| investigation on the effect of different levels of dried sweet orange (citrus sinensis) pulp on performance, carcass characteristics and physiological and biochemical parameters in broiler chicken. | utilization of agricultural by-products in animal nutrition is a matter of great concern. dried sweet orange (citrus sinensis) pulp (dcsp) is a potential source of valuable nutrients and natural antioxidants for poultry feed. in the experiment, a feeding trial was conducted in order to investigate the effect of different levels of dried orange residues in diet on broiler growth performance, carcass characteristics, blood metabolites, humoral immunity, and cecum microbial population. a total of 2 ... | 2015 | 25737644 |
| correction to in vitro bioaccessibility of carotenoids, flavonoids, and vitamin c from differently processed oranges and orange juices [citrus sinensis (l.) osbeck]. | | 2015 | 25714679 |
| genomewide analysis of small rnas in nonembryogenic and embryogenic tissues of citrus: microrna- and sirna-mediated transcript cleavage involved in somatic embryogenesis. | somatic embryogenesis (se) is a process of somatic cells becoming dedifferentiated and generating embryos. se has been widely used in biotechnology as a powerful way of regeneration and a model system for studying plant embryogenesis, but the controlling mechanisms of se are far from clear. here, we show the genomewide profiles of mirnas/sirnas and their target genes in nonembryogenic and embryogenic tissues of 'valencia' sweet orange. by high-throughput sequencing (hts) of small rnas and rna de ... | 2015 | 25615015 |
| clinical evaluation of moro (citrus sinensis (l.) osbeck) orange juice supplementation for the weight management. | in the last years, several studies have recently evaluated the beneficial effects of red orange juice (citrus sinensis (l.) osbeck) and its active components in weight management and obesity. moro orange is a cultivar of red orange, particularly rich in active compounds such as anthocyanins, hydroxycinnamic acids, flavone glycosides and ascorbic acid, which displays anti-obesity effects in in vitro and in vivo studies. in this clinical study, the effect of a moro juice extract (morosil(®), 400 m ... | 2015 | 25588369 |
| comparative transcriptome analyses between a spontaneous late-ripening sweet orange mutant and its wild type suggest the functions of aba, sucrose and ja during citrus fruit ripening. | a spontaneous late-ripening mutant of 'jincheng' (c. sinensis l. osbeck) sweet orange exhibited a delay of fruit pigmentation and harvesting. in this work, we studied the processes of orange fruit ripening through the comparative analysis between the jincheng mutant and its wild type. this study revealed that the fruit quality began to differ on 166th days after anthesis. at this stage, fruits were subjected to transcriptome analysis by rna sequencing. 13,412 differentially expressed unigenes (d ... | 2014 | 25551568 |
| in vitro bioaccessibility of carotenoids, flavonoids, and vitamin c from differently processed oranges and orange juices [citrus sinensis (l.) osbeck]. | carotenoid, flavonoid, and vitamin c concentrations were determined in fresh orange segments and a puree-like homogenate derived thereof, as well as freshly squeezed, flash-pasteurized, and pasteurized juices. lutein and β-cryptoxanthin were slightly degraded during dejuicing, whereas β-carotene levels were retained. vitamin c levels remained unaffected, whereas flavonoid levels decreased 8-fold upon juice extraction, most likely due to the removal of flavonoid-rich albedo and juice vesicles. li ... | 2015 | 25539394 |
| carbon isotope ratios of selected volatiles in citrus sinensis and in orange-flavoured food. | twenty genuine samples of industrially cold-pressed sweet orange essential oils, were analysed by gas chromatography-combustion-isotope ratio mass spectrometry to determine the values of the carbon isotope ratios (δ(13)c(vpdb)) of selected volatiles and assess the corresponding range of authenticity. successively, four commercial orange-flavoured products were analysed under identical conditions to evaluate the authenticity of the orange flavour. the samples were extracted by solid-phase microex ... | 2015 | 25475589 |
| degradation kinetic modelling of ascorbic acid and colour intensity in pasteurised blood orange juice during storage. | the stability of ascorbic acid and colour intensity in pasteurised blood orange juice (citrus sinensis [l.] osbeck) during one month of storage was investigated at 4-37 °c. the effects of ascorbic acid fortification (at 100, 200 mg l(-1)) and deaeration, temperature/time storage on the kinetic behaviour were determined. ascorbic acid was monitored by hplc-dad and colour intensity by spectrophotometric measurements. degradation kinetics were best fitted by first-order reaction models for both asc ... | 2015 | 25466074 |
| different citrus rootstocks present high dissimilarities in their antioxidant activity and vitamins content according to the ripening stage. | "lane late" sweet orange grafted on six different citrus rootstocks and grown in the guadalquivir valley (seville, spain) were picked at different ripening stages in two consecutive seasons to characterize their antioxidant activity (free radicals scavenging activity, reducing power and lipid peroxidation inhibition) and quantify their main antioxidant compounds (vitamin e and vitamin c). linear discriminant analysis and 2-way anova were applied to compare the effects induced by citrus rootstock ... | 2015 | 25462974 |
| incidence of 'candidatus liberibacter asiaticus'-infected plants among citrandarins as rootstock and scion under field conditions. | huanglongbing (hlb), caused by the bacterium 'candidatus liberibacter' spp., is currently one of the most serious diseases of citrus plants and has caused substantial economic losses. thus far, there is no source of genetic resistance to hlb in the genus citrus or its relatives. however, several studies have reported poncirus trifoliata and some of its hybrids to be more tolerant to the disease. the main objective of this study was to report differences in the incidence of 'ca. l. asiaticus' inf ... | 2015 | 25423067 |
| genome-wide classification and evolutionary and expression analyses of citrus myb transcription factor families in sweet orange. | myb family genes are widely distributed in plants and comprise one of the largest transcription factors involved in various developmental processes and defense responses of plants. to date, few myb genes and little expression profiling have been reported for citrus. here, we describe and classify 177 members of the sweet orange myb gene (csmyb) family in terms of their genomic gene structures and similarity to their putative arabidopsis orthologs. according to these analyses, these csmybs were c ... | 2014 | 25375352 |
| cdna-aflp analysis reveals the adaptive responses of citrus to long-term boron-toxicity. | boron (b)-toxicity is an important disorder in agricultural regions across the world. seedlings of 'sour pummelo' (citrus grandis) and 'xuegan' (citrus sinensis) were fertigated every other day until drip with 10 μm (control) or 400 μm (b-toxic) h3bo3 in a complete nutrient solution for 15 weeks. the aims of this study were to elucidate the adaptive mechanisms of citrus plants to b-toxicity and to identify b-tolerant genes. | 2014 | 25348611 |
| involvements of pcd and changes in gene expression profile during self-pruning of spring shoots in sweet orange (citrus sinensis). | citrus shoot tips abscise at an anatomically distinct abscission zone (az) that separates the top part of the shoots into basal and apical portions (citrus self-pruning). cell separation occurs only at the az, which suggests its cells have distinctive molecular regulation. although several studies have looked into the morphological aspects of self-pruning process, the underlying molecular mechanisms remain unknown. | 2014 | 25308090 |
| genome-wide identification and phylogenetic analysis of the ap2/erf gene superfamily in sweet orange (citrus sinensis). | sweet orange (citrus sinensis) plays an important role in the economy of more than 140 countries, but it is grown in areas with intermittent stressful soil and climatic conditions. the stress tolerance could be addressed by manipulating the ethylene response factor (erf) transcription factors because they orchestrate plant responses to environmental stress. we performed an in silico study on the erfs in the expressed sequence tag database of c. sinensis to identify potential genes that regulate ... | 2014 | 25299098 |
| evaluation of the anti-proliferative and cytostatic effect of citrus sinensis (orange) fruit juice. | this work has been designed to evaluate the anti-proliferative and cytostatic effects of citrus sinensis (orange) fruit juice on rapidly proliferating cells. | 2014 | 25298937 |
| phenology of asian citrus psyllid (hemiptera: liviidae) and associated parasitoids on two species of citrus, kinnow mandarin and sweet orange, in punjab pakistan. | the population phenology of asian citrus psyllid, diaphorina citri kuwayama, was monitored weekly for 110 wk on two species of citrus, kinnow mandarin and sweet orange, at two different research sites in faisalabad, punjab pakistan. citrus flush growth patterns were monitored and natural enemy surveys were conducted weekly. flush patterns were similar for kinnow and sweet orange. however, flush on sweet orange was consistently more heavily infested with asian citrus psyllid than kinnow flush; de ... | 2014 | 25198345 |
| genome wide characterization of short tandem repeat markers in sweet orange (citrus sinensis). | sweet orange (citrus sinensis) is one of the major cultivated and most-consumed citrus species. with the goal of enhancing the genomic resources in citrus, we surveyed, developed and characterized microsatellite markers in the ≈347 mb sequence assembly of the sweet orange genome. a total of 50,846 ssrs were identified with a frequency of 146.4 ssrs/mbp. dinucleotide repeats are the most frequent repeat class and the highest density of ssrs was found in chromosome 4. ssrs are non-randomly distrib ... | 2014 | 25148383 |
| genome-wide comparison of micrornas and their targeted transcripts among leaf, flower and fruit of sweet orange. | in plants, micrornas (mirnas) regulate gene expression mainly at the post-transcriptional level. previous studies have demonstrated that mirna-mediated gene silencing pathways play vital roles in plant development. here, we used a high-throughput sequencing approach to characterize the mirnas and their targeted transcripts in the leaf, flower and fruit of sweet orange. | 2014 | 25142253 |
| proteomic and metabolomic profiling of valencia orange fruit after natural frost exposure. | the aim of this study was to evaluate the response of orange fruit (citrus sinensis var. valencia late) to freezing stress in planta, both immediately after the natural event and after a week, in order to understand the biochemical and molecular basis of the changes that later derive in internal and external damage symptoms. using two-dimensional differential gel electrophoresis to analyze exposed and non-exposed fruit, 27 differential protein spots were detected in juice sacs and flavedo, among ... | 2015 | 25132553 |
| prediction and functional analysis of the sweet orange protein-protein interaction network. | sweet orange (citrus sinensis) is one of the most important fruits world-wide. because it is a woody plant with a long growth cycle, genetic studies of sweet orange are lagging behind those of other species. | 2014 | 25091279 |
| phytochemical analysis and radical scavenging profile of juices of citrus sinensis, citrus anrantifolia, and citrus limonum. | the aim of the current investigation was to identify bioactive secondary metabolites including phenols, tannins, flavonoids, terpinedes, and steroids and compare the phytochemical analysis and antioxidant profile of the juice extracted from the fruits of citrus sinensis, citrus anrantifolia, and citrus limonum. | 2014 | 25024932 |
| comparative effect of citrus sinensis and carbimazole on serum t4, t3 and tsh levels. | there are previous independent reports on the anti-thyroid property of citrus sinensis. this isoflavones and phenolic acid-rich natural agent is widely consumed as dietary supplement, thus the need to investigate its comparative effect with a standard anti-thyroid drug on t4, t3 and thyroid stimulating hormone (tsh) levels. | 2014 | 25013255 |
| [citrus boron nutrient level and its impact factors in the three gorges reservoir region of chongqing, china]. | to investigate the level of boron nutrient in citrus and its impact factors, a total of 954 citrus leaf samples and 302 soil samples were collected from representative orchards in the 12 main citrus production counties in the three gorges reservoir region of chongqing to determine the boron content in citrus leaves, as well as the relationships between leaf boron content with soil available boron content, soil ph value, cultivar, rootstock and the age of tree. results indicated that the leaf sam ... | 2014 | 25011290 |
| genome-wide analysis of the r2r3-myb transcription factor gene family in sweet orange (citrus sinensis). | myb transcription factor represents one of the largest gene families in plant genomes. sweet orange (citrus sinensis) is one of the most important fruit crops worldwide, and recently the genome has been sequenced. this provides an opportunity to investigate the organization and evolutionary characteristics of sweet orange myb genes from whole genome view. in the present study, we identified 100 r2r3-myb genes in the sweet orange genome. a comprehensive analysis of this gene family was performed, ... | 2014 | 25008995 |
| in vitro studies of a distillate of rectified essential oils on sinonasal components of mucociliary clearance. | herbal remedies predate written history and continue to be used frequently for many common ailments. the essential oil mixture standardized is a phytopharmaceutical with a distillate of a mixture of rectified essential oils of eucalyptus, sweet orange, myrtle, and lemon as active ingredients used to treat respiratory diseases such as bronchitis and rhinosinusitis. we evaluated the pharmacologic effects of a distillate of rectified essential oils standardized on primary human upper respiratory ep ... | 2014 | 24980236 |
| reduction of non-enzymatic browning of orange juice and semi-concentrates by removal of reaction substrate. | a study was conducted to standardize the technology for the removal of amino acids (one of the browning reaction substrates) from sweet orange cv. malta common juice to reduce colour and quality deterioration in single strength juice and during subsequent concentration. juice of sweet orange (citrus sinensis) cv. malta common fruits was extracted by screw type juice extractor, preserved in 500 ppm so2 and clarified by using "pectinase ccm" enzyme (0.2% for 2 h at 50 ± 2 °c). for removal of amino ... | 2014 | 24966423 |
| effects of boron deficiency on major metabolites, key enzymes and gas exchange in leaves and roots of citrus sinensis seedlings. | boron (b) deficiency is a widespread problem in many crops, including citrus. the effects of b-deficiency on gas exchange, carbohydrates, organic acids, amino acids, total soluble proteins and phenolics, and the activities of key enzymes involved in organic acid and amino acid metabolism in 'xuegan' [citrus sinensis (l.) osbeck] leaves and roots were investigated. boron-deficient leaves displayed excessive accumulation of nonstructural carbohydrates and much lower co2 assimilation, demonstrating ... | 2014 | 24957048 |
| [preliminary analysis of bitter substances in spica of prunella vulgaris]. | volatile oil components and the contents and types of amino acid in spica of prunella vulgaris were analysed by gc-ms and amino acid analyzer. esters, fatty acids, aromatic hydrocarbon, ketone and several alcohol compounds were identified by mass spectrum comparison. in these ingredients, beta-ionone smelled aroma of cedar, raspberry, nerolidol showed weak sweet soft orange blossom flavor, neroli tasted sweet and fresh, nerolidol tasted sweet with light aroma of wood, hexadecanal showed a weak a ... | 2014 | 24946541 |
| effect of second cooling on the chemical components of essential oils from orange peel (citrus sinensis). | a second cooling was added to the oil collectors of an improved clevenger-type apparatus (ict) to investigate the thermal reaction of essential oils from orange peel compared to a traditional clevenger-type apparatus (ct). the results demonstrated the yield rate of essential oil from ict was significantly higher (p < 0.05) than that from ct. the major components of the essential oils consisted of monoterpenes, such as d-limonene, β-myrcene, β-pinene, γ-terpinene, α-pinene. interestingly, ict pre ... | 2014 | 24945493 |
| protective effect of red orange extract supplementation against uv-induced skin damages: photoaging and solar lentigines. | exposure of the skin to solar ultraviolet (uv) radiations causes important oxidative damages that result in clinical and hystopathological changes, contributing to premature skin aging. hyperpigmented lesions, also known as age spots, are one of the most visible alterations in skin photoaging. skin is naturally equipped with antioxidant systems against uv-induced ros generation; however, these antioxidant defenses are not completely efficient during exposure to sunlight. oral antioxidants are ab ... | 2014 | 24910279 |
| oviposition deterrent and ovicidal activities of seven herbal essential oils against female adults of housefly, musca domestica l. | the oviposition deterrent and ovicidal of seven herbal essential oils derived from citrus sinensis, cymbopogon citratus, eucalyptus glubulus, illicium verum, lavandula angustifolia, mentha piperita, and zingiber cussumunar were assessed against the gravid female of housefly, musca domestica l., under laboratory conditions and compared with commercial insecticide (10% w/v cypermethrin). they were assayed at three concentrations (1.0, 5.0, and 10.0%) where plastic cups containing 1 ml of desired o ... | 2014 | 24894080 |
| identification of boron-deficiency-responsive micrornas in citrus sinensis roots by illumina sequencing. | boron (b)-deficiency is a widespread problem in many crops, including citrus. micrornas (mirnas) play important roles in nutrient deficiencies. however, little is known on b-deficiency-responsive mirnas in plants. in this study, we first identified mirnas and their expression pattern in b-deficient citrus sinensis roots by illumina sequencing in order to identify mirnas that might be involved in the tolerance of plants to b-deficiency. | 2014 | 24885979 |
| influence of crop load on the expression patterns of starch metabolism genes in alternate-bearing citrus trees. | the fruit is the main sink organ in citrus and captures almost all available photoassimilates during its development. consequently, carbohydrate partitioning and starch content depend on the crop load of citrus trees. nevertheless, little is known about the mechanisms controlling the starch metabolism at the tree level in relation to presence of fruit. the aim of this study was to find the relation between the seasonal variation of expression and activity of the genes involved in carbon metaboli ... | 2014 | 24747724 |
| a transcriptional approach to unravel the connection between phospholipases a₂ and d and aba signal in citrus under water stress. | the effect of water stress on the interplay between phospholipases (pl) a2 and d and aba signalling was investigated in fruit and leaves from the sweet orange navelate and its fruit-specific aba-deficient mutant pinalate by studying simultaneously expression of 5 pld and 3 pla2-encoding genes. in general, expression levels of pld-encoding genes were higher at harvest in the flavedo (coloured outer part of the peel) from pinalate. moreover, a higher and transient increase in expression of cspldα, ... | 2014 | 24713122 |
| study of production and pyrolysis characteristics of sweet orange flavor-β-cyclodextrin inclusion complex. | flavor plays an important role and has been widely used in foods. encapsulation can prevent the loss of volatile aromatic ingredients, provide protection and enhance the stability of the flavor. kinetic and thermodynamic parameters are helpful in understanding the mechanism of molecular recognition between hosts and guests. this work focused on the study of production of a sweet orange flavor-β-cyclodextrin (cd) inclusion complex, and investigated the combination of flavor and β-cd by thermograv ... | 2014 | 24708955 |
| study on essential oils from four species of zhishi with gas chromatography-mass spectrometry. | citrus fruits are widely used as food and or for medicinal purposes, and they contain a host of active substances that contribute to health. the immature fruits of citrus sinensis osbeck and its cultivars (cs), c. junos sieb. ex tanaka (cj), c. aurantium l. and its cultivars (ca) and poncirus trifoliate raf. (pt) are the most commonly used medicinal herbs in traditional chinese medicine, called zhishi. and their mature fruits can be used as food. | 2014 | 24708882 |
| impact of sensory feed additives on feed intake, feed preferences, and growth of female piglets during the early postweaning period. | our study aimed at investigating the effect of feed supplementation, from weaning, with 3 sensory feed additives (fa1, fa2, and fa3) on feed preferences, feed intake, and growth of piglets. the fa1 contained extract of stevia rebaudiana (10 to 20%), extract of high-saponin plants (5 to 10%), and excipients (70 to 85%), the fa2 was mainly composed of a natural extract of citrus sinensis (60 to 80%), and the fa3 was made of a blend of extracts of hot-flavored spices (5 to 15%) and excipients (85 t ... | 2014 | 24668952 |
| physicochemical and functional properties of micronized jincheng orange by-products (citrus sinensis osbeck) dietary fiber and its application as a fat replacer in yogurt. | orange by-products from juice extraction are generally discarded or used in animal feed due to their low market value. however, orange by-products show potential as dietary fiber (df) and fat replacers in products such as yogurt. this study assessed the benefits of using orange by-products in df-enriched materials such as df powders (op) and micronized df with ball-milling (mdf). the study also investigated the effects of adding different levels of op and mdf on the quality of low-fat yogurt. re ... | 2014 | 24625022 |
| temporal progression of 'candidatus liberibacter asiaticus' infection in citrus and acquisition efficiency by diaphorina citri. | over the last decade, the plant disease huanglongbing (hlb) has emerged as a primary threat to citrus production worldwide. hlb is associated with infection by phloem-limited bacteria ('candidatus liberibacter' spp.) that are transmitted by the asian citrus psyllid, diaphorina citri. transmission efficiency varies with vector-related aspects (e.g., developmental stage and feeding periods) but there is no information on the effects of host-pathogen interactions. here, acquisition efficiency of 'c ... | 2014 | 24620723 |
| an integrative analysis of the transcriptome and proteome of the pulp of a spontaneous late-ripening sweet orange mutant and its wild type improves our understanding of fruit ripening in citrus. | fruit ripening is a complex, genetically programmed process that occurs in conjunction with the differentiation of chloroplasts into chromoplasts and involves changes to the organoleptic properties of the fruit. in this study, an integrative analysis of the transcriptome and proteome was performed to identify important regulators and pathways involved in fruit ripening in a spontaneous late-ripening mutant ('fengwan' orange, citrus sinensis l. osbeck) and its wild type ('fengjie 72-1'). at the t ... | 2014 | 24600016 |
| differential transcriptional regulation of l-ascorbic acid content in peel and pulp of citrus fruits during development and maturation. | citrus fruits are an important source of ascorbic acid (asa) for human nutrition, but the main pathways involved in its biosynthesis and their regulation are still not fully characterized. to study the transcriptional regulation of asa accumulation, expression levels of 13 genes involved in asa biosynthesis, 5 in recycling and 5 in degradation were analyzed in peel and pulp of fruit of two varieties with different asa concentration: navel orange (citrus sinensis) and satsuma mandarin (citrus uns ... | 2014 | 24567029 |
| isolation, classification and transcription profiles of the ap2/erf transcription factor superfamily in citrus. | the ap2/erf gene family encodes plant-specific transcription factors. in model plants, ap2/erf genes have been shown to be expressed in response to developmental and environmental stimuli, and many function downstream of the ethylene, biotic, and abiotic stress signaling pathways. in citrus, ethylene is effective in regulation citrus fruit quality, such as degreening and aroma. however, information about the citrus ap2/erf family is limited, and would enhance our understanding of fruit responses ... | 2014 | 24566692 |
| characterization of mango (mangifera indica l.) transcriptome and chloroplast genome. | we characterized mango leaf transcriptome and chloroplast genome using next generation dna sequencing. the rna-seq output of mango transcriptome generated >12 million reads (total nucleotides sequenced >1 gb). de novo transcriptome assembly generated 30,509 unigenes with lengths in the range of 300 to ≥3,000 nt and 67× depth of coverage. blast searching against nonredundant nucleotide databases and several viridiplantae genomic datasets annotated 24,593 mango unigenes (80% of total) and identifi ... | 2014 | 24515595 |
| comparative analysis of surface wax in mature fruits between satsuma mandarin (citrus unshiu) and 'newhall' navel orange (citrus sinensis) from the perspective of crystal morphology, chemical composition and key gene expression. | surface wax of mature satsuma mandarin (citrus unshiu) and 'newhall' navel orange (citrus sinensis) was analysed by crystal morphology, chemical composition, and gene expression levels. the epicuticular and total waxes of both citrus cultivars were mostly composed of aldehydes, alkanes, fatty acids and primary alcohols. the epicuticular wax accounted for 80% of the total wax in the newhall fruits and was higher than that in the satsuma fruits. scanning electron microscopy showed that larger and ... | 2014 | 24491718 |
| characterization of the glutathione s-transferase gene family through ests and expression analyses within common and pigmented cultivars of citrus sinensis (l.) osbeck. | glutathione s-transferases (gsts) represent a ubiquitous gene family encoding detoxification enzymes able to recognize reactive electrophilic xenobiotic molecules as well as compounds of endogenous origin. anthocyanin pigments require gsts for their transport into the vacuole since their cytoplasmic retention is toxic to the cell. anthocyanin accumulation in citrus sinensis (l.) osbeck fruit flesh determines different phenotypes affecting the typical pigmentation of sicilian blood oranges. in th ... | 2014 | 24490620 |
| citrus sinensis annotation project (cap): a comprehensive database for sweet orange genome. | citrus is one of the most important and widely grown fruit crop with global production ranking firstly among all the fruit crops in the world. sweet orange accounts for more than half of the citrus production both in fresh fruit and processed juice. we have sequenced the draft genome of a double-haploid sweet orange (c. sinensis cv. valencia), and constructed the citrus sinensis annotation project (cap) to store and visualize the sequenced genomic and transcriptome data. cap provides gbrowse-bas ... | 2014 | 24489955 |
| determination of allergenic hydroperoxides in essential oils using gas chromatography with electron ionization mass spectrometry. | fragrance monoterpenes are widely used commercially due to their pleasant scent. in previous studies, we have shown that air-exposed monoterpenes form hydroperoxides that are strong skin sensitizers. methods for detection and quantification of the hydroperoxides in essential oils and scented products are thus desirable. due to thermolability and low uv absorbance, this is a complicated task. we have recently developed a sensitive lc-esi-ms method, but with limited structural information and sepa ... | 2014 | 24482420 |
| comparative de novo transcriptome analysis and metabolic pathway studies of citrus paradisi flavedo from naive stage to ripened stage. | grapefruit (citrus pardisi) is a popular citrus fruit that is a cross between a sweet orange and pummelo. this research article focuses on an in silico approach for comparative analysis of c. paradisi green flavedo (gf) and ethylene treated flavedo (etf) transcriptome data. our pathway analysis provides comprehensive information of genes playing significant role in different stages of ripening in fruit. de novo assembly was carried out using six different assemblers namely gs assembler, seqman n ... | 2014 | 24477585 |
| population of endogenous pararetrovirus genomes in carrizo citrange. | the complete genome sequences of three related endogenous pararetroviruses (eprvs) were obtained by 454 sequencing of nucleic acid extracts from carrizo citrange, used as a citrus rootstock. numerous homologous sequences have been found in the sweet orange genome. the new eprvs are most closely related to petunia vein-clearing virus. | 2014 | 24435856 |
| effect of sweet orange fruit waste diets and acidifier on haematology and serum chemistry of weanling rabbits. | a total of thirty-five mixed breed (35) rabbits of average weight of 700 g aged 5-6 weeks were allocated to seven treatments in a completely randomised design to investigate the effect of sweet orange fruit waste (sofw) and acidomix acidifier on haematology and serum chemistry. the diets were 0% sofw, 10% sofw with 0.5% acidomix, 10% sofw with 0.7 acidomix, 15% sofw with 0.5% acidifier, 15% sofw with 0.7% acidifier, 20% sofw with 0.5% acidifier, and 20% sofw with 0.7% acidifier. blood samples we ... | 2014 | 26464931 |
| citrange fruit extracts alleviate obesity-associated metabolic disorder in high-fat diet-induced obese c57bl/6 mouse. | obesity is becoming one of the global epidemics of the 21st century. in this study, the effects of citrange (citrus sinensis × poncirus trifoliata) fruit extracts in high-fat (hf) diet-induced obesity mice were studied. female c57bl/6 mice were fed respectively a chow diet (control), an hf diet, hf diet supplemented with 1% w/w citrange peel extract (cpe) or 1% w/w citrange flesh and seed extract (cfse) for 8 weeks. our results showed that both cpe and cfse regulated the glucose metabolic disord ... | 2013 | 24317433 |
| first evidence of c- and o-glycosyl flavone in blood orange (citrus sinensis (l.) osbeck) juice and their influence on antioxidant properties. | rp-lc-dad-esi-ms-ms separation/identification protocol has been employed for the identification and characterisation of nine c- and o-glycosyl flavonoids in moro (citrus sinensis (l.) osbeck) juice grown in southern italy. for the first time we reported the presence of five c-glycosyl flavones (lucenin-2, vicenin-2, stellarin-2, lucenin-2 4'-methyl ether and scoparin), a 3-hydroxy-3-methylglutaryl glycosyl flavonol (3-hydroxy-3-methylglutaryl glycosyl quercetin) and a flavone o-glycosides (chrys ... | 2014 | 24295703 |
| comprehensive insights on how 2,4-dichlorophenoxyacetic acid retards senescence in post-harvest citrus fruits using transcriptomic and proteomic approaches. | auxin-like 2,4-dichlorophenoxyacetic acid (2,4-d), a high-efficiency anti-stalling agent for the post-harvest fresh fruit industry, has had its use restricted due to environmental concerns. however, no other substitutes for 2,4-d are available to the post-harvest industry. insights into the molecular mechanism underlying the effects of 2,4-d on fruit quality preservation will provide a theoretical basis for exploring new safe and effective anti-stalling agents. this study comprehensively analyse ... | 2014 | 24215076 |
| mining of haplotype-based expressed sequence tag single nucleotide polymorphisms in citrus. | single nucleotide polymorphisms (snps), the most abundant variations in a genome, have been widely used in various studies. detection and characterization of citrus haplotype-based expressed sequence tag (est) snps will greatly facilitate further utilization of these gene-based resources. | 2013 | 24175923 |
| coumarin and furanocoumarin quantitation in citrus peel via ultraperformance liquid chromatography coupled with mass spectrometry (uplc-ms). | coumarins and furanocoumarins are secondary metabolites commonly found in citrus plants. these molecules are allelochemical compounds in plants that have controversial effects on humans, such as phototoxicity and the commonly described interactions with drugs, referred to as the "grapefruit juice effect". thus, it is important to develop a reliable method to identify and quantitate the coumarins and furanocoumarins in citrus extracts. for this purpose, we herein describe an ultraperformance liqu ... | 2013 | 24117278 |
| leaf cdna-aflp analysis of two citrus species differing in manganese tolerance in response to long-term manganese-toxicity. | very little is known about manganese (mn)-toxicity-responsive genes in citrus plants. seedlings of 'xuegan' (citrus sinensis) and 'sour pummelo' (citrus grandis) were irrigated for 17 weeks with nutrient solution containing 2 μm (control) or 600 μm (mn-toxicity) mnso₄. the objectives of this study were to understand the mechanisms of citrus mn-tolerance and to identify differentially expressed genes, which might be involved in mn-tolerance. | 2013 | 24034812 |
| metabolic engineering of β-carotene in orange fruit increases its in vivo antioxidant properties. | orange is a major crop and an important source of health-promoting bioactive compounds. increasing the levels of specific antioxidants in orange fruit through metabolic engineering could strengthen the fruit's health benefits. in this work, we have afforded enhancing the β-carotene content of orange fruit through blocking by rna interference the expression of an endogenous β-carotene hydroxylase gene (csβ-chx) that is involved in the conversion of β-carotene into xanthophylls. additionally, we h ... | 2014 | 24034339 |
| [spider diversity in cultures of citrus sinensis (rutaceae) in corrientes province, argentina]. | spiders are predators that reduce insect pest populations in agroecosystems. in spite that the presence of spider assemblages has been described in different crop plants, no reports have been done for citrus species in argentina. we studied the spider community associated with cultures of citrus sinensis in the province of corrientes, argentina, in two plots (am1 irrigated and am2 unirrigated) of 0.82 hectares each. spiders were collected monthly by using pitfall traps, hand capture, agitation o ... | 2013 | 24027921 |
| citrus nobiletin suppresses inducible nitric oxide synthase gene expression in interleukin-1β-treated hepatocytes. | nobiletin is a polymethoxylated flavone that is abundant in the peels of citrus fruits, such as citrus unshiu (satsuma mandarin) and citrus sinensis. the dried peels of c. unshiu (chinpi) have been included in several formulae of japanese kampo medicines. nobiletin may suppress the induction of inducible nitric oxide synthase (inos), which synthesizes the inflammatory mediator nitric oxide (no) in hepatocytes. | 2013 | 23958298 |
| species composition and abundance of brevipalpus spp. on different citrus species in mexican orchards. | we studied the abundance of brevipalpus spp. in citrus orchards in the mexican states of yucatan, quintana roo and campeche. mites were collected from 100 trees containing a mixture of citrus species where sweet orange was always the main species. eight collections were made at each location from february 2010 to february 2011. mites from the genus brevipalpus were separated from other mites surveyed and their abundance and relationships with the different citrus species were quantified througho ... | 2013 | 23949863 |
| the off-line combination of high performance liquid chromatography and comprehensive two-dimensional gas chromatography-mass spectrometry: a powerful approach for highly detailed essential oil analysis. | the present contribution is focused on the off-line combination of high performance liquid chromatography (hplc) and comprehensive two-dimensional gas chromatography-quadrupole mass spectrometry (gc×gc-quadms), and its application to the detailed qualitative analysis of essential oils. specifically, a silica column was exploited for the separation of the essential oil constituents in two groups, namely hydrocarbon and oxygenated compounds. after, each hplc-fraction was reduced in volume, and the ... | 2013 | 23890548 |
| the role of 1-deoxy-d-xylulose-5-phosphate synthase and phytoene synthase gene family in citrus carotenoid accumulation. | three 1-deoxy-d-xylulose-5-phosphate synthases (dxs) and three phytoene synthases (psy) were identified in citrus, from affymetrix genechip citrus genome array, genbank and public orange genome databases. tissue-specific expression analysis of these genes was carried out on fruit peel and flesh, flower and leaf of satsuma mandarin (citrus unshiu marc.) in order to determine their roles in carotenoid accumulation in different tissues. expression of citdxs1 and citpsy1 was highest in all test tiss ... | 2013 | 23883976 |
| a direct sensitivity comparison between flow-modulated comprehensive 2d and 1d gc in untargeted and targeted ms-based experiments. | the present contribution is focused on the measurement of the analytical sensitivity attained in untargeted/targeted ms/ms experiments, performed using flow-modulator comprehensive 2d and 1d gc. the comprehensive 2d experiment was performed by diverting part of the high flow (circa 80%) to flush the accumulation loop (about 28 ml/min) to waste, to reduce the gas flow entering the ion source. 1d analyses were performed through: (i) unmodulated and (ii) single column applications. an equivalent te ... | 2013 | 23868497 |
| interplay between aba and phospholipases a(2) and d in the response of citrus fruit to postharvest dehydration. | the interplay between abscisic acid (aba) and phospholipases a2 and d (pla2 and pld) in the response of citrus fruit to water stress was investigated during postharvest by using an aba-deficient mutant from 'navelate' orange named 'pinalate'. fruit from both varieties harvested at two different maturation stages (mature-green and full-mature) were subjected to prolonged water loss inducing stem-end rind breakdown (serb) in full-mature fruit. treatment with pla2 inhibitor aristolochic acid (at) a ... | 2013 | 23800664 |
| phosphoproteomic analysis of chromoplasts from sweet orange during fruit ripening. | like other types of plastids, chromoplasts have essential biosynthetic and metabolic activities which may be regulated via post-translational modifications, such as phosphorylation, of their resident proteins. we here report a proteome-wide mapping of in vivo phosphorylation sites in chromoplast-enriched samples prepared from sweet orange [citrus sinensis (l.) osbeck] at different ripening stages by titanium dioxide-based affinity chromatography for phosphoprotein enrichment with lc-ms/ms. a tot ... | 2014 | 23786612 |
| digital gene expression analysis of corky split vein caused by boron deficiency in 'newhall' navel orange (citrus sinensis osbeck) for selecting differentially expressed genes related to vascular hypertrophy. | corky split vein caused by boron (b) deficiency in 'newhall' navel orange was studied in the present research. the boron-deficient citrus exhibited a symptom of corky split vein in mature leaves. morphologic and anatomical surveys at four representative phases of corky split veins showed that the symptom was the result of vascular hypertrophy. digital gene expression (dge) analysis was performed based on the illumina hiseq™ 2000 platform, which was applied to analyze the gene expression profilin ... | 2013 | 23755275 |
| genome-wide characterization and expression analysis of genetic variants in sweet orange. | heterozyosity is an important feature of many plant genomes, and is related to heterosis. sweet orange, a highly heterozygous species, is thought to have originated from an inter-species hybrid between pummelo and mandarin. to investigate the heterozygosity of the sweet orange genome and examine how this heterozygosity affects gene expression, we characterized the genome of valencia orange for single nucleotide variations (snvs), small insertions and deletions (indels) and structural variations ... | 2013 | 23738603 |
| red orange: experimental models and epidemiological evidence of its benefits on human health. | in recent years, there has been increasing public interest in plant antioxidants, thanks to the potential anticarcinogenic and cardioprotective actions mediated by their biochemical properties. the red (or blood) orange (citrus sinensis (l.) osbeck) is a pigmented sweet orange variety typical of eastern sicily (southern italy), california, and spain. in this paper, we discuss the main health-related properties of the red orange that include anticancer, anti-inflammatory, and cardiovascular prote ... | 2013 | 23738032 |
| the in vitro antimicrobial activity of lavandula angustifolia essential oil in combination with other aroma-therapeutic oils. | the antimicrobial activity of lavandula angustifolia essential oil was assessed in combination with 45 other oils to establish possible interactive properties. the composition of the selected essential oils was confirmed using gc-ms with a flame ionization detector. the microdilution minimum inhibitory concentration (mic) assay was undertaken, whereby the fractional inhibitory concentration (σfic) was calculated for the oil combinations. when lavender oil was assayed in 1 : 1 ratios with other o ... | 2013 | 23737850 |
| effects of citrus sinensis juice on blood pressure. | citrus sinensis juice (csj) is a rich source of dietary flavonoids which reduce the risk of adverse cardiovascular events. this study aimed to examine the effects of four-week intake of natural and commercial orange (citrus sinensis) juice on blood pressure in healthy volunteers. | 2013 | 23696766 |
| itraq protein profile analysis of citrus sinensis roots in response to long-term boron-deficiency. | seedlings of citrus sinensis were fertilized with boron (b)-deficient (0μm h3bo3) or -sufficient (10μm h3bo3) nutrient solution for 15weeks. thereafter, itraq analysis was employed to compare the abundances of proteins from b-deficient and -sufficient roots. in b-deficient roots, 164 up-regulated and 225 down-regulated proteins were identified. these proteins were grouped into the following functional categories: protein metabolism, nucleic acid metabolism, stress responses, carbohydrate and ene ... | 2013 | 23628855 |
| tropospheric ozone reduces carbon assimilation in trees: estimates from analysis of continuous flux measurements. | high ground-level ozone concentrations are typical of mediterranean climates. plant exposure to this oxidant is known to reduce carbon assimilation. ozone damage has been traditionally measured through manipulative experiments that do not consider long-term exposure and propagate large uncertainty by up-scaling leaf-level observations to ecosystem-level interpretations. we analyzed long-term continuous measurements (>9 site-years at 30 min resolution) of environmental and eco-physiological param ... | 2013 | 23589473 |
| fumigant toxicity of citrus sinensis essential oil on musca domestica l. adults in the absence and presence of a p450 inhibitor. | essential oils (eos) are potential tools for controlling musca domestica l. in a fumigant assay, m. domestica adults treated with citrus sinensis eo (lc50=3.9mg/dm(3)), with (4r)(+)-limonene (95.1%) being its main component, died within 15min or less. the terpenes absorbed by the flies and their metabolites, analyzed using spme fiber, were (4r)(+)-limonene (lc50=6.2mg/dm(3)), α-pinene (lc50=11.5mg/dm(3)), β-pinene (lc50=6.4mg/dm(3)), and two new components, carveol (lc50=1122mg/dm(3)) and carvon ... | 2013 | 23545130 |
| metabolic responses to iron deficiency in roots of carrizo citrange [citrus sinensis (l.) osbeck. x poncirus trifoliata (l.) raf]. | the effects of iron (fe) deficiency on the low-molecular-weight organic acid (lmwoa) metabolism have been investigated in carrizo citrange (cc) [citrus sinensis (l.) osb. × poncirus trifoliata (l.) raf.] roots. major lmwoas found in roots, xylem sap and root exudates were citrate and malate and their concentrations increased with fe deficiency. the activities of several enzymes involved in the lmwoa metabolism were also assessed in roots. in the cytosolic fraction, the activities of malate dehyd ... | 2013 | 23462311 |
| modification of carotenoid levels by abscission agents and expression of carotenoid biosynthetic genes in 'valencia' sweet orange. | the effect of 5-chloro-3-methyl-4-nitro-1h-pyrazole (cmnp) and ethephon on peel color, flavedo carotenoid gene expression, and carotenoid accumulation was investigated in mature 'valencia' orange ( citrus sinensis l. osbeck) fruit flavedo at three maturation stages. abscission agent application altered peel color. cmnp was more effective than ethephon in promoting green-to-red (a) and blue-to-yellow (b) color at the middle and late maturation stages and total carotenoid changes at all maturation ... | 2013 | 23451824 |
| new excised-leaf assay method to test inoculativity of asian citrus psyllid (hemiptera: psyllidae) with candidatus liberibacter asiaticus associated with citrus huanglongbing disease. | the asian citrus psyllid, diaphorina citri kuwayama (hemiptera: psyllidae), is the primary vector of candidatus liberibacter asiaticus (las) associated with huanglongbing, or citrus greening, the most devastating citrus (citrus spp.) disease worldwide. here, we developed a new "excised-leaf assay" that can speed up las-inoculativity tests on asian citrus psyllid from the current 3-12 mo (when using whole citrus seedlings for inoculation) to only 2-3 wk. young adults of asian citrus psyllid that ... | 2013 | 23448011 |
| quantification of hesperidin in citrus-based foods using a fungal diglycosidase. | a simple enzymatic-spectrophotometric method for hesperidin quantification was developed by means of a specific fungal enzyme. the method utilises the diglycosidase α-rhamnosyl-β-glucosidase (ec 3.2.1.168) to quantitatively hydrolyse hesperidin to hesperetin, and the last is measured by its intrinsic absorbance in the uv range at 323 nm. the application of this method to quantify hesperidin in orange (citrus sinensis) juices was shown to be reliable in comparison with the standard method for fla ... | 2012 | 23442693 |
| a sensitive method for determination of allergenic fragrance terpene hydroperoxides using liquid chromatography coupled with tandem mass spectrometry. | different compositions of monoterpenes are utilized for their pleasant scent in cosmetics and perfumes. however, the most commonly used fragrance terpenes easily oxidize upon contact with air, forming strongly skin-sensitizing hydroperoxides. due to their thermolability and low uv absorbance, detection methods for hydroperoxides are scarce. for the first time, a simple and sensitive method using lc/esi-ms/ms was developed to quantitatively determine hydroperoxides from the common fragrance compo ... | 2013 | 23404959 |
| [identification of different citrus sinensis (l.) osbeck trees varieties using fourier transform infrared spectroscopy and hierarchical cluster analysis]. | researched on diversity of the spring leaf samples of seven different citrus sinensis (l.) osbeck varieties by fourier transform infrared (ftir) spectroscopy technology, the results showed that the fourier transform infrared spectra of seven varieties leaves was composited by the absorption band of cellulose and polysaccharide mainly, the wave number of characteristics absorption peaks were similar at their ftir spectra. however, there were some differences in shape of peaks and relatively absor ... | 2012 | 23387167 |
| metabolism in orange fruits is driven by photooxidative stress in the leaves. | in plants, stress signals propagate to trigger distant responses and thus stress acclimation in non-exposed organs. we tested here the hypothesis that leaves submitted to photooxidative stress may influence the metabolism of nearby fruits and thus quality criteria. leaves of orange trees (citrus sinensis (l.) osbeck cv. 'navelate') were acclimated to shade for 1 week and then submitted to full (fl) and medium light (ml) conditions. as expected, photoinhibition was detected in leaves of both fl a ... | 2013 | 23330573 |
| phytochemical profile and antioxidant activity of physiological drop of citrus fruits. | the phytochemical content and the antioxidant activity (aa) of physiological drop of the main citrus species grown in china were investigated. among the flavonoids, hesperidin was found mostly in mandarin and sweet orange, naringin was found mostly in sour orange, pummelo, grapefruit and a hybrid (gaocheng), narirutin was found in most varieties, neohesperidin was found in gaocheng and huyou, and nobiletin and tangeretin were found in most varieties. hydroxycinnamic acids were the main phenolic ... | 2013 | 23301602 |
| [effectiveness of antimicrobial formulations for acne based on orange (citrus sinensis) and sweet basil (ocimum basilicum l) essential oils]. | currently, the antimicrobial resistance has developed in bacterial strains involved in the development of acne. therefore, alternatives to antibiotic treatment have become necessary. | 2016 | 23235794 |
| the draft genome of sweet orange (citrus sinensis). | oranges are an important nutritional source for human health and have immense economic value. here we present a comprehensive analysis of the draft genome of sweet orange (citrus sinensis). the assembled sequence covers 87.3% of the estimated orange genome, which is relatively compact, as 20% is composed of repetitive elements. we predicted 29,445 protein-coding genes, half of which are in the heterozygous state. with additional sequencing of two more citrus species and comparative analyses of s ... | 2013 | 23179022 |
| a reference genetic map of c. clementina hort. ex tan.; citrus evolution inferences from comparative mapping. | most modern citrus cultivars have an interspecific origin. as a foundational step towards deciphering the interspecific genome structures, a reference whole genome sequence was produced by the international citrus genome consortium from a haploid derived from clementine mandarin. the availability of a saturated genetic map of clementine was identified as an essential prerequisite to assist the whole genome sequence assembly. clementine is believed to be a 'mediterranean' mandarin × sweet orange ... | 2012 | 23126659 |
| citrus phenylpropanoids and defence against pathogens. part ii: gene expression and metabolite accumulation in the response of fruits to penicillium digitatum infection. | the effect of infection of citrus sinensis (var. navelina) fruits with penicillium digitatum was studied at gene expression and metabolite levels. in this study, expression of genes involved in the phenylpropanoid pathway was studied in the flavedo (outer coloured part of the peel) and albedo (inner white part) in response to pathogen infection. results of the time-course experiment showed that maximal expression of 10 out of 17 phenylpropanoid genes analysed occurred at 48h post-inoculation, wh ... | 2013 | 23017425 |
| ceramides as possible nutraceutical compounds: characterization of the ceramides of the moro blood orange ( citrus sinensis ). | ceramides are presented as nutraceutical compounds for protection of colon carcinoma and as important cosmetic preparation components, increasing absorption through the skin. therefore, the ceramide (cer) content of moro blood oranges was determined by mass spectrometry. a total of 114 cer species were identified: ∼160 mg in the peels and ∼140 mg in the pulp per kilogram of oranges, expressed as "milligram equivalents of d18:1,17:0 cer". the predominant ceramides contained 4-hydroxy-8-sphingenin ... | 2012 | 22985176 |
| protective effects of sweet orange (citrus sinensis) peel and their bioactive compounds on oxidative stress. | protective effects of sweet orange (citrus sinensis) peel and their bioactive compounds on oxidative stress were investigated. according to hplc-dad and hplc-ms/ms analysis, hesperidin (hd), hesperetin (ht), nobiletin (nt), and tangeretin (tt) were present in water extracts of sweet orange peel (wesp). the cytotoxic effect in 0.2mm t-bhp-induced hepg2 cells was inhibited by wesp and their bioactive compounds. the protective effect of wesp and their bioactive compounds in 0.2mm t-bhp-induced hepg ... | 2012 | 22980779 |
| on line characterization of 58 phenolic compounds in citrus fruit juices from spanish cultivars by high-performance liquid chromatography with photodiode-array detection coupled to electrospray ionization triple quadrupole mass spectrometry. | polyphenol profile of citrus juices of sweet orange, tangerine, lemon and grapefruit from spanish cultivars was obtained by high-performance liquid chromatography with diode array detection coupled to electrospray ionization and triple quadrupole mass spectrometry. fifty eight phenolic compounds of five different classes were identified in these citrus juices. flavanone: o-dihexoside of naringenin; flavones: apigenin-7-o-rutinoside-4'-o-glucoside, luteolin-7-o-neohesperidoside-4'-o-glucoside, lu ... | 2012 | 22967544 |
| d-limonene rich volatile oil from blood oranges inhibits angiogenesis, metastasis and cell death in human colon cancer cells. | to identify the chemical constituents of volatile oil from blood orange (citrus sinensis (l) osbeck) and understand the possible mechanisms of inhibition of colon cancer cell proliferation. | 2012 | 22935404 |
| analysis of full-length sequences of two citrus yellow mosaic badnavirus isolates infecting citrus jambhiri (rough lemon) and citrus sinensis l. osbeck (sweet orange) from a nursery in india. | citrus yellow mosaic badna virus (cmbv), a member of the family caulimoviridae, genus badnavirus is the causative agent of mosaic disease among citrus species in southern india. despite its reported prevalence in several citrus species, complete information on clear functional genomics or functional information of full-length genomes from all the cmbv isolates infecting citrus species are not available in publicly accessible databases. cmbv isolates from rough lemon and sweet orange collected fr ... | 2012 | 22926812 |
| a new strategy employed for identification of sweet orange cultivars with rapd markers. | we optimized rapd techniques by increasing the length of rapd primers and performing a strict screening of pcr annealing temperature to distinguish 60 sweet orange cultivars from the research institute of pomology at the chinese academy of agricultural sciences. a new approach called cultivar identification diagram (cid) was used to improve the efficiency of rapd markers for cultivar identification. thirteen effective primers were first screened from 54 rapd arbitrary 11-mer primers based on the ... | 2012 | 22911591 |
| a comparison of the ultrastructure and composition of fruits' cuticular wax from the wild-type 'newhall' navel orange (citrus sinensis [l.] osbeck cv. newhall) and its glossy mutant. | the altered ultrastructure and composition of cuticular wax from 'glossy newhall' (mt) fruits lead to its glossy phenotype. a novel mutant derived from the wild-type (wt) 'newhall' navel orange (citrus sinensis [l.] osbeck cv. newhall), named 'glossy newhall' (mt), which produced much more glossy fruits that were easily distinguishable from the wt fruits was characterized in this report. the total wax loads of both wt and mt fruits varied considerably during the fruit development. the most abund ... | 2012 | 22892682 |
| the citrus aba signalosome: identification and transcriptional regulation during sweet orange fruit ripening and leaf dehydration. | the abscisic acid (aba) signalling core in plants include the cytosolic aba receptors (pyr/pyl/rcars), the clade-a type 2c protein phosphatases (pp2cas), and the subclass iii snf1-related protein kinases 2 (snrk2s). the aim of this work was to identify these aba perception system components in sweet orange and to determine the influence of endogenous aba on their transcriptional regulation during fruit development and ripening, taking advantage of the comparative analysis between a wild-type and ... | 2012 | 22888124 |
| potential of moringa oleifera root and citrus sinensis fruit rind extracts in the treatment of ulcerative colitis in mice. | the plant moringa oleifera lam (moringaceae), commonly known as the drumstick tree, is an indigenous species in india. this species has been of interest to researchers because traditionally its roots are reported in the treatment of ulcerative colitis (uc). traditionally it is reported that citrus sinensis linn (rutaceae) fruit rind when combined with m. oleifera will increase the efficacy of the plant in the treatment of uc. | 2012 | 22849565 |
| effect of sweet orange aroma on experimental anxiety in humans. | the objective of this study was to evaluate the potential anxiolytic effect of sweet orange (citrus sinensis) aroma in healthy volunteers submitted to an anxiogenic situation. | 2012 | 22849536 |
| comparative transcriptional and anatomical analyses of tolerant rough lemon and susceptible sweet orange in response to 'candidatus liberibacter asiaticus' infection. | although there are no known sources of genetic resistance, some citrus spp. are reportedly tolerant to huanglongbing (hlb), presumably caused by 'candidatus liberibacter asiaticus'. time-course transcriptional analysis of tolerant rough lemon (citrus jambhiri) and susceptible sweet orange (c. sinensis) in response to 'ca. l. asiaticus' infection showed more genes differentially expressed in hlb-affected rough lemon than sweet orange at early stages but substantially fewer at late time points, po ... | 2012 | 22809274 |
| the influence of citrus rootstocks on the relationship between the mite brevipalpus phoenicis and citrus leprosis disease. | leprosis is one of the most serious citrus plant diseases. leprosis-affected plants, especially sweet orange [citrus sinensis (l.) osbeck], which is the most widely cultivated citrus fruit worldwide, show reduced photosynthetic capacity and severe defoliation. the aim was to evaluate the relationship between the brevipalpus phoenicis (geijskes) vector mite and citrus leprosis disease in pera sweet orange plants grafted on different rootstocks. data were analysed using numerical classification an ... | 2013 | 22807324 |
| field performance of transgenic citrus trees: assessment of the long-term expression of uida and nptii transgenes and its impact on relevant agronomic and phenotypic characteristics. | the future of genetic transformation as a tool for the improvement of fruit trees depends on the development of proper systems for the assessment of unintended effects in field-grown gm lines. in this study, we used eight transgenic lines of two different citrus types (sweet orange and citrange) transformed with the marker genes β-glucuronidase (uida) and neomycin phosphotransferase ii (nptii) as model systems to study for the first time in citrus the long-term stability of transgene expression ... | 2012 | 22794278 |
| transcriptional profiling of genes involved in embryogenic, non-embryogenic calluses and somatic embryogenesis of valencia sweet orange by ssh-based microarray. | somatic embryogenesis (se) is a most promising technology that is used for in vitro germplasm conservation and genetic improvement via biotechnological approaches in citrus. herein, three suppression subtractive hybridization (ssh) libraries were constructed using calluses of citrus sinensis cv. 'valencia' to explore the molecular mechanisms that underlie the se in citrus. a total of 880 unisequences were identified by microarray screening based on these three ssh libraries. gene ontology analys ... | 2012 | 22622359 |
| identification of differentially expressed genes in a spontaneous altered leaf shape mutant of the navel orange [citrus sinensis (l.) osbeck]. | most of the economically important citrus cultivars have originated from bud mutations. leaf shape and structure are important factors that impact plant photosynthesis. we found a spontaneous bud mutant exhibiting a narrow leaf phenotype in navel orange [citrus sinensis (l.) osbeck]. to identify and characterize the genes involved in the formation of this trait, we performed suppression subtractive hybridization (ssh) and macroarray analysis. a total of 221 non-redundant differentially expressed ... | 2012 | 22609459 |