Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| enzyme activities and arylsulfatase protein content of dust and the soil source: biochemical fingerprints? | little is known about the potential of enzyme activities, which are sensitive to soil properties and management, for the characterization of dust properties. enzyme activities may be among the dust properties key to identifying the soil source of dust. we generated dust (27 and 7 microm) under controlled laboratory conditions from agricultural soils (0-5 cm) with history of continuous cotton (gossypium hirsutum l.) or cotton rotated with peanut (arachis hypogaea l.), sorghum [sorghum bicolor (l. ... | 2006 | 15356225 |
| how do leaf hydraulics limit stomatal conductance at high water vapour pressure deficits? | a reduction in leaf stomatal conductance (g) with increasing leaf-to-air difference in water vapour pressure (d) is nearly ubiquitous. ecological comparisons of sensitivity have led to the hypothesis that the reduction in g with increasing d serves to maintain leaf water potentials above those that would cause loss of hydraulic conductance. a reduction in leaf water potential is commonly hypothesized to cause stomatal closure at high d. the importance of these particular hydraulic factors was te ... | 2006 | 16898024 |
| expression of cp4 epsps in microspores and tapetum cells of cotton (gossypium hirsutum) is critical for male reproductive development in response to late-stage glyphosate applications. | plants expressing agrobacterium sp. strain cp4 5-enolpyruvylshikimate-3-phosphate synthase (cp4 epsps) are known to be resistant to glyphosate, a potent herbicide that inhibits the activity of the endogenous plant epsps. the rr1445 transgenic cotton line (current commercial line for roundup ready cotton) was generated using the figwort mosaic virus (fmv) 35s promoter to drive the expression of the cp4 epsps gene, and has excellent vegetative tolerance to glyphosate. however, with high glyphosate ... | 2006 | 17309724 |
| characterization and expression of a putative retinoblastoma protein binding gene from gossypium hirsutum. | a genomic clone representing a putative retinoblastoma binding (rbb) protein was isolated from a gossypium hirsutum bac library. alignment of the gene sequence with the cdna sequence indicated the gene consists of six exons that have standard eukaryotic splice junctions. the conceptual spliced transcript was 98% identical to tc37171 in the tigr gene index, however it encoded an orf 107 amino acids longer than best deduced protein from tc37171. the conceptual translation of the genomic clone was ... | 2006 | 17312951 |
| cloning and characterization of cotton ghbg gene encoding beta-glucosidase. | beta-1,4-glucosidase (bg, ec3.2.1.21), one of three cellulases, is a widespread family of enzymes involved in the metabolism of cell wall polysaccharides in both prokaryocytes and eukaryotes. here, we report the isolation of a full-length cdna encoding beta-1,4-glucosidase protein (designated as ghbg) and its putative function in the process of fiber development and in yeast. through random sequencing of the cotton fiber cdna library from 7235 germplasm line, with elite fiber quality in gossypiu ... | 2006 | 17343209 |
| dna screening reveals pink bollworm resistance to bt cotton remains rare after a decade of exposure. | transgenic crops producing toxins from the bacterium bacillus thuringiensis (bt) kill insect pests and can reduce reliance on insecticide sprays. although bt cotton (gossypium hirsutum l.) and bt corn (zea mays l.) covered 26 million ha worldwide in 2005, their success could be cut short by evolution of pest resistance. monitoring the early phases of pest resistance to bt crops is crucial, but it has been extremely difficult because bioassays usually cannot detect heterozygotes harboring one all ... | 2006 | 17066779 |
| genetic variation for resistance to bacillus thuringiensis toxins in helicoverpa zea (lepidoptera: noctuidae) in eastern north carolina. | to evaluate resistance to bacillus thuringiensis berliner (bt) toxins, adult female bollworms, helicoverpa zea (boddie) (lepidoptera: noctuidae), were collected from four light trap locations in two eastern north carolina counties from august to october during 2001 and 2002. females were allowed to oviposit, and upon hatching, 24 neonates from each female (f1 lines) were screened for survival and growth rate on each of three diets: non-bt diet, diet containing 5.0 microg/ml cry1ac toxin, or diet ... | 2006 | 17066814 |
| effect of cotton cultivar on performance of aphis gossypii (homoptera: aphididae) in iran. | cotton aphids, aphis gossypii glover (homoptera: aphididae), obtained from cotton, gossypium hirsutum l., fields in the gorgan region of northern iran, were colonized on 'varamin' cotton plants in a growth chamber. the development, survivorship, and life table parameters of the cotton aphid were evaluated at 27.5 +/- 1 degrees c, 65 +/- 10% rh, and aphotoperiod of 14:10 (l:d) h of artificial light on five commonly growing cotton cultivars: varamin, 'sealand' (relatively resistant cultivar), 'bak ... | 2006 | 17066818 |
| selecting for efficacy of bollgard cotton cultivars against various lepidoptera using forward breeding techniques. | studies during the past 5 yr have shown that the overall level of protein (cry1ac) produced from the cry1ac transgene (monsanto co., st. louis, mo) differ among commercial bollgard cotton, gossypium hirsutum l., cultivars. these differences between cultivars are under genetic control and have been correlated with efficacy of certain lepidopteran pests. previous studies have shown that the parental background (i.e., non-cry1ac conventional cultivar) has a significant influence on the amount of cr ... | 2006 | 17066820 |
| [isolation by suppression-subtractive hybridization of genes preferentially expressed during early and late fiber development stages in cotton]. | as a main natural fiber source, cotton plays an important role in human life. to identify genes preferentially expressed during early and late cotton fiber development, we constructed two fiber subtracted libraries on the basis of pcr-selected subtraction using a pool of nonfiber tissues as the same driver and 10 days postanthesis (dpa) and 20 dpa fiber cells as testers, respectively. through differential screening, 292 clones in both libraries were identified as being preferentially expressed d ... | 2006 | 17086983 |
| cadherin-based resistance to bacillus thuringiensis cotton in hybrid strains of pink bollworm: fitness costs and incomplete resistance. | recessive resistance to bacillus thuringiensis (bt) cotton, gossypium hirsutum l., in laboratory-selected strains of pink bollworm, pectinophora gossypiella (saunders), is associated with three resistance alleles (r1, r2, and r3) of a cadherin gene. previous experiments based on measurement of fitness components in bt-resistant and bt-susceptible strains revealed that fitness costs and incomplete resistance are associated with resistance. here, we used two hybrid strains of pink bollworm, each c ... | 2006 | 17195656 |
| modeling the impact of alternative hosts on helicoverpa zea adaptation to bollgard cotton. | for highly polyphagous cotton, gossypium hirsutum l., pests such as helicoverpa zea (boddie), a substantial portion of the larval population develops on noncotton alternative hosts. these noncotton hosts potentially provide a natural refuge for h. zea, thereby slowing the evolution of resistance to the bacillus thuringiensis berliner (bt)-derived cry1ac protein present in bollgard cotton. here, we demonstrate how the measured contribution of such alternative hosts can be included in estimating t ... | 2006 | 17195681 |
| high-level resistance to bacillus thuringiensis toxin crylac and cadherin genotype in pink bollworm. | resistance to transgenic cotton, gossypium hirsutum l., producing bacillus thuringiensis (bt) toxin cry1ac is linked with three recessive alleles of a cadherin gene in laboratory-selected strains of pink bollworm, pectinophora gossypiella (saunders), a major cotton pest. here, we analyzed a strain (mov97-r) with a high frequency of cadherin resistance alleles, a high frequency of resistance to 10 microg of cry1ac per milliliter of diet, and an intermediate frequency of resistance to 1000 microg ... | 2006 | 17195682 |
| diversity of boll weevil populations in south america: a phylogeographic approach. | a phylogeographic approach was conducted to assess the geographic structure and genetic variation in populations of the boll weevil anthonomus grandis, which is the most harmful insect pest of cotton in the americas. coi and coii mitochondrial gene sequences were analyzed to test a former hypothesis on the origin of the boll weevil in argentina, brazil and paraguay, using samples from mexico and usa as putative source populations. the analysis of variability suggests that populations from south ... | 2006 | 16636929 |
| cotton (gossypium hirsutum l.). | considering the economic importance of cotton in many developing and developed countries, there is an urgent need to accelerate the application of biotechnological tools to address the problems associated with the production of this crop and to improve the quality of fiber and seed. this requires a simple yet robust gene delivery/transformant recovery system. a protocol for the production of transgenic cotton plants was refined in our laboratory. it involves agrobacterium-mediated transformation ... | 2006 | 16988351 |
| cloning and characterization of two erebp transcription factors from cotton (gossypium hirsutum l.). | in this research, two erebp (ethylene response element binding protein) genes were isolated by the yeast one-hybrid system, named ghereb2 and ghereb3, and both have one intron in their coding regions. the deduced amino acid sequences of ghereb2 and ghereb3 have some typical features of transcription factors, one potential basic nuclear-localization signal, one possible acidic activation domain, and one conserved dna binding domain, and they show high similarity, especially in the dna-binding dom ... | 2006 | 16545065 |
| the complete chloroplast genome sequence of gossypium hirsutum: organization and phylogenetic relationships to other angiosperms. | cotton (gossypium hirsutum) is the most important fiber crop grown in 90 countries. in 2004-2005, us farmers planted 79% of the 5.7-million hectares of nuclear transgenic cotton. unfortunately, genetically modified cotton has the potential to hybridize with other cultivated and wild relatives, resulting in geographical restrictions to cultivation. however, chloroplast genetic engineering offers the possibility of containment because of maternal inheritance of transgenes. the complete chloroplast ... | 2006 | 16553962 |
| metabolism of [14c]-2,4-dichlorophenol in edible plants. | several 2,4-dichlorophenoxyacetic acid (2,4-d)-sensitive plants have been modified by genetic engineering with tfda gene to acquire 2,4-d tolerance. the expression product of this gene degrades 2,4-d to 2,4-dichlorophenol (dcp), which is less phytotoxic but could cause a problem of food safety. after a comparison of 2,4-d and dcp metabolism in transgenic 2,4-d-tolerant and wild cotton (gossypium hirsutum l.), a direct study of dcp metabolism in edible plants was performed. after petiolar uptake ... | 2006 | 16628540 |
| wide-cross whole-genome radiation hybrid mapping of the cotton (gossypium barbadense l.) genome. | whole-genome radiation hybrid mapping has been applied extensively to human and certain animal species, but little to plants. we recently demonstrated an alternative mapping approach in cotton (gossypium hirsutum l.), based on segmentation by 5-krad gamma-irradiation and derivation of wide-cross whole-genome radiation hybrids (wwrhs). however, limitations observed at the 5-krad level suggested that higher doses might be advantageous. here, we describe the development of an improved second-genera ... | 2006 | 16362372 |
| isolation of a cotton reversibly glycosylated polypeptide (ghrgp1) promoter and its expression activity in transgenic tobacco. | reversibly glycosylated polypeptides (rgps) are thought to be involved in polysaccharide metabolism. a cdna of the cotton (gossypium hirsutum) rgp gene, designated ghrgp1, has previously been characterized, and is preferentially expressed in fiber cells. in order to investigate its temporal and spatial control, we isolated a 624bp fragment upstream of the ghrgp1 coding sequence using a polymerase chain reaction (pcr)-based genomic walking method, transcriptionally fused the 624bp promoter sequen ... | 2006 | 16455356 |
| molecular variability of spodoptera frugiperda (lepidoptera: noctuidae) populations associated to maize and cotton crops in brazil. | the molecular variability among 10 populations of spodoptera frugiperda (j.e. smith), collected from maize, zea mays l., or cotton gossypium hirsutum l. crops located at distinctive geographical regions in brazil, was assessed through random amplification of polymorphic dna (rapd)-polymerase chain reaction (pcr). in total, 208 rapd markers were evaluated, and 98% of them were polymorphic. the mean genetic similarity was 0.6621 and 0.2499 by the simple matching and jaccard matrices, respectively. ... | 2006 | 16686155 |
| analysis of ests from multiple gossypium hirsutum tissues and identification of ssrs. | in an effort to expand the gossypium hirsutum l. (cotton) expressed sequence tag (est) database, ests representing a variety of tissues and treatments were sequenced. assembly of these sequences with ests already in the est database (dbest, genbank) identified 9675 cotton sequences not present in genbank. statistical analysis of a subset of these ests identified genes likely differentially expressed in stems, cotyledons, and drought-stressed tissues. annotation of the differentially expressed cd ... | 2006 | 16699550 |
| isolation of the promoter of a cotton beta-galactosidase gene (ghgal1) and its expression in transgenic tobacco plants. | beta-galactosidases (ec 3.2.1.23) constitute a widespread family of glycosyl hydrolases in plants and are thought to be involved in metabolism of cell wall polysaccharides. a cdna of the cotton (gossypium hirsutum) beta-galactosidase gene, designated ghgal1, has previously been identified and its transcripts are highly abundant at the elongation stage of the cotton fiber. to examine the temporal and spatial control of ghgal1 expression, a transcriptional fusion of the ghgal1 promoter region (177 ... | 2006 | 16704113 |
| an experiment using neutron activation analysis and a rare earth element to mark cotton plants and two insects that feed on them. | studies on insect dispersal and other behaviors can benefit from using markers that will not alter flight and fitness. rare earth elements, such as samarium (sm), have been used as ingested markers of some insects and detected using neutron activation analysis (naa). in this study, samarium nitrate hexahydrate was mixed into artificial diet for boll weevils, anthonomus grandis grandis boheman (coleoptera: curculionidae), at different dosages and in water used to irrigate cotton, gossypium hirsut ... | 2006 | 16713273 |
| the cloning and sequencing of a cdna encoding a wd repeat protein in cotton (gossypium hirsutum l.). | in this research, one 1156 bp cdna containing full open reading frame and encoding a novel 24-kda protein with four tandem wd repeat motifs was cloned from cotton, therefore was named ghwdr and the genbank accession number is ay870657. by search of ghwdr cdna and amino acid sequences in the database, we found that ghwdr and osjnba0003g23.2 from oryza sativa show 90% sequence identity and 84% identity to wd-repeat protein from arabidopsis thaliana, and also has high sequence identity to other wd ... | 2006 | 16753817 |
| glyphosate-induced anther indehiscence in cotton is partially temperature dependent and involves cytoskeleton and secondary wall modifications and auxin accumulation. | yield reduction caused by late application of glyphosate to glyphosate-resistant cotton (gossypium hirsutum; grc) expressing cp4 5-enol-pyruvylshikmate-3-p synthase under the cauliflower mosaic virus-35s promoter has been attributed to male sterility. this study was aimed to elucidate the factors and mechanisms involved in this phenomenon. western and tissue-print blots demonstrated a reduced expression of the transgene in anthers of grc compared to ovules of the same plants. glyphosate applicat ... | 2006 | 16766672 |
| agrobacterium-mediated transformation of cotton (gossypium hirsutum l. cv. zhongmian 35) using glyphosate as a selectable marker. | the most economically significant chinese cotton cultivar (gossypium hirsutum l. cv. zhongmian 35) was transformed via agrobacterium tumefaciens-mediated dna transfer. the aroa-m1 gene that confers resistance to the glyphosate was fused with a chloroplast-transit peptide of arabidopsis thaliana 5-enolpyruvyl-3-phosphoshikimate synthase (asp) and expressed in cotton plants under the control of a camv35s promoter. transgenic plants were directly selected on medium containing glyphosate. thirty-fou ... | 2006 | 16799756 |
| the absolute configuration of (-)-3-hydroxy-alpha-calacorene. | 3-hydroxy-alpha-calacorene was identified in extracts from cold-shocked seedlings of cotton (gossypium hirsutum l.) and kenaf (hibiscus cannabinus l.), both of which are members of the malvaceae family. (-)-3-hydroxy-alpha-calacorene was isolated from heterotheca inuloides cass. (asteraceae). hplc on a chiral stationary phase column showed that the 3-hydroxy-alpha- calacorene from cotton and kenaf had the same relative configuration, while that from h. inuloides was of the opposite configuration ... | 2006 | 16806327 |
| captures of boll weevils (coleoptera: curculionidae) in traps associated with different habitats. | programs to eradicate the boll weevil, anthonomus grandis grandis boheman, from cotton, gossypium hirsutum l., in the united states rely heavily on pheromone traps for monitoring weevil populations in both active and posteradication maintenance programs. modifications to trapping protocols that increase trap effectiveness should contribute to this eradication effort. between october 1996 and may 1997 and between september 1997 and april 1998, we compared trap effectiveness, indicated by the numb ... | 2006 | 16813308 |
| effect of resistance to bacillus thuringiensis cotton on pink bollworm (lepidoptera: gelechiidae) response to sex pheromone. | fitness costs associated with resistance to transgenic crops producing toxins from bacillus thuringiensis (bt) could reduce male response to pheromone traps. such costs would cause underestimation of resistance frequency if monitoring was based on analysis of males caught in pheromone traps. to develop a dna-based resistance monitoring program for pink bollworm, pectinophora gossypiella (saunders) (lepidoptera: gelechiidae), we compared the response to pheromone traps of males with and without c ... | 2006 | 16813335 |
| carbon supply and storage in tilled and nontilled soils as influenced by cover crops and nitrogen fertilization. | soil carbon (c) sequestration in tilled and nontilled areas can be influenced by crop management practices due to differences in plant c inputs and their rate of mineralization. we examined the influence of four cover crops {legume [hairy vetch (vicia villosa roth)], nonlegume [rye (secale cereale l.)], biculture of legume and nonlegume (vetch and rye), and no cover crops (or winter weeds)} and three nitrogen (n) fertilization rates (0, 60 to 65, and 120 to 130 kg n ha(-1)) on c inputs from cove ... | 2006 | 16825471 |
| the cotton fiber zinc-binding domain of cellulose synthase a1 from gossypium hirsutum displays rapid turnover in vitro and in vivo. | little is known about the assembly and turnover of cellulose synthase complexes commonly called rosettes. recent work indicates that rosette assembly could involve the dimerization of cesa (cellulose synthase catalytic subunit) proteins regulated by the redox state of the cesa zinc-binding domain (znbd). several studies in the 1980s led to the suggestion that synthase complexes may have very short half-lives in vivo, but no recent work has directly addressed this issue. in the present work, we s ... | 2006 | 16873546 |
| the current status and environmental impacts of glyphosate-resistant crops: a review. | glyphosate [n-(phosphonomethyl) glycine]-resistant crops (grcs), canola (brassica napus l.), cotton (gossypium hirsutum l.), maize (zea mays l.), and soybean [glycine max (l.) merr.] have been commercialized and grown extensively in the western hemisphere and, to a lesser extent, elsewhere. glyphosate-resistant cotton and soybean have become dominant in those countries where their planting is permitted. effects of glyphosate on contamination of soil, water, and air are minimal, compared to some ... | 2006 | 16899736 |
| cloning and functional analysis of the novel gene ghdbp3 encoding a dre-binding transcription factor from gossypium hirsutum. | a novel cdna encoding dre-binding transcription factor, designated ghdbp3, was cloned from gossypium hirsutum. this protein was classified into a-4 group of dreb subfamily based on multiple sequence alignment and phylogenetic characterization. semiquantitative rt-pcr showed that ghdbp3 was expressed in the leaves, cotyledons, roots and stems of 2-week-old cotton seedlings under non-stress conditions and was greatly induced in the cotton cotyledons by drought, nacl, low temperature and aba treatm ... | 2006 | 16935362 |
| short-range dispersal and overwintering habitats of boll weevils (coleoptera: curculionidae) during and after harvest in the subtropics. | field experiments in the subtropical lower rio grande valley of texas were conducted to determine the extent of adult boll weevil, anthonomus grandis grandis boheman (coleoptera: curculionidae), dispersal from cotton, gossypium hirsutum l., fields during harvest operations and the noncotton-growing ("overwinter") period between 1 september and 1 february. using unbaited large capacity boll weevil traps placed at intervals extending outward from commercial field edges, boll weevils did not move i ... | 2006 | 16937667 |
| detection by rapd-pcr of polymorphism in populations of bemisia tabaci (genn.) collected on four host plants from iran. | the sweetpotato whitefly, bernisia tabaci (genn.) (hom: aleyrodidae) is a major pest of field crops, vegetables and ornamentals in iran. in this study, the infested leaves of cucumber (cucurnis sativus l.) zucchini (cucurbita pepo l.) eggplant (solanum melongena l.) and cotton (gossypium hirsutum l.) with whitefly nymphs and pupae were collected from iran, and were transferred to the laboratory. the newly emerged males and females of each population were released separately into a large cage set ... | 2006 | 17385530 |
| fusarium wilt (fusarium oxysporum f. sp. vasinfectum) genes expressed during infection of cotton (gossypium hirsutum)dagger. | summary we sought to identify fusarium oxysporum f. sp. vasinfectum (fov) genes that may be associated with pathogenicity. initially we utilized microarray and q-pcr technology to identify fov genes expressed in root and hypocotyl tissues during a compatible infection of cotton. we identified 218 fungal clones representing 174 fov non-redundant genes as expressed in planta. the majority of the expressed sequences were expressed in infected roots, with only six genes detected in hypocotyl tissue. ... | 2006 | 20507430 |
| qtl mapping of yield and yield components for elite hybrid derived-rils in upland cotton. | a population of 180 recombinant inbred lines (rils) was developed by single seed descended from the cross of high yield upland cotton (gossypium hirsutum l.) varieties zhongmiansuo12 (zms12) and 8891, the two parents of xiangzamian2 (xzm2). a genetic linkage map consisting of 132 loci and covering 865.20 cm was constructed using the ril population chiefly with ssr markers. yield and yield components were investigated for rils in three environments in china. the purpose of the present research wa ... | 2007 | 17469776 |
| saturated very-long-chain fatty acids promote cotton fiber and arabidopsis cell elongation by activating ethylene biosynthesis. | fatty acids are essential for membrane biosynthesis in all organisms and serve as signaling molecules in many animals. here, we found that saturated very-long-chain fatty acids (vlcfas; c20:0 to c30:0) exogenously applied in ovule culture medium significantly promoted cotton (gossypium hirsutum) fiber cell elongation, whereas acetochlor (2-chloro-n-[ethoxymethyl]-n-[2-ethyl-6-methyl-phenyl]-acetamide; ace), which inhibits vlcfa biosynthesis, abolished fiber growth. this inhibition was overcome b ... | 2007 | 17993622 |
| detection and characterization of cry1ac transgene construct in bt cotton: multiple polymerase chain reaction approach. | to comply with international labeling regulations for genetically modified (gm) crops and food, and to enable proper identification of gm organisms (gmos), effective methodologies and reliable approaches are needed. the spurious and unapproved gm planting has contributed to crop failures and commercial losses. to ensure effective and genuine gm cultivation, a methodology is needed to detect and identify the trait of interest and concurrently evaluate the structural and functional stability of th ... | 2007 | 18193727 |
| cloning and expression of the chloroplast copper/zinc-superoxide dismutase gene in upland cotton (gossypium hirsutum l.). | a full-length 1,043-base-pair cdna clone encoding a chloroplast copper/zinc superoxide dismutase (cu/zn-sod) of upland cotton was first isolated by rapid amplification of cdna ends (race) from the leaves of the cotton (gossypium hirsutum l.) variety 'cri36'. nucleotide sequence analysis of the clone revealed that it contained the complete coding sequence of the mature sod isozyme subunit, along with a 60-amino acid transit peptide at n-terminal. the amino acid sequence predicted from the full-le ... | 2007 | 17556806 |
| a novel map kinase gene in cotton (gossypium hirsutum l.), ghmapk, is involved in response to diverse environmental stresses. | the mitogen-activated protein kinase (mapk) cascade is one of the major and evolutionally conserved signaling pathways and plays pivotal role in the regulation of stress and developmental signals in plants. here, a novel gene, termed gossypium hirsutum mapk (ghmapk), was isolated from cotton. the full-length cdna of ghmapk encodes for a 372 amino acid protein that contains all 11 of the mapk conserved subdomains and the phosphorylation-activation motif, tey. amino acid sequence alignment reveale ... | 2007 | 17562283 |
| investigation of some yield and fibre quality characteristics of interspecific hybrid (gossypium hirsutum l. x g. barbadense l.) cotton varieties. | interspecific hybrid cottons (gossypium hirsutum l. x g. barbadense l.) have great both yield and quality potential. this study was conducted to determine potential yields and quality characteristics of hybrid cotton varieties in southeastern anatolia region of turkey. the experiment was set out a completely randomized block design with four replications during 2003 and 2004 at university of dicle, faculty of agriculture experimental field. seven interspecific hybrid cotton varieties (48-08, sev ... | 2007 | 17567438 |
| differentiating tobacco budworm and corn earworm using near-infrared spectroscopy. | near-infrared spectroscopy (nirs) was used to develop a simple and quick technique to differentiate two economically important species, the tobacco budworm, heliothis cirescens (f.), and corn earworm, helicoverpa zea (boddie), which are major pests of cotton, gossypium hirsutum l., in the southern united states. in practice, it is difficult to distinguish the two species during their immature stages using morphological characteristics unless expensive microscopy equipment or trained technicians ... | 2007 | 17598536 |
| resistance to bacillus thuringiensis toxin cry2ab in a strain of helicoverpa armigera (lepidoptera: noctuidae) in australia. | transgenic cotton, gossypium hirsutum l., expressing the crylac and cry2ab genes from bacillus thuringiensis (bt) berliner variety kurstaki in a pyramid (bollgard ii) was widely planted for the first time in australia during the 2004-2005 growing season. before the first commercial bollgard ii crops, limited amounts of cotton expressing only the crylac gene (ingard) was grown for seven seasons. no field failures due to resistance to crylac toxin were observed during that period and a monitoring ... | 2007 | 17598553 |
| mating compatibility among helicoverpa armigera (lepidoptera: noctuidae) occurring on selected host plants and bt cotton survivors. | helicoverpa armigera (hiibner) (lepidoptera: noctuidae) is a well-known polyphagous insect pest. mating compatibility among the insects occurring on different host plants is essential for free gene flow among populations. we tested the extent of crossability and fecundity of the insects that survived on bacillus thuringiensis (bt) cotton with those occurring on pigeon pea, cajanus cajun (l.) millsp., non-bt cotton, gossypium hirsutum l.; sunflower, helianthus annuus; sorghum, sorghum bicolor l. ... | 2007 | 17598554 |
| cross-resistance studies of cry1ac-resistant strains of helicoverpa armigera (lepidoptera: noctuidae) to cry2ab. | bioassays and binding tests between cry toxins (crylaa, crylab, crylac, and cry2ab) and brush-border membrane vesicles (bbmvs) from larvae of a bacillus thurningiensis (bt)-susceptible (96s) and two crylac-resistant strains (btr and lfr10) were conducted for investigating cross-resistance of crylac-resistant strains to cry2ab in helicoverpa armigera (hiibner) (lepidoptera: noctuidae). the resistance ratio (rr) values of the btr and lfr10 strains to crylac and cry2ab were 2,971- and 1.1-fold and ... | 2007 | 17598555 |
| cloning of a mads box gene (ghmads3) from cotton and analysis of its homeotic role in transgenic tobacco. | a mads box gene (ghmads3) was cloned from cotton (gossypium hirsutum l.) based on est sequences. the predicted protein sequence of ghmads3 showed 85%, 73%, and 62% identity with theobroma cacao tcag, antirrhinum majus far, and arabidopsis thaliana ag, respectively, and was grouped with ag homologues when the full length sequences excluding n-extensions were compared. ghmads3 expressed in the wild type cotton flower primarily in stamens and carpels, which was comparable to ag in arabidopsis. howe ... | 2007 | 17601612 |
| the 9-lipoxygenase ghlox1 gene is associated with the hypersensitive reaction of cotton gossypium hirsutum to xanthomonas campestris pv malvacearum. | hypersensitive reaction (hr) cell death of cotton to the incompatible race 18 from xanthomonas campestris pathovar malvacearum (xcm) is associated with 9s-lipoxygenase activity (lox) responsible for lipid peroxidation. here, we report the cloning of cotton (gossypium hirsutum l.) lox gene (ghlox1) and the sequencing of its promoter. ghlox1 was found to be highly expressed during xcm induced hr. sequence analysis showed that ghlox1 is a putative 9-lox, and ghlox1 promoter contains sa and ja respo ... | 2007 | 17611116 |
| a cotton ascorbate peroxidase is involved in hydrogen peroxide homeostasis during fibre cell development. | reactive oxygen species (ros) play important roles in multiple physiological processes such as cellular signalling and stress responses, whereas, the hydrogen peroxide (h(2)o(2)) scavenging enzyme ascorbate peroxidase (apx) participates in the regulation of intracellular ros levels. here, a cotton (gossypium hirsutum) cytosolic apx1 (ghapx1) was identified to be highly accumulated during cotton fibre elongation by proteomic analysis. ghapx1 cdna contained an open reading frame of 753-bp encoding ... | 2007 | 17635221 |
| transmission of cotton seed and boll rotting bacteria by the southern green stink bug (nezara viridula l.). | to determine the ability of the southern green stink bug (sgsb) (nezara viridula l.) to transmit pantoea agglomerans into cotton (gossypium hirsutum) bolls. | 2007 | 17650204 |
| host plants of the tarnished plant bug (heteroptera: miridae) in central texas. | the tarnished plant bug, lygus lineolaris (palisot de beauvois), has taken on added importance as a pest of cotton in the cotton belt after successful eradication efforts for the boll weevil (anthonomus grandis grandis boheman). because the southern blacklands region of central texas is in advanced stages of boll weevil eradication, blooming weeds and selected row crops were sampled during a 3-yr study to determine lygus species composition and associated temporal host plants. l. lineolaris was ... | 2007 | 17716463 |
| variable rainfall intensity and tillage effects on runoff, sediment, and carbon losses from a loamy sand under simulated rainfall. | the low-carbon, intensively cropped coastal plain soils of georgia are susceptible to runoff, soil loss, and drought. reduced tillage systems offer the best management tool for sustained row crop production. understanding runoff, sediment, and chemical losses from conventional and reduced tillage systems is expected to improve if the effect of a variable rainfall intensity storm was quantified. our objective was to quantify and compare effects of a constant (ic) intensity pattern and a more real ... | 2007 | 17766829 |
| cotton ghdreb1 increases plant tolerance to low temperature and is negatively regulated by gibberellic acid. | the transcription factors c-repeat binding factors/dehydration-responsive element binding proteins (cbfs/drebs) control the expression of many stress-inducible genes in arabidopsis. a cdna clone, designated ghdreb1, was isolated from cotton (gossypium hirsutum) by cdna library screening. northern blot analysis indicated that mrna accumulation of ghdreb1 was induced by low temperatures and salt stress, but was not induced by abscisic acid (aba) or drought stress in cotton seedlings. transgenic to ... | 2007 | 17803642 |
| transgenic bacillus thuringiensis (bt) cotton (gossypium hirsutum) allomone response to cotton aphid, aphis gossypii, in a closed-dynamics co(2) chamber (cdcc). | allocation of allomones of transgenic bacillus thuringiensis gossypium hirsutum (bt cotton) (cv. gk-12) and non-bt-transgenic cotton (cv. simian-3) grown in elevated co(2) in response to infestation by cotton aphid, aphis gossypii glover, was studied in a closed-dynamics co(2) chamber. significant increases in foliar condensed tannin and carbon/nitrogen ratio for gk-12 and simian-3 were observed in elevated co(2) relative to ambient co(2,) as partially supported by the carbon nutrient balance hy ... | 2007 | 17805479 |
| toxicity of seven foliar insecticides to four insect parasitoids attacking citrus and cotton pests. | laboratory studies were carried out to compare the toxicity of seven foliar insecticides to four species of adult beneficial insects representing two families of hymenoptera: aphelinidae (aphytis melinus debach, eretmocerus eremicus rose & zolnerowich, and encarsiaformosa gahan) and mymaridae (gonatocerus ashmeadi girault) that attack california red scale, aonidiella aurantii (maskell); sweetpotato whitefly, bemisia tabaci (gennadius) (both e. eremicus and e. formosa); and glassy-winged sharpsho ... | 2007 | 17849851 |
| effects of proportion and configuration of bacillus thuringiensis cotton on pest abundance, damage, and yield. | bacillus thuringiensis (bt) transgenic cotton, gossypium hirsutum l., kills several economically important pests, reducing injury and increasing yields. refuges of non-bt cotton are currently planted with bt cotton in different designs to slow pest resistance evolution. to compare the effects of differences in bt/non-bt plant heterogeneity found in different refuge designs on square (flower bud) damage, abscissions, sap-feeding herbivore densities, and yield in cotton, four types of 24-row cotto ... | 2007 | 17849898 |
| a proteinaceous elicitor sm1 from the beneficial fungus trichoderma virens is required for induced systemic resistance in maize. | we have previously shown that the beneficial filamentous fungus trichoderma virens secretes the highly effective hydrophobin-like elicitor sm1 that induces systemic disease resistance in the dicot cotton (gossypium hirsutum). in this study we tested whether colonization of roots by t. virens can induce systemic protection against a foliar pathogen in the monocot maize (zea mays), and we further demonstrated the importance of sm1 during maize-fungal interactions using a functional genomics approa ... | 2007 | 17885089 |
| establishment of azotobacter on plant roots: chemotactic response, development and analysis of root exudates of cotton (gossypium hirsutum l.) and wheat (triticum aestivum l.). | biofertilizers contribute in n(2) fixation, p solubilization, phytohormone production and thus enhance plant growth. beneficial plant-microbe interactions and the stability and effectiveness of biofertilizer depend upon the establishment of bacterial strains in the rhizosphere of the plant. this interaction depends upon many factors, one of them being plant exudates. root exudates are composed of small organic molecules like carbonic acids, amino acids or sugars etc., which are released into the ... | 2007 | 17910096 |
| the american cotton rat: a novel model for pulmonary tuberculosis. | several animal models are used to study mycobacterium tuberculosis (mtb) infections, but none is a fully ideal model of human disease. the american cotton rat is an excellent model for the study of several human viral and bacterial respiratory infectious diseases, but until now has not been reported to be a model with mtb infection. preliminary experiments were designed in which two species of cotton rats (sigmodon hispidus and sigmodon fulviventer) received respiratory challenges with m. tuberc ... | 2007 | 16973421 |
| designing and transgenic expression of melanin gene in tobacco trichome and cotton fiber. | in streptomyces antibioticus, there are two genes tyra and orf438 required for the melanin biogenesis. to investigate whether expression of these two genes in cotton can change cotton fiber colour, we modified the tyra and orf438 genes to make their codon usage closer to the codon preference of cotton fiber genes. the resulting versions of these two genes were referred to as dtyra and dorf438, respectively. vacuolar targeting signals were also added to their ends. under the cotton fiber specific ... | 2007 | 17006798 |
| common subtropical and tropical nonpollen food sources of the boll weevil (coleoptera: curculionidae). | it is known that substantial boll weevil, anthonomus grandis grandis boheman, individuals can survive mild subtropical winters in some habitats, such as citrus orchards. our study shows that endocarp of the fruit from prickly pear cactus, opuntia engelmannii salm-dyck ex. engel.; orange, citrus sinensis l. osbeck.; and grapefruit, citrus paradisi macfad., can sustain newly emerged adult boll weevils for >5 mo, which is the duration of the cotton-free season in the subtropical lower rio grande va ... | 2007 | 17349122 |
| genetics of resistance to cotton leaf curl disease in gossypium hirsutum l. under field conditions. | one hundred and forty two cotton germplasm lines were screened for cotton leaf curl virus symptoms in field evaluations during 2003, 2004, and 2005. fifty cross combinations involving 30 of these lines classified resistant or susceptible were used for inheritance study of the disease. all the f(1) plants of crosses involving resistant x resistant, resistant x susceptible, and susceptible x resistant parents were resistant, indicating dominant expression of the disease resistance and there were n ... | 2007 | 17159229 |
| role of pantoea agglomerans in opportunistic bacterial seed and boll rot of cotton (gossypium hirsutum) grown in the field. | to investigate the aetiology of seed and boll rot of cotton grown in south carolina (sc). | 2007 | 17184328 |
| cross-resistance responses of crylac-selected heliothis virescens (lepidoptera: noctuidae) to the bacillus thuringiensis protein vip3a. | one susceptible and three cry1ac-resistant strains of tobacco budworm, heliothis virescens (f.) (lepidoptera: noctuidae), were used in laboratory studies to determine the level of cross-resistance between the bacillus thuringiensis (berliner) toxins cry1ac and vip3a by using concentration-mortality and leaf tissue experiments. concentration-mortality data demonstrated that the three cry1ac-resistant h. virescens strains, yhd2, kcbhyb, and cxc, were at least 215- to 316-fold resistant to cry1ac c ... | 2007 | 17370826 |
| a polymerase chain reaction screen of field populations of heliothis virescens for a retrotransposon insertion conferring resistance to bacillus thuringiensis toxin. | the evolution of pest resistance to transgenic crop plants producing insecticidal toxins from bacillus thuringiensis (bt) berliner poses a continuing threat to their sustainable use in agriculture. one component of the u.s.-wide resistance management plan for bt cotton, gossypium hirsutum l., involves monitoring the frequency of resistance alleles in field populations. however, existing methods are expensive and may not detect recessive resistance alleles until their frequencies are too high for ... | 2007 | 17370827 |
| molecular cloning and characterization of crmdr1, a novel mdr-type abc transporter gene from catharanthus roseus. | a novel gene encoding a mdr-like abc transporter protein was cloned from catharanthus roseus, a medicinal plant with more than 120 kinds of secondary metabolites, through rapid amplification of cdna ends (race). this gene (named as crmdr1; genbank accession no.: dq660356) had a total length of 4395 bp with an open reading frame of 3801 bp, and encoded a predicted polypeptide of 1266 amino acids with a molecular weight of 137.1 kda. the crmdr1 protein shared 59.8, 62.5, 60.0 and 58.2% identity wi ... | 2007 | 17541839 |
| cotton ghkch2, a plant-specific kinesin, is low-affinitive and nucleotide-independent as binding to microtubule. | kinesin is an atp-driven microtubule motor protein that plays important roles in control of microtubule dynamics, intracellular transport, cell division and signal transduction. the kinesin superfamily is composed of numerous members that are classified into 14 subfamilies. animal kinesins have been well characterized. in contrast, plant kinesins have not yet to be characterized adequately. here, a novel plant-specific kinesin gene, ghkch2, has been cloned from cotton (gossypium hirsutum) fibers ... | 2007 | 17927906 |
| simultaneous growth and emission measurements demonstrate an interactive control of methanol release by leaf expansion and stomata. | emission from plants is a major source of atmospheric methanol. growing tissues contribute most to plant-generated methanol in the atmosphere, but there is still controversy over biological and physico-chemical controls of methanol emission. methanol as a water-soluble compound is thought to be strongly controlled by gas-phase diffusion (stomatal conductance), but growth rate can follow a different diurnal rhythm from that of stomatal conductance, and the extent to which the emission control is ... | 2007 | 17374874 |
| feeding and oviposition preference of phyllophaga cuyabana (moser) (coleoptera: melolonthidae) on several crops. | laboratory and greenhouse experiments were carried out to study food and oviposition preference by phyllophaga cuyabana (moser) on different plant species as cajanus cajan l. (pigeon pea), crotalaria juncea l. (sun hemp), crotalaria spectabilis roth (showy crotalaria), crotalaria ochroleuca g. don (slenderleaf rattlebox), glycine max [l.] merrill (soybean), gossypium hirsutum l. (cotton), helianthus annuus l. (sunflower), stizolobium aterrimum [mucuna aterrima] piper tracey (velvetbean) and zea ... | 2007 | 18060303 |
| comparison of direct and indirect sampling methods for tarnished plant bug (hemiptera: miridae) in flowering cotton. | a complex of hemipterans, especially the tarnished plant bug, lygus lineolaris (palisot de beauvois) (hemiptera: miridae), has become a major target of insecticides in flowering cotton, gossypium hirsutum l., in the mid-southern united states. sampling protocols for this complex during this period of cotton development are poorly established, resulting in uncertainty about when infestations warrant treatment. nine direct and indirect sampling methods were evaluated for bias, precision, and effic ... | 2007 | 18232411 |
| expression partitioning between genes duplicated by polyploidy under abiotic stress and during organ development. | allopolyploidy has been a prominent mode of speciation and a recurrent process during plant evolution and has contributed greatly to the large number of duplicated genes in plant genomes [1-4]. polyploidy often leads to changes in genome organization and gene expression [5-9]. the expression of genes that are duplicated by polyploidy (termed homeologs) can be partitioned between the duplicates so that one copy is expressed and functions only in some organs and the other copy is expressed only in ... | 2007 | 17825563 |
| a strategy for designing multi-taxa specific reference gene systems. example of application--ppi phosphofructokinase (ppi-ppf) used for the detection and quantification of three taxa: maize (zea mays), cotton (gossypium hirsutum) and rice (oryza sativa). | in the first part of the paper, we report the description of a new strategy for the development of a plant reference gene system that can be used for genetically modified organism (gmo) analysis. on the basis of in silico research for candidate genes, the design of degenerate primers allowed the obtention of genomic sequences of the selected gene ppi-phosphofructokinase ( ppi-ppf) for nine taxa in which gmos have been developed. the comparison and the analysis of inter- and intraspecies sequence ... | 2007 | 17824661 |
| the induction of microsomal nadph:cytochrome p450 and nadh:cytochrome b(5) reductases by long-term salt treatment of cotton (gossypium hirsutum l.) and bean (phaseolus vulgaris l.) plants. | we studied the effect of salinity on the activity of microsomal nadph:cytochrome p450 reductase (cpr, ec 1.6.2.4) and nadh:ferricytochrome b(5) oxidoreductase (b5r, ec 1.6.2.2) in two dicotyledonous plant species differing in their sensitivity to salt, cotton (gossypium hirsutum l. cv ogosta) and common bean (phaseolus vulgaris l. cv dobrujanski 7). a significant inhibition of fresh weight of salt-treated bean plants was observed, while cotton was affected to a much lesser degree. nacl applicati ... | 2007 | 17761430 |
| comparative characteristics and gene action in three petal-spotted mutants of gossypium hirsutum. | 2007 | 17656854 | |
| meiosis in a triploid hybrid of gossypium: high frequency of secondary bipolar spindles at metaphase ii. | studies on meiosis in pollen mother cells (pmcs) of a triploid interspecific hybrid (3x = 39 chromosomes, aad) between tetraploid gossypium hirsutum (4n = 2x = 52,aadd) and diploid g. arboreum (2n = 2x = 26,aa) are reported. during meiotic metaphase i, 13 aa bivalents and 13 d univalents are expected in the hybrid. however, only 28% of the pmcs had this expected configuration. the rest of the pmcs had between 8 and 12 bivalents and between 12 and 17 univalents. univalents lagged at anaphase i, a ... | 2007 | 17656848 |
| laser capture microdissection and cdna microarrays used to generate gene expression profiles of the rapidly expanding fibre initial cells on the surface of cotton ovules. | cotton (gossypium hirsutum l.) fibre initial cells undergo a rapid cellular re-programming around anthesis to form the long cellulose fibres prized for textile manufacture. on the day of anthesis the cotton fibre initial cells balloon out from the ovule surface and so are clearly distinguished from adjacent epidermal pavement cells. to enhance our understanding of the molecular processes that determine which cells become fibres and why adjacent epidermal cells remain in a different developmental ... | 2007 | 17636323 |
| development of one set of chromosome-specific microsatellite-containing bacs and their physical mapping in gossypium hirsutum l. | fluorescence in situ hybridization (fish), using bacterial artificial chromosome (bac) clone as probe, is a reliable cytological technique for chromosome identification. it has been used in many plants, especially in those containing numerous small chromosomes. we previously developed eight chromosome-specific bac clones from tetraploid cotton, which were used as excellent cytological markers for chromosomes identification. here, we isolated the other chromosome-specific bac clones to make a com ... | 2007 | 17632699 |
| analysis of genetic effects of nuclear-cytoplasmic interaction on quantitative traits: genetic model for diploid plants. | a genetic model was proposed for simultaneously analyzing genetic effects of nuclear, cytoplasm, and nuclear-cytoplasmic interaction (nci) as well as their genotype by environment (ge) interaction for quantitative traits of diploid plants. in the model, the nci effects were further partitioned into additive and dominance nuclear-cytoplasmic interaction components. mixed linear model approaches were used for statistical analysis. on the basis of diallel cross designs, monte carlo simulations show ... | 2007 | 17601616 |
| analysis of differentially expressed genes in genic male sterility cotton (gossypium hirsutum l.) using cdna-aflp. | cdna amplified fragment length polymorphism (cdna-aflp) analysis was used to investigate the differentially expressed genes between sterile and fertile plants of ms5ms6 double-recessive genic male sterility (gms) two-type line cotton (gossypium hirsutum l.) at different stages, i.e., sporogenous cell stage, pollen mother cell (pmc) stage, and pollen grain stage. seventeen differentially expressed fragments were identified. functional analysis indicated that their corresponding genes may particip ... | 2007 | 17601613 |
| ghdet2, a steroid 5alpha-reductase, plays an important role in cotton fiber cell initiation and elongation. | cotton (gossypium hirsutum l.) fibers, one of the most important natural raw materials for textile industry, are highly elongated trichomes from epidermal cells of cotton ovules. det2, an arabidopsis steroid 5d-reductase, is considered to catalyze a major rate-limiting in brassinosteroid (br) biosynthesis. to understand the role of brs in cotton fiber development, ghdet2, which putatively encodes a steroid 5alpha-reductase by sequence comparison, was cloned from developing fiber cells. in vitro ... | 2007 | 17565582 |
| [estimation of the efficiency of seed irradiation by thermal neutrons for inducing chromosomal aberrations in m1 of cotton gossypium hirsutum l]. | exposure of cotton seeds to thermal neutrons at doses of 15, 25, and 35 gy was shown to induce many altered plants, including sterile and chimeric ones. most of these phenotypic changes were shown to result from novel genomic, chromosomal, and desynaptic mutations. the presence of these mutations in the karyotype of m, plants often decreased meiotic index and pollen fertility. in translocation forms, the decrease in pollen fertility was caused by the prevalence of quadrivalents in form of rings ... | 2007 | 17555126 |
| [the inheritance of an ultra-dwarf plant mutant from upland cotton]. | the inheritance of an ultra-dwarf plant mutant from upland cotton (gossypium hirsutum l.) was studied, which showed that the mutant was controlled by single recessive quality gene. this gene was denominated as du tentatively. no similar mutant has been found in upland cotton. the mutation could not normally flower and produce bolls under natural conditions, and its mature height was only 10.5 cm. when treated with exogenous ga3, it could normally flower and boll, and plant height could reach 57. ... | 2007 | 17548311 |
| quantitative analysis of cotton (gossypium hirsutum) lint trash by fluorescence spectroscopy. | the presence of cotton plant botanical components, or trash, embedded in lint subsequent to harvesting and ginning is an important criterion in the classification of baled cotton by the u.s. department of agriculture agricultural marketing service. the trash particles may be reduced in size to the point that specific trash types are not identifiable by image or gravimetric analysis, and it is desirable to quantify different trash types so that processing lines may be optimized for removal of the ... | 2007 | 17536818 |
| analysis of gene expression in cotton fiber initials. | cotton (gossypium hirsutum l.) fibers are trichomes that initiate from the ovule epidermis. little is known about the developmental pathway causing fiber to differentiate from ovular epidermal cells even though limits on the number of cells that differentiate into fiber will limit yield. | 2007 | 17506877 |
| molecular characterization of four adf genes differentially expressed in cotton. | actin depolymerizing factor (adf), highly conserved in all eukaryotic cells, is a low molecular mass of actin-binding protein, which plays a key role in modulating the polymerizing and depolymerizing of the actin filaments. four cdnas (designated ghadf2, ghadf3, ghadf4, and ghadf5, respectively) encoding adf proteins were isolated from cotton (gossypium hirsutum) fiber cdna library. ghadf2 cdna is 705 bp in length and deduces a protein with 139 amino acids. ghadf3 cdna is 819 bp in length and en ... | 2007 | 17498633 |
| the role of induced mutation in conversion of photoperiod dependence in cotton. | wild cotton germplasm resources are largely underutilized because of photoperiod-dependent flowering of "exotic" cottons. the objectives of this work were to explore the genome-wide effect of induced mutation in photoperiod-converted induced cotton mutants, estimating the genetic change between mutant and wild-type cottons using simple sequence repeats (ssrs) as well as understand the pattern of ssr mutation in induced mutagenesis. three groups of photoperiod-converted radiomutants ((32)p) inclu ... | 2007 | 17406024 |
| changes in homogalacturonans and enzymes degrading them during cotton cotyledon expansion. | changes in homogalacturonans (hgs) and enzymes degrading them have been investigated during cotton (gossypium hirsutum l.) cotyledon expansion. using an in vivo assay for pectin-degrading enzymes that involves fluorescent labeled oligomers of gala as substrate and capillary electrophoresis for product analysis, we found that endo- and exo-polygalacturonases are present in the cotyledon extracellular spaces, and there are dramatic changes in the levels of both activities as the cotyledons change ... | 2007 | 17395221 |
| effects of crop rotation, tillage, and fertilizer applications on sorghum head insects. | rotations, tillage, and fertilizer treatments can affect yield, costs, and profitability in sorghum, sorghum bicolor (l.) moench, depending on their effects on pests. rotation or planting different crops reduces soil erosion and pests that build up when a field is planted to the same crop each year. minimum tillage reduces the number of trips over a field, lessening soil compaction and reducing costs. we examined the effects of fertilizer, tillage, and rotation with cotton, gossypium hirsutum l. ... | 2007 | 17370814 |
| detection and identification of rhamnogalacturonan lyase activity in intercellular spaces of expanding cotton cotyledons. | rhamnogalacturonan lyase (rg lyase) activity has been detected and its relative activity measured in vivo during the expansion of cotton (gossypium hirsutum l.) cotyledons. rhamnogalacturonan (rg) oligomers labeled with a fluorescent tag were injected into the intercellular spaces of cotton cotyledons and, after incubation, the digested substrate was rinsed out. enzyme digestion products were detected and identified by capillary zone electrophoresis. rhamnogalacturonan lyase products were identi ... | 2007 | 17346264 |
| a quantitative method for the determination of cyclopropenoid fatty acids in cottonseed, cottonseed meal, and cottonseed oil (gossypium hirsutum) by high-performance liquid chromatography. | cyclopropenoid fatty acids (cpfas), found in cottonseed, have been shown to have detrimental health effects to susceptible livestock. previous quantitative analytical methods for the determination of cpfas expressed these acids in terms of their relative abundance with respect to other fatty acids in the oil, necessitating the concurrent analysis of other fatty acids. the proposed analytical method describes the quantitation of three relevant cpfas for cotton (malvalic acid, sterculic acid, and ... | 2007 | 17323967 |
| transgenic cotton over-producing spinach sucrose phosphate synthase showed enhanced leaf sucrose synthesis and improved fiber quality under controlled environmental conditions. | prior data indicated that enhanced availability of sucrose, a major product of photosynthesis in source leaves and the carbon source for secondary wall cellulose synthesis in fiber sinks, might improve fiber quality under abiotic stress conditions. to test this hypothesis, a family of transgenic cotton plants (gossypium hirsutum cv. coker 312 elite) was produced that over-expressed spinach sucrose-phosphate synthase (sps) because of its role in regulation of sucrose synthesis in photosynthetic a ... | 2007 | 17287885 |
| rapid development of enhanced atrazine degradation in a dundee silt loam soil under continuous corn and in rotation with cotton. | mississippi delta cotton (gossypium hirsutum l.) production in rotation with corn (zea mays l.) was evaluated in field experiments from 2000 to 2005 at stoneville, mississippi. plots maintained under minimum tillage were established in 2000 on a dundee silt loam with treatments including continuous cotton or corn and alternate cotton-corn rotations. mineralization and dissipation of 14c [ring]-labeled atrazine were evaluated in the laboratory on soils collected prior to herbicide application in ... | 2007 | 17263485 |
| molecular cloning and expression analysis of a ranbp2 zinc finger protein gene in upland cotton (gossypium hirsutum l.). | gossypol is an important resistant substance of gossypium, and its storage organ is pigment gland. although, the relationship between gossypol and pigment gland has been studied for a long time, the development mechanism of pigment gland has not been revealed up to now in molecular perspective. on the basis of differentially expressed cdnas fragments at the stage of the cotton gland development using suppression subtractive hybridization (ssh), the complete cdna sequence of a novel ranbp2 zinc f ... | 2007 | 17223018 |
| combining ability and heterosis for earliness characters in line x tester population of gossypium hirsutum l. | the objective of this study was to estimate the general combining ability of the parents and specific combining ability of hybrids for earliness traits for line selection. inheritance and interrelationships of earliness characters were evaluated in a line x tester design. three intermediate-early-maturing female (lines) which are grown regionally and four early-maturing males (testers) cotton varieties were crossed in 2003. the twelve f(1) and seven parents were planted randomized block design w ... | 2007 | 18031352 |
| influence of nezara viridula feeding on cotton yield, fiber quality, and seed germination. | the influence of southern green stink bug, nezara viridula (l.), adults (males and females) and fourth to fifth instars on cotton, gossypium hirsutum l., boll abscission, seedcotton yield, fiber quality, and seed viability was evaluated in field studies conducted during 2004 and 2005. cotton bolls representing several age classes ranging from 0-600 heat units were individually infested with a specific gender or life stage of southern green stink bug. adults and nymphs induced abscission of bolls ... | 2007 | 17972633 |
| molecular characterization of cotton 14-3-3l gene preferentially expressed during fiber elongation. | the 14-3-3 protein, highly conserved in all eukaryotic cells, is an important regulatory protein. it plays an important role in the growth, amplification, apoptosis, signal transduction, and other crucial life activities of cells. a cdna encoding a putative 14-3-3 protein was isolated from cotton fiber cdna library. the cdna, designated as gh14-3-3l (gossypium hirsutum 14-3-3-like), is 1,029 bp in length (including a 762 bp long open reading frame and 5'-/3'-untranslated regions) and deduced a p ... | 2007 | 17469787 |
| microcolinearity and genome evolution in the adha region of diploid and polyploid cotton (gossypium). | genome sizes vary by several orders of magnitude, driven by mechanisms such as illegitimate recombination and transposable element proliferation. prior analysis of the cesa region in two cotton genomes that diverged 5-10 million years ago (ma), and acquired a twofold difference in genome size, revealed extensive local conservation of genic and intergenic regions, with no evidence of the global genome size difference. the present study extends the comparison to include bac sequences surrounding t ... | 2007 | 17461788 |
| integrative mapping of gossypium hirsutum l. by meiotic fluorescent in situ hybridization of a tandemly repetitive sequence (b77). | we determined the relative positions of the tandem-repeat molecular cytogenetic marker b77, translocation breakpoints, and telosome arms in gossypium hirsutum cytogenetic stocks by fluorescence in situ hybridization (fish) analysis of meiotic quadrivalents in 16 single and 2 double translocation heterozygotes and five monotelodisomics. results delimited the b77 fish locus to the right arm of the d-subgenome chromosome 14 (14r) and the short arm (14sh), respectively. by equating 14r with 14sh and ... | 2007 | 17409065 |
| computational identification of micrornas and their targets in gossypium hirsutum expressed sequence tags. | micrornas (mirnas) are a class of non-coding rnas that regulate gene post-transcriptional expression in animals and plants. comparatively genomic computational methods have been developed to predict new mirnas in worms, humans, and arabidopsis. here we present an est (expressed sequence tags)--and gss (genomic survey sequences)-based combined approach for the detection of novel mirnas in gossypium hirsutum. this was initiated by using previously known mirna sequences from arabidopsis, rice and o ... | 2007 | 17408884 |