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effect of cotton cultivar on performance of aphis gossypii (homoptera: aphididae) in iran.cotton aphids, aphis gossypii glover (homoptera: aphididae), obtained from cotton, gossypium hirsutum l., fields in the gorgan region of northern iran, were colonized on 'varamin' cotton plants in a growth chamber. the development, survivorship, and life table parameters of the cotton aphid were evaluated at 27.5 +/- 1 degrees c, 65 +/- 10% rh, and aphotoperiod of 14:10 (l:d) h of artificial light on five commonly growing cotton cultivars: varamin, 'sealand' (relatively resistant cultivar), 'bak ...200617066818
selecting for efficacy of bollgard cotton cultivars against various lepidoptera using forward breeding techniques.studies during the past 5 yr have shown that the overall level of protein (cry1ac) produced from the cry1ac transgene (monsanto co., st. louis, mo) differ among commercial bollgard cotton, gossypium hirsutum l., cultivars. these differences between cultivars are under genetic control and have been correlated with efficacy of certain lepidopteran pests. previous studies have shown that the parental background (i.e., non-cry1ac conventional cultivar) has a significant influence on the amount of cr ...200617066820
evaluation of source leaf responses to water-deficit stresses in cotton using a novel stress bioassay.water-deficit stresses preferentially reduce shoot growth, thereby disrupting the flow of carbohydrates from source leaves to the developing sinks. here, we use a novel stress bioassay to dissect responses of field and greenhouse-grown cotton (gossypium hirsutum) source leaves to water-deficit stresses. fifth main stem leaf samples were harvested at sunrise and subjected to a prolonged elevated respiratory demand in the dark. sucrose levels are lower in nonstressed cotton at sunrise compared to ...200717071650
[cloning of ghaqp1 gene and its specific expression during ovule development in cotton].plant aquaporins, belonging to the mip superfamily, are a series of transmembrane proteins that facilitate water transport through cell membranes. in this study, a cdna clone encoding the pip1-like protein was isolated from cotton (gossypium hirsutum) cdna libraries, and designated as ghaqp1 (fig.1). we also isolated the ghaqp1 gene from cotton genome by pcr. the gene is 2,096 bp in length, including an open reading frame (orf) and 5'-/3'-untranslated regions (utr). it contains two introns in it ...200617075177
[isolation by suppression-subtractive hybridization of genes preferentially expressed during early and late fiber development stages in cotton].as a main natural fiber source, cotton plays an important role in human life. to identify genes preferentially expressed during early and late cotton fiber development, we constructed two fiber subtracted libraries on the basis of pcr-selected subtraction using a pool of nonfiber tissues as the same driver and 10 days postanthesis (dpa) and 20 dpa fiber cells as testers, respectively. through differential screening, 292 clones in both libraries were identified as being preferentially expressed d ...200617086983
[structural-functional organization of chloroplasts in leaves of xantha-702 mutant of gossypium hirsutum l].for cotton mutant xantha (gossypium hirsutum l.), it has been established that synthesis of 5-aminolevulinic acid was blocked in the light. in the light this mutant accumulates chlorophyll by 30 times lower as compared to the parent type. in mutant xantha, a very few pigment-protein complexes of ps-i and ps-ii are formed in chloroplasts, and formation of membrane system in these is blocked at the early stages, in most cases, at the stage of bubbles and single short thylakoids. functional activit ...200617087145
molecular characterization and expression analysis of nine cotton ghef1a genes encoding translation elongation factor 1a.the translation elongation factor 1a, eef1a, plays an important role in protein synthesis, catalyzing the binding of aminoacyl-trna to the a-site of the ribosome by a gtp-dependent mechanism. to investigate the role of eef1a for protein synthesis in cotton fiber development, nine different cdna clones encoding eukaryotic translation elongation factor 1a were isolated from cotton (gossypium hirsutum) fiber cdna libraries. the isolated genes (cdnas) were designated cotton elongation factor 1a gene ...200717107762
enhanced degradation of atrazine under field conditions correlates with a loss of weed control in the glasshouse.enhanced degradation of atrazine has been reported in the literature, indicating the potential for reduced residual weed control with this herbicide. experiments were conducted to determine the field dissipation of atrazine in three cropping systems: continuous zea mays l. (cc) receiving atrazine applications each year, gossypium hirsutum l.-z. mays rotation (ccr) receiving applications of atrazine once every 2 years and a no atrazine history soil (nah). subsequent laboratory and greenhouse expe ...200717115404
xenia effect on seed and embryo size in cotton (gossypium hirsutum l.).the term xenia was coined to describe the effect of foreign pollen on the development and characters of the seed. to study its importance and consequences for various seed traits in cotton (gossypium hirsutum l.), the effect of pollen genotype on seed and embryo weight was studied with seeds from 15 f1 hybrids. cross-fertilization changed seed weight by up to 7.0% in relation to self-fertilization. xenia effect significantly increased embryo weight of cross-fertilized seeds, by up to 14.4% in co ...200617132897
disease resistance conferred by the expression of a gene encoding a synthetic peptide in transgenic cotton (gossypium hirsutum l.) plants.fertile, transgenic cotton plants expressing the synthetic antimicrobial peptide, d4e1, were produced through agrobacterium-mediated transformation. pcr products and southern blots confirmed integration of the d4e1 gene, while rt-pcr of cotton rna confirmed the presence of d4e1 transcripts. in vitro assays with crude leaf protein extracts from t0 and t1 plants confirmed that d4e1 was expressed at sufficient levels to inhibit the growth of fusarium verticillioides and verticillium dahliae compare ...200517147626
genetics of resistance to cotton leaf curl disease in gossypium hirsutum l. under field conditions.one hundred and forty two cotton germplasm lines were screened for cotton leaf curl virus symptoms in field evaluations during 2003, 2004, and 2005. fifty cross combinations involving 30 of these lines classified resistant or susceptible were used for inheritance study of the disease. all the f(1) plants of crosses involving resistant x resistant, resistant x susceptible, and susceptible x resistant parents were resistant, indicating dominant expression of the disease resistance and there were n ...200717159229
role of pantoea agglomerans in opportunistic bacterial seed and boll rot of cotton (gossypium hirsutum) grown in the field.to investigate the aetiology of seed and boll rot of cotton grown in south carolina (sc).200717184328
cadherin-based resistance to bacillus thuringiensis cotton in hybrid strains of pink bollworm: fitness costs and incomplete resistance.recessive resistance to bacillus thuringiensis (bt) cotton, gossypium hirsutum l., in laboratory-selected strains of pink bollworm, pectinophora gossypiella (saunders), is associated with three resistance alleles (r1, r2, and r3) of a cadherin gene. previous experiments based on measurement of fitness components in bt-resistant and bt-susceptible strains revealed that fitness costs and incomplete resistance are associated with resistance. here, we used two hybrid strains of pink bollworm, each c ...200617195656
modeling the impact of alternative hosts on helicoverpa zea adaptation to bollgard cotton.for highly polyphagous cotton, gossypium hirsutum l., pests such as helicoverpa zea (boddie), a substantial portion of the larval population develops on noncotton alternative hosts. these noncotton hosts potentially provide a natural refuge for h. zea, thereby slowing the evolution of resistance to the bacillus thuringiensis berliner (bt)-derived cry1ac protein present in bollgard cotton. here, we demonstrate how the measured contribution of such alternative hosts can be included in estimating t ...200617195681
high-level resistance to bacillus thuringiensis toxin crylac and cadherin genotype in pink bollworm.resistance to transgenic cotton, gossypium hirsutum l., producing bacillus thuringiensis (bt) toxin cry1ac is linked with three recessive alleles of a cadherin gene in laboratory-selected strains of pink bollworm, pectinophora gossypiella (saunders), a major cotton pest. here, we analyzed a strain (mov97-r) with a high frequency of cadherin resistance alleles, a high frequency of resistance to 10 microg of cry1ac per milliliter of diet, and an intermediate frequency of resistance to 1000 microg ...200617195682
a rapid single-step multiplex method for discriminating between trichogramma (hymenoptera: trichogrammatidae) species in australia.inaccurate species identification confounds insect ecological studies. examining aspects of trichogramma ecology pertinent to the novel insect resistance management strategy for future transgenic cotton, gossypium hirsutum l., production in the ord river irrigation area (oria) of western australia required accurate differentiation between morphologically similar trichogramma species. established molecular diagnostic methods for trichogramma identification use species-specific sequence difference ...200617195685
molecular cloning and expression analysis of a ranbp2 zinc finger protein gene in upland cotton (gossypium hirsutum l.).gossypol is an important resistant substance of gossypium, and its storage organ is pigment gland. although, the relationship between gossypol and pigment gland has been studied for a long time, the development mechanism of pigment gland has not been revealed up to now in molecular perspective. on the basis of differentially expressed cdnas fragments at the stage of the cotton gland development using suppression subtractive hybridization (ssh), the complete cdna sequence of a novel ranbp2 zinc f ...200717223018
identification and characterization of the novel gene ghdbp2 encoding a dre-binding protein from cotton (gossypium hirsutum).a cdna encoding one novel dre-binding protein, ghdbp2, was isolated from cotton seedlings. it is classified into the a-6 group of dreb subfamily based on multiple sequence alignment and phylogenetic characterization. using semi-quantitative rt-pcr, we found that the ghdbp2 transcripts were greatly induced by drought, nacl, low temperature and aba treatments in cotton cotyledons. the dna-binding properties of ghdbp2 were analyzed by electrophoretic mobility shift assay (emsa), showing that ghdbp2 ...200817224201
centromere orientation of quadrivalents of heterozygous translocations and an autoploid of gossypium hirsutum l.cytological observations of quadrivalents of heterozygous translocations in gossypium hirsutum l. demonstrate that, in addition to alternate-1 and alternate-2 orientations, a third alternate orientation (alternate-3), which occurs as a three-dimensional, v-type configuration, can be identified.-two additional types of disjunctions, the centromere orientations of which are rotational modifications of either adjacent or alternate configurations, were also observed in quadrivalents of a translocati ...198317246172
transmission of duplication-deficiencies from cotton translocations is unrelated to map lengths of the unbalanced segments.adjacent-1 duplication-deficiencies (dp-dfs) are readily recovered from most heterozygous translocations in gossypium hirsutum l., but frequencies of specific cytotypes differ widely in progenies from heterozygote (female symbol) x standard crosses. surprisingly, these frequencies seem to be unrelated to the primary (postmeiotic) frequencies predicted by metaphase i configurations or to the proportion of the chromosome arm that is duplicate or deficient. deficiencies and duplications from differ ...198717246386
the transmission of marker genes in intraspecific backcrosses of gossypium hirsutum l. 195217247397
viable deficiency-duplications from a translocation in gossypium hirsutum. 195217247415
the genetics of flowering response in cotton. i. fruiting behavior of gossypium hirsutum var. marie-galante in a cross with a variety of cultivated american upland cotton. 195717247711
the genetics of flowering response in cotton. iii. fruiting behavior of gossypium hirsutum race latifolium in a cross with a variety of cultivated american upland cotton. 196117248048
the genetics of flowering response in cotton. iv. quantitative analysis of photoperiodism of texas 86, gossypium hirsutum race latifolium, in a cross with an inbred line of cultivated american upland cotton. 196217248135
genetic analysis of six primary monosomes and one tertiary monosome in gossypium hirsutum. 196317248191
the genetics of flowering response in cotton. v. fruiting behavior of gossypium hirsutum and gossypium barbadense in interspecific hybrids. 196517248253
tests for the association of marker loci with chromosomes in gossypium hirsutum by the use of aneuploids. 196517248254
the anomalous behavior of the ghost spot of gossypium anomalum in amphidiploid gossypium hirsutum. 196517248255
the inheritance of gossypol level in gossypium i. additive, dominance, epistatic, and maternal effects associated with seed gossypol in two varieties of gossypium hirsutum l. 196817248408
genetical studies concerning the distribution of trichomes on the leaves of gossypium hirsutum l. 196817248422
alternate-1 and alternate-2 disjunctions in heterozygous reciprocal translocations.alternate-1 and alternate-2 orientation of chromosomes, as well as the two types of adjacent orientation, were observed cytologically in the ring configurations of three reciprocal translocation heterozygotes of gossypium hirsutum l. the observations indicate that the two types of alternate orientation should be characteristic of ring-forming translocations.197417248653
genetic lengths and break points in twelve chromosomes of gossypium hirsutum involved in ten reciprocal translocations.chromosome configurations were recorded in about 5500 pollen mother cells (pmc's) in 2n and 2n-1 (missing the intact a-genome chromosome) heterozygotes of ten reciprocal translocations involving six a-genome chromosomes (h1, h2, h3, h4, h6 and h7) and six d-genome chromosomes (h14, h15, h16, h19, h20 and h21) of gossypium hirsutum. from these records, chiasma frequencies at each of six positions were determined for nine translocations and at two positions for one. these frequencies were used to ...197817248811
rapid development of enhanced atrazine degradation in a dundee silt loam soil under continuous corn and in rotation with cotton.mississippi delta cotton (gossypium hirsutum l.) production in rotation with corn (zea mays l.) was evaluated in field experiments from 2000 to 2005 at stoneville, mississippi. plots maintained under minimum tillage were established in 2000 on a dundee silt loam with treatments including continuous cotton or corn and alternate cotton-corn rotations. mineralization and dissipation of 14c [ring]-labeled atrazine were evaluated in the laboratory on soils collected prior to herbicide application in ...200717263485
transgenic cotton over-producing spinach sucrose phosphate synthase showed enhanced leaf sucrose synthesis and improved fiber quality under controlled environmental conditions.prior data indicated that enhanced availability of sucrose, a major product of photosynthesis in source leaves and the carbon source for secondary wall cellulose synthesis in fiber sinks, might improve fiber quality under abiotic stress conditions. to test this hypothesis, a family of transgenic cotton plants (gossypium hirsutum cv. coker 312 elite) was produced that over-expressed spinach sucrose-phosphate synthase (sps) because of its role in regulation of sucrose synthesis in photosynthetic a ...200717287885
expression of cp4 epsps in microspores and tapetum cells of cotton (gossypium hirsutum) is critical for male reproductive development in response to late-stage glyphosate applications.plants expressing agrobacterium sp. strain cp4 5-enolpyruvylshikimate-3-phosphate synthase (cp4 epsps) are known to be resistant to glyphosate, a potent herbicide that inhibits the activity of the endogenous plant epsps. the rr1445 transgenic cotton line (current commercial line for roundup ready cotton) was generated using the figwort mosaic virus (fmv) 35s promoter to drive the expression of the cp4 epsps gene, and has excellent vegetative tolerance to glyphosate. however, with high glyphosate ...200617309724
characterization and expression of a putative retinoblastoma protein binding gene from gossypium hirsutum.a genomic clone representing a putative retinoblastoma binding (rbb) protein was isolated from a gossypium hirsutum bac library. alignment of the gene sequence with the cdna sequence indicated the gene consists of six exons that have standard eukaryotic splice junctions. the conceptual spliced transcript was 98% identical to tc37171 in the tigr gene index, however it encoded an orf 107 amino acids longer than best deduced protein from tc37171. the conceptual translation of the genomic clone was ...200617312951
a quantitative method for the determination of cyclopropenoid fatty acids in cottonseed, cottonseed meal, and cottonseed oil (gossypium hirsutum) by high-performance liquid chromatography.cyclopropenoid fatty acids (cpfas), found in cottonseed, have been shown to have detrimental health effects to susceptible livestock. previous quantitative analytical methods for the determination of cpfas expressed these acids in terms of their relative abundance with respect to other fatty acids in the oil, necessitating the concurrent analysis of other fatty acids. the proposed analytical method describes the quantitation of three relevant cpfas for cotton (malvalic acid, sterculic acid, and ...200717323967
cloning and characterization of cotton ghbg gene encoding beta-glucosidase.beta-1,4-glucosidase (bg, ec3.2.1.21), one of three cellulases, is a widespread family of enzymes involved in the metabolism of cell wall polysaccharides in both prokaryocytes and eukaryotes. here, we report the isolation of a full-length cdna encoding beta-1,4-glucosidase protein (designated as ghbg) and its putative function in the process of fiber development and in yeast. through random sequencing of the cotton fiber cdna library from 7235 germplasm line, with elite fiber quality in gossypiu ...200617343209
detection and identification of rhamnogalacturonan lyase activity in intercellular spaces of expanding cotton cotyledons.rhamnogalacturonan lyase (rg lyase) activity has been detected and its relative activity measured in vivo during the expansion of cotton (gossypium hirsutum l.) cotyledons. rhamnogalacturonan (rg) oligomers labeled with a fluorescent tag were injected into the intercellular spaces of cotton cotyledons and, after incubation, the digested substrate was rinsed out. enzyme digestion products were detected and identified by capillary zone electrophoresis. rhamnogalacturonan lyase products were identi ...200717346264
common subtropical and tropical nonpollen food sources of the boll weevil (coleoptera: curculionidae).it is known that substantial boll weevil, anthonomus grandis grandis boheman, individuals can survive mild subtropical winters in some habitats, such as citrus orchards. our study shows that endocarp of the fruit from prickly pear cactus, opuntia engelmannii salm-dyck ex. engel.; orange, citrus sinensis l. osbeck.; and grapefruit, citrus paradisi macfad., can sustain newly emerged adult boll weevils for >5 mo, which is the duration of the cotton-free season in the subtropical lower rio grande va ...200717349122
molecular cloning and expression characteristics of alternative oxidase gene of cotton (gossypium hirsutum).a novel alternative oxidase (aox) gene derived from cotton (gossypium hirsutum), designated as ghaox1, was cloned with race-pcr. the full-length cdna of ghaox1was 1,298 bp in size, containing a 996 bp open reading frame (orf) which corresponds to a precursor protein of 332 amino acid residues with a calculated molecular mass of 37.5 kda. the predicted amino acid sequence exhibited 68.4%, 68.1%, 59.4%, and 69.8% homology to the alternative oxidases of arabidopsis thaliana, nicotiana tabacum, sola ...200817351819
effects of crop rotation, tillage, and fertilizer applications on sorghum head insects.rotations, tillage, and fertilizer treatments can affect yield, costs, and profitability in sorghum, sorghum bicolor (l.) moench, depending on their effects on pests. rotation or planting different crops reduces soil erosion and pests that build up when a field is planted to the same crop each year. minimum tillage reduces the number of trips over a field, lessening soil compaction and reducing costs. we examined the effects of fertilizer, tillage, and rotation with cotton, gossypium hirsutum l. ...200717370814
cross-resistance responses of crylac-selected heliothis virescens (lepidoptera: noctuidae) to the bacillus thuringiensis protein vip3a.one susceptible and three cry1ac-resistant strains of tobacco budworm, heliothis virescens (f.) (lepidoptera: noctuidae), were used in laboratory studies to determine the level of cross-resistance between the bacillus thuringiensis (berliner) toxins cry1ac and vip3a by using concentration-mortality and leaf tissue experiments. concentration-mortality data demonstrated that the three cry1ac-resistant h. virescens strains, yhd2, kcbhyb, and cxc, were at least 215- to 316-fold resistant to cry1ac c ...200717370826
a polymerase chain reaction screen of field populations of heliothis virescens for a retrotransposon insertion conferring resistance to bacillus thuringiensis toxin.the evolution of pest resistance to transgenic crop plants producing insecticidal toxins from bacillus thuringiensis (bt) berliner poses a continuing threat to their sustainable use in agriculture. one component of the u.s.-wide resistance management plan for bt cotton, gossypium hirsutum l., involves monitoring the frequency of resistance alleles in field populations. however, existing methods are expensive and may not detect recessive resistance alleles until their frequencies are too high for ...200717370827
simultaneous growth and emission measurements demonstrate an interactive control of methanol release by leaf expansion and stomata.emission from plants is a major source of atmospheric methanol. growing tissues contribute most to plant-generated methanol in the atmosphere, but there is still controversy over biological and physico-chemical controls of methanol emission. methanol as a water-soluble compound is thought to be strongly controlled by gas-phase diffusion (stomatal conductance), but growth rate can follow a different diurnal rhythm from that of stomatal conductance, and the extent to which the emission control is ...200717374874
detection by rapd-pcr of polymorphism in populations of bemisia tabaci (genn.) collected on four host plants from iran.the sweetpotato whitefly, bernisia tabaci (genn.) (hom: aleyrodidae) is a major pest of field crops, vegetables and ornamentals in iran. in this study, the infested leaves of cucumber (cucurnis sativus l.) zucchini (cucurbita pepo l.) eggplant (solanum melongena l.) and cotton (gossypium hirsutum l.) with whitefly nymphs and pupae were collected from iran, and were transferred to the laboratory. the newly emerged males and females of each population were released separately into a large cage set ...200617385530
comparative study of the five biological parameters of cotton whitefly bemisia tabaci and silverleaf whitefly b. argentifolii bellows and perring reared on cotton under laboratory condition.the five biological parameters of sweetpotato whitefly, bemisia tabaci (genn.) and silverleaf whitefly bemisia argentifolii bellows and perring (hom: aleyrodidae) as an important pest of cotton were compared on cotton in laboratory condition. the infested leaves containing nymphs and pupae were collected from cotton fields in iran. experiments were conducted in a growth chamber under 24+/-2 degrees c, 55+/-3% rh and 16:8 (l:d) photoperiod on cotton, gossypium hirsutum l. the newly emerged popula ...200617385531
changes in homogalacturonans and enzymes degrading them during cotton cotyledon expansion.changes in homogalacturonans (hgs) and enzymes degrading them have been investigated during cotton (gossypium hirsutum l.) cotyledon expansion. using an in vivo assay for pectin-degrading enzymes that involves fluorescent labeled oligomers of gala as substrate and capillary electrophoresis for product analysis, we found that endo- and exo-polygalacturonases are present in the cotyledon extracellular spaces, and there are dramatic changes in the levels of both activities as the cotyledons change ...200717395221
the role of induced mutation in conversion of photoperiod dependence in cotton.wild cotton germplasm resources are largely underutilized because of photoperiod-dependent flowering of "exotic" cottons. the objectives of this work were to explore the genome-wide effect of induced mutation in photoperiod-converted induced cotton mutants, estimating the genetic change between mutant and wild-type cottons using simple sequence repeats (ssrs) as well as understand the pattern of ssr mutation in induced mutagenesis. three groups of photoperiod-converted radiomutants ((32)p) inclu ...200717406024
gene action and morphological characteristics of pink flower and pink filament mutants in cotton (gossypium hirsutum l.). 200617406101
computational identification of micrornas and their targets in gossypium hirsutum expressed sequence tags.micrornas (mirnas) are a class of non-coding rnas that regulate gene post-transcriptional expression in animals and plants. comparatively genomic computational methods have been developed to predict new mirnas in worms, humans, and arabidopsis. here we present an est (expressed sequence tags)--and gss (genomic survey sequences)-based combined approach for the detection of novel mirnas in gossypium hirsutum. this was initiated by using previously known mirna sequences from arabidopsis, rice and o ...200717408884
integrative mapping of gossypium hirsutum l. by meiotic fluorescent in situ hybridization of a tandemly repetitive sequence (b77).we determined the relative positions of the tandem-repeat molecular cytogenetic marker b77, translocation breakpoints, and telosome arms in gossypium hirsutum cytogenetic stocks by fluorescence in situ hybridization (fish) analysis of meiotic quadrivalents in 16 single and 2 double translocation heterozygotes and five monotelodisomics. results delimited the b77 fish locus to the right arm of the d-subgenome chromosome 14 (14r) and the short arm (14sh), respectively. by equating 14r with 14sh and ...200717409065
microcolinearity and genome evolution in the adha region of diploid and polyploid cotton (gossypium).genome sizes vary by several orders of magnitude, driven by mechanisms such as illegitimate recombination and transposable element proliferation. prior analysis of the cesa region in two cotton genomes that diverged 5-10 million years ago (ma), and acquired a twofold difference in genome size, revealed extensive local conservation of genic and intergenic regions, with no evidence of the global genome size difference. the present study extends the comparison to include bac sequences surrounding t ...200717461788
qtl mapping of yield and yield components for elite hybrid derived-rils in upland cotton.a population of 180 recombinant inbred lines (rils) was developed by single seed descended from the cross of high yield upland cotton (gossypium hirsutum l.) varieties zhongmiansuo12 (zms12) and 8891, the two parents of xiangzamian2 (xzm2). a genetic linkage map consisting of 132 loci and covering 865.20 cm was constructed using the ril population chiefly with ssr markers. yield and yield components were investigated for rils in three environments in china. the purpose of the present research wa ...200717469776
molecular characterization of cotton 14-3-3l gene preferentially expressed during fiber elongation.the 14-3-3 protein, highly conserved in all eukaryotic cells, is an important regulatory protein. it plays an important role in the growth, amplification, apoptosis, signal transduction, and other crucial life activities of cells. a cdna encoding a putative 14-3-3 protein was isolated from cotton fiber cdna library. the cdna, designated as gh14-3-3l (gossypium hirsutum 14-3-3-like), is 1,029 bp in length (including a 762 bp long open reading frame and 5'-/3'-untranslated regions) and deduced a p ...200717469787
nitrogen and phosphorus runoff losses from variable and constant intensity rainfall simulations on loamy sand under conventional and strip tillage systems.further studies on the quality of runoff from tillage and cropping systems in the southeastern usa are needed to refine current risk assessment tools for nutrient contamination. our objective was to quantify and compare effects of constant (ic) and variable (iv) rainfall intensity patterns on inorganic nitrogen (n) and phosphorus (p) losses from a tifton loamy sand (plinthic kandiudult) cropped to cotton (gossypium hirsutum l.) and managed under conventional (ct) or strip-till (st) systems. we s ...201317485716
molecular characterization of four adf genes differentially expressed in cotton.actin depolymerizing factor (adf), highly conserved in all eukaryotic cells, is a low molecular mass of actin-binding protein, which plays a key role in modulating the polymerizing and depolymerizing of the actin filaments. four cdnas (designated ghadf2, ghadf3, ghadf4, and ghadf5, respectively) encoding adf proteins were isolated from cotton (gossypium hirsutum) fiber cdna library. ghadf2 cdna is 705 bp in length and deduces a protein with 139 amino acids. ghadf3 cdna is 819 bp in length and en ...200717498633
analysis of gene expression in cotton fiber initials.cotton (gossypium hirsutum l.) fibers are trichomes that initiate from the ovule epidermis. little is known about the developmental pathway causing fiber to differentiate from ovular epidermal cells even though limits on the number of cells that differentiate into fiber will limit yield.200717506877
quantitative analysis of cotton (gossypium hirsutum) lint trash by fluorescence spectroscopy.the presence of cotton plant botanical components, or trash, embedded in lint subsequent to harvesting and ginning is an important criterion in the classification of baled cotton by the u.s. department of agriculture agricultural marketing service. the trash particles may be reduced in size to the point that specific trash types are not identifiable by image or gravimetric analysis, and it is desirable to quantify different trash types so that processing lines may be optimized for removal of the ...200717536818
molecular cloning and characterization of crmdr1, a novel mdr-type abc transporter gene from catharanthus roseus.a novel gene encoding a mdr-like abc transporter protein was cloned from catharanthus roseus, a medicinal plant with more than 120 kinds of secondary metabolites, through rapid amplification of cdna ends (race). this gene (named as crmdr1; genbank accession no.: dq660356) had a total length of 4395 bp with an open reading frame of 3801 bp, and encoded a predicted polypeptide of 1266 amino acids with a molecular weight of 137.1 kda. the crmdr1 protein shared 59.8, 62.5, 60.0 and 58.2% identity wi ...200717541839
[the inheritance of an ultra-dwarf plant mutant from upland cotton].the inheritance of an ultra-dwarf plant mutant from upland cotton (gossypium hirsutum l.) was studied, which showed that the mutant was controlled by single recessive quality gene. this gene was denominated as du tentatively. no similar mutant has been found in upland cotton. the mutation could not normally flower and produce bolls under natural conditions, and its mature height was only 10.5 cm. when treated with exogenous ga3, it could normally flower and boll, and plant height could reach 57. ...200717548311
transgene integration and organization in cotton (gossypium hirsutum l.) genome.while genetically modified upland cotton (gossypium hirsutum l.) varieties are ranked among the most successful genetically modified organisms (gmo), there is little knowledge on transgene integration in the cotton genome, partly because of the difficulty in obtaining large numbers of transgenic plants. in this study, we analyzed 139 independently derived t0 transgenic cotton plants transformed by agrobacterium tumefaciens strain agl1 carrying a binary plasmid ppzp-gfp. it was found by pcr that ...200817549600
[estimation of the efficiency of seed irradiation by thermal neutrons for inducing chromosomal aberrations in m1 of cotton gossypium hirsutum l].exposure of cotton seeds to thermal neutrons at doses of 15, 25, and 35 gy was shown to induce many altered plants, including sterile and chimeric ones. most of these phenotypic changes were shown to result from novel genomic, chromosomal, and desynaptic mutations. the presence of these mutations in the karyotype of m, plants often decreased meiotic index and pollen fertility. in translocation forms, the decrease in pollen fertility was caused by the prevalence of quadrivalents in form of rings ...200717555126
cloning and expression of the chloroplast copper/zinc-superoxide dismutase gene in upland cotton (gossypium hirsutum l.).a full-length 1,043-base-pair cdna clone encoding a chloroplast copper/zinc superoxide dismutase (cu/zn-sod) of upland cotton was first isolated by rapid amplification of cdna ends (race) from the leaves of the cotton (gossypium hirsutum l.) variety 'cri36'. nucleotide sequence analysis of the clone revealed that it contained the complete coding sequence of the mature sod isozyme subunit, along with a 60-amino acid transit peptide at n-terminal. the amino acid sequence predicted from the full-le ...200717556806
a novel map kinase gene in cotton (gossypium hirsutum l.), ghmapk, is involved in response to diverse environmental stresses.the mitogen-activated protein kinase (mapk) cascade is one of the major and evolutionally conserved signaling pathways and plays pivotal role in the regulation of stress and developmental signals in plants. here, a novel gene, termed gossypium hirsutum mapk (ghmapk), was isolated from cotton. the full-length cdna of ghmapk encodes for a 372 amino acid protein that contains all 11 of the mapk conserved subdomains and the phosphorylation-activation motif, tey. amino acid sequence alignment reveale ...200717562283
ghdet2, a steroid 5alpha-reductase, plays an important role in cotton fiber cell initiation and elongation.cotton (gossypium hirsutum l.) fibers, one of the most important natural raw materials for textile industry, are highly elongated trichomes from epidermal cells of cotton ovules. det2, an arabidopsis steroid 5d-reductase, is considered to catalyze a major rate-limiting in brassinosteroid (br) biosynthesis. to understand the role of brs in cotton fiber development, ghdet2, which putatively encodes a steroid 5alpha-reductase by sequence comparison, was cloned from developing fiber cells. in vitro ...200717565582
investigation of some yield and fibre quality characteristics of interspecific hybrid (gossypium hirsutum l. x g. barbadense l.) cotton varieties.interspecific hybrid cottons (gossypium hirsutum l. x g. barbadense l.) have great both yield and quality potential. this study was conducted to determine potential yields and quality characteristics of hybrid cotton varieties in southeastern anatolia region of turkey. the experiment was set out a completely randomized block design with four replications during 2003 and 2004 at university of dicle, faculty of agriculture experimental field. seven interspecific hybrid cotton varieties (48-08, sev ...200717567438
differentiating tobacco budworm and corn earworm using near-infrared spectroscopy.near-infrared spectroscopy (nirs) was used to develop a simple and quick technique to differentiate two economically important species, the tobacco budworm, heliothis cirescens (f.), and corn earworm, helicoverpa zea (boddie), which are major pests of cotton, gossypium hirsutum l., in the southern united states. in practice, it is difficult to distinguish the two species during their immature stages using morphological characteristics unless expensive microscopy equipment or trained technicians ...200717598536
resistance to bacillus thuringiensis toxin cry2ab in a strain of helicoverpa armigera (lepidoptera: noctuidae) in australia.transgenic cotton, gossypium hirsutum l., expressing the crylac and cry2ab genes from bacillus thuringiensis (bt) berliner variety kurstaki in a pyramid (bollgard ii) was widely planted for the first time in australia during the 2004-2005 growing season. before the first commercial bollgard ii crops, limited amounts of cotton expressing only the crylac gene (ingard) was grown for seven seasons. no field failures due to resistance to crylac toxin were observed during that period and a monitoring ...200717598553
mating compatibility among helicoverpa armigera (lepidoptera: noctuidae) occurring on selected host plants and bt cotton survivors.helicoverpa armigera (hiibner) (lepidoptera: noctuidae) is a well-known polyphagous insect pest. mating compatibility among the insects occurring on different host plants is essential for free gene flow among populations. we tested the extent of crossability and fecundity of the insects that survived on bacillus thuringiensis (bt) cotton with those occurring on pigeon pea, cajanus cajun (l.) millsp., non-bt cotton, gossypium hirsutum l.; sunflower, helianthus annuus; sorghum, sorghum bicolor l. ...200717598554
cross-resistance studies of cry1ac-resistant strains of helicoverpa armigera (lepidoptera: noctuidae) to cry2ab.bioassays and binding tests between cry toxins (crylaa, crylab, crylac, and cry2ab) and brush-border membrane vesicles (bbmvs) from larvae of a bacillus thurningiensis (bt)-susceptible (96s) and two crylac-resistant strains (btr and lfr10) were conducted for investigating cross-resistance of crylac-resistant strains to cry2ab in helicoverpa armigera (hiibner) (lepidoptera: noctuidae). the resistance ratio (rr) values of the btr and lfr10 strains to crylac and cry2ab were 2,971- and 1.1-fold and ...200717598555
cloning of a mads box gene (ghmads3) from cotton and analysis of its homeotic role in transgenic tobacco.a mads box gene (ghmads3) was cloned from cotton (gossypium hirsutum l.) based on est sequences. the predicted protein sequence of ghmads3 showed 85%, 73%, and 62% identity with theobroma cacao tcag, antirrhinum majus far, and arabidopsis thaliana ag, respectively, and was grouped with ag homologues when the full length sequences excluding n-extensions were compared. ghmads3 expressed in the wild type cotton flower primarily in stamens and carpels, which was comparable to ag in arabidopsis. howe ...200717601612
analysis of differentially expressed genes in genic male sterility cotton (gossypium hirsutum l.) using cdna-aflp.cdna amplified fragment length polymorphism (cdna-aflp) analysis was used to investigate the differentially expressed genes between sterile and fertile plants of ms5ms6 double-recessive genic male sterility (gms) two-type line cotton (gossypium hirsutum l.) at different stages, i.e., sporogenous cell stage, pollen mother cell (pmc) stage, and pollen grain stage. seventeen differentially expressed fragments were identified. functional analysis indicated that their corresponding genes may particip ...200717601613
analysis of genetic effects of nuclear-cytoplasmic interaction on quantitative traits: genetic model for diploid plants.a genetic model was proposed for simultaneously analyzing genetic effects of nuclear, cytoplasm, and nuclear-cytoplasmic interaction (nci) as well as their genotype by environment (ge) interaction for quantitative traits of diploid plants. in the model, the nci effects were further partitioned into additive and dominance nuclear-cytoplasmic interaction components. mixed linear model approaches were used for statistical analysis. on the basis of diallel cross designs, monte carlo simulations show ...200717601616
the 9-lipoxygenase ghlox1 gene is associated with the hypersensitive reaction of cotton gossypium hirsutum to xanthomonas campestris pv malvacearum.hypersensitive reaction (hr) cell death of cotton to the incompatible race 18 from xanthomonas campestris pathovar malvacearum (xcm) is associated with 9s-lipoxygenase activity (lox) responsible for lipid peroxidation. here, we report the cloning of cotton (gossypium hirsutum l.) lox gene (ghlox1) and the sequencing of its promoter. ghlox1 was found to be highly expressed during xcm induced hr. sequence analysis showed that ghlox1 is a putative 9-lox, and ghlox1 promoter contains sa and ja respo ...200717611116
development of one set of chromosome-specific microsatellite-containing bacs and their physical mapping in gossypium hirsutum l.fluorescence in situ hybridization (fish), using bacterial artificial chromosome (bac) clone as probe, is a reliable cytological technique for chromosome identification. it has been used in many plants, especially in those containing numerous small chromosomes. we previously developed eight chromosome-specific bac clones from tetraploid cotton, which were used as excellent cytological markers for chromosomes identification. here, we isolated the other chromosome-specific bac clones to make a com ...200717632699
a cotton ascorbate peroxidase is involved in hydrogen peroxide homeostasis during fibre cell development.reactive oxygen species (ros) play important roles in multiple physiological processes such as cellular signalling and stress responses, whereas, the hydrogen peroxide (h(2)o(2)) scavenging enzyme ascorbate peroxidase (apx) participates in the regulation of intracellular ros levels. here, a cotton (gossypium hirsutum) cytosolic apx1 (ghapx1) was identified to be highly accumulated during cotton fibre elongation by proteomic analysis. ghapx1 cdna contained an open reading frame of 753-bp encoding ...200717635221
laser capture microdissection and cdna microarrays used to generate gene expression profiles of the rapidly expanding fibre initial cells on the surface of cotton ovules.cotton (gossypium hirsutum l.) fibre initial cells undergo a rapid cellular re-programming around anthesis to form the long cellulose fibres prized for textile manufacture. on the day of anthesis the cotton fibre initial cells balloon out from the ovule surface and so are clearly distinguished from adjacent epidermal pavement cells. to enhance our understanding of the molecular processes that determine which cells become fibres and why adjacent epidermal cells remain in a different developmental ...200717636323
transmission of cotton seed and boll rotting bacteria by the southern green stink bug (nezara viridula l.).to determine the ability of the southern green stink bug (sgsb) (nezara viridula l.) to transmit pantoea agglomerans into cotton (gossypium hirsutum) bolls.200717650204
meiosis in a triploid hybrid of gossypium: high frequency of secondary bipolar spindles at metaphase ii.studies on meiosis in pollen mother cells (pmcs) of a triploid interspecific hybrid (3x = 39 chromosomes, aad) between tetraploid gossypium hirsutum (4n = 2x = 52,aadd) and diploid g. arboreum (2n = 2x = 26,aa) are reported. during meiotic metaphase i, 13 aa bivalents and 13 d univalents are expected in the hybrid. however, only 28% of the pmcs had this expected configuration. the rest of the pmcs had between 8 and 12 bivalents and between 12 and 17 univalents. univalents lagged at anaphase i, a ...200717656848
comparative characteristics and gene action in three petal-spotted mutants of gossypium hirsutum. 200717656854
host plants of the tarnished plant bug (heteroptera: miridae) in central texas.the tarnished plant bug, lygus lineolaris (palisot de beauvois), has taken on added importance as a pest of cotton in the cotton belt after successful eradication efforts for the boll weevil (anthonomus grandis grandis boheman). because the southern blacklands region of central texas is in advanced stages of boll weevil eradication, blooming weeds and selected row crops were sampled during a 3-yr study to determine lygus species composition and associated temporal host plants. l. lineolaris was ...200717716463
determination of the pore size of cell walls of living plant cells.the limiting diameter of pores in the walls of living plant cells through which molecules can freely pass has been determined by a solute exclusion technique to be 35 to 38 angstroms for hair cells of raphanus sativus roots and fibers of gossypium hirsutum, 38 to 40 angstroms for cultured cells of acer pseudoplatanus, and 45 to 52 angstroms for isolated palisade parenchyma cells of the leaves of xanthium strumarium and commelina communis. these results indicate that molecules with diameters larg ...197917735052
the induction of microsomal nadph:cytochrome p450 and nadh:cytochrome b(5) reductases by long-term salt treatment of cotton (gossypium hirsutum l.) and bean (phaseolus vulgaris l.) plants.we studied the effect of salinity on the activity of microsomal nadph:cytochrome p450 reductase (cpr, ec 1.6.2.4) and nadh:ferricytochrome b(5) oxidoreductase (b5r, ec 1.6.2.2) in two dicotyledonous plant species differing in their sensitivity to salt, cotton (gossypium hirsutum l. cv ogosta) and common bean (phaseolus vulgaris l. cv dobrujanski 7). a significant inhibition of fresh weight of salt-treated bean plants was observed, while cotton was affected to a much lesser degree. nacl applicati ...200717761430
variable rainfall intensity and tillage effects on runoff, sediment, and carbon losses from a loamy sand under simulated rainfall.the low-carbon, intensively cropped coastal plain soils of georgia are susceptible to runoff, soil loss, and drought. reduced tillage systems offer the best management tool for sustained row crop production. understanding runoff, sediment, and chemical losses from conventional and reduced tillage systems is expected to improve if the effect of a variable rainfall intensity storm was quantified. our objective was to quantify and compare effects of a constant (ic) intensity pattern and a more real ...200717766829
antiquity of american polyploid cotton.fragments of a boll of gossypium hirsutum l. from archeological excavations near tehuacán, mexico, prove that this species existed in 5800 b. c. no doubt remains that american tetraploid cotton species originated through natural hiybridization.196417772618
cotton ghdreb1 increases plant tolerance to low temperature and is negatively regulated by gibberellic acid.the transcription factors c-repeat binding factors/dehydration-responsive element binding proteins (cbfs/drebs) control the expression of many stress-inducible genes in arabidopsis. a cdna clone, designated ghdreb1, was isolated from cotton (gossypium hirsutum) by cdna library screening. northern blot analysis indicated that mrna accumulation of ghdreb1 was induced by low temperatures and salt stress, but was not induced by abscisic acid (aba) or drought stress in cotton seedlings. transgenic to ...200717803642
transgenic bacillus thuringiensis (bt) cotton (gossypium hirsutum) allomone response to cotton aphid, aphis gossypii, in a closed-dynamics co(2) chamber (cdcc).allocation of allomones of transgenic bacillus thuringiensis gossypium hirsutum (bt cotton) (cv. gk-12) and non-bt-transgenic cotton (cv. simian-3) grown in elevated co(2) in response to infestation by cotton aphid, aphis gossypii glover, was studied in a closed-dynamics co(2) chamber. significant increases in foliar condensed tannin and carbon/nitrogen ratio for gk-12 and simian-3 were observed in elevated co(2) relative to ambient co(2,) as partially supported by the carbon nutrient balance hy ...200717805479
a strategy for designing multi-taxa specific reference gene systems. example of application--ppi phosphofructokinase (ppi-ppf) used for the detection and quantification of three taxa: maize (zea mays), cotton (gossypium hirsutum) and rice (oryza sativa).in the first part of the paper, we report the description of a new strategy for the development of a plant reference gene system that can be used for genetically modified organism (gmo) analysis. on the basis of in silico research for candidate genes, the design of degenerate primers allowed the obtention of genomic sequences of the selected gene ppi-phosphofructokinase ( ppi-ppf) for nine taxa in which gmos have been developed. the comparison and the analysis of inter- and intraspecies sequence ...200717824661
expression partitioning between genes duplicated by polyploidy under abiotic stress and during organ development.allopolyploidy has been a prominent mode of speciation and a recurrent process during plant evolution and has contributed greatly to the large number of duplicated genes in plant genomes [1-4]. polyploidy often leads to changes in genome organization and gene expression [5-9]. the expression of genes that are duplicated by polyploidy (termed homeologs) can be partitioned between the duplicates so that one copy is expressed and functions only in some organs and the other copy is expressed only in ...200717825563
a majority of cotton genes are expressed in single-celled fiber.multicellular eukaryotes contain a diversity of cell types, presumably differing from one another in the suite of genes expressed during development. at present, little is known about the proportion of the genome transcribed in most cell types, nor the degree to which global patterns of expression change during cellular differentiation. to address these questions in a model plant system, we studied the unique and highly exaggerated single-celled, epidermal seed trichomes ("cotton") of cultivated ...200817849148
toxicity of seven foliar insecticides to four insect parasitoids attacking citrus and cotton pests.laboratory studies were carried out to compare the toxicity of seven foliar insecticides to four species of adult beneficial insects representing two families of hymenoptera: aphelinidae (aphytis melinus debach, eretmocerus eremicus rose & zolnerowich, and encarsiaformosa gahan) and mymaridae (gonatocerus ashmeadi girault) that attack california red scale, aonidiella aurantii (maskell); sweetpotato whitefly, bemisia tabaci (gennadius) (both e. eremicus and e. formosa); and glassy-winged sharpsho ...200717849851
effects of proportion and configuration of bacillus thuringiensis cotton on pest abundance, damage, and yield.bacillus thuringiensis (bt) transgenic cotton, gossypium hirsutum l., kills several economically important pests, reducing injury and increasing yields. refuges of non-bt cotton are currently planted with bt cotton in different designs to slow pest resistance evolution. to compare the effects of differences in bt/non-bt plant heterogeneity found in different refuge designs on square (flower bud) damage, abscissions, sap-feeding herbivore densities, and yield in cotton, four types of 24-row cotto ...200717849898
sequence analysis and characterization of vacuolar-type h+ -atpase proteolipid transcript from acanthus ebracteatus vah1.the vacuolar-type h+ -atpase (v-atpase) is a multimeric enzyme with diverse functions in plants such as nutrient transport, flowering, stress tolerance, guard cell movement and development. a partial sequence of v-atpase proteolipid was identified among the expressed sequence tags (ests) generated from acanthus ebracteatus, and selected for full-length sequencing. the 876-nucleotide cdna consists of an open reading frame of 165 amino acids. the deduced amino acid sequence displays high similarit ...200817852357
[disturbed structure and function of chloroplasts during blocked biosynthesis of 5'-aminolevulinic acid in the light].xantha-702 mutant of cotton (gossypium hirsutum l.) proved to have blocked synthesis of 5'-aminolevulinic acid in the light. accordingly, mutant leaves accumulated 2-5% chlorophyll of baseline. mutant plants demonstrated disturbed production of pigment-protein complexes of photosystems i (psi) and ii (psii) and generation of the chloroplast membrane system blocked at the early stages, largely, at the stages of vesicles and single short thylakoid. the functional activity of the psi and psii react ...201617853692
a proteinaceous elicitor sm1 from the beneficial fungus trichoderma virens is required for induced systemic resistance in maize.we have previously shown that the beneficial filamentous fungus trichoderma virens secretes the highly effective hydrophobin-like elicitor sm1 that induces systemic disease resistance in the dicot cotton (gossypium hirsutum). in this study we tested whether colonization of roots by t. virens can induce systemic protection against a foliar pathogen in the monocot maize (zea mays), and we further demonstrated the importance of sm1 during maize-fungal interactions using a functional genomics approa ...200717885089
molecular markers linked to the rf2 fertility restorer gene in cotton.cytoplasmic male sterility (cms) is a maternally inherited trait in which plants do not produce viable pollen. fertility in plants with cms can be recovered by nuclear restorer genes. most restorer genes cloned so far are members of the pentatricopeptide repeat (ppr) protein family. the objective of our study was to use the cms-d8 and restoration (rf2) system of cotton (gossypium hirsutum l.) to develop more dna markers for the rf2 gene. in a backcross population with 112 plants, segregation of ...200717893722
establishment of azotobacter on plant roots: chemotactic response, development and analysis of root exudates of cotton (gossypium hirsutum l.) and wheat (triticum aestivum l.).biofertilizers contribute in n(2) fixation, p solubilization, phytohormone production and thus enhance plant growth. beneficial plant-microbe interactions and the stability and effectiveness of biofertilizer depend upon the establishment of bacterial strains in the rhizosphere of the plant. this interaction depends upon many factors, one of them being plant exudates. root exudates are composed of small organic molecules like carbonic acids, amino acids or sugars etc., which are released into the ...200717910096
cotton ghkch2, a plant-specific kinesin, is low-affinitive and nucleotide-independent as binding to microtubule.kinesin is an atp-driven microtubule motor protein that plays important roles in control of microtubule dynamics, intracellular transport, cell division and signal transduction. the kinesin superfamily is composed of numerous members that are classified into 14 subfamilies. animal kinesins have been well characterized. in contrast, plant kinesins have not yet to be characterized adequately. here, a novel plant-specific kinesin gene, ghkch2, has been cloned from cotton (gossypium hirsutum) fibers ...200717927906
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