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analysis of genetic variability of commercial melon cultivars using ssr molecular markers.taking into account the scenario of melon production (cucumis melo l.) in brazil, it is notable the importance of the culture in the fruit market. the preference of the national market is concentrated in the fruits of the yellow type. therefore, it is possible that the genetic base of the commercial cultivars is relatively narrow due to the loss of important genes in the breeding process, and it is possible to introgress external genes for increasing recombination possibilities that generate sou ...201728873211
genome-wide linkage-disequilibrium mapping to the candidate gene level in melon (cucumis melo).cucumis melo is highly diverse for fruit traits providing wide breeding and genetic research opportunities, including genome-wide association (gwa) analysis. we used a collection of 177 accessions representing the two c. melo subspecies and 11 horticultural groups for detailed characterization of fruit traits variation and evaluation of the potential of gwa for trait mapping in melon. through genotyping-by-sequencing, 23,931 informative snps were selected for genome-wide analyses. we found that ...201728852011
dissipation kinetics, pre-harvest residue limits, and hazard quotient assessments of pesticides flubendiamide and fluopicolide in korean melon (cucumis melo l. var. makuwa) grown under regulated conditions in plastic greenhouses.the dissipation kinetics, pre-harvest residue limits, and hazard quotient (hq) assessments of the pesticides flubendiamide and fluopicolide were conducted for korean melon (cucumis melo l. var. makuwa) cultivated at two different sites. a single extraction and cleanup procedure was carried out using acetone (partitioned with dichloromethane) and amino solid-phase extraction cartridges, respectively. residue analysis was performed by hplc with ultraviolet detection. both pesticides showed excelle ...201728799066
proteogenomics data for deciphering frankia coriariae interactions with root exudates from three host plants.frankia coriariae bmg5.1 cells were incubated with root exudates derived from compatible (coriaria myrtifolia), incompatible (alnus glutinosa) and non-actinorhizal (cucumis melo) host plants. bacteria cells and their exoproteomes were analyzed by high-throughput proteomics using a q-exactive hf high resolution tandem mass spectrometer incorporating an ultra-high-field orbitrap analyzer. ms/ms spectra were assigned with two protein sequence databases derived from the closely-related genomes from ...201728765834
genome-wide identification of hami melon mirnas with putative roles during fruit development.micrornas represent a family of small endogenous, non-coding rnas that play critical regulatory roles in plant growth, development, and environmental stress responses. hami melon is famous for its attractive flavor and excellent nutritional value, however, the mechanisms underlying the fruit development and ripening remains largely unknown. here, we performed small rna sequencing to investigate the roles of mirnas during hami melon fruit development. two batches of flesh samples were collected a ...201728742088
genome-wide analysis of simple sequence repeats in bitter gourd (momordica charantia).bitter gourd (momordica charantia) is widely cultivated as a vegetable and medicinal herb in many asian and african countries. after the sequencing of the cucumber (cucumis sativus), watermelon (citrullus lanatus), and melon (cucumis melo) genomes, bitter gourd became the fourth cucurbit species whose whole genome was sequenced. however, a comprehensive analysis of simple sequence repeats (ssrs) in bitter gourd, including a comparison with the three aforementioned cucurbit species has not yet be ...201728690629
mapping and characterization of the fefe gene that controls iron uptake in melon (cucumis melo l.).iron (fe) deficiency in plants limits crop growth and productivity. molecular mechanisms that plants use to sense and respond to fe deficiency by coordinated expression of fe-uptake genes are not fully understood. the c940-fe chlorotic melon (cucumis melo) mutant known as fefe is unable to upregulate fe-uptake genes, however, the fefe gene had not been identified. in this study, we used two f2 mapping populations to map and identify the fefe gene as bhlh38, a homolog of subgroup ib bhlh genes fr ...201728659950
mapping and preliminary analysis of aborted microspores (ams) as the candidate gene underlying the male sterility (ms-5) mutant in melon (cucumis melo l.).melon is an important agricultural and economic vegetable crop worldwide. the genetic male sterility mutant (ms-5) has a recessive nuclear gene that controls the male sterility germplasm. male sterility could reduce the cost of f1 seed production in melon, but heterozygous fertile plants should be removed before pollination. in this study, bulked segregant analysis combined with specific length amplified fragment sequencing was applied to map the single nuclear male sterility recessive gene. a 3 ...201728611814
the quest for epigenetic regulation underlying unisexual flower development in cucumis melo.melon (cucumis melo) is an important vegetable crop from the cucurbitaceae family and a reference model specie for sex determination, fruit ripening and vascular fluxes studies. nevertheless, the nature and role of its epigenome in gene expression regulation and more specifically in sex determination remains largely unknown.201728592995
influence of environmental factors on cucumis melo l. var. agrestis naud. seed germination and seedling emergence.cucumis melo l. var. agrestis naud. (field muskmelon) is an annual invasive weed in many parts of asia. however, there is very little available information about the germination and emergence of this species. therefore, laboratory experiments were conducted to evaluate the effects of light, temperature, salt stress, osmotic stress, ph, and depth of planting on field muskmelon germination and seedling emergence. light had no effect on seed germination, and the seeds germinated at a wide range of ...201728570590
dissipation pattern and risk quotients assessment of amisulbrom in korean melon cultivated in plastic house conditions.amisulbrom formulated as suspension concentrate was applied at the rate recommended for korean melon to determine the dissipation pattern (at two different sites), the pre-harvest residue limit (phrl), and risk assessments. samples collected over 10 days were extracted using liquid-liquid extraction (lle) and cleaned up with solid-phase extraction (spe) florisil cartridge. residual concentrations were determined using liquid chromatography-ultraviolet detector (lc-uvd) and confirmed by liquid ch ...201728560618
green synthesis of highly stable carbon nanodots and their photocatalytic performance.the present study reports a novel, facile, biosynthesis route for the synthesis of carbon nanodots (cds) with an approximate quantum yield of 38.5%, using musk melon extract as a naturally derived-precursor material. the synthesis of cds was established by using ultraviolet-visible (uv-vis) spectroscopy, dynamic light scattering, photoluminescence spectroscopy, x-ray diffraction, transmission electron microscopy and fourier transform infrared (ftir) spectroscopy. the as-prepared cds possess an e ...201728530182
structure of eif4e in complex with an eif4g peptide supports a universal bipartite binding mode for protein translation.the association-dissociation of the cap-binding protein eukaryotic translation initiation factor 4e (eif4e) with eif4g is a key control step in eukaryotic translation. the paradigm on the eif4e-eif4g interaction states that eif4g binds to the dorsal surface of eif4e through a single canonical alpha-helical motif, while metazoan eif4e-binding proteins (m4e-bps) advantageously compete against eif4g via bimodal interactions involving this canonical motif and a second noncanonical motif of the eif4e ...201728522457
host plant compatibility shapes the proteogenome of frankia coriariae.molecular signaling networks in the actinorhizal rhizosphere select host-compatible frankia strains, trigger the infection process and eventually the genesis of nitrogen-fixing nodules. the molecular triggers involved remain difficult to ascertain. root exudates (re) are highly dynamic substrates that play key roles in establishing the rhizosphere microbiome. re are known to induce the secretion by rhizobia of nod factors, polysaccharides, and other proteins in the case of legume symbiosis. next ...201728512450
ethqv6.3 is involved in melon climacteric fruit ripening and is encoded by a nac domain transcription factor.fruit ripening is divided into climacteric and non-climacteric types depending on the presence or absence of a transient rise in respiration rate and the production of autocatalytic ethylene. melon is ideal for the study of fruit ripening, as both climacteric and non-climacteric varieties exist. two introgressions of the non-climacteric accession pi 161375, encompassed in the qtls ethqb3.5 and ethqv6.3, into the non-climacteric 'piel de sapo' background are able to induce climacteric ripening in ...201728493311
pectin from muskmelon (cucumis melo var. reticulatus) peels: extraction optimization and physicochemical properties.pectin derived from plant waste sources is currently focused as an economical and eco-friendly approach. optimization of pectin extraction from muskmelon peel by response surface methodology (rsm) was investigated in this study. box-behnken design (bbd) was used to identify the optimal level of the extraction variables such as time, ph and temperature. a second-order model equation for pectin extraction was obtained from multiple regression analysis of experimental data with the correlation coef ...201728452020
de novo comparative transcriptome analysis of genes involved in fruit morphology of pumpkin cultivars with extreme size difference and development of est-ssr markers.the genetic basis of fruit size and shape was investigated for the first time in cucurbita species and genetic loci associated with fruit morphology have been identified. although extensive genomic resources are available at present for tomato (solanum lycopersicum), cucumber (cucumis sativus), melon (cucumis melo) and watermelon (citrullus lanatus), genomic databases for cucurbita species are limited. recently, our group reported the generation of pumpkin (cucurbita pepo) transcriptome database ...201728435133
three phase partitioning, a scalable method for the purification and recovery of cucumisin, a milk-clotting enzyme, from the juice of cucumis melo var. reticulatus.cucumisin [ec 3.4.21.25] was first purified from cucumis melo var. reticulatus juice by three-phase partitioning (tpp). optimum purification parameters of the tpp system were determined as 60% ammonium sulfate saturation with 1.0:1.25 ratio of crude extract: t-butanol at ph and temperature of 8.0 and 20°c, respectively. cucumisin was purified with 4.61 purification fold and 156% activity recovery. the molecular weight of the recovered cucumisin was determined as 68.4kda and its isoelectric point ...201728428129
multitrait analysis of fresh-cut cantaloupe melon enables discrimination between storage times and temperatures and identifies potential markers for quality assessments.fresh-cut cantaloupe melon is valued for its aroma but is highly perishable. temperature of storage (typically 0-5°c) is critical for maintaining fresh-cut melon quality, but often reaches 10°c during transportation and in retail outlets. a comparison amongst 0, 5 and 10°c storage temperatures for fresh-cut melon over 14days reveals that storage at 0°c is optimal for avoiding increases in microbial load and loss of vitamin c especially at later time points. however, higher temperatures maintain ...201828958522
melonet-db, a grand rna-seq gene expression atlas in melon (cucumis melo l.).melon (cucumis melo l.) is an important cucurbitaceae crop produced worldwide, exhibiting wide genetic variations and comprising both climacteric and non-climacteric fruit types. the muskmelon cultivar "'earl's favorite harukei-3 (harukei-3)"' known for its sweetness and rich aroma is used for breeding of high-grade muskmelon in japan. we conducted rna sequencing (rna-seq) transcriptome studies in 30 different tissues of the 'harukei-3' melon. these included root, stems, leaves, flowers, regener ...201829216378
construction of a genome-anchored, high-density genetic map for melon (cucumis melo l.) and identification of fusarium oxysporum f. sp. melonis race 1 resistance qtl.four qtls and an epistatic interaction were associated with disease severity in response to inoculation with fusarium oxysporum f. sp. melonis race 1 in a recombinant inbred line population of melon. the usda cucumis melo inbred line, mr-1, harbors a wealth of alleles associated with resistance to several major diseases of melon, including powdery mildew, downy mildew, alternaria leaf blight, and fusarium wilt. mr-1 was crossed to an israeli cultivar, ananas yok'neam, which is susceptible to all ...201829372283
fruit flesh volatile and carotenoid profile analysis within the cucumis melo l. species reveals unexploited variability for future genetic breeding.aroma profile and carotenoids content of melon flesh are two important aspects influencing the quality of this fruit that have been characterized using only selected genotypes. however, the extant variability of the whole species remains unknown.201829369359
double function hydroperoxide lyases/epoxyalcohol synthases (cyp74c) of higher plants: identification and conversion into allene oxide synthases by site-directed mutagenesis.the cyp74c subfamily of fatty acid hydroperoxide transforming enzymes includes hydroperoxide lyases (hpls) and allene oxide synthases (aoss). this work reports a new facet of the putative cyp74c hpls. initially, we found that the recombinant cyp74c13_mt (medicago truncatula) behaved predominantly as the epoxyalcohol synthase (eas) towards the 9(s)-hydroperoxide of linoleic acid. at the same time, the cyp74c13_mt mostly possessed the hpl activity towards the 13(s)-hydroperoxides of linoleic and α ...201829325723
overexpression of the tonoplast sugar transporter cmtst2 in melon fruit increases sugar accumulation.fruits are an important part of the human diet and sugar content is a major criterion used to evaluate fruit quality. melon fruit accumulate high sugar concentrations during their development; however, the mechanism through which these sugars are transported into the vacuoles of fruit cells for storage remains unclear. in this study, three tonoplast sugar transporters (tsts), cmtst1, cmtst2, and cmtst3, were isolated from melon plants. analysis of subcellular localization revealed that all these ...201829309616
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