Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| a view of plant dehydrins using antibodies specific to the carboxy terminal peptide. | dehydrins are characterized by the consensus kikeklpg amino acid sequence found near the carboxy terminus, and usually repeated from one to many times within the protein. a synthetic peptide containing this consensus sequence was used to produce specific antibodies that recognize dehydrins in a wide range of plants. this range covered two families of monocots, viz. gramineae (hordeum vulgare l., triticum aestivum l., zea mays l., oryza sativa l.) and liliaceae (allium sativa l.), and five famili ... | 1993 | 7693020 |
| toxicity of cotton phytoalexins to zoopathogenic fungi. | the sesquiterpenoid phytoalexins desoxyhemigossypol, desoxymethoxyhemigossypol, and hemigossypolone formed in cotton (gossypium hirsutum and g. barbadense) stem xylem infected with verticillium dahliae were shown to be highly toxic to zoopathogenic fungi. this appears to be the first study of the toxicity of terpenoid phytoalexins to zoopathogenic fungi. the toxicities of the phytoalexins expressed as mic (micrograms ml-1) values were 8 to 128 against four isolates of candida albicans and one is ... | 1993 | 8167949 |
| permanent gases inside healthy and microbially infected cotton fruit during development. | permanent gases inside developing cotton fruit (gossypium hirsutum l.) were, by weight, 46% nitrogen, 29% oxygen, 4% argon and 20% carbon dioxide, whereas plant canopy air assayed at 73% nitrogen, 25% oxygen, 2% argon and 0.3% carbon dioxide. light exposure, fruit age, and mild infection (erwinia) had no compositional effect but aggressive infection (aspergillus) raised carbon dioxide content to 31% by weight and correspondingly lowered oxygen to 17%. respiration with oxygen replenishment except ... | 1993 | 8466505 |
| water relation alterations observed during hypersensitive reaction induced by bacteria. | upon exposure to pathogenic bacteria, resistant and nonhost plants undergo a hypersensitive reaction (hr) that is expressed as rapid plant cell death. if sufficient concentrations of these bacteria are inoculated to such plant tissue, then that portion of the tissue rapidly collapses and becomes necrotic. as the tissue collapses the water relations of inoculated tissues become markedly disturbed. we measured a decline in the relative water content (rwc) in the leaf-like cotyledons of cotton (gos ... | 1993 | 12232016 |
| damage functions and population changes of hoplolaimus columbus on cotton and soybean. | damage functions and reproductive curves were determined for hoplolaimus columbus on cotton cv. deltapine 90 and soybean cv. gordon over 2 years in field plots in georgia. maximum potential yield suppressions of 18% on cotton and 48% on soybean were predicted with respect to increasing pi. similar functions indicated yield suppressions of 38% on cotton and 30% on soybean with respect to increasing midseason nematode densities (pm). maximum pf predicted by reproductive curves were 123 and 474/100 ... | 1993 | 19279792 |
| meloidogyne incognita and rotylenchulus reniformis and associated soil textures from some cotton production areas of texas. | the incidence of meloidogyne incognita and rotylenchulus reniformis on cotton was determined in 1989-92 from 1,089 soil samples collected from 31 counties that account for nearly 60% of the 2.2 million hectares planted to cotton in texas. meloidogyne incognita was commonly found in the southern high plains and brazos river valley regions of texas (57% and 34%, respectively, of samples) but was found in less than 8% of samples from the central blacklands, coastal bend, low plains, or the upper gu ... | 1993 | 19279860 |
| effects of 1,3-dicliloropropene for meloidogyne incognita management on cotton produced under furrow irrigation. | field trials were conducted during 1990 to evaluate the effects of preplant soil fumigation with 1,3-dichloropropene (1,3-d) on yield and fiber quality of furrow-irrigated cotton cultivars subjected to high population densities of meloidogyne incognita. we measured the responses of eight upland cotton cultivars with different levels of root-knot nematode resistance and compared the responses of upland and pima cottons. reductions in lint weight ranged from 10 to 52% among cultivars grown in soil ... | 1993 | 19279835 |
| caffeic acid and glycerol are constituents of the suberin layers in green cotton fibres. | the fibres of the green-lint mutant (lg) of cotton (gossypium hirsutum l.) are suberized and contain a large proportion of wax. the unidentified components of the wax were separated into a colourless fluorescent fraction and a yellow pigmented fraction. using ultraviolet spectroscopy and nuclear-magneticresonance ((1)h-nmr) spectroscopy, esterified trans-caffeic acid was identified as the only phenolic component in the colourless fraction. this fraction was further purified and was shown to cont ... | 1993 | 24178505 |
| lipids, proteins, and structure of seed oil bodies from diverse species. | oil bodies isolated from the mature seeds of rape (brassica napus l.), mustard (brassica juncea l.), cotton (gossypium hirsutum l.), flax (linus usitatis simum), maize (zea mays l.), peanut (arachis hypogaea l.), and sesame (sesamum indicum l.) had average diameters that were different but within a narrow range (0.6-2.0 [mu]m), as measured from electron micrographs of serial sections. their contents of triacylglycerols (tag), phospholipids, and proteins (oleosins) were correlated with their size ... | 1993 | 12231682 |
| identification of a homeologous chromosome pair by in situ dna hybridization to ribosomal rna loci in meiotic chromosomes of cotton (gossypium hirsutum). | in situ dna hybridization with 18s-28s and 5s ribosomal dna probes was used to map 18s-28s nucleolar organizers and tandem 5s repeats to meiotic chromosomes of cotton (gossypium hirsutum l.). mapping was performed by correlating hybridization sites to particular positions in translocation quadrivalents. arm assignment required translocation quadrivalents with at least one interstitial chiasma and sufficient distance between the hybridization site and the centromere. we had previously localized a ... | 1993 | 18470044 |
| catalytic properties of a newly discovered acyltransferase that synthesizes n-acylphosphatidylethanolamine in cottonseed (gossypium hirsutum l.) microsomes. | we recently demonstrated that cotyledons of cotton (gossypium hirsutum l.) seedlings synthesize n-acylphosphatidylethanolamine (nape), an unusual acylated derivative of phosphatidylethanolamine (pe), during postgerminative growth (k.d. chapman and t.s. moore [1993] arch biochem biophys 301: 21-33). here, we report the discovery of an acyltransferase enzyme, fatty acid: diacylphosphatidylethanolamine n-acyltransferase (designated nape synthase), that synthesizes nape from pe and free fatty acids ... | 1993 | 12231864 |
| inhibitions of cotton seedling growth by volatile ketones emitted by cover crop residues. | low-input, sustainable agriculture (lisa) systems can include soil incorporation of residues of weeds and legume cover crop species. however, both seed germination and seedling growth of crops, including cotton (gossypium hirsutum l.), are inhibited by volatile organic compounds emitted by these residues during decomposition. a cotton seedling-growth assay was used to demonstrate that c4 through c9 ketones released by decomposing legume residues significantly inhibited root and shoot elongation, ... | 1993 | 24249128 |
| vacuolar h(+)-atpase 69-kilodalton catalytic subunit cdna from developing cotton (gossypium hirsutum) ovules. | 1993 | 8108515 | |
| copia-like retrotransposable element evolution in diploid and polyploid cotton (gossypium l.). | copia-like retrotransposable elements were identified in allotetraploid cotton, gossypium hirsutum, and two species representing its diploid progenitors, g. herbaceum and g. raimondii. these elements are present in high copy number in all three species. because the two diploid genomic groups have been isolated on opposite sides of the world for 6-11 million years, horizontal transfer of elements between these species is highly unlikely. elements were intensively sampled to generate a model of co ... | 1993 | 7685393 |
| [beta]-glucan synthesis in the cotton fiber (iii. identification of udp-glucose-binding subunits of [beta]-glucan synthases by photoaffinity labeling with [[beta]-32p]5[prime]-n3-udp-glucose. | using differential product entrapment and photolabeling under specifying conditions, we identified a 37-kd polypeptide as the best candidate among the udp-glucose-binding polypeptides for the catalytic subunit of cotton (gossypium hirsutum) cellulose synthase. this polypeptide is enriched by entrapment under conditions favoring [beta]-1,4-glucan synthesis, and it is magnesium dependent and sensitive to unlabeled udp-glucose. a 52-kd polypeptide was identified as the most likely candidate for the ... | 1993 | 12231766 |
| [beta]-glucan synthesis in the cotton fiber (ii. regulation and kinetic properties of [beta]-glucan synthases. | the regulation and kinetic properties of cellulose synthase as well as [beta]-1,3-glucan synthase have been studied. the cellulose was detected using acetic/nitric acid insolubility as an indicator of cellulose (this product contained only [beta]-1,4-linked glucans; k. okuda, l. li, k. kudlicka, s. kuga, r.m. brown, jr. [1993] plant physiol 101: 1131-1142). these studies reveal that (a) [beta]-1,3-glucan synthesis is enhanced up to 31-fold by cellobiose with a ka of 1.16 mm; (b) cellulose synthe ... | 1993 | 12231765 |
| [beta]-glucan synthesis in the cotton fiber (i. identification of [beta]-1,4- and [beta]-1,3-glucans synthesized in vitro). | in vitro [beta]-glucan products were synthesized by digitonin-solubilized enzyme preparations from plasma membrane-enriched fractions of cotton (gossypium hirsutum) fiber cells. the reaction mixture favoring [beta]-1,4-glucan synthesis included the following effectors: mg2+, ca2+, cellobiose, cyclic-3[prime]:5[prime]-gmp, and digitonin. the ethanol insoluble fraction from this reaction contained [beta]-1,4-glucan and [beta]-1,3-glucan in an approximate ratio of 25:69. approximately 16% of the [b ... | 1993 | 12231764 |
| a method for the isolation of nuclear dna from cotton (gossypium) leaves. | cotton leaves contain high levels of polyphenolic compounds that irreversibly interact with proteins and nucleic acids during dna isolation. a procedure to isolate nuclear dna from cotton (gossypium hirsutum l.) has been developed. the method is based on the rapid initial isolation of nuclei in a glucose medium designed to stabilize nuclear structure and composition while preventing covalent interactions with polyphenolics. the resulting dna is high in yield and purity and is suitable for southe ... | 1993 | 8098189 |
| involvement of abscisic acid in ethylene-induced cotyledon abscission in cotton seedlings. | cotton (gossypium hirsutum l. cv lg102) seedlings raised from seeds exposed to 100 [mu]m norflurazon (nfz) during imbibition contained reduced levels of free abscisic acid (aba) and were visibly achlorophyllous. exposure of untreated cotton seedlings to ethylene concentrations >1 [mu]l/l for 24 h resulted in cotyledon abscission. in contrast, exposure of nfz-treated seedlings to concentrations of ethylene [less than or equal to]50 [mu]l/l elicited no cotyledon abscission. application of aba, an ... | 1993 | 12231720 |
| cotton (gossypium hirsutum) matp6 and matp7 oleosin genes. | 1993 | 8278515 | |
| correlation between the circadian rhythm of resistance to extreme temperatures and changes in fatty acid composition in cotton seedlings. | fluctuations in fatty acid composition were examined in cotton (gossypium hirsutum l. cv deltapine 50) leaves during light-dark cycles of 12:12 h and under continuous light and were correlated to the rhythmic changes in chilling (5[deg]c) resistance (cr) and heat (53[deg]c) resistance (hr). the chilling-resistant and chilling-sensitive phases developed in the dark or the light period, respectively, and this rhythm persisted under continuous light for three cycles. the heat-resistant phase develo ... | 1993 | 12231662 |
| herbivore-induced volatile emissions from cotton (gossypium hirsutum l.) seedlings. | the effect of herbivory on the composition of the volatile blends released by cotton seedlings was investigated by collecting volatiles from undamaged, freshly damaged (0-2 hr after initiation of feeding), and old damaged (16-19 hr after initiation of feeding) plants on which corn earworm caterpillars (helicoverpa zea boddie) were actively feeding. a blend of 22 compounds was consistently observed to be emitted by the old damaged plants with nine occurring either only in, or in significantly gre ... | 1994 | 24241975 |
| a modified hot borate method significantly enhances the yield of high-quality rna from cotton (gossypium hirsutum l.). | the isolation of biologically active rna from cotton (gossypium hirsutum l.) is difficult due to interference by high levels of endogenous phenolics, polysaccharides, and secondary metabolites. a modified hot borate procedure was developed to combat these cellular constituents during tissue homogenization, resulting in the quantitative recovery of rna suitable for hybridization analysis, in vitro translation, and cdna synthesis. the efficacy of several hot borate buffer adjuvants for the qualita ... | 1994 | 7535022 |
| effects of host plant,gossypium hirsutum l., on sexual attraction of cabbage looper moths,trichoplusia ni (hübner) (lepidoptera: noctuidae). | unmated female or male cabbage looper moths,trichoplusia ni (hübner), were attracted more often in a flight tunnel to a cage with moths of the opposite sex and a bouquet of cotton foliage. increased sexual attractiveness of females with plants may be a result of stimulation of pheromone release in response to plant odor, since more males were attracted when odor of cotton foliage was passed over females than when odor of females was passed over cotton foliage before venting into the flight tunne ... | 1994 | 24241928 |
| isolation and characterization of a cdna encoding ribosomal protein s16 from cotton (gossypium hirsutum l.). | 1994 | 7824648 | |
| ancient origin of the vacuolar h(+)-atpase 69-kilodalton catalytic subunit superfamily. | recently, two distinct cdna clones encoding the catalytic subunit of the vacuolar h(+)-atpase (v-atpase) were isolated from the allotetraploid cotton species gossypium hirsutum l. cv 'acala sj-2' (wilkins 1992, 1993). differences in the nucleotide sequence of these clones were used as molecular markers to explore the organization and structure of the v-atpase catalytic subunit genes in the a and d genomes of diploid and allotetraploid cotton species. nucleotide sequencing of polymerase chain rea ... | 1994 | 24177903 |
| isolation of multiple cdnas encoding the vacuolar h(+)-atpase subunit b from developing cotton (gossypium hirsutum l.) ovules. | 1994 | 7972522 | |
| effects of nacl on flows of n and mineral ions and on no3- reduction rate within whole plants of salt-sensitive bean and salt-tolerant cotton. | the effects of nacl on the transport rates of cations, no3-, and reduced n compounds between roots and shoot and on no3- assimilation rate were examined on plants of two species differing in their sensitivity to salinity, bean (phaseolus vulgare l. cv gabriella) and cotton (gossypium hirsutum l. cv akala). biomass production after 20 d in response to 50 and 100 mm nacl decreased by 48 and 59% in bean, but only 6 and 14% in cotton. the comparison of the flow patterns obtained for control and nacl ... | 1994 | 12232296 |
| changes in the accumulation of [alpha]- and [beta]-tubulin isotypes during cotton fiber development. | the expression of [alpha]- and [beta]-tubulin proteins in developing fibers and several other tissues of cotton (gossypium hirsutum, cv texas marker 1) have been analyzed by immunoblots of one- and two-dimensional gels utilizing anti-tubulin antibodies as probes. as a percentage of total protein, fibers had greater amounts of tubulin than did hypocotyls, roots, leaves, or cotyledons. both [alpha]- and [beta]-tubulin, having apparent molecular masses of approximately 50 kd and isoelectric points ... | 1994 | 12232289 |
| isolation and characterization of a cdna encoding ribosomal protein l41 from cotton (gossypium hirsutum l.). | 1994 | 7972506 | |
| the expression and anaerobic induction of alcohol dehydrogenase in cotton. | the alcohol dehydrogenase (adh) system in cotton is characterized, with an emphasis on the cultivated allotetraploid species gossypium hirsutum cv. siokra. a high level of adh activity is present in seed of siokra but quickly declines during germination. when exposed to anaerobic stress the level of adh activity can be induced several fold in both roots and shoots of seedlings. unlike maize and arabidopsis, adh activity can be anaerobically induced in mature green leaves. three major adh isozyme ... | 1994 | 7826315 |
| the effects of nacl on antioxidant enzyme activities in callus tissue of salt-tolerant and salt-sensitive cotton cultivars (gossypium hirsutum l.). | to determine nacl effects on callus growth and antioxidant activity, callus of a salt-tolerant and a salt-sensitive cultivar of cotton was grown on media amended with 0, 75, and 150 mm nacl. callus of the salt-tolerant cultivar, acala 1517-8 8, grown at 150 mm nacl, showed significant increases in superoxide dismutase, catalase, ascorbate peroxidase, peroxidase and glutathione reductase activities compared to callus tissue grown at 0 mm nacl. in contrast, callus tissue of the salt-sensitive cult ... | 1994 | 24194128 |
| protoplast-to-plant regeneration in cotton (gossypium hirsutum l. cv. coker 312) using feeder layers. | we report the regeneration of protoplasts isolated from two embryogenic cell lines of gossypium hirsutum l. cv. coker 312 initiated from hypocotylderived callus. protoplasts plated on cellulose nitrate filters and placed over feeder layers formed embryogenic callus from which plants were regenerated. plating efficiency up to 12.8% depended upon the cell line. addition of phytohormones to the protoplast medium had no stimulating effect on plating efficiency. the influence of feeder cells and cond ... | 1994 | 24193653 |
| effects of tropical rotation crops on meloidogyne arenaria population densities and vegetable yields in microplots. | the effects of 12 summer crop rotation treatments on population densities of meloidogyne arenaria race 1 and on yields of subsequent spring vegetable crops were determined in microplots. the crop sequence was: (i) rotation crops during summer 1991 ; (ii) cover crop of rye (secale cereale) during winter 1991-92; (iii) squash (cucurbita pepo) during spring 1992; (iv) rotation crops during summer 1992; (v) rye during winter 1992-93; (vi) eggplant (solanum melongena) during spring 1993. the 12 rotat ... | 1994 | 19279880 |
| interrelationships of rotylenchulus reniformis with rhizoctonia solani on cotton. | the interrelationships between reniform nematode (rotylenchulus reniformis) and the cotton (gossypium hirsutum) seedling blight fungus (rhizoctonia solani) were studied using three isolates of r. solani, two populations of r. reniformis at multiple inoculum levels, and the cotton cultivars dehapine 90 (dp 90) and dehapine 41 (dp 41). colonization of cotton hypocotyl tissue by r. solani resulted in increases (p </= 0.05) in nematode population densities in soil and in eggs recovered from the root ... | 1994 | 19279918 |
| influence of rhizoctonia solani on egg hatching and infectivity of rotylenchulus reniformis. | the effects of culture filtrates of rhizoctonia solani and root exudates of r. solani-infected cotton (gossypium hirsutum) seedlings on hatching of eggs and infectivity of females of rotylenchulus reniformis were evaluated in an attempt to account for the enhanced nematode reproduction observed in the presence of this fungus. crude filtrates of r. solani cultures growing over sterile, deionized distilled water did not affect egg hatching. exudates from roots of cotton seedlings increased hatchin ... | 1994 | 19279919 |
| rotations with coastal bermudagrass, cotton, and bahiagrass for management of meloidogyne arenaria and southern blight in peanut. | the efficacy of coastal bermudagrass (cynodon dactylon) as a rotation crop for controt of root-knot nematode (meloidogyne arenaria) in 'florunner' peanut (arachis hypogoea) was evaluated in a 3-year field trial. coastal bermudagrass-peanut rotation (cbp) was compared with peanut monoculture without nematicide (p - ) and peanut monoculture with aldicarb (p + ). the performance of cbp was also compared with 'pensacola' bahiagrass (paspalum notatum)-peanut (bp), and 'deltapine 90' cotton (gossypium ... | 1994 | 19279945 |
| plant-parasitic nematodes associated with cotton in florida. | a sampling of 15% of the cotton hectarage in each florida county was assayed for nematodes and soil particle components following the 1990 harvest. the distribution of juveniles of meloidogyne spp., which were found in 61% of the 178 fields sampled statewide, was not influenced by soil type. rotylenchulus reniformis was more prevalent in the heavier soils and occurred in 15% of the sampled fields. in fields with concomitant infestations (9% of the sampled fields), densities of root-knot juvenile ... | 1994 | 19279958 |
| simultaneous induction of postabscission and germination mrnas in cultured dicotyledonous embryos. | cloned mrnas identify three programs of gene expression in cotton (gossypium hirsutum l.) embryos that are associated with the maturation (reserve accumulation) stage, the postabscission stage, which is marked by expression of late-embryogenesis-abundant (lea) mrnas, and germination (broadly defined as including all events through early postgerminative growth). in order to test if the regulation of these programs is the same in other dicotyledonous species, their expression was studied in normal ... | 1994 | 7764404 |
| cotton (gossypium hirsutum l.) pollen-specific polygalacturonase mrna: tissue and temporal specificity of its promoter in transgenic tobacco. | a gene (g9) expressed during late microsporogenesis in cotton (gossypium hirsutum l.) was isolated. sequence analysis of the cdna (1.3 kb) as well as the gene (2.6 kb) revealed an open reading frame of 1233 bases encoding a protein of 43.9 kda. the coding region of the gene is interrupted by three introns. northern analysis of the rna from developing anthers showed that the transcripts appear 12 days before anthesis and that the maximal concentration of rna occurs in pollen on the day of anthesi ... | 1994 | 7858233 |
| phenylalanine ammonia-lyase from cotton (gossypium hirsutum) hypocotyls: properties of the enzyme induced by a verticillium dahliae phytotoxin. | phenylalanine ammonia-lyase (ec 4.3.1.5), induced by a verticillium dahliae phytotoxin, has been purified to electrophoretic homogeneity from cotton hypocotyls by differential ammonium sulfate fractionation and hydrophobic interaction chromatography, with a yield of 52%. the enzyme is a tetramer with a molecular weight of 332,000 to 337,000. the isoelectric point is 4.6, and no isoforms were observed. the subunits of the enzyme are unstable and breaks down to fragments with m(r)'s of 69,000 and ... | 1994 | 8043606 |
| a detailed rflp map of cotton, gossypium hirsutum x gossypium barbadense: chromosome organization and evolution in a disomic polyploid genome. | we employ a detailed restriction fragment length polymorphism (rflp) map to investigate chromosome organization and evolution in cotton, a disomic polyploid. about 46.2% of nuclear dna probes detect rflps distinguishing gossypium hirsutum and gossypium barbadense; and 705 rflp loci are assembled into 41 linkage groups and 4675 cm. the subgenomic origin (a vs. d) of most, and chromosomal identity of 14 (of 26), linkage groups is shown. the a and d subgenomes show similar recombinational length, s ... | 1994 | 7851778 |
| the distribution of gossypium hirsutum chromatin in g. barbadense germ plasm: molecular analysis of introgressive plant breeding. | cotton is unusual among major crop plants in that two cross-fertile species are widely cultivated for a common economic product, fiber. both historical evidence and classical genetic studies suggest that many improved forms of gossypium barbadense ("sea island", "egyptian", and "pima" cottons) may include chromatin derived from g. hirsutum. using 106 restriction fragment length polymorphism (rflp) loci well distributed across the cotton genome, we revealed the amount and genomic distribution of ... | 1995 | 24170011 |
| protease inhibitors of manduca sexta expressed in transgenic cotton. | to explore the effectiveness of insect derived protease inhibitors in protecting plants against insect feeding, anti-trypsin, anti-chymotrypsin and anti-elastase protease inhibitor (pi) genes from manduca sexta l. were expressed in transgenic cotton (gossypium hirsutum l.). from 198 independent transformants, 35 elite lines were further analyzed. under the control of the 35s promoter of camv, pi accumulated to approximately 0.1% of total protein, depending on the tissue analyzed. using cell-flow ... | 1995 | 24186707 |
| organization, inheritance and expression of acetohydroxyacid synthase genes in the cotton allotetraploid gossypium hirsutum. | the acetohydroxyacid synthase (ahas) gene family of the cotton ad allotetraploid gossypium hirsutum has been cloned and characterized. we have identified six different ahas genes from an analysis of genomic clones and southern blots of genomic dna. four of the six genes are organized as tandem pairs, in which the genes are separated by only 2-3 kb. conservation of restriction fragment length polymorphisms between g. hirsutum and a-genome and d-genome-containing diploid cottons was sufficient to ... | 1995 | 7640356 |
| differential induction of 3-hydroxy-3-methylglutaryl coa reductase in two cotton species following inoculation with verticillium. | gossypium barbadense cottons are typically more resistant to wilt pathogens than are gossypium hirsutum cultivars. both species make terpenoid phytoalexins in response to infection, implicating isoprenoid biosynthesis as a factor in resistance. conserved regions in plant 3-hydroxy-3-methylglutaryl coenzyme a reductase (hmgr), the first enzyme in the terpene biosynthesis pathway, were used to design polymerase chain reaction primers for cloning a fragment of a cotton hmgr gene. the clone was used ... | 1995 | 8664497 |
| effect of tropical rotation crops on meloidogyne incognita and other plant-parasitic nematodes. | in a field experiment conducted on sandy soil in florida during the 1993 season, rotation crops of castor (ricinus communis), velvetbean (mucuna deeringina), 'mississippi silver' cowpea (vigna unguiculata), american jointvetch (aeschynomene americana), 'dehapine 51' cotton (gossypium hirsutum), and 'sx-17' sorghum-sudangrass (sorghum bicolor x s. sudanense) were effective in maintaining low population densities (<12/100 cm(3) soil) of meloidogyne incognita race 1, whereas high population densiti ... | 1995 | 19277319 |
| influence of meloidogyne incognita on the water relations of cotton grown in microplots. | the effects of meloidogyne incognita on the growth and water relations of cotton were evaluated in a 2-year field study. microplots containing methyl bromide-fumigated fine sandy loam soil were infested with the nematode and planted to cotton (gossypium hirsutum l.). treatments included addition of nematodes alone, addition of nematodes plus the insecticide-nematicide aldicarb (1.7 kg/ha), and an untreated control. meloidogyne incognita population densities reached high levels in both treatments ... | 1995 | 19277313 |
| sucrose synthase localization during initiation of seed development and trichome differentiation in cotton ovules. | sucrose synthase in cotton (gossypium hirsutum l.) ovules was immunolocalized to clarify the relationship between this enzyme and (a) sucrose import/utilization during initiation of seed development, (b) trichome differentiation, and (c) cell-wall biosynthesis in these rapidly elongating "fibers." analyses focused on the period immediately before and after trichome initiation (at pollination). internal tissues most heavily immunolabeled were the developing nucellus, adjacent integument (inner su ... | 1995 | 12228669 |
| diversity in cyclic sesquiterpene production by gossypium hirsutum. | major sesquiterpene components of oil of texas race stock 810 of gossypium hirsutum were alpha- and beta-selinene. this is the seventh cyclic terpene type found to date in this genus. both alpha- and beta-selinene, along with aromadendrene, were found but only as minor components of extracts of several domestic cultivars of g. hirsutum. | 1995 | 8590634 |
| genetic fingerprinting of australian cotton cultivars with rapd markers. | rapd (random amplified polymorphic dna) markers generated by 30 random decamer primers were used to fingerprint 12 released cultivars and a breeding line of gossypium hirsutum and 1 cultivar of g. barbadense presently under cultivation in australia. among a total of 453 developed markers, 69 (15.2%) were only present (unique) in the g. barbadense cultivar pima s-7. of the remaining markers, 128 (33.3%) were fixed in all 13 g. hirsutum cultivars. in pairwise comparisons of the degree of band shar ... | 1995 | 18470223 |
| a membrane-associated form of sucrose synthase and its potential role in synthesis of cellulose and callose in plants. | sucrose synthase (susy; ec 2.4.1.13; sucrose + udp reversible udpglucose + fructose) has always been studied as a cytoplasmic enzyme in plant cells where it serves to degrade sucrose and provide carbon for respiration and synthesis of cell wall polysaccharides and starch. we report here that at least half of the total susy of developing cotton fibers (gossypium hirsutum) is tightly associated with the plasma membrane. therefore, this form of susy might serve to channel carbon directly from sucro ... | 1995 | 7568131 |
| n-acylphosphatidylethanolamine in dry and imbibing cottonseeds. amounts, molecular species, and enzymatic synthesis. | n-acylphosphatidylethanolamine (nape), an unusual acylated derivative of phosphatidylethanolamine (pe), was recently shown to be synthesized from pe and free fatty acids in cotyledons of cotton (gossypium hirsutum l.) seedlings (k.d. chapman, t.s. moore [1993] plant physiol 102: 761-769). here we report that nape is present in dry seeds of cotton and increases with time of imbibition from 2.31 nmol/seed in dry seeds to 4.26 nmol/seed in 4-h-soaked seeds. total phospholipid/seed also increased su ... | 1995 | 7480326 |
| solubilization and partial characterization of extensin fragments from cell walls of cotton suspension cultures. evidence for a covalent cross-link between extensin and pectin. | extensin, a major hydroxyproline (hyp)-rich glycoprotein in walls of cultured cells of dicotyledonous plants, is very difficult to solubilize. to learn about the nature of the insolubilization, we have tested the ability of a variety of selective hydrolytic methods, and combinations of them, to liberate extensin or fragments of extensin from suspension-culture cell walls. after the complete deglycosylation of cotton (gossypium hirsutum l.) walls, trypsinization solubilized 80% of the hyp. the se ... | 1995 | 7659756 |
| expression of two related vacuolar h(+)-atpase 16-kilodalton proteolipid genes is differentially regulated in a tissue-specific manner. | the 16-kd proteolipid subunit is the principal integral membrane protein of the vacuolar h(+)-atpase (v-atpase) complex that forms the proton channel responsible for translocating protons across lipid bilayers. two degenerate synthetic oligonucleotides, cot11 and cot12, corresponding to highly conserved transmembrane domains in all 16-kd subunits sequenced so far, were used to amplify a partial cdna of the v-atpase proteolipid subunit from cotton (gossypium hirsutum l.) by polymerase chain react ... | 1995 | 7659746 |
| characterization of mrna for a proline-rich protein of cotton fiber. | cotton (gossypium hirsutum l.) mrna (h6) is expressed predominantly in fiber cells and is present during early primary cell wall formation. however, h6 protein is found to accumulate during later stages, when active secondary cell wall formation occurs, indicating possible regulation at the translational level and function in the secondary cell wall assembly. the nucleotide-derived amino acid sequence of pck-h6 is proline rich (35 mol %) with a calculated molecular mass of 21 kd. cotton protein ... | 1995 | 7610164 |
| a cdna encoding ribosomal protein s4e from cotton (gossypium hirsutum l.). | 1995 | 7784518 | |
| characterization of a cotton (gossypium hirsutum l.) fiber mrna (fb-b6). | 1995 | 7770543 | |
| [beta]-glucan synthesis in the cotton fiber (iv. in vitro assembly of the cellulose i allomorph). | in vitro assembly of cellulose from plasma membrane extracts of the cotton (gossypium hirsutum) fiber was enriched by a combination of 3-(n-morpholino)propanesulfonic acid extraction buffer and two independent digitonin solubilization steps consisting of 0.05% digitonin (se1) followed by 1% digitonin (se2). glucan synthase activity assays revealed that, although the se2 fraction possessed higher activity, only 8.6% of the in vitro product survived acetic/nitric acid treatment. on the other hand, ... | 1995 | 12228346 |
| antioxidant response to nacl stress in a control and an nacl-tolerant cotton cell line grown in the presence of paraquat, buthionine sulfoximine, and exogenous glutathione. | a cotton (gossypium hirsutum l.) control and nacl-tolerant cell line (cv coker 312) were grown on media with or without nacl in the presence or absence of paraquat, buthionine sulfoximine, and oxidized glutathione. on medium with 150 mm nacl the nacl-tolerant cell line exhibited no reduction in growth, whereas a 96% reduction was observed in the control line. the nacl-tolerant cell line that was grown on 150 mm nacl exhibited significantly greater catalase (341%), peroxidase (319%), glutathione ... | 1996 | 12226422 |
| ribosomal protein rl44 is encoded by two subfamilies in upland cotton (gossypium hirsutum l.). | we have isolated 4 cdna clones encoding the full-length sequence of the eukaryotic ribosomal protein rl44 from upland cotton (gossypium hirsutum l.). sequencing of these clones resulted in the classification of 2 subfamilies of rl44; these subfamilies had coding regions (315 bp) which were 92% identical. rl44-1 (454 bp) and rl44-2 (485 bp) constitute subfamily 1, whereas rl44-3 (913 bp) and rl44-5 (541 bp) constitute subfamily 2. the differences in nucleotide sequences, however, occurred only at ... | 1996 | 8806588 |
| regeneration of plants from cryopreserved embryogenic cell suspension and callus cultures of cotton (gossypium hirsutum l.). | successful regeneration of cotton (gossypium hirsutum l.) plants from cryopreserved embryogenic callus and cell suspension cultures is described. the cryoprotectant mixture consisting of a modified murashige and skoog (1962) medium with sucrose (5% w/v), dmso (5% v/v) and glycerol (5% v/v) gave the highest survival rate (70%) from cell suspension cultures cryopreserved in liquid nitrogen after slow cooling (0.5 to 1.0°c/min). a cooling rate of 0.5°c/min provided a satisfactory recovery rate (30% ... | 1996 | 24178225 |
| ish-facilitated analysis of meiotic bivalent pairing. | chiasmata constitute one of the cornerstones of sexual reproduction in most eukaryotes. they mediate the reciprocal genetic exchange between homologues and are essential to the proper orientation of the homologous centromeres in meiosis i. as markers of recombination, they offer a cytological means of mapping. rather than trying to accurately count individual chiasmata, we have examined properties of the mathematical relationship between frequencies of nonadorned disomic configurations in meiosi ... | 1996 | 18469936 |
| the alcohol dehydrogenase genes of cotton. | we report here the initial characterization of the alcohol dehydrogenase (adh) gene family of the allotetraploid gossypium hirsutum, a crop plant that is highly sensitive to waterlogging. twelve adh cdnas were isolated from a library constructed from rna prepared from anaerobically stressed root tips. nine of the twelve cdnas fell into one class, while each of the other three cdnas fell into a separate class. the 3'-untranslated regions had little or no homology between classes implying that eac ... | 1996 | 8806419 |
| distribution of 5s and 18s-28s rdna loci in a tetraploid cotton (gossypium hirsutum l.) and its putative diploid ancestors. | the most widely cultivated species of cotton, gossypium hirsutum, is a disomic tetraploid (2n=4x=52). it has been proposed previously that extant a- and d-genome species are most closely related to the diploid progenitors of the tetraploid. we used fluorescent in situ hybridization (fish) to determine the distribution of 5s and 18s-28s rdna loci in the a-genome species g. herbaceum and g. arboreum, the d-genome species g. raimondii and g. thurberi, and the ad tetraploid g. hirsutum. high signal- ... | 1996 | 8662259 |
| specific binding of a verticillium dahliae phytotoxin to protoplasts of cotton, gossypium hirsutum. | the occurrence of specific, high-affinity binding sites for a protein-lipopolysaccharide (plp) phytotoxin purified from culture filtrates of a virulent vertidllium dahliae isolate has been demonstrated in cotton protoplasts. binding of the (125)i-radiolabelled plp-complex to protoplasts from cotyledon tissue was saturable and with an affinity (kd = 17.3 nm) comparable with the concentration required for biological activity. a single class of binding site, accessible at the surface of the intact ... | 1996 | 24178171 |
| establishment of molecular markers and linkage groups in two f2 populations of upland cotton. | two f2 populations of cotton (gossypium hirsutum l.) from the crosses of hs46 x marcabucag8us-1-88 (mar) and hs46 x pee dee 5363 (pd5363) were characterized for restriction fragment length polymorphisms (rflps) using dna probes. seventy-three probe/enzyme combinations were used in the hs46 x mar population analysis, which resulted in 42 informative polymorphic fragments. these 42 moleclar markers represented 26 polymorphic loci, which consisted of 15 codominant and 11 dominant (+/-) genotypes. c ... | 1996 | 24166617 |
| volatile semiochemicals released from undamaged cotton leaves (a systemic response of living plants to caterpillar damage). | cotton plants (gossypium hirsutum l.), attacked by herbivorous insects release volatile semiochemicals (chemical signals) that attract natural enemies of the herbivores to the damaged plants. we found chemical evidence that volatiles are released not only at the damaged site but from the entire cotton plant. the release of volatiles was detected from upper, undamaged leaves after 2 to 3 d of continuous larval damage on lower leaves of the same plant. compounds released systemically were (z)-3-he ... | 1996 | 12226304 |
| characterization and expression of metallothionein-like genes in cotton. | we have characterized cotton (gossypium hirsutum l.) genes encoding type 1 metallothionein-like proteins that are highly expressed in roots. little or no expression of these genes was detected in other organs and tissues. the deduced amino acid sequences have a high degree of similarity with type 1 metallothionein-like proteins from other plants, including a central hydrophobic domain flanked by conserved cysteine-rich motifs. the type 1 metallothionein-like genes of cotton are encoded by a smal ... | 1996 | 8790303 |
| systemic induction of feeding deterrents in cotton plants by feeding ofspodoptera spp. larvae. | cotton,gossypium hirsutum l., has been shown to exhibit systemic induced resistance to arthropods under certain conditions. we conducted experiments to determine the effects of previous feeding ofspodoptera exigua hübner andspodoptera littoralis (boisd.) larvae on feeding behavior, growth, and survival of larvae subsequently feeding on cotton. in one feeding choice test,s. exigua larvae preferred young leaves from undamaged control plants to undamaged young leaves from a previously damaged plant ... | 1996 | 24227615 |
| changing the dimensions of suberin lamellae of green cotton fibers with a specific inhibitor of the endoplasmic reticulum-associated fatty acid elongases. | the fibers of the green lint mutant of cotton (gossypium hirsutum l.) contain large amounts of wax and are suberized. more than 96% of the bifunctional aliphatic suberin monomers ([alpha],[omega]-alkanedioic acids and [omega]-hydroxyalkanoic acids) have chain lengths of c22 and c24 in green cotton fiber suberin. in fibers grown in the presence of s-ethyl-n,n-dipropylthiocarbamate (eptc), a specific inhibitor of the endoplasmic reticulum-associated fatty acid elongases, the aliphatic suberin mono ... | 1996 | 12226192 |
| structural characterization of genes corresponding to cotton fiber mrna, e6: reduced e6 protein in transgenic plants by antisense gene. | two genes, each corresponding to fiber mrna e6, were isolated from cotton cultivars coker 312 (gossypium hirsutum l.) and sea island (g. barbadense l.). e6 is one of the predominant fiber-specific mrnas present during early fiber development. the distinguishing feature of nucleotide-derived e6 protein is the presence of a motif where a dimer, ser-gly, is repeated several times. two of the sea island genes contained a pentameric motif, ser-gly, while one of the coker genes had one and the other h ... | 1996 | 8616253 |
| purification of (+)-delta-cadinene synthase, a sesquiterpene cyclase from bacteria-inoculated cotton foliar tissue. | a sesquiterpene cyclase whose activity is induced in a glandless, bacterial blight-resistant line of cotton (gossypium hirsutum l.) catalyses the conversion of (e,e)-farnesyl diphosphate to (+)-delta-cadinene. this enzyme was purified by a combination of salt-induced phase separation, hydroxylapatite fractionation, hydrophobic interaction and strong anion-exchange chromatography, and denaturing polyacrylamide gel electrophoresis, followed by renaturation with tween 80. the purified enzyme has a ... | 1996 | 8728715 |
| higher plants contain homologs of the bacterial cela genes encoding the catalytic subunit of cellulose synthase. | in spite of much effort, no one has succeeded in isolating and characterizing the enzyme(s) responsible for synthesis of cellulose, the major cell wall polymer of plants. we have characterized two cotton (gossypium hirsutum) cdna clones and identified one rice (oryza sativa) cdna that are homologs of the bacterial cela genes that encode the catalytic subunit of cellulose synthase. three regions in the deduced amino acid sequences of the plant cela gene products are conserved with respect to the ... | 1996 | 8901635 |
| in vitro prenylation of the small gtpase rac13 of cotton. | previous work (d.p. delmer, j. pear, a. andrawis, d. stalker [1995] mol gen genet 248: 43-51) has identified a gene in cotton (gossypium hirsutum), rac13, that encodes a small, signal-transducing gtpase and shows high expression in the fiber at the time of transition from primary to secondary wall synthesis. since rac13 may be important in signal transduction pathway(s), regulating the onset of fiber secondary wall synthesis, we continue to characterize rac13 by determining its ability to underg ... | 1996 | 12226460 |
| meloidogyne incognita infested soil amended with chicken litter. | the effects of chicken litter on meloidogyne incognita in cotton, gossypium hirsutum cv. dpl50 were determined in field microplots. litters (manure and pine-shaving bedding) from a research facility and a commercial broiler house were used. treatments consisted of 0.25%, 0.5%, and 1% litter by dry weight of soil for each kind of litter. three control treatments consisted of soil not amended with litter, with and without nematodes, and one treatment to which mineral fertilizer was added at a nitr ... | 1996 | 19277155 |
| impact of soil texture on the reproductive and damage potentials of rotylenchulus reniformis and meloidogyne incognita on cotton. | the effects of soil type and initial inoculum density (pi) on the reproductive and damage potentials of meloidogyne incognita and rotylenchulus reniformis on cotton were evaluated in microplot experiments from 1991 to 1993. the equilibrium nematode population density for r. reniformis on cotton was much greater than that of m. incognita, indicating that cotton is a better host for r. reniformis than m. incognita. reproduction of m. incognita was greater in coarse-textured soils than in fine-text ... | 1996 | 19277171 |
| frequency and geographical distribution of plant-parasitic nematodes on cotton in georgia. | a survey was conducted to examine the geographical distribution of plant-parasitic nematodes in georgia cotton fields. a total of 778 fields in 11 georgia counties were sampled from 1 september through 15 december 1995. four nematode genera parasitic on cotton were found in this survey: meloidogyne spp., rotylenchulus sp., hoplolaimus sp., and belonolaimus sp. meloidogyne spp. was present in 9% to 56% of the fields in individual counties. rotylenchulus sp. was found in 10 counties, hoplolaimus s ... | 1996 | 19277192 |
| characterization and expression of chitinase and 1,3-beta-glucanase genes in cotton. | we have isolated cdna clones representing mrnas encoding chitinase and 1,3-beta-glucanase in cotton (gossypium hirsutum l.) leaves. the chitinase clones were sequenced and found to encode a 28,806 da protein with 71% amino acid sequence similarity to the sk2 chitinase from potato (solanum tuberosum). the 1,3-beta-glucanase clones encoded a 37,645 da protein with 57.6% identity to a 1,3-beta-glucanase from soybean (glycine max). northern blot analyses showed that chitinase mrna is induced in plan ... | 1996 | 8806421 |
| de novo biosynthesis of volatiles induced by insect herbivory in cotton plants. | in response to insect feeding on the leaves, cotton (gossypium hirsutum l.) plants release elevated levels of volatiles, which can serve as a chemical signal that attracts natural enemies of the herbivore to the damaged plant. pulse-labeling experiments with [13c]co2 demonstrated that many of the volatiles released, including the acyclic terpenes (e,e)-[alpha]-farnesene, (e)-[beta]-farnesene, (e)-[beta]-ocimene, linalool, (e)-4,8-dimethyl-1,3,7-nonatriene, and (e/e)-4,8,12-trimethyl-1,3,7,11-tri ... | 1997 | 12223763 |
| resistance to meloidogyne incognita race 3 and rotylenchulus reniformis in wild accessions of gossypium hirsutum and g. barbadense from mexico. | forty-six accessions of g. hirsutum and two of g. barbadense were examined for resistance to meloidogyne incognita race 3 and rotylenchulus reniformis in environmental growth chamber experiments, with the objective of finding new sources of resistance. only the g. barbadense accessions, tx-1347 and tx-1348, supported significantly less reproduction by r. reniformis than the susceptible control, deltapine 16 (usda accession sa-1186). however, they were highly susceptible to m. incognita race 3. t ... | 1997 | 19274280 |
| tolerance to rotylenchulus reniformis and resistance to meloidogyne incognita race 3 in high-yielding breeding lines of upland cotton. | field experiments in 1992 and 1994 were conducted to determine the effect of rotylenchulus reniformis, reniform nematode, on lint yield and fiber quality of 10 experimental breeding lines of cotton (gossypium hirsutum) in untreated plots or plots fumigated with 1,3-dichloropropene. controls were la. rn 1032, a germplasm line possessing some resistance to r. reniformis, and stoneville 453, a cultivar that is susceptible to reniform nematode. several breeding lines produced greater lint yields tha ... | 1997 | 19274165 |
| inoculum densities of fusarium oxysporum f. sp. vasinfectum and meloidogyne incognita in relation to the development of fusarium wilt and the phenology of cotton plants (gossypium hirsutum). | abstract development of fusarium wilt in upland cotton (gossypium hirsutum) usually requires infections of plants by both meloidogyne incognita and fusarium oxysporum f. sp. vasinfectum. in this study, the soil densities of m. incognita and f. oxysporum f. sp. vasinfectum and the incidence of fusarium wilt in three field sites were determined in 1982-1984. multiple regression analysis of percent incidence of fusarium wilt symptoms on population densities of m. incognita and f. oxysporum f. sp. v ... | 1997 | 18945178 |
| quantum yields and rate constants of photochemical and nonphotochemical excitation quenching (experiment and model). | sunflower (helianthus annuus l.), cotton (gossypium hirsutum l.), tobacco (nicotiana tabacum l.), sorghum (sorghum bicolor moench.), amaranth (amaranthus cruentus l.), and cytochrome b6f complex-deficient transgenic tobacco leaves were used to test the response of plants exposed to differnt light intensities and co2 concentrations before and after photoinhibition at 4000 [mu]mol photons m-2 s-1 and to thermoinhibition up to 45[deg]c. quantum yields of photochemical and nonphotochemical excitatio ... | 1997 | 12223845 |
| use of meiotic fish for identification of a new monosome in gossypium hirsutum l. | the extensive use of molecular cytogenetics in human genetics and clinical diagnostics indicates that analogous applications in plants are highly feasible. one sort of application would be the identification of new aneuploids, which traditionally involves either direct karyotypic identification, which is feasible in only a few plant species, or tests with markers (cytogenetic, genetic, or molecular), which require sexual hybridization and at least one subsequent seed or plant generation. we have ... | 1997 | 9061912 |
| evaluation of nemx, a new cultivar of cotton with high resistance to meloidogyne incognita. | the level of resistance to root-knot nematode, meloidogyne incognita, in nemx, a new cultivar of the acala-type upland cotton, was evaluated in relation to four resistant breeding lines (n6072, n8577, n901, and n903) and four susceptible cultivars (maxxa, sj2, royale, and prema). in growth pouch tests, an average of only 4 nematode egg masses was produced on roots of nemx or the resistant lines, compared to a significantly higher average of 21 on the susceptible cultivars. in pot tests, the nema ... | 1997 | 19274190 |
| sequence and rt-pcr expression analysis of two peroxidases from arabidopsis thaliana belonging to a novel evolutionary branch of plant peroxidases. | cdna clones encoding two new arabidopsis thaliana peroxidases, atp 1a and atp 2a, have been identified by searching the arabidopsis database of expressed sequence tags (dbest). they represent a novel branch of hitherto uncharacterized plant peroxidases which is only 35% identical in amino acid sequence to the well characterized group of basic plant peroxidases represented by the horseradish (armoracia rusticana) isoperoxidases hrp c, hrp e5 and the similar arabidopsis isoperoxidases atp ca, atp ... | 1997 | 9132061 |
| the differential expression of sucrose synthase in relation to diverse patterns of carbon partitioning in developing cotton seed. | developing cotton (gossypium hirsutum l.) seed exhibits complex patterns of carbon allocation in which incoming sucrose (suc) is partitioned to three major sinks: the fibers, seed coat, and cotyledons, which synthesize cellulose, starch, and storage proteins or oils, respectively. in this study we investigated the role of suc synthase (susy) in the mobilization of suc into such sinks. assessments of susy gene expression at various levels led to the surprising conclusion that, in contrast to that ... | 1997 | 12223814 |
| cotton fibers can undergo cell division. | ovular culture was used to determine the cell cycle aspects of cotton fiber cells. each ovule (gossypium hirsutum, cultivar, md51 ne) grown under the conditions used has ~10 000 fiber cells at 4 d postanthesis. about 25% of these cells divide when ovules are cultured at 34c. mitosis occurs after fiber cells differentiate, producing multicelled fibers. the basal and tip cells of multicelled fibers have the same characteristics as the polar ends of single-celled fibers. most cell division occurs i ... | 1997 | 21708678 |
| identification of a delta-tip cdna clone and determination of related a and d genome subfamilies in gossypium species. | tonoplast intrinsic proteins (tips) have been implicated in the process of cell elongation, such as occurs in the developing cotton fiber. we have isolated a cdna clone (997 bp in length) from a cotton (gossypium hirsutum l.) library which putatively encodes a protein of 248 residues (mr 25079) with 85% identity to arabidopsis delta-tip. the derived amino acid sequence included two conserved sequences associated with major intrinsic proteins (sgxhxnpa at residues 78 to 85, npa residues at 197 to ... | 1997 | 9177317 |
| identification of a cotton fiber-specific acyl carrier protein cdna by differential display. | transcripts from immature fibers and stripped ovules (fibers removed) of cotton (gossypium hirsutum l.) were compared by differential display to identify cdna fragments that represent mrnas that are expressed primarily in cotton fibers. eight independent fiber-specific cdna fragments were isolated. one of these cdnas had strong sequence similarity with acyl carrier protein (acp). a full-length cdna for the cotton fiber-specific acp was isolated using a pcr cdna library screening technique. this ... | 1997 | 9130594 |
| interference among cotton neighbours after differential reproductive damage. | in indeterminate plant species, the rate of vegetative growth usually declines during the stage of active reproductive growth. fruit shedding, as induced by insect herbivores, could counteract this decline. due to the relative increase in vegetative growth, plants that have suffered reproductive damage could be better able to intercept light and acquire soil resources than undamaged plants. if so, plants with damaged neighbours might grow less than their counterparts with smaller, undamaged neig ... | 1997 | 28307540 |
| genetic diversity evaluation of some elite cotton varieties by rapd analysis. | random amplified polymorphic dna (rapd) analysis was used to evaluate the genetic diversity of elite commercial cotton varieties. twenty two varieties belonging to gossypium hirsutum l. and one to g. arboreum l. were analyzed with 50 random decamer primers using the polymerase chain reaction (pcr). forty nine primers detected polymorphism in all 23 cotton varieties, while one produced monomorphic amplification profiles. a total of 349 bands were amplified, 89.1% of which were polymorphic. cluste ... | 1997 | 19352756 |
| isolation of a cotton cap gene: a homologue of adenylyl cyclase-associated protein highly expressed during fiber elongation. | the cdna encoding cap (adenylyl cyclase-associated protein) was isolated from a cotton (gossypium hirsutum) fiber cdna library. the cdna (ghcap) contained an open reading frame that encoded 471 amino acid residues. rna blot analysis showed that the cotton cap gene was expressed mainly in young fibers. | 1998 | 10050322 |
| the lance nematode, hoplolaimus magnistylus, on cotton in arkansas. | the population density of hoplolaimus magnistylus, a lance nematode, in cotton was determined at planting, mid-season, and harvest during the 1995 and 1996 growing seasons for a poinsett county, arkansas field. nematode populations increased from planting to harvest in 1995 but declined in 1996. application of aldicarb at planting at rates of 0.59 or 0.84 kg a.i./ha did not influence either nematode population density or cotton yield. this study indicates that h. magnistylus is not a serious pes ... | 1998 | 19274251 |
| structural analysis of a hmg-coa-reductase pseudogene: insights into evolutionary processes affecting the hmgr gene family in allotetraploid cotton (gossypium hirsutum l.). | structural analysis of hmg-coa reductase (hmgr) genes in the allotetraploid cotton species gossypium hirsutum l. revealed the first-known existence of a pseudogene, psihmg5, for this important enzyme. complete sequencing of the genomic clone hmg5 unveiled several deleterious lesions, resulting in an organization that departed significantly from the linear canonical hmgr gene structure. although analysis of the 5' flanking region indicated a promoter-like composition based on comparison with othe ... | 1998 | 9799357 |
| a fiberless seed mutation in cotton is associated with lack of fiber cell initiation in ovule epidermis and alterations in sucrose synthase expression and carbon partitioning in developing seeds | fiber cell initiation in the epidermal cells of cotton (gossypium hirsutum l.) ovules represents a unique example of trichome development in higher plants. little is known about the molecular and metabolic mechanisms controlling this process. here we report a comparative analysis of a fiberless seed (fls) mutant (lacking fibers) and a normal (fls) mutant to better understand the initial cytological events in fiber development and to analyze the metabolic changes that are associated with the loss ... | 1998 | 9765525 |
| genes involved in osmoregulation during turgor-driven cell expansion of developing cotton fibers are differentially regulated. | cotton (gossypium hirsutum l.) fibers are single-celled trichomes that synchronously undergo a phase of rapid cell expansion, then a phase including secondary cell wall deposition, and finally maturation. to determine if there is coordinated regulation of gene expression during fiber expansion, we analyzed the expression of components involved in turgor regulation and a cytoskeletal protein by measuring levels of mrna and protein accumulation and enzyme activity. fragments of the genes for the p ... | 1998 | 9536073 |
| moderately high temperatures inhibit ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) activase-mediated activation of rubisco | we tested the hypothesis that light activation of ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) is inhibited by moderately elevated temperature through an effect on rubisco activase. when cotton (gossypium hirsutum l.) or wheat (triticum aestivum l.) leaf tissue was exposed to increasing temperatures in the light, activation of rubisco was inhibited above 35 and 30 degreesc, respectively, and the relative inhibition was greater for wheat than for cotton. the temperature-induced inhib ... | 1998 | 9490757 |
| growth and mitotic potential of multicelled fibers of cotton (malvaceae). | we tested the hypothesis that the growth of multicelled cotton fibers of gossypium hirsutum, cultivar md51 ne, occurs exclusively within the tip cell. direct cellular measurements proved the hypothesis incorrect. the results show that all cells within a fiber grow and that the relative growth of the tip cell is reduced as the number of cells per fiber increases. also, measurements of two- and three-celled fibers show that the two daughter nuclei in two-celled fibers differ. the ability to divide ... | 1998 | 21684876 |