Publications

TitleAbstractYear
Filter
PMID(sorted descending)
Filter
genetic dissection of the fuzzless seed trait in gossypium barbadense.cotton fibres are single-celled trichomes arising from the epidermal cells of the seed coat and may be either long (lint) or very short (fuzz). the dominant fuzzless n1 of gossypium hirsutum is a defective allele of the at-subgenome homoeolog of myb25-like, but the genetic components underlying the recessive fuzzless trait from g. barbadense (gb) are unknown. we have identified five genetic loci, including a major contributing locus containing myb25-like_dt, associated with gb fuzzless seeds bas ...201829351643
association between ssr markers and fibre traits in sea island cotton (gossypium barbadense) germplasm resources.identification of molecular markers associated with fibre traits can accelerate cotton marker-assisted selection (mas) programmes. in this study, gossypium barbadense germplasm accessions with diverse origins (n = 123) were used to perform association analysis of fibre traits with 120 polymorphic simple sequence repeat (ssr) markers. in total, 120 polymorphic primer pairs amplified 258 loci with a mean of 2.15 loci per primer. population structure analysis identified three main clusters for the ...201729321342
artificial intelligence and regression analysis for cd(ii) ion biosorption from aqueous solution by gossypium barbadense waste.in this study, batch biosorption experiments were conducted to determine the removal efficiency of cd(ii) ion from aqueous solutions by gossypium barbadense waste. the biosorbent was characterized by fourier transform infrared spectroscopy (ftir) and scanning electron microscopy (sem) connected with energy dispersive x-ray (edx). the sorption mechanism was described by complexation/chelation of cd2+ with the functional groups of o-h, c=o, -coo-, and c-o, as well as, cation-exchange with mg2+ and ...201729235028
qtl analysis of transgressive nematode resistance in tetraploid cotton reveals complex interactions in chromosome 11 regions.transgressive segregation in cotton (gossypium spp.) provides an important approach to enhance resistance to the major pest root-knot nematode (rkn) meloidogyne incognita. our previous studies reported transgressive rkn resistance in an intraspecific gossypium hirsutum resistant nemx × susceptible sj-2 recombinant inbred line (ril) population and early generations of interspecific cross gossypium barbadense (susceptible pima s-7) × g. hirsutum (nemx). however, the underlying functional mechanism ...201729209344
long noncoding rnas involve in resistance to verticillium dahliae, a fungal disease in cotton.long noncoding rnas (lncrnas) have several known functions in plant development, but their possible roles in responding to plant disease remain largely unresolved. in this study, we described a comprehensive disease-responding lncrna profiles in defence against a cotton fungal disease verticillium dahliae. we further revealed the conserved and specific characters of disease-responding process between two cotton species. conservatively for two cotton species, we found the expression dominance of ...201729149461
genetic diversity, linkage disequilibrium, and association mapping analyses of gossypium barbadense l. germplasm.limited polymorphism and narrow genetic base, due to genetic bottleneck through historic domestication, highlight a need for comprehensive characterization and utilization of existing genetic diversity in cotton germplasm collections. in this study, 288 worldwide gossypium barbadense l. cotton germplasm accessions were evaluated in two diverse environments (uzbekistan and usa). these accessions were assessed for genetic diversity, population structure, linkage disequilibrium (ld), and ld-based a ...201729136656
comparative analysis of the cytology and transcriptomes of the cytoplasmic male sterility line h276a and its maintainer line h276b of cotton (gossypium barbadense l.).in this study, the tetrad stage of microspore development in a new cotton (gossypium barbadense l.) cytoplasmic male sterility (cms) line, h276a, was identified using paraffin sections at the abortion stage. to explore the molecular mechanism underlying cms in cotton, a comparative transcriptome analysis between the cms line h276a and its maintainer line h276b at the tetrad stage was conducted using an illumina hiseq 4000 platform. the comparison of h276a with h276b revealed a total of 64,675 ge ...201729068396
the island cotton nbs-lrr gene gbana1 confers resistance to the non-race 1 verticillium dahliae isolate vd991.wilt caused by verticillium dahliae significantly reduces cotton yields, as host resistance in commercially cultivated gossypium species is lacking. understanding the molecular bases of disease resistance in non-commercial gossypium species could galvanize the development of verticillium wilt resistance in the cultivated species. nucleotide binding site-leucine-rich repeat (nbs-lrr) proteins play a central role in plant defense against pathogens. in this study, we focused on the relationship bet ...201729052967
the jasmonate zim-domain gene family mediates ja signaling and stress response in cotton.jasmonate zim-domain (jaz) family proteins are involved in regulating diverse biological processes in plants. however, their functions have not been well characterized in cotton (gossypium spp.). in the present study, 13, 15, 25 and 30 jaz genes were identified in gossypium arboretum, gossypium raimondii, gossypium barbadense and gossypium hirsutum, respectively, based on gene homology. selection and variation analyses showed that the single nucleotide polymorphism (snp) density of ghjaz genes i ...201729036515
genetic and phenotypic effects of chromosome segments introgressed from gossypium barbadense into gossypium hirsutum.mbi9915 is an introgression cotton line with excellent fiber quality. it was obtained by advanced backcrossing and continuous inbreeding from an interspecific cross between the upland cotton (gossypium hirsutum) cultivar ccri36 as the recurrent parent and the sea island cotton (g. barbadense) cultivar hai1, as the donor parent. to study the genetic effects of the introgressed chromosome segments in g. hirsutum, an f2 secondary segregating population of 1537 individuals was created by crossing mb ...201728931074
comparative transcriptome analysis of cotton fiber development of upland cotton (gossypium hirsutum) and chromosome segment substitution lines from g. hirsutum × g. barbadense.how to develop new cotton varieties possessing high yield traits of upland cotton and superior fiber quality traits of sea island cotton remains a key task for cotton breeders and researchers. while multiple attempts bring in little significant progresses, the development of chromosome segment substitution lines (cssls) from gossypium barbadense in g. hirsutum background provided ideal materials for aforementioned breeding purposes in upland cotton improvement. based on the excellent fiber perfo ...201728886694
transcriptome analysis suggests that chromosome introgression fragments from sea island cotton (gossypium barbadense) increase fiber strength in upland cotton (gossypium hirsutum).as high-strength cotton fibers are critical components of high quality cotton, developing cotton cultivars with high-strength fibers as well as high yield is a top priority for cotton development. recently, chromosome segment substitution lines (cssls) have been developed from high-yield upland cotton (gossypium hirsutum) crossed with high-quality sea island cotton (g. barbadense). here, we constructed a cssl population by crossing ccri45, a high-yield upland cotton cultivar, with hai1, a sea is ...201728874383
histochemical analyses reveal that stronger intrinsic defenses in gossypium barbadense than in g. hirsutum are associated with resistance to verticillium dahliae.verticillium wilt, caused by verticillium dahliae kleb., is a serious threat to cotton (gossypium spp.) crop production. to enhance our understanding of the plant's complex defensive mechanism, we examined colonization patterns and interactions between v. dahliae and two cotton species, the resistant g. barbadense and the susceptible g. hirsutum. microscopic examinations and grafting experiments showed that the progression of infection was restricted within g. barbadense. at all pre- and post-in ...201728850286
regional association analysis-based fine mapping of three clustered qtl for verticillium wilt resistance in cotton (g. hirsutum. l).verticillium wilt is one of the most destructive diseases affecting global cotton production. the most effective way to control wilt disease has been the development of new cotton varieties that are resistant to vw. vw-resistant upland cotton cultivars have been created in both the usa and china by gossypium barbadense introgression. more than 100 vw resistance quantitative trait loci have been detected.201728841857
the variation in the rhizosphere microbiome of cotton with soil type, genotype and developmental stage.plant roots and soil microorganisms interact with each other mainly in the rhizosphere. changes in the community structure of the rhizosphere microbiome are influenced by many factors. in this study, we determined the community structure of rhizosphere bacteria in cotton, and studied the variation of rhizosphere bacterial community structure in different soil types and developmental stages using tm-1, an upland cotton cultivar (gossypium hirsutum l.) and hai 7124, a sea island cotton cultivar (g ...201728638057
the ectopic overexpression of the cotton ve1 and ve2-homolog sequences leads to resistance response to verticillium wilt in arabidopsis.verticillium wilt, caused by the verticillium dahliae phytopathogen, is a devastating disease affecting many economically important crops. a receptor-like protein (rlp) gene, ve1, has been reported to confer resistance to v. dahliae in tomato plants, but few genes have been found to be involved in cotton verticillium wilt resistance. here, we cloned two rlp gene homologs, gossypium barbadense resistance gene to verticillium dahliae 1 (gbavd1) and gbavd2, from the verticillium wilt-resistant cult ...201728611793
detoxification of fusaric acid by the soil microbe mucor rouxii.fusarium oxysporum f. sp. vasinfectum race 4 (vcg0114), which causes root rot and wilt of cotton (gossypium hirsutum and g. barbadense), has been identified recently for the first time in the western hemisphere in certain fields in the san joaquin valley of california. this pathotype produces copious quantities of the plant toxin fusaric acid (5-butyl-2-pyridinecarboxylic acid) compared to other isolates of f. oxysporum f. sp. vasinfectum (fov) that are indigenous to the united states. fusaric a ...201728538103
quantitative trait locus mapping for verticillium wilt resistance in an upland cotton recombinant inbred line using snp-based high density genetic map.verticillium wilt (vw) caused by verticillium dahlia kleb is one of the most destructive diseases of cotton. numerous efforts have been made to improve the resistance of upland cotton against vw, with little progress achieved due to the paucity of upland cotton breeding germplasms with high level of resistance to vw. gossypium barbadense was regarded as more resistant compared to upland cotton; however, it is difficult to apply the resistance from g. barbadense to upland cotton improvement becau ...201728424708
genome-wide comparative analysis of nbs-encoding genes in four gossypium species.nucleotide binding site (nbs) genes encode a large family of disease resistance (r) proteins in plants. the availability of genomic data of the two diploid cotton species, gossypium arboreum and gossypium raimondii, and the two allotetraploid cotton species, gossypium hirsutum (tm-1) and gossypium barbadense allow for a more comprehensive and systematic comparative study of nbs-encoding genes to elucidate the mechanisms of cotton disease resistance.201728403834
cotton defense induction patterns under spatially, temporally and quantitatively varying herbivory levels.in its defense against herbivores, cotton (gossypium sp.) relies in part on the production of a set of inducible, non-volatile terpenoids. under uniform damage levels, in planta allocation of induced cotton terpenoids has been found to be highest in youngest leaves, supporting assumptions of the optimal defense theory (odt) which predicts that plants allocate defense compounds to tissues depending on their value and the likelihood of herbivore attack. however, our knowledge is limited on how var ...201728270830
a novel gossypium barbadense erf transcription factor, gberfb, regulation host response and resistance to verticillium dahliae in tobacco.ethylene-responsive factors (erfs) are commonly considered to play an important role in pathogen defense responses. however, only few of erf members have been characterized in sea island cotton (gossypium barbadense). here, we reported a novel ap2/erf transcription factors gene, named gberfb which was cloned and identified from sea island cotton by race. the expression of gberfb was significantly induced by treatments with ethylene, methyl jasmonate, salicylic acid, wounding, h2o2 and verticilli ...201728250589
genomic insights into divergence and dual domestication of cultivated allotetraploid cottons.cotton has been cultivated and used to make fabrics for at least 7000 years. two allotetraploid species of great commercial importance, gossypium hirsutum and gossypium barbadense, were domesticated after polyploidization and are cultivated worldwide. although the overall genetic diversity between these two cultivated species has been studied with limited accessions, their population structure and genetic variations remain largely unknown.201728219438
genetic diversity among exotic cotton accessions as for qualitative and quantitative traits.studying genetic diversity among a group of genotypes is important in genetic breeding because identifying hybrid combinations of greater heterotic effect also increases the chance of obtaining plants with favorable allele combinations in an intra-population selection program. the objective of this study was to compare different types of long and extra-long staple cotton and their genetic diversity in relation to the fiber traits and some agronomic traits in order to grant breeding programs. div ...201728198514
genome-wide identification of the tify gene family in three cultivated gossypium species and the expression of jaz genes.tify proteins are plant-specific proteins containing tify, jaz, ppd and zml subfamilies. a total of 50, 54 and 28 members of the tify gene family in three cultivated cotton species-gossypium hirsutum, gossypium barbadense and gossypium arboretum-were identified, respectively. the results of phylogenetic analysis showed that these tify genes were divided into eight clusters. the different clusters of gene family members often have similar gene structures, including the number of exons. the result ...201728186193
genome-wide divergence, haplotype distribution and population demographic histories for gossypium hirsutum and gossypium barbadense as revealed by genome-anchored snps.use of 10,129 singleton snps of known genomic location in tetraploid cotton provided unique opportunities to characterize genome-wide diversity among 440 gossypium hirsutum and 219 g. barbadense cultivars and landrace accessions of widespread origin. using the snps distributed genome-wide, we examined genetic diversity, haplotype distribution and linkage disequilibrium patterns in the g. hirsutum and g. barbadense genomes to clarify population demographic history. diversity and identity-by-state ...201728128280
island cotton enhanced disease susceptibility 1 gene encoding a lipase-like protein plays a crucial role in response to verticillium dahliae by regulating the sa level and h2o2 accumulation.cotton is one of the most economically important crops, but most cultivated varieties lack adequate innate immunity or resistance to verticillium wilt. this results in serious losses to both yield and fiber quality. to identify the genetic resources for innate immunity and understand the pathways for pathogen defenses in this crop, here we focus on orthologs of the central arabidopsis thaliana defense regulator enhanced disease susceptibility 1 (eds1). the full-length cdna of gbeds1 was obtained ...201628018374
naturally occurring high oleic acid cottonseed oil: identification and functional analysis of a mutant allele of gossypium barbadense fatty acid desaturase-2.some naturally occurring cotton accessions contain commercially attractive seed oil fatty acid profiles. the likely causal factor for a high-oleate trait in pima cotton ( gossypium barbadense ) accession gb-713 is described here. vegetable oils are broadly used in the manufacture of many human and animal nutritional products, and in various industrial applications. along with other well-known edible plant oils from soybean, corn, and canola, cottonseed oil is a valuable commodity. cottonseed oil ...201727988886
expression in pichia pastoris and characterization of two novel dirigent proteins for atropselective formation of gossypol.we established an efficient fed-batch fermentation process for two novel dirigent proteins from cotton plants, gbdir2 from gossypium barbadense and ghdir3 from g. hirsutum, using the engineered pichia pastoris glycoswitch® superman5 strain to prevent hyperglycosylation. the two (his)6-tagged proteins were purified by metal-chelate affinity chromatography and obtained in quantities of 12 and 15 mg l(-1) of culture volume, respectively. glycosylation sites were identified for the native and for th ...201727858135
quantitative trait loci analysis of verticillium wilt resistance in interspecific backcross populations of gossypium hirsutum × gossypium barbadense.verticillium wilt (vw) caused by verticillium dahliae (kleb) is one of the most destructive diseases of cotton. the identification of highly resistant qtls or genes in the whole cotton genome is quite important for developing a vw-resistant variety and for further molecular design breeding.201627814678
characterization, expression, and functional analysis of a novel nac gene associated with resistance to verticillium wilt and abiotic stress in cotton.elucidating the mechanism of resistance to biotic and abiotic stress is of great importance in cotton. in this study, a gene containing the nac domain, designated gbnac1, was identified from gossypium barbadense l. homologous sequence alignment indicated that gbnac1 belongs to the tern subgroup. gbnac1 protein localized to the cell nucleus. gbnac1 was expressed in roots, stems, and leaves, and was especially highly expressed in vascular bundles. functional analysis showed that cotton resistance ...201627784753
spatio-temporal, genotypic, and environmental effects on plant soluble protein and digestible carbohydrate content: implications for insect herbivores with cotton as an exemplar.plant soluble protein and digestible carbohydrate content significantly affect insect herbivore fitness, but studies reporting plant protein and carbohydrate content are rare. instead, the elements nitrogen and carbon often are used as surrogates for plant protein and digestible carbohydrate content, respectively. however, this is problematic for two reasons. first, carbon is found in all organic molecules, which precludes strong correlations with ecologically important dietary macronutrients (e ...201627738861
snp marker discovery in pima cotton (gossypium barbadense l.) leaf transcriptomes.the objective of this study was to explore the known narrow genetic diversity and discover single-nucleotide polymorphic (snp) markers for marker-assisted breeding within pima cotton (gossypium barbadense l.) leaf transcriptomes. cdna from 25-day plants of three diverse cotton genotypes [pima s6 (ps6), pima s7 (ps7), and pima 3-79 (p3-79)] was sequenced on illumina sequencing platform. a total of 28.9 million reads (average read length of 138 bp) were generated by sequencing cdna libraries of th ...201627721653
early pre-hispanic use of indigo blue in peru.archaeological research has identified the use of cultivated cotton (gossypium barbadense) in the ancient andes dating back to at least 7800 years ago. because of unusual circumstances of preservation, 6000-year-old cotton fabrics from the preceramic site of huaca prieta on the north coast of peru retained traces of a blue pigment that was analyzed and positively identified as an indigoid dye (indigotin), making it the earliest known use of indigo in the world, derived most likely from indigofer ...201627652337
identification of chromosome segment substitution lines of gossypium barbadense introgressed in g. hirsutum and quantitative trait locus mapping for fiber quality and yield traits.chromosome segment substitution lines mbi9804, mbi9855, mbi9752, and mbi9134, which were obtained by advanced backcrossing and continuously inbreeding from an interspecific cross between ccri36, a cultivar of upland cotton (gossypium hirsutum) as the recurrent parent, and hai1, a cultivar of sea island cotton (g. barbadense) as the donor parent, were used to construct a multiple parent population of (mbi9804×mbi9855)×(mbi9752×mbi9134). the segregating generations of double-crossed f1 and f2 and ...201627603312
genetic effects and heterosis of yield and yield component traits based on gossypium barbadense chromosome segment substitution lines in two gossypium hirsutum backgrounds.we hybridized 10 chromosome segment substitution lines (cssls) each from two cssl populations and produced 50 f1 hybrids according to north carolina design ii. we analyzed the genetic effects and heterosis of yield and yield components in the f1 hybrids and parents in four environments via the additive-dominance genetic model. yield and yield components of the cssls were controlled by combined additive and dominance effects, and lint percentage was mainly controlled by additive effects, but boll ...201627348815
molecular cloning and analysis of a receptor-like promoter of gbvdr3 gene in sea island cotton.verticillium wilt caused by soil borne fungus verticillium dahliae could significantly reduce cotton yield. the ve1 homologous gene gbvdr3 is resistant to verticillium wilt. in order to understand of the function of the promoter gbvdr3 in gossypium barbadense, the promoter region of the receptor-like gene gbvdr3 was obtained by genome walking, and the cis-element in the promoter was identified using the place software in this study. the sequence analysis showed that the promoter contained elemen ...201627323087
independent domestication of two old world cotton species.domesticated cotton species provide raw material for the majority of the world's textile industry. two independent domestication events have been identified in allopolyploid cotton, one in upland cotton (gossypium hirsutum l.) and the other to egyptian cotton (gossypium barbadense l.). however, two diploid cotton species, gossypium arboreum l. and gossypium herbaceum l., have been cultivated for several millennia, but their status as independent domesticates has long been in question. using geno ...201627289095
a comparative transcriptome analysis of two sets of backcross inbred lines differing in lint-yield derived from a gossypium hirsutum × gossypium barbadense population.upland cotton (gossypium hirsutum l.) is the most important fiber crop, and its lint-yield improvement is impeded due to its narrow genetic base and the lack of understanding of the genetic basis of yield. backcross inbred lines (bils) or near-isogenic lines (nils) in the same genetic background differing in lint yield, developed through advanced backcrossing, provide an important genomic resource to study the molecular genetic basis of lint yield. in the present study, a high-yield (hy) group a ...201627256327
screening and chromosome localization of two cotton bac clones.two bacterial artificial chromosome (bac) clones (350b21 and 299n22) of pima 90-53 cotton [gossypium barbadense linnaeus, 1753 (2n=4x=52)] were screened from a bac library using ssr markers. strong hybridization signals were detected at terminal regions of all a genome (sub-genome) chromosomes, but were almost absent in d genome (sub-genome) chromosomes with bac clone 350b21 as the probe. the results indicate that specific sequences, which only exist at the terminal parts of a genome (sub-genome ...201627186333
identification of multiple stress responsive genes by sequencing a normalized cdna library from sea-land cotton (gossypium barbadense l.).plants often face multiple stresses including drought, extreme temperature, salinity, nutrition deficiency and biotic stresses during growth and development. all the stresses result in a series of physiological and metabolic reactions and then generate reversible inhibition of metabolism and growth and can cause seriously irreversible damage, even death. at each stage of cotton growth, environmental stress conditions pose devastating threats to plant growth and development, especially yield and ...201627031331
to be a flower or fruiting branch: insights revealed by mrna and small rna transcriptomes from different cotton developmental stages.the architecture of the cotton plant, including fruit branch formation and flowering pattern, is the most important characteristic that directly influences light exploitation, yield and cost of planting. nulliplex branch is a useful phenotype to study cotton architecture. we used rna sequencing to obtain mrna and mirna profiles from nulliplex- and normal-branch cotton at three developmental stages. the differentially expressed genes (degs) and mirnas were identified that preferentially/specifica ...201626983497
an ethylene response-related factor, gberf1-like, from gossypium barbadense improves resistance to verticillium dahliae via activating lignin synthesis.an ethylene response-related factor, gberf1-like, from gossypium barbadense cv. '7124' involved in the defence response to verticillium dahliae was characterized. gberf1-like transcripts present ubiquitously in various tissues, with higher accumulation in flower organs. gberf1-like was also responsive to defence-related phytohormones and v. dahliae infection. the downregulation of gberf1-like increased the susceptibility of cotton plants to v. dahliae infection, while overexpression of this gene ...201626971283
postinfection development of rotylenchulus reniformis on resistant gossypium barbadense accessions.the reniform nematode (rotylenchulus reniformis) causes significant cotton (gossypium hirsutum) losses in the southeastern united states. the research objective was to describe the effects of two resistant g. barbadense lines (cultivar tx 110 and accession gb 713) on development and fecundity of reniform nematode. nematode development and fecundity were evaluated on the resistant lines and susceptible g. hirsutum cultivar deltapine 16 in three repeated growth chamber experiments. nematode develo ...201526941458
the thioredoxin gbnrx1 plays a crucial role in homeostasis of apoplastic reactive oxygen species in response to verticillium dahliae infection in cotton.examining the proteins that plants secrete into the apoplast in response to pathogen attack provides crucial information for understanding the molecular mechanisms underlying plant innate immunity. in this study, we analyzed the changes in the root apoplast secretome of the verticillium wilt-resistant island cotton cv hai 7124 (gossypium barbadense) upon infection with verticillium dahliae two-dimensional differential gel electrophoresis and matrix-assisted laser desorption/ionization tandem tim ...201626869704
antisense expression of gossypium barbadense ugd6 in arabidopsis thaliana significantly alters cell wall composition.uridine diphosphate-glucose dehydrogenase (ugd, ec1.1.1.22 oxidizes udp-glc (udp-d-glucose) to udp-glca (udp-d-glucuronate), a critical precursor of cell wall polysaccharides. gbugd6 from gossypium barbadense is more highly expressed late in the elongation of cotton fibers (15 d post-anthesis (dpa)) and during the stage of secondary cell wall thickening (30 dpa). subcellular localization analysis in onion epidermis revealed that fluorescently labeled gbugd6 protein was distributed throughout the ...201626810898
deep-tissue confocal imaging of the central projections of ovipositor sensory afferents in the egyptian cotton leafworm, spodoptera littoralis.the pre-ovipositon behavior of moths is largely dependent upon the cues that a gravid female perceives while assessing potential oviposition sites. assessment of such sites is accomplished, at least in part, by mechanosensory and gustatory sensilla located on the ovipositor whose sensory neurons project into the terminal abdominal ganglion (tag). using anterograde backfill staining, confocal laser scanning microscopy, and three dimensional reconstruction, we traced and analyzed the central proje ...201626774745
genetic diversity of the two commercial tetraploid cotton species in the gossypium diversity reference set.a diversity reference set has been constructed for the gossypium accessions in the us national cotton germplasm collection to facilitate more extensive evaluation and utilization of accessions held in the collection. a set of 105 mapped simple sequence repeat markers was used to study the allelic diversity of 1933 tetraploid gossypium accessions representative of the range of diversity of the improved and wild accessions of g. hirsutum and g. barbadense. the reference set contained 410 g. barbad ...201626774060
breeding potential of introgression lines developed from interspecific crossing between upland cotton (gossypium hirsutum) and gossypium barbadense: heterosis, combining ability and genetic effects.upland cotton (gossypium hirstum l.), which produces more than 95% of the world natural cotton fibers, has a narrow genetic base which hinders progress in cotton breeding. introducing germplasm from exotic sources especially from another cultivated tetraploid g. barbadense l. can broaden the genetic base of upland cotton. however, the breeding potential of introgression lines (ils) in upland cotton with g. barbadense germplasm integration has not been well addressed. this study involved six ils ...201626730964
a ve homologous gene from gossypium barbadense, gbvdr3, enhances the defense response against verticillium dahliae.the tomato ve1 gene and several ve1 homologues are involved in the resistance to verticillium dahliae. here, we report on another ve homologous gene, gbvdr3, from a verticillium wilt-resistant cotton cultivar, gossypium barbadense hai7124, which has a 3207-bp region that encodes a predicted receptor-like protein. transient expression analyses indicated that gbvdr3 is localized in the plasma membrane, and virus-induced gene silencing of gbvdr3 compromised the resistance of hai7124 cotton to a def ...201626686282
the genome sequence of sea-island cotton (gossypium barbadense) provides insights into the allopolyploidization and development of superior spinnable fibres.gossypium hirsutum contributes the most production of cotton fibre, but g. barbadense is valued for its better comprehensive resistance and superior fibre properties. however, the allotetraploid genome of g. barbadense has not been comprehensively analysed. here we present a high-quality assembly of the 2.57 gigabase genome of g. barbadense, including 80,876 protein-coding genes. the double-sized genome of the a (or at) (1.50 gb) against d (or dt) (853 mb) primarily resulted from the expansion o ...201526634818
behavioral and metabolic effects of sublethal doses of two insecticides, chlorpyrifos and methomyl, in the egyptian cotton leafworm, spodoptera littoralis (boisduval) (lepidoptera: noctuidae).insecticides have long been used as the main method in limiting agricultural pests, but their widespread use has resulted in environmental pollution, development of resistances, and biodiversity reduction. the effects of insecticides at low residual doses on both the targeted crop pest species and beneficial insects have become a major concern. in particular, these low doses can induce unexpected positive (hormetic) effects on pest insects, such as surges in population growth exceeding what woul ...201626566611
rapid evolutionary divergence of gossypium barbadense and g. hirsutum mitochondrial genomes.the mitochondrial genome from upland cotton, g. hirsutum, was previously sequenced. to elucidate the evolution of mitochondrial genomic diversity within a single genus, we sequenced the mitochondrial genome from sea island cotton (gossypium barbadense l.).201526459858
overexpression of gbrlk, a putative receptor-like kinase gene, improved cotton tolerance to verticillium wilt.verticillium dahliae is a causative fungal pathogen and only a few genes have been identified that exhibit critical roles in disease resistance and few has shown positive effects on the resistance to verticillium wilt in transgenic cotton. we cloned a receptor-like kinase gene (gbrlk) induced by verticillium dahliae (vd) in the disease-resistant cotton gossypium barbadense cv. hai7124. northern blotting revealed that the gbrlk was induced by vd at 96 h after inoculation. the functional gbrlk is ...201526446555
gossypium barbadense genome sequence provides insight into the evolution of extra-long staple fiber and specialized metabolites.of the two cultivated species of allopolyploid cotton, gossypium barbadense produces extra-long fibers for the production of superior textiles. we sequenced its genome (ad)2 and performed a comparative analysis. we identified three bursts of retrotransposons from 20 million years ago (mya) and a genome-wide uneven pseudogenization peak at 11-20 mya, which likely contributed to genomic divergences. among the 2,483 genes preferentially expressed in fiber, a cell elongation regulator, pre1, is stri ...201526420475
molecular cloning and functional analysis of gbrvd, a gene in gossypium barbadense that plays an important role in conferring resistance to verticillium wilt.most of the disease resistance genes already characterized in plants encode nucleotide-binding site-leucine rich repeat (nbs-lrr) proteins that have key roles in resistance to verticillium dahliae. using a cdna library and race protocols, we cloned a coiled-coil (cc)-nbs-lrr-type gene, gbrvd, from a resistant tetraploid cotton species, gossypium barbadense (rvd=resistance to v. dahliae). we also applied rt-qpcr and vigs technologies to analyze how expression of gbrvd was induced upon attack by v ...201626407869
gbexpatr, a species-specific expansin, enhances cotton fibre elongation through cell wall restructuring.cotton provides us the most important natural fibre. high fibre quality is the major goal of cotton breeding, and introducing genes conferring longer, finer and stronger fibre from gossypium barbadense to gossypium hirsutum is an important breeding strategy. we previously analysed the g. barbadense fibre development mechanism by gene expression profiling and found two homoeologous fibre-specific α-expansins from g. barbadense, gbexpa2 and gbexpatr. gbexpa2 (from the dt genome) is a classical α-e ...201626269378
ethnobotanical study of medicinal plants by population of valley of juruena region, legal amazon, mato grosso, brazil.the use of medicinal plants for treatment, cure and prevention of diseases has been described by many people since time immemorial. because of this use, commercial and scientific interests have emerged, making it necessary to realize ethnobotanical surveys of medicinal plants species, which is important for subsequent chemical and pharmacological bioprospections.201526234177
molecular cloning, characterization and expression analysis of the gene encoding 1-deoxy-d-xylulose 5-phosphate reductoisomerase from aquilaria sinensis (lour.) gilg.the major constituents of agarwood oils are sesquiterpenes that are obtained from isoprenoid precursors through the plastidial methylerythritol phosphate (mep) pathway and the cytosolic mevalonate pathway. in this study, a novel full-length cdna of 1-deoxy-d-xylulose 5-phosphate reductoisomerase (dxr), which was the second key enzyme in the plastid mep pathway of sesquiterpenes biosynthesis was isolated from the stem of aquilaria sinensis (lour.) gilg by the methods of reverse transcription poly ...201526174671
the hairless stem phenotype of cotton (gossypium barbadense) is linked to a copia-like retrotransposon insertion in a homeodomain-leucine zipper gene (hd1).cotton (gossypium) stem trichomes are mostly single cells that arise from stem epidermal cells. in this study, a homeodomain-leucine zipper gene (hd1) was found to cosegregate with the dominant trichome locus previously designated as t1 and mapped to chromosome 6. characterization of hd1 orthologs revealed that the absence of stem trichomes in modern gossypium barbadense varieties is linked to a large retrotransposon insertion in the ninth exon, 2565 bp downstream from the initial codon in the a ...201526133897
identification of mirnas and their targets in cotton inoculated with verticillium dahliae by high-throughput sequencing and degradome analysis.micrornas (mirnas) are a group of endogenous small non-coding rnas that play important roles in plant growth, development, and stress response processes. verticillium wilt is a vascular disease in plants mainly caused by verticillium dahliae kleb., the soil-borne fungal pathogen. however, the role of mirnas in the regulation of verticillium defense responses is mostly unknown. this study aimed to identify new mirnas and their potential targets that are involved in the regulation of verticillium ...201526133244
metabolomic and transcriptomic insights into how cotton fiber transitions to secondary wall synthesis, represses lignification, and prolongs elongation.the morphogenesis of single-celled cotton fiber includes extreme elongation and staged cell wall differentiation. designing strategies for improving cotton fiber for textiles and other uses relies on uncovering the related regulatory mechanisms. in this research we compared the transcriptomes and metabolomes of two gossypium genotypes, gossypium barbadense cv phytogen 800 and g. hirsutum cv deltapine 90. when grown in parallel, the two types of fiber developed similarly except for prolonged fibe ...201526116072
diel trends in stomatal response to ozone and water deficit: a unique relationship of midday values to growth and allometry in pima cotton?plant responses to ozone (o3 ) and water deficit (wd) are commonly observed, although less is known about their interaction. stomatal conductance (gs ) is both an impact of these stressors and a protective response to them. stomatal closure reduces inward flux of o3 and outward flux of water. stomatal measurements are generally obtained at midday when gas exchange is maximal, but these may not be adequate surrogates for stomatal responses observed at other times of day, nor for non-stomatal resp ...201626031549
a cotton fiber-preferential promoter, pgbexpa2, is regulated by ga and aba in arabidopsis.pgbexpa2 (promoter of gbexpa2 ) was preferentially and strongly expressed during cotton fiber development, and the 461-bp pgbexpa2 fragment was essential for responding to exogenous ga and aba in arabidopsis. cotton fibers are highly elongated single-cell, unbranched and non-glandular seed trichomes. previous studies have reported that the transcript level of gbexpa2 is significantly up-regulated during fiber cell elongation, suggesting that gbexpa2 has an important function in fiber development ...201526001998
inhibition kinetics of acid and alkaline phosphatases by atrazine and methomyl pesticides.the main objective of this work was to investigate the kinetic characteristics of acid and alkaline phosphatases isolated from different sources and to study the effects of the herbicide atrazine and insecticide methomyl on the activity and kinetic properties of the enzymes. acid phosphatase (acp) was isolated from the tomato plant (solanum lycopersicum l. var. lycopersicum); alkaline phosphatase (alp) was isolated from two sources, including mature earthworms (aporrectodea caliginosa) and larva ...201525996812
molecular cloning of a dehydration-responsive protein gene (mrd22) from mulberry, and determination of abiotic stress patterns of mrd22 gene expression.a full-length cdna sequence coding for dehydration-responsive protein gene of mulberry tree, which we designated was mrd22 (genbank accession number: jq804833) was cloned based on mulberry expressed sequence tags (ests). mrd22 is 1503 bp long, contains a 334 bp 5'-utr (untranslated region) and a 563 bp 3'-utr, encodes 201 amino acids with a predicted molecular weight of 54.28 kda and an isoelectric point of 9.35. phylogenetic analysis based on mrd22 sequences from different species showed that m ...201525895340
molecular characterization of a transcriptionally active ty1/copia-like retrotransposon in gossypium.a transcriptionally active ty1/copia -like retrotransposon was identified in the genome of gossypium barbadense. the different heat activation of this element was observed in two tetraploid cotton species. most retrotransposons from plants are transcriptionally silent, or activated under certain conditions. only a small portion of elements are transcriptionally active under regular condition. a long terminal repeat (ltr) retrotransposon was isolated from the cultivated sea island cotton (h7124) ...201525693493
bioinformatics analysis of small rnas in pima (gossypium barbadense l.).small rnas (srnas) are ~20 to 24 nucleotide single-stranded rnas that play crucial roles in regulation of gene expression. in plants, srnas are classified into micrornas (mirnas), repeat-associated sirnas (ra-sirnas), phased sirnas (pha-sirnas), cis and trans natural antisense transcript sirnas (cis- and trans-nat sirnas). pima (gossypium barbadense l.) is one of the most economically important fiber crops, producing the best and longest spinnable fiber. although some mirnas are profiled in pima ...201525679373
a cotton myb transcription factor, gbmyb5, is positively involved in plant adaptive response to drought stress.drought stress negatively affects plant growth and limits plant productivity. genes functioning in plant responses to drought stress are essential for the development of drought-tolerant crops. here, we report that an r2r3-type myb transcription factor gene in gossypium barbadense, gbmyb5, confers drought tolerance in cotton and transgenic tobacco. virus-induced gene silencing of gbmyb5 compromised the tolerance of cotton plantlets to drought stress and reduced the post-rewatering water recovery ...201525657343
steam explosion distinctively enhances biomass enzymatic saccharification of cotton stalks by largely reducing cellulose polymerization degree in g. barbadense and g. hirsutum.in this study, steam explosion pretreatment was performed in cotton stalks, leading to 5-6 folds enhancements on biomass enzymatic saccharification distinctive in gossypium barbadense and gossypium hirsutum species. sequential 1% h2so4 pretreatment could further increase biomass digestibility of the steam-exploded stalks, and also cause the highest sugar-ethanol conversion rates probably by releasing less inhibitor to yeast fermentation. by comparison, extremely high concentration alkali (16% na ...201525656866
assessing the total factor productivity of cotton production in egypt.the main objective of this paper is to decompose the productivity growth of egyptian cotton production. we employ the stochastic frontier approach and decompose the changes in total factor productivity (ctfp) growth into four components: technical progress (tp), changes in scale component (csc), changes in allocative efficiency (cae), and changes in technical efficiency (cte). considering a situation of scarce statistical information, we propose four alternative empirical models, with the purpos ...201525625318
identification and characterization of a fatty acyl reductase from a spodoptera littoralis female gland involved in pheromone biosynthesis.fatty acyl-coa reductases (fars), the enzymes that catalyse reduction of a fatty acyl-coa to the corresponding alcohol in insect pheromone biosynthesis, are postulated to play an important role in determining the proportion of each component in the pheromone blend. for the first time, we have isolated and characterized from the egyptian cotton leaf worm spodoptera littoralis (lepidoptera: noctuidae) a far cdna (slit-far1), which appeared to be expressed only in the pheromone gland and was undete ...201525558806
longitudinal assessment of occupational exposures to the organophosphorous insecticides chlorpyrifos and profenofos in egyptian cotton field workers.chlorpyrifos (cpf) and profenofos (pff) are organophosphorus (op) insecticides that are applied seasonally in egypt to cotton fields. urinary trichloro-2-pyridinol (tcpy), a specific cpf metabolite, and 4-bromo-2-chlorophenol (bcp), a specific pff metabolite, are biomarkers of exposure, while inhibition of blood butyrylcholinesterase (bche) and acetylcholinesterase (ache) activities are effect biomarkers that may be associated with neurotoxicity. urinary tcpy and bcp and blood bche and ache acti ...201525466362
cotton wrky1 mediates the plant defense-to-development transition during infection of cotton by verticillium dahliae by activating jasmonate zim-domain1 expression.plants have evolved an elaborate signaling network to ensure an appropriate level of immune response to meet the differing demands of developmental processes. previous research has demonstrated that della proteins physically interact with jasmonate zim-domain1 (jaz1) and dynamically regulate the interaction of the gibberellin (ga) and jasmonate (ja) signaling pathways. however, whether and how the jaz1-della regulatory node is regulated at the transcriptional level in plants under normal growth ...201425301887
cotton fiber elongation network revealed by expression profiling of longer fiber lines introgressed with different gossypium barbadense chromosome segments.cotton fiber, a highly elongated, thickened single cell of the seed epidermis, is a powerful cell wall research model. fiber length, largely determined during the elongation stage, is a key property of fiber quality. several studies using expressed sequence tags and microarray analysis have identified transcripts that accumulate preferentially during fiber elongation. to further show the mechanism of fiber elongation, we used digital gene expression tag profiling to compare transcriptome data fr ...201425273845
constructing a high-density linkage map for gossypium hirsutum × gossypium barbadense and identifying qtls for lint percentage.to introgress the good fiber quality and yield from gossypium barbadense into a commercial upland cotton variety, a high-density simple sequence repeat (ssr) genetic linkage map was developed from a bc1 f1 population of gossypium hirsutum × gossypium barbadense. the map comprised 2,292 loci and covered 5115.16 centimorgan (cm) of the cotton ad genome, with an average marker interval of 2.23 cm. of the marker order for 1,577 common loci on this new map, 90.36% agrees well with the marker order on ...201525263268
characterization of the global transcriptome for cotton (gossypium hirsutum l.) anther and development of ssr marker.cotton is an important fiber plant, and it's attractive to elucidate the molecular mechanism of anther development due to the close relationship between the anther fertility and boll-setting, and also fiber yield. in the present paper, 47.2 million paired-end reads with average length of 82.87 bp from the anthers of tm-1 (gossypium hirsutum l.), a genetic standard line, were generated through transcriptome sequencing, and 210,965 unigenes of more than 100 bp were obtained. blast, kegg, cog, and ...201425178523
direct visualization of horizontal gene transfer in cotton plants.plant mitochondrial and chloroplast genes that underwent horizontal transfer have been identified by parasite and grafting systems, respectively. here, we directly observed 3 horizontal gene transfer (hgt) events in the 45 second axillary shoots of grafted cotton plants (gossypium barbadense and gossypium hirsutum) after extirpating the first axillary bud. the second axillary shoots showed phenotypic variations in cotton flowers and seeds that were evidence of spontaneous development from cells ...201425160847
small rna and degradome profiling reveals a role for mirnas and their targets in the developing fibers of gossypium barbadense.micrornas (mirnas) are 20-24 nucleotide non-coding small rnas that play important roles in plant development. the stages of cotton fiber development include initiation, elongation, secondary wall thickening (swt) and maturation. we constructed seven fiber rna libraries representing the initiation, elongation and swt stages. in total, 47 conserved mirna families and seven candidate mirnas were profiled using small rna sequencing. northern blotting and real-time polymerase chain reaction (pcr) ana ...201425131375
quantitative and ultrastructural changes in the haemocytes of spodoptera littoralis (boisd.) treated individually or in combination with spodoptera littoralis multicapsid nucleopolyhedrovirus (splimnpv) and azadirachtin.the total haemocyte count (thc) and the possible ultrastructural alterations induced in the haemocytes of the fourth larval instars of the egyptian cotton leafworm, spodoptera littoralis (boisd.) (lepidoptera: noctuidae), 96 h post-feeding on a semi-synthetic diet, treated with the lc50 of spodoptera littoralis multicapsid nucleopolyhedrovirus (splimnpv) and the lc50 of azadirachtin alone, and the lc25 of splimnpv combined with the lc25 of azadirachtin were studied and compared to the control. s ...201425041832
transcriptomic analysis of fiber strength in upland cotton chromosome introgression lines carrying different gossypium barbadense chromosomal segments.fiber strength is the key trait that determines fiber quality in cotton, and it is closely related to secondary cell wall synthesis. to understand the mechanism underlying fiber strength, we compared fiber transcriptomes from different g. barbadense chromosome introgression lines (csils) that had higher fiber strengths than their recipient, g. hirsutum acc. tm-1. a total of 18,288 differentially expressed genes (degs) were detected between csil-35431 and csil-31010, two csils with stronger fiber ...201424762562
molecular cloning and characterization of enhanced disease susceptibility 1 (eds1) from gossypium barbadense.arabidopsis enhanced disease susceptibility 1 (eds1) plays an important role in plant defense against biotrophic and necrotrophic pathogens. the necrotrophic pathogen verticillium dahliae infection of gossypium barbadense could lead to verticillium wilt which seriously reduces the cotton production. here, we cloned and characterized a g. barbadense homolog of eds1, designated as gbeds1. the full-length cdna of the gbeds1 gene was obtained by the technique of rapid-amplification of cdna ends. the ...201424570021
the genetics of blackarm resistance. viii. gossypium barbadense. 195024538925
sex pheromone of egyptian cotton leafworm (spodoptera littoralis). its chemical transformation under field conditions. 198124420691
analysis of sea-island cotton and upland cotton in response to verticillium dahliae infection by rna sequencing.cotton verticillium wilt is a serious soil-borne vascular disease that causes great economic loss each year. however, due to the lack of resistant varieties of upland cotton, the molecular mechanisms of resistance to this disease, especially to the pathogen verticillium dahliae, remain unclear.201324314117
insect antifeedant activity of clerodane diterpenoids against larvae ofspodoptera littoralis (boisd.) (lepidoptera).antifeedant activities of nine clerodane diterpenoids, isolated in this laboratory from different species ofajuga plants, have been studied against larvae of egyptian cotton leafwormspodoptera littoralis (boisd.) (lepidoptera) by application of the leaf disk method. evaluation of activity was carried out by calculating, at different time intervals, the feeding ratio (fr) from the relationship between the consumed areas of treated disks (ctd) and control disks (ccd); for comparison purposes a fr5 ...198524311185
ssr marker-assisted improvement of fiber qualities in gossypium hirsutum using g. barbadense introgression lines.this study demonstrates the first practical use of csils for the transfer of fiber quality qtls into upland cotton cultivars using ssr markers without detrimentally affecting desirable agronomic characteristics. gossypium hirsutum is characterized by its high lint production and medium fiber quality compared to extra-long staple cotton g. barbadense. transferring valuable traits or genes from g. barbadense into g. hirsutum is a promising but challenging approach through a traditional interspecif ...201424306319
chemical protection of pheromones containing an internal conjugated diene system from isomerization and oxidation.conjugated diene systems are common in natural products, including pheromones. the systems are sensitive to heat, light, and oxygen, among other things. they can be protected by antioxidants and uv absorbers, which slow downcis-trans isomerization and oxidation. three sex pheromones (one as an analog) containingz,e, e,z, ande,e units were studied: (z,e)-9,11-c14oac, (e,z)-7,9-c12oac, and (e,e)-10,12-c16oac. the uv absorber 2-hydroxy-4-methoxybenzophenone and the antioxidants bht and bha were fou ...198824276497
investigation of oviposition deterrent in larval frass ofspodoptera littoralis (boisd.).previous experiments demonstrated an oviposition-deterring effect of larval frass in the egyptian cotton leaf worm,spodoptera littoralis (boisd.). in this study, females were shown to perceive the oviposition-deterring substance(s) with their antennae. during dark, airtight, and cold (-10 °c) storage, the deterrent was persistent for at least 395 days. on the other hand, larval frass retained its activity for only two days when applied to cotton leaves. the deterrent activity of frass was indepe ...198924271895
allozyme evidence for the origin and diversification of gossypium barbadense l.gossypium barbadense l. is a commercially important cotton species of tropical south american origin presently grownin many regions of the world. the species is morphologically diverse, consisting of a wide range of wild (or feral), commensal, landrace, and highly improvedcommercial forms. we performed allozyme analysis on 153 accessions representing the spectrum of g. barbadense diversityto ascertain the geographic origin of the species, its patterns of diffusion subsequent to domestication, an ...199024226459
regeneration of gossypium hirsutum and g. barbadense from shoot apex tissues for transformation.a method of regenerating cotton plants from the shoot apical meristem of seedlings was developed for use with particle gun and agrobacterium-mediated transformation. this method was developed to circumvent the problems of genotype restriction and chromosomal damage frequently encountered in cotton regeneration in tissue culture through somatic embryogenesis. in this procedure, the cells of the shoot meristem are targeted for transformation. normal and fertile plants of gossypium barbadense pima ...199124226156
photosynthetic electron transport in thylakoids from cotton cultivars (gossypium sp.) differing in tolerance to prometryn.a method to determine photosynthetic electron transport in thylakoid membranes is described for gossypium barbadense (cv. pima s-7) and g. hirsutum (cv. dp 5415). these cultivars differed markedly in tolerance to prometryn, a ps ii inhibitor. the rates of photosynthetic electron transport obtained were 245 μmole oxygen mg(-1) chl h(1). plant age and leaf size influenced the activity of the thylakoid preparations. thylakoids from leaves of plants 24 to 37 d and 50-70 mm in diameter had the highes ...199624178285
the distribution of gossypium hirsutum chromatin in g. barbadense germ plasm: molecular analysis of introgressive plant breeding.cotton is unusual among major crop plants in that two cross-fertile species are widely cultivated for a common economic product, fiber. both historical evidence and classical genetic studies suggest that many improved forms of gossypium barbadense ("sea island", "egyptian", and "pima" cottons) may include chromatin derived from g. hirsutum. using 106 restriction fragment length polymorphism (rflp) loci well distributed across the cotton genome, we revealed the amount and genomic distribution of ...199524170011
modulation of reproductive behaviors by non-host volatiles in the polyphagous egyptian cotton leafworm, spodoptera littoralis.in order to locate mates, food, and oviposition sites, insects mainly rely on volatile cues released by their sexual partners, food sources, and host and non-host plants. calling, mating, and oviposition behaviors, as well as fecundity and longevity, of newly emerged spodoptera littoralis (bois.) moths were recorded in the presence of volatiles from leaves of a host plant, gossypium hirsutum (cotton) and two non-host plants, adhatoda vasica (av) or picea abies (spruce), either alone or in host/n ...201324105603
association analysis of yield and fiber quality traits in gossypium barbadense with ssrs and sraps.cotton is an important cash crop. mining for quantitative trait loci related to yield and fiber quality traits using association analysis has many advantages for cotton research. in this study, 170 simple sequence repeats (ssrs) and 258 sequence-related amplified polymorphisms (sraps) were used to analyze the association of 3 yield component traits and 5 fiber quality traits of 55 gossypium barbadense accessions in 2009 and 2010. principal component analysis of ssrs and sraps showed 3 and 2 subg ...201324065676
transcriptome profiling of gossypium barbadense inoculated with verticillium dahliae provides a resource for cotton improvement.verticillium wilt, caused by the fungal pathogen verticillium dahliae, is the most severe disease in cotton (gossypium spp.), causing great lint losses worldwide. disease management could be achieved in the field if genetically improved, resistant plants were used. however, the interaction between v. dahliae and cotton is a complicated process, and its molecular mechanism remains obscure. to understand better the defense response to this pathogen as a means for obtaining more tolerant cultivars, ...201324053558
functional analysis of the seed coat-specific gene gbmyb2 from cotton.myb transcription factors are essential for cotton fiber development. we isolated the r2r3-myb gene gbmyb2 from gossypium barbadense. rna in situ hybridization analysis showed that gbmyb2 is mainly expressed in the outer integuments of cotton ovules and in elongating fibers. gbmyb2 expression increased throughout fiber initiation and during the elongation stage. the expression level of gbmyb2 was higher in the gossypium hirsutum cultivar xu142 than in the xu142 (fl) mutant. overexpression of gbm ...201324036393
development of agrobacterium-mediated virus-induced gene silencing and performance evaluation of four marker genes in gossypium barbadense.gossypiumbarbadense is a cultivated cotton species and possesses many desirable traits, including high fiber quality and resistance to pathogens, especially verticilliumdahliae (a devastating pathogen of gossypium hirsutum, the main cultivated species). these elite traits are difficult to be introduced into g. hirsutum through classical breeding methods. in addition, genetic transformation of g. barbadense has not been successfully performed. it is therefore important to develop methods for eval ...201324023833
proteomic and virus-induced gene silencing (vigs) analyses reveal that gossypol, brassinosteroids, and jasmonic acid contribute to the resistance of cotton to verticillium dahliae.verticillium wilt causes massive annual losses of cotton yield, but the mechanism of cotton resistance to verticillium dahliae is complex and poorly understood. in this study, a comparative proteomic analysis was performed in resistant cotton (gossypium barbadense cv7124) on infection with v. dahliae. a total of 188 differentially expressed proteins were identified by mass spectrometry (maldi-tof/tof) analysis and could be classified into 17 biological processes based on gene ontology annotation ...201324019146
genetical and biological control of cotton ashy stem caused by macrophomina phaseolina in outdoor pot experiment.two outdoor pot experiments were carried out to evaluate the reaction of 11 commercial egyptian cotton cultivars macrophomina phaseolina, the incitant of ashy stem in cotton and to evaluate the antagonistic ability of 27 isolates of trichoderma sp. against pathogen cotton cultivars giza 85, giza 87, giza 89 and giza 90 were resistant to m. phaseolina because both survival and plant height of these cultivars was not affected when the soil was infested with the pathogen. none of the cultivars were ...201023961071
distribution of neuropeptides in the antennal lobes of male spodoptera littoralis.olfaction is an important sensory modality that regulates a plethora of behavioural expressions in insects. processing of olfactory information takes place in the primary olfactory centres of the brain, namely the antennal lobes (als). neuropeptides have been shown to be present in the olfactory system of various insect species. in the present study, we analyse the distribution of tachykinin, fmrfamide-related peptides, allatotropin, allatostatin, myoinhibitory peptides and sifamide in the al of ...201323955643
Displaying items 1 - 100 of 304