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functional divergence of ghcfe5 homoeologs revealed in cotton fiber and arabidopsis root cell development.in ghcfe5 homoeologs, ghcfe5d interacted with more actin homologs and stronger interaction activity than ghcfe5a. ghcfe5d - but not ghcfe5a -overexpression severely disrupted actin cytoskeleton organization and significantly suppressed cell elongation. homoeologous genes are common in polyploid plants; however, their functional divergence is poorly elucidated. allotetraploid upland cotton (gossypium hirsutum, aadd) is the most widely cultivated cotton; accounting for more than 90 % of the world' ...201626759310
functional characterization of ghsoc1 and ghmads42 homologs from upland cotton (gossypium hirsutum l.).in arabidopsis flowering pathway, mads-box genes encode transcription factors, with their structures and functions highly conserved in many species. in our study, two mads-box genes ghsoc1 and ghmads42 (gossypium hirsutum l.) were cloned from upland cotton ccri36 and transformed into arabidopsis. ghsoc1 was additionally transformed into upland cotton. comparative analysis demonstrated sequence conservation between ghsoc1 and ghmads42 and genes of other plant species. tissue-specific expression a ...201626566835
genome-scale analysis of the cotton kcs gene family revealed a binary mode of action for gibberellin a regulated fiber growth.production of β-ketoacyl-coa, which is catalyzed by 3-ketoacyl-coa synthase (kcs), is the first step in very long chain fatty acid (vlcfa) biosynthesis. here we identified 58 kcs genes from gossypium hirsutum, 31 from g. arboreum and 33 from g. raimondii by searching the assembled cotton genomes. the gene family was divided into the plant-specific fae1-type and the more general elo-type. kcs transcripts were widely expressed and 32 of them showed distinct subgenome-specific expressions in one or ...201626399709
coupling of mic-3 overexpression with the chromosomes 11 and 14 root-knot nematode (rkn) (meloidogyne incognita) resistance qtls provides insights into the regulation of the rkn resistance response in upland cotton (gossypium hirsutum).genetic analysis of mic-3 transgene with rkn resistance qtls provides insight into the resistance regulatory mechanism and provides a framework for testing additional hypotheses. resistance to root-knot nematode (rkn) (meloidogyne incognita) in upland cotton (gossypium hirsutum) is mediated by two major quantitative trait loci (qtl) located on chromosomes 11 and 14. the mic-3 (meloidogyne induced cotton3) protein accumulates specifically within the immature galls of rkn-resistant plants that pos ...201627314265
identification and characterization of micrornas in the plant parasitic root-knot nematode meloidogyne incognita using deep sequencing.the root-knot nematode meloidogyne incognita is among the most damaging plant-parasitic pests of several crops including cotton (gossypium hirsutum) and tomato (lycopersicon escultentum). recently, a genome has become available for m. incognita, which greatly facilitates investigation of the interactions between m. incognita and its plant hosts at the molecular level and enables formation of hypotheses concerning development at the cellular level. micrornas (mirnas) are a class of small rna mole ...201626743520
cotton (gossypium hirsutum l.) boll rotting bacteria vectored by the brown stink bug, euschistus servus (say) (hemiptera: pentatomidae).to determine the capacity of the brown stink bug (bsb) (euschistus servus) to transmit an infective pantoea agglomerans strain sc 1-r into cotton (gossypium hirsutum) bolls.201627261673
identification and evidence of positive selection upon resistance gene analogs in cotton (gossypium hirsutum l.).upland cotton (gossypium hirsutum l.) is an important fiber crop species, which is intensively plagued by a plethora of phytopathogenic fungi such as fusarium oxysporum f. sp. vasinfectum (fov) causing severe wilt disease. resistance gene analogs (rgas) are the largest class of potential resistance (r) genes depicting highly conserved domains and structures in plants. additionally, rgas are pivotal components of breeding projects towards host disease resistance, serving as useful functional mark ...201627729728
effects of silicon treatment and inoculation with fusarium oxysporum f. sp. vasinfectum on cellular defences in root tissues of two cotton cultivars.silicon has been shown to enhance the resistance of plants to fungal and bacterial pathogens. here, the effect of potassium silicate was assessed on two cotton (gossypium hirsutum) cultivars subsequently inoculated with fusarium oxysporum f. sp. vasinfectum (fov). sicot 189 is moderately resistant whilst sicot f-1 is the second most resistant commercial cultivar presently available in australia.201627288509
the fungal endophyte chaetomium globosum negatively affects both above- and belowground herbivores in cotton.mutualistic plant-endophyte symbioses can benefit plants by increasing host fitness through reductions in herbivory. the fungus, chaetomium globosum strain tamu 520, was previously isolated as an endophyte from cotton (gossypium hirsutum) and can be re-inoculated to systemically colonize cotton plants via seed treatment. we evaluated the potential impacts of the endophyte in cotton on plant parasitic nematodes belowground, along with piercing-sucking and chewing insects aboveground. endophytic c ...201627451418
cotton leaf curl multan virus-derived viral small rnas can target cotton genes to promote viral infection.rna silencing is a conserved mechanism in plants that targets viruses. viral small rnas (vsirnas) can be generated from viral double-stranded rna replicative intermediates within the infected host, or from host rna-dependent rna polymerases activity on viral templates. the abundance and profile of vsirnas in viral infections have been reported previously. however, the involvement of vsirnas during infection of the geminiviridae family member cotton leaf curl virus (clcud), which causes significa ...201627540385
interaction of transgenic and natural insect resistance mechanisms against spodoptera littoralis in cotton.insect-resistant transgenic plants that express insecticidal cry proteins from bacillus thuringiensis (bt) are grown on millions of hectares worldwide. while these proteins are efficient in controlling key lepidopteran pests, not all pests are affected and the development of resistance in target pests is always a concern. these shortcomings could be addressed by exploiting the natural insect resistance of cotton, especially inducible terpenoids such as gossypol.201628019063
high expression of cry1ac protein in cotton (gossypium hirsutum) by combining independent transgenic events that target the protein to cytoplasm and plastids.transgenic cotton was developed using two constructs containing a truncated and codon-modified cry1ac gene (1,848 bp), which was originally characterized from bacillus thuringiensis subspecies kurstaki strain hd73 that encodes a toxin highly effective against many lepidopteran pests. in construct i, the cry1ac gene was cloned under fmvde, a strong constitutively expressing promoter, to express the encoded protein in the cytoplasm. in construct ii, the encoded protein was directed to the plastids ...201627391960
assessment of bt trait purity in different generations of transgenic cottons.adequate expression of bt (bacillus thuringiensis) toxins and purity of seeds of bt-transgenic cottons are important for controlling bollworms, and thereby increasing the cotton productivity. therefore, we examined the variability in expression of bt toxin proteins in the seeds and in leaves of different cotton (gossypium hirsutum (l.) hybrids (jkch 226, jkch 1947, jkch durga, jkch ishwar, jkch varun kdchh 441 and kdchh 621) expressing bt toxins in f₁ and f₂ generations, using bioassays against ...201627295920
ghataf1, a nac transcription factor, confers abiotic and biotic stress responses by regulating phytohormonal signaling networks.dual function of ghataf1 in the responses to salinity stress and verticillium dahliae infection in cotton. nac (nam/ataf1/2/cuc2) is a large plant-specific transcription factor family that plays important roles in the response to abiotic stresses. we previously isolated a cotton nac transcription factor gene, ghataf1, which was up-regulated by aba, cold and salt stresses and classified into afat1/2, a sub-family of nac. here, we report that ghataf1 was also highly induced by meja, sa and vertici ...201627432176
ghwrky25, a group i wrky gene from cotton, confers differential tolerance to abiotic and biotic stresses in transgenic nicotiana benthamiana.wrky transcription factors are involved in various processes, ranging from plant growth to abiotic and biotic stress responses. group i wrky members have been rarely reported compared with group ii or iii members, particularly in cotton (gossypium hirsutum). in this study, a group i wrky gene, namely, ghwrky25, was cloned from cotton and characterized. expression analysis revealed that ghwrky25 can be induced or deduced by the treatments of abiotic stresses and multiple defense-related signaling ...201626410829
structure of exogenous gene integration and event-specific detection in the glyphosate-tolerant transgenic cotton line bg2-7.in this study, the flanking sequence of an inserted fragment conferring glyphosate tolerance on transgenic cotton line bg2-7 was analyzed by thermal asymmetric interlaced polymerase chain reaction (tail-pcr) and standard pcr. the results showed apparent insertion of the exogenous gene into chromosome d10 of the gossypium hirsutum l. genome, as the left and right borders of the inserted fragment are nucleotides 61,962,952 and 61,962,921 of chromosome d10, respectively. in addition, a 31-bp cotton ...201627379683
overexpression of cotton ghmpk11 decreases disease resistance through the gibberellin signaling pathway in transgenic nicotiana benthamiana.many changes in development, growth, hormone activity and environmental stimuli responses are mediated by mitogen-activated protein kinase (mapk) cascades. however, in plants, studies on mapks have mainly focused on mpk3, mpk4 and mpk6. here, a novel group b mapk gene, ghmpk11, was isolated from cotton (gossypium hirsutum l.) and characterized. both promoter and expression pattern analyses revealed that ghmpk11 is involved in defense responses and signaling pathways. ghmpk11 overexpression in ni ...201627242882
fine mapping and identification of candidate genes for a qtl affecting meloidogyne incognita reproduction in upland cotton.the southern root-knot nematode (meloidogyne incognita; rkn) is one of the most important economic pests of upland cotton (gossypium hirsutum l.). host plant resistance, the ability of a plant to suppress nematode reproduction, is the most economical, practical, and environmentally sound method to provide protection against this subterranean pest. the resistant line auburn 623rnr and a number of elite breeding lines derived from it remain the most important source of root-knot nematode (rkn) res ...201627503539
the cotton myb108 forms a positive feedback regulation loop with cml11 and participates in the defense response against verticillium dahliae infection.accumulating evidence indicates that plant myb transcription factors participate in defense against pathogen attack, but their regulatory targets and related signaling processes remain largely unknown. here, we identified a defense-related myb gene (ghmyb108) from upland cotton (gossypium hirsutum) and characterized its functional mechanism. expression of ghmyb108 in cotton plants was induced by verticillium dahliae infection and responded to the application of defense signaling molecules, inclu ...201626873979
evaluation of brevibacillus brevis as a potential plant growth promoting rhizobacteria for cotton (gossypium hirsutum) crop.the present investigation was undertaken to isolate, screen and evaluate a selected promising pgpr brevibacillus brevis on cotton crop. out of 156 bacterial isolates one of the most promising isolate was analyzed for the various pgp traits. a seed germination analysis was conducted with cotton seeds to evaluate the potential of the isolate to promote plant growth. the bacterial isolate was checked for its growth and survival at high temperatures. the isolate was also analyzed for the pgp traits ...201627386392
development of cotton leaf curl virus resistant transgenic cotton using antisense ßc1 gene.cotton leaf curl virus (clcuv) is a serious pathogen causing leaf curl disease and affecting the cotton production in major growing areas. the transgenic cotton (gossypium hirsutum cv. coker 310) plants were developed by using βc1 gene in antisense orientation gene driven by cauliflower mosaic virus-35s promoter and nos (nopaline synthase) terminator and mediated by agrobacterium tumefaciens transformation and somatic embryogenesis system. molecular confirmation of the transformants was carried ...201627081361
rnai-mediated resistance against cotton leaf curl disease in elite indian cotton (gossypium hirsutum) cultivar narasimha.cotton leaf curl disease (clcud) is caused by several distinct begomovirus species in association with disease-specific betasatellite essential for induction of disease symptoms. clcud is a serious threat for the cultivation of cotton (gossypium sp.) and several species in the family malvaceae. in this study, rnai-based approach was applied to generate transgenic cotton (gossypium hirsutum) plants resistant to cotton leaf curl rajasthan virus (clcurv). an intron hairpin (ihp) rnai construct capa ...201627028623
antisense expression of gossypium hirsutum udp-glucuronate decarboxylase in arabidopsis leads to changes in cell wall components.udp-glucuronate decarboxylase (udp-xylose synthase; uxs, ec 4.1.1.35) is an essential enzyme of the non-cellulosic polysaccharide biosynthetic pathway. in the present study, using transient expression of fluorescently labeled gossypium hirsutum uxs (ghuxs3) protein in onion epidermal cells, we observed that this protein was distributed in the cytoplasm. the ghuxs3 cdna of cotton was expressed in an antisense orientation in arabidopsis thaliana by agrobacterium tumefaciens-mediated transformation ...201626909959
constitutive expression of the tzs gene from agrobacterium tumefaciens virg mutant strains is responsible for improved transgenic plant regeneration in cotton meristem transformation.key message : virg mutant strains of a nopaline type of agrobacterium tumefaciens increase the transformation frequency in cotton meristem transformation. constitutive cytokinin expression from the tzs gene in the virg mutant strains is responsible for the improvement. strains of agrobacterium tumefaciens were tested for their ability to improve cotton meristem transformation frequency. two disarmed a. tumefaciens nopaline strains with either a virgn54d constitutively active mutation or virgi77v ...201626650837
inoculant of arbuscular mycorrhizal fungi (rhizophagus clarus) increase yield of soybean and cotton under field conditions.nutrient availability is an important factor in crop production, and regular addition of chemical fertilizers is the most common practice to improve yield in agrosystems for intensive crop production. the use of some groups of microorganisms that have specific activity providing nutrients to plants is a good alternative, and arbuscular mycorrhizal fungi (amf) enhance plant nutrition by providing especially phosphorus, improving plant growth and increasing crop production. unfortunately, the use ...201627303367
ghmcs1, the cotton orthologue of human grim-19, is a subunit of mitochondrial complex i and associated with cotton fibre growth.grim-19 (gene associated with retinoid-interferon-induced mortality 19) is a subunit of mitochondrial respiratory complex i in mammalian systems, and it has been demonstrated to be a multifunctional protein involved in the cell cycle, cell motility and innate immunity. however, little is known about the molecular functions of its homologues in plants. here, we characterised ghmcs1, an orthologue of human grim-19 from cotton (gossypium hirsutum l.), and found that it was essential for maintaining ...201627632161
identification of stable quantitative trait loci (qtls) for fiber quality traits across multiple environments in gossypium hirsutum recombinant inbred line population.the identification of quantitative trait loci (qtls) that are stable and consistent across multiple environments and populations plays an essential role in marker-assisted selection (mas). in the present study, we used 28,861 simple sequence repeat (ssr) markers, which included 12,560 gossypium raimondii (d genome) sequence-based ssr markers to identify polymorphism between two upland cotton strains 0-153 and sgk9708. a total of 851 polymorphic primers were finally selected and used to genotype ...201626951621
fluometuron and pendimethalin runoff from strip and conventionally tilled cotton in the southern atlantic coastal plain.in the atlantic coastal plain region of southern georgia (usa), cotton (gossypium hirsutum l.) acreage increased threefold in the past decade. to more effectively protect water quality in the region, best management practices are needed that reduce pesticide runoff from fields in cotton production. this study compared runoff of two herbicides, fluometuron [n,n-dimethyl-n'-[3-(trifluoromethyl)-phenyl]-urea] and pendimethalin [n-(1-ethylpropyl)-3,4-dimethyl-2,6-dinitro-benzenamine], from plots in ...201515537934
dose and time dependent efficacy alteration of different defoliants on seed cotton yield.field experiments were conducted during 2012 and 2013 to determine the effect of select defoliants i.e dropp ultra and ethrel, their optimal dose and suitable time of application on earliness and seed cotton yield in 3 american cotton cultivars. mrc7361bgii (3055.2 kg ha(-1)) and mrc7017bgii (2913.0 kg ha(-1)) resulted in significantly improved seed cotton yield as compared to f1 861 (2346.0 kg ha(-1)). dropp ultra? @ 200 ml ha(-1) revealed highest yield (3018.7 kg ha(-1)) followed by ethrel @ 2 ...201526364466
evaluation of different carbon sources for high frequency callus culture with reduced phenolic secretion in cotton (gossypium hirsutum l.) cv. svpr-2.an efficient protocol was developed to control excessive phenolic compound secretion during callus culture of cotton. as cotton is naturally rich in phenolic compounds factors influencing the phenolic compound secretion, callus induction and proliferation were optimized for getting high frequency callus culture. different carbon sources such as fructose, glucose, sucrose and maltose were tested at various concentrations to control phenolic secretion in callus culture. among them, 3% maltose was ...201528626717
effect of high-temperature stress on the growth and seed characteristics of barley and cotton.the existence of a sensitivity gradient to a uniform high-temperature stress applied at stages during seed maturation can be demonstrated in barley. the germination of freshly harvested seed is depressed following heat stress at 7--10 days after awn emergence, but is enhanced by the same stress applied 3 weeks after awn emergence. the depression is attributed to reduced viability associated with thermal injury. the stimulation following stress at more mature stages of seed development is related ...20151032107
genome-wide transcriptome analysis of cotton (gossypium hirsutum l.) identifies candidate gene signatures in response to aflatoxin producing fungus aspergillus flavus.aflatoxins are toxic and potent carcinogenic metabolites produced from the fungi aspergillus flavus and a. parasiticus. aflatoxins can contaminate cottonseed under conducive preharvest and postharvest conditions. united states federal regulations restrict the use of aflatoxin contaminated cottonseed at >20 ppb for animal feed. several strategies have been proposed for controlling aflatoxin contamination, and much success has been achieved by the application of an atoxigenic strain of a. flavus i ...201526366857
overexpression of 3-deoxy-7-phosphoheptulonate synthase gene from gossypium hirsutum enhances arabidopsis resistance to verticillium wilt.expression of dhs1 in cotton is induced upon infection by verticillium dahliae , and overexpression of ghdhs1 endows transgenic arabidopsis plants excellent verticillium resistance. verticillium wilt is caused by a soil-borne fungus verticillium dahliae. resistance in most cotton cultivars is either scarce or unavailable, making verticillium wilt a major obstacle in cotton production. here, we identified a 3-deoxy-7-phosphoheptulonate synthase (dhs, ec 4.1.2.15) gene from gossypium hirsutum, nam ...201525929795
identification of mirnas and their targets in cotton inoculated with verticillium dahliae by high-throughput sequencing and degradome analysis.micrornas (mirnas) are a group of endogenous small non-coding rnas that play important roles in plant growth, development, and stress response processes. verticillium wilt is a vascular disease in plants mainly caused by verticillium dahliae kleb., the soil-borne fungal pathogen. however, the role of mirnas in the regulation of verticillium defense responses is mostly unknown. this study aimed to identify new mirnas and their potential targets that are involved in the regulation of verticillium ...201526133244
pseudomonas induces salinity tolerance in cotton (gossypium hirsutum) and resistance to fusarium root rot through the modulation of indole-3-acetic acid.abiotic stresses cause changes in the balance of phytohormones in plants and result in inhibited root growth and an increase in the susceptibility of plants to root rot disease. the aim of this work was to ascertain whether microbial indole-3-acetic acid (iaa) plays a role in the regulation of root growth and microbially mediated control of root rot of cotton caused by fusarium solani. seed germination and seedling growth were improved by both nacl and mg2so4 (100 mm) solutions when treated with ...201526587006
functional characterization of a strong bi-directional constitutive plant promoter isolated from cotton leaf curl burewala virus.cotton leaf curl burewala virus (clcubuv), belonging to the genus begomovirus, possesses single-stranded monopartite dna genome. the bidirectional promoters representing rep and coat protein (cp) genes of clcubuv were characterized and their efficacy was assayed. rep and cp promoters of clcubuv and 35s promoter of cauliflower mosaic virus (camv) were fused with β-glucuronidase (gus) and green fluorescent protein (gfp) reporter genes. gus activity in individual plant cells driven by rep, cp and 3 ...201525799504
transformation and evaluation of cry1ac+cry2a and gtgene in gossypium hirsutum l.more than 50 countries around the globe cultivate cotton on a large scale. it is a major cash crop of pakistan and is considered "white gold" because it is highly important to the economy of pakistan. in addition to its importance, cotton cultivation faces several problems, such as insect pests, weeds, and viruses. in the past, insects have been controlled by insecticides, but this method caused a severe loss to the economy. however, conventional breeding methods have provided considerable break ...201526617613
fall armyworm (lepidoptera: noctuidae) development, survivorship, and damage on cotton plants expressing insecticidal plant-incorporated protectants.cotton, gossypium hirsutum (l.), plants expressing insecticidal crystal (cry) proteins of bacillus thuringiensis (bt) berliner are planted on significant acreage across the southern region of the united states. fall armyworm, spodoptera frugiperda (j. e. smith), can be a significant cotton pest in some years, but this species has not been a primary target of bt cotton technologies. the objective of this study was to quantify fall armyworm larval survivorship and fruiting form injury on transgeni ...201526470233
cadherin is involved in the action of bacillus thuringiensis toxins cry1ac and cry2aa in the beet armyworm, spodoptera exigua.bacillus thuringiensis (bt) insecticidal crystal (cry) proteins are effective against some insect pests in sprays and transgenic crops, although the evolution of resistance could threaten the long-term efficacy of such bt use. one strategy to delay resistance to bt crops is to "pyramid" two or more bt proteins that bind to distinct receptor proteins within the insect midgut. the most common bt pyramid in cotton (gossypium hirsutum l.) employs cry1ac with cry2ab to target several key lepidopteran ...201525754522
asiatic cotton can generate similar economic benefits to bt cotton under rainfed conditions.american cotton (gossypium hirsutum l.), transformed with bacillus thuringiensis cry genes (bt g. hirsutum) that confer resistance to lepidopteran pests, is extensively cultivated worldwide. in india, transgenic bt g. hirsutum was commercially released in 2002 and by 2014 95% of farmers had adopted bt g. hirsutum(1). the economic benefits of bt g. hirsutum over non-bt g. hirsutum are well documented and include increase in yields, increase in farmers' net revenue and reduction in pesticide appli ...201527250007
high genetic homogeneity points to a single introduction event responsible for invasion of cotton leaf curl multan virus and its associated betasatellite into china.cotton leaf curl multan virus (clcumuv) is a whitefly transmitted geminivirus (wtg) endemic to the india subcontinent and is notorious as a causal agent of cotton leaf curl disease (clcud), a major constraint to cotton production in south asia. we found clcumuv infecting hibiscus rosa-sinensis in guangzhou, china in 2006. the spread and evolution of the invading clcumuv were monitored in the following nine years.201526445958
functional characterization of an anthocyanidin reductase gene from the fibers of upland cotton (gossypium hirsutum).metabolic profiling, gene cloning, enzymatic analysis, ectopic expression, and gene silencing experiments demonstrate that the anthocyanidin reductase (anr) pathway is involved in the biosynthesis of proanthocyanidins in upland cotton. proanthocyanidins (pas) are oligomeric or polymeric flavan-3-ols, however, the biosynthetic pathway of pas in cotton remains to be elucidated. here, we report on an anthocyanidin reductase (anr) gene from cotton fibers and the anr pathway of pas. phytochemical ana ...201525575669
sequence composition of bac clones and ssr markers mapped to upland cotton chromosomes 11 and 21 targeting resistance to soil-borne pathogens.genetic and physical framework mapping in cotton (gossypium spp.) were used to discover putative gene sequences involved in resistance to common soil-borne pathogens. chromosome (chr) 11 and its homoeologous chr 21 of upland cotton (g. hirsutum) are foci for discovery of resistance (r) or pathogen-induced r (pr) genes underlying qtls involved in response to root-knot nematode (meloidogyne incognita), reniform nematode (rotylenchulus reniformis), fusarium wilt (fusarium oxysporum f.sp. vasinfectu ...201526483808
analyses of fusarium wilt race 3 resistance in upland cotton (gossypium hirsutum l.).fusarium wilt [fusarium oxysporum f.sp. vasinfectum (fov) atk. sny & hans] represents a serious threat to cotton (gossypium spp.) production. for the last few decades, the fov pathogen has become a significant problem in uzbekistan causing severe wilt disease and yield losses of g. hirsutum l. cultivars. we present the first genetic analyses of fov race 3 resistance on uzbek cotton germplasm with a series of field and greenhouse artificial inoculation-evaluations and inheritance studies. the fie ...201525896369
relationship between piercing-sucking insect control and internal lint and seed rot in southeastern cotton (gossypium hirsutum l.).in 1999, crop consultants scouting for stink bugs (hemiptera spp.) in south carolina discovered a formerly unobserved seed rot of cotton that caused yield losses ranging from 10 to 15% in certain fields. the disease has subsequently been reported in fields throughout the southeastern cotton belt. externally, diseased bolls appeared undamaged; internally, green fruit contain pink to dark brown, damp, deformed lint, and necrotic seeds. in greenhouse experiments, we demonstrated transmission of the ...201526470293
bacillus gossypii sp. nov., isolated from the stem of gossypium hirsutum.a gram-stain-positive, facultatively anaerobic, endospore-forming organism, isolated from the stem of gossypium hirsutum, was studied to determine its taxonomic position. on the basis of 16s rrna gene sequence similarity comparisons, strain jm-267t was grouped in the genus bacillus, related most closely to the type strains of bacillus simplex and bacillus huizhouensis (both 97.8%), bacillus muralis (97.7%), bacillus butanolivorans and bacillus psychrosaccharolyticus (both 97.3%). 16s rrna gene s ...201526303844
overexpression of mic-3 indicates a direct role for the mic gene family in mediating upland cotton (gossypium hirsutum) resistance to root-knot nematode (meloidogyne incognita).transgene-based analysis of the mic-3 gene provides the first report of a cotton gene having a direct role in mediating cotton resistance to root-knot nematode. major quantitative trait loci have been mapped to upland cotton (gossypium hirsutum l.) chromosomes 11 and 14 that govern the highly resistant phenotype in response to infection by root-knot nematode (rkn; meloidogyne incognita); however, nearly nothing is known regarding the underlying molecular determinants of this rkn-resistant phenot ...201525376794
a novel nap member ghnap is involved in leaf senescence in gossypium hirsutum.premature leaf senescence has a negative influence on the yield and quality of cotton, and several genes have been found to regulate leaf senescence. howeer, many underlying transcription factors are yet to be identified. in this study, a nap-like transcription factor (ghnap) was isolated from gossypium hirsutum. ghnap has the typical nac structure and a conserved novel subdomain in its divergent transcription activation region (tar). ghnap was demonstrated to be a nuclear protein, and it showed ...201525991739
analyzing serial cdna libraries revealed reactive oxygen species and gibberellins signaling pathways in the salt response of upland cotton (gossypium hirsutum l.).by comparing series full-length cdna libraries stressed and control, the dynamic process of salt stress response in upland cotton was studied, and reactive oxygen species and gibberellins signaling pathways were proposed. the upland cotton is the most important fiber plant with highly salt tolerance. however, the molecular mechanism underlying salt tolerance in domesticated cotton was unclear. here, seven full-length cdna libraries were constructed for seedling roots of upland cotton 'zhong g 5' ...201525700980
steam explosion distinctively enhances biomass enzymatic saccharification of cotton stalks by largely reducing cellulose polymerization degree in g. barbadense and g. hirsutum.in this study, steam explosion pretreatment was performed in cotton stalks, leading to 5-6 folds enhancements on biomass enzymatic saccharification distinctive in gossypium barbadense and gossypium hirsutum species. sequential 1% h2so4 pretreatment could further increase biomass digestibility of the steam-exploded stalks, and also cause the highest sugar-ethanol conversion rates probably by releasing less inhibitor to yeast fermentation. by comparison, extremely high concentration alkali (16% na ...201525656866
quantitative proteomics and transcriptomics reveal key metabolic processes associated with cotton fiber initiation.an itraq-based proteomics of ovules from the upland cotton species gossypium hirsutum and its fuzzless-lintless mutant was performed, and finally 2729 proteins that preferentially accumulated at anthesis in wild-type ovules were identified. we confirmed that the gene expression levels of 2005 among these proteins also increased by performing an rna sequencing transcriptomics. expression of proteins involved in carboxylic acid metabolism, small-molecule metabolic processes, hormone regulation, an ...201525449837
variovorax gossypii sp. nov., isolated from gossypium hirsutum.a beige-pigmented bacterial strain (jm-310t), isolated from the healthy internal root tissue of 4-week-old cotton (gossypium hirsutum, cultivar 'des-119') in tallassee (macon county), alabama, usa, was studied taxonomically. the isolate produced small rod-shaped cells, which showed a gram-negative staining behaviour. a comparison of the 16s rrna gene sequence of the isolate revealed 99.2, 98.8, 98.7, 98.7, 98.1 and 97.6 % similarity to the 16s rrna gene sequences of the type strains of variovora ...201526341669
postinfection development of rotylenchulus reniformis on resistant gossypium barbadense accessions.the reniform nematode (rotylenchulus reniformis) causes significant cotton (gossypium hirsutum) losses in the southeastern united states. the research objective was to describe the effects of two resistant g. barbadense lines (cultivar tx 110 and accession gb 713) on development and fecundity of reniform nematode. nematode development and fecundity were evaluated on the resistant lines and susceptible g. hirsutum cultivar deltapine 16 in three repeated growth chamber experiments. nematode develo ...201526941458
transcriptome analysis of cotton (gossypium hirsutum l.) genotypes that are susceptible, resistant, and hypersensitive to reniform nematode (rotylenchulus reniformis).reniform nematode is a semi-endoparasitic nematode species causing significant yield loss in numerous crops, including cotton (gossypium hirsutum l.). an rna-sequencing analysis was conducted to measure transcript abundance in reniform nematode susceptible (dp90 & sg747), resistant (barbren-713), and hypersensitive (lonren-1) genotypes of cotton (gossypium hirsutum l.) with and without reniform nematode infestation. over 90 million trimmed high quality reads were assembled into 84,711 and 80, 35 ...201526571375
interference between redroot pigweed (amaranthus retroflexus l.) and cotton (gossypium hirsutum l.): growth analysis.redroot pigweed is one of the injurious agricultural weeds on a worldwide basis. understanding of its interference impact in crop field will provide useful information for weed control programs. the effects of redroot pigweed on cotton at densities of 0, 0.125, 0.25, 0.5, 1, 2, 4, and 8 plants m(-1) of row were evaluated in field experiments conducted in 2013 and 2014 at institute of cotton research, caas in china. redroot pigweed remained taller and thicker than cotton and heavily shaded cotton ...201526057386
interspecific associations between cycloneda sanguinea and two aphid species (aphis gossypii and hyadaphis foeniculi) in sole-crop and fennel-cotton intercropping systems.aphids cause significant damage to crop plants. studies regarding predator-prey relationships in fennel (foeniculum vulgare mill.) and cotton (gossypium hirsutum l.) crops are important for understanding essential ecological interactions in the context of intercropping and for establishing pest management programs for aphids. this study evaluated the association among hyadaphis foeniculi (passerini) (hemiptera: aphididae), aphis gossypii glover (hemiptera: aphididae) and cycloneda sanguinea (l.) ...201526241862
host-associated differentiation in a highly polyphagous, sexually reproducing insect herbivore.insect herbivores may undergo genetic divergence on their host plants through host-associated differentiation (had). much of what we know about had involves insect species with narrow host ranges (i.e., specialists) that spend part or all their life cycle inside their hosts, and/or reproduce asexually (e.g., parthenogenetic insects), all of which are thought to facilitate had. however, sexually reproducing polyphagous insects can also exhibit had. few sexually reproducing insects have been teste ...201526257868
molecular cloning and expression analysis of five ghraxs in upland cotton (gossypium hirsutum l.).the formation of axillary meristems in leaf axils is a prerequisite for the development of lateral shoots, which largely contribute to plant architecture. several transcription factor-encoding genes, including cuc3, rax, las, lof1, and rox, have been cloned by screening for axillary meristem mutants in arabidopsis thaliana. these genes will facilitate our understanding of the mechanisms underlying axillary meristem development. in this study, we report the cloning of five genes from cotton (goss ...201526505359
kinesin-4 functions in vesicular transport on cortical microtubules and regulates cell wall mechanics during cell elongation in plants.in plants, anisotropic cell expansion depends on cortical microtubules that serve as tracks along which macromolecules and vesicles are transported by the motor kinesins of unknown identities. we used cotton (gossypium hirsutum) fibers that underwent robust elongation to discover kinesins that are involved in cell elongation and found gh kinesin-4a expressed abundantly. the motor was detected by immunofluorescence on vesicle-like structures that were associated with cortical microtubules. in ara ...201525600279
temperature responses of mesophyll conductance differ greatly between species.the temperature responses of mesophyll conductance (gm ) were investigated for nine species using carbon isotope techniques combining tunable diode laser spectroscopy and gas exchange measurements. species included the evergreen trees eucalyptus pauciflora and quercus engelmannii; the tropical evergreen tree lophostemon confertus; as well as the herbaceous species nicotiana tabacum, oryza sativa, triticum aestivum, gossypium hirsutum, glycine max and arabidopsis thaliana. responses varied from a ...201525224884
tillage impact on herbicide loss by surface runoff and lateral subsurface flow.there is worldwide interest in conservation tillage practices because they can reduce surface runoff, and agrichemical and sediment losses from farm fields. since these practices typically increase infiltration, their use may increase subsurface transport of water-soluble contaminants. thus, to assess long-term environmental benefits of conservation tillage data may be needed that quantify both surface and subsurface contaminant fluxes. this study focused on the herbicide fluometuron (n,n-dimeth ...201526057540
spatial and temporal dynamics of stink bugs in southeastern farmscapes.a 3-yr study (2009-2011) was conducted to examine the spatial and temporal dynamics of stink bugs in three commercial farmscapes. study locations were replicated in south carolina and georgia, in an agriculturally diverse region known as the southeastern coastal plain. crops included wheat, triticum aestivum (l.), corn, zea mays (l.), soybean, glycine max (l.), cotton, gossypium hirsutum (l.), and peanut, arachis hypogaea (l.). farmscapes were sampled weekly using whole-plant examinations for co ...201525843577
determining the major bt refuge crops for cotton bollworm in north china.evaluation of the effectiveness of refuge strategies involved in cotton bollworm bt resistance management would be aided by technologies that allow monitoring and quantification of key factors that affect the process under field conditions. we hypothesized that characterization of stable carbon and nitrogen isotopes in adult bollworm, helicoverpa armigera (hübner) (lepidoptera: noctuidae) moths may aid in determining the larval host that they developed upon. we found moths reared from larvae fed ...201525196072
molecular cloning of a dehydration-responsive protein gene (mrd22) from mulberry, and determination of abiotic stress patterns of mrd22 gene expression.a full-length cdna sequence coding for dehydration-responsive protein gene of mulberry tree, which we designated was mrd22 (genbank accession number: jq804833) was cloned based on mulberry expressed sequence tags (ests). mrd22 is 1503 bp long, contains a 334 bp 5'-utr (untranslated region) and a 563 bp 3'-utr, encodes 201 amino acids with a predicted molecular weight of 54.28 kda and an isoelectric point of 9.35. phylogenetic analysis based on mrd22 sequences from different species showed that m ...201525895340
tillage, cropping systems, and nitrogen fertilizer source effects on soil carbon sequestration and fractions.quantification of soil carbon (c) cycling as influenced by management practices is needed for c sequestration and soil quality improvement. we evaluated the 10-yr effects of tillage, cropping system, and n source on crop residue and soil c fractions at 0- to 20-cm depth in decatur silt loam (clayey, kaolinitic, thermic, typic paleudults) in northern alabama, usa. treatments were incomplete factorial combinations of three tillage practices (no-till [nt], mulch till [mt], and conventional till [ct ...201518453410
lack of adaptation to a new host in a generalist herbivore: implications for host plant resistance to twospotted spider mites in cotton.the twospotted spider mite (tetranychus urticae koch) is an important pest of cotton. this pest has a broad host range, but when changing between hosts an initial decline in fitness often occurs. this is usually followed by an increase in fitness after rapid adaptation to the new host, usually within five generations.201524777962
dynamics of soil diazotrophic community structure, diversity, and functioning during the cropping period of cotton (gossypium hirsutum).the soil sampled at different growth stages along the cropping period of cotton were analyzed using various molecular tools: restriction fragment length polymorphism (rflp), terminal restriction length polymorphism (t-rflp), and cloning-sequencing. the cluster analysis of the diazotrophic community structure of early sampled soil (0, 15, and 30 days) was found to be more closely related to each other than the later sampled one. phylogenetic and diversity analysis of sequences obtained from the f ...201524677076
effects of climate change on phenological trends and seed cotton yields in oasis of arid regions.understanding the effects of climatic change on phenological phases of cotton (gossypium hirsutum l.) in oasis of arid regions may help optimize management schemes to increase productivity. this study assessed the impacts of climatic changes on the phenological phases and productivity of spring cotton. the results showed that climatic warming led the dates of sowing seed, seeding emergence, three-leaf, five-leaf, budding, anthesis, full bloom, cleft boll, boll-opening, boll-opening filling, and ...201525240389
non-destructive high-throughput dna extraction and genotyping methods for cotton seeds and seedlings.extensive use of targeted pcr-based genotyping is precluded for many plant research laboratories by the cost and time required for dna extraction. using cotton (gossypium hirsutum) as a model for plants with medium-sized seeds, we report here manual procedures for inexpensive non-destructive high-throughput extraction of dna suitable for pcr-based genotyping of large numbers of individual seeds and seedlings. by sampling only small amounts of cotyledon tissue of ungerminated seed or young seedli ...201525967902
is nectar reabsorption restricted by the stalk cells of floral and extrafloral nectary trichomes?reabsorption is a phase of nectar dynamics that occurs concurrently with secretion; it has been described in floral nectaries that exude nectar through stomata or unicellular trichomes, but has not yet been recorded in extrafloral glands. apparently, nectar reabsorption does not occur in multicellular secretory trichomes (mst) due to the presence of lipophilic impregnations - which resemble casparian strips - in the anticlinal walls of the stalk cells. it has been assumed that these impregnation ...201524987788
dirigent proteins from cotton (gossypium sp.) for the atropselective synthesis of gossypol.gossypol is a defense compound in cotton plants for protection against pests and pathogens. gossypol biosynthesis involves the oxidative coupling of hemigossypol and results in two atropisomers owing to hindered rotation around the central binaphthyl bond. (+)-gossypol predominates in vivo, thus suggesting stereochemically controlled biosynthesis. the aim was to identify the factors mediating (+)-gossypol formation in cotton and to investigate their potential for asymmetric biaryl synthesis. a d ...201526460165
novosphingobium gossypii sp. nov., isolated from gossypium hirsutum.a gram-stain-negative, rod-shaped, non-spore-forming bacterium (strain jm-1396(t)) producing a yellow pigment, was isolated from the healthy internal stem tissue of post-harvest cotton (gossypium hirsutum, cultivar 'des-119') grown at the plant breeding unit at the e.v. smith research center in tallassee (macon county), al, usa. 16s rrna gene sequence analysis of strain jm-1396(t) showed high sequence similarity values to the type strains of novosphingobium mathurense, novosphingobium panipatens ...201525985829
constructing a high-density linkage map for gossypium hirsutum × gossypium barbadense and identifying qtls for lint percentage.to introgress the good fiber quality and yield from gossypium barbadense into a commercial upland cotton variety, a high-density simple sequence repeat (ssr) genetic linkage map was developed from a bc1 f1 population of gossypium hirsutum × gossypium barbadense. the map comprised 2,292 loci and covered 5115.16 centimorgan (cm) of the cotton ad genome, with an average marker interval of 2.23 cm. of the marker order for 1,577 common loci on this new map, 90.36% agrees well with the marker order on ...201525263268
the genome sequence of sea-island cotton (gossypium barbadense) provides insights into the allopolyploidization and development of superior spinnable fibres.gossypium hirsutum contributes the most production of cotton fibre, but g. barbadense is valued for its better comprehensive resistance and superior fibre properties. however, the allotetraploid genome of g. barbadense has not been comprehensively analysed. here we present a high-quality assembly of the 2.57 gigabase genome of g. barbadense, including 80,876 protein-coding genes. the double-sized genome of the a (or at) (1.50 gb) against d (or dt) (853 mb) primarily resulted from the expansion o ...201526634818
data for proteomic profiling of anthers from a photosensitive male sterile mutant and wild-type cotton (gossypium hirsutum l.).cotton is an important economic crop, used mainly for the production of textile fiber. using a space mutation breeding technique, a novel photosensitive genetic male sterile mutant ccri9106 was isolated from the wild-type upland cotton cultivar ccri040029. to study the male sterile mechanisms of ccri9106, histological and itraq-facilitated proteomic analyses of anthers were performed. this data article contains data related to the research article titled itraq-facilitated proteomic profiling of ...201526958592
physiological performance and differential expression profiling of genes associated with drought tolerance in contrasting varieties of two gossypium species.cotton is mostly cultivated under rain-fed conditions in india, thus faces frequent drought conditions during its life cycle. drought being a major stress factor responsible for yield penalty, there has always been a high priority to generate knowledge on adaptation and tolerance of cotton. in the present study, four cotton varieties, jkc-770 and kc-2 (gossypium hirsutum), and jkc-717 and rahs-187(gossypium herbaceum), were imposed to drought. under drought condition, differential changes in phy ...201525149149
partial dominance, overdominance, epistasis and qtl by environment interactions contribute to heterosis in two upland cotton hybrids.based on two recombinant inbred line (ril) populations, two corresponding backcross (bc) populations were constructed to elucidate the genetic basis of heterosis in upland cotton (gossypium hirsutum l.). the yield, and yield components, of these populations were evaluated in three environments. at the single-locus level, 78 and 66 quantitative trait loci (qtl) were detected using composite interval mapping in ril and bc populations, respectively, and 29 qtl were identified based on mid-parental ...201526715091
atrav1 and atrav2 overexpression in cotton increases fiber length differentially under drought stress and delays flowering.there is a longstanding problem of an inverse relationship between cotton fiber qualities versus high yields. to better understand drought stress signaling and adaptation in cotton (gossypium hirsutum) fiber development, we expressed the arabidopsis transcription factors related_to_aba-insensitive3/viviparous1/(rav1) and atrav2, which encode apetala2-basic3 domain proteins shown to repress transcription of flowering_locus_t (ft) and to promote stomatal opening cell-autonomously. in three years o ...201526706061
polyamine and its metabolite h2o2 play a key role in the conversion of embryogenic callus into somatic embryos in upland cotton (gossypium hirsutum l.).the objective of this study was to increase understanding about the mechanism by which polyamines (pas) promote the conversion of embryogenic calli (ec) into somatic embryos in cotton (gossypium hirsutum l.). we measured the levels of endogenous pas and h2o2, quantified the expression levels of genes involved in the pas pathway at various stages of cotton somatic embryogenesis (se), and investigated the effects of exogenous pas and h2o2 on differentiation and development of ec. putrescine (put), ...201526697030
delineating the glycoproteome of elongating cotton fiber cells.the data presented here delineates the glycoproteome component in the elongating cotton fiber cells attained using complementary proteomic approaches followed by protein and n-linked glycosylation site identification (kumar et al., 2013) [1]. utilizing species specific protein sequence databases in proteomic approaches often leads to additional information that may not be obtained using cross-species databases. in this context we have reanalyzed our glycoproteome dataset with the gossypium arbor ...201526693171
microarray-based large scale detection of single feature polymorphism in gossypium hirsutum l.microarrays offer an opportunity to explore the functional sequence polymorphism among different cultivars of many crop plants. the affymetrix microarray expression data of five genotypes of gossypium hirsutum l. at six different fibre developmental stages was used to identify single feature polymorphisms (sfps). the background corrected and quantile-normalized log2 intensity values of all probes of triplicate data of each cotton variety were subjected to sfps call by using sam procedure in r la ...201526690522
partial dominance, overdominance and epistasis as the genetic basis of heterosis in upland cotton (gossypium hirsutum l.).determination of genetic basis of heterosis may promote hybrid production in upland cotton (gossypium hirsutum l.). this study was designed to explore the genetic mechanism of heterosis for yield and yield components in f2: 3 and f2: 4 populations derived from a hybrid 'xinza no. 1'. replicated yield field trials of the progenies were conducted in 2008 and 2009. phenotypic data analyses indicated overdominance in f1 for yield and yield components. additive and dominance effects at single-locus l ...201526618635
dynamic transcriptome analysis and volatile profiling of gossypium hirsutum in response to the cotton bollworm helicoverpa armigera.in response to insect herbivory, plants emit elevated levels of volatile organic compounds for direct and indirect resistance. however, little is known about the molecular and genomic basis of defense response that insect herbivory trigger in cotton plants and how defense mechanisms are orchestrated in the context of other biological processes. here we monitored the transcriptome changes and volatile characteristics of cotton plants in response to cotton bollworm (cbw; helicoverpa armigera) larv ...201526148847
promoting flowering, lateral shoot outgrowth, leaf development, and flower abscission in tobacco plants overexpressing cotton flowering locus t (ft)-like gene ghft1.flowering locus t (ft) encodes a mobile signal protein, recognized as major component of florigen, which has a central position in regulating flowering, and also plays important roles in various physiological aspects. a mode is recently emerging for the balance of indeterminate and determinate growth, which is controlled by the ratio of ft-like and terminal flower 1 (tfl1)-like gene activities, and has a strong influence on the floral transition and plant architecture. orthologs of ghft1 was pre ...201526136765
predawn respiration rates during flowering are highly predictive of yield response in gossypium hirsutum when yield variability is water-induced.respiratory carbon evolution by leaves under abiotic stress is implicated as a major limitation to crop productivity; however, respiration rates of fully expanded leaves are positively associated with plant growth rates. given the substantial sensitivity of plant growth to drought, it was hypothesized that predawn respiration rates (rpd) would be (1) more sensitive to drought than photosynthetic processes and (2) highly predictive of water-induced yield variability in gossypium hirsutum. two stu ...201526125121
analysis of upland cotton (gossypium hirsutum) response to verticillium dahliae inoculation by transcriptome sequencing.verticillium wilt is one of the main diseases in cotton (gossypium hirsutum), severely reduces yield and fiber quality, and is difficult to be con-trolled effectively. at present, the molecular mechanism that confers resistance to this disease is unclear. transcriptome sequencing is an important method to detect resistance genes, explore metabolic pathways, and study resistance mechanisms. in this study, the transcriptome of a disease-resistant inbred cot-ton line inoculated with verticillium da ...201526535625
microrna and mrna expression profiling analysis revealed the regulation of plant height in gossypium hirsutum.dwarf cottons are more resistant to damage from wind and rain and associated with stable, increased yields, and also desirable source for breeding the machine harvest varieties. in an effort to uncover the transcripts and mirna networks involved in plant height, the transcriptome and small rna sequencing were performed based on dwarf mutant ari1327 (a1), tall-culm mutant ari3697 (a3) and wild type ari971 (a9) in gossypium hirsutum.201526517985
a bil population derived from g. hirsutum and g. barbadense provides a resource for cotton genetics and breeding.to provide a resource for cotton genetics and breeding, an interspecific hybridization between gossypium hirsutum cv. emian22 and g. barbadense acc. 3-79 was made. a population of 54 bils (backcross inbred lines, bc1f8) was developed with the aim of transferring g. barbadense genes into g. hirsutum in order to genetically analyze these genes' function in a g. hirsutum background and create new germplasms for breeding. preliminary investigation of the morphological traits showed that the bils had ...201526517274
characterization and development of chloroplast microsatellite markers for gossypium hirsutum, and cross-species amplification in other gossypium species.cotton is an important economic crop worldwide; its fiber, commonly known as cotton lint, is the main natural source for the textile industry. sixty chloroplast microsatellites were identified and characterized from the complete sequence of the gossypium hirsutum chloroplast genome using a bioinformatic approach. twenty chloroplast microsatellite loci were polymorphic in the 66 gossypium germplasm accessions. a total of 85 alleles were detected, with allele numbers varying from 2-7 per locus. po ...201526505340
evaluating pilose, a cultigen of gossypium hirsutum, as a source of resistance to cotton fleahopper (hemiptera: miridae).cotton fleahopper (pseudatomoscelis seriatus reuter) (hemiptera: miridae) is a piercing-sucking insect that has emerged as a major pest of cotton (gossypium hirsutum l.) in texas. cotton fleahoppers feed on floral buds, commonly referred to as squares, causing damage and abscission, and subsequent yield loss. previous studies indicate that plant resistance to cotton fleahopper is present in upland cotton, but the mechanism of resistance remains undetermined. in this study, pilose, a cultigen of ...201526470352
in silico study for diversing the molecular pathway of pigment formation: an alternative to manual coloring in cotton fibers.diversity of colors in flowers and fruits is largely due to anthocyanin pigments. the flavonoid/anthocyanin pathway has been most extensively studied. dihydroflavonol 4-reductase (dfr) is a vital enzyme of the flavonoid pathway which displays major impact on the formation of anthocyanins, flavan 3-ols and flavonols. the substrate specificity of the dfr was found to play a crucial role in determination of type of anthocyanidins. altering the flavonoid/anthocyanin pathway through genetic engineeri ...201526442064
photosynthetic characteristics of the subtending leaf of cotton boll at different fruiting branch nodes and their relationships with lint yield and fiber quality.to investigate photosynthetic characteristics of the subtending leaf at the 2-3rd and 10-11th fruiting branch (fbn, fb2-3, and fb10-11), and their relationship with cotton yield and quality, field experiments were conducted using two cotton cultivars, kemian 1 and sumian 15. the results showed that with fbn increasing, chlorophyll (chl) components, pn and non-photochemical quenching (npq) in the subtending leaf significantly declined, while soluble sugar, amino acid and their ratio (c ss/c aa) a ...201526442060
identification of early salt stress responsive proteins in seedling roots of upland cotton (gossypium hirsutum l.) employing itraq-based proteomic technique.soil salinity is a major abiotic stress that limits plant growth and agricultural productivity. upland cotton (gossypium hirsutum l.) is highly tolerant to salinity; however, large-scale proteomic data of cotton in response to salt stress are still scant. here, an isobaric tag for relative and absolute quantitation (itraq)-based proteomic technique was employed to identify the early differentially expressed proteins (deps) from salt-treated cotton roots. one hundred and twenty-eight deps were id ...201526442045
cadmium (cd) localization in tissues of cotton (gossypium hirsutum l.), and its phytoremediation potential for cd-contaminated soils.phytoremediation using economically valuable, large biomass, non-edible plants is a promising method for metal-contaminated soils. this study investigated cotton's tolerance for cd and remediation potential through analyzing cd bioaccumulation and localization in plant organs under different soil cd levels. results showed cotton presents good tolerance when soil cd concentration ≤20.26 mg kg(-1). cotton had good cd accumulation ability under low soil cd levels (<1.26 mg kg(-1)), with a tf value ...201526419249
herbicide-resistant cotton (gossypium hirsutum) plants: an alternative way of manual weed removal.cotton yield has been badly affected by different insects and weed competition. in past application of multiple chemicals is required to manage insects and weed control was achieved by different conventional means, such as hand weeding, crop rotation and polyculture, because no synthetic chemicals were available. the control methods shifted towards high input and target-oriented methods after the discovery of synthetic herbicide in the 1930s. to utilise the transgenic approach, cotton plants exp ...201526383095
overexpression of a cotton (gossypium hirsutum) wrky gene, ghwrky34, in arabidopsis enhances salt-tolerance of the transgenic plants.soil salinity is one of the most serious threats in world agriculture, and often influences cotton growth and development, resulting in a significant loss in cotton crop yield. wrky transcription factors are involved in plant response to high salinity stress, but little is known about the role of wrky transcription factors in cotton so far. in this study, a member (ghwrky34) of cotton wrky family was functionally characterized. this protein containing a wrky domain and a zinc-finger motif belong ...201526332661
comparisons of lygus lineolaris (hemiptera: miridae) populations from two distinct geographical regions of mississippi.the tarnished plant bug, lygus lineolaris (palisot de beauvois) (hemiptera: miridae), is a major pest of cotton (gossypium hirsutum l.) in the state of mississippi. economic data indicate that l. lineolaris is a more serious pest of cotton in the delta region of mississippi than in the hills region; however, little data exist comparing the two populations. two experiments were undertaken to compare l. lineolaris from these two geographically distinct regions. in the first experiment, colonies of ...201526313997
influence of crop management and environmental factors on wolf spider assemblages (araneae: lycosidae) in an australian cotton cropping system.wolf spiders (lycosidae) are the most abundant ground-hunting spiders in the australian cotton (gossypium hirsutum l.) agroecosystems. these spiders have potential in controlling pest bollworms, helicoverpa spp. (lepidoptera: noctuidae) in minimum-tilled fields. a study was carried out during a wet growing season (2011-2012) in narrabri, new south wales, australia, to determine how different crop rotations and tillage affect wolf spider assemblages in cotton fields. spider abundance and species ...201526308820
a high-density ssr genetic map constructed from a f2 population of gossypium hirsutum and gossypium darwinii.the cultivated allotetraploid species gossypium hirsutum, accounts for 90% of the world cotton production, has narrow genetic basis that's why its yield, quality or stress resistance breeding is stagnant. it is therefore, essential to explore desirable genes from gossypium darwinii which has enviable traits such as high fiber fineness, drought tolerance, fusarium and verticillium resistance. we used g. darwinii as primary plant materials in this study not only to enrich the genetic diversity of ...201526275937
high resolution consensus mapping of quantitative trait loci for fiber strength, length and micronaire on chromosome 25 of the upland cotton (gossypium hirsutum l.).cotton (gossypium hirsutum l.) is an important agricultural crop that provides renewable natural fiber resources for the global textile industry. technological developments in the textile industry and improvements in human living standards have increased the requirement for supplies and better quality cotton. upland cotton 0-153 is an elite cultivar harboring strong fiber strength genes. to conduct quantitative trait locus (qtl) mapping for fiber quality in 0-153, we developed a population of 19 ...201526262992
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