Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
larval dispersal of spodoptera frugiperda strains on bt cotton: a model for understanding resistance evolution and consequences for its management.high dispersal of lepidoptera larvae between non-bt and bt cotton plants can favour the evolution of insect resistance; however, information on host acceptance of neonates in tropical transgenic crops is scarce. therefore, the purposes of this study were as follows: (i) to investigate the feeding behaviour of susceptible and cry1f-resistant strains of spodoptera frugiperda (j.e. smith) on bt and non-bt cotton (gossypium hirsutum l.) varieties and (ii) to understand the possible effects of cotton ...201729170392
ghjaz2 attenuates cotton resistance to biotic stresses via inhibiting the transcriptional activity of ghbhlh171.plants evolve effective mechanisms to protect themselves against multiple stresses and employ jasmonates (ja) as vital defense signals to defend against pathogen infection. accumulation of ja induced by signals from biotic and abiotic stresses, results in degradation of jasmonate-zim-domain (jaz) proteins, and then de-repressed the jaz-repressed transcription factors (such as myc2) to activate defense responses and developmental processes. here, we characterized a jaz family protein, ghjaz2, fro ...201728665036
a new species of scopulariopsis and its synergistic effect on pathogenicity of verticillium dahliae on cotton plants.a new species, scopulariopsis gossypii, was found to be present in the vascular bundles of cotton plants (gossypium hirsutum) infected by verticillium dahliae which is an economically important pathogen in hangzhou, china. the fungus was only present in the diseased plants, but it never became isolated from the healthy plants. scanning electron microscopy showed that the fungus was present in parenchyma cells enclosing vessels in dark brown vascular tissues of stems, and produced asexual conidia ...201728602397
the variation in the rhizosphere microbiome of cotton with soil type, genotype and developmental stage.plant roots and soil microorganisms interact with each other mainly in the rhizosphere. changes in the community structure of the rhizosphere microbiome are influenced by many factors. in this study, we determined the community structure of rhizosphere bacteria in cotton, and studied the variation of rhizosphere bacterial community structure in different soil types and developmental stages using tm-1, an upland cotton cultivar (gossypium hirsutum l.) and hai 7124, a sea island cotton cultivar (g ...201728638057
assessment of gene flow between gossypium hirsutum and g. herbaceum: evidence of unreduced gametes in the diploid progenitor.in the framework of a gene flow assessment, we investigated the natural hybridization rate between gossypium hirsutum (aadd genome) and g. herbaceum (aa genome). the latter species, a diploid progenitor of g. hirsutum, is spontaneously present in south africa. reciprocal crosses were performed without emasculation between g. herbaceum and g. hirsutum neither examination of the morphological characteristics nor flow cytometry analysis of the 335 plants resulting from the g. hirsutum × g. herbaceu ...201728546386
evolution, gene expression profiling and 3d modeling of csld proteins in cotton.among cesa-like gene superfamily, the cellulose synthase-like d (csld) genes are most similar to cellulose synthase genes and have been reported to be involved in tip-growing cell and stem development. however, there has been no genome-wide characterization of this gene subfamily in cotton. we thus sought to analyze the evolution and functional characterization of csld proteins in cotton based on fully sequenced cotton genomes.201728693426
genome-wide analysis of wox genes in upland cotton and their expression pattern under different stresses.wuschel-related homeobox (wox) family members play significant roles in plant growth and development, such as in embryo patterning, stem-cell maintenance, and lateral organ formation. the recently published cotton genome sequences allow us to perform comprehensive genome-wide analysis and characterization of wox genes in cotton.201728683794
the genome of the cotton bacterial blight pathogen xanthomonas citri pv. malvacearum strain msct1.xanthomonas citri pv. malvacearum is a major pathogen of cotton, gossypium hirsutum l.. in this study we report the complete genome of the x. citri pv. malvacearum strain msct1 assembled from long read dna sequencing technology. the msct1 genome is the first x. citri pv. malvacearum genome with complete coding regions for x. citri pv. malvacearum transcriptional activator-like effectors. in addition functional and structural annotations are presented in this study that will provide a foundation ...201728770027
the jasmonate zim-domain gene family mediates ja signaling and stress response in cotton.jasmonate zim-domain (jaz) family proteins are involved in regulating diverse biological processes in plants. however, their functions have not been well characterized in cotton (gossypium spp.). in the present study, 13, 15, 25 and 30 jaz genes were identified in gossypium arboretum, gossypium raimondii, gossypium barbadense and gossypium hirsutum, respectively, based on gene homology. selection and variation analyses showed that the single nucleotide polymorphism (snp) density of ghjaz genes i ...201729036515
genetic and phenotypic effects of chromosome segments introgressed from gossypium barbadense into gossypium hirsutum.mbi9915 is an introgression cotton line with excellent fiber quality. it was obtained by advanced backcrossing and continuous inbreeding from an interspecific cross between the upland cotton (gossypium hirsutum) cultivar ccri36 as the recurrent parent and the sea island cotton (g. barbadense) cultivar hai1, as the donor parent. to study the genetic effects of the introgressed chromosome segments in g. hirsutum, an f2 secondary segregating population of 1537 individuals was created by crossing mb ...201728931074
large-scale identification of gossypium hirsutum genes associated with verticillium dahliae by comparative transcriptomic and reverse genetics analysis.verticillium wilt is a devastating disease of cotton, which is caused by the soil-borne fungus verticillium dahliae (v. dahliae). although previous studies have identified some genes or biological processes involved in the interaction between cotton and v. dahliae, its underlying molecular mechanism remains unclear, especially in g. hirsutum. in the present study, we obtained an overview of transcriptome characteristics of resistant upland cotton (g. hirsutum) after v. dahliae infection at 24 h ...201728767675
qtl analysis of transgressive nematode resistance in tetraploid cotton reveals complex interactions in chromosome 11 regions.transgressive segregation in cotton (gossypium spp.) provides an important approach to enhance resistance to the major pest root-knot nematode (rkn) meloidogyne incognita. our previous studies reported transgressive rkn resistance in an intraspecific gossypium hirsutum resistant nemx × susceptible sj-2 recombinant inbred line (ril) population and early generations of interspecific cross gossypium barbadense (susceptible pima s-7) × g. hirsutum (nemx). however, the underlying functional mechanism ...201729209344
expression of pathogenesis-related genes in cotton roots in response to verticillium dahliae pamp molecules.verticillium wilt disease becomes a major threat to many economically important crops. it is unclear whether and how plant immunity takes place during cotton-verticillium interaction due to the lack of marker genes. taking advantage of cotton (gossypium hirsutum) genome, we discovered pathogenesis-related (pr) gene families, which have been widely used as markers of immune responses in plants. to profile the expression of g. hirsutum pr genes in the process of plant immunity, we treated cotton r ...201728755291
5-aminolevulinic acid dehydratase gene dosage affects programmed cell death and immunity.programmed cell death (pcd) is an important form to protect plants from pathogen attack. however, plants must precisely control the pcd process under microbe attacks to avoid detrimental effects. the complexity of how plants balance the defense activation and pcd requires further clarification. lesion mimic mutants constitute an excellent material to study the crosstalk between them. here, we identified a gossypium hirsutum (cotton) lesion mimic mutant (ghlmm), which exhibits necrotic leaf damag ...201728751313
long noncoding rnas involve in resistance to verticillium dahliae, a fungal disease in cotton.long noncoding rnas (lncrnas) have several known functions in plant development, but their possible roles in responding to plant disease remain largely unresolved. in this study, we described a comprehensive disease-responding lncrna profiles in defence against a cotton fungal disease verticillium dahliae. we further revealed the conserved and specific characters of disease-responding process between two cotton species. conservatively for two cotton species, we found the expression dominance of ...201729149461
comparative transcriptome analysis of cotton fiber development of upland cotton (gossypium hirsutum) and chromosome segment substitution lines from g. hirsutum × g. barbadense.how to develop new cotton varieties possessing high yield traits of upland cotton and superior fiber quality traits of sea island cotton remains a key task for cotton breeders and researchers. while multiple attempts bring in little significant progresses, the development of chromosome segment substitution lines (cssls) from gossypium barbadense in g. hirsutum background provided ideal materials for aforementioned breeding purposes in upland cotton improvement. based on the excellent fiber perfo ...201728886694
genome-wide identification of cbl family and expression analysis of cbls in response to potassium deficiency in cotton.calcineurin b-like (cbl) proteins, as calcium sensors, play pivotal roles in plant responses to diverse abiotic stresses and in growth and development through interaction with cbl-interacting protein kinases (cipks). however, knowledge about functions and evolution of cbls in gossypium plants is scarce. here, we conducted a genome-wide survey and identified 13, 13 and 22 cbl genes in the progenitor diploid gossypium arboreum and gossypium raimondii, and the cultivated allotetraploid gossypium hi ...201728828254
tobacco rattle virus-based silencing of enoyl-coa reductase gene and its role in resistance against cotton wilt disease.a tobacco rattle virus (trv)-based virus-induced gene silencing assay was employed as a reverse genetic approach to study gene function in cotton (gossypium hirsutum). this approach was used to investigate the function of the enoyl-coa reductase (ghecr) gene in pathogen defense. amino acid sequence alignment of arabidopsis ecr with homologous sequence from g. hirsutum, g. arboreum, g. herbaceum and g. barbadense showed that ecrs are highly conserved among these species. trv-based silencing of gh ...201728567686
detoxification of fusaric acid by the soil microbe mucor rouxii.fusarium oxysporum f. sp. vasinfectum race 4 (vcg0114), which causes root rot and wilt of cotton (gossypium hirsutum and g. barbadense), has been identified recently for the first time in the western hemisphere in certain fields in the san joaquin valley of california. this pathotype produces copious quantities of the plant toxin fusaric acid (5-butyl-2-pyridinecarboxylic acid) compared to other isolates of f. oxysporum f. sp. vasinfectum (fov) that are indigenous to the united states. fusaric a ...201728538103
transcriptome analysis suggests that chromosome introgression fragments from sea island cotton (gossypium barbadense) increase fiber strength in upland cotton (gossypium hirsutum).as high-strength cotton fibers are critical components of high quality cotton, developing cotton cultivars with high-strength fibers as well as high yield is a top priority for cotton development. recently, chromosome segment substitution lines (cssls) have been developed from high-yield upland cotton (gossypium hirsutum) crossed with high-quality sea island cotton (g. barbadense). here, we constructed a cssl population by crossing ccri45, a high-yield upland cotton cultivar, with hai1, a sea is ...201728874383
transcriptomics reveals multiple resistance mechanisms against cotton leaf curl disease in a naturally immune cotton species, gossypium arboreum.cotton leaf curl disease (clcud), caused by cotton leaf curl viruses (clcuvs), is among the most devastating diseases in cotton. while the widely cultivated cotton species gossypium hirsutum is generally susceptible, the diploid species g. arboreum is a natural source for resistance against clcud. however, the influence of clcud on the g. arboreum transcriptome and the interaction of clcud with g. arboreum remains to be elucidated. here we have used an rna-seq based study to analyze differential ...201729162860
suppression of cotton leaf curl disease symptoms in gossypium hirsutum through over expression of host-encoded mirnas.cotton leaf curl disease (clcud), a major factor resulting in the enormous yield losses in cotton crop, is caused by a distinct monopartite begomovirus in association with cotton leaf curl multan betasatellite (clcumb). micro(mi)rnas are known to regulate gene expression in eukaryotes, including antiviral defense in plants. in a previous study, we had computationally identified a set of cotton mirnas, which were shown to have potential targets in the genomes of cotton leaf curl multan virus (clc ...201729017848
characterization of conserved circular rna in polyploid gossypium species and their ancestors.circular rna (circrna) is a regulatory class of long, noncoding rna found in both plant and animal kingdoms. the profile and characterization of circrna in cotton species remains to be explored. here, using 24 rrna-depleted rna-seq libraries of putative diploid progenitors of gossypium spp., gossypium arboreum and gossypium raimondii, their interspecies hybrid (f1 ) and allotetraploid gossypium hirsutum, 1041, 1478, 1311, and 499 circrnas were identified in each cotton species, respectively. a p ...201728971489
a genome-scale analysis of the pin gene family reveals its functions in cotton fiber development.the pin-formed (pin) protein, the most important polar auxin transporter, plays a critical role in the distribution of auxin and controls multiple biological processes. however, characterizations and functions of this gene family have not been identified in cotton. here, we identified the pin family in gossypium hirsutum, gossypium arboreum, and gossypium raimondii. this gene family was divided into seven subgroups. a chromosomal distribution analysis showed that ghpin genes were evenly distribu ...201728424725
genome-wide comparative analysis of nbs-encoding genes in four gossypium species.nucleotide binding site (nbs) genes encode a large family of disease resistance (r) proteins in plants. the availability of genomic data of the two diploid cotton species, gossypium arboreum and gossypium raimondii, and the two allotetraploid cotton species, gossypium hirsutum (tm-1) and gossypium barbadense allow for a more comprehensive and systematic comparative study of nbs-encoding genes to elucidate the mechanisms of cotton disease resistance.201728403834
comparison of rhizosphere properties as affected by different bt- and non-bt-cotton (gossypium hirsutum l.) genotypes and fertilization.incorporation of genetically modified crops in the cropping system raises the need for studying the effect of these crops on the soil ecosystem. the current study aimed to compare the effect of bacillus thuringiensis (bt)- and non-bt-cotton (gossypium hirsutum l.) genotypes on rhizosphere properties under fertilized and unfertilized soil conditions. one non-bt-cotton (iub 75) and four bt-cotton varieties (iub-222, mm-58, iub-13, fh-142) were sown in a randomized complete block design (rcbd) in a ...201728527035
identification and characterization of the diverse stress-responsive r2r3-rmyb transcription factor from hibiscus sabdariffa l.various regulatory proteins play a fundamental role to manage the healthy plant growth under stress conditions. differential display reverse transcriptase pcr and random amplification of cdna ends (race) was used to explore the osmotic stress-responsive transcripts. we identified and characterized the salt stress-responsive r2r3 type rmyb transcription factor from hibiscus sabdariffa which has an open reading frame of 690 bp, encoding 229 long chain amino acids. in silico analysis confirmed the ...201729181384
azotobacter chroococcum as a potentially useful bacterial biofertilizer for cotton (gossypium hirsutum): effect in reducing n fertilization.the aim of this research was to evaluate whether the application of two plant growth-promoting (rhizo)bacteria might reduce nitrogen fertilization doses in cotton. we used strains azotobacter chroococcum ac1 and ac10 for their proven ability to promote seed germination and cotton growth. these microorganisms were characterized by their plant growth-promoting activities. then, we conducted a glasshouse study to evaluate the plant growth promoting ability of these strains with reduced doses of ure ...201728864227
flavobacterium gossypii sp. nov. isolated from the root tissue of field-grown cotton.a yellow-pigmented bacterial strain, jm-222t, isolated from the root tissue of field-grown cotton (gossypium hirsutum) in auburn, alabama, usa, was studied for its taxonomic allocation. cells of the isolate were rod-shaped and gram-stain-negative. a blast analysis using the eztaxon database showed that strain jm-222t shared highest 16s rrna gene sequence similarity with the type strain of flavobacterium endophyticum (98.9 %) followed by flavobacterium qiangtangense (96.7 %). 16s rrna gene sequen ...201728840797
a harpin elicitor induces the expression of a coiled-coil nucleotide binding leucine rich repeat (cc-nb-lrr) defense signaling gene and others functioning during defense to parasitic nematodes.the bacterial effector harpin induces the transcription of the arabidopsis thaliana non-race specific disease resistance 1/harpin induced1 (ndr1/hin1) coiled-coil nucleotide binding leucine rich repeat (cc-nb-lrr) defense signaling gene. in glycine max, gm-ndr1-1 transcripts have been detected within root cells undergoing a natural resistant reaction to parasitism by the syncytium-forming nematode heterodera glycines, functioning in the defense response. expressing gm-ndr1-1 in gossypium hirsutu ...201729107936
abp9, a maize bzip transcription factor, enhances tolerance to salt and drought in transgenic cotton.abp9 , encoding a bzip transcription factor from maize, enhances tolerance to multiple stresses and may participate in the aba signaling pathway in transgenic cotton by altering physiological and biochemical processes and stress-related gene expression. abiotic stresses, such as soil salinity and drought, negatively affect growth, development, and yield in cotton. gene abp9, which encodes a bzip transcription factor, binds to the abscisic acid (aba)-responsive-element (abre2) motif of the maize ...201728474114
micrornas as reference genes for quantitative pcr in cotton.cotton (gossypium hirsutum) is the most important non-food plant in the world. studies concerning the fiber quality and plant fitness of cotton at molecular level depend on high sensitive and reproducible gene-expression assays. however, only a few reports have described genes suitable for normalizing gene expression data. in this study, we report for the first time that micrornas (mirnas) are reliable reference genes (rgs) for cotton gene expression normalization in quantitative real-time rever ...201728414734
transgenic cotton expressing cry10aa toxin confers high resistance to the cotton boll weevil.genetically modified (gm) cotton plants that effectively control cotton boll weevil (cbw), which is the most destructive cotton insect pest in south america, are reported here for the first time. this work presents the successful development of a new gm cotton with high resistance to cbw conferred by cry10aa toxin, a protein encoded by entomopathogenic bacillus thuringiensis (bt) gene. the plant transformation vector harbouring cry10aa gene driven by the cotton ubiquitination-related promoter uc ...201728081289
a glycine max homolog of non-race specific disease resistance 1 (ndr1) alters defense gene expression while functioning during a resistance response to different root pathogens in different genetic backgrounds.a glycine max homolog of the arabidopsis thaliana non-race specific disease resistance 1 (ndr1) coiled-coil nucleotide binding leucine rich repeat (cc-nb-lrr) defense signaling gene (gm-ndr1-1) is expressed in root cells undergoing a defense response to the root pathogenic nematode, heterodera glycines. gm-ndr1-1 overexpression in the h. glycines-susceptible genotype g. max[williams 82/pi 518671] impairs parasitism. in contrast, gm-ndr1-1 rna interference (rnai) in the h. glycines-resistant geno ...201728273511
application of bacteria from non-cultivated plants to promote growth, alter root architecture and alleviate salt stress of cotton.cotton seeds are frequently treated with acid to remove fibres and reduce seed-transmitted diseases. this process also eliminates beneficial bacteria on the seed surface. the goal of this research was to seek and apply beneficial bacteria to acid delinted cotton seeds to evaluate their growth-promoting and salt stress alleviating effects in seedlings.201728176460
ghmyb1 regulates scw stage-specific expression of the ghgdsl promoter in the fibres of gossypium hirsutum l.secondary cell wall (scw) biosynthesis is an important stage of the cotton fibre development, and its transcriptional regulation is poorly understood. we selected the gossypium hirsutum gdsl (ghgdsl) lipase/hydrolase gene (cotad_74480), which is expressed during scw biosynthesis (19 through to 25 days postanthesis; dpa), for study. t1 -transgenic cotton lines expressing the β-glucuronidase (gus) reporter under the control of a 1026-bp promoter fragment of ghgdsl (pghgdsl ) showed 19 dpa stage-sp ...201728182326
overexpression of cotton pyl genes in arabidopsis enhances the transgenic plant tolerance to drought stress.pyr/pyl/rcar proteins are putative abscisic acid (aba) receptors that play important roles in plant responses to biotic and abiotic stresses. in this study, 27 predicted pyl proteins were identified in cotton (gossypium hirsutum). sequence analysis showed they are conserved in structures. phylogenetic analysis showed that cotton pyl family could be categorized into three groups. yeast two-hybrid assay revealed that the ghpyl proteins selectively interacted with some ghpp2c proteins. quantitative ...201728388505
the glutathione peroxidase gene family in gossypium hirsutum: genome-wide identification, classification, gene expression and functional analysis.the plant glutathione peroxidase (gpx) family consists of multiple isoenzymes with distinct subcellular locations, tissue-specific expression patterns and environmental stress responses. in this study, 13 putative gpxs from the genome of gossypium hirsutum (ghgpxs) were identified and a conserved pattern among plant gpxs were exhibited, besides this they also responded to multiple environmental stresses and we predicted that they had hormone responsive cis-elements in their promoter regions. mos ...201728300195
identification of a new cotton disease caused by an atypical cotton leafroll dwarf virus in argentina.an outbreak of a new disease occurred in cotton (gossypium hirsutum) fields in northwest argentina starting in the 2009-10 growing season and is still spreading steadily. the characteristic symptoms of the disease included slight leaf rolling and a bushy phenotype in the upper part of the plant. in this study, we determined the complete nucleotide sequences of two independent virus genomes isolated from cotton blue disease (cbd)-resistant and -susceptible cotton varieties. this virus genome comp ...201728035870
functional characterization of agamous-subfamily members from cotton during reproductive development and in response to plant hormones.expression analysis of the ag -subfamily members from g. hirsutum during flower and fruit development. reproductive development in cotton, including the fruit and fiber formation, is a complex process; it involves the coordinated action of gene expression regulators, and it is highly influenced by plant hormones. several studies have reported the identification and expression of the transcription factor family mads-box members in cotton ovules and fibers; however, their roles are still elusive d ...201728176007
development and survival of spodoptera exigua (lepidoptera: noctuidae) on alternate crops in cotton cropping pattern, with implications to integrated pest management.spodoptera exigua (hübner) is a polyphagous pest that shifts its population to different hosts during its life cycle to receive nutritive advantages. therefore, demographic evaluation of alternate hosts is important for effective pest management. here, we have evaluated castor (ricinus communis l.), cauliflower (brassica oleracea l.), cotton (gossypium hirsutum l.), okra (abelmoschus esculentus l.), and spinach (spinacia oleracea l.) for growth, survival, and population development of s. exigua. ...201728383691
the verticillium-specific protein vdscp7 localizes to the plant nucleus and modulates immunity to fungal infections.fungal pathogens secrete effector proteins to suppress plant basal defense for successful colonization. resistant plants, however, can recognize effectors by cognate r proteins to induce effector-triggered immunity (eti). by analyzing secretomes of the vascular fungal pathogen verticillium dahliae, we identified a novel secreted protein vdscp7 that targets the plant nucleus. the green fluorescent protein (gfp)-tagged vdscp7 gene with either a mutated nuclear localization signal motif or with add ...201728407259
comparative host selection responses of specialist (helicoverpa assulta) and generalist (helicoverpa armigera) moths in complex plant environments.we tested the behavioral responses of ovipositing females and natal larvae of two sibling species, a generalist helicoverpa armigera (hübner) and a specialist helicoverpa assulta (guenée), to odor sources emitted from different combinations of six plant species (tobacco, nicotiana tabacum; hot pepper, capsicum annuum; tomato, solanum esculentum; cotton, gossypium hirsutum; peanut, arachis hypogaea; maize, zea mays). under the conditions of plant materials versus corresponding controls, both stag ...201728182679
responses of neonicotinoid-resistant and susceptible frankliniella fusca life stages to multiple insecticide groups in cotton.detection of neonicotinoid resistance in populations of tobacco thrips, frankliniella fusca hinds, throughout the southeastern us has motivated an examination of alternative insecticides to control problematic infestations on seedling cotton. the objective of this study was to refine understanding of stage-specific mortality and reduced oviposition of several common insecticides (acephate, abamectin, cyantraniliprole, spinetoram, imidacloprid, imidacloprid + thiodicarb, thiamethoxam) on neonicot ...201728401732
cotton defense induction patterns under spatially, temporally and quantitatively varying herbivory levels.in its defense against herbivores, cotton (gossypium sp.) relies in part on the production of a set of inducible, non-volatile terpenoids. under uniform damage levels, in planta allocation of induced cotton terpenoids has been found to be highest in youngest leaves, supporting assumptions of the optimal defense theory (odt) which predicts that plants allocate defense compounds to tissues depending on their value and the likelihood of herbivore attack. however, our knowledge is limited on how var ...201728270830
genomic insights into divergence and dual domestication of cultivated allotetraploid cottons.cotton has been cultivated and used to make fabrics for at least 7000 years. two allotetraploid species of great commercial importance, gossypium hirsutum and gossypium barbadense, were domesticated after polyploidization and are cultivated worldwide. although the overall genetic diversity between these two cultivated species has been studied with limited accessions, their population structure and genetic variations remain largely unknown.201728219438
genome-wide identification of the tify gene family in three cultivated gossypium species and the expression of jaz genes.tify proteins are plant-specific proteins containing tify, jaz, ppd and zml subfamilies. a total of 50, 54 and 28 members of the tify gene family in three cultivated cotton species-gossypium hirsutum, gossypium barbadense and gossypium arboretum-were identified, respectively. the results of phylogenetic analysis showed that these tify genes were divided into eight clusters. the different clusters of gene family members often have similar gene structures, including the number of exons. the result ...201728186193
sequential sampling plan of anthonomus grandis (coleoptera: curculionidae) in cotton plants.the boll weevil, anthonomus grandis grandis boheman (coleoptera: curculionidae), is one of the most important pests of cotton production worldwide. the objective of this work was to develop a sequential sampling plan for the boll weevil. the studies were conducted in maracaju, ms, brazil, in two seasons with cotton cultivar fm 993. a 10,000-m2 area of cotton was subdivided into 100 of 10- by 10-m plots, and five plants per plot were evaluated weekly, recording the number of squares with feeding  ...201728334212
genome-wide divergence, haplotype distribution and population demographic histories for gossypium hirsutum and gossypium barbadense as revealed by genome-anchored snps.use of 10,129 singleton snps of known genomic location in tetraploid cotton provided unique opportunities to characterize genome-wide diversity among 440 gossypium hirsutum and 219 g. barbadense cultivars and landrace accessions of widespread origin. using the snps distributed genome-wide, we examined genetic diversity, haplotype distribution and linkage disequilibrium patterns in the g. hirsutum and g. barbadense genomes to clarify population demographic history. diversity and identity-by-state ...201728128280
fine mapping and candidate gene analysis of qfl-chr1, a fiber length qtl in cotton.a fiber length qtl, qfl-chr1, was fine mapped to a 0.9 cm interval of cotton chromosome 1. two positional candidate genes showed positive correlation between gene expression level and fiber length. prior analysis of a backcross-self mapping population derived from a cross between gossypium hirsutum l. and g. barbadense l. revealed a qtl on chromosome 1 associated with increased fiber length (qfl-chr1), which was confirmed in three independent populations of near-isogenic introgression lines (nii ...201728361363
developmental features of cotton fibre middle lamellae in relation to cell adhesion and cell detachment in cultivars with distinct fibre qualities.cotton fibre quality traits such as fibre length, strength, and degree of maturation are determined by genotype and environment during the sequential phases of cotton fibre development (cell elongation, transition to secondary cell wall construction and cellulose deposition). the cotton fibre middle lamella (cfml) is crucial for both cell adhesion and detachment processes occurring during fibre development. to explore the relationship between fibre quality and the pace at which cotton fibres dev ...201728359260
genome-wide identification of membrane-bound fatty acid desaturase genes in gossypium hirsutum and their expressions during abiotic stress.membrane-bound fatty acid desaturases (fads) are of great importance and play multiple roles in plant growth and development. in the present study, 39 full-length fad genes, based on database searches, were identified in tetraploid upland cotton (gossypium hirsutum l.) and were phylogenetically clustered into four subfamilies. genomic localization revealed that 34 genes were mapped on 22 chromosomes, and five genes were positioned on the scaffold sequences. the fad genes of g. hirsutum in the sa ...201728374822
genome-wide association study discovered candidate genes of verticillium wilt resistance in upland cotton (gossypium hirsutum l.).verticillium wilt (vw), caused by infection by verticillium dahliae, is considered one of the most yield-limiting diseases in cotton. to examine the genetic architecture of cotton vw resistance, we performed a genome-wide association study (gwas) using a panel of 299 accessions and 85,630 single-nucleotide polymorphisms (snps) detected using the specific-locus amplified fragment sequencing (slaf-seq) approach. trait-snp association analysis detected a total of 17 significant snps at p < 1.17 × 1 ...201728371164
qtl analysis of cotton fiber length in advanced backcross populations derived from a cross between gossypium hirsutum and g. mustelinum.qtls for fiber length mapped in three generations of advanced backcross populations derived from crossing gossypium hirsutum and gossypium mustelinum showed opportunities to improve elite cottons by introgression from wild relatives. the molecular basis of cotton fiber length in crosses between gossypium hirsutum and gossypium mustelinum was dissected using 21 bc3f2 and 12 corresponding bc3f2:3 and bc3f2:4 families. sixty-five quantitative trait loci (qtls) were detected by one-way analysis of v ...201728349176
microrna expression profiles during cotton (gossypium hirsutum l) fiber early development.the role of micrornas (mirnas) during cotton fiber development remains unclear. here, a total of 54 mirnas belonging to 39 families were selected to characterize mirna regulatory mechanism in eight different fiber development stages in upland cotton cv bm-1. among 54 mirnas, 18 mirnas were involved in cotton fiber initiation and eight mirnas were related to fiber elongation and secondary wall biosynthesis. additionally, 3,576 protein-coding genes were candidate target genes of these mirnas, whic ...201728327647
population structure and genetic basis of the agronomic traits of upland cotton in china revealed by a genome-wide association study using high-density snps.gossypium hirsutum l. represents the largest source of textile fibre, and china is one of the largest cotton-producing and cotton-consuming countries in the world. to investigate the genetic architecture of the agronomic traits of upland cotton in china, a diverse and nationwide population containing 503 g. hirsutum accessions was collected for a genome-wide association study (gwas) on 16 agronomic traits. the accessions were planted in four places from 2012 to 2013 for phenotyping. the cottonsn ...201728301713
targeted mutagenesis in cotton (gossypium hirsutum l.) using the crispr/cas9 system.the crispr (clustered regularly interspaced short palindromic repeats)/cas9 system has been widely used for genome editing in various plants because of its simplicity, high efficiency and design flexibility. however, to our knowledge, there is no report on the application of crispr/cas9-mediated targeted mutagenesis in cotton. here, we report the genome editing and targeted mutagenesis in upland cotton (gossypium hirsutum l., hereafter cotton) using the crispr/cas9 system. we designed two guide ...201728287154
asymmetric subgenome selection and cis-regulatory divergence during cotton domestication.comparative population genomics offers an excellent opportunity for unraveling the genetic history of crop domestication. upland cotton (gossypium hirsutum) has long been an important economic crop, but a genome-wide and evolutionary understanding of the effects of human selection is lacking. here, we describe a variation map for 352 wild and domesticated cotton accessions. we scanned 93 domestication sweeps occupying 74 mb of the a subgenome and 104 mb of the d subgenome, and identified 19 cand ...201728263319
genome-wide association study discovered genetic variation and candidate genes of fibre quality traits in gossypium hirsutum l.genetic improvement of fibre quality is one of the main breeding goals for the upland cotton, gossypium hirsutum, but there are difficulties with precise selection of traits. therefore, it is important to improve the understanding of the genetic basis of phenotypic variation. in this study, we conducted phenotyping and genetic variation analyses of 719 diverse accessions of upland cotton based on multiple environment tests and a recently developed cotton 63k illumina infinium snp array and perfo ...201728064470
optimizing the phosphorus use in cotton by using csm-cropgro-cotton model for semi-arid climate of vehari-punjab, pakistan.crop nutrient management is an essential component of any cropping system. with increasing concerns over environmental protection, improvement in fertilizer use efficiencies has become a prime goal in global agriculture system. phosphorus (p) is one of the most important nutrients, and strategies are required to optimize its use in important arable crops like cotton (gossypium hirsutum l.) that has great significance. sustainable p use in crop production could significantly avoid environmental h ...201728054268
crispr/cas9-mediated targeted mutagenesis in upland cotton (gossypium hirsutum l.).the clustered, regularly interspaced, short palindromic repeats (crispr)/crispr associated (cas)9 protein system has emerged as a simple and efficient tool for genome editing in eukaryotic cells. it has been shown to be functional in several crop species, yet there are no reports on the application of this or any other genome editing technologies in the cotton plant. cotton is an important crop that is grown mainly for its fiber, but its seed also serves as a useful source of edible oil and feed ...201728258551
a high-efficiency crispr/cas9 system for targeted mutagenesis in cotton (gossypium hirsutum l.).the complex allotetraploid genome is one of major challenges in cotton for repressing gene expression. developing site-specific dna mutation is the long-term dream for cotton breeding scientists. the clustered regularly interspaced short palindromic repeats/crispr-associated protein 9 (crispr/cas9) system is emerging as a robust biotechnology for targeted-dna mutation. in this study, two sgrnas, ghmyb25-like-sgrna1 and ghmyb25-like-sgrna2, were designed in the identical genomic regions of ghmyb2 ...201728256588
identification of marker-trait associations for lint traits in cotton.harvesting high quality lint, a long-awaited breeding goal-accomplished partly, can be achieved by identifying dna markers which could be used for diagnosing cotton plants containing the desired traits. in the present studies, a total of 185 cotton genotypes exhibiting diversity for lint traits were selected from a set of 546 genotypes evaluated for fiber traits in 2009. these genotypes were extensively studied for three consecutive years (2011-2013) at three different locations. significant gen ...201728220132
diversity analysis of cotton (gossypium hirsutum l.) germplasm using the cottonsnp63k array.cotton germplasm resources contain beneficial alleles that can be exploited to develop germplasm adapted to emerging environmental and climate conditions. accessions and lines have traditionally been characterized based on phenotypes, but phenotypic profiles are limited by the cost, time, and space required to make visual observations and measurements. with advances in molecular genetic methods, genotypic profiles are increasingly able to identify differences among accessions due to the larger n ...201728158969
fine-mapping qfs07.1 controlling fiber strength in upland cotton (gossypium hirsutum l.).key message: qfs07.1 controlling fiber strength was fine-mapped to a 62.6-kb region containing four annotated genes. rt-qpcr and sequence of candidate genes identified an lrr rlk gene as the most likely candidate. fiber strength is an important component of cotton fiber quality and is associated with other properties, such as fiber maturity, fineness, and length. stable qtl qfs07.1, controlling fiber strength, had been identified on chromosome 7 in an upland cotton recombinant inbred line (ril) ...201728144698
dissecting genetic network of fruit branch traits in upland cotton by association mapping using ssr markers.genetic architecture of branch traits has large influences on the morphological structure, photosynthetic capacity, planting density, and yield of upland cotton (gossypium hirsutum l.). this research aims to reveal the genetic effects of six branch traits, including bottom fruit branch node number (bfbnn), bottom fruit branch length (bfbl), middle fruit branch node number (mfbnn), middle fruit branch length (mfbl), upper fruit branch node number (ufbnn), and upper fruit branch length (ufbl). ass ...201728121983
ectopic expression of two areb/abf orthologs increases drought tolerance in cotton (gossypium hirsutum).plants have evolved complex molecular, cellular and physiological mechanisms to respond to environmental stressors. because of the inherent complexity of this response, genetic manipulation to substantially improve water deficit tolerance, particularly in agricultural crops, has been largely unsuccessful, as the improvements are frequently accompanied by slower growth and delayed reproduction. here, we ectopically express two abiotic stress-responsive bzip areb/abf transcription factor orthologs ...201728098349
functional characterization of a novel jasmonate zim-domain interactor (ninja) from upland cotton (gossypium hirsutum).the jasmonic acid (ja) signalling pathway plays roles in plant development and defence against biotic and abiotic stresses. we isolated a cotton ninja (novel interactor of ja zim-domain) gene, designated ghninja, which contains a 1305 bp open read frame. the ghninja gene encodes a 434 amino acid peptide. according to quantitative real-time pcr analysis, ghninja is preferentially expressed in roots, and its expression level is greatly induced by verticillium dahliae infection. through a virus-ind ...201728086169
genome-wide identification of the mikc-type mads-box gene family in gossypium hirsutum l. unravels their roles in flowering.cotton is one of the major world oil crops. cottonseed oil meets the increasing demand of fried food, ruminant feed, and renewable bio-fuels. mads intervening keratin-like and c-terminal (mikc)-type mads-box genes encode transcription factors that have crucial roles in various plant developmental processes. nevertheless, this gene family has not been characterized, nor its functions investigated, in cotton. here, we performed a comprehensive analysis of mikc-type mads genes in the tetraploid gos ...201728382045
a gly65val substitution in an actin, ghact_li1, disrupts cell polarity and f-actin organization resulting in dwarf, lintless cotton plants.actin polymerizes to form part of the cytoskeleton and organize polar growth in all eukaryotic cells. species with numerous actin genes are especially useful for the dissection of actin molecular function due to redundancy and neofunctionalization. here, we investigated the role of a cotton (gossypium hirsutum) actin gene in the organization of actin filaments in lobed cotyledon pavement cells and the highly elongated single-celled trichomes that comprise cotton lint fibers. using mapping-by-seq ...201728078746
microrna 157-targeted spl genes regulate floral organ size and ovule production in cotton.micrornas (mirnas) have been involved in regulation of diverse spectrum of plant development processes in many species. in cotton, few mirnas have been well characterised in floral organ development. floral organ, which should be finely tuned, is a crucial factor affecting the yield of cotton. therefore, it is well worth revealing the function of mirnas in regulation of floral organ development. here, we report the role of mirna156/157 in regulation of floral organ size in cotton.201728068913
modifications to a late meristem identity1 gene are responsible for the major leaf shapes of upland cotton (gossypium hirsutum l.).leaf shape varies spectacularly among plants. leaves are the primary source of photoassimilate in crop plants, and understanding the genetic basis of variation in leaf morphology is critical to improving agricultural productivity. leaf shape played a unique role in cotton improvement, as breeders have selected for entire and lobed leaf morphs resulting from a single locus, okra (l-d1), which is responsible for the major leaf shapes in cotton. the l-d1 locus is not only of agricultural importance ...201727999177
molecular characterization of ghpldα1 and its relationship with secondary cell wall thickening in cotton fibers.phospholipase d (pld) hydrolyzes phospholipids to generate a free polar head group (e.g., choline) and a second messenger phosphatidic acid and plays diverse roles in plant growth and development, including seed germination, leaf senescence, root hair growth, and hypocotyl elongation. however, the function of pld in cotton remains largely unexplored. here, the comprehensive molecular characterization of ghpldα1 was explored with its role in upland cotton (gossypium hirsutum) fiber development. t ...201727864277
leaf hydraulic conductance and mesophyll conductance are not closely related within a single species.stomata represent one resistor in a series of resistances for carbon and water exchange between the leaf and the atmosphere; the remaining resistors occurring within the leaf, commonly represented as mesophyll conductance to co2 , gm , and leaf hydraulic conductance, kleaf . recent studies have proposed that gm and kleaf may be coordinated across species because of shared pathways. we assessed the correlation between gm and kleaf within cotton, under growth co2 partial pressure and irradiance tr ...201727861995
enhanced plant growth promoting role of phycomolecules coated zinc oxide nanoparticles with p supplementation in cotton (gossypium hirsutum l.).this report focuses on application of zinc oxide nanoparticles (znonps) carrying phycomolecule ligands as a novel plant growth promoter aimed at increasing the crop productivity. the present investigation examined the effect of znonps on plant growth characteristics, and associated biochemical changes in cotton (gossypium hirsutum l.) following growth in a range of concentrations (25-200 mg l(-l) znonps) in combination with 100 mm p in a hydroponic system. treated plants registered an increase i ...201727622847
quantification of climate warming and crop management impacts on cotton phenology.understanding the impact of the warming trend on phenological stages and phases of cotton (gossypium hirsutum l.) in central and lower punjab, pakistan, may assist in optimizing crop management practices to enhance production. this study determined the influence of the thermal trend on cotton phenology from 1980-2015 in 15 selected locations. the results demonstrated that observed phenological stages including sowing (s), emergence (e), anthesis (a) and physiological maturity (m) occurred earlie ...201728208605
combined elevated temperature and soil waterlogging stresses inhibit cell elongation by altering osmolyte composition of the developing cotton (gossypium hirsutum l.) fiber.soil waterlogging events and high temperature conditions occur frequently in the yangtze river valley, yet the effects of these co-occurring stresses on fiber elongation have received little attention. in the current study, the combined effect of elevated temperature (et) and soil waterlogging (sw) more negatively affected final fiber length (reduced by 5.4%-11.3%) than either stress alone by altering the composition of osmotically active solutes (sucrose, malate, and k(+)), where sw had the mos ...201728167033
anthocyanin accumulation enhanced in lc-transgenic cotton under light and increased resistance to bollworm.breeding of naturally colored cotton fiber has been hampered by the limited germplasm, an alternative way is to use transgenic approach to create more germplasm for breeding. here, we report our effort to engineer anthocyanin production in cotton. the maize lc gene, under the control of the constitutive 35s promoter, was introduced into cotton through genetic transformation. our data showed that the expression of the lc gene alone is sufficient to trigger the accumulation of anthocyanin in a var ...201726941851
the cotton β-galactosyltransferase 1 (galt1) that galactosylates arabinogalactan proteins participates in controlling fiber development.arabinogalactan proteins (agps) are highly glycosylated proteins that play pivotal roles in diverse developmental processes in plants. type-ii ag glycans, mostly o-linked to the hydroxyproline residues of the protein backbone, account for up to 95% w/w of the agp, but their functions are still largely unclear. cotton fibers are extremely elongated single-cell trichomes on the seed epidermis; however, little is known of the molecular basis governing the regulation of fiber cell development. here, ...201727888523
development of molecular markers for genetic male sterility in gossypium hirsutum.genetic male sterility (gms) in cotton mediated by two homozygous recessive genes, ms5ms5 and ms6ms6, is expressed as non-dehiscent anthers and unviable pollen grains. sequence analysis on ms5 and ms6 loci in gossypium hirsutum was conducted to reveal genomic variation at these two loci between gms and wild-type g. hirsutum inbred lines, and sequence polymorphism linked to ms5 on a12 and ms6 on d12 was revealed. a haplotype marker set that consisted of four snps targeting both ms5 and ms6 gene r ...201726074724
comparative analysis of resistance gene analogues encoding nbs-lrr domains in cotton.plant production is severely affected by biotic and abiotic stresses r-genes exhibit resistance against a range of diseases and pathogens in plants. the nucleotide binding site and leucine rich repeat (nbs-lrr) class of r-genes is the most comprehensively studied in terms of sequence evolution and genome distribution. the differential response for resistance against biotic and abiotic stress has been observed in cultivated and wild relatives of the genus gossypium.201625640313
identification of stable quantitative trait loci (qtls) for fiber quality traits across multiple environments in gossypium hirsutum recombinant inbred line population.the identification of quantitative trait loci (qtls) that are stable and consistent across multiple environments and populations plays an essential role in marker-assisted selection (mas). in the present study, we used 28,861 simple sequence repeat (ssr) markers, which included 12,560 gossypium raimondii (d genome) sequence-based ssr markers to identify polymorphism between two upland cotton strains 0-153 and sgk9708. a total of 851 polymorphic primers were finally selected and used to genotype ...201626951621
genetic gains from selection for fiber traits in gossypium hirsutum l.brazil is among the five largest producers of cotton in the world, cultivating the species gossypium hirsutum l. r. latifolium hutch. the cultivars should have good fiber quality as well as yield. genetic improvement of fiber traits requires the study of the genetic structure of the populations under improvement, leading to the identification of promising parent plants. to this end, it is important to acquire some information, such as estimates of genetic variance components and heritability coe ...201627886330
integrated mapping and characterization of the gene underlying the okra leaf trait in gossypium hirsutum l.diverse leaf morphology has been observed among accessions of gossypium hirsutum, including okra leaf, which has advantages and disadvantages in cotton production. the okra leaf locus has been mapped to chromosome 15 of the dt subgenome, but the underlying gene has yet to be identified. in this study, we used a combination of targeted association analysis, f2 population-based fine mapping, and comparative sequencing of orthologues to identify a candidate gene underlying the okra leaf trait in g. ...201626567355
a cotton mirna is involved in regulation of plant response to salt stress.the present study functionally identified a new microrna (microrna ovual line 5, mirnvl5) with its target gene ghchr from cotton (gossypium hirsutum). the sequence of mirnvl5 precursor is 104 nt long, with a well developed secondary structure. ghchr contains two dc1 and three phd cys/his-rich domains, suggesting that ghchr encodes a zinc-finger domain-containing transcription factor. mirnvl5 and ghchr express at various developmental stages of cotton. under salt stress (50-400 mm nacl), mirnvl5 ...201626813144
stable integration and expression of a cry1ia gene conferring resistance to fall armyworm and boll weevil in cotton plants.boll weevil is a serious pest of cotton crop. effective control involves applications of chemical insecticides, increasing the cost of production and environmental pollution. the current genetically modified bt crops have allowed great benefits to farmers but show activity limited to lepidopteran pests. this work reports on procedures adopted for integration and expression of a cry transgene conferring resistance to boll weevil and fall armyworm by using molecular tools.201626558603
arabidopsis edt1/hdg11 improves drought and salt tolerance in cotton and poplar and increases cotton yield in the field.drought and salinity are two major environmental factors limiting crop production worldwide. improvement of drought and salt tolerance of crops with transgenic approach is an effective strategy to meet the demand of the ever-growing world population. arabidopsis enhanced drought tolerance1/homeodomain glabrous11 (atedt1/hdg11), a homeodomain-start transcription factor, has been demonstrated to significantly improve drought tolerance in arabidopsis, tobacco, tall fescue and rice. here we report t ...201625879154
effect of the postfeeding interval on olfactory responses of thrips to herbivore-induced cotton plants.we investigated the responses of 3 thrips species, frankliniella schultzei trybom, f. occidentalis pergrande, and thrips tabaci lindeman (thysanoptera: thripidae) to herbivore-damaged and undamaged cotton seedlings (gossypium hirsutum l. [malvales: malvaceae]) at a range of time intervals following damage by adult tetranychus urticae (koch), adult t. ludeni (zacher) (acari: tetranychidae) or helicoverpa armigera (hübner) (lepidoptera: noctuidae) larvae in olfactometer assays. the intensity/frequ ...201626172119
cotton square morphology offers new insights into host plant resistance to cotton fleahopper (hemiptera: miridae) in upland cotton.cotton fleahopper, pseudatomoscelis seriatus (reuter) (hemiptera: miridae), is a piercing-sucking pest of cotton (gossypium hirsutum l.) that feeds preferentially on developing flower buds, called squares. heavy infestations cause yield reductions that result from abscission of squares damaged by the cotton fleahopper feeding. antixenosis, or nonpreference, has been reported as a mechanism of host plant resistance in cotton to cotton fleahopper. square structure, particularly the placement of th ...201626475922
proteomic responses of drought-tolerant and drought-sensitive cotton varieties to drought stress.drought, one of the most widespread factors reducing agricultural crop productivity, affects biological processes such as development, architecture, flowering and senescence. although protein analysis techniques and genome sequencing have made facilitated the proteomic study of cotton, information on genetic differences associated with proteomic changes in response to drought between different cotton genotypes is lacking. to determine the effects of drought stress on cotton seedlings, we used tw ...201626941218
identification and functional analysis of micrornas involved in the anther development in cotton genic male sterile line yu98-8a.hybrid vigor contributes in a large way to the yield and quality of cotton (gossypium hirsutum) fiber. although micrornas play essential regulatory roles in flower induction and development, it is still unclear if micrornas are involved in male sterility, as the regulatory molecular mechanisms of male sterility in cotton need to be better defined. in this study, two independent small rna libraries were constructed and sequenced from the young buds collected from the sporogenous cell formation to ...201627739413
ghabf2, a bzip transcription factor, confers drought and salinity tolerance in cotton (gossypium hirsutum l.).the bzip transcription factor (tf) act as an important regulator for the abscisic acid (aba) mediated abiotic stresses signaling pathways in plants. here, we reported the cloning and characterization of ghabf2, encoding for typical cotton bzip tf. overexpression of ghabf2 significantly improved drought and salt stress tolerance both in arabidopsis and cotton. however, silencing of ghabf2 made transgenic cotton sensitive to peg osmotic and salt stress. expression of ghabf2 was induced by drought ...201627713524
na(+) compartmentalization related to salinity stress tolerance in upland cotton (gossypium hirsutum) seedlings.the capacity for ion compartmentalization among different tissues and cells is the key mechanism regulating salt tolerance in plants. in this study, we investigated the ion compartmentalization capacity of two upland cotton genotypes with different salt tolerances under salt shock at the tissue, cell and molecular levels. we found that the leaf glandular trichome could secrete more salt ions in the salt-tolerant genotype than in the sensitive genotype, demonstrating the excretion of ions from ti ...201627698468
histone modifications define expression bias of homoeologous genomes in allotetraploid cotton.histone modifications regulate gene expression in eukaryotes, but their roles in gene expression changes in interspecific hybrids or allotetraploids are poorly understood. histone modifications can be mapped by immunostaining of metaphase chromosomes at the single cell level and/or by chromatin immunoprecipitation-sequencing (chip-seq) for analyzing individual genes. here, we comparatively analyzed immunostained metaphase chromosomes and chip-seq of individual genes, which revealed a chromatin b ...201627637746
susceptibility to insecticides and activities of glutathione s-transferase and esterase in populations of lygus lineolaris (hemiptera: miridae) in mississippi.lygus lineolaris (palisot de beauvois) is a serious pest of cotton (gossypium hirsutum l.) in mississippi, particularly in the delta region. this may be due to decreased insecticide susceptibility in that region. research has revealed populations of l. lineolaris in the delta region with high levels of insecticide resistance; however, comparisons with populations in the remainder of the state are limited.201626598042
transgenic cotton plants expressing cry1ia12 toxin confer resistance to fall armyworm (spodoptera frugiperda) and cotton boll weevil (anthonomus grandis).gossypium hirsutum (commercial cooton) is one of the most economically important fibers sources and a commodity crop highly affected by insect pests and pathogens. several transgenic approaches have been developed to improve cotton resistance to insect pests, through the transgenic expression of different factors, including cry toxins, proteinase inhibitors, and toxic peptides, among others. in the present study, we developed transgenic cotton plants by fertilized floral buds injection (through ...201626925081
cloning and functional analysis of the promoter of an ascorbate oxidase gene from gossypium hirsutum.apoplastic ascorbate oxidase (ao) plays significant roles in plant cell growth. however, the mechanism of underlying the transcriptional regulation of ao in gossypium hirsutum remains unclear. here, we obtained a 1,920-bp promoter sequence from the gossypium hirsutum ascorbate oxidase (ghao1) gene, and this ghao1 promoter included a number of known cis-elements. promoter activity analysis in overexpressing pghao1::gfp-gus tobacco (nicotiana benthamiana) showed that the ghao1 promoter exhibited h ...201627597995
identification and characterization of the ghhsp20 gene family in gossypium hirsutum.in higher plants, heat shock protein 20 (hsp20) plays crucial roles in growth, development and responses to abiotic stresses. in this study, 94 ghhsp20 genes were identified in g. hirsutum, and these genes were phylogenetically clustered into 14 subfamilies. out of these, 73 paralogous gene pairs remained in conserved positions on segmental duplicated blocks and only 14 genes clustered into seven tandem duplication event regions. transcriptome analysis showed that 82 ghhsp20 genes were expressed ...201627580529
global analysis of the developmental dynamics of gossypium hirsutum based on strand-specific transcriptome.cotton is an economically important crop that provides both natural fiber and by-products such as oil and protein. its global gene expression could provide insight into the biological processes underlying growth and development, which involve suites of genes expressed with temporal and spatial control by regulatory networks. generally, the goal for cotton breeding is improvement of the fiber; thus, most previous research has focused on identifying genes specific to the fiber. however, seeds may ...201626892265
diversity in betasatellites associated with cotton leaf curl disease during source-to-sink movement through a resistant host.cotton leaf curl is devastating disease of cotton characterized by leaf curling, vein darkening and enations. the disease symptoms are induced by dna satellite known as cotton leaf curl multan betasatellite (clcumub), dominant betasatellite in cotton but another betasatellite known as chili leaf curl betasatellite (chlcb) is also found associated with the disease. grafting experiment was performed to determine if host plant resistance is determinant of dominant population of betasatellite in cot ...201626889114
Displaying items 101 - 200 of 1942