Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| temperature thresholds and degree-day model for marmara gulosa (lepidoptera: gracillariidae). | the developmental thresholds for marmara gulosa guillén & davis (lepidoptera: gracillariidae) were investigated in the laboratory by using 17, 21, 25, 29, and 33 degrees c. the lowest mortality occurred in cohorts exposed to 25 and 29 degrees c. other temperatures caused >10% mortality primarily in egg and first and second instar sap-feeding larvae. linear regression analysis approximated the lower developmental threshold at 12.2 degrees c. high mortality and slow developmental rate at 33 degree ... | 2011 | 21882694 |
| physical location of the carotenoid biosynthesis genes psy and β-lcy in capsicum annuum (solanaceae) using heterologous probes from citrus sinensis (rutaceae). | carotenoids are responsible for a range of fruit colors in different hot pepper (capsicum) varieties, from white to deep red. color traits are genetically determined by three loci, y, c1, and c2, which are associated with carotenogenic genes. although such genes have been localized on genetic maps of capsicum and anchored in lycopersicon and solanum, physical mapping in capsicum has been restricted to only a few clusters of some multiple copy genes. heterologous probes from single copy genes hav ... | 2011 | 21425090 |
| to compare the efficacy of two kinds of zhizhu pills in the treatment of functional dyspepsia of spleen-deficiency and qi-stagnation syndrome: a randomized group sequential comparative trial. | in traditional chinese medicine (tcm) theory, functional dyspepsia (fd) can be divided into different syndromes according to different clinical symptoms and signs, and the most common one is spleen-deficiency and qi-stagnation syndrome that can be treated by chinese traditional patent medicine--two kinds of zhizhu pills, between which the primary difference in ingredients is that one contains immature orange fruit of citrus aurantium l.(ifca) and the other contains that of citrus sinensis osbeck ... | 2011 | 21762493 |
| activity of citrus essential oils against escherichia coli o157:h7 and salmonella spp. and effects on beef subprimal cuts under refrigeration. | escherichia coli o157:h7 and salmonella spp. are bacterial pathogens often associated with beef, and cause many cases of foodborne illness each year in the united states. during beef slaughter and processing, these bacteria may spread from the hide or intestines to the carcass. the objective of this research was to investigate the use of naturally occurring compounds citrus essential oils (ceos) extracted from orange peel to reduce or eliminate these pathogens at the chilling stage of processing ... | 2011 | 22417514 |
| cold treatment of ceratitis capitata (diptera: tephritidae) in oranges using a larval endpoint. | south africa currently exports fresh citrus (citrus spp.) fruit to japan using an in-transit cold treatment protocol of 14 d or 12 d at temperatures <0 degrees c for treatment of ceratitis capitata (wiedemann) (diptera: tephritidae) in 'clementine' mandarins (citrus reticulata blanco) and other citrus types, respectively. to reduce the risk of chilling injury with this treatment, research was conducted with temperatures >0 degrees c. earlier south african research had shown that young (6-d-old) ... | 2011 | 21882680 |
| mosquito larvicidal activity of citrus limonoids against aedes albopictus. | citrus limonoids, nomilin and limonin, were used for larvicidal assay against aedes albopictus utilizing who methodology. lc(50s) were 305.83, 176.08, and 136.07 μm for nomilin and 850.09, 600.72, and 407.09 μm for limonin after 24, 48, and 72 h, respectively. lt(50) assays exhibited that savage citrange oil was the best at all concentrations (400, 500, 600, and 700 ppm) while fairchild and cassa grande were the weakest oils at 400 ppm, but at 500, 600, and 700 ppm, carrizo citrange remained at ... | 2011 | 21212981 |
| biomimetic preparation of polymer-supported free radical scavenging, cytocompatible and antimicrobial "green" silver nanoparticles using aqueous extract of citrus sinensis peel. | in the pursuit of making the nanoscale-research greener, the utilization of the reductive potency of a common byproduct of food processing industry i.e. orange peel is reported here to prepare biopolymer-templated "green" silver nanoparticles. aqueous extract of orange peel at basic ph was exploited to prepare starch supported nanoparticles under ambient conditions. the compositional abundance of pectins, flavonoids, ascorbic acid, sugars, carotenoids and myriad other flavones may be envisaged f ... | 2011 | 21316933 |
| selection and validation of reference genes for gene expression studies by reverse transcription quantitative pcr in xanthomonas citri subsp. citri during infection of citrus sinensis. | xanthomonas citri subsp. citri (xcc) causes citrus canker, a worldwide disease found mainly in sweet oranges (citrus sinensis (l.) osbeck). the expression of nine candidate internal reference genes was analyzed in xcc grown alone and during c. sinensis infection to identify genes most suitable for comparative expression studies in xcc using reverse transcription quantitative pcr (qrt-pcr). the stability of these genes was determined using the programs genorm, normfinder and bestkeeper. the genes ... | 2011 | 21318633 |
| antioxidant capacity of hesperidin from citrus peel using electron spin resonance and cytotoxic activity against human carcinoma cell lines. | hesperidin is a flavonoid that has various pharmacological activities including anti-inflammatory, antimicrobial and antiviral activities. | 2011 | 21323480 |
| citrus tristeza virus infection induces the accumulation of viral small rnas (21-24-nt) mapping preferentially at the 3'-terminal region of the genomic rna and affects the host small rna profile. | to get an insight into the host rna silencing defense induced by citrus tristeza virus (ctv) and into the counter defensive reaction mediated by its three silencing suppressors (p25, p20 and p23), we have examined by deep sequencing (solexa-illumina) the small rnas (srnas) in three virus-host combinations. our data show that ctv srnas: (i) represent more than 50% of the total srnas in mexican lime and sweet orange (where ctv reaches relatively high titers), but only 3.5% in sour orange (where th ... | 2011 | 21327514 |
| ectopic expression of mdspds1 in sweet orange (citrus sinensis osbeck) reduces canker susceptibility: involvement of hôééoôéé production and transcriptional alteration. | enormous work has shown that polyamines are involved in a variety of physiological processes, but information is scarce on the potential of modifying disease response through genetic transformation of a polyamine biosynthetic gene. | 2011 | 21439092 |
| inhibition of tnf-α/ifn-γ induced rantes expression in hacat cell by naringin. | context: naringin is a bioflavonoid derivative and is predominantly found in citrus paradisi macf., citrus sinensis (linn.) osbeck, citrus unshiu marc., citrus reticulata blanco cv. nobilis, citrus tachibana (makino) tanaka, citrus junos sieb. ex tanaka (rutaceae), and related citrus species. it has anti-inflammatory effects that have been well-documented, but the mechanism is poorly characterized. objective: the effect of naringin on production of rantes (regulated upon activation normal t-cell ... | 2011 | 21500970 |
| host status of grapefruit and valencia oranges for anastrepha serpentina and anastrepha ludens (diptera: tephritidae). | anastrepha serpentina (wiedemann) (diptera: tephritidae) is sporadically captured in the rio grande valley of texas. although its preferred hosts are in the sapotaceae family, several varieties of citrus, including grapefruit and oranges are listed as alternate hosts. although mexican fruit fly, anastrepha ludens (loew), is known to be a major pest of citrus, doubt exists as to the status of citrus as a breeding host for a. serpentina. to evaluate the host status of commercial citrus for a. serp ... | 2011 | 21510184 |
| terpene down-regulation in orange reveals the role of fruit aromas in mediating interactions with insect herbivores and pathogens. | plants use volatile terpene compounds as odor cues for communicating with the environment. fleshy fruits are particularly rich in volatiles that deter herbivores and attract seed dispersal agents. we have investigated how terpenes in citrus fruit peels affect the interaction between the plant, insects, and microorganisms. because limonene represents up to 97% of the total volatiles in orange (citrus sinensis) fruit peel, we chose to down-regulate the expression of a limonene synthase gene in ora ... | 2011 | 21525333 |
| biosynthesis of silver nanoparticles using citrus sinensis peel extract and its antibacterial activity. | biosynthesis of silver nanoparticles (agnps) was achieved by a novel, simple green chemistry procedure using citrus sinensis peel extract as a reducing and a capping agent. the effect of temperature on the synthesis of silver nanoparticles was carried out at room temperature (25°c) and 60°c. the successful formation of silver nanoparticles has been confirmed by uv-vis, ftir, xrd, edax, fesem and tem analysis and their antibacterial activity against escherichia coli, pseudomonas aeruginosa (gram- ... | 2011 | 21536485 |
| tetraploidization events by chromosome doubling of nucellar cells are frequent in apomictic citrus and are dependent on genotype and environment. | background and aims polyploidy is a major component of plant evolution. the citrus gene pool is essentially diploid but tetraploid plants are frequently encountered in seedlings of diploid apomictic genotypes. the main objectives of the present study were to establish the origin of these tetraploid plants and to ascertain the importance of genotypic and environmental factors on tetraploid formation. methods tetraploid seedlings from 30 diploid apomictic genotypes were selected by flow cytometry ... | 2011 | 21586529 |
| the sucrose synthase-1 promoter from citrus sinensis directs expression of the β-glucuronidase reporter gene in phloem tissue and in response to wounding in transgenic plants. | interest in phloem-specific promoters for the engineering of transgenic plants has been increasing in recent years. in this study we isolated two similar, but distinct, alleles of the citrus sinensis sucrose synthase-1 promoter (cssus1p) and inserted them upstream of the β-glucuronidase (gus) gene to test their ability to drive expression in the phloem of transgenic arabidopsis thaliana and nicotiana tabacum. although both promoter variants were capable of conferring localized gus expression in ... | 2011 | 21594624 |
| controlling for sugar and ascorbic acid, a mixture of flavonoids matching navel oranges significantly increases human postprandial serum antioxidant capacity. | fruit and vegetable consumption reduces the risk for cardiovascular disease development. the postprandial state is an important contributor to chronic disease development. orange flavonoids may reduce postprandial oxidation. it was hypothesized that a mixture of orange flavonoids would reduce postprandial oxidation better than a single orange flavonoid or orange sugar and ascorbic acid, but not as well as orange juice, when consumed with a typical breakfast. a placebo-controlled crossover trial ... | 2011 | 21840468 |
| ectopic expression of the p23 silencing suppressor of citrus tristeza virus differentially modifies viral accumulation and tropism in two transgenic woody hosts. | citrus tristeza virus (ctv), a phloem-restricted closterovirus infecting citrus, encodes three different silencing suppressors (p25, p20 and p23), one of which (p23) is a pathogenicity determinant that induces aberrations resembling ctv symptoms when expressed ectopically in transgenic citrus hosts. in this article, the effect of p23 ectopic expression on virus infection was examined in sweet orange (swo), a highly susceptible host, and sour orange (so), which severely restricts ctv cell-to-cell ... | 2011 | 21726389 |
| colonization of citrus seed coats by 'candidatus liberibacter asiaticus': implications for seed transmission of the bacterium. | huanglongbing is an economically damaging disease of citrus associated with infection by 'ca. liberibacter asiaticus'. transmission of the organism via infection of seeds has not been demonstrated but is a concern since some citrus varieties, particularly those used as rootstocks in commercial plantings are propagated from seed. we compared the incidence of detection of 'ca. liberibacter asiaticus' dna in individual fruit peduncles, seed coats, seeds and in germinated seedlings from 'sanguenelli ... | 2011 | 21714779 |
| comparison of cell wall metabolism in the pulp of three cultivars of 'nanfeng' tangerine differing in mastication trait. | background: like sweet orange (citrus sinensis), tangerine (citrus reticulata) is another citrus crop grown widely throughout the world. however, whether it shares a common mechanism with sweet orange in forming a given mastication trait is still unclear. in this study, three 'nanfeng' tangerine cultivars, 'yangxiao-26' ('yx-26') with inferior mastication trait, elite 'yx-26' with moderate mastication trait and 'miguang' ('mg') with superior mastication trait, were selected to investigate the fo ... | 2011 | 21732384 |
| comparative itraq proteome and transcriptome analyses of sweet orange infected by candidatus liberibacter asiaticus. | citrus huanglongbing (hlb) has been threatening citrus production worldwide. in this study, a comparative proteomic approach was applied to understand the pathogenic process of hlb in affected sweet orange leaves. using the isobaric tags for relative and absolute quantification (itraq) technique, we identified 686 unique proteins in the mature leaves of both mock-inoculated and diseased 'madam vinous' sweet orange plants. of the identified proteins, 20 and 10 were differentially expressed in lea ... | 2011 | 21838733 |
| isolation, identification and sequence analysis of a thioredoxin h gene, a member of subgroup iii of h-type trxs from grape (vitis vinifera l. cv. askari). | thioredoxins (trxs) are small ubiquitous proteins which play a regulatory role in a variety of cellular processes. in contrast to other organisms, plants have a great number of trx types, consisting of six well-defined groups: f, m, x, and y in chloroplasts, o in mitochondria, and h mainly in cytosol. a full-length cdna, designated vvcxxs2, encoding trx h polypeptide was isolated and cloned from grape (vitis vinifera l. cv. askari) berries organ by reverse transcription polymerase chain reaction ... | 2011 | 21732058 |
| metal analysis in citrus sinensis fruit peel and psidium guajava leaf. | the determination of metal traces is very important because they are involved in biological cycles and indicate high toxicity. the objective of the present study is to measure the levels of heavy metals and mineral ions in medicinally important plant species, citrus sinensis and psidium guajava. this study investigates the accumulation of copper (cu), zinc (zn), cadmium (cd), aluminum (al), mercury (hg), arsenic (as), selenium (se) and inorganic minerals like calcium (ca) and magnesium (mg) in c ... | 2011 | 21976824 |
| Comparison of ascorbate metabolism in fruits of two citrus species with obvious difference in ascorbate content in pulp. | Citrus fruit is widely consumed and provides ascorbate for human health. The ascorbate content in pulp is generally higher in orange (Citrus sinensis Osb.) than in Satsuma mandarin (Citrus unshiu Marc.). However, what contributes to such difference is still unknown. In the present study, ascorbate accumulation, expression profiles of genes involved in L-galactose pathway and activity changes of enzymes related with L-ascorbic acid (AA) oxidation and recycling were investigated during fruit devel ... | 2011 | 21925761 |
| Regulation of carotenoid accumulation and the expression of carotenoid metabolic genes in citrus juice sacs in vitro. | In the present study, to investigate the mechanisms regulating carotenoid accumulation in citrus, a culture system was set up in vitro with juice sacs of three citrus varieties, Satsuma mandarin (Citrus unshiu Marc.), Valencia orange (Citrus sinensis Osbeck), and Lisbon lemon (Citrus limon Burm.f.). The juice sacs of all the three varieties enlarged gradually with carotenoid accumulation. The changing patterns of carotenoid content and the expression of carotenoid metabolic genes in juice sacs i ... | 2011 | 21994171 |
| evaluation of effects of zingiber officinale on salivation in rats. | there are some herbal plants in iranian traditional system of medicine which are believed to be excellent remedies to alleviate the symptoms of xerostomia. the aim of the present study was to evaluate the effect of systemic administration of seven different herbal extracts on the rate of salivation in rats. the extracts of 7 herbs; zingiber officinale roscoe (zingiberaceae), citrus sinensis (l.) osbeck (rutaceae), artemisia absinthium l. (asteraceae), cichorium intybus l. (asteraceae), pimpinell ... | 2011 | 21874635 |
| interaction of hesperetin glucuronide conjugates with human bcrp, mrp2 and mrp3 as detected in membrane vesicles of overexpressing baculovirus-infected sf9 cells. | the citrus flavonoid hesperetin (4'-methoxy-3',5,7-trihydroxyflavanone) is the aglycone of hesperidin, the major flavonoid present in sweet oranges. hesperetin 7-o-glucuronide (h7g) and hesperetin 3'-o-glucuronide (h3'g) are the two most abundant metabolites of hesperetin in vivo. in this study, their interaction with specific abc transporters, believed to play a role in the disposition and bioavailability of hesperetin, was studied using sf9 membranes from cells overexpressing human bcrp (abcg2 ... | 2011 | 22083890 |
| characterization and comparison of volatile constituents of juice and peel from clementine, mandarin and their hybrids. | the volatile compositions of juice and peel of clementine (citrus reticulata x citrus sinensis var. commune), mandarin (citrus reticulata blanco var. willow leaf) and their hybrids were analyzed by headspace solid-phase extraction (hs-spme) coupled with gas chromatography and either a flame ionization detector (fid) or a mass spectrometer (ms). the major compounds of the volatile fractions of clementine and mandarin were limonene and limonene/gamma-terpinene, respectively. the volatile compositi ... | 2011 | 22164792 |
| juice components and antioxidant capacity of four tunisian citrus varieties. | juices from four citrus species of tunisia were investigated mainly for quality parameters and antioxidant capacity. | 2011 | 20862741 |
| an attempt of postharvest orange fruit rot control using essential oils from mediterranean plants. | twelve essential oils from mediterranean aromatic plants were tested at different doses against four fungi known as causal agents of post-harvest orange fruit rot: botrytis cinerea, penicillium italicum, phytophthora citrophthora, and rhizopus stolonifer. essential oils were obtained from hyssopus officinalis, lavandula angustifolia, majorana hortensis, melissa officinalis, ocimum basilicum, origanum vulgare, salvia officinalis, and thymus vulgaris (family lamiaceae), verbena officinalis (family ... | 2010 | 20874226 |
| citrus fruit extracts reduce advanced glycation end products (ages)- and h(2)o(2)-induced oxidative stress in human adipocytes. | diabetes is a reactive oxygen species (ros)-mediated pathology, with a worldwide prevalence estimated to double by 2030. a major effort has been launched to find therapeutic means to improve health conditions of diabetic patients. recent data show that supplemental natural antioxidants represent a potential strategy as adjunct therapy. despite the major role of adipocytes in the etiology of diabetes, little is known about the effect of natural antioxidants on adipocyte response to oxidative stre ... | 2010 | 20882960 |
| effect of chlorine dioxide gas on salmonella enterica inoculated on navel orange surfaces and its impact on the quality attributes of treated oranges. | microorganisms, including pathogens of public health significance, have been shown to contaminate orange juice during the mechanical extraction of juice. the problem gets exacerbated when washed oranges have high initial microbial load, due to an insufficient postharvest treatment. the objective of this study was to investigate the reduction of salmonella enterica on orange surfaces using clo₂ gas treatments to achieve a 5 log reduction, consistent with the recommendations of the u.s. department ... | 2010 | 20932090 |
| effects of pymetrozine, an antifeedant of hemiptera, on asian citrus psyllid, diaphorina citri, feeding behavior, survival and transmission of candidatus liberibacter asiaticus. | vector-borne plant disease management can be enhanced by deployment of antifeedants in addition to the use of broad-spectrum neurotoxic insecticides. the effects of pymetrozine on asian citrus psyllid (acp), diaphorina citri kuwayama, feeding behaviour, survival and transmission of candidatus liberibacter asiaticus (las), the presumed causal pathogen of huanglongbing, were investigated. | 2010 | 20960465 |
| transformation of sweet orange [citrus sinensis (l.) osbeck] with ptha-nls for acquiring resistance to citrus canker disease. | the cooh terminal of ptha encoding three nuclear localizing signals (nls) was amplified by polymerase chain reaction (pcr) from the plasmid of xanthomonas axonopodis pv. citri, the pathogen of citrus canker disease. then the sense and antisense strands of the nls were cloned into pbi121 vector. ptha-nls driven by the camv35 s promoter was transferred into sweet orange via agrobacterium -mediated transformation. successful integration was confirmed by pcr and southern blotting, and 12 sense-nls ( ... | 2010 | 20972821 |
| effective sampling range of a synthetic protein-based attractant for ceratitis capitata (diptera: tephritidae). | studies were conducted in honduras to determine effective sampling range of a female-targeted protein-based synthetic attractant for the mediterranean fruit fly, ceratitis capitata (wiedemann) (diptera: tephritidae). multilure traps were baited with ammonium acetate, putrescine, and trimethylamine lures (three-component attractant) and sampled over eight consecutive weeks. field design consisted of 38 traps (over 0.5 ha) placed in a combination of standard and high-density grids to facilitate ge ... | 2010 | 21061993 |
| antiplasmodial activity of botanical extracts against plasmodium falciparum. | the absence of a vaccine and the rampant resistance to almost all antimalarial drugs have accentuated the urgent need for new antimalarial drugs and drug targets for both prophylaxis and chemotherapy. the aim of the study was to discover effective plant extracts against plasmodium falciparum. in the present study, the hexane, chloroform, ethyl acetate, acetone, and methanol extracts of citrus sinensis (peel), leucas aspera, ocimum sanctum, phyllanthus acidus (leaf), terminalia chebula (seed) wer ... | 2010 | 21079994 |
| resistance to citrus psorosis virus in transgenic sweet orange plants is triggered by coat protein-rna silencing. | the lack of naturally occurring resistance to citrus psorosis virus (cpsv) has demanded exploitation of a transgenic approach for the development of cpsv-resistant sweet orange plants. transgenic sweet orange plants producing intron-hairpin rna transcripts (ihprna) corresponding to viral cp, 54k or 24k genes were generated and analyzed at the molecular and phenotypic levels. two independent cpsv challenge assays demonstrated that expression of ihprna derived from the cp gene (ihpcp) provided a h ... | 2010 | 21084056 |
| rapid simultaneous determination of amines and organic acids in citrus using high-performance liquid chromatography. | rapid analytical method for the simultaneous separation and determination of amines and organic acids is a vital interest for quality control of citrus and their products. in the present study, a simultaneous high performance liquid chromatography (hplc) method for the rapid separation of three amines and two organic acids was developed. chromatographic separation of compounds was achieved using xbridge c(18) column at ambient temperature, with an isocratic mobile phase of 3mm phosphoric acid at ... | 2010 | 21147342 |
| elsinoë fawcettii and elsinoë australis: the fungal pathogens causing citrus scab. | elsinoë fawcettii and e. australis are important pathogens of citrus. both species are known to produce red or orange pigments, called elsinochrome. elsinochrome is a nonhost-selective phytotoxin and is required for full fungal virulence and lesion formation. this article discusses the taxonomy, epidemiology, genetics and pathology of the pathogens. it also provides a perspective on the cellular toxicity, biosynthetic regulation and pathological role of elsinochrome phytotoxin. taxonomy: elsinoë ... | 2010 | 21199563 |
| microbial inhibitory and radical scavenging activities of cold-pressed terpeneless valencia orange (citrus sinensis) oil in different dispersing agents. | due to their low solubility in water, oil-based bioactive compounds require dispersion in a surface-active agent or appropriate solvents to ensure maximum contact with microorganisms. these combinations, however, may change their physical and/or chemical characteristics and consequently alter the desired functionality. the objective of this study was to determine the impact of selected dispersing agents, ethanol, dimethyl sulfoxide (dmso), and tween-80, on cold-pressed terpeneless (cpt) valencia ... | 2010 | 20355124 |
| chemical profile, antifungal, antiaflatoxigenic and antioxidant activity of citrus maxima burm. and citrus sinensis (l.) osbeck essential oils and their cyclic monoterpene, dl-limonene. | the study deals with antifungal, antiaflatoxigenic and antioxidant activity of citrus maxima and citrus sinensis essential oils (eos) and their phytochemical composition. the eos were obtained by hydrodistillation and their chemical profile was determined through gc and gc-ms analysis. both the eos and their 1:1 combination showed broad fungitoxic spectrum against different food contaminating moulds. the eos and their combination completely inhibited aflatoxin b(1) (afb(1)) production at 500 ppm ... | 2010 | 20385194 |
| the effects of host, geographic origin, and gender on the thermal requirements of diaphorina citri (hemiptera: psyllidae). | diaphorina citri kuwayama (hemiptera: psyllidae) is the vector of the bacteria that causes citrus greening and is considered one of the world's most important citrus diseases. we examined how host, geographic region, and gender affect the thermal requirements of d. citri. the insects were reared in climatic chambers at constant temperatures of 18, 20, 22, 25, 28, 30, and 32 +/- 1 degrees c, 70 +/- 10% rh, and a 14 h photophase. host plants for d. citri included orange (citrus sinensis [rutaceae] ... | 2010 | 20388302 |
| acaricidal activity against tetranychus urticae and chemical composition of peel essential oils of three citrus species cultivated in ne brazil. | the repellency and fumigant toxicities of the peel essential oils of citrus sinensis var. pêra (lp), c. sinensis var. mimo (lm), and c. aurantium (ll) cultivated in northeast brazil were evaluated against tetranychus urticae. analysis of the oils by gc and gc/ms led to the identification of twenty-eight components, which represented 99.9%, 99.7% and 99.3% of the total constituents of the lp, lm and ll oils, respectively. limonene was the main component found in all three oils. other main compone ... | 2010 | 20420330 |
| stereoselective conjugation, transport and bioactivity of s- and r-hesperetin enantiomers in vitro. | the flavanone hesperetin ((+/-)-4'-methoxy-3',5,7-trihydroxyflavanone) is the aglycone of hesperidin, which is the major flavonoid present in sweet oranges. hesperetin contains a chiral c-atom and so can exist as an s- and r-enantiomer, however, in nature 2s-hesperidin and its s-hesperetin aglycone are predominant. the present study reports a chiral hplc method to separate s- and r-hesperetin on an analytical and semipreparative scale. this allowed characterization of the stereoselective differe ... | 2010 | 20441166 |
| chemical characterization of orange juice from trees infected with citrus greening (huanglongbing). | the effects due to candidatus liberibacter infection, commonly called citrus greening or huanglongbing (hlb), on volatile and nonvolatile components of orange juices, oj, were examined using gc-ms and high-performance liquid chromatography (hplc). hlb symptomatic, asymptomatic, and control "hamlin" and "valencia" oranges were harvested from december to may during the 2007 to 2008 harvest season. brix/acid levels in control and asymptomatic juices were similar but symptomatic juices were as much ... | 2010 | 20492226 |
| glutathione peroxidase regulation of reactive oxygen species level is crucial for in vitro plant differentiation. | phospholipid hydroperoxide glutathione peroxidase (phgpx) is overexpressed in plants under abiotic and biotic stress conditions that mediate oxidative stress. to study its biological role and its ability to confer stress resistance in plants, we tried to obtain transgenic plants overexpressing citrus (citrus sinensis) phgpx (cit-phgpx). all attempts to obtain regenerated plants expressing this enzyme constitutively failed. however, when the enzyme's catalytic activity was abolished by active sit ... | 2010 | 20530511 |
| effect of liberibacter infection (huanglongbing or "greening" disease) of citrus on orange juice flavor quality by sensory evaluation. | some anecdotal reports suggest that infection of citrus trees with candidatus liberibacter asiaticus (las), the suspected causal agent of huanglongbing (hlb) disease, imparts off flavor to orange juice. it is of interest to the industry to know how las infection affects juice quality with respect to cultivar, maturity, or processing method. hamlin, midsweet, and valencia oranges were harvested over 2 y from trees that tested negative (las-) or positive (las+) for las from different groves and in ... | 2010 | 20546425 |
| evaluation of in vitro activity of essential oils against trypanosoma brucei brucei and trypanosoma evansi. | essential oils (eos) from cymbopogon citratus (cc), eucalyptus citriodora (ec), eucalyptus camaldulensis (ed), and citrus sinensis (cs) were obtained by hydrodistillation process. the eos were evaluated in vitro for activity against trypanosoma brucei brucei (tbb) and trypanosoma evansi (t. evansi). the eos were found to possess antitrypanosomal activity in vitro in a dose-dependent pattern in a short period of time. the drop in number of parasite over time was achieved doses of 0.4 g/ml, 0.2 g/ ... | 2010 | 20700425 |
| early events of citrus greening (huanglongbing) disease development at the ultrastructural level. | citrus greening (huanglongbing [hlb]) is one of the most destructive diseases of citrus worldwide. the causal agent of hlb in florida is thought to be 'candidatus liberibacter asiaticus'. understanding of the early events in hlb infection is critical for the development of effective measures to control the disease. in this work, we conducted cytopathological studies by following the development of the disease in citrus trees graft inoculated with 'ca. l. asiaticus'-containing material under gree ... | 2010 | 20701493 |
| effect of ploidy increase on transgene expression: example from citrus diploid cybrid and allotetraploid somatic hybrid expressing the egfp gene. | polyploidization is an important speciation mechanism for all eukaryotes, and it has profound impacts on biodiversity dynamics and ecosystem functioning. green fluorescent protein (gfp) has been used as an effective marker to visually screen somatic hybrids at an early stage in protoplast fusion. we have previously reported that the intensity of gfp fluorescence of regenerated embryoids was also an early indicator of ploidy level. however, little is known concerning the effects of ploidy increas ... | 2010 | 20734092 |
| shedding light on the role of photosynthesis in pathogen colonization and host defense. | the role of photosynthesis in plant defense is a fundamental question awaiting further molecular and physiological elucidation. to this end we investigated host responses to infection with the bacterial pathogen xanthomonas axonopodis pv. citri, the pathogen responsible for citrus canker. this pathogen encodes a plant-like natriuretic peptide (xacpnp) that is expressed specifically during the infection process and prevents deterioration of the physiological condition of the infected tissue. prot ... | 2010 | 20798833 |
| involvement of inducible nitric oxide synthase and cyclooxygenase-2 in the anti-inflammatory effects of a red orange extract in human chondrocytes. | in the present study, a complex of compounds (red orange complex, roc), obtained from three red orange varieties (citrus sinensis varieties: moro, tarocco and sanguinello), containing cyanidin glycosides, hydroxycinnamic acids, flavanone glycosides and ascorbic acid, was screened to discover new lead compounds in the suppression of the production of key molecules released during inflammatory events in interleukin-1beta (il-beta) stimulated human primary chondrocytes. the expression of inducible ... | 2010 | 20812134 |
| antiinflammatory effects of a red orange extract in human keratinocytes treated with interferon-gamma and histamine. | red oranges are an important component of the so-called mediterranean diet and they have been used by traditional medicine for their health protective properties, particularly to heal sore throat and cough, suggesting an interesting antiinflammatory activity. the purpose of this study was to evaluate the antiinflammatory activity of a red orange (citrus sinensis varieties: moro, tarocco, sanguinello) complex (roc), characterized by high levels of anthocyanins, flavanones, hydroxycinnamic acids a ... | 2010 | 19655299 |
| targeting excessive free radicals with peels and juices of citrus fruits: grapefruit, lemon, lime and orange. | a comparative study between the antioxidant properties of peel (flavedo and albedo) and juice of some commercially grown citrus fruit (rutaceae), grapefruit (citrus paradisi), lemon (citrus limon), lime (citrusxaurantiifolia) and sweet orange (citrus sinensis) was performed. different in vitro assays were applied to the volatile and polar fractions of peels and to crude and polar fraction of juices: 2,2-diphenyl-1-picrylhydrazyl (dpph) radical scavenging capacity, reducing power and inhibition o ... | 2010 | 19770018 |
| blood orange juice inhibits fat accumulation in mice. | to analyze the effect of the juice obtained from two varieties of sweet orange (citrus sinensis l. osbeck), moro (a blood orange) and navelina (a blond orange), on fat accumulation in mice fed a standard or a high-fat diet (hfd). | 2010 | 20029381 |
| different roles of functional residues in the hydrophobic binding site of two sweet orange tau glutathione s-transferases. | glutathione s-transferases (gsts) catalyze the conjugation of glutathione to hydrophobic compounds, contributing to the metabolism of toxic chemicals. in this study, we show that two naturally occurring tau gsts (gstus) exhibit distinctive kinetic parameters towards 1-chloro-2,4-dinitrobenzene (cdnb), although they differ only in three amino acids (arg89, glu117 and ile172 in gstu1 are replaced by pro89, lys117 and val172 in gstu2). in order to understand the effects of the single mismatched res ... | 2010 | 19954490 |
| a comparison of volatile components of setomi with its parent cultivars. | the citrus fruit setomi is a hybrid of yoshiura ponkan (citrus reticulate blanco) and kiyomi (citrus unshiu mrcov. x citrus sinensis osbeck). the essential oils from the peel of setomi and its parent cultivars were obtained by a simultaneous distillation extraction technique. comparing the essential oils of setomi and its parent cultivars, it was found that the oil in the peel of setomi consisted of characteristic aroma components from each parent cultivar. the principal component analysis of da ... | 2010 | 20208376 |
| anxiolytic-like effect of sweet orange aroma in wistar rats. | aromatherapy is the use of essential oils as an alternative treatment for medical purposes. despite the lack of sufficient scientific proof, it is considered a holistic complementary therapy employed to enhance comfort and decrease distress. citrus fragrances have been particularly used by aromatherapists for the treatment of anxiety symptoms. based on this claim, the present study investigated the effects of citrus sinensis (sweet orange) essential oil on wistar, male rats evaluated in the elev ... | 2010 | 20211673 |
| distribution and biological role of the oligopeptide-binding protein (oppa) in xanthomonas species. | in this study we investigated the prevalence of the oppa gene, encoding the oligopeptide binding protein (oppa) of the major bacterial oligopeptide uptake system (opp), in different species of the genus xanthomonas. the oppa gene was detected in two xanthomonas axonopodis strains among eight tested xanthomonas species. the generation of an isogenic oppa-knockout derivative of the xac 306 strain, showed that the oppa protein neither plays a relevant role in oligopeptide uptake nor contributes to ... | 2010 | 21637492 |
| insecticidal activity against bemisia tabaci biotype b of peel essential oil of citrus sinensis var. pear and citrus aurantium cultivated in northeast brazil. | the fumigant action of peel essential oils of citrus sinensis var. pear (pear orange = po) and c. aurantium (bitter orange = bo) from the northeast of brazil were evaluated against bemisia tabaci biotype b and compared with eugenol as a positive control. the oil concentration in the po at 8.5 microl/l of air caused 97% mortality, while the oil concentration of bo at 9.5 microl/l of air caused 99% mortality. however, the lc50 estimates for both oils (lc50 = 3.80 microl/l of air for po and lc50 = ... | 2010 | 21213990 |
| distribution of aroma volatile compounds in tangerine hybrids and proposed inheritance. | with the desirable combination of sugars and acids, volatile compounds contribute to the essential organoleptic attributes of citrus. this study evaluated the aroma volatiles of 20 tangerine hybrids of the university of florida breeding program. volatiles were sampled from hand-squeezed juice by headspace solid-phase microextraction (spme), and analyzed by gas chromatography-mass spectrometry. principal component analysis (pca) and cluster analysis (ca) were used to find similarities among sampl ... | 2010 | 21218478 |
| azteca barbifex forel (hymenoptera: formicidae): potential pest of citrus crops in eastern amazon. | damage caused by the ant azteca barbifex (forel) was identified in orange trees (citrus sinensis), in capitão poço county, guamá microregion, pará state. the damage caused by the scraping of stems and branches lead to reduction in yield with subsequent death of the plant. these characteristics indicate a. barbifex as a potential pest of citrus crops in the eastern region of amazon. | 2010 | 21271082 |
| liquid chromatographic profiles of major carotenoid esters in commercially processed california navel and valencia orange juice concentrates. | a procedure for establishing profiles of major carotenoid esters in commercially processed valencia and navel orange juice concentrates by reversed-phase liquid chromatography (lc) using sudan 1 as internal standard is described. the procedure involved conversion of 5,6-epoxides in heat concentrated citrus juices to more stable 5,8-epoxides by treatment of extracted carotenoids with hydrochloric acid followed by dual wavelength analyses at 400 and 465 nm using lc. the esters of 5,8-furanoids (au ... | 2010 | 3417817 |
| pharmacokinetics-based elucidation on disparity in clinical effectiveness between varieties of zhi zhu wan, a traditional chinese medical formula. | zhi zhu wan (zzw) is a classical chinese medical formulation used for the treatment of functional dyspepsia that attributed to spleen-deficiency syndrome. zzw contains atractylodes rhizome and fructus citrus immaturus, the later originates from both citrus aurantium l. (bzzw) and citrus sinensis osbeck (rzzw). the present study is designed to elucidate disparities in the clinical efficacy of two zzw varieties based on the pharmacokinetics of naringenin and hesperetin. mehtod: after oral administ ... | 2010 | 20184950 |
| comparative morpho-anatomical studies of the lesions caused by citrus leprosis virus on sweet orange. | the leprosis disease shows a viral etiology and the citrus leprosis virus is considered its etiologic agent. the disease may show two types of cytopatologic symptom caused by two virus: nuclear (cilv-n) and cytoplasmic (cilv-c) types. the aim of this study was to compare the morpho-anatomical differences in the lesions caused by leprosis virus-cytoplasmic and nuclear types in citrus sinensis (l.) osbeck 'pêra'. leaf and fruit lesions were collected in piracicaba/são paulo (cytoplasmic type) and ... | 2010 | 20563430 |
| identification of thermolabile pectin methylesterases from sweet orange fruit by peptide mass fingerprinting. | the multiple forms of the enzyme pectin methylesterase (pme) present in citrus fruit tissues vary in activity toward juice cloud-associated pectin substrates and, thus, in their impact on juice cloud stability and product quality. because the proteins responsible for individual pme activities are rarely identified by structural properties or correlated to specific pme genes, matrix-assisted laser desorption-ionization with tandem time-of-flight mass spectrometry (maldi-tof/tof ms) was investigat ... | 2010 | 21053908 |
| quality assessment of industrially processed fruit juices available in dhaka city, bangladesh. | this study was performed to evaluate the nutritional and microbiological quality of industrially processed packed fruit juices of mango (mangifera indica) and orange (citrus sinensis) from nine different manufacturing companies in dhaka city. the highest quantity of total sugar (17.62%) and reducing sugar (9.99%) was recorded in mango juices while the lowest in orange juices (10.41% and 2.24% respectively) of different companies. in this study, protein contents were comparatively higher in mango ... | 2010 | 22691996 |
| cloning, expression, purification and physical and kinetic characterization of the phosphoenolpyruvate carboxylase from orange (citrus sinensis osbeck var. valencia) fruit juice sacs. | phosphoenolpyruvate (pep) carboxylase (pepcase) from orange fruit juice sacs has been cloned and heterogously expressed in high yield. the purified recombinant enzyme displays properties typical of plant pepcase, including activation by sugar phosphates and inhibition by malate and citrate. malate inhibition is weak in the physiological ph range, and the enzyme is also poorly affected by glu and asp, known inhibitors of c(3) plants pepcases. however, it is strongly inhibited by citrate. orange f ... | 2010 | 21802611 |
| characterization of three terpenoid glycosyltransferase genes in 'valencia' sweet orange (citrus sinensis l. osbeck). | three putative terpenoid udp-glycosyltransferase (ugt) genes, designated csugt1, csugt2, and csugt3, were isolated and characterized in 'valencia' sweet orange (citrus sinensis l. osbeck). csugt1 consisted of 1493 nucleotides with an open reading frame encoding 492 amino acids, csugt2 consisted of 1727 nucleotides encoding 504 amino acids, and csugt3 consisted of 1705 nucleotides encoding 468 amino acids. csugt3 had a 145 bp intron at 730-874, whereas csugt1 and csugt2 had none. the three deduce ... | 2010 | 20962888 |
| comprehensive analysis of expressed sequence tags from the pulp of the red mutant 'cara cara' navel orange (citrus sinensis osbeck). | expressed sequence tag (est) analysis of the pulp of the red-fleshed mutant 'cara cara' navel orange provided a starting point for gene discovery and transcriptome survey during citrus fruit maturation. interpretation of the est datasets revealed that the mutant pulp transcriptome held a high section of stress responses related genes, such as the type iii metallothionein-like gene (6.0%), heat shock protein (2.8%), cu/zn superoxide dismutase (0.8%), late embryogenesis abundant protein 5 (0.8%), ... | 2010 | 20883438 |
| synergistic and antagonistic interactions of phenolic compounds found in navel oranges. | phenolic compounds are known to have antioxidant and antimicrobial properties. these properties may be useful in the preservation of foods or beverages. the interactive antioxidant capacity of phenolic compounds within foods has not been well explored. interactions of individual phenolic compounds (chlorogenic acid, hesperidin, luteolin, myricetin, naringenin, p-coumaric acid, and quercetin) at the concentrations found in navel oranges (citrus sinensis) were analyzed for their antioxidant capaci ... | 2010 | 20722912 |
| chemical constituents of citrus sinensis var. shukri from pakistan. | the variety 'shukri' is a new hybrid of citrus sinensis and is frequently grown for its sweet edible fruits in the southern part of pakistan. the leaves of this hybrid variety have been investigated in search of secondary metabolites for the first time. as a result of chromatographic analysis of the methanolic extract, a new ceramide along with a flavonone glycoside and two steroids has been isolated, which were spectroscopically characterized as (e)-n-(1,3,4,5-tetrahydroxyhexadecan-2-yl)dec-4-e ... | 2010 | 20706908 |
| timing of the inhibitory effect of fruit on return bloom of 'valencia' sweet orange (citrus sinensis (l.) osbeck). | in citrus the inhibitory effect of fruit on flower formation is the main cause of alternate bearing. although there are some studies reporting the effect on flowering of the time of fruit removal in a well-defined stage of fruit development, few have investigated the effect throughout the entire fruit growth stage from early fruitlet growth to fruit maturity. the objective of this study was to determine the phenological fruit developmental stage at which the fruit begins its inhibitory effect on ... | 2010 | 20564309 |
| physicochemical and molecular analysis of cell wall metabolism between two navel oranges (citrus sinensis) with different mastication traits. | fj72-1 navel orange and its bud mutant fjwc exhibit differences in melting texture character which are influenced mostly by cell wall metabolism. here we compared the contents of water soluble pectin (wsp), protopectin, total pectin (tp), cellulose, and hemicellulose, activities of polygalaturonase (pg), pectin methylesterase (pme), pectate lyases (pl), cellulase (cel) and gene expression levels of pg, pme, pl and cel between the two cultivars. | 2010 | 20549800 |
| [study on synchronous correlation between fruit characteristic spectrum and the parameter of internal quality for hamlin sweet orange fruit]. | the purpose of our study is to study the relationship between the reflective spectrum of fruit and the internal quality of the orange fruit and find the suitable mark spectrum that can synchronously measure the several fruit quality index at same time to lay a foundation for the development of a rapid and nondestructive field fruit quality analysis technique by analyzing the visible-near infrared spectrum. mature hamlin orange (citrus sinensis (l)cv. hamlin sweet orange) fruits were individually ... | 2010 | 20545159 |
| proteome from lemon fruit flavedo reveals that this tissue produces high amounts of the cit s1 germin-like isoforms. | a multistep procedure has been developed and applied to extract and purify proteins from lemon fruit flavedo. 2de, lc-esi-ms/ms, and bioinformatics were used to detect the high abundance of the germin-like glycoprotein cit s1, a powerful allergen in humans. peptide alignments against citrus est repositories gave the best scores with the c. sinensis cdna (gi|188354270/ey710037), annotated as unknown sweet orange fruit protein; additional blast of peptides against ncbi databases gave high sequence ... | 2010 | 20499937 |
| free and bound cinnamic acid derivatives in corsica sweet blond oranges. | total determination of cinnamic acids (ca), including hydroxycinnamic acid derivatives is generally not accurate since, during hydrolysis, a possible degradation of dihydroxy ca such as caffeic acid could occur. evaluations of ca (ferulic, p-coumaric, sinapic, cinnamic and caffeic acids) before and after hydrolysis have been undertaken using standards and either with or without addition of ascorbic acid and edta. the method was then applied to the determination of free and bound ca in five blond ... | 2010 | 20420324 |
| discovery and comparative profiling of micrornas in a sweet orange red-flesh mutant and its wild type. | red-flesh fruit is absent from common sweet orange varieties, but is more preferred by consumers due to its visual attraction and nutritional properties. our previous researches on a spontaneous red-flesh mutant revealed that the trait is caused by lycopene accumulation and is regulated by both transcriptional and post-transcriptional mechanisms. however, the knowledge on post-transcriptional regulation of lycopene accumulation in fruits is rather limited so far. | 2010 | 20398412 |
| light controls phospholipase a2alpha and beta gene expression in citrus sinensis. | the low-molecular weight secretory phospholipase a2alpha (csspla2alpha) and beta (cspla2beta) cloned in this study exhibited diurnal rhythmicity in leaf tissue of citrus sinensis. only csspla2alpha displayed distinct diurnal patterns in fruit tissues. csspla2alpha and cspla2beta diurnal expression exhibited periods of approximately 24 h; csspla2alpha amplitude averaged 990-fold in the leaf blades from field-grown trees, whereas cspla2beta amplitude averaged 6.4-fold. diurnal oscillation of csspl ... | 2010 | 20388744 |
| influence of different organic fertilizers on quality parameters and the delta(15)n, delta(13)c, delta(2)h, delta(34)s, and delta(18)o values of orange fruit (citrus sinensis l. osbeck). | to investigate the influence of different types of fertilizers on quality parameters, n-containing compounds, and the delta(15)n, delta(13)c, delta(2)h, delta (34)s, and delta(18)o values of citrus fruit, a study was performed on the orange fruit cv. 'valencia late' (citrus sinensis l. osbeck), which was harvested in four plots (three organic and one conventional) located on the same farm. the results demonstrated that different types of organic fertilizers containing the same amount of nitrogen ... | 2010 | 20184327 |
| development of a small scale orange juice extractor. | a small scale motorized orange juice extractor was designed and fabricated, using locally-available construction materials. the essential components of the machine include feeding hopper, top cover, worm shaft, juice sieve, juice collector, waste outlet, transmission belt, main frame, pulleys and bearings. in operation, the worm shaft conveys, crushes, presses and squeezes the fruit to extract the juice. the juice extracted is filtered through the juice sieve into juice collector while the resid ... | 2010 | 23572610 |
| biochemical properties of alpha-amylase from peel of citrus sinensis cv. abosora. | alpha-amylase activity was screened in the peel, as waste fruit, of 13 species and cultivars of egyptian citrus. the species citrus sinensis cv. abosora had the highest activity. alpha-amylase ai from abosora peel was purified to homogeneity using anion and cation-exchange, and gel filtration chromatographies. molecular weight of alpha-amylase ai was found to be 42 kda. the hydrolysis properties of alpha-amylase ai toward different substrates indicated that corn starch is the best substrate. the ... | 2010 | 19941088 |
| use of a custom array to study differentially expressed genes during blood orange (citrus sinensis l. osbeck) ripening. | a flesh-specific oligonucleotide custom array was designed to study gene expression during blood orange ripening. the array included 301 probes derived from a subtracted ssh library, a cdna-aflp collection, and a set of regulatory genes from the harvest citrus database. the custom array was hybridized using samples of moro, a pigmented cultivar, and cadenera, a common cultivar, at three different ripening stages: the immature phase, the halfway point of maturation (corresponding to the start of ... | 2010 | 19864041 |
| relationships between xylem anatomy, root hydraulic conductivity, leaf/root ratio and transpiration in citrus trees on different rootstocks. | the aim of the study was to determine the extent in which leaf and whole plant transpiration (tp) were influenced by root hydraulic conductance (k(r)), leaf to root ratio and leaf mass. also, the relationships between the anatomic characteristics of roots and k(r) were investigated. to this end, 9-month-old seedlings of the citrus rootstocks cleopatra mandarin (cm), poncirus trifoliata (pt), and their hybrids forner-alcaide no 5 (fa-5) and forner-alcaide no 13 (fa-13) and 15-month-old trees of v ... | 2010 | 20088906 |
| [research on zinc content in leaf of olinda valencia orange using visible near infrared spectroscopy model]. | olinda valencia orange leaves dry powder-like were taken as sample, and chemical analysis combined with technology of visible near-infrared spectroscopy (vis/nirs) was used, through the treatment process of second derivative spectrum of samples of the original spectrum and denoising (noise). meanwhile, method of partial least squares (pls) and cross-validation were used to establish maths model of zn concentration which applying band combination composited by 400-500 and 1201-1300 nm of characte ... | 2010 | 21284155 |
| [application of aas to detecting the influence of railway on the soil of navel orange garden]. | railway transportation has boosted the economy of railway road area, meanwhile it brings some undesirable impacts on the environment of the railway road area. the quality of the fruits is directly related with the elements of the soil, so understanding the element contents of soil in navel oranges garden in the vicinity of railway is meaningful to the security of agriculture products and ecological conditions in the areas surrounding the railways. as a favorite fruit, navel orange is widely plan ... | 2010 | 20707171 |
| [first supplement to the bibliography on citrus sinensis]. | 2009 | 13125380 | |
| comparative proteomics of a lycopene-accumulating mutant reveals the important role of oxidative stress on carotenogenesis in sweet orange (citrus sinensis [l.] osbeck). | a spontaneous sweet orange (citrus sinenesis [l.] osbeck) mutant 'hong anliu' is of high value due to lycopene accumulation in the pulp. in this study, we analyzed the proteomic alterations in the pulp of 'hong anliu' versus its wild type (wt) at four maturing stages by using 2-de combined with maldi-tof-tof ms. among the 74 differentially expressed proteins identified, the majority are predicted to be involved in stress response, carbohydrate/energy metabolism and regulation, or protein fate, m ... | 2009 | 19834898 |
| proteome analysis of citrus sinensis l. (osbeck) flesh at ripening time. | a combination of 2-de and lc-msms approaches was used to identify the differentially expressed proteome of a pigmented sweet orange (citrus sinensis, cv. moro) in comparison with a common cultivar (cadenera) at ripening time. the comparison of the protein patterns of moro and cadenera showed 64 differential expressed protein spots. fifty-five differentially expressed proteins were identified. proteins were classified according to their putative function and known biosynthetic pathways. most of t ... | 2009 | 19775598 |
| identification, recombinant expression, and biochemical characterization of a flavonol 3-o-glucosyltransferase clone from citrus paradisi. | glucosylation is a predominant flavonoid modification reaction affecting the solubility, stability, and subsequent bioavailability of these metabolites. flavonoid glycosides affect taste characteristics in citrus making the associated glucosyltransferases particularly interesting targets for biotechnology applications in these species. in this work, a citrus paradisi glucosyltransferase gene was identified, cloned, and introduced into the pet recombinant protein expression system utilizing prime ... | 2009 | 19733370 |
| phellodendron and citrus extracts benefit joint health in osteoarthritis patients: a pilot, double-blind, placebo-controlled study. | the objective of this clinical study was to assess the potential benefit of a dietary supplement, np 06-1, on joint health in overweight and normal weight adults diagnosed with osteoarthritis. | 2009 | 19682376 |
| seasonal population dynamics of homalodisca vitripennis (hemiptera: cicadellidae) in sweet orange trees maintained under continuous deficit irrigation. | a 2-yr study was conducted in a citrus orchard (citrus sinensis l. osbeck cultivar valencia) to determine the influence of plant water stress on the population dynamics of glassy-winged sharpshooter, homalodisca vitripennis (germar). experimental treatments included irrigation at 100% of the crop evapotranspiration rate (et(c)) and continuous deficit-irrigation regimens at 80 and 60% et(c). microclimate and plant conditions monitored included temperature and humidity in the tree canopy, leaf sur ... | 2009 | 19610409 |
| pattern recognition and genetic algorithms for discrimination of orange juices and reduction of significant components from headspace solid-phase microextraction. | orange (citrus sinensis l.) juice comprises a complex mixture of volatile components that are difficult to identify and quantify. classification and discrimination of the varieties on the basis of the volatile composition could help to guarantee the quality of a juice and to detect possible adulteration of the product. | 2009 | 19609881 |
| thermal degradation kinetics of anthocyanins from blood orange, blackberry, and roselle using the arrhenius, eyring, and ball models. | anthocyanin stability was assessed over temperatures ranging from 30 to 90 degrees c for seven products: blood orange juice [citrus sinensis (l.) osbeck]; two tropical highland blackberry juices (rubus adenotrichus schlech.), one with high content and the other with low content of suspended insoluble solids (sis); and four roselle extracts (hibiscus sabdariffa l.). the blackberry juice showed the highest content of anthocyanins with 1.2 g/l (two times less in the roselle extracts and 12 times le ... | 2009 | 19545116 |
| efficacy of essential oils from edible plants as insecticides against the house fly, musca domestica l. | the compositions of 12 essential oils (eos) obtained by hydrodistillation of edible fruits and herbs were analyzed by gas chromatography/mass spectroscopy (gc/ms). the insecticidal activity of each oil against the house fly musca domestica was evaluated by placing flies in a glass jar with a screw cap that held a piece of eo-treated cotton yarn. the dose necessary to kill 50% of flies (lc(50)) in 30 min was determined at 26 +/- 1 degrees c. twelve eos and 17 individual terpenes were assayed agai ... | 2009 | 19471213 |
| gene isolation and expression analysis of two distinct sweet orange [citrus sinensis l. (osbeck)] tau-type glutathione transferases. | glutathione s-transferases (gsts) represent a multifunctional family of enzymes grouped into four main classes (tau, phi, theta, and zeta) conjugating endobiotic and xenobiotic compounds to glutathione. in plants, this is considered to be a crucial step in the detoxification process as the s-glutathionylated metabolites are tagged for vacuolar sequestration. in this work, we have isolated two glutathione s-transferases belonging to the tau class gsts from sweet orange leaves. the cdna clones con ... | 2009 | 19422890 |
| influence of spray equipment and water volume on coverage of citrus and control of citricola scale, coccus pseudomagnoliarum (hemiptera: coccidae) with mineral oil. | a trial was conducted in a commercial citrus sinensis l. variety 'washington' navel orange orchard to compare the coverage and efficacy against citricola scale coccus pseudomagnoliarum (kuwana) (hemiptera: coccidae) of 45.5 liters/ha of an nc24 agricultural mineral oil treatment applied by two different methods: a conventional air blast sprayer and a rotary atomizer. three water volumes (2,340, 4,680, and 7,020 liters/ha) were applied with the air blast sprayer to determine the optimal spray vol ... | 2009 | 19253648 |
| transcriptome analysis of a spontaneous mutant in sweet orange [citrus sinensis (l.) osbeck] during fruit development. | bud mutations often arise in citrus. the selection of mutants is one of the most important breeding channels in citrus. however, the molecular basis of bud mutation has rarely been studied. to identify differentially expressed genes in a spontaneous sweet orange [c. sinensis (l.) osbeck] bud mutation which causes lycopene accumulation, low citric acid, and high sucrose in fruit, suppression subtractive hybridization and microarray analysis were performed to decipher this bud mutation during frui ... | 2009 | 19218315 |
| ammonium transport and citamt1 expression are regulated by n in citrus plants. | citrus seedlings (citrus sinensis l. osbeck x poncirus trifoliata blanco) were used to describe the effects of different n treatments on the nh4+ influx mediated by high- and low-affinity transport systems (hats and lats, respectively) and citamt1 gene expression. results show that citrus plants favor nh4+ over no3- influx mediated by hats and lats when both n sources are present in the nutrient solution and citrus plants display a much higher capacity to take up nh4+ than no3-. furthermore, nh4 ... | 2009 | 19023591 |