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proteogenomics data for deciphering frankia coriariae interactions with root exudates from three host plants.frankia coriariae bmg5.1 cells were incubated with root exudates derived from compatible (coriaria myrtifolia), incompatible (alnus glutinosa) and non-actinorhizal (cucumis melo) host plants. bacteria cells and their exoproteomes were analyzed by high-throughput proteomics using a q-exactive hf high resolution tandem mass spectrometer incorporating an ultra-high-field orbitrap analyzer. ms/ms spectra were assigned with two protein sequence databases derived from the closely-related genomes from ...201728765834
chemometric evaluation of the volatile profile of probiotic melon and probiotic cashew juice.the aim of this study was to evaluate the influence of the lactic acid fermentation on volatile compounds of melon and cashew apple juices. the effect of the fermentation processing on the volatile profile of probiotic juices was assessed by hs-spme/gc-ms coupled to chemometrics with 67.9% and 81.0% of the variance in the first principal component for melon and cashew juices, respectively. the lactobacillus casei fermentation imparted a reduction of ethyl butanoate, ethyl-2-methylbutirate, and e ...201728784506
dissipation kinetics, pre-harvest residue limits, and hazard quotient assessments of pesticides flubendiamide and fluopicolide in korean melon (cucumis melo l. var. makuwa) grown under regulated conditions in plastic greenhouses.the dissipation kinetics, pre-harvest residue limits, and hazard quotient (hq) assessments of the pesticides flubendiamide and fluopicolide were conducted for korean melon (cucumis melo l. var. makuwa) cultivated at two different sites. a single extraction and cleanup procedure was carried out using acetone (partitioned with dichloromethane) and amino solid-phase extraction cartridges, respectively. residue analysis was performed by hplc with ultraviolet detection. both pesticides showed excelle ...201728799066
pathological interrelations of soil-borne diseases in cucurbits caused by fusarium species and meloidogyne incognita.pathological interrelations of two soil-borne diseases in cucurbits (watermelon, oriental melon, shintosa and cucumber) caused by fusarium isolates (fi) and the root-knot nematode (rkn), meloidogyne incognita were characterized by the fusarium disease severity index (di), rkn gall index (gi) and eggmass index (ei) in inoculation tests using fi and rkn. virulence of fi as determined by di at 4 weeks after inoculation was mostly in the higher order of fusarium proliferatum f6, f5 and fusarium oxys ...201728811758
genome-wide linkage-disequilibrium mapping to the candidate gene level in melon (cucumis melo).cucumis melo is highly diverse for fruit traits providing wide breeding and genetic research opportunities, including genome-wide association (gwa) analysis. we used a collection of 177 accessions representing the two c. melo subspecies and 11 horticultural groups for detailed characterization of fruit traits variation and evaluation of the potential of gwa for trait mapping in melon. through genotyping-by-sequencing, 23,931 informative snps were selected for genome-wide analyses. we found that ...201728852011
analysis of genetic variability of commercial melon cultivars using ssr molecular markers.taking into account the scenario of melon production (cucumis melo l.) in brazil, it is notable the importance of the culture in the fruit market. the preference of the national market is concentrated in the fruits of the yellow type. therefore, it is possible that the genetic base of the commercial cultivars is relatively narrow due to the loss of important genes in the breeding process, and it is possible to introgress external genes for increasing recombination possibilities that generate sou ...201728873211
genotypic differences in physiological and biochemical responses to salinity stress in melon (cucumis melo l.) plants: prospects for selection of salt tolerant landraces.melon (cucumis melo l.) is one of the most important horticultural crops in iran often cultivated in arid and semiarid regions of the country with salinity problems. the objective of this work was to better understand the mechanisms of physiological and biochemical responses to salinity stress of five iranian melon landraces "samsuri", "kashan", "khatouni", "suski-e-sabz", and "ghobadlu" from different geographical origins, and "galia" f1 cultivar. plants were grown under greenhouse conditions a ...201728938176
multitrait analysis of fresh-cut cantaloupe melon enables discrimination between storage times and temperatures and identifies potential markers for quality assessments.fresh-cut cantaloupe melon is valued for its aroma but is highly perishable. temperature of storage (typically 0-5°c) is critical for maintaining fresh-cut melon quality, but often reaches 10°c during transportation and in retail outlets. a comparison amongst 0, 5 and 10°c storage temperatures for fresh-cut melon over 14days reveals that storage at 0°c is optimal for avoiding increases in microbial load and loss of vitamin c especially at later time points. however, higher temperatures maintain ...201828958522
cefotaxime and benzyladenine improve melon regeneration.worldwidely cultivated, melon is commercially an important fruit crop, as it is in iran.201628959319
validation of a landscape-based model for whitefly spread of the cucurbit yellow stunting disorder virus to fall melons.the cucurbit yellow stunting disorder virus (cysdv) transmitted by bemisia tabaci (gennadius) (hemiptera: aleyrodidae) has caused significant reductions in fall melon (cucumis melo l.) yields in yuma county, arizona. in a recent landscape-based study, we found evidence that cotton and spring melon fields increased abundance of b. tabaci and spread of cysdv infection in fall melon fields. here, we show that a statistical model derived from data collected in 2011-2012 and based on areas of cotton ...201728961816
chromatographic determination, decline dynamic and risk assessment of sulfoxaflor in asian pear and oriental melon.the dissipation pattern of sulfoxaflor in asian pear cultivated in an open field conditions and in oriental melon grown under plastic house conditions was each studied in two different locations. residues in field-treated samples were determined using liquid chromatography coupled with an ultraviolet detector and confirmed by liquid chromatography-tandem mass spectrometry. a calibration curve for sulfoxaflor was linear over the concentration range 0.1-5.0 mg/l, with a coefficient of determinatio ...201728975659
a re-sequencing-based ultra-dense genetic map reveals a gummy stem blight resistance-associated gene in cucumis melo.the melon (cucumis melo) genome and genetic maps with hundreds to thousands of single nucleotide polymorphism markers were recently released. however, a high-resolution genetic map was lacking. gummy stem blight (gsb) is a destructive disease responsible for considerable economic losses during melon production. we herein describe the development of an ultra-dense genetic map consisting of 12,932 recombination bin markers covering 1,818 cm, with an average distance of 0.17 cm between adjacent tag ...201728985339
qtl analyses in multiple populations employed for the fine mapping and identification of candidate genes at a locus affecting sugar accumulation in melon (cucumis melo l.).sugar content is the major determinant of both fruit quality and consumer acceptance in melon (cucumis melo l), and is a primary target for crop improvement. near-isogenic lines (nils) derived from the intraspecific cross between a "piel de sapo" (ps) type and the exotic cultivar "songwhan charmi" (sc), and several populations generated from the cross of ps × ames 24294 ("trigonus"), a wild melon, were used to identify qtl related to sugar and organic acid composition. seventy-eight qtl were det ...201729018473
antipyretic effects of hydro-methanol extract of melia azedarach linn. seeds and cucumis melo linn. seeds in experimental rabbits.to investigate the antipyretic activity of hydro-methanol extract of melia azedarach linn. (hmema) seeds and cucumis melo linn. (hmecm) seeds in experimental animals. baker's yeast was used to induce fever in rabbits which were divided into six groups. the animal groups were thereafter administered distilled water (control), paracetamol (reference standard, 150mg/kg), hmema (250mg/kg), hmema (500mg/kg), hmecm (250mg/kg) and hmecm (500mg/kg) respectively. hmema and hmecm were also phytochemically ...201729039323
hexanoic acid treatment prevents systemic mnsv movement in cucumis melo plants by priming callose deposition correlating sa and opda accumulation.unlike fungal and bacterial diseases, no direct method is available to control viral diseases. the use of resistance-inducing compounds can be an alternative strategy for plant viruses. here we studied the basal response of melon to melon necrotic spot virus (mnsv) and demonstrated the efficacy of hexanoic acid (hx) priming, which prevents the virus from systemically spreading. we analysed callose deposition and the hormonal profile and gene expression at the whole plant level. this allowed us t ...201729104580
volatile emerging contaminants in melon fruits, analysed by hs-spme-gc-ms.the aim of this research was to develop and validate a headspace-solid phase micro-extraction-gas chromatography-mass spectrometry (hs-spme-gc-ms) method for the determination of volatile emerging contaminants in fruit. the method showed good precision (rsd ≤ 14%) and satisfactory recoveries (99.1-101.7%) and lod and loq values ranging between 0.011-0.033 μg kg-1 and 0.037-0.098 μg kg-1, respectively. the method was applied to investigate the content of volatile emerging contaminants in two vari ...201729111874
glabrous (cmgl) encodes a hd-zip iv transcription factor playing roles in multicellular trichome initiation in melon.map-based cloning identified cmgl that encodes a hd-zip type iv transcription factor that controls multicellular trichome initiation in melon. trichomes are small hairs covering the aerial parts of plants that originate from the epidermal cells, which can protect plants against the damage by insects and pathogens. the regulatory pathway of unicellular trichomes has been well studied in the model plant arabidopsis. little is known about the genetic control and regulation of trichome development i ...201729147724
melonet-db, a grand rna-seq gene expression atlas in melon (cucumis melo l.).melon (cucumis melo l.) is an important cucurbitaceae crop produced worldwide, exhibiting wide genetic variations and comprising both climacteric and non-climacteric fruit types. the muskmelon cultivar "'earl's favorite harukei-3 (harukei-3)"' known for its sweetness and rich aroma is used for breeding of high-grade muskmelon in japan. we conducted rna sequencing (rna-seq) transcriptome studies in 30 different tissues of the 'harukei-3' melon. these included root, stems, leaves, flowers, regener ...201829216378
somatic embryogenesis of muskmelon (cucumis melo l.) and genetic stability assessment of regenerants using flow cytometry and issr markers.a new protocol for in vitro regeneration through direct somatic embryogenesis for two muskmelon cultivars (cucumis melo l., "mashhadi" and "eivanaki") is reported. somatic embryos were obtained culturing 4- and 8-day-old cotyledons, seeds, and hypocotyls on murashige and skoog medium supplemented with three different hormonal combinations never tested so far for melon (naphthoxyacetic acid (noa) + thidiazuron (tdz), noa + 6-banzylaminopurine (bap), and 2,4-dichlorophenoxyacetic acid (2,4-d) + n- ...201729248969
development of machine-vision system for gap inspection of muskmelon grafted seedlings.grafting robots have been developed in the world, but some auxiliary works such as gap-inspecting for grafted seedlings still need to be done by human. an machine-vision system of gap inspection for grafted muskmelon seedlings was developed in this study. the image acquiring system consists of a ccd camera, a lens and a front white lighting source. the image of inspected gap was processed and analyzed by software of halcon 12.0. the recognition algorithm for the system is based on principle of d ...201729267293
overexpression of the tonoplast sugar transporter cmtst2 in melon fruit increases sugar accumulation.fruits are an important part of the human diet and sugar content is a major criterion used to evaluate fruit quality. melon fruit accumulate high sugar concentrations during their development; however, the mechanism through which these sugars are transported into the vacuoles of fruit cells for storage remains unclear. in this study, three tonoplast sugar transporters (tsts), cmtst1, cmtst2, and cmtst3, were isolated from melon plants. analysis of subcellular localization revealed that all these ...201829309616
double function hydroperoxide lyases/epoxyalcohol synthases (cyp74c) of higher plants: identification and conversion into allene oxide synthases by site-directed mutagenesis.the cyp74c subfamily of fatty acid hydroperoxide transforming enzymes includes hydroperoxide lyases (hpls) and allene oxide synthases (aoss). this work reports a new facet of the putative cyp74c hpls. initially, we found that the recombinant cyp74c13_mt (medicago truncatula) behaved predominantly as the epoxyalcohol synthase (eas) towards the 9(s)-hydroperoxide of linoleic acid. at the same time, the cyp74c13_mt mostly possessed the hpl activity towards the 13(s)-hydroperoxides of linoleic and α ...201829325723
fruit flesh volatile and carotenoid profile analysis within the cucumis melo l. species reveals unexploited variability for future genetic breeding.aroma profile and carotenoids content of melon flesh are two important aspects influencing the quality of this fruit that have been characterized using only selected genotypes. however, the extant variability of the whole species remains unknown.201829369359
construction of a genome-anchored, high-density genetic map for melon (cucumis melo l.) and identification of fusarium oxysporum f. sp. melonis race 1 resistance qtl.four qtls and an epistatic interaction were associated with disease severity in response to inoculation with fusarium oxysporum f. sp. melonis race 1 in a recombinant inbred line population of melon. the usda cucumis melo inbred line, mr-1, harbors a wealth of alleles associated with resistance to several major diseases of melon, including powdery mildew, downy mildew, alternaria leaf blight, and fusarium wilt. mr-1 was crossed to an israeli cultivar, ananas yok'neam, which is susceptible to all ...201829372283
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