at the reactive site of the inhibitor. | 1981 | 7275008 |
| reactive-site hydrolyzed cucurbita maxima trypsin inhibitor-v: function, thermodynamic stability, and nmr solution structure. | reactive-site (lys44-asp45 peptide bond) hydrolyzed cucurbita maxima trypsin inhibitor-v (cmti-v*) was prepared and characterized: in comparison to the intact form, cmti-v* exhibited markedly reduced inhibitory properties and binding affinities toward trypsin and human blood coagulation factor xiia. the equilibrium constant of trypsin-catalyzed hydrolysis, khyd, defined as [cmti-v*]/[cmti-v], was measured to be approximately 9.4 at 25 degrees c (delta g degrees = -1.3 kcal.mol-1). from the tempe ... | 1995 | 7547948 |
| the ga5 locus of arabidopsis thaliana encodes a multifunctional gibberellin 20-oxidase: molecular cloning and functional expression. | the biosynthesis of gibberellins (gas) after ga12-aldehyde involves a series of oxidative steps that lead to the formation of bioactive gas. previously, a cdna clone encoding a ga 20-oxidase [gibberellin, 2-oxoglutarate:oxygen oxidoreductase (20-hydroxylating, oxidizing), ec 1.14.11.-] was isolated by immunoscreening a cdna library from liquid endosperm of pumpkin (cucurbita maxima l.) with antibodies against partially purified ga 20-oxidase. here, we report isolation of a genomic clone for ga 2 ... | 1995 | 7604047 |
| isolation and expression of three gibberellin 20-oxidase cdna clones from arabidopsis. | using degenerate oligonucleotide primers based on a pumpkin (cucurbita maxima) gibberellin (ga) 20-oxidase sequence, six different fragments of dioxygenase genes were amplified by polymerase chain reaction from arabidopsis thaliana genomic dna. one of these was used to isolate two different full-length cdna clones, at2301 and at2353, from shoots of the ga-deficient arabidopsis mutant ga1-2. a third, related clone, yap169, was identified in the database of expressed sequence tags. the cdna clones ... | 1995 | 7630935 |
| purification of a trypsin inhibitor (pfti) from pumpkin fruit phloem exudate and isolation of putative trypsin and chymotrypsin inhibitor cdna clones. | the major trypsin inhibitor from pumpkin (cucurbita maxima cv supermarket hybrid) fruit phloem exudate was purified by affinity and reverse phase chromatography. the protein has a molecular weight of approximately 8100 by sds-page and is blocked at the n-terminal serine. following sequencing of a cnbr fragment, 3'- and 5'-race were used to isolate full length cdnas corresponding to a trypsin inhibitor and to two chymotrypsin inhibitors. the three genes are similar, both in their translated and n ... | 1995 | 7662170 |
| three-dimensional solution structure of cucurbita maxima trypsin inhibitor-v determined by nmr spectroscopy. | the solution structure of cucurbita maxima trypsin inhibitor-v (cmti-v), which is also a specific inhibitor of the blood coagulation protein, factor xiia, was determined by 1h nmr spectroscopy in combination with a distance-geometry and simulated annealing algorithm. sequence-specific resonance assignments were made for all the main-chain and most of the side-chain hydrogens. stereospecific assignments were also made for some of the beta-, gamma-, delta-, and epsilon-hydrogens and valine methyl ... | 1995 | 7711040 |
| purification and partial amino-acid sequence of gibberellin 20-oxidase from cucurbita maxima l. endosperm. | gibberellin (ga) 20-oxidase was purified to apparent homogeneity from cucurbita maxima endosperm by fractionated ammonium-sulphate precipitation, gel-filtration chromatography and anion-exchange and hydrophobic-interaction high-performance liquid chromatography (hplc). average purification after the last step was 55-fold with 3.9% of the activity recovered. the purest single fraction was enriched 101-fold with 0.2% overall recovery. apparent relative molecular mass of the enzyme was 45 kda, as d ... | 1994 | 7765793 |
| growth of cucurbita maxima l. plants in the presence of the cycloartenol synthase inhibitor u18666a. | squash, like other cucurbitaceae, have unique sterol profiles that offer an excellent opportunity to examine the relationship between sterol biosynthesis and plant growth. to determine the effect of sterol biosynthesis inhibition on squash growth, cucurbita maxima seedlings with and without cotyledons were subjected to increasing concentrations of the cycloarternol synthase (ec 5.4.99.8) inhibitor 3 beta-(2-diethylaminoethoxy)androstenone (u18666a). inhibition of shoot growth was concentration-d ... | 1995 | 7791534 |
| identification of sulphur-rich proteins which resist rumen degradation and are hydrolysed rapidly by intestinal proteases. | several proteins with high proportions of s-containing essential amino acids were incubated in sheep rumen fluid in vitro and their rate of digestion was examined by sodium dodecyl sulphate-polyacrylamide-gel electrophoresis. the s-rich proteins rice prolamin (10 kda), maize zein (10 kda) and the 3.2 kda pumpkin (cucurbita maxima l.) trypsin inhibitor-1 (cmti-1) were highly resistant to rumen fluid degradation, relative to control proteins of known degradation rate (casein, bovine serum albumin ... | 1994 | 7827006 |
| changing the inhibitory specificity and function of cucurbita maxima trypsin inhibitor-v by site-directed mutagenesis. | cucurbita maxima trypsin inhibitor-v (cmti-v) is also a specific inhibitor of human blood coagulation factor beta-factor xiia. a recombinant version of cmti-v has allowed probing of roles of individual amino acid residues including the reactive site residue, lysine (p1), by site-directed mutagenesis. the k44r showed at least a 5-fold increase in inhibitory activity toward human beta-factor xiia, while there was no change toward bovine trypsin. this result demonstrates that beta-factor-xiia prefe ... | 1995 | 7864895 |
| toxicologic evaluation of acute and subacute oral administration of cucurbita maxima seed extracts to rats and swine. | the extract prepared from dried seeds of cucurbita maxima was administered to rats and pigs. following a single dose or 4 weeks of daily oral administration, the extract produced no changes in serum glucose, urea, creatinine, total protein, uric acid, got, gpt, ldh or blood counts. urine analysis (urea, uric acid, creatinine, total protein, na and k), as well as histopathological investigation, showed no abnormalities. these results taken as a whole indicate that the seeds of c. maxima as used i ... | 1994 | 7967649 |
| organization and characterization of cucurbita phloem lectin genes. | the phloem of pumpkin and squash contains a dimeric chitin-binding lectin called pp2 (phloem protein 2). we have isolated three genomic clones from pumpkin (cucurbita maxima duch.) that encoded pp2. one clone, lambda gpc13-1, contained two pp2 genes that were 99.8% identical over a region of 3055 nucleotides. this conserved region included 1922 bp of 5' non-coding sequence, 844 bp of protein coding sequence (including two introns), and 289 bp of 3' non-coding sequence. to examine the conservatio ... | 1994 | 8000002 |
| new active analogues of cucurbita maxima trypsin inhibitor iii (cmti-iii) modified in the non-contact region. | four new analogues of trypsin inhibitor cmti-iii(3-28) = [desarg1,desval2,desgly29]cmti-iii which was recently shown to be fully active, were synthesized by the solid-phase method. the introduction of glycine in position 9 (peptide 1) and gly-pro-gly (peptide 2) and gly-pro-asn (peptide 3) in the regions 17-19 and 23-25, respectively, did not change the antitrypsin activity of all modified peptides. all of these substitutions are presumed to be outside the trypsin-binding loop as judged from the ... | 1994 | 8003251 |
| analogues of cucurbita maxima trypsin inhibitor iii (cmti-iii) with elastase inhibitory activity. | three new cmti-iii analogues containing the val residue in the reactive site (position 5) were synthesized by the solid-phase method. the analogues displayed an elastase inhibitory activity. it is shown that the removal of the n-terminal arg residue and the introduction of the gly-pro-gln tripeptide in the region 23-25 decreases the antielastase activity by two orders of magnitude. the removal of the disulfide bridge in positions 16-28 and the substitution of ala for cys16 and gly for cys28 decr ... | 1994 | 8060538 |
| expression cloning of a gibberellin 20-oxidase, a multifunctional enzyme involved in gibberellin biosynthesis. | in the biosynthetic pathway to the gibberellins (gas), carbon-20 is removed by oxidation to give the c19-gas, which include the biologically active plant hormones. we report the isolation of a cdna clone encoding a ga 20-oxidase [gibberellin, 2-oxoglutarate:oxygen oxidoreductase (20-hydroxylating, oxidizing) ec 1.14.11.-] by screening a cdna library from developing cotyledons of pumpkin (cucurbita maxima l.) for expression of this enzyme. when mrna from either the cotyledons or the endosperm was ... | 1994 | 8078921 |
| characterization and sequencing of cdna clone encoding the phloem protein pp2 of cucurbita pepo. | direct n-terminal amino acid sequencing of the phloem protein 2 (pp2) from 3-month old cucurbita pepo l. (pumpkin), purified by sds-page and blotted onto pvdf membrane, showed that the protein had a blocked n-terminus. however, after in situ cleavage of the polypeptide in a gel slice by cyanogen bromide, 75 residues of sequences on two cyanogen bromide fragments were determined. an oligonucleotide probe based on this amino acid sequence was used to screen a cdna library, constructed from mrna of ... | 1994 | 8111014 |
| glycine-rich analogues of cucurbita maxima trypsin inhibitor (cmti-iii) substituted by valine in position 27 display relatively low antitrypsin activity. | five new analogues of the trypsin inhibitor cmti-iii were synthesized by the solid-phase method. all analogues containing a valine residue in position 27 and glycine residues in some or all of the positions 9, 11, 14, 17, 19, 29 as well as in two cases a norleucine residue in position 8 displayed association equilibrium constants by 6-7 orders of magnitude lower than the native cmti-iii inhibitor. | 1993 | 8267878 |
| single peptide bond hydrolysis/resynthesis in squash inhibitors of serine proteinases. 1. kinetics and thermodynamics of the interaction between squash inhibitors and bovine beta-trypsin. | the substrate and inhibitory parameters are described for the interaction between cucurbita maxima trypsin inhibitor i (cmti i) and bovine beta-trypsin. the data are fully consistent with the reactive site hypothesis and the standard mechanism proposed for the protein inhibitor-serine proteinase interaction. the second-order association rate constant (k(on)) for the interaction of the intact inhibitor and trypsin is high, above 10(6) m-1 s-1. the same value is only 22-fold lower for the reactive ... | 1994 | 8286341 |
| single peptide bond hydrolysis/resynthesis in squash inhibitors of serine proteinases. 2. limited proteolysis of curcurbita maxima trypsin inhibitor i by pepsin. | porcine pepsin hydrolyzes the leu7-met8 (p2'-p3') peptide bond in cucurbita maxima trypsin inhibitor i (cmti i) in the ph range 2.0-4.8. the reaction proceeds to equilibrium between intact cmti i and its cleaved form. the ph-independent value of the equilibrium constant (khyd0 = 0.78) indicates that both forms of the inhibitor have similar gibbs energies. the ph dependence of this constant shows that the peptide bond hydrolysis does not perturb ionization constants of any preexistent groups. the ... | 1994 | 8286342 |
| purification, characterization, and complete amino acid sequence of a trypsin inhibitor from amaranth (amaranthus hypochondriacus) seeds. | a protein proteinase inhibitor was purified from a seed extract of amaranth (amaranthus hypochondriacus) by precipitation with (nh4)2so4, gel-filtration chromatography, ion-exchange chromatography, and reverse-phase high-performance liquid chromatography. it is a 69-amino acid protein with a high content of valine, arginine, and glutamic acid, but lacking in methionine. the inhibitor has a relative molecular weight of 7400 and an isoelectric point of 7.5. it is a serine proteinase inhibitor that ... | 1993 | 8290633 |
| synthesis, cloning and expression in escherichia coli of a gene coding for the met8-->leu cmti i--a representative of the squash inhibitors of serine proteinases. | a chemically synthesized gene coding for a cucurbita maxima trypsin inhibitor modified at position p'3 (met8-->leu cmti i), i.e. at the third position downstream of the reactive site bond (arg5-ile), was cloned into a derivative of the plasmid paed4 that utilizes a t7 expression system. the gene was expressed in escherichia coli as a fusion protein that accumulates in inclusion bodies. after reduction and cnbr cleavage of the fusion protein followed by oxidative refolding and reverse-phase hplc, ... | 1995 | 8543044 |
| solution conformations of proline rings in proteins studied by nmr spectroscopy. | three different conformations of proline rings in a protein in solution, up, down and twist, have been distinguished, and stereospecific assignments of the pyrrolidine beta-, gamma- and delta-hydrogens have been made on the basis of 1h-1h vicinal coupling constant patterns and intraresidue noes. for all three conformations, interhydrogen distances in the pairs alpha-beta 3, beta 3-gamma 3, beta 2-gamma 2, gamma 2-delta 2, and gamma 3-delta 3 (2.3 a) are shorter than those in the pairs alpha-beta ... | 1995 | 8589600 |
| solution structure and backbone dynamics of recombinant cucurbita maxima trypsin inhibitor-v determined by nmr spectroscopy. | the solution structure of recombinant cucurbita maxima trypsin inhibitor-v (rcmti-v), whose n-terminal is unacetylated and carries an extra glycine residue, was determined by means of two-dimensional (2d) homo and 3d hetero nmr experiments in combination with a distance geometry and simulated annealing algorithm. a total of 927 interproton distances and 123 torsion angle constraints were utilized to generate 18 structures. the root mean squared deviation (rmsd) of the mean structure is 0.53 a fo ... | 1996 | 8634282 |
| antigenotoxic spinasterol from cucurbita maxima flowers. | the antigenotoxic constituent of squash flowers was isolated by solvent partitioning and repeated vacuum liquid chromatography. the micronucleus test, an in vivo method, was used to monitor the antigenotoxicity of the various fractions during the isolation process. isolate sqfwb2d from the chloroform extract of squash flowers is the most antigenotoxic isolate. it decreased the mutagenicity of tetracycline by 64.7% at a dosage of 100 mg/kg mouse. statistical analysis using kruskall wallis one-way ... | 1996 | 8649469 |
| differential modulation of binding loop flexibility and stability by arg50 and arg52 in cucurbita maxima trypsin inhibitor-v deduced by trypsin-catalyzed hydrolysis and nmr spectroscopy. | the side chains of arg50 and arg52 iin cucurbita maxima trypsin inhibitor-v (cmti-v) anchor the binding loop to the scaffold region [cai, m., gong, y., kao, j.l-f., & krishnamoorthi, r. (1995) biochemistry 34, 5201-5211]. the consequences of these hydrogen-bonding and electrostatic interactions on the conformational flexibility and stability of the binding loop were evaluated by trypsin-catalyzed hydrolysis of cmti-v mutants, in which each of the arginines was individually replaced with ala, lys ... | 1996 | 8664268 |
| molecular cloning and photoperiod-regulated expression of gibberellin 20-oxidase from the long-day plant spinach. | spinach (spinacia oleracea l.) is a long-day (ld) rosette plant in which stem growth under ld conditions is mediated by gibberellins (gas). major control points in spinach are the later steps of sequential oxidation and elimination of c-20 of c20-gas. degenerate oligonucleotide primers were used to obtain a polymerase chain reaction product from spinach genomic dna that has a high homology with ga 20-oxidase cdnas from cucurbita maxima l. and arabidopsis thaliana heynh. this polymerase chain rea ... | 1996 | 8742334 |
| molecular cloning and characterization of a cdna encoding the gibberellin biosynthetic enzyme ent-kaurene synthase b from pumpkin (cucurbita maxima l.). | the first committed step in the formation of diterpenoids leading to gibberellin (ga) biosynthesis is the conversion of geranylgeranyl diphosphate (ggdp) to ent-kaurene. ent-kaurene synthase a (ksa) catalyzes the conversion of ggdp to copalyl diphosphate (cdp), which is subsequently converted to ent-kaurene by ent-kaurene synthase b (ksb). a full-length ksb cdna was isolated from developing cotyledons in immature seeds of pumpkin (cucurbita maxima l.). degenerate oligonucleotide primers were des ... | 1996 | 8771778 |
| internal mobility of reactive-site-hydrolyzed recombinant cucurbita maxima trypsin inhibitor-v characterized by nmr spectroscopy: evidence for differential stabilization of newly formed c- and n-termini. | the solution structure and internal dynamics of the reactive-site (lys44-asp45 peptide bond) hydrolyzed form of recombinant cucurbita maxima trypsin inhibitor-v (rcmti-v*) were characterized by the application of two-dimensional 1h-15n nmr methods to the uniformly 15n-labeled protein. the 1h-15n chemical shift correlation spectra of rcmti-v* were assigned, and the chemical shift data were compared with those available for rcmti-v [liu, j., prakash, o., cai, m., gong, y., huang, y., wen, l., wen, ... | 1996 | 8823186 |
| synthesis of a squash-type protease inhibitor by gene engineering and effects of replacements of conserved hydrophobic amino acid residues on its inhibitory activity. | cucurbita maxima trypsin inhibitor i (cmti-i), a member of the squash-type protease inhibitor family, is composed of 29 amino acids and shows strong inhibition of trypsin by its compact structure. to study the structure-function relationship of this inhibitor using protein engineering methods, we constructed an expression system for cmti-i as a fused protein with porcine adenylate kinase (adk). a met residue was introduced into the junction of adk and cmti-i to cleave the fusion protein with cnb ... | 1996 | 9010939 |
| effect of disruption of a cutinase gene (cuta) on virulence and tissue specificity of fusarium solani f. sp. cucurbitae race 2 toward cucurbita maxima and c. moschata. | a 3.9-kb genomic dna fragment from the cucurbit pathogen fusarium solani f. sp. cucurbitae race 2 was cloned. sequence analysis revealed an open reading frame of 690 nucleotides interrupted by a single 51-bp intron. the nucleotide and predicted amino acid sequences showed 92 and 98% identity, respectively, to those of the cuta gene of the pea pathogen f. solani f. sp. pisi. a gene replacement vector was constructed and used to generate cuta- mutants that were detected with a polymerase chain rea ... | 1997 | 9100380 |
| gibberellin biosynthesis from gibberellin a12-aldehyde in endosperm and embryos of marah macrocarpus. | soluble enzyme preparations from embryos and endosperm of marah macrocarpus (previously echinocystis macrocarpa) were incubated with [14c4]gibberellin(ga)12-aldehyde, [14c4]ga12, [14c1] ga9, 2,3-didehydro[14c1]ga9, [14c1]ga20, and [17-13c, 3h]ga5. embryo preparations converted ga12-aldehyde, ga12, and ga9 to ga4 and ga7; 2,3-didehydroga9 to ga7; ga5 to ga3; and ga20 (incompletely) to ga1 and ga60, but not to ga3. endosperm preparations converted ga12-aldehyde and ga12 to ga15, ga24, and ga9, but ... | 1997 | 9112781 |
| [color loss evaluation of a dietary formulation in function of temperature, storage time and packaging]. | color loss of a dietetic formulae based on pumpkin (cucurbita maxima), rice, hen and vegetable oils was evaluated. the product was under an accelerated test at temperatures of 40, 50 and 70 degrees c for a month. the kinetic reaction of color loss (quality loss indicator) was obtained with n = 1 and ea = 11.5 kcal/mol. the product was also stored for sixth months at temperatures of 25, 30, 35 and 40 degrees c using glass containers, laminated and composite cans (non metallic). the color was meas ... | 1996 | 9161461 |
| molecular characterization of a phloem-specific gene encoding the filament protein, phloem protein 1 (pp1), from cucurbita maxima. | sieve elements in the phloem of most angiosperms contain proteinaceous filaments and aggregates called p-protein. in the genus cucurbita, these filaments are composed of two major proteins: pp1, the phloem filament protein, and pp2, the phloem lactin. the gene encoding the phloem filament protein in pumpkin (cucurbita maxima duch.) has been isolated and characterized. nucleotide sequence analysis of the reconstructed gene gpp1 revealed a continuous 2430 bp protein coding sequence, with no intron ... | 1997 | 9263452 |
| phloem sap proteins from cucurbita maxima and ricinus communis have the capacity to traffic cell to cell through plasmodesmata. | in angiosperms, the functional enucleate sieve tube system of the phloem appears to be maintained by the surrounding companion cells. in this study, we tested the hypothesis that polypeptides present within the phloem sap traffic cell to cell from the companion cells, where they are synthesized, into the sieve tube via plasmodesmata. coinjection of fluorescently labeled dextrans along with size-fractionated cucurbita maxima phloem proteins, ranging in size from 10 to 200 kda, as well as injectio ... | 1997 | 9391168 |
| nmr studies of internal dynamics of serine proteinase protein inhibitors: binding region mobilities of intact and reactive-site hydrolyzed cucurbita maxima trypsin inhibitor (cmti)-iii of the squash family and comparison with those of counterparts of cmti-v of the potato i family. | serine proteinase protein inhibitors follow the standard mechanism of inhibition (laskowski m jr, kato i, 1980, annu rev biochem 49:593-626), whereby an enzyme-catalyzed equilibrium between intact (i) and reactive-site hydrolyzed inhibitor (i*) is reached. the hydrolysis constant, khyd, is defined as [i*]/[i]. here, we explore the role of internal dynamics in the resynthesis of the scissile bond by comparing the internal mobility data of intact and cleaved inhibitors belonging to two different f ... | 1998 | 9514268 |
| the ga2 locus of arabidopsis thaliana encodes ent-kaurene synthase of gibberellin biosynthesis. | the ga2 mutant of arabidopsis thaliana is a gibberellin-deficient dwarf. previous biochemical studies have suggested that the ga2 mutant is impaired in the conversion of copalyl diphosphate to ent-kaurene, which is catalyzed by ent-kaurene synthase (ks). overexpression of the previously isolated ks cdna from pumpkin (cucurbita maxima) (cmks) in the ga2 mutant was able to complement the mutant phenotype. a genomic clone coding for ks, atks, was isolated from a. thaliana using cmks cdna as a heter ... | 1998 | 9536043 |
| specificity of human cathepsin g. | a series of tetrapeptide p-nitroanilide substrates of the general formula: suc-ala-ala-pro-aaa-p-nitroanilide was used to map the s1 binding pocket of human cathepsin g. based on the kcat/km parameter, the following order of preference was found: lys=phe>arg=leu>met>nle=nva>ala>asp. thus, the enzyme exhibits clear dual and equal trypsin- and chymotrypsin-like specificities. particularly deleterious were beta-branched side chains of ile and val. the p1 substrate preferences found for cathepsin g ... | 1998 | 9675278 |
| modifications outside the proteinase binding loop in cucurbita maxima trypsin inhibitor iii (cmti-iii) analogues change the binding energy with bovine beta-trypsin. | five 26-peptide analogues of the trypsin inhibitor [pro18]cmti-iii containing leu or tyr in position 7 and val or tyr in position 27: 1 (leu7, tyr27), 2 (tyr7, val27), 3 (tyr7, tyr27), 4 (leu7, val27) and 5 (leu7, ala18, tyr27) were synthesized by the solid-phase method. analogues 1-4 displayed ka with bovine beta-trypsin of the same order of magnitude as the wild cmti-iii inhibitor, whereas for analogue 5, this value was lower by about 3 orders of magnitude. this indicated that for the analogue ... | 1998 | 9781673 |
| the first step of gibberellin biosynthesis in pumpkin is catalyzed by at least two copalyl diphosphate synthases encoded by differentially regulated genes. | the first step in gibberellin biosynthesis is catalyzed by copalyl diphosphate synthase (cps) and ent-kaurene synthase. we have cloned from pumpkin (cucurbita maxima l.) two cdnas, cmcps1 and cmcps2, that each encode a cps. both recombinant fusion cmcps proteins were active in vitro. cps are translocated into plastids and processed by cleavage of transit peptides. for cmcps1 and cmcps2, the putative transit peptides cannot exceed the first 99 and 107 amino acids, respectively, because longer n-t ... | 1998 | 9847116 |
| plant paralog to viral movement protein that potentiates transport of mrna into the phloem. | cmpp16 from cucurbita maxima was cloned and the protein was shown to possess properties similar to those of viral movement proteins. cmpp16 messenger rna (mrna) is present in phloem tissue, whereas protein appears confined to sieve elements (se). microinjection and grafting studies revealed that cmpp16 moves from cell to cell, mediates the transport of sense and antisense rna, and moves together with its mrna into the se of scion tissue. cmpp16 possesses the characteristics that are likely requi ... | 1999 | 9872750 |
| ribosome inactivating protein and lectin from bitter melon (momordica charantia) seeds: sequence comparison with related proteins. | one of the ribosome inactivating proteins (rips), beta-momorcharin, and a lectin were isolated from seeds of the bitter melon momordica charantia (family cucurbitaceae) in accordance with published procedures. both beta-momorcharin and m. charantia lectin were then subjected to amino acid sequencing. beta-momorcharin exhibited considerable homology in sequence to other cucurbitaceae rips, and also to the a chains of abrin and ricin which are type ii (double-chained) rips. the resemblance between ... | 1998 | 9875234 |
| solution structure by nmr of circulin a: a macrocyclic knotted peptide having anti-hiv activity. | the three-dimensional solution structure of circulin a, a 30 residue polypeptide from the african plant chassalia parvifolia, has been determined using two-dimensional 1h-nmr spectroscopy. circulin a was originally identified based upon its inhibition of the cytopathic effects and replication of the human immunodeficiency virus. structural restraints consisting of 369 interproton distances inferred from nuclear overhauser effects, and 21 backbone dihedral and nine chi1 angle restraints from spin ... | 1999 | 9878410 |
| translocation of structural p proteins in the phloem. | phloem-specific proteins (p proteins) are particularly useful markers to investigate long-distance trafficking of macromolecules in plants. in this study, genus-specific molecular probes were used in combination with intergeneric grafts to reveal the presence of a pool of translocatable p protein subunits. immunoblot analyses demonstrated that cucurbita spp p proteins pp1 and pp2 are translocated from cucurbita maxima stocks and accumulate in cucumis sativus scions. cucurbita maxima or cucurbita ... | 1999 | 9878637 |
| high-resolution structures of three new trypsin-squash-inhibitor complexes: a detailed comparison with other trypsins and their complexes. | an anionic trypsin from atlantic salmon and bovine trypsin have been complexed with the squash-seed inhibitors, cmti-i (cucurbita maxima trypsin inhibitor i, p1 arg) and cpti-ii (cucurbita pepo trypsin inhibitor ii, p1 lys). the crystal structures of three such complexes have been determined to 1.5-1.8 a resolution and refined to crystallographic r factors ranging from 17.6 to 19.3%. the two anionic salmon-trypsin complexes (st-cpti and st-cmti) and the bovine-trypsin complex (bt-cpti) have been ... | 1999 | 10089404 |
| analysis of phloem protein patterns from different organs of cucurbita maxima duch. by matrix-assisted laser desorption/ionization time of flight mass spectroscopy combined with sodium dodecyl sulfate polyacrylamide gel electrophoresis. | sieve tubes mediate the long-distance transport of nutrients and signals between source and sink organs of plants. to detect mobile phloem proteins that are differentially distributed in source and sink organs of cucurbita maxima, we used both one-dimensional gel electrophoresis (sds-page) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms). both techniques revealed that phloem protein patterns depend on the sampling site: whilst several proteins were ... | 1999 | 10093900 |
| purification and partial characterization of the pancreatic proteolytic enzymes trypsin, chymotrypsin, and elastase from the chicken. | the purpose of this work was to isolate, purify and partially sequence trypsin, chymotrypsin and elastase from the chicken pancreas. the extraction of the pancreatic zymogens with 0.5 m cacl2 at ph 7.5 for 9 h appeared to be most effective in obtaining maximum recovery of the three enzymes. the sequential cucurbita maxima trypsin inhibitor i/bovine pancreas trypsin inhibitor/soybean trypsin inhibitor affinity chromatography gave the best result for the isolation of trypsin, chymotrypsin and elas ... | 1999 | 10480246 |
| phloem long-distance transport of cmnacp mrna: implications for supracellular regulation in plants. | direct support for the concept that rna molecules circulate throughout the plant, via the phloem, is provided through the characterisation of mrna from phloem sap of mature pumpkin (cucurbita maxima) leaves and stems. one of these mrnas, cmnacp, is a member of the nac domain gene family, some of whose members have been shown to be involved in apical meristem development. in situ rt-pcr analysis revealed the presence of cmnacp rna in the companion cell-sieve element complex of leaf, stem and root ... | 1999 | 10498677 |
| identification and localization of transformed cells in agrobacterium tumefaciens-induced plant tumors | agrobacterium tumefaciens-induced tumors of dicotyledonous plants consist of well-defined vascular bundle-like structures originating from transformed cells. the current view that 25% of the tumor cells are transformed has been re-investigated by using beta-glucuronidase (gus)-gene-containing wild-type bacteria (a281 p35s gus-int). regularly growing stem and leaf tumors showed irregular gus-staining patterns in the different plant species, ricinus communis l., cucurbita maxima l., vicia faba l. ... | 1999 | 10550620 |
| traditional antihelmintic, antiparasitic and repellent uses of plants in central italy. | the uses of 51 plants of marche, abruzzo and latium, distributed in 28 families, are listed here. memories and news of continued use of the plants in these sectors were collected from farmers and shepherds in person (mostly old people). the plants most frequently used as antiparasitics and repellents are juglans regia, lupinus albus, ruta graveolens, fraxinus ornus, datura stramonium, artemisia absinthium, allium cepa, while r. graveolens, cucurbita maxima, a. absinthium, allium sativum are the ... | 1999 | 10624877 |