Publications

efficacy of herbal essential oils as insecticide against aedes aegypti (linn.), culex quinquefasciatus (say) and anopheles dirus (peyton and harrison).	the essential oils of cananga odorata (ylang ylang), citrus sinensis (orange), cymbopogon citratus (lemongrass), cymbopogon nardus (citronella grass), eucalyptus citriodora (eucalyptus), ocimum basilicum (sweet basil) and syzygium aromaticum (clove), were tested for their insecticide activity against aedes aegypti, culex quinquefasciatus and anopheles dirus using the who standard susceptibility test. these were applied in soybean oil at dose of 1%, 5% and 10% (w/v). c. citratus had the kt, value ...	2011	22299433
melanogenesis of murine melanoma cells induced by hesperetin, a citrus hydrolysate-derived flavonoid.	melanogenesis is a complex process that modulates skin pigmentation to defend photodamage. citrus is the most widely produced fruit crop in the world. people ingest various citrus fruits in their common diets. in the present study, the acid-hydrolyzed and un-hydrolyzed extracts of orange-type citrus fruits were subjected to analyze flavonoid compositions and assess their effects on melanin synthesis in murine b16-f10 melanoma cells. the acid-hydrolyzed extracts of citrus sinensis, c. reticulata, ...	2012	22266363
efficacy of killer yeasts in the biological control of penicillium digitatum on tarocco orange fruits (citrus sinensis).	killer saccharomyces cerevisiae and wickerhamomyces anomalus yeast strains were tested as biocontrol agents against penicillium digitatum, one the most important causes of postharvest decay in orange fruits. w. anomalus, grown on acidified medium, demonstrated micocinogenic activity against p. digitatum, as indicated by large inhibition halos and hyphal damage resulting from β-glucanase activity. oranges that had been deliberately inoculated with pathogens were protected from decay by w. anomalu ...	2012	22265304
electronic nose application for determination of penicillium digitatum in valencia oranges.	penicillium digitatum and penicillium italicum are responsible for one the most serious diseases occurring during storage of citrus fruits. its early detection allows a relevant increase in shelf life, and in situ monitoring of fungal infections represents a very efficient tool to improve storage quality. in the case of metabolic alterations due to physiological or fungal pathologies, olfactometric analysis allows the detection of specific volatile biomarkers, thus providing an effective tool fo ...	2012	22261834
snp mining in c. clementina bac end sequences; transferability in the citrus genus (rutaceae), phylogenetic inferences and perspectives for genetic mapping.	with the increasing availability of est databases and whole genome sequences, snps have become the most abundant and powerful polymorphic markers. however, snp chip data generally suffers from ascertainment biases caused by the snp discovery and selection process in which a small number of individuals are used as discovery panels. the ongoing international citrus genome consortium sequencing project of the highly heterozygous clementine and sweet orange genomes will soon result in the release of ...	2012	22233093
transcriptome changes during fruit development and ripening of sweet orange (citrus sinensis).	the transcriptome of the fruit pulp of the sweet orange variety anliu (wt) and that of its red fleshed mutant hong anliu (mt) were compared to understand the dynamics and differential expression of genes expressed during fruit development and ripening.	2012	22230690
characterization and comparison of volatile constituents of juice and peel from clementine, mandarin and their hybrids.	the volatile compositions of juice and peel of clementine (citrus reticulata x citrus sinensis var. commune), mandarin (citrus reticulata blanco var. willow leaf) and their hybrids were analyzed by headspace solid-phase extraction (hs-spme) coupled with gas chromatography and either a flame ionization detector (fid) or a mass spectrometer (ms). the major compounds of the volatile fractions of clementine and mandarin were limonene and limonene/gamma-terpinene, respectively. the volatile compositi ...	2011	22164792
comparative use of indel and ssr markers in deciphering the interspecific structure of cultivated citrus genetic diversity: a perspective for genetic association studies.	genetic stratification associated with domestication history is a key parameter for estimating the pertinence of genetic association study within a gene pool. previous molecular and phenotypic studies have shown that most of the diversity of cultivated citrus results from recombination between three main species: c. medica (citron), c. reticulata (mandarin) and c. maxima (pummelo). however, the precise contribution of each of these basic species to the genomes of secondary cultivated species, su ...	2012	22160318
neurotrophic effect of citrus 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone: promotion of neurite outgrowth via camp/pka/creb pathway in pc12 cells.	5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone (5-oh-hxmf), a hydroxylated polymethoxyflavone, is found exclusively in the citrus genus, particularly in the peels of sweet orange. in this research, we report the first investigation of the neurotrophic effects and mechanism of 5-oh-hxmf in pc12 pheochromocytoma cells. we found that 5-oh-hxmf can effectively induce pc12 neurite outgrowth accompanied with the expression of neuronal differentiation marker protein growth-associated protein-43(gap-43). 5- ...	2011	22140566
insecticidal evaluation of essential oils of citrus sinensis l. (myrtales: myrtaceae) against housefly, musca domestica l. (diptera: muscidae).	the housefly, musca domestica l., is one of the most common insects, associated with vectoring of various etiological agents. in order to search for effective control agent, the essential oil of sweet orange [citrus sinensis (l.) osbeck] was evaluated for its insecticidal activity against the larvae and pupae of housefly using contact toxicity and fumigation bioassays. in the contact toxicity assay, lethal concentration, lc(50) of c. sinensis essential oil against housefly larvae, varied between ...	2012	22127387
interaction of hesperetin glucuronide conjugates with human bcrp, mrp2 and mrp3 as detected in membrane vesicles of overexpressing baculovirus-infected sf9 cells.	the citrus flavonoid hesperetin (4'-methoxy-3',5,7-trihydroxyflavanone) is the aglycone of hesperidin, the major flavonoid present in sweet oranges. hesperetin 7-o-glucuronide (h7g) and hesperetin 3'-o-glucuronide (h3'g) are the two most abundant metabolites of hesperetin in vivo. in this study, their interaction with specific abc transporters, believed to play a role in the disposition and bioavailability of hesperetin, was studied using sf9 membranes from cells overexpressing human bcrp (abcg2 ...	2011	22083890
Regulation of carotenoid accumulation and the expression of carotenoid metabolic genes in citrus juice sacs in vitro.	In the present study, to investigate the mechanisms regulating carotenoid accumulation in citrus, a culture system was set up in vitro with juice sacs of three citrus varieties, Satsuma mandarin (Citrus unshiu Marc.), Valencia orange (Citrus sinensis Osbeck), and Lisbon lemon (Citrus limon Burm.f.). The juice sacs of all the three varieties enlarged gradually with carotenoid accumulation. The changing patterns of carotenoid content and the expression of carotenoid metabolic genes in juice sacs i ...	2011	21994171
glycaemic response to some commonly eaten fruits in type 2 diabetes mellitus.	it is not known which of the commonly consumed fruits in nigeria are suitable for persons with diabetes mellitus especially with regards to the attendant plasma glucose response (pgr) to consumption of such fruits.	2011	21984455
metal analysis in citrus sinensis fruit peel and psidium guajava leaf.	the determination of metal traces is very important because they are involved in biological cycles and indicate high toxicity. the objective of the present study is to measure the levels of heavy metals and mineral ions in medicinally important plant species, citrus sinensis and psidium guajava. this study investigates the accumulation of copper (cu), zinc (zn), cadmium (cd), aluminum (al), mercury (hg), arsenic (as), selenium (se) and inorganic minerals like calcium (ca) and magnesium (mg) in c ...	2011	21976824
Comparison of ascorbate metabolism in fruits of two citrus species with obvious difference in ascorbate content in pulp.	Citrus fruit is widely consumed and provides ascorbate for human health. The ascorbate content in pulp is generally higher in orange (Citrus sinensis Osb.) than in Satsuma mandarin (Citrus unshiu Marc.). However, what contributes to such difference is still unknown. In the present study, ascorbate accumulation, expression profiles of genes involved in L-galactose pathway and activity changes of enzymes related with L-ascorbic acid (AA) oxidation and recycling were investigated during fruit devel ...	2011	21925761
temperature thresholds and degree-day model for marmara gulosa (lepidoptera: gracillariidae).	the developmental thresholds for marmara gulosa guillén & davis (lepidoptera: gracillariidae) were investigated in the laboratory by using 17, 21, 25, 29, and 33 degrees c. the lowest mortality occurred in cohorts exposed to 25 and 29 degrees c. other temperatures caused >10% mortality primarily in egg and first and second instar sap-feeding larvae. linear regression analysis approximated the lower developmental threshold at 12.2 degrees c. high mortality and slow developmental rate at 33 degree ...	2011	21882694
cold susceptibility and disinfestation of bactrocera invadens (diptera: tephritidae) in oranges.	to develop a cold disinfestation treatment for the fruit fly bactrocera invadens drew, tsuruta & white (diptera: tephritidae) that is rapidly spreading across africa, research was conducted in nairobi, kenya, using flies from a laboratory culture and 'valencia' orange (citrus sinensis l. osbeck) as the host. the developmental rate of b. invadens in valencia oranges was determined at 28 degrees c, and the third instar was found to be the least susceptible of the egg and larval life stages to cold ...	2011	21882681
cold treatment of ceratitis capitata (diptera: tephritidae) in oranges using a larval endpoint.	south africa currently exports fresh citrus (citrus spp.) fruit to japan using an in-transit cold treatment protocol of 14 d or 12 d at temperatures <0 degrees c for treatment of ceratitis capitata (wiedemann) (diptera: tephritidae) in 'clementine' mandarins (citrus reticulata blanco) and other citrus types, respectively. to reduce the risk of chilling injury with this treatment, research was conducted with temperatures >0 degrees c. earlier south african research had shown that young (6-d-old) ...	2011	21882680
evaluation of effects of zingiber officinale on salivation in rats.	there are some herbal plants in iranian traditional system of medicine which are believed to be excellent remedies to alleviate the symptoms of xerostomia. the aim of the present study was to evaluate the effect of systemic administration of seven different herbal extracts on the rate of salivation in rats. the extracts of 7 herbs; zingiber officinale roscoe (zingiberaceae), citrus sinensis (l.) osbeck (rutaceae), artemisia absinthium l. (asteraceae), cichorium intybus l. (asteraceae), pimpinell ...	2011	21874635
cloning, purification and characterization of a 90kda heat shock protein from citrus sinensis (sweet orange).	protein misfolding is stimulated by stress, such as heat, and heat shock proteins (hsps) are the first line of defense against these undesirable situations. plants, which are naturally sessile, are perhaps more exposed to stress factors than some other organisms, and consequently, the role of hsps is crucial to maintain homeostasis. hsp90, because of its key role in infection and other stresses, is targeted in therapies that improve plant production by increasing resistance to both biotic and ab ...	2012	21873074
mutations of β-tubulin codon 198 or 200 indicate thiabendazole resistance among isolates of penicillium digitatum collected from citrus in taiwan.	penicillium digitatum causes green mold on citrus, resulting in severe postharvest fruit decay and economic losses in many citrus-producing areas of the world. forty isolates of p. digitatum were cultured from citrus groves, packinghouses, and local markets in taiwan, and assessed quantitatively for their sensitivity to thiabendazole (tbz) fungicide. sensitivity assays using a 96-well microtiter plate revealed that, of 40 isolates examined, only one isolate collected from fruit produced in taiwa ...	2011	21868117
a proteomic analysis of the chromoplasts isolated from sweet orange fruits [citrus sinensis (l.) osbeck].	here, a comprehensive proteomic analysis of the chromoplasts purified from sweet orange using nycodenz density gradient centrifugation is reported. a gelc-ms/ms shotgun approach was used to identify the proteins of pooled chromoplast samples. a total of 493 proteins were identified from purified chromoplasts, of which 418 are putative plastid proteins based on in silico sequence homology and functional analyses. based on the predicted functions of these identified plastid proteins, a large propo ...	2011	21841170
controlling for sugar and ascorbic acid, a mixture of flavonoids matching navel oranges significantly increases human postprandial serum antioxidant capacity.	fruit and vegetable consumption reduces the risk for cardiovascular disease development. the postprandial state is an important contributor to chronic disease development. orange flavonoids may reduce postprandial oxidation. it was hypothesized that a mixture of orange flavonoids would reduce postprandial oxidation better than a single orange flavonoid or orange sugar and ascorbic acid, but not as well as orange juice, when consumed with a typical breakfast. a placebo-controlled crossover trial ...	2011	21840468
longevity of multiple species of tephritid (diptera) fruit fly parasitoids (hymenoptera: braconidae: opiinae) provided exotic and sympatric-fruit based diets.	while adult parasitic hymenoptera in general feed on floral and extrafloral nectars, hemipteran-honeydews and fluids from punctured hosts, diachasmimorpha longicaudata (ashmead), an old world opiine braconid introduced to tropical/subtropical america for the biological control of anastrepha spp. (tephritidae), can survive on fruit juices as they seep from injured fruit. an ability to exploit fruit juice would allow such a parasitoid to efficiently forage for hosts and food sources simultaneously ...	2011	21839085
comparative itraq proteome and transcriptome analyses of sweet orange infected by candidatus liberibacter asiaticus.	citrus huanglongbing (hlb) has been threatening citrus production worldwide. in this study, a comparative proteomic approach was applied to understand the pathogenic process of hlb in affected sweet orange leaves. using the isobaric tags for relative and absolute quantification (itraq) technique, we identified 686 unique proteins in the mature leaves of both mock-inoculated and diseased 'madam vinous' sweet orange plants. of the identified proteins, 20 and 10 were differentially expressed in lea ...	2011	21838733
comparative analysis of the volatile fraction of fruit juice from different citrus species.	the volatile composition of fruit from four citrus varieties (powell navel orange, clemenules mandarine, and fortune mandarine and chandler pummelo) covering four different species has been studied. over one hundred compounds were profiled after hs-spme-gc-ms analysis, including 27 esters, 23 aldehydes, 21 alcohols, 13 monoterpene hydrocarbons, 10 ketones, 5 sesquiterpene hydrocarbons, 4 monoterpene cyclic ethers, 4 furans, and 2 aromatic hydrocarbons, which were all confirmed with standards. th ...	2011	21818287
citrus essential oil of nigeria. part v: volatile constituents of sweet orange leaf oil (citrus sinensis).	the volatile oils extracted from leaves of eight cultivars of citrus sinensis (l) osbeck were comprehensively analysed by a combination of gc and gc-ms. fifty four constituents accounting for 82.3-98.2% were identified. sabinene (20.9-49.1%), delta-3-carene (0.3-14.3%), (e)-beta-ocimene (4.4-12.6%), linalool (3.7-11.1%) and terpinen-4-ol (1.7-12.5%) were the major constituents that are common to all the volatile oils. in addition, a cluster analysis was carried out and indicated at least four di ...	2011	21815430
cloning, expression, purification and physical and kinetic characterization of the phosphoenolpyruvate carboxylase from orange (citrus sinensis osbeck var. valencia) fruit juice sacs.	phosphoenolpyruvate (pep) carboxylase (pepcase) from orange fruit juice sacs has been cloned and heterogously expressed in high yield. the purified recombinant enzyme displays properties typical of plant pepcase, including activation by sugar phosphates and inhibition by malate and citrate. malate inhibition is weak in the physiological ph range, and the enzyme is also poorly affected by glu and asp, known inhibitors of c(3) plants pepcases. however, it is strongly inhibited by citrate. orange f ...	2010	21802611
[research on optimization of model for detecting sugar content of navel orange by online near infrared spectroscopy].	the objective of the present research was to optimize the model of sugar content in navel orange for improving the detection presicion by the online near infrared spectroscopy. the reference wavelength was chosen by coefficient of variation of the different wavelengths in the calibration set in the wavelength range of 700.28 - 933.79 nm. then the spectra were transformed into ratio specra. the absorbance and ration spectra were pretreated by different preprocessing methods. the models of sugar c ...	2011	21800571
to compare the efficacy of two kinds of zhizhu pills in the treatment of functional dyspepsia of spleen-deficiency and qi-stagnation syndrome: a randomized group sequential comparative trial.	in traditional chinese medicine (tcm) theory, functional dyspepsia (fd) can be divided into different syndromes according to different clinical symptoms and signs, and the most common one is spleen-deficiency and qi-stagnation syndrome that can be treated by chinese traditional patent medicine--two kinds of zhizhu pills, between which the primary difference in ingredients is that one contains immature orange fruit of citrus aurantium l.(ifca) and the other contains that of citrus sinensis osbeck ...	2011	21762493
chemical elements in organic and conventional sweet oranges.	this work focuses on the determination of chemical elements in sweet oranges of variety valencia produced under organic and conventional systems using instrumental neutron activation analysis and inductively coupled plasma mass spectrometry. the distribution of chemical elements was variable among the fruit parts with usually higher concentrations of br, ca, ce, k, la, na, rb, and sc in the peel. however, k, na, and rb also presented high values in the juice samples, while fe and zn were higher ...	2011	21735113
comparison of cell wall metabolism in the pulp of three cultivars of 'nanfeng' tangerine differing in mastication trait.	background: like sweet orange (citrus sinensis), tangerine (citrus reticulata) is another citrus crop grown widely throughout the world. however, whether it shares a common mechanism with sweet orange in forming a given mastication trait is still unclear. in this study, three 'nanfeng' tangerine cultivars, 'yangxiao-26' ('yx-26') with inferior mastication trait, elite 'yx-26' with moderate mastication trait and 'miguang' ('mg') with superior mastication trait, were selected to investigate the fo ...	2011	21732384
isolation, identification and sequence analysis of a thioredoxin h gene, a member of subgroup iii of h-type trxs from grape (vitis vinifera l. cv. askari).	thioredoxins (trxs) are small ubiquitous proteins which play a regulatory role in a variety of cellular processes. in contrast to other organisms, plants have a great number of trx types, consisting of six well-defined groups: f, m, x, and y in chloroplasts, o in mitochondria, and h mainly in cytosol. a full-length cdna, designated vvcxxs2, encoding trx h polypeptide was isolated and cloned from grape (vitis vinifera l. cv. askari) berries organ by reverse transcription polymerase chain reaction ...	2011	21732058
fiber concentrate from orange (citrus sinensis l.) bagase: characterization and application as bakery product ingredient.	orange is a tropical fruit used in the juice industry, yielding important quantities of by products. the objective of this work was to obtain a dietary fiber-rich orange bagasse product (dfrobp), evaluate its chemical composition and its use in the preparation of a bakery product (muffin). muffins containing two different levels of dfrobp were studied regarding chemical composition, in vitro starch digestibility, predicted glyceamic index and acceptability in a sensory test. dfrobp showed low fa ...	2011	21731434
ectopic expression of the p23 silencing suppressor of citrus tristeza virus differentially modifies viral accumulation and tropism in two transgenic woody hosts.	citrus tristeza virus (ctv), a phloem-restricted closterovirus infecting citrus, encodes three different silencing suppressors (p25, p20 and p23), one of which (p23) is a pathogenicity determinant that induces aberrations resembling ctv symptoms when expressed ectopically in transgenic citrus hosts. in this article, the effect of p23 ectopic expression on virus infection was examined in sweet orange (swo), a highly susceptible host, and sour orange (so), which severely restricts ctv cell-to-cell ...	2011	21726389
colonization of citrus seed coats by 'candidatus liberibacter asiaticus': implications for seed transmission of the bacterium.	huanglongbing is an economically damaging disease of citrus associated with infection by 'ca. liberibacter asiaticus'. transmission of the organism via infection of seeds has not been demonstrated but is a concern since some citrus varieties, particularly those used as rootstocks in commercial plantings are propagated from seed. we compared the incidence of detection of 'ca. liberibacter asiaticus' dna in individual fruit peduncles, seed coats, seeds and in germinated seedlings from 'sanguenelli ...	2011	21714779
limonoid content of sour orange varieties.	modern citrus cultivars are thought to have arisen from three parents: the pummelo, the mandarin, and citron. taxological and genetic data support that sweet and sour oranges share a common parentage. however, as their name suggests, the organoleptic properties of the fruit from these two families is distinctly different. analysis of the limonoid content of sour orange varieties has been limited.	2011	21681758
distribution and biological role of the oligopeptide-binding protein (oppa) in xanthomonas species.	in this study we investigated the prevalence of the oppa gene, encoding the oligopeptide binding protein (oppa) of the major bacterial oligopeptide uptake system (opp), in different species of the genus xanthomonas. the oppa gene was detected in two xanthomonas axonopodis strains among eight tested xanthomonas species. the generation of an isogenic oppa-knockout derivative of the xac 306 strain, showed that the oppa protein neither plays a relevant role in oligopeptide uptake nor contributes to ...	2010	21637492
the sucrose synthase-1 promoter from citrus sinensis directs expression of the β-glucuronidase reporter gene in phloem tissue and in response to wounding in transgenic plants.	interest in phloem-specific promoters for the engineering of transgenic plants has been increasing in recent years. in this study we isolated two similar, but distinct, alleles of the citrus sinensis sucrose synthase-1 promoter (cssus1p) and inserted them upstream of the β-glucuronidase (gus) gene to test their ability to drive expression in the phloem of transgenic arabidopsis thaliana and nicotiana tabacum. although both promoter variants were capable of conferring localized gus expression in ...	2011	21594624
tetraploidization events by chromosome doubling of nucellar cells are frequent in apomictic citrus and are dependent on genotype and environment.	background and aims polyploidy is a major component of plant evolution. the citrus gene pool is essentially diploid but tetraploid plants are frequently encountered in seedlings of diploid apomictic genotypes. the main objectives of the present study were to establish the origin of these tetraploid plants and to ascertain the importance of genotypic and environmental factors on tetraploid formation. methods tetraploid seedlings from 30 diploid apomictic genotypes were selected by flow cytometry ...	2011	21586529
partial dehydration and cryopreservation of citrus seeds.	three categories of seed storage behavior are generally recognized among plant species: orthodox, intermediate and recalcitrant. intermediate seeds cannot be stored in liquid nitrogen (ln) without a previous partial dehydration process. the water content (wc) of the seeds at the moment of immersion in ln must be regarded as the most critical factor in cryopreservation. the purpose of this study was to investigate the basis of the optimal hydration status for cryopreservation of citrus seeds: c. ...	2011	21538368
biosynthesis of silver nanoparticles using citrus sinensis peel extract and its antibacterial activity.	biosynthesis of silver nanoparticles (agnps) was achieved by a novel, simple green chemistry procedure using citrus sinensis peel extract as a reducing and a capping agent. the effect of temperature on the synthesis of silver nanoparticles was carried out at room temperature (25°c) and 60°c. the successful formation of silver nanoparticles has been confirmed by uv-vis, ftir, xrd, edax, fesem and tem analysis and their antibacterial activity against escherichia coli, pseudomonas aeruginosa (gram- ...	2011	21536485
terpene down-regulation in orange reveals the role of fruit aromas in mediating interactions with insect herbivores and pathogens.	plants use volatile terpene compounds as odor cues for communicating with the environment. fleshy fruits are particularly rich in volatiles that deter herbivores and attract seed dispersal agents. we have investigated how terpenes in citrus fruit peels affect the interaction between the plant, insects, and microorganisms. because limonene represents up to 97% of the total volatiles in orange (citrus sinensis) fruit peel, we chose to down-regulate the expression of a limonene synthase gene in ora ...	2011	21525333
host status of grapefruit and valencia oranges for anastrepha serpentina and anastrepha ludens (diptera: tephritidae).	anastrepha serpentina (wiedemann) (diptera: tephritidae) is sporadically captured in the rio grande valley of texas. although its preferred hosts are in the sapotaceae family, several varieties of citrus, including grapefruit and oranges are listed as alternate hosts. although mexican fruit fly, anastrepha ludens (loew), is known to be a major pest of citrus, doubt exists as to the status of citrus as a breeding host for a. serpentina. to evaluate the host status of commercial citrus for a. serp ...	2011	21510184
inhibition of tnf-α/ifn-γ induced rantes expression in hacat cell by naringin.	context: naringin is a bioflavonoid derivative and is predominantly found in citrus paradisi macf., citrus sinensis (linn.) osbeck, citrus unshiu marc., citrus reticulata blanco cv. nobilis, citrus tachibana (makino) tanaka, citrus junos sieb. ex tanaka (rutaceae), and related citrus species. it has anti-inflammatory effects that have been well-documented, but the mechanism is poorly characterized. objective: the effect of naringin on production of rantes (regulated upon activation normal t-cell ...	2011	21500970
determination of volatile, phenolic, organic acid and sugar components in a turkish cv. dortyol (citrus sinensis l. osbeck) orange juice.	orange flavour is the results of a natural combination of volatile compounds in a well-balanced system including sugars, acids and phenolic compounds. this paper reports the results of the first determination of aroma, organic acids, sugars, and phenolic components in dortyol yerli orange juices.	2011	21480267
ectopic expression of mdspds1 in sweet orange (citrus sinensis osbeck) reduces canker susceptibility: involvement of hôééoôéé production and transcriptional alteration.	enormous work has shown that polyamines are involved in a variety of physiological processes, but information is scarce on the potential of modifying disease response through genetic transformation of a polyamine biosynthetic gene.	2011	21439092
physical location of the carotenoid biosynthesis genes psy and β-lcy in capsicum annuum (solanaceae) using heterologous probes from citrus sinensis (rutaceae).	carotenoids are responsible for a range of fruit colors in different hot pepper (capsicum) varieties, from white to deep red. color traits are genetically determined by three loci, y, c1, and c2, which are associated with carotenogenic genes. although such genes have been localized on genetic maps of capsicum and anchored in lycopersicon and solanum, physical mapping in capsicum has been restricted to only a few clusters of some multiple copy genes. heterologous probes from single copy genes hav ...	2011	21425090
photosynthesis down-regulation precedes carbohydrate accumulation under sink limitation in citrus.	photosynthesis down-regulation due to an imbalance between sources and sinks in citrus leaves could be mediated by excessive accumulation of carbohydrates. however, there is limited understanding of the physiological role of soluble and insoluble carbohydrates in photosynthesis regulation and the elements triggering the down-regulation process. in this work, the role of non-structural carbohydrates in the regulation of photosynthesis under a broad spectrum of source-sink relationships has been i ...	2011	21367744
analysis of bioactive compounds in seven citrus cultivars.	chemical content in peels of fruits belonging to cultivars of clementine mandarin (fino, loretina and marisol), satsume mandarin (owari), navel orange (navelate and navelina) and common orange (valencia late) groups have been analyzed. the influence of the variety on the content of bioactive constituents at the varietal level is discussed. rind contents showed similar tendencies for the majority of compounds although satsume group presented the highest amounts on the flavanone glycosides hesperi ...	2011	21364046
an expedient and versatile protocol for extracting high-quality dna from plant leaves.	introductionprotocols for extracting plant genomic dna have to contend with two major challenges: preventing the oxidation of phenolic substances that can react with nucleic acids and proteins, and eliminating polysaccharides that interfere with downstream enzymatic manipulations of dna. a protocol has been developed in which the above-mentioned substances are removed from the sample before the dna is removed from the chromatin structure. this protocol requires only two tubes during extraction a ...	2007	21357104
expression analysis in response to low temperature stress in blood oranges: implication of the flavonoid biosynthetic pathway.	the productivity and the geographical distribution of most commercially important citrus varieties are markedly affected by environmental low temperatures. as gene engineering has been shown to be a favourable alternative to produce germplasm with improved cold tolerance, a broad group of cold regulated genes have been previously identified from several citrus spp. by contrast, little information regarding the cold stress response of pigmented sweet orange varieties is available although they mi ...	2011	21349317
citrus tristeza virus infection induces the accumulation of viral small rnas (21-24-nt) mapping preferentially at the 3'-terminal region of the genomic rna and affects the host small rna profile.	to get an insight into the host rna silencing defense induced by citrus tristeza virus (ctv) and into the counter defensive reaction mediated by its three silencing suppressors (p25, p20 and p23), we have examined by deep sequencing (solexa-illumina) the small rnas (srnas) in three virus-host combinations. our data show that ctv srnas: (i) represent more than 50% of the total srnas in mexican lime and sweet orange (where ctv reaches relatively high titers), but only 3.5% in sour orange (where th ...	2011	21327514
antioxidant capacity of hesperidin from citrus peel using electron spin resonance and cytotoxic activity against human carcinoma cell lines.	hesperidin is a flavonoid that has various pharmacological activities including anti-inflammatory, antimicrobial and antiviral activities.	2011	21323480
selection and validation of reference genes for gene expression studies by reverse transcription quantitative pcr in xanthomonas citri subsp. citri during infection of citrus sinensis.	xanthomonas citri subsp. citri (xcc) causes citrus canker, a worldwide disease found mainly in sweet oranges (citrus sinensis (l.) osbeck). the expression of nine candidate internal reference genes was analyzed in xcc grown alone and during c. sinensis infection to identify genes most suitable for comparative expression studies in xcc using reverse transcription quantitative pcr (qrt-pcr). the stability of these genes was determined using the programs genorm, normfinder and bestkeeper. the genes ...	2011	21318633
biomimetic preparation of polymer-supported free radical scavenging, cytocompatible and antimicrobial "green" silver nanoparticles using aqueous extract of citrus sinensis peel.	in the pursuit of making the nanoscale-research greener, the utilization of the reductive potency of a common byproduct of food processing industry i.e. orange peel is reported here to prepare biopolymer-templated "green" silver nanoparticles. aqueous extract of orange peel at basic ph was exploited to prepare starch supported nanoparticles under ambient conditions. the compositional abundance of pectins, flavonoids, ascorbic acid, sugars, carotenoids and myriad other flavones may be envisaged f ...	2011	21316933
preliminary in vitro antisickilng properties of crude juice extracts of persia americana, citrus sinensis, carica papaya and ciklavit®.	the antisickling properties of crude juice extracts of the edible portions of three commonly consumed tropical fruits namely persia americana, citrus sinensis, and carica papaya were investigated in vitro alongside a new drug preparation called ciklavit® that has antisickling activity. four different solvent extracts of the crude juice of each fruit including aqueous, acidic, alkaline and alcoholic extracts were prepared and their antisickling effects on sickle cell trait (hbas) and sickle cell ...	2009	21304622
[research on zinc content in leaf of olinda valencia orange using visible near infrared spectroscopy model].	olinda valencia orange leaves dry powder-like were taken as sample, and chemical analysis combined with technology of visible near-infrared spectroscopy (vis/nirs) was used, through the treatment process of second derivative spectrum of samples of the original spectrum and denoising (noise). meanwhile, method of partial least squares (pls) and cross-validation were used to establish maths model of zn concentration which applying band combination composited by 400-500 and 1201-1300 nm of characte ...	2010	21284155
azteca barbifex forel (hymenoptera: formicidae): potential pest of citrus crops in eastern amazon.	damage caused by the ant azteca barbifex (forel) was identified in orange trees (citrus sinensis), in capitão poço county, guamá microregion, pará state. the damage caused by the scraping of stems and branches lead to reduction in yield with subsequent death of the plant. these characteristics indicate a. barbifex as a potential pest of citrus crops in the eastern region of amazon.	2010	21271082
distribution of aroma volatile compounds in tangerine hybrids and proposed inheritance.	with the desirable combination of sugars and acids, volatile compounds contribute to the essential organoleptic attributes of citrus. this study evaluated the aroma volatiles of 20 tangerine hybrids of the university of florida breeding program. volatiles were sampled from hand-squeezed juice by headspace solid-phase microextraction (spme), and analyzed by gas chromatography-mass spectrometry. principal component analysis (pca) and cluster analysis (ca) were used to find similarities among sampl ...	2010	21218478
insecticidal activity against bemisia tabaci biotype b of peel essential oil of citrus sinensis var. pear and citrus aurantium cultivated in northeast brazil.	the fumigant action of peel essential oils of citrus sinensis var. pear (pear orange = po) and c. aurantium (bitter orange = bo) from the northeast of brazil were evaluated against bemisia tabaci biotype b and compared with eugenol as a positive control. the oil concentration in the po at 8.5 microl/l of air caused 97% mortality, while the oil concentration of bo at 9.5 microl/l of air caused 99% mortality. however, the lc50 estimates for both oils (lc50 = 3.80 microl/l of air for po and lc50 = ...	2010	21213990
mosquito larvicidal activity of citrus limonoids against aedes albopictus.	citrus limonoids, nomilin and limonin, were used for larvicidal assay against aedes albopictus utilizing who methodology. lc(50s) were 305.83, 176.08, and 136.07 μm for nomilin and 850.09, 600.72, and 407.09 μm for limonin after 24, 48, and 72 h, respectively. lt(50) assays exhibited that savage citrange oil was the best at all concentrations (400, 500, 600, and 700 ppm) while fairchild and cassa grande were the weakest oils at 400 ppm, but at 500, 600, and 700 ppm, carrizo citrange remained at ...	2011	21212981
elsinoë fawcettii and elsinoë australis: the fungal pathogens causing citrus scab.	elsinoë fawcettii and e. australis are important pathogens of citrus. both species are known to produce red or orange pigments, called elsinochrome. elsinochrome is a nonhost-selective phytotoxin and is required for full fungal virulence and lesion formation. this article discusses the taxonomy, epidemiology, genetics and pathology of the pathogens. it also provides a perspective on the cellular toxicity, biosynthetic regulation and pathological role of elsinochrome phytotoxin. taxonomy: elsinoë ...	2010	21199563
rapid simultaneous determination of amines and organic acids in citrus using high-performance liquid chromatography.	rapid analytical method for the simultaneous separation and determination of amines and organic acids is a vital interest for quality control of citrus and their products. in the present study, a simultaneous high performance liquid chromatography (hplc) method for the rapid separation of three amines and two organic acids was developed. chromatographic separation of compounds was achieved using xbridge c(18) column at ambient temperature, with an isocratic mobile phase of 3mm phosphoric acid at ...	2010	21147342
stage and tissue-specific modulation of ten conserved mirnas and their targets during somatic embryogenesis of valencia sweet orange.	somatic embryogenesis (se) is a remarkable process of plant somatic cells developing into an embryo capable of forming a complete plant. mirnas play important roles in plant development by regulating expression of their target genes, but its function in se has rarely been studied. herein, ten conserved mirnas with critical functions in plant development are detected by stem-loop qrt-pcr in the se system of valencia sweet orange. sixteen unigenes from citrus are predicted to be targeted by six of ...	2011	21103993
resistance to citrus psorosis virus in transgenic sweet orange plants is triggered by coat protein-rna silencing.	the lack of naturally occurring resistance to citrus psorosis virus (cpsv) has demanded exploitation of a transgenic approach for the development of cpsv-resistant sweet orange plants. transgenic sweet orange plants producing intron-hairpin rna transcripts (ihprna) corresponding to viral cp, 54k or 24k genes were generated and analyzed at the molecular and phenotypic levels. two independent cpsv challenge assays demonstrated that expression of ihprna derived from the cp gene (ihpcp) provided a h ...	2010	21084056
antiplasmodial activity of botanical extracts against plasmodium falciparum.	the absence of a vaccine and the rampant resistance to almost all antimalarial drugs have accentuated the urgent need for new antimalarial drugs and drug targets for both prophylaxis and chemotherapy. the aim of the study was to discover effective plant extracts against plasmodium falciparum. in the present study, the hexane, chloroform, ethyl acetate, acetone, and methanol extracts of citrus sinensis (peel), leucas aspera, ocimum sanctum, phyllanthus acidus (leaf), terminalia chebula (seed) wer ...	2010	21079994
effective sampling range of a synthetic protein-based attractant for ceratitis capitata (diptera: tephritidae).	studies were conducted in honduras to determine effective sampling range of a female-targeted protein-based synthetic attractant for the mediterranean fruit fly, ceratitis capitata (wiedemann) (diptera: tephritidae). multilure traps were baited with ammonium acetate, putrescine, and trimethylamine lures (three-component attractant) and sampled over eight consecutive weeks. field design consisted of 38 traps (over 0.5 ha) placed in a combination of standard and high-density grids to facilitate ge ...	2010	21061993
identification of thermolabile pectin methylesterases from sweet orange fruit by peptide mass fingerprinting.	the multiple forms of the enzyme pectin methylesterase (pme) present in citrus fruit tissues vary in activity toward juice cloud-associated pectin substrates and, thus, in their impact on juice cloud stability and product quality. because the proteins responsible for individual pme activities are rarely identified by structural properties or correlated to specific pme genes, matrix-assisted laser desorption-ionization with tandem time-of-flight mass spectrometry (maldi-tof/tof ms) was investigat ...	2010	21053908
gc-ms analysis of headspace and liquid extracts for metabolomic differentiation of citrus huanglongbing and zinc deficiency in leaves of 'valencia' sweet orange from commercial groves.	citrus huanglongbing (hlb) is considered the most destructive citrus disease worldwide. symptoms-based detection of hlb is difficult due to similarities with zinc deficiency.	2015	21046688
transformation of sweet orange [citrus sinensis (l.) osbeck] with ptha-nls for acquiring resistance to citrus canker disease.	the cooh terminal of ptha encoding three nuclear localizing signals (nls) was amplified by polymerase chain reaction (pcr) from the plasmid of xanthomonas axonopodis pv. citri, the pathogen of citrus canker disease. then the sense and antisense strands of the nls were cloned into pbi121 vector. ptha-nls driven by the camv35 s promoter was transferred into sweet orange via agrobacterium -mediated transformation. successful integration was confirmed by pcr and southern blotting, and 12 sense-nls ( ...	2010	20972821
characterization of three terpenoid glycosyltransferase genes in 'valencia' sweet orange (citrus sinensis l. osbeck).	three putative terpenoid udp-glycosyltransferase (ugt) genes, designated csugt1, csugt2, and csugt3, were isolated and characterized in 'valencia' sweet orange (citrus sinensis l. osbeck). csugt1 consisted of 1493 nucleotides with an open reading frame encoding 492 amino acids, csugt2 consisted of 1727 nucleotides encoding 504 amino acids, and csugt3 consisted of 1705 nucleotides encoding 468 amino acids. csugt3 had a 145 bp intron at 730-874, whereas csugt1 and csugt2 had none. the three deduce ...	2010	20962888
effects of pymetrozine, an antifeedant of hemiptera, on asian citrus psyllid, diaphorina citri, feeding behavior, survival and transmission of candidatus liberibacter asiaticus.	vector-borne plant disease management can be enhanced by deployment of antifeedants in addition to the use of broad-spectrum neurotoxic insecticides. the effects of pymetrozine on asian citrus psyllid (acp), diaphorina citri kuwayama, feeding behaviour, survival and transmission of candidatus liberibacter asiaticus (las), the presumed causal pathogen of huanglongbing, were investigated.	2010	20960465
effect of chlorine dioxide gas on salmonella enterica inoculated on navel orange surfaces and its impact on the quality attributes of treated oranges.	microorganisms, including pathogens of public health significance, have been shown to contaminate orange juice during the mechanical extraction of juice. the problem gets exacerbated when washed oranges have high initial microbial load, due to an insufficient postharvest treatment. the objective of this study was to investigate the reduction of salmonella enterica on orange surfaces using clo₂ gas treatments to achieve a 5 log reduction, consistent with the recommendations of the u.s. department ...	2010	20932090
comprehensive analysis of expressed sequence tags from the pulp of the red mutant 'cara cara' navel orange (citrus sinensis osbeck).	expressed sequence tag (est) analysis of the pulp of the red-fleshed mutant 'cara cara' navel orange provided a starting point for gene discovery and transcriptome survey during citrus fruit maturation. interpretation of the est datasets revealed that the mutant pulp transcriptome held a high section of stress responses related genes, such as the type iii metallothionein-like gene (6.0%), heat shock protein (2.8%), cu/zn superoxide dismutase (0.8%), late embryogenesis abundant protein 5 (0.8%), ...	2010	20883438
citrus fruit extracts reduce advanced glycation end products (ages)- and h(2)o(2)-induced oxidative stress in human adipocytes.	diabetes is a reactive oxygen species (ros)-mediated pathology, with a worldwide prevalence estimated to double by 2030. a major effort has been launched to find therapeutic means to improve health conditions of diabetic patients. recent data show that supplemental natural antioxidants represent a potential strategy as adjunct therapy. despite the major role of adipocytes in the etiology of diabetes, little is known about the effect of natural antioxidants on adipocyte response to oxidative stre ...	2010	20882960
an attempt of postharvest orange fruit rot control using essential oils from mediterranean plants.	twelve essential oils from mediterranean aromatic plants were tested at different doses against four fungi known as causal agents of post-harvest orange fruit rot: botrytis cinerea, penicillium italicum, phytophthora citrophthora, and rhizopus stolonifer. essential oils were obtained from hyssopus officinalis, lavandula angustifolia, majorana hortensis, melissa officinalis, ocimum basilicum, origanum vulgare, salvia officinalis, and thymus vulgaris (family lamiaceae), verbena officinalis (family ...	2010	20874226
juice components and antioxidant capacity of four tunisian citrus varieties.	juices from four citrus species of tunisia were investigated mainly for quality parameters and antioxidant capacity.	2011	20862741
involvement of inducible nitric oxide synthase and cyclooxygenase-2 in the anti-inflammatory effects of a red orange extract in human chondrocytes.	in the present study, a complex of compounds (red orange complex, roc), obtained from three red orange varieties (citrus sinensis varieties: moro, tarocco and sanguinello), containing cyanidin glycosides, hydroxycinnamic acids, flavanone glycosides and ascorbic acid, was screened to discover new lead compounds in the suppression of the production of key molecules released during inflammatory events in interleukin-1beta (il-beta) stimulated human primary chondrocytes. the expression of inducible ...	2010	20812134
shedding light on the role of photosynthesis in pathogen colonization and host defense.	the role of photosynthesis in plant defense is a fundamental question awaiting further molecular and physiological elucidation. to this end we investigated host responses to infection with the bacterial pathogen xanthomonas axonopodis pv. citri, the pathogen responsible for citrus canker. this pathogen encodes a plant-like natriuretic peptide (xacpnp) that is expressed specifically during the infection process and prevents deterioration of the physiological condition of the infected tissue. prot ...	2010	20798833
effect of ploidy increase on transgene expression: example from citrus diploid cybrid and allotetraploid somatic hybrid expressing the egfp gene.	polyploidization is an important speciation mechanism for all eukaryotes, and it has profound impacts on biodiversity dynamics and ecosystem functioning. green fluorescent protein (gfp) has been used as an effective marker to visually screen somatic hybrids at an early stage in protoplast fusion. we have previously reported that the intensity of gfp fluorescence of regenerated embryoids was also an early indicator of ploidy level. however, little is known concerning the effects of ploidy increas ...	2010	20734092
synergistic and antagonistic interactions of phenolic compounds found in navel oranges.	phenolic compounds are known to have antioxidant and antimicrobial properties. these properties may be useful in the preservation of foods or beverages. the interactive antioxidant capacity of phenolic compounds within foods has not been well explored. interactions of individual phenolic compounds (chlorogenic acid, hesperidin, luteolin, myricetin, naringenin, p-coumaric acid, and quercetin) at the concentrations found in navel oranges (citrus sinensis) were analyzed for their antioxidant capaci ...	2010	20722912
[application of aas to detecting the influence of railway on the soil of navel orange garden].	railway transportation has boosted the economy of railway road area, meanwhile it brings some undesirable impacts on the environment of the railway road area. the quality of the fruits is directly related with the elements of the soil, so understanding the element contents of soil in navel oranges garden in the vicinity of railway is meaningful to the security of agriculture products and ecological conditions in the areas surrounding the railways. as a favorite fruit, navel orange is widely plan ...	2010	20707171
chemical constituents of citrus sinensis var. shukri from pakistan.	the variety 'shukri' is a new hybrid of citrus sinensis and is frequently grown for its sweet edible fruits in the southern part of pakistan. the leaves of this hybrid variety have been investigated in search of secondary metabolites for the first time. as a result of chromatographic analysis of the methanolic extract, a new ceramide along with a flavonone glycoside and two steroids has been isolated, which were spectroscopically characterized as (e)-n-(1,3,4,5-tetrahydroxyhexadecan-2-yl)dec-4-e ...	2010	20706908
early events of citrus greening (huanglongbing) disease development at the ultrastructural level.	citrus greening (huanglongbing [hlb]) is one of the most destructive diseases of citrus worldwide. the causal agent of hlb in florida is thought to be 'candidatus liberibacter asiaticus'. understanding of the early events in hlb infection is critical for the development of effective measures to control the disease. in this work, we conducted cytopathological studies by following the development of the disease in citrus trees graft inoculated with 'ca. l. asiaticus'-containing material under gree ...	2010	20701493
evaluation of in vitro activity of essential oils against trypanosoma brucei brucei and trypanosoma evansi.	essential oils (eos) from cymbopogon citratus (cc), eucalyptus citriodora (ec), eucalyptus camaldulensis (ed), and citrus sinensis (cs) were obtained by hydrodistillation process. the eos were evaluated in vitro for activity against trypanosoma brucei brucei (tbb) and trypanosoma evansi (t. evansi). the eos were found to possess antitrypanosomal activity in vitro in a dose-dependent pattern in a short period of time. the drop in number of parasite over time was achieved doses of 0.4 g/ml, 0.2 g/ ...	2010	20700425
timing of the inhibitory effect of fruit on return bloom of 'valencia' sweet orange (citrus sinensis (l.) osbeck).	in citrus the inhibitory effect of fruit on flower formation is the main cause of alternate bearing. although there are some studies reporting the effect on flowering of the time of fruit removal in a well-defined stage of fruit development, few have investigated the effect throughout the entire fruit growth stage from early fruitlet growth to fruit maturity. the objective of this study was to determine the phenological fruit developmental stage at which the fruit begins its inhibitory effect on ...	2010	20564309
comparative morpho-anatomical studies of the lesions caused by citrus leprosis virus on sweet orange.	the leprosis disease shows a viral etiology and the citrus leprosis virus is considered its etiologic agent. the disease may show two types of cytopatologic symptom caused by two virus: nuclear (cilv-n) and cytoplasmic (cilv-c) types. the aim of this study was to compare the morpho-anatomical differences in the lesions caused by leprosis virus-cytoplasmic and nuclear types in citrus sinensis (l.) osbeck 'pêra'. leaf and fruit lesions were collected in piracicaba/são paulo (cytoplasmic type) and ...	2010	20563430
physicochemical and molecular analysis of cell wall metabolism between two navel oranges (citrus sinensis) with different mastication traits.	fj72-1 navel orange and its bud mutant fjwc exhibit differences in melting texture character which are influenced mostly by cell wall metabolism. here we compared the contents of water soluble pectin (wsp), protopectin, total pectin (tp), cellulose, and hemicellulose, activities of polygalaturonase (pg), pectin methylesterase (pme), pectate lyases (pl), cellulase (cel) and gene expression levels of pg, pme, pl and cel between the two cultivars.	2010	20549800
effect of liberibacter infection (huanglongbing or "greening" disease) of citrus on orange juice flavor quality by sensory evaluation.	some anecdotal reports suggest that infection of citrus trees with candidatus liberibacter asiaticus (las), the suspected causal agent of huanglongbing (hlb) disease, imparts off flavor to orange juice. it is of interest to the industry to know how las infection affects juice quality with respect to cultivar, maturity, or processing method. hamlin, midsweet, and valencia oranges were harvested over 2 y from trees that tested negative (las-) or positive (las+) for las from different groves and in ...	2010	20546425
[study on synchronous correlation between fruit characteristic spectrum and the parameter of internal quality for hamlin sweet orange fruit].	the purpose of our study is to study the relationship between the reflective spectrum of fruit and the internal quality of the orange fruit and find the suitable mark spectrum that can synchronously measure the several fruit quality index at same time to lay a foundation for the development of a rapid and nondestructive field fruit quality analysis technique by analyzing the visible-near infrared spectrum. mature hamlin orange (citrus sinensis (l)cv. hamlin sweet orange) fruits were individually ...	2010	20545159
glutathione peroxidase regulation of reactive oxygen species level is crucial for in vitro plant differentiation.	phospholipid hydroperoxide glutathione peroxidase (phgpx) is overexpressed in plants under abiotic and biotic stress conditions that mediate oxidative stress. to study its biological role and its ability to confer stress resistance in plants, we tried to obtain transgenic plants overexpressing citrus (citrus sinensis) phgpx (cit-phgpx). all attempts to obtain regenerated plants expressing this enzyme constitutively failed. however, when the enzyme's catalytic activity was abolished by active sit ...	2010	20530511
proteome from lemon fruit flavedo reveals that this tissue produces high amounts of the cit s1 germin-like isoforms.	a multistep procedure has been developed and applied to extract and purify proteins from lemon fruit flavedo. 2de, lc-esi-ms/ms, and bioinformatics were used to detect the high abundance of the germin-like glycoprotein cit s1, a powerful allergen in humans. peptide alignments against citrus est repositories gave the best scores with the c. sinensis cdna (gi\|188354270/ey710037), annotated as unknown sweet orange fruit protein; additional blast of peptides against ncbi databases gave high sequence ...	2010	20499937
chemical characterization of orange juice from trees infected with citrus greening (huanglongbing).	the effects due to candidatus liberibacter infection, commonly called citrus greening or huanglongbing (hlb), on volatile and nonvolatile components of orange juices, oj, were examined using gc-ms and high-performance liquid chromatography (hplc). hlb symptomatic, asymptomatic, and control "hamlin" and "valencia" oranges were harvested from december to may during the 2007 to 2008 harvest season. brix/acid levels in control and asymptomatic juices were similar but symptomatic juices were as much ...	2010	20492226
stereoselective conjugation, transport and bioactivity of s- and r-hesperetin enantiomers in vitro.	the flavanone hesperetin ((+/-)-4'-methoxy-3',5,7-trihydroxyflavanone) is the aglycone of hesperidin, which is the major flavonoid present in sweet oranges. hesperetin contains a chiral c-atom and so can exist as an s- and r-enantiomer, however, in nature 2s-hesperidin and its s-hesperetin aglycone are predominant. the present study reports a chiral hplc method to separate s- and r-hesperetin on an analytical and semipreparative scale. this allowed characterization of the stereoselective differe ...	2010	20441166
acaricidal activity against tetranychus urticae and chemical composition of peel essential oils of three citrus species cultivated in ne brazil.	the repellency and fumigant toxicities of the peel essential oils of citrus sinensis var. pêra (lp), c. sinensis var. mimo (lm), and c. aurantium (ll) cultivated in northeast brazil were evaluated against tetranychus urticae. analysis of the oils by gc and gc/ms led to the identification of twenty-eight components, which represented 99.9%, 99.7% and 99.3% of the total constituents of the lp, lm and ll oils, respectively. limonene was the main component found in all three oils. other main compone ...	2010	20420330
free and bound cinnamic acid derivatives in corsica sweet blond oranges.	total determination of cinnamic acids (ca), including hydroxycinnamic acid derivatives is generally not accurate since, during hydrolysis, a possible degradation of dihydroxy ca such as caffeic acid could occur. evaluations of ca (ferulic, p-coumaric, sinapic, cinnamic and caffeic acids) before and after hydrolysis have been undertaken using standards and either with or without addition of ascorbic acid and edta. the method was then applied to the determination of free and bound ca in five blond ...	2010	20420324
discovery and comparative profiling of micrornas in a sweet orange red-flesh mutant and its wild type.	red-flesh fruit is absent from common sweet orange varieties, but is more preferred by consumers due to its visual attraction and nutritional properties. our previous researches on a spontaneous red-flesh mutant revealed that the trait is caused by lycopene accumulation and is regulated by both transcriptional and post-transcriptional mechanisms. however, the knowledge on post-transcriptional regulation of lycopene accumulation in fruits is rather limited so far.	2010	20398412
light controls phospholipase a2alpha and beta gene expression in citrus sinensis.	the low-molecular weight secretory phospholipase a2alpha (csspla2alpha) and beta (cspla2beta) cloned in this study exhibited diurnal rhythmicity in leaf tissue of citrus sinensis. only csspla2alpha displayed distinct diurnal patterns in fruit tissues. csspla2alpha and cspla2beta diurnal expression exhibited periods of approximately 24 h; csspla2alpha amplitude averaged 990-fold in the leaf blades from field-grown trees, whereas cspla2beta amplitude averaged 6.4-fold. diurnal oscillation of csspl ...	2010	20388744
the effects of host, geographic origin, and gender on the thermal requirements of diaphorina citri (hemiptera: psyllidae).	diaphorina citri kuwayama (hemiptera: psyllidae) is the vector of the bacteria that causes citrus greening and is considered one of the world's most important citrus diseases. we examined how host, geographic region, and gender affect the thermal requirements of d. citri. the insects were reared in climatic chambers at constant temperatures of 18, 20, 22, 25, 28, 30, and 32 +/- 1 degrees c, 70 +/- 10% rh, and a 14 h photophase. host plants for d. citri included orange (citrus sinensis [rutaceae] ...	2010	20388302