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reproduction of meloidogyne marylandi and m. incognita on several poaceae.the susceptibility of 22 plant species to meloidogyne marylandi and m. incognita was examined in three greenhouse experiments. inoculum of m. marylandi was eggs from cultures maintained on zoysia matrella "cavalier" or cynodon dactylon x c. trasvaalensis "tifdwarf". inoculum of m. incognita was eggs from cultures maintained on solanum lycopersicum 'rutgers'. in each host test the inoculum density was 2,000 nematode eggs/pot. none of the three dicot species tested (gossypium hirsutum, arachis hyp ...200922661770
cotton (gossypium hirsutum l.) boll rotting bacteria vectored by the brown stink bug, euschistus servus (say) (hemiptera: pentatomidae).to determine the capacity of the brown stink bug (bsb) (euschistus servus) to transmit an infective pantoea agglomerans strain sc 1-r into cotton (gossypium hirsutum) bolls.201627261673
relationship between piercing-sucking insect control and internal lint and seed rot in southeastern cotton (gossypium hirsutum l.).in 1999, crop consultants scouting for stink bugs (hemiptera spp.) in south carolina discovered a formerly unobserved seed rot of cotton that caused yield losses ranging from 10 to 15% in certain fields. the disease has subsequently been reported in fields throughout the southeastern cotton belt. externally, diseased bolls appeared undamaged; internally, green fruit contain pink to dark brown, damp, deformed lint, and necrotic seeds. in greenhouse experiments, we demonstrated transmission of the ...201526470293
the gossypium hirsutum wrky gene ghwrky39-1 promotes pathogen infection defense responses and mediates salt stress tolerance in transgenic nicotiana benthamiana.our results indicate that overexpression of the ghwrky39 - 1 gene enhances resistance to pathogen infection and tolerance to high salt and oxidative stress in transgenic nicotiana benthamiana.201424337818
overexpression of cotton ghmkk4 enhances disease susceptibility and affects abscisic acid, gibberellin and hydrogen peroxide signalling in transgenic nicotiana benthamiana.mitogen-activated protein kinase (mapk) cascades are involved in plant development, stress responses and hormonal signal transduction. mapk kinases (mapkks), as the key nodes in these cascades, link mapks and mapkk kinases (mapkkks). in this study, ghmkk4, a novel group c mapkk gene from cotton (gossypium hirsutum), was isolated and identified. its expression can be induced by various stresses and signalling molecules. the overexpression of ghmkk4 in nicotiana benthamiana enhanced its susceptibi ...201423980654
cotton ghmpk6a negatively regulates osmotic tolerance and bacterial infection in transgenic nicotiana benthamiana, and plays a pivotal role in development.mitogen-activated protein kinase (mapk) cascades play important roles in the perception of external signals and the generation of suitable responses. cotton (gossypium hirsutum) is an important fibre-producing and oil-producing crop worldwide. however, few mapks and their interaction partners have been functionally characterized in cotton. in the present study, the group a mapk g. hirsutum (gh)mpk6a was identified and characterized. ghmpk6a expression can be induced through multiple defence-rela ...201323957843
characterization of rhizobacterial strain rs-2 with acc deaminase activity and its performance in promoting cotton growth under salinity stress.a plant growth-promoting rhizobacterial strain rs-2 with 1-aminocyclopropane-1-carboxylate (acc) deaminase activity was isolated from salinized soils using acc as the sole nitrogen source. based on its physiological and biochemical properties and 16s rdna sequence analysis, this strain was identified as raoultella planticola. the maximum value of nitrogen fixation, dissolved phosphorus and dissolved potassium of rs-2 were 148.8 μg/ml, 205.0 and 4.31 mg/l, respectively within 192 h liquid culture ...201222806112
gossypium arboreum accessions resistant to rotylenchulus reniformis.in the southeastern united states, reniform nematode (rotylenchulus reniformis) is a serious pest of upland cotton (gossypium hirsutum), a species which has no naturally occurring resistance against this nematode. to identify sources of reniform nematode resistance in species closely related to upland cotton, 222 g. arboreum accessions from the u.s. germplasm collection were evaluated in repeated growth chamber experiments. in initial screenings, root infection was measured 4 wks after inoculati ...201628154428
postinfection development of rotylenchulus reniformis on resistant gossypium barbadense accessions.the reniform nematode (rotylenchulus reniformis) causes significant cotton (gossypium hirsutum) losses in the southeastern united states. the research objective was to describe the effects of two resistant g. barbadense lines (cultivar tx 110 and accession gb 713) on development and fecundity of reniform nematode. nematode development and fecundity were evaluated on the resistant lines and susceptible g. hirsutum cultivar deltapine 16 in three repeated growth chamber experiments. nematode develo ...201526941458
transcriptome analysis of cotton (gossypium hirsutum l.) genotypes that are susceptible, resistant, and hypersensitive to reniform nematode (rotylenchulus reniformis).reniform nematode is a semi-endoparasitic nematode species causing significant yield loss in numerous crops, including cotton (gossypium hirsutum l.). an rna-sequencing analysis was conducted to measure transcript abundance in reniform nematode susceptible (dp90 & sg747), resistant (barbren-713), and hypersensitive (lonren-1) genotypes of cotton (gossypium hirsutum l.) with and without reniform nematode infestation. over 90 million trimmed high quality reads were assembled into 84,711 and 80, 35 ...201526571375
the effect of soil texture and irrigation on rotylenchulus reniformis and cotton.the reniform nematode, rotylenchulus reniformis, is the most damaging nematode pathogen of cotton in alabama. soil texture is currently being explored as a basis for the development of economic thresholds and management zones within a field. trials to determine the reproductive potential of r. reniformis as influenced by soil type were conducted in microplot and greenhouse settings during 2008 to 2010. population density of r. reniformis was significantly influenced by soil texture and exhibited ...201323833324
impact of rotylenchulus reniformis on cotton yield as affected by soil texture and irrigation.the effects of soil type, irrigation, and population density of rotylenchulus reniformis on cotton were evaluated in a two-year microplot experiment. six soil types, fuquay sand, norfolk sandy loam, portsmouth loamy sand, muck, cecil sandy loam, and cecil sandy clay, were arranged in randomized complete blocks with five replications. each block had numerous plots previously inoculated with r. reniformis and two or more noninoculated microplots per soil type, one half of which were irrigated in e ...201022736865
natural migration of rotylenchulus reniformis in a no-till cotton system.rotylenchulus reniformis is the most damaging nematode pathogen of cotton in alabama. it is easily introduced into cotton fields via contaminated equipment and, when present, is difficult and costly to control. a trial to monitor the natural migration of r. reniformis from an initial point of origin was established in 2007 and studied over two growing seasons in both irrigated and non-irrigated no-till cotton production systems. vermiform females, juveniles and males reached a horizontal distanc ...201022736863
molecular and morphological characterization and biological control capabilities of a pasteuria ssp. parasitizing rotylenchulus reniformis, the reniform nematode.rotylenchulus reniformis is one of 10 described species of reniform nematodes and is considered the most economically significant pest within the genus, parasitizing a variety of important agricultural crops. rotylenchulus reniformis collected from cotton fields in the southeastern us were observed to have the nematode parasitic bacterium pasteuria attached to their cuticles. challenge with a pasteuria-specific monoclonal antibody in live immuno-fluorescent assay (ifa) confirmed the discovery of ...201022736858
classification of rotylenchulus reniformis numbers in cotton using remotely sensed hyperspectral data on self-organizing maps.rotylenchulus reniformis is one of the major nematode pests capable of reducing cotton yields by more than 60%, causing estimated losses that may exceed millions of dollars u.s. therefore, early detection of nematode numbers is necessary to reduce these losses. this study investigates the feasibility of using remotely sensed hyperspectral data (reflectances) of cotton plants affected with different nematode population numbers with self-organizing maps (som) in correlating and classifying nematod ...201022736855
saccharibacillus endophyticus sp. nov., an endophyte of cotton.a gram-positive-staining, facultatively aerobic, endospore-forming bacterial strain, isolated from the stem tissue of cotton (gossypium hirsutum) was subjected to a detailed taxonomic study using a polyphasic approach. based on 16s rrna gene sequence similarity comparisons, strain jm-1350t grouped into the genus saccharibacillus, and was most closely related to the type strain of saccharibacillus sacchari (96.5 % 16s rrna gene sequence similarity), followed by the type strains of saccharibacillu ...201627613514
overexpression of cotton pyl genes in arabidopsis enhances the transgenic plant tolerance to drought stress.pyr/pyl/rcar proteins are putative abscisic acid (aba) receptors that play important roles in plant responses to biotic and abiotic stresses. in this study, 27 predicted pyl proteins were identified in cotton (gossypium hirsutum). sequence analysis showed they are conserved in structures. phylogenetic analysis showed that cotton pyl family could be categorized into three groups. yeast two-hybrid assay revealed that the ghpyl proteins selectively interacted with some ghpp2c proteins. quantitative ...201728388505
the glutathione peroxidase gene family in gossypium hirsutum: genome-wide identification, classification, gene expression and functional analysis.the plant glutathione peroxidase (gpx) family consists of multiple isoenzymes with distinct subcellular locations, tissue-specific expression patterns and environmental stress responses. in this study, 13 putative gpxs from the genome of gossypium hirsutum (ghgpxs) were identified and a conserved pattern among plant gpxs were exhibited, besides this they also responded to multiple environmental stresses and we predicted that they had hormone responsive cis-elements in their promoter regions. mos ...201728300195
ghmyb1 regulates scw stage-specific expression of the ghgdsl promoter in the fibres of gossypium hirsutum l.secondary cell wall (scw) biosynthesis is an important stage of the cotton fibre development, and its transcriptional regulation is poorly understood. we selected the gossypium hirsutum gdsl (ghgdsl) lipase/hydrolase gene (cotad_74480), which is expressed during scw biosynthesis (19 through to 25 days postanthesis; dpa), for study. t1 -transgenic cotton lines expressing the β-glucuronidase (gus) reporter under the control of a 1026-bp promoter fragment of ghgdsl (pghgdsl ) showed 19 dpa stage-sp ...201728182326
functional divergence of ghcfe5 homoeologs revealed in cotton fiber and arabidopsis root cell development.in ghcfe5 homoeologs, ghcfe5d interacted with more actin homologs and stronger interaction activity than ghcfe5a. ghcfe5d - but not ghcfe5a -overexpression severely disrupted actin cytoskeleton organization and significantly suppressed cell elongation. homoeologous genes are common in polyploid plants; however, their functional divergence is poorly elucidated. allotetraploid upland cotton (gossypium hirsutum, aadd) is the most widely cultivated cotton; accounting for more than 90 % of the world' ...201626759310
functional characterization of ghsoc1 and ghmads42 homologs from upland cotton (gossypium hirsutum l.).in arabidopsis flowering pathway, mads-box genes encode transcription factors, with their structures and functions highly conserved in many species. in our study, two mads-box genes ghsoc1 and ghmads42 (gossypium hirsutum l.) were cloned from upland cotton ccri36 and transformed into arabidopsis. ghsoc1 was additionally transformed into upland cotton. comparative analysis demonstrated sequence conservation between ghsoc1 and ghmads42 and genes of other plant species. tissue-specific expression a ...201626566835
genome-scale analysis of the cotton kcs gene family revealed a binary mode of action for gibberellin a regulated fiber growth.production of β-ketoacyl-coa, which is catalyzed by 3-ketoacyl-coa synthase (kcs), is the first step in very long chain fatty acid (vlcfa) biosynthesis. here we identified 58 kcs genes from gossypium hirsutum, 31 from g. arboreum and 33 from g. raimondii by searching the assembled cotton genomes. the gene family was divided into the plant-specific fae1-type and the more general elo-type. kcs transcripts were widely expressed and 32 of them showed distinct subgenome-specific expressions in one or ...201626399709
a novel nap member ghnap is involved in leaf senescence in gossypium hirsutum.premature leaf senescence has a negative influence on the yield and quality of cotton, and several genes have been found to regulate leaf senescence. howeer, many underlying transcription factors are yet to be identified. in this study, a nap-like transcription factor (ghnap) was isolated from gossypium hirsutum. ghnap has the typical nac structure and a conserved novel subdomain in its divergent transcription activation region (tar). ghnap was demonstrated to be a nuclear protein, and it showed ...201525991739
analyzing serial cdna libraries revealed reactive oxygen species and gibberellins signaling pathways in the salt response of upland cotton (gossypium hirsutum l.).by comparing series full-length cdna libraries stressed and control, the dynamic process of salt stress response in upland cotton was studied, and reactive oxygen species and gibberellins signaling pathways were proposed. the upland cotton is the most important fiber plant with highly salt tolerance. however, the molecular mechanism underlying salt tolerance in domesticated cotton was unclear. here, seven full-length cdna libraries were constructed for seedling roots of upland cotton 'zhong g 5' ...201525700980
steam explosion distinctively enhances biomass enzymatic saccharification of cotton stalks by largely reducing cellulose polymerization degree in g. barbadense and g. hirsutum.in this study, steam explosion pretreatment was performed in cotton stalks, leading to 5-6 folds enhancements on biomass enzymatic saccharification distinctive in gossypium barbadense and gossypium hirsutum species. sequential 1% h2so4 pretreatment could further increase biomass digestibility of the steam-exploded stalks, and also cause the highest sugar-ethanol conversion rates probably by releasing less inhibitor to yeast fermentation. by comparison, extremely high concentration alkali (16% na ...201525656866
quantitative proteomics and transcriptomics reveal key metabolic processes associated with cotton fiber initiation.an itraq-based proteomics of ovules from the upland cotton species gossypium hirsutum and its fuzzless-lintless mutant was performed, and finally 2729 proteins that preferentially accumulated at anthesis in wild-type ovules were identified. we confirmed that the gene expression levels of 2005 among these proteins also increased by performing an rna sequencing transcriptomics. expression of proteins involved in carboxylic acid metabolism, small-molecule metabolic processes, hormone regulation, an ...201525449837
overexpression of a cotton gene that encodes a putative transcription factor of ap2/erebp family in arabidopsis affects growth and development of transgenic plants.in the study, a gene encoding a putative ethylene response factor of ap2/erebp family was isolated from cotton (gossypium hirsutum) and designated as gherf12. sequence alignment showed that gherf12 protein contains a central ap2/erf domain (58 amino acids) with two functional conserved amino acid residues (ala14 and asp19). transactivation assay indicated that gherf12 displayed strong transcription activation activity in yeast cells, suggesting that this protein may be a transcriptional activato ...201324194949
ghcax3 gene, a novel ca(2+)/h(+) exchanger from cotton, confers regulation of cold response and aba induced signal transduction.as a second messenger, ca(2+) plays a major role in cold induced transduction via stimulus-specific increases in [ca(2+)]cyt, which is called calcium signature. during this process, caxs (ca(2+)/h(+) exchangers) play critical role. for the first time, a putative ca(2+)/h(+) exchanger ghcax3 gene from upland cotton (gossypium hirsutum cv. 'yz-1') was isolated and characterized. it was highly expressed in all tissues of cotton except roots and fibers. this gene may act as a regulator in cotton's r ...201323776653
cotton plasma membrane intrinsic protein 2s (pip2s) selectively interact to regulate their water channel activities and are required for fibre development.aquaporins are thought to be associated with water transport and play important roles in cotton (gossypium hirsutum) fibre elongation. among aquaporins, plasma membrane intrinsic proteins (pips) constitute a plasma-membrane-specific subfamily and are further subdivided into pip1 and pip2 groups. in this study, four fibre-preferential ghpip2 genes were functionally characterized. the selective interactions among ghpip2s and their interaction proteins were studied in detail to elucidate the molecu ...201323656428
a cotton gene encoding myb-like transcription factor is specifically expressed in pollen and is involved in regulation of late anther/pollen development.in flowering plants, pollen development is a highly programmed process, in which a lot of genes are involved. in this study, a gene, designated as ghmyb24, encoding r2r3-myb-like protein was isolated from cotton. ghmyb24 protein is localized in the cell nucleus and acts as a transcriptional activator. northern blot analysis revealed that ghmyb24 transcripts were predominantly detected in anthers. it was further found that strong expression of ghmyb24 was mainly detected in pollen and was regulat ...201323447105
a cotton gene encoding a plasma membrane aquaporin is involved in seedling development and in response to drought stress.cotton (gossypium hirsutum), the most important textile crop worldwide, often encounters abiotic stress such as drought and waterlog during its growth season (summer), and its productivity is significantly limited by adverse factors. to investigate the molecular adaptation mechanisms of this plant species to abiotic stress, a gene encoding the plasma membrane intrinsic protein (pip) was isolated in cotton, and designated as ghpip2;7. quantitative reverse transcriptase polymerase chain reaction a ...201323178915
ghagp31, a cotton non-classical arabinogalactan protein, is involved in response to cold stress during early seedling development.arabinogalactan proteins (agps), a superfamily of highly glycosylated hydroxyproline-rich glycoproteins, are widely implicated in plant growth and development. a gene (including its cdna), designated ghagp31, encoding a non-classical agp protein was isolated from cotton (gossypium hirsutum). the deduced ghagp31 protein contains the conserved features of non-classical agps: a putative signal peptide, n-terminal histidine-rich stretch, middle repetitive proline-rich domain and a cysteine-containin ...201222222112
virus-induced gene silencing in cultivated cotton (gossypium spp.) using tobacco rattle virus.the study described here has optimized the conditions for virus-induced gene silencing (vigs) in three cultivated cotton species (gossypium hirsutum, g. arboreum, and g. herbaceum) using a tobacco rattle virus (trv) vector. the system was used to silence the homolog of the arabidopsis thaliana chloroplastos alterados 1 (atcla1) gene, involved in chloroplast development, in g. herbaceum, g. arboreum, and six commercial g. hirsutum cultivars. all plants inoculated with the trv vector to silence cl ...201626603442
ghpsy, a phytoene synthase gene, is related to the red plant phenotype in upland cotton (gossypium hirsutum l.).carotenoids are important accessory pigments in plants that are essential for photosynthesis. phytoene synthase (psy), a rate-controlling enzyme in the carotenoid biosynthesis pathway, has been widely characterized in rice, maize, and sorghum, but at present there are no reports describing this enzyme in cotton. in this study, ghpsy was identified as a candidate gene for the red plant phenotype via a combined strategy using: (1) molecular marker data for loci closely linked to r1; (2) the whole- ...201424718783
development of agrobacterium-mediated virus-induced gene silencing and performance evaluation of four marker genes in gossypium barbadense.gossypiumbarbadense is a cultivated cotton species and possesses many desirable traits, including high fiber quality and resistance to pathogens, especially verticilliumdahliae (a devastating pathogen of gossypium hirsutum, the main cultivated species). these elite traits are difficult to be introduced into g. hirsutum through classical breeding methods. in addition, genetic transformation of g. barbadense has not been successfully performed. it is therefore important to develop methods for eval ...201324023833
variovorax gossypii sp. nov., isolated from gossypium hirsutum.a beige-pigmented bacterial strain (jm-310t), isolated from the healthy internal root tissue of 4-week-old cotton (gossypium hirsutum, cultivar 'des-119') in tallassee (macon county), alabama, usa, was studied taxonomically. the isolate produced small rod-shaped cells, which showed a gram-negative staining behaviour. a comparison of the 16s rrna gene sequence of the isolate revealed 99.2, 98.8, 98.7, 98.7, 98.1 and 97.6 % similarity to the 16s rrna gene sequences of the type strains of variovora ...201526341669
pyramiding b genes in cotton achieves broader but not always higher resistance to bacterial blight.near-isogenic lines of upland cotton (gossypium hirsutum) carrying single, race-specific genes b4, bin, and b7 for resistance to bacterial blight were used to develop a pyramid of lines with all possible combinations of two and three genes to learn whether the pyramid could achieve broad and high resistance approaching that of l. a. brinkerhoff's exceptional line im216. isogenic strains of xanthomonas axonopodis pv. malvacearum carrying single avirulence (avr) genes were used to identify plants ...201424655289
quantitative analysis of resistance in cotton to three new isolates of the bacterial blight pathogen.genetic variability for virulence of the bacterial blight pathogen [xanthomonas campestris pv malvacearum (smith) dye] on cotton (gossypium hirsutum l.) has been shown by the identification of 19 races of the pathogen based on disease reactions of a set of ten host differentials. this study was conducted to determine the inheritance of host resistance to three recently identified isolates of x. campestris pv malvacearum, which are virulent on the entire set of differentials. true leaves of tamco ...199024226445
gherf-iib3 regulates the accumulation of jasmonate and leads to enhanced cotton resistance to blight disease.the phytohormone jasmonic acid (ja) and its derivatives, collectively referred to as jasmonates, regulate many developmental processes, but are also involved in the response to numerous abiotic/biotic stresses. thus far, powerful reverse genetic strategies employing perception, signalling or biosynthesis mutants have broadly contributed to our understanding of the role of ja in the plant stress response and development, as has the chemical gain-of-function approach based on exogenous application ...201627291786
genetic transformation of cotton with a harpin-encoding gene hpaxoo confers an enhanced defense response against verticillium dahliae kleb.the soil-borne fungal pathogen verticillium dahliae kleb causes verticillium wilt in a wide range of crops including cotton (gossypium hirsutum). to date, most upland cotton varieties are susceptible to v. dahliae and the breeding for cotton varieties with the resistance to verticillium wilt has not been successful. hpa1xoo is a harpin protein from xanthomonas oryzae pv. oryzae which induces the hypersensitive cell death in plants. when hpa1xoo was transformed into the susceptible cotton line z3 ...201323143497
the verticillium-specific protein vdscp7 localizes to the plant nucleus and modulates immunity to fungal infections.fungal pathogens secrete effector proteins to suppress plant basal defense for successful colonization. resistant plants, however, can recognize effectors by cognate r proteins to induce effector-triggered immunity (eti). by analyzing secretomes of the vascular fungal pathogen verticillium dahliae, we identified a novel secreted protein vdscp7 that targets the plant nucleus. the green fluorescent protein (gfp)-tagged vdscp7 gene with either a mutated nuclear localization signal motif or with add ...201728407259
development and survival of spodoptera exigua (lepidoptera: noctuidae) on alternate crops in cotton cropping pattern, with implications to integrated pest management.spodoptera exigua (hübner) is a polyphagous pest that shifts its population to different hosts during its life cycle to receive nutritive advantages. therefore, demographic evaluation of alternate hosts is important for effective pest management. here, we have evaluated castor (ricinus communis l.), cauliflower (brassica oleracea l.), cotton (gossypium hirsutum l.), okra (abelmoschus esculentus l.), and spinach (spinacia oleracea l.) for growth, survival, and population development of s. exigua. ...201728383691
molecular typing of mealybug phenacoccus solenopsis populations from different hosts and locations in punjab, india.true identity and existence of genetic variability in mealy bug from different regions holds immense significance for adopting appropriate control measures along with predicting the development of any biotypes. mealy bug, phenacoccus solenopsis adults were collected from four host plants i.e., gossypium hirsutum--cotton (c), abelmoschus esculentus--okra (o), pennisetum glaucum--napier bajra (b) and a weed--parthenium hysterophorus (p) in five cotton growing districts i.e., abohar, bathinda, mans ...201223029900
synthesis and evaluation of heterocyclic analogues of bromoxynil.one attractive strategy to discover more active and/or crop-selective herbicides is to make structural changes to currently registered compounds. this strategy is especially appealing for those compounds with limited herbicide resistance and whose chemistry is accompanied with transgenic tools to enable herbicide tolerance in crop plants. bromoxynil is a photosystem ii (psii) inhibitor registered for control of broadleaf weeds in several agronomic and specialty crops. recently at the university ...201424354444
how do leaf hydraulics limit stomatal conductance at high water vapour pressure deficits?a reduction in leaf stomatal conductance (g) with increasing leaf-to-air difference in water vapour pressure (d) is nearly ubiquitous. ecological comparisons of sensitivity have led to the hypothesis that the reduction in g with increasing d serves to maintain leaf water potentials above those that would cause loss of hydraulic conductance. a reduction in leaf water potential is commonly hypothesized to cause stomatal closure at high d. the importance of these particular hydraulic factors was te ...200616898024
uptake and metabolism of clomazone in tolerant-soybean and susceptible-cotton photomixotrophic cell suspension cultures.studies were conducted to determine the uptake and metabolism of the pigment synthesis inhibiting herbicide clomazone in tolerant-soybean (glycine max [l.] merr. cv corsoy) and susceptible-cotton (gossypium hirsutum [l.] cv stoneville 825) photomixotrophic cell suspensions. soybean and cotton on a whole plant level are tolerant and susceptible to clomazone, respectively. preliminary studies indicated that i(50) values for growth, chlorophyll (chl), beta-carotene, and lutein were, respectively, > ...199016667349
effects of irradiance on relative growth rates, net assimilation rates, and leaf area partitioning in cotton and three associated weeds.cotton (gossypium hirsutum l. var. ;stoneville 213'), velvetleaf (abutilon theophrasti medic.), redroot pigweed (amaranthus retroflexus l.), and hemp sesbania (sesbania exaltata [raf.] cory) were grown in a controlled environment room at 31/25 c day/night temperature and three irradiances: 90, 320, and 750 mueinsteins meter(-2) second(-1). from total dry weights and leaf areas determined at intervals during the first exponential phase of growth, we used mathematical growth analysis techniques to ...197816660454
effects of irradiance during growth on adaptive photosynthetic characteristics of velvetleaf and cotton.we grew velvetleaf (abutilon theophrasti medic.) and cotton (gossypium hirsutum l. var. stoneville 213) at three irradiances and determined the photosynthetic responses of single leaves to a range of six irradiances from 90 to 2000 mueinsteins m(-2)sec(-1). in air containing 21% o(2), velvetleaf and cotton grown at 750 mueinsteins m(-2)sec(-1) had maximum photosynthetic rates of 18.4 and 21.9 mg of co(2) dm(-2)hr(-1), respectively. maximum rates for leaves grown at 320 and 90 mueinsteins m(-2)se ...197816660302
host-associated differentiation in a highly polyphagous, sexually reproducing insect herbivore.insect herbivores may undergo genetic divergence on their host plants through host-associated differentiation (had). much of what we know about had involves insect species with narrow host ranges (i.e., specialists) that spend part or all their life cycle inside their hosts, and/or reproduce asexually (e.g., parthenogenetic insects), all of which are thought to facilitate had. however, sexually reproducing polyphagous insects can also exhibit had. few sexually reproducing insects have been teste ...201526257868
caffeic acid and glycerol are constituents of the suberin layers in green cotton fibres.the fibres of the green-lint mutant (lg) of cotton (gossypium hirsutum l.) are suberized and contain a large proportion of wax. the unidentified components of the wax were separated into a colourless fluorescent fraction and a yellow pigmented fraction. using ultraviolet spectroscopy and nuclear-magneticresonance ((1)h-nmr) spectroscopy, esterified trans-caffeic acid was identified as the only phenolic component in the colourless fraction. this fraction was further purified and was shown to cont ...199324178505
interference between redroot pigweed (amaranthus retroflexus l.) and cotton (gossypium hirsutum l.): growth analysis.redroot pigweed is one of the injurious agricultural weeds on a worldwide basis. understanding of its interference impact in crop field will provide useful information for weed control programs. the effects of redroot pigweed on cotton at densities of 0, 0.125, 0.25, 0.5, 1, 2, 4, and 8 plants m(-1) of row were evaluated in field experiments conducted in 2013 and 2014 at institute of cotton research, caas in china. redroot pigweed remained taller and thicker than cotton and heavily shaded cotton ...201526057386
physiological and antioxidant responses of cotton and spurred anoda under interference and mild drought.the influence of plant interference and a mild drought on gas exchange and oxidative stress was investigated using potted plants of two cotton species (gossypium hirsutum l. cv. delta pine 5415, and gossypium barbadense l. cv. pima s-7) and spurred anoda (anoda cristata l. schlecht.) of the malvaceae. without interference, cotton and spurred anoda had similar net photosynthesis (pnet) but different pigment profiles. stomatal conductance (gs) and transpiration rate (e) were greater in spurred ano ...200314504299
studies on the sites expressing evolutionary changes in the structure of eukaryotic 5s ribosomal rna.we have determined the complete sequences of 5s rrnas from a lamprey (lampetra reissneri), a lancelet (branchiostoma belcheri), silkworms (philosamia cynthia ricini, bombyx mori, antheraea pernyi), and a silkworm hybrid (artificially fertilized hybrid species of philosamia cynthia ricini male x bombyx mori female), as well as those of cotton seeds (gossypium hirsutum l.). having compared more than 170 eukaryotic 5s rrnas of which seven sequences have been determined by our group as mentioned abo ...19883146644
cloning and functional analysis of a novel ascorbate peroxidase (apx) gene from anthurium andraeanum.an 888-bp full-length ascorbate peroxidase (apx) complementary dna (cdna) gene was cloned from anthurium andraeanum, and designated as anapx. it contains a 110-bp 5'-noncoding region, a 28-bp 3'-noncoding region, and a 750-bp open reading frame (orf). this protein is hydrophilic with an aliphatic index of 81.64 and its structure consisting of α-helixes, β-turns, and random coils. the anapx protein showed 93%, 87%, 87%, 87%, and 86% similarities to the apx homologs from zantedeschia aethiopica, v ...201324302711
identification of a new cotton disease caused by an atypical cotton leafroll dwarf virus in argentina.an outbreak of a new disease occurred in cotton (gossypium hirsutum) fields in northwest argentina starting in the 2009-10 growing season and is still spreading steadily. the characteristic symptoms of the disease included slight leaf rolling and a bushy phenotype in the upper part of the plant. in this study, we determined the complete nucleotide sequences of two independent virus genomes isolated from cotton blue disease (cbd)-resistant and -susceptible cotton varieties. this virus genome comp ...201728035870
effects of single and dual species herbivory on the behavioural responses of three thrips species to cotton seedlings.this study investigated the olfactory responses of three thrips species [frankliniella schultzei trybom, f. occidentalis pergrande and thrips tabaci lindeman (thysanoptera: thripidae)] to cotton seedlings [gossypium hirsutum l. (malvales: malvaceae)] simultaneously damaged by different combinations of herbivores. cotton seedlings were damaged by foliar feeding tetranychus urticae koch (trombidiforms: tetranychidae), helicoverpa armigera hübner (lepidoptera: noctuidae), aphis gossypii glover (hem ...201627029603
interspecific associations between cycloneda sanguinea and two aphid species (aphis gossypii and hyadaphis foeniculi) in sole-crop and fennel-cotton intercropping systems.aphids cause significant damage to crop plants. studies regarding predator-prey relationships in fennel (foeniculum vulgare mill.) and cotton (gossypium hirsutum l.) crops are important for understanding essential ecological interactions in the context of intercropping and for establishing pest management programs for aphids. this study evaluated the association among hyadaphis foeniculi (passerini) (hemiptera: aphididae), aphis gossypii glover (hemiptera: aphididae) and cycloneda sanguinea (l.) ...201526241862
how does the host-specialized aphid deal with food deficiency?aphis gossypii glover shows obvious host specialization, with cucurbit- and cotton-specialized biotypes or host races in many regions. because its annual natal host crops senesce earlier the cucurbit-specialized biotype may suffer food deficiency. the method this biotype uses to overcome this challenge is still poorly understood. in order to understand the potential of the cucurbit-specialized biotype aphids in host shift and usage, the performance of this biotype on cotton (gossypium hirsutum), ...201424526656
within-plant distribution of cotton aphid (hemiptera: aphididae) in cotton cultivars with colored fibers.we describe the vertical and horizontal distribution of the cotton aphid aphis gossypii glover within a cotton plant in two cotton (gossypium hirsutum linnaeus) cultivars (brs safira and brs rubí) with colored fiber over the time. measurements of aphid population dynamics and distribution in the cotton plants were recorded in intervals of seven days. the number of apterous or alate aphids and their specific locations were recorded, using as a reference point the location of nodes on the mainstem ...201222782536
aphid antixenosis in cotton is activated by the natural plant defence elicitor cis-jasmone.upon insect herbivory, plants can release blends of volatile organic compounds (vocs) that modify herbivore and natural enemy behaviour. we have shown recently that cotton, gossypium hirsutum, emits a blend of defence vocs that repels the cotton aphid, aphis gossypii, upon herbivory by this notorious crop pest, including (z)-3-hexenyl acetate, (e)-4,8-dimethyl-1,3,7-nonatriene (dmnt), methyl salicylate and (e,e)-4,8,12-trimethyl-1,3,7,11-tridecatetraene (tmtt). in this study, we investigated cha ...201222516741
effect of reproductive mode on host plant utilization of melon aphid (hemiptera: aphididae).variation in the reproductive mode of melon aphid aphis gossypii glover occurred on the large geographic scale, but the performance of different reproductive modes to use host plant is poorly understood. life tables of melon aphid population that undergo the anholocyclic, androcyclic, and intermediate reproductive mode were conducted on different host plants. the results showed that the anholocyclic and androcyclic strains could become adults and produce offspring on cotton gossypium hirsutum l. ...201022182553
identification of semiochemicals released by cotton, gossypium hirsutum, upon infestation by the cotton aphid, aphis gossypii.the cotton aphid, aphis gossypii (homoptera: aphididae), is increasing in importance as a pest worldwide since the introduction of bt-cotton, which controls lepidopteran but not homopteran pests. the chemical ecology of interactions between cotton, gossypium hirsutum (malvaceae), a. gossypii, and the predatory lacewing chrysoperla lucasina (neuroptera: chrysopidae), was investigated with a view to providing new pest management strategies. behavioral tests using a four-arm (pettersson) olfactomet ...201121671083
remotely sensing arthropod and nutrient stressed plants: a case study with nitrogen and cotton aphid (hemiptera: aphididae).remote sensing can be used in combination with ground sampling to detect aphid- (aphis gossypii glover) infested cotton (gossypium hirsutum l.). changes in wavelengths in the near-infrared (nir) have proven useful for such detection, but these changes can be confused with other factors stressing plants, such as water deficiency and nutrient status. this study was designed to test the utility of this technology to distinguish between two factors stressing plants: nitrogen deficiency and aphids. f ...201022127176
interactive effects of elevated co2 and cotton cultivar on tri-trophic interaction of gossypium hirsutum, aphis gossyppii, and propylaea japonica.information on the effects of enriched co2 on both the chemical composition of plants and the consequences of such changes for performance of a herbivore and its predator is an important first step in understanding the responses of plants and insects to global environmental change. we examined interactions across three trophic levels, cotton, gossypium hirsutum, an aphid herbivore, aphis gossypii glover, and a coccinellid predator, propylaea japonica (thunberg), as affected by elevated co2 conce ...200818348793
resistance of aphis gossypii (homoptera: aphididae) to fenvalerate and imidacloprid and activities of detoxification enzymes on cotton and cucumber.resistance of two strains of cotton aphid, aphis gossypii glover, to fenvalerate and imidacloprid were determined on cotton (gossypium hirsutum l.) and cucumber (cucumis sativa l.) after resistance selection of one strain to fenvalerate for 16 consecutive generations, and of a second strain to imidacloprid for 12 consecutive generations on cotton in greenhouses. dose-response and activities of detoxication enzymes of the fenvalerate-resistant strain (r-fenvalerate), the imidacloprid-resistant st ...200212020021
fatty acid and proximate composition of bee bread.palynological spectrum, proximate and fatty acid (fa) composition of eight bee bread samples of different botanical origins were examined and significant variations were observed. the samples were all identified as monofloral, namely castanea sativa (94.4%), trifolium spp. (85.6%), gossypium hirsutum (66.2%), citrus spp. (61.4%) and helianthus annuus (45.4%). each had moisture content between 11.4 and 15.9%, ash between 1.9 and 2.54%, fat between 5.9 and 11.5%, and protein between 14.8 and 24.3% ...201628115909
functional characterization of agamous-subfamily members from cotton during reproductive development and in response to plant hormones.expression analysis of the ag -subfamily members from g. hirsutum during flower and fruit development. reproductive development in cotton, including the fruit and fiber formation, is a complex process; it involves the coordinated action of gene expression regulators, and it is highly influenced by plant hormones. several studies have reported the identification and expression of the transcription factor family mads-box members in cotton ovules and fibers; however, their roles are still elusive d ...201728176007
genome-wide identification and functional analysis of the tify gene family in response to drought in cotton.jasmonates control many aspects of plant biological processes. they are important for regulating plant responses to various biotic and abiotic stresses, including drought, which is one of the most serious threats to sustainable agricultural production. however, little is known regarding how jasmonate zim-domain (jaz) proteins mediate jasmonic acid signals to improve stress tolerance in cotton. this represents the first comprehensive comparative study of tify transcription factors in both diploid ...201627640194
insights into interspecific hybridization events in allotetraploid cotton formation from characterization of a gene-regulating leaf shape.the morphology of cotton leaves varies considerably. phenotypes, including okra, sea-island, super-okra, and broad leaf, are controlled by a multiple allele locus, l2 okra leaf (l2°) is an incomplete mutation that alters leaf shape by increasing the length of lobes with deeper sinuses. using a map-based cloning strategy, we cloned the l2 locus gene, which encodes a late meristem identity 1 (lmi1)-like transcription factor (ghokra). silencing ghokra leads to a change in phenotype from okra to bro ...201627558139
functional characterization of a basic helix-loop-helix (bhlh) transcription factor ghdel65 from cotton (gossypium hirsutum).cotton fiber is proposed to share some similarity with the arabidopsis thaliana leaf trichome, which is regulated by the myb-bhlh-wd40 transcription complex. although several myb transcription factors and wd40 family proteins in cotton have been characterized, little is known about the role of bhlh family proteins in cotton. here, we report that ghdel65, a bhlh protein from cotton (gossypium hirsutum), is a functional homologue of arabidopsis glabra3 (gl3) and enhancer of glabra3 (egl3) in regul ...201627080593
promoter isolation and characterization of ghao-like1, a gossypium hirsutum gene similar to multicopper oxidases that is highly expressed in reproductive organs.cotton is one of the most economically important cultivated crops. it is the major source of natural fiber for the textile industry and an important target for genetic modification for both biotic stress and herbicide tolerance. therefore, the characterization of genes and regulatory regions that might be useful for genetic transformation is indispensable. the isolation and characterization of new regulatory regions is of great importance to drive transgene expression in genetically modified cro ...201626692462
molecular cloning and expression analysis of five ghraxs in upland cotton (gossypium hirsutum l.).the formation of axillary meristems in leaf axils is a prerequisite for the development of lateral shoots, which largely contribute to plant architecture. several transcription factor-encoding genes, including cuc3, rax, las, lof1, and rox, have been cloned by screening for axillary meristem mutants in arabidopsis thaliana. these genes will facilitate our understanding of the mechanisms underlying axillary meristem development. in this study, we report the cloning of five genes from cotton (goss ...201526505359
kinesin-4 functions in vesicular transport on cortical microtubules and regulates cell wall mechanics during cell elongation in plants.in plants, anisotropic cell expansion depends on cortical microtubules that serve as tracks along which macromolecules and vesicles are transported by the motor kinesins of unknown identities. we used cotton (gossypium hirsutum) fibers that underwent robust elongation to discover kinesins that are involved in cell elongation and found gh kinesin-4a expressed abundantly. the motor was detected by immunofluorescence on vesicle-like structures that were associated with cortical microtubules. in ara ...201525600279
temperature responses of mesophyll conductance differ greatly between species.the temperature responses of mesophyll conductance (gm ) were investigated for nine species using carbon isotope techniques combining tunable diode laser spectroscopy and gas exchange measurements. species included the evergreen trees eucalyptus pauciflora and quercus engelmannii; the tropical evergreen tree lophostemon confertus; as well as the herbaceous species nicotiana tabacum, oryza sativa, triticum aestivum, gossypium hirsutum, glycine max and arabidopsis thaliana. responses varied from a ...201525224884
upland cotton gene ghfpf1 confers promotion of flowering time and shade-avoidance responses in arabidopsis thaliana.extensive studies on floral transition in model species have revealed a network of regulatory interactions between proteins that transduce and integrate developmental and environmental signals to promote or inhibit the transition to flowering. previous studies indicated flowering promoting factor 1 (fpf1) gene was involved in the promotion of flowering, but the molecular mechanism was still unclear. here, fpf1 homologous sequences were screened from diploid gossypium raimondii l. (d-genome, n =  ...201424626476
atwuschel promotes formation of the embryogenic callus in gossypium hirsutum.upland cotton (gossypium hirsutum) is one of the most recalcitrant species for in vitro plant regeneration through somatic embryogenesis. callus from only a few cultivars can produce embryogenic callus (ec), but the mechanism is not well elucidated. here we screened a cultivar, cri24, with high efficiency of ec produce. the expression of genes relevant to ec production was analyzed between the materials easy to or difficult to produce ec. quantitative pcr showed that cri24, which had a 100% ec d ...201424498119
sugar and auxin signaling pathways respond to high-temperature stress during anther development as revealed by transcript profiling analysis in cotton.male reproduction in flowering plants is highly sensitive to high temperature (ht). to investigate molecular mechanisms of the response of cotton (gossypium hirsutum) anthers to ht, a relatively complete comparative transcriptome analysis was performed during anther development of cotton lines 84021 and h05 under normal temperature and ht conditions. in total, 4,599 differentially expressed genes were screened; the differentially expressed genes were mainly related to epigenetic modifications, c ...201424481135
association between photosynthesis and contrasting features of minor veins in leaves of summer annuals loading phloem via symplastic versus apoplastic routes.foliar vascular anatomy and photosynthesis were evaluated for a number of summer annual species that either load sugars into the phloem via a symplastic route (cucumis sativus l. cv. straight eight; cucurbita pepo l. cv. italian zucchini romanesco; citrullus lanatus l. cv. faerie hybrid; cucurbita pepo l. cv. autumn gold) or an apoplastic route (nicotiana tabacum l.; solanum lycopersicum l. cv. brandywine; gossypium hirsutum l.; helianthus annuus l. cv. soraya), as well as winter annual apoplast ...201424450755
a study on the influence of different promoter and 5'utr (urm) cassettes from arabidopsis thaliana on the expression level of the reporter gene β glucuronidase in tobacco and cotton.several reports of promoters from plants, viral and artificial origin that confer high constitutive expression are known. among these the camv 35s promoter is used extensively for transgene expression in plants. we identified candidate promoters from arabidopsis based on their transcript levels (meta-analysis of available microarray control datasets) to test their activity in comparison to the camv 35s promoter. a set of 11 candidate genes were identified which showed high transcript levels in t ...201424072400
the cotton transcription factor tcp14 functions in auxin-mediated epidermal cell differentiation and elongation.plant-specific teosinte-branched1/cycloidea/pcf (tcp) transcription factors play crucial roles in development, but their functional mechanisms remain largely unknown. here, we characterized the cellular functions of the class i tcp transcription factor ghtcp14 from upland cotton (gossypium hirsutum). ghtcp14 is expressed predominantly in fiber cells, especially at the initiation and elongation stages of development, and its expression increased in response to exogenous auxin. induced heterologou ...201323715527
wuschel overexpression promotes somatic embryogenesis and induces organogenesis in cotton (gossypium hirsutum l.) tissues cultured in vitro.this work shows that overexpression of the wus gene from arabidopsis enhanced the expression of embryogenic competence and triggered organogenesis from some cells of the regenerated embryo-like structures. agrobacterium-mediated genetic transformation of cotton was described in the late 1980s, but is still time consuming and largely genotype dependant due to poor regeneration. to help solve this bottleneck, we over-expressed the wuschel (wus) gene, a homeobox transcription factor cloned in arabi ...201323543366
new insights into roles of cell wall invertase in early seed development revealed by comprehensive spatial and temporal expression patterns of ghcwin1 in cotton.despite substantial evidence on the essential roles of cell wall invertase (cwin) in seed filling, it remains largely unknown how cwin exerts its regulation early in seed development, a critical stage that sets yield potential. to fill this knowledge gap, we systematically examined the spatial and temporal expression patterns of a major cwin gene, ghcwin1, in cotton (gossypium hirsutum) seeds from prefertilization to prestorage phase. ghcwin1 messenger rna was abundant at the innermost seed coat ...201222864582
activation of arabidopsis seed hair development by cotton fiber-related genes.each cotton fiber is a single-celled seed trichome or hair, and over 20,000 fibers may develop semi-synchronously on each seed. the molecular basis for seed hair development is unknown but is likely to share many similarities with leaf trichome development in arabidopsis. leaf trichome initiation in arabidopsis thaliana is activated by glabrous1 (gl1) that is negatively regulated by triptychon (try). using laser capture microdissection and microarray analysis, we found that many putative myb tra ...201121779324
identification of a udp-glucose pyrophosphorylase from cotton (gossypium hirsutum l.) involved in cellulose biosynthesis in arabidopsis thaliana.udp-glucose pyrophosphorylase is an important regulatory enzyme for the development of plants and a critical enzyme in synthesis of glycogen. here, we reported the cloning of a full-length ugp cdna from cotton, named ghugp. real-time pcr analysis indicated the ghugp expression in root, stem, leaf and flower of cotton, with a higher level in flower and root. the transcription level of ghugp depended on sucrose and light in short time and increased under low temperature, but decreased in o(2) defi ...201121373794
molecular cloning and characterization of ghapm, a gene encoding the μ subunit of the clathrin-associated adaptor protein complex that is associated with cotton (gossypium hirsutum) fiber development.the clathrin-associated adaptor protein (ap) complexes are the primary clathrin adaptors that contribute to the formation of clathrin-coated vesicles (ccvs). the ghapm gene (genbank accession number: gu359054), which encodes the medium subunit of the ap complexes, was cloned from cotton by rapid amplification of cdna ends-polymerase chain reaction (race-pcr). the full-length cdna was 1590 bp in size and encoded an open reading frame (orf) of 416 amino acids with a molecular weight of 46 kda. the ...201121225463
visualization of lipid droplet composition by direct organelle mass spectrometry.an expanding appreciation for the varied functions of neutral lipids in cellular organisms relies on a more detailed understanding of the mechanisms of lipid production and packaging into cytosolic lipid droplets (lds). conventional lipid profiling procedures involve the analysis of tissue extracts and consequently lack cellular or subcellular resolution. here, we report an approach that combines the visualization of individual lds, microphase extraction of lipid components from droplets, and th ...201121118810
a draft physical map of a d-genome cotton species (gossypium raimondii).genetically anchored physical maps of large eukaryotic genomes have proven useful both for their intrinsic merit and as an adjunct to genome sequencing. cultivated tetraploid cottons, gossypium hirsutum and g. barbadense, share a common ancestor formed by a merger of the a and d genomes about 1-2 million years ago. toward the long-term goal of characterizing the spectrum of diversity among cotton genomes, the worldwide cotton community has prioritized the d genome progenitor gossypium raimondii ...201020569427
apyrase (nucleoside triphosphate-diphosphohydrolase) and extracellular nucleotides regulate cotton fiber elongation in cultured ovules.ectoapyrase enzymes remove the terminal phosphate from extracellular nucleoside tri- and diphosphates. in arabidopsis (arabidopsis thaliana), two ectoapyrases, atapy1 and atapy2, have been implicated as key modulators of growth. in fibers of cotton (gossypium hirsutum), transcript levels for ghapy1 and ghapy2, two closely related ectoapyrases that have high sequence similarity to atapy1 and atapy2, are up-regulated when fibers enter their rapid growth phase. in an ovule culture system, fibers re ...201020018604
transcriptional profiling of mature arabidopsis trichomes reveals that noeck encodes the mixta-like transcriptional regulator myb106.leaf hairs (trichomes) of arabidopsis (arabidopsis thaliana) have been extensively used as a model to address general questions in cell and developmental biology. here, we lay the foundation for a systems-level understanding of the biology of this model cell type by performing genome-wide gene expression analyses. we have identified 3,231 genes that are up-regulated in mature trichomes relative to leaves without trichomes, and we compared wild-type trichomes with two mutants, glabra3 and triptyc ...200818805951
sequence analysis and characterization of vacuolar-type h+ -atpase proteolipid transcript from acanthus ebracteatus vah1.the vacuolar-type h+ -atpase (v-atpase) is a multimeric enzyme with diverse functions in plants such as nutrient transport, flowering, stress tolerance, guard cell movement and development. a partial sequence of v-atpase proteolipid was identified among the expressed sequence tags (ests) generated from acanthus ebracteatus, and selected for full-length sequencing. the 876-nucleotide cdna consists of an open reading frame of 165 amino acids. the deduced amino acid sequence displays high similarit ...200817852357
molecular cloning and characterization of crmdr1, a novel mdr-type abc transporter gene from catharanthus roseus.a novel gene encoding a mdr-like abc transporter protein was cloned from catharanthus roseus, a medicinal plant with more than 120 kinds of secondary metabolites, through rapid amplification of cdna ends (race). this gene (named as crmdr1; genbank accession no.: dq660356) had a total length of 4395 bp with an open reading frame of 3801 bp, and encoded a predicted polypeptide of 1266 amino acids with a molecular weight of 137.1 kda. the crmdr1 protein shared 59.8, 62.5, 60.0 and 58.2% identity wi ...200717541839
the gh3 family in plants: genome wide analysis in rice and evolutionary history based on est analysis.the gh3 gene family in arabidopsis, implicated in hormonal homeostasis through the conjugation of indolacetic and jasmonic acids to amino acids, is involved in a broad range of plant growth and development processes. in this work, the analysis of the gh3 family in the genome of oryza sativa identified 13 hypothetical orfs. est analysis and rt-pcr assays demonstrated that 12 of them were active genes. an extensive est analysis of the gh3 family performed on 26 plant species was used to estimate t ...200616488558
an internal motor kinesin is associated with the golgi apparatus and plays a role in trichome morphogenesis in arabidopsis.members of the kinesin superfamily are microtubule-based motor proteins that transport molecules/organelles along microtubules. we have identified similar internal motor kinesins, kinesin-13a, from the cotton gossypium hirsutum and arabidopsis thaliana. their motor domains share high degree of similarity with those of internal motor kinesins of animals and protists in the mcak/kinesin13 subfamily. however, no significant sequence similarities were detected in sequences outside the motor domain. ...200515574882
widespread paleopolyploidy in model plant species inferred from age distributions of duplicate genes.it is often anticipated that many of today's diploid plant species are in fact paleopolyploids. given that an ancient large-scale duplication will result in an excess of relatively old duplicated genes with similar ages, we analyzed the timing of duplication of pairs of paralogous genes in 14 model plant species. using est contigs (unigenes), we identified pairs of paralogous genes in each species and used the level of synonymous nucleotide substitution to estimate the relative ages of gene dupl ...200415208399
comparative proteomics of cannabis sativa plant tissues.comparative proteomics of leaves, flowers, and glands of cannabis sativa have been used to identify specific tissue-expressed proteins. these tissues have significantly different levels of cannabinoids. cannabinoids accumulate primarily in the glands but can also be found in flowers and leaves. proteins extracted from glands, flowers, and leaves were separated using two-dimensional gel electrophoresis. over 800 protein spots were reproducibly resolved in the two-dimensional gels from leaves and ...200415190082
identification of ghmyb109 encoding a r2r3 myb transcription factor that expressed specifically in fiber initials and elongating fibers of cotton (gossypium hirsutum l.).cotton (gossypium hirsutum l.) fibers are derived from ovule epidermis, which are developmentally similar to arabidopsis trichome where several myb transcription factors have been shown to control their formation. however, little is known about the molecular control of cotton fiber initiation. here we isolated 55 cotton myb domain-containing sequences expressed in ovules during fiber initiation. among them, ghmyb109, encoding a r2r3 myb transcription factor of 234 amino acids, was found to be st ...200314580676
[cloning and characterization of a lrr resistance like (ghlrr-rl) protein gene from cotton (gossypium hirsutum l.)].lrr (leucine rich repeat) proteins are involved in various biological processes. race was used to extend the 5' and 3' unknown sequence of a cdna-aflp fragment from cotton ovule, which had sequence similarity to the arabidopsis thaliana lrr resistance-like (lrr-rl) protein. by joining the sequence of cdna-aflp fragment and its 3' and 5' race product, it was found that the full-length cdna (genbank accession no. ay040532) of 1259 bp contained an orf of 987 bp and a putative poly(a) signal. the de ...200212143317
sequence and rt-pcr expression analysis of two peroxidases from arabidopsis thaliana belonging to a novel evolutionary branch of plant peroxidases.cdna clones encoding two new arabidopsis thaliana peroxidases, atp 1a and atp 2a, have been identified by searching the arabidopsis database of expressed sequence tags (dbest). they represent a novel branch of hitherto uncharacterized plant peroxidases which is only 35% identical in amino acid sequence to the well characterized group of basic plant peroxidases represented by the horseradish (armoracia rusticana) isoperoxidases hrp c, hrp e5 and the similar arabidopsis isoperoxidases atp ca, atp ...19979132061
comparative host selection responses of specialist (helicoverpa assulta) and generalist (helicoverpa armigera) moths in complex plant environments.we tested the behavioral responses of ovipositing females and natal larvae of two sibling species, a generalist helicoverpa armigera (hübner) and a specialist helicoverpa assulta (guenée), to odor sources emitted from different combinations of six plant species (tobacco, nicotiana tabacum; hot pepper, capsicum annuum; tomato, solanum esculentum; cotton, gossypium hirsutum; peanut, arachis hypogaea; maize, zea mays). under the conditions of plant materials versus corresponding controls, both stag ...201728182679
chlorophyll fluorescence induction kinetics and yield responses in rainfed crops with variable potassium nutrition in k deficient semi-arid alfisols.optimum potassium (k) nutrition in semi-arid regions may help crop plants to overcome constraints in their growth and development such as moisture stress, leading to higher productivity of rainfed crops, thus judicious k management is essential. a study was conducted to evaluate the importance of k nutrition on physiological processes like photosynthesis through chlorophyll a fluorescence and chlorophyll fluorescence induction kinetics (ojip) of rainfed crops viz., maize (zea mays l.), pearl mil ...201627101276
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