Publications

physical location of the carotenoid biosynthesis genes psy and β-lcy in capsicum annuum (solanaceae) using heterologous probes from citrus sinensis (rutaceae).	carotenoids are responsible for a range of fruit colors in different hot pepper (capsicum) varieties, from white to deep red. color traits are genetically determined by three loci, y, c1, and c2, which are associated with carotenogenic genes. although such genes have been localized on genetic maps of capsicum and anchored in lycopersicon and solanum, physical mapping in capsicum has been restricted to only a few clusters of some multiple copy genes. heterologous probes from single copy genes hav ...	2011	21425090
ectopic expression of mdspds1 in sweet orange (citrus sinensis osbeck) reduces canker susceptibility: involvement of hôééoôéé production and transcriptional alteration.	enormous work has shown that polyamines are involved in a variety of physiological processes, but information is scarce on the potential of modifying disease response through genetic transformation of a polyamine biosynthetic gene.	2011	21439092
determination of volatile, phenolic, organic acid and sugar components in a turkish cv. dortyol (citrus sinensis l. osbeck) orange juice.	orange flavour is the results of a natural combination of volatile compounds in a well-balanced system including sugars, acids and phenolic compounds. this paper reports the results of the first determination of aroma, organic acids, sugars, and phenolic components in dortyol yerli orange juices.	2011	21480267
inhibition of tnf-α/ifn-γ induced rantes expression in hacat cell by naringin.	context: naringin is a bioflavonoid derivative and is predominantly found in citrus paradisi macf., citrus sinensis (linn.) osbeck, citrus unshiu marc., citrus reticulata blanco cv. nobilis, citrus tachibana (makino) tanaka, citrus junos sieb. ex tanaka (rutaceae), and related citrus species. it has anti-inflammatory effects that have been well-documented, but the mechanism is poorly characterized. objective: the effect of naringin on production of rantes (regulated upon activation normal t-cell ...	2011	21500970
host status of grapefruit and valencia oranges for anastrepha serpentina and anastrepha ludens (diptera: tephritidae).	anastrepha serpentina (wiedemann) (diptera: tephritidae) is sporadically captured in the rio grande valley of texas. although its preferred hosts are in the sapotaceae family, several varieties of citrus, including grapefruit and oranges are listed as alternate hosts. although mexican fruit fly, anastrepha ludens (loew), is known to be a major pest of citrus, doubt exists as to the status of citrus as a breeding host for a. serpentina. to evaluate the host status of commercial citrus for a. serp ...	2011	21510184
terpene down-regulation in orange reveals the role of fruit aromas in mediating interactions with insect herbivores and pathogens.	plants use volatile terpene compounds as odor cues for communicating with the environment. fleshy fruits are particularly rich in volatiles that deter herbivores and attract seed dispersal agents. we have investigated how terpenes in citrus fruit peels affect the interaction between the plant, insects, and microorganisms. because limonene represents up to 97% of the total volatiles in orange (citrus sinensis) fruit peel, we chose to down-regulate the expression of a limonene synthase gene in ora ...	2011	21525333
biosynthesis of silver nanoparticles using citrus sinensis peel extract and its antibacterial activity.	biosynthesis of silver nanoparticles (agnps) was achieved by a novel, simple green chemistry procedure using citrus sinensis peel extract as a reducing and a capping agent. the effect of temperature on the synthesis of silver nanoparticles was carried out at room temperature (25°c) and 60°c. the successful formation of silver nanoparticles has been confirmed by uv-vis, ftir, xrd, edax, fesem and tem analysis and their antibacterial activity against escherichia coli, pseudomonas aeruginosa (gram- ...	2011	21536485
partial dehydration and cryopreservation of citrus seeds.	three categories of seed storage behavior are generally recognized among plant species: orthodox, intermediate and recalcitrant. intermediate seeds cannot be stored in liquid nitrogen (ln) without a previous partial dehydration process. the water content (wc) of the seeds at the moment of immersion in ln must be regarded as the most critical factor in cryopreservation. the purpose of this study was to investigate the basis of the optimal hydration status for cryopreservation of citrus seeds: c. ...	2011	21538368
tetraploidization events by chromosome doubling of nucellar cells are frequent in apomictic citrus and are dependent on genotype and environment.	background and aims polyploidy is a major component of plant evolution. the citrus gene pool is essentially diploid but tetraploid plants are frequently encountered in seedlings of diploid apomictic genotypes. the main objectives of the present study were to establish the origin of these tetraploid plants and to ascertain the importance of genotypic and environmental factors on tetraploid formation. methods tetraploid seedlings from 30 diploid apomictic genotypes were selected by flow cytometry ...	2011	21586529
the sucrose synthase-1 promoter from citrus sinensis directs expression of the β-glucuronidase reporter gene in phloem tissue and in response to wounding in transgenic plants.	interest in phloem-specific promoters for the engineering of transgenic plants has been increasing in recent years. in this study we isolated two similar, but distinct, alleles of the citrus sinensis sucrose synthase-1 promoter (cssus1p) and inserted them upstream of the β-glucuronidase (gus) gene to test their ability to drive expression in the phloem of transgenic arabidopsis thaliana and nicotiana tabacum. although both promoter variants were capable of conferring localized gus expression in ...	2011	21594624
distribution and biological role of the oligopeptide-binding protein (oppa) in xanthomonas species.	in this study we investigated the prevalence of the oppa gene, encoding the oligopeptide binding protein (oppa) of the major bacterial oligopeptide uptake system (opp), in different species of the genus xanthomonas. the oppa gene was detected in two xanthomonas axonopodis strains among eight tested xanthomonas species. the generation of an isogenic oppa-knockout derivative of the xac 306 strain, showed that the oppa protein neither plays a relevant role in oligopeptide uptake nor contributes to ...	2010	21637492
limonoid content of sour orange varieties.	modern citrus cultivars are thought to have arisen from three parents: the pummelo, the mandarin, and citron. taxological and genetic data support that sweet and sour oranges share a common parentage. however, as their name suggests, the organoleptic properties of the fruit from these two families is distinctly different. analysis of the limonoid content of sour orange varieties has been limited.	2011	21681758
colonization of citrus seed coats by 'candidatus liberibacter asiaticus': implications for seed transmission of the bacterium.	huanglongbing is an economically damaging disease of citrus associated with infection by 'ca. liberibacter asiaticus'. transmission of the organism via infection of seeds has not been demonstrated but is a concern since some citrus varieties, particularly those used as rootstocks in commercial plantings are propagated from seed. we compared the incidence of detection of 'ca. liberibacter asiaticus' dna in individual fruit peduncles, seed coats, seeds and in germinated seedlings from 'sanguenelli ...	2011	21714779
ectopic expression of the p23 silencing suppressor of citrus tristeza virus differentially modifies viral accumulation and tropism in two transgenic woody hosts.	citrus tristeza virus (ctv), a phloem-restricted closterovirus infecting citrus, encodes three different silencing suppressors (p25, p20 and p23), one of which (p23) is a pathogenicity determinant that induces aberrations resembling ctv symptoms when expressed ectopically in transgenic citrus hosts. in this article, the effect of p23 ectopic expression on virus infection was examined in sweet orange (swo), a highly susceptible host, and sour orange (so), which severely restricts ctv cell-to-cell ...	2011	21726389
fiber concentrate from orange (citrus sinensis l.) bagase: characterization and application as bakery product ingredient.	orange is a tropical fruit used in the juice industry, yielding important quantities of by products. the objective of this work was to obtain a dietary fiber-rich orange bagasse product (dfrobp), evaluate its chemical composition and its use in the preparation of a bakery product (muffin). muffins containing two different levels of dfrobp were studied regarding chemical composition, in vitro starch digestibility, predicted glyceamic index and acceptability in a sensory test. dfrobp showed low fa ...	2011	21731434
isolation, identification and sequence analysis of a thioredoxin h gene, a member of subgroup iii of h-type trxs from grape (vitis vinifera l. cv. askari).	thioredoxins (trxs) are small ubiquitous proteins which play a regulatory role in a variety of cellular processes. in contrast to other organisms, plants have a great number of trx types, consisting of six well-defined groups: f, m, x, and y in chloroplasts, o in mitochondria, and h mainly in cytosol. a full-length cdna, designated vvcxxs2, encoding trx h polypeptide was isolated and cloned from grape (vitis vinifera l. cv. askari) berries organ by reverse transcription polymerase chain reaction ...	2011	21732058
comparison of cell wall metabolism in the pulp of three cultivars of 'nanfeng' tangerine differing in mastication trait.	background: like sweet orange (citrus sinensis), tangerine (citrus reticulata) is another citrus crop grown widely throughout the world. however, whether it shares a common mechanism with sweet orange in forming a given mastication trait is still unclear. in this study, three 'nanfeng' tangerine cultivars, 'yangxiao-26' ('yx-26') with inferior mastication trait, elite 'yx-26' with moderate mastication trait and 'miguang' ('mg') with superior mastication trait, were selected to investigate the fo ...	2011	21732384
chemical elements in organic and conventional sweet oranges.	this work focuses on the determination of chemical elements in sweet oranges of variety valencia produced under organic and conventional systems using instrumental neutron activation analysis and inductively coupled plasma mass spectrometry. the distribution of chemical elements was variable among the fruit parts with usually higher concentrations of br, ca, ce, k, la, na, rb, and sc in the peel. however, k, na, and rb also presented high values in the juice samples, while fe and zn were higher ...	2011	21735113
to compare the efficacy of two kinds of zhizhu pills in the treatment of functional dyspepsia of spleen-deficiency and qi-stagnation syndrome: a randomized group sequential comparative trial.	in traditional chinese medicine (tcm) theory, functional dyspepsia (fd) can be divided into different syndromes according to different clinical symptoms and signs, and the most common one is spleen-deficiency and qi-stagnation syndrome that can be treated by chinese traditional patent medicine--two kinds of zhizhu pills, between which the primary difference in ingredients is that one contains immature orange fruit of citrus aurantium l.(ifca) and the other contains that of citrus sinensis osbeck ...	2011	21762493
[research on optimization of model for detecting sugar content of navel orange by online near infrared spectroscopy].	the objective of the present research was to optimize the model of sugar content in navel orange for improving the detection presicion by the online near infrared spectroscopy. the reference wavelength was chosen by coefficient of variation of the different wavelengths in the calibration set in the wavelength range of 700.28 - 933.79 nm. then the spectra were transformed into ratio specra. the absorbance and ration spectra were pretreated by different preprocessing methods. the models of sugar c ...	2011	21800571
cloning, expression, purification and physical and kinetic characterization of the phosphoenolpyruvate carboxylase from orange (citrus sinensis osbeck var. valencia) fruit juice sacs.	phosphoenolpyruvate (pep) carboxylase (pepcase) from orange fruit juice sacs has been cloned and heterogously expressed in high yield. the purified recombinant enzyme displays properties typical of plant pepcase, including activation by sugar phosphates and inhibition by malate and citrate. malate inhibition is weak in the physiological ph range, and the enzyme is also poorly affected by glu and asp, known inhibitors of c(3) plants pepcases. however, it is strongly inhibited by citrate. orange f ...	2010	21802611
citrus essential oil of nigeria. part v: volatile constituents of sweet orange leaf oil (citrus sinensis).	the volatile oils extracted from leaves of eight cultivars of citrus sinensis (l) osbeck were comprehensively analysed by a combination of gc and gc-ms. fifty four constituents accounting for 82.3-98.2% were identified. sabinene (20.9-49.1%), delta-3-carene (0.3-14.3%), (e)-beta-ocimene (4.4-12.6%), linalool (3.7-11.1%) and terpinen-4-ol (1.7-12.5%) were the major constituents that are common to all the volatile oils. in addition, a cluster analysis was carried out and indicated at least four di ...	2011	21815430
comparative analysis of the volatile fraction of fruit juice from different citrus species.	the volatile composition of fruit from four citrus varieties (powell navel orange, clemenules mandarine, and fortune mandarine and chandler pummelo) covering four different species has been studied. over one hundred compounds were profiled after hs-spme-gc-ms analysis, including 27 esters, 23 aldehydes, 21 alcohols, 13 monoterpene hydrocarbons, 10 ketones, 5 sesquiterpene hydrocarbons, 4 monoterpene cyclic ethers, 4 furans, and 2 aromatic hydrocarbons, which were all confirmed with standards. th ...	2011	21818287
comparative itraq proteome and transcriptome analyses of sweet orange infected by candidatus liberibacter asiaticus.	citrus huanglongbing (hlb) has been threatening citrus production worldwide. in this study, a comparative proteomic approach was applied to understand the pathogenic process of hlb in affected sweet orange leaves. using the isobaric tags for relative and absolute quantification (itraq) technique, we identified 686 unique proteins in the mature leaves of both mock-inoculated and diseased 'madam vinous' sweet orange plants. of the identified proteins, 20 and 10 were differentially expressed in lea ...	2011	21838733
longevity of multiple species of tephritid (diptera) fruit fly parasitoids (hymenoptera: braconidae: opiinae) provided exotic and sympatric-fruit based diets.	while adult parasitic hymenoptera in general feed on floral and extrafloral nectars, hemipteran-honeydews and fluids from punctured hosts, diachasmimorpha longicaudata (ashmead), an old world opiine braconid introduced to tropical/subtropical america for the biological control of anastrepha spp. (tephritidae), can survive on fruit juices as they seep from injured fruit. an ability to exploit fruit juice would allow such a parasitoid to efficiently forage for hosts and food sources simultaneously ...	2011	21839085
controlling for sugar and ascorbic acid, a mixture of flavonoids matching navel oranges significantly increases human postprandial serum antioxidant capacity.	fruit and vegetable consumption reduces the risk for cardiovascular disease development. the postprandial state is an important contributor to chronic disease development. orange flavonoids may reduce postprandial oxidation. it was hypothesized that a mixture of orange flavonoids would reduce postprandial oxidation better than a single orange flavonoid or orange sugar and ascorbic acid, but not as well as orange juice, when consumed with a typical breakfast. a placebo-controlled crossover trial ...	2011	21840468
a proteomic analysis of the chromoplasts isolated from sweet orange fruits [citrus sinensis (l.) osbeck].	here, a comprehensive proteomic analysis of the chromoplasts purified from sweet orange using nycodenz density gradient centrifugation is reported. a gelc-ms/ms shotgun approach was used to identify the proteins of pooled chromoplast samples. a total of 493 proteins were identified from purified chromoplasts, of which 418 are putative plastid proteins based on in silico sequence homology and functional analyses. based on the predicted functions of these identified plastid proteins, a large propo ...	2011	21841170
mutations of β-tubulin codon 198 or 200 indicate thiabendazole resistance among isolates of penicillium digitatum collected from citrus in taiwan.	penicillium digitatum causes green mold on citrus, resulting in severe postharvest fruit decay and economic losses in many citrus-producing areas of the world. forty isolates of p. digitatum were cultured from citrus groves, packinghouses, and local markets in taiwan, and assessed quantitatively for their sensitivity to thiabendazole (tbz) fungicide. sensitivity assays using a 96-well microtiter plate revealed that, of 40 isolates examined, only one isolate collected from fruit produced in taiwa ...	2011	21868117
cloning, purification and characterization of a 90kda heat shock protein from citrus sinensis (sweet orange).	protein misfolding is stimulated by stress, such as heat, and heat shock proteins (hsps) are the first line of defense against these undesirable situations. plants, which are naturally sessile, are perhaps more exposed to stress factors than some other organisms, and consequently, the role of hsps is crucial to maintain homeostasis. hsp90, because of its key role in infection and other stresses, is targeted in therapies that improve plant production by increasing resistance to both biotic and ab ...	2012	21873074
evaluation of effects of zingiber officinale on salivation in rats.	there are some herbal plants in iranian traditional system of medicine which are believed to be excellent remedies to alleviate the symptoms of xerostomia. the aim of the present study was to evaluate the effect of systemic administration of seven different herbal extracts on the rate of salivation in rats. the extracts of 7 herbs; zingiber officinale roscoe (zingiberaceae), citrus sinensis (l.) osbeck (rutaceae), artemisia absinthium l. (asteraceae), cichorium intybus l. (asteraceae), pimpinell ...	2011	21874635
cold treatment of ceratitis capitata (diptera: tephritidae) in oranges using a larval endpoint.	south africa currently exports fresh citrus (citrus spp.) fruit to japan using an in-transit cold treatment protocol of 14 d or 12 d at temperatures <0 degrees c for treatment of ceratitis capitata (wiedemann) (diptera: tephritidae) in 'clementine' mandarins (citrus reticulata blanco) and other citrus types, respectively. to reduce the risk of chilling injury with this treatment, research was conducted with temperatures >0 degrees c. earlier south african research had shown that young (6-d-old) ...	2011	21882680
cold susceptibility and disinfestation of bactrocera invadens (diptera: tephritidae) in oranges.	to develop a cold disinfestation treatment for the fruit fly bactrocera invadens drew, tsuruta & white (diptera: tephritidae) that is rapidly spreading across africa, research was conducted in nairobi, kenya, using flies from a laboratory culture and 'valencia' orange (citrus sinensis l. osbeck) as the host. the developmental rate of b. invadens in valencia oranges was determined at 28 degrees c, and the third instar was found to be the least susceptible of the egg and larval life stages to cold ...	2011	21882681
temperature thresholds and degree-day model for marmara gulosa (lepidoptera: gracillariidae).	the developmental thresholds for marmara gulosa guillén & davis (lepidoptera: gracillariidae) were investigated in the laboratory by using 17, 21, 25, 29, and 33 degrees c. the lowest mortality occurred in cohorts exposed to 25 and 29 degrees c. other temperatures caused >10% mortality primarily in egg and first and second instar sap-feeding larvae. linear regression analysis approximated the lower developmental threshold at 12.2 degrees c. high mortality and slow developmental rate at 33 degree ...	2011	21882694
Comparison of ascorbate metabolism in fruits of two citrus species with obvious difference in ascorbate content in pulp.	Citrus fruit is widely consumed and provides ascorbate for human health. The ascorbate content in pulp is generally higher in orange (Citrus sinensis Osb.) than in Satsuma mandarin (Citrus unshiu Marc.). However, what contributes to such difference is still unknown. In the present study, ascorbate accumulation, expression profiles of genes involved in L-galactose pathway and activity changes of enzymes related with L-ascorbic acid (AA) oxidation and recycling were investigated during fruit devel ...	2011	21925761
metal analysis in citrus sinensis fruit peel and psidium guajava leaf.	the determination of metal traces is very important because they are involved in biological cycles and indicate high toxicity. the objective of the present study is to measure the levels of heavy metals and mineral ions in medicinally important plant species, citrus sinensis and psidium guajava. this study investigates the accumulation of copper (cu), zinc (zn), cadmium (cd), aluminum (al), mercury (hg), arsenic (as), selenium (se) and inorganic minerals like calcium (ca) and magnesium (mg) in c ...	2011	21976824
glycaemic response to some commonly eaten fruits in type 2 diabetes mellitus.	it is not known which of the commonly consumed fruits in nigeria are suitable for persons with diabetes mellitus especially with regards to the attendant plasma glucose response (pgr) to consumption of such fruits.	2011	21984455
Regulation of carotenoid accumulation and the expression of carotenoid metabolic genes in citrus juice sacs in vitro.	In the present study, to investigate the mechanisms regulating carotenoid accumulation in citrus, a culture system was set up in vitro with juice sacs of three citrus varieties, Satsuma mandarin (Citrus unshiu Marc.), Valencia orange (Citrus sinensis Osbeck), and Lisbon lemon (Citrus limon Burm.f.). The juice sacs of all the three varieties enlarged gradually with carotenoid accumulation. The changing patterns of carotenoid content and the expression of carotenoid metabolic genes in juice sacs i ...	2011	21994171
interaction of hesperetin glucuronide conjugates with human bcrp, mrp2 and mrp3 as detected in membrane vesicles of overexpressing baculovirus-infected sf9 cells.	the citrus flavonoid hesperetin (4'-methoxy-3',5,7-trihydroxyflavanone) is the aglycone of hesperidin, the major flavonoid present in sweet oranges. hesperetin 7-o-glucuronide (h7g) and hesperetin 3'-o-glucuronide (h3'g) are the two most abundant metabolites of hesperetin in vivo. in this study, their interaction with specific abc transporters, believed to play a role in the disposition and bioavailability of hesperetin, was studied using sf9 membranes from cells overexpressing human bcrp (abcg2 ...	2011	22083890
insecticidal evaluation of essential oils of citrus sinensis l. (myrtales: myrtaceae) against housefly, musca domestica l. (diptera: muscidae).	the housefly, musca domestica l., is one of the most common insects, associated with vectoring of various etiological agents. in order to search for effective control agent, the essential oil of sweet orange [citrus sinensis (l.) osbeck] was evaluated for its insecticidal activity against the larvae and pupae of housefly using contact toxicity and fumigation bioassays. in the contact toxicity assay, lethal concentration, lc(50) of c. sinensis essential oil against housefly larvae, varied between ...	2012	22127387
neurotrophic effect of citrus 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone: promotion of neurite outgrowth via camp/pka/creb pathway in pc12 cells.	5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone (5-oh-hxmf), a hydroxylated polymethoxyflavone, is found exclusively in the citrus genus, particularly in the peels of sweet orange. in this research, we report the first investigation of the neurotrophic effects and mechanism of 5-oh-hxmf in pc12 pheochromocytoma cells. we found that 5-oh-hxmf can effectively induce pc12 neurite outgrowth accompanied with the expression of neuronal differentiation marker protein growth-associated protein-43(gap-43). 5- ...	2011	22140566
comparative use of indel and ssr markers in deciphering the interspecific structure of cultivated citrus genetic diversity: a perspective for genetic association studies.	genetic stratification associated with domestication history is a key parameter for estimating the pertinence of genetic association study within a gene pool. previous molecular and phenotypic studies have shown that most of the diversity of cultivated citrus results from recombination between three main species: c. medica (citron), c. reticulata (mandarin) and c. maxima (pummelo). however, the precise contribution of each of these basic species to the genomes of secondary cultivated species, su ...	2012	22160318
characterization and comparison of volatile constituents of juice and peel from clementine, mandarin and their hybrids.	the volatile compositions of juice and peel of clementine (citrus reticulata x citrus sinensis var. commune), mandarin (citrus reticulata blanco var. willow leaf) and their hybrids were analyzed by headspace solid-phase extraction (hs-spme) coupled with gas chromatography and either a flame ionization detector (fid) or a mass spectrometer (ms). the major compounds of the volatile fractions of clementine and mandarin were limonene and limonene/gamma-terpinene, respectively. the volatile compositi ...	2011	22164792
transcriptome changes during fruit development and ripening of sweet orange (citrus sinensis).	the transcriptome of the fruit pulp of the sweet orange variety anliu (wt) and that of its red fleshed mutant hong anliu (mt) were compared to understand the dynamics and differential expression of genes expressed during fruit development and ripening.	2012	22230690
snp mining in c. clementina bac end sequences; transferability in the citrus genus (rutaceae), phylogenetic inferences and perspectives for genetic mapping.	with the increasing availability of est databases and whole genome sequences, snps have become the most abundant and powerful polymorphic markers. however, snp chip data generally suffers from ascertainment biases caused by the snp discovery and selection process in which a small number of individuals are used as discovery panels. the ongoing international citrus genome consortium sequencing project of the highly heterozygous clementine and sweet orange genomes will soon result in the release of ...	2012	22233093
electronic nose application for determination of penicillium digitatum in valencia oranges.	penicillium digitatum and penicillium italicum are responsible for one the most serious diseases occurring during storage of citrus fruits. its early detection allows a relevant increase in shelf life, and in situ monitoring of fungal infections represents a very efficient tool to improve storage quality. in the case of metabolic alterations due to physiological or fungal pathologies, olfactometric analysis allows the detection of specific volatile biomarkers, thus providing an effective tool fo ...	2012	22261834
efficacy of killer yeasts in the biological control of penicillium digitatum on tarocco orange fruits (citrus sinensis).	killer saccharomyces cerevisiae and wickerhamomyces anomalus yeast strains were tested as biocontrol agents against penicillium digitatum, one the most important causes of postharvest decay in orange fruits. w. anomalus, grown on acidified medium, demonstrated micocinogenic activity against p. digitatum, as indicated by large inhibition halos and hyphal damage resulting from β-glucanase activity. oranges that had been deliberately inoculated with pathogens were protected from decay by w. anomalu ...	2012	22265304
melanogenesis of murine melanoma cells induced by hesperetin, a citrus hydrolysate-derived flavonoid.	melanogenesis is a complex process that modulates skin pigmentation to defend photodamage. citrus is the most widely produced fruit crop in the world. people ingest various citrus fruits in their common diets. in the present study, the acid-hydrolyzed and un-hydrolyzed extracts of orange-type citrus fruits were subjected to analyze flavonoid compositions and assess their effects on melanin synthesis in murine b16-f10 melanoma cells. the acid-hydrolyzed extracts of citrus sinensis, c. reticulata, ...	2012	22266363
efficacy of herbal essential oils as insecticide against aedes aegypti (linn.), culex quinquefasciatus (say) and anopheles dirus (peyton and harrison).	the essential oils of cananga odorata (ylang ylang), citrus sinensis (orange), cymbopogon citratus (lemongrass), cymbopogon nardus (citronella grass), eucalyptus citriodora (eucalyptus), ocimum basilicum (sweet basil) and syzygium aromaticum (clove), were tested for their insecticide activity against aedes aegypti, culex quinquefasciatus and anopheles dirus using the who standard susceptibility test. these were applied in soybean oil at dose of 1%, 5% and 10% (w/v). c. citratus had the kt, value ...	2011	22299433
differential expression of genes involved in alternative glycolytic pathways, phosphorus scavenging and recycling in response to aluminum and phosphorus interactions in citrus roots.	the objective was to determine the possible links between the expression levels of genes involved in alternative glycolytic pathways, phosphorus (p) scavenging and recycling and citrus tolerance to aluminum (al) and/or p-deficiency. 'xuegan' (citrus sinensis) and 'sour pummelo' (citrus grandis) seedlings were irrigated for 18 weeks with nutrient solution containing 0 and 1.2 mm alcl(3)·6h(2)o × 0, 50 and 200 μm kh(2)po(4). c. sinensis displayed more tolerant to al and p-deficiency than c. grandi ...	2012	22307782
unravelling molecular responses to moderate dehydration in harvested fruit of sweet orange (citrus sinensis l. osbeck) using a fruit-specific aba-deficient mutant.	water stress affects many agronomic traits that may be regulated by the phytohormone abscisic acid (aba). within these traits, loss of fruit quality becomes important in many citrus cultivars that develop peel damage in response to dehydration. to study peel dehydration transcriptional responsiveness in harvested citrus fruit and the putative role of aba in this process, this study performed a comparative large-scale transcriptional analysis of water-stressed fruits of the wild-type navelate ora ...	2012	22315241
ozone deposition to an orange orchard: partitioning between stomatal and non-stomatal sinks.	orange trees are widely cultivated in regions with high concentrations of tropospheric ozone. citrus absorb ozone through their stomata and emit volatile organic compounds (voc), which, together with soil emissions of no, contribute to non-stomatal ozone removal. in a valencia orange orchard in exeter, california, we used fast sensors and eddy covariance to characterize water and ozone fluxes. we also measured meteorological parameters necessary to model other important sinks of ozone deposition ...	2012	22341155
volatile constituents of wild citrus mangshanyegan (citrus nobilis lauriro) peel oil.	volatiles of a wild mandarin, mangshanyegan (citrus nobilis lauriro), were characterized by gc-ms, and their aroma active compounds were identified by aroma extract dilution analysis (aeda) and gas chromatography-olfactometry (gc-o). the volatile profile of mangshanyegan was compared with those of other four citrus species, kaopan pummelo (citrus grandis), eureka lemon (citrus limon), huangyanbendizao tangerine (citrus reticulata), and seike navel orange (citrus sinensis). monoterpene hydrocarbo ...	2012	22352344
infrared spectroscopy: a potential tool in huanglongbing and citrus variegated chlorosis diagnosis.	huanglongbing (hlb) and citrus variegated chlorosis (cvc) are serious threats to citrus production and have caused considerable economic losses worldwide, especially in brazil, which is one of the biggest citrus producers in the world. neither disease has a cure nor an efficient means of control. they are also generally confused with each other in the field since they share similar initial symptoms, e.g., yellowing blotchy leaves. the most efficient tool for detecting these diseases is by polyme ...	2012	22365672
antimicrobial effect and mode of action of terpeneless cold-pressed valencia orange essential oil on methicillin-resistant staphylococcus aureus.	the objectives of this study were to evaluate the antistaphylococcal effect and elucidate the mechanism of action of orange essential oil against antibiotic-resistant staphylococcus aureus strains.	2012	22372962
enhanced inhibition of listeria monocytogenes by a combination of cold pressed terpeneless valencia orange oil and antibiotics.	this study was designed to evaluate the ability of cold pressed terpeneless valencia orange oil (cptvo) to enhance the effectiveness of antibiotics against 10 strains of listeria monocytogenes. disc diffusion assays were performed to determine the effects of cptvo and two antibiotics with different mechanisms of action (i.e., penicillin and chloramphenicol) individually and in combination with cptvo. cptvo alone produced zones ranging from 16.5 to 19.9 mm. penicillin at 2 or 10 units produced zo ...	2012	22394025
inhibition of acetylcholinesterase activity and fe2+-induced lipid peroxidation in rat brain in vitro by some citrus fruit juices.	this study sought to characterize the effects of some citrus fruit juices (shaddock [citrus maxima], grapefruit [citrus paradisii], lemon [citrus limoni], orange [citrus sinensis], and tangerine [citrus reticulata]) on acetylcholinesterase activity in vitro. the total phenolic content, radical scavenging abilities, and inhibition of fe(2+)-induced malondialdehyde (mda) production in rats brain homogenate in vitro were also assessed. orange had significantly (p<.05) higher phenolic content than t ...	2012	22400910
gene expression in citrus sinensis fruit tissues harvested from huanglongbing-infected trees: comparison with girdled fruit.	distribution of viable candidatus liberibacter asiaticus (calas) in sweet orange fruit and leaves ('hamlin' and 'valencia') and transcriptomic changes associated with huanglongbing (hlb) infection in fruit tissues are reported. viable calas was present in most fruit tissues tested in hlb trees, with the highest titre detected in vascular tissue near the calyx abscission zone. transcriptomic changes associated with hlb infection were analysed in flavedo (ff), vascular tissue (vt), and juice vesic ...	2012	22407645
effects of cinnamon leaf, oregano and sweet orange essential oils on fermentation and aerobic stability of barley silage.	silage additives are marketed with the primary aim of improving the fermentation and/or aerobic stability of silage. the objective of this study was to evaluate the impact of three different essential oils (eos; cinnamon leaf (cin), oregano (ore) and sweet orange (so)) on the fermentation characteristics and stability of barley silage. chopped whole-plant barley (hordem vulgare l.) forage was ensiled either untreated (0 mg kg⁻¹ dry matter (dm)) or treated with cin, ore or so (37.5, 75 and 120 mg ...	2012	22413147
activity of citrus essential oils against escherichia coli o157:h7 and salmonella spp. and effects on beef subprimal cuts under refrigeration.	escherichia coli o157:h7 and salmonella spp. are bacterial pathogens often associated with beef, and cause many cases of foodborne illness each year in the united states. during beef slaughter and processing, these bacteria may spread from the hide or intestines to the carcass. the objective of this research was to investigate the use of naturally occurring compounds citrus essential oils (ceos) extracted from orange peel to reduce or eliminate these pathogens at the chilling stage of processing ...	2011	22417514
chemometric characterization of fruit juices from spanish cultivars according to their phenolic compound contents: i. citrus fruits.	the data set composed by phenolic compound profiles of 83 citrus juices (determined by hplc-dad-ms/ms) was evaluated by chemometrics to differentiate them according to citrus species (sweet orange, tangerine, lemon, and grapefruit). cluster analysis (ca) and principal component analysis (pca) showed natural sample grouping among citrus species and even the citrus subclass. most of the information contained in the full data set can be captured if only 15 phenolic compounds (concentration ≥10 mg/l ...	2012	22423971
retrotransposons control fruit-specific, cold-dependent accumulation of anthocyanins in blood oranges.	traditionally, sicilian blood oranges (citrus sinensis) have been associated with cardiovascular health, and consumption has been shown to prevent obesity in mice fed a high-fat diet. despite increasing consumer interest in these health-promoting attributes, production of blood oranges remains unreliable due largely to a dependency on cold for full color formation. we show that sicilian blood orange arose by insertion of a copia-like retrotransposon adjacent to a gene encoding ruby, a myb transc ...	2012	22427337
assessment of the genetic diversity of the tunisian citrus rootstock germplasm.	citrus represents a substantial income for farmers in the mediterranean basin. however, the mediterranean citrus industry faces increasing biotic and abiotic constraints. therefore the breeding and selection of new rootstocks are now of the utmost importance. in tunisia, in addition to sour orange, the most widespread traditional rootstock of the mediterranean area, other citrus rootstocks and well adapted to local environmental conditions, are traditionally used and should be important genetic ...	2012	22429788
mistletoes and mutant albino shoots on woody plants as mineral nutrient traps.	potassium, sulphur and zinc contents of mistletoe leaves are generally higher than in their hosts. this is attributed to the fact that chemical elements which are cycled between xylem and phloem in the process of phloem loading of sugars are trapped in the mistletoe, because these parasites do not feed their hosts. here it is hypothesized that mutant albino shoots on otherwise green plants should behave similarly, because they lack photosynthesis and thus cannot recycle elements involved in suga ...	2012	22442343
an integrative analysis of transcriptome and proteome provides new insights into carotenoid biosynthesis and regulation in sweet orange fruits.	an integrative analysis of transcriptome and proteome was performed to identify differential genes/proteins of a red-flesh sweet orange cara cara in comparison with a common cultivar newhall at ripening stages. at the transcript level, gene expression was measured with massively parallel signature sequencing (mpss), and 629 genes of these two sweet orange cultivars differed by two fold or more (fdr<0.001). at the protein level, a combination of 2de and maldi-tof-tof ms identified 48 protein spot ...	2012	22472342
generation, functional analysis and utility of citrus grandis est from a flower-derived cdna library.	pummelo (citrus grandis) is one of the most important species found in the genus citrus and one of the ancestors of sweet oranges. we used flower buds at different developmental stages to construct the first cdna library for this species. a total of 3,758 est sequences were generated from the cdna library and clustered into 2,228 unigenes, comprising 451 contigs and 1,777 singletons. among these unigene sequences, 1,266 have significant homology to the non-redundant protein database, from which ...	2012	22477149
[application of hyperspectral fluorescence image technology in detection of early rotten oranges].	rottenness is most prevalent and devastating disease that threats citrus fruit. automatic detection of early rottenness can enhance the competitiveness and profitability of the citrus industry. however, there is no efficient automatic detection technology at this time that could detect this disease. the navel orange was selected as research objective. hyperspectral fluorescence imaging was used to detect early rottenness in orange. optimum index factor (oif) method was applied to identify the op ...	2012	22497146
structure-activity relationship of citrus polymethoxylated flavones and their inhibitory effects on aspergillus niger.	citrus peels are rich in polymethoxylated flavones (pmfs) and are potential sources of natural preservatives. six pmfs extracts, isolated and purified from the peels of three mandarins (citrus reticulata) and three sweet oranges (citrus sinensis), were identified and quantitated. their inhibitory effects on aspergillus niger were evaluated using a microbroth dilution assay. the red tangerine variety exhibited the greatest antifungal activity (mic = 0.2 mg/ml), while jincheng showed the lowest ac ...	2012	22500738
the effects of herbal essential oils on the oviposition-deterrent and ovicidal activities of aedes aegypti (linn.), anopheles dirus (peyton and harrison) and culex quinquefasciatus (say).	the effect of oviposition-deterrent and ovicidal of seven essential oils were evaluated towards three mosquito vectors, aedes aegypti, anopheles dirus and culex quinquefasciatus. the oviposition activity index (oai) values of six essential oils namely cananga odorata, cymbopogon citratus, cymbopogon nardus, eucalyptus citriodora, ocimum basilicum and syzygium aromaticum indicated that there were more deterrent than the control whereas citrus sinensis oil acted as oviposition attractant. at highe ...	2012	22543614
in vitro effects of citrus oils against mycobacterium tuberculosis and non-tuberculous mycobacteria of clinical importance.	we evaluated the in vitro activity of citrus oils against mycobacterium tuberculosis and other non-tuberculous mycobacterium species. citrus essential oils were tested against a variety of mycobacterium species and strains using the bactec radiometric growth system. cold pressed terpeneless valencia oil (cpt) was further tested using the wayne model of in vitro latency. exposure of m. tuberculosis and m. bovis bcg to 0.025 % cold pressed terpeneless valencia orange oil (cpt) resulted in a 3-log ...	2012	22560037
carotenoid accumulation in postharvest "cara cara" navel orange (citrus sinensis osbeck) fruits stored at different temperatures was transcriptionally regulated in a tissue-dependent manner.	the main objective of this work was to investigate the effect of storage temperature (4 and 20 °c) on carotenoid accumulation and on the expression levels of seven carotenoid biosynthetic genes (psy, pds, zds, lcyb, lcye, hyb and zep) in postharvest 'cara cara' navel orange (c. sinensis osbeck) fruits. storage at 20 °c rapidly increased the carotenoid content in the peel, whereas the content remained unchanged in the pulp before 35 days of storage. by contrast, storage at 4 °c maintained the car ...	2012	22562781
inhibition of beef isolates of e. coli o157:h7 by orange oil at various temperatures.	plant essential oils have previously been shown to exhibit antimicrobial activities against various microorganisms. in this study, cold pressed terpeneless valencia orange oil (cptvo) was examined at various temperatures (37, 10, and 4 °c) to determine its antimicrobial activity against 3 strains of e. coli o157:h7 recovered from beef products. a micro broth dilution method using 96 well microtiter plates was used with trypticase soy broth with 0.15% agar and 2,3,5 tetrazolium chloride as a grow ...	2012	22583064
validation and characterization of citrus sinensis micrornas and their target genes.	micrornas play vital role in plant growth and development by changeable expression of their target genes with most plant micrornas having perfect or near-perfect complementarities with their target genes but mirnas in citrus sinensis (csi-mirnas) and their function have not been widely studied.	2012	22583737
weedy hosts and prevalence of potential leafhopper vectors (hemiptera: cicadellidae) of a phytoplasma (16srix group) associated with huanglongbing symptoms in citrus groves.	huanglongbing (hlb) is a severe citrus (citrus spp.) disease associated with the bacteria genus candidatus liberibacter, detected in brazil in 2004. another bacterium was found in association with hlb symptoms and characterized as a phytoplasma belonging to the 16srix group. the objectives of this study were to identify potential leafhopper vectors of the hlb-associated phytoplasma and their host plants. leafhoppers were sampled every other week for 12 mo with sticky yellow cards placed at two h ...	2012	22606800
identification of differentially expressed genes in a spontaneous altered leaf shape mutant of the navel orange [citrus sinensis (l.) osbeck].	most of the economically important citrus cultivars have originated from bud mutations. leaf shape and structure are important factors that impact plant photosynthesis. we found a spontaneous bud mutant exhibiting a narrow leaf phenotype in navel orange [citrus sinensis (l.) osbeck]. to identify and characterize the genes involved in the formation of this trait, we performed suppression subtractive hybridization (ssh) and macroarray analysis. a total of 221 non-redundant differentially expressed ...	2012	22609459
transcriptional profiling of genes involved in embryogenic, non-embryogenic calluses and somatic embryogenesis of valencia sweet orange by ssh-based microarray.	somatic embryogenesis (se) is a most promising technology that is used for in vitro germplasm conservation and genetic improvement via biotechnological approaches in citrus. herein, three suppression subtractive hybridization (ssh) libraries were constructed using calluses of citrus sinensis cv. 'valencia' to explore the molecular mechanisms that underlie the se in citrus. a total of 880 unisequences were identified by microarray screening based on these three ssh libraries. gene ontology analys ...	2012	22622359
changes of peel essential oil composition of four tunisian citrus during fruit maturation.	the present work investigates the effect of ripening stage on the chemical composition of essential oil extracted from peel of four citrus: bitter orange (citrus aurantium), lemon (citrus limon), orange maltaise (citrus sinensis), and mandarin (citrus reticulate) and on their antibacterial activity. essential oils yields varied during ripening from 0.46 to 2.70%, where mandarin was found to be the richest. forty volatile compounds were identified. limonene (67.90-90.95%) and 1,8-cineole (tr-14.7 ...	2012	22645427
use of honey bees (apis mellifera l.) to detect the presence of mediterranean fruit fly (ceratitis capitata wiedemann) larvae in valencia oranges.	when fruit deteriorates a characteristic profile of volatile chemicals is produced that is different from that produced by healthy fruits. the identification of such chemicals allows the possibility of monitoring the fruit for early signs of deterioration with biological sensors. the use of honey bees and other insects as biological sensors is well known. this study aimed to identify the volatiles produced by oranges infested with larvae of the mediterranean fruit fly and to test the ability of ...	2012	22653619
exogenous treatment with salicylic acid attenuates occurrence of citrus canker in susceptible navel orange (citrus sinensis osbeck).	citrus canker caused by xanthomonas axonopodis pv. citri (xac) is a devastating bacterial disease threatening the citrus industry. salicylic acid (sa) plays a key role in plant defense response to biotic stress, but information is scarce concerning the application of sa to enhancing xac resistance. in the present research attempts were made to investigate how exogenous application of sa influenced canker disease outbreak in navel orange (citrus sinensis). exogenously applied sa at 0.25 mm signif ...	2012	22658220
citrus and prunuscopia-like retrotransposons.	many of the world's most important citrus cultivars ("washington navel", satsumas, clementines) have arisen through somatic mutation. this phenomenon occurs fairly often in the various species and varieties of the genus.the presence of copia-like retrotransposons has been investigated in fruit trees, especially citrus, by using a pcr assay designed to detect copia-like reverse transcriptase (rt) sequences. amplification products from a genotype of each the following species citrus sinensis, citr ...	1999	22665184
genetic diversity and population differentiation of the causal agent of citrus black spot in brazil.	one of the most important diseases that affect sweet orange orchards in brazil is the citrus black spot that is caused by the fungus guignardia citricarpa. this disease causes irreparable losses due to the premature falling of fruit, as well as its severe effects on the epidermis of ripe fruit that renders them unacceptable at the fresh fruit markets. despite the fact that the fungus and the disease are well studied, little is known about the genetic diversity and the structure of the fungi popu ...	2012	22666111
construction of egfp-labeling system for visualizing the infection process of xanthomonas axonopodis pv. citri in planta.	xanthomonas axonopodis pv. citri (xac) is the causal agent of citrus bacterial canker, an economically important disease to world citrus industry. to monitor the infection process of xac in different citrus plants, the enhanced green florescent protein (egfp) visualizing system was constructed to visualize the propagation and localization in planta. first, the wild-type xac was isolated from the diseased leaves of susceptible 'bingtang' sweet orange, and then the isolated xac was labeled with eg ...	2012	22674174
a lov protein modulates the physiological attributes of xanthomonas axonopodis pv. citri relevant for host plant colonization.	recent studies have demonstrated that an appropriate light environment is required for the establishment of efficient vegetal resistance responses in several plant-pathogen interactions. the photoreceptors implicated in such responses are mainly those belonging to the phytochrome family. data obtained from bacterial genome sequences revealed the presence of photosensory proteins of the bluf (blue light sensing using fad), lov (light, oxygen, voltage) and phytochrome families with no known functi ...	2012	22675525
phylogeographic structure, outbreeding depression, and reluctant virgin oviposition in the bean thrips, caliothrips fasciatus (pergande) (thysanoptera: thripidae), in california.	bean thrips, caliothrips fasciatus, is native to western north america. once considered a pest of several crops in its native area, its pest status has waned over recent decades. however, due to its habit of aggregating in the navel of navel oranges, bean thrips remains economically important because some countries importing oranges from california have designated it a quarantine pest. despite continued propagule pressure, bean thrips has never established outside north america. we examined gene ...	2012	22676974
quality assessment of industrially processed fruit juices available in dhaka city, bangladesh.	this study was performed to evaluate the nutritional and microbiological quality of industrially processed packed fruit juices of mango (mangifera indica) and orange (citrus sinensis) from nine different manufacturing companies in dhaka city. the highest quantity of total sugar (17.62%) and reducing sugar (9.99%) was recorded in mango juices while the lowest in orange juices (10.41% and 2.24% respectively) of different companies. in this study, protein contents were comparatively higher in mango ...	2010	22691996
expression and functional analysis of two lycopene β-cyclases from citrus fruits.	in the present study, two lcyb genes (citlcyb1 and citlcyb2) were isolated from satsuma mandarin (citrus unshiu marc.), valencia orange (citrus sinensis osbeck) and lisbon lemon (citrus limon burm.f.) and their functions were analyzed by the color complementation assay in lycopene-accumulating e. coli cells. the results showed that citlcyb1 and citlcyb2 shared high identity at the amino acid level among the three citrus varieties. the n-terminal region of the two proteins encoded by citlcyb1 and ...	2012	22729824
field performance of transgenic citrus trees: assessment of the long-term expression of uida and nptii transgenes and its impact on relevant agronomic and phenotypic characteristics.	the future of genetic transformation as a tool for the improvement of fruit trees depends on the development of proper systems for the assessment of unintended effects in field-grown gm lines. in this study, we used eight transgenic lines of two different citrus types (sweet orange and citrange) transformed with the marker genes β-glucuronidase (uida) and neomycin phosphotransferase ii (nptii) as model systems to study for the first time in citrus the long-term stability of transgene expression ...	2012	22794278
larvicidal, pupicidal, repellent and adulticidal activity of citrus sinensis orange peel extract against anopheles stephensi, aedes aegypti and culex quinquefasciatus (diptera: culicidae).	mosquitoes are the carriers of severe and well-known illnesses such as malaria, arboviral encephalitis, dengue fever, chikunguniya fever, west nile virus and yellow fever. these diseases produce significant morbidity and mortality in humans and livestock around the world. the present study explored the effects of orange peel ethanol extract of citrus sinensis on larvicidal, pupicidal, repellent and adulticidal activity against anopheles stephensi, aedes aegypti and culex quinquefasciatus. the or ...	2012	22797605
comparison of antifungal activities of vietnamese citrus essential oils.	citrus essential oils (eos) are volatile compounds from citrus peels and widely used in perfumes, cosmetics, soaps and aromatherapy. in this study, inhibition of citrus eos extracted from vietnamese orange (citrus sinensis), mandarin (citrus reticulata blanco), pomelo (citrus grandis osbeck) and lime (citrus aurantifolia swingle) on the growth of plant pathogenic fungi, mucor hiemalis, penicillium expansum and fusarium proliferatum was investigated. the eos of the citrus peels were obtained by c ...	2013	22799453
gus expression in sweet oranges (citrus sinensis l. osbeck) driven by three different phloem-specific promoters.	huanglongbing (hlb) is associated with candidatus liberibacter spp., endogenous, sieve tube-restricted bacteria that are transmitted by citrus psyllid insect vectors. transgenic expression in the phloem of specific genes that might affect ca. liberibacter spp. growth and development may be an adequate strategy to improve citrus resistance to hlb. to study specific phloem gene expression in citrus, we developed three different binary vector constructs with expression cassettes bearing the β-glucu ...	2012	22801867
the influence of citrus rootstocks on the relationship between the mite brevipalpus phoenicis and citrus leprosis disease.	leprosis is one of the most serious citrus plant diseases. leprosis-affected plants, especially sweet orange [citrus sinensis (l.) osbeck], which is the most widely cultivated citrus fruit worldwide, show reduced photosynthetic capacity and severe defoliation. the aim was to evaluate the relationship between the brevipalpus phoenicis (geijskes) vector mite and citrus leprosis disease in pera sweet orange plants grafted on different rootstocks. data were analysed using numerical classification an ...	2013	22807324
comparative transcriptional and anatomical analyses of tolerant rough lemon and susceptible sweet orange in response to 'candidatus liberibacter asiaticus' infection.	although there are no known sources of genetic resistance, some citrus spp. are reportedly tolerant to huanglongbing (hlb), presumably caused by 'candidatus liberibacter asiaticus'. time-course transcriptional analysis of tolerant rough lemon (citrus jambhiri) and susceptible sweet orange (c. sinensis) in response to 'ca. l. asiaticus' infection showed more genes differentially expressed in hlb-affected rough lemon than sweet orange at early stages but substantially fewer at late time points, po ...	2012	22809274
different transcriptional response to xanthomonas citri subsp. citri between kumquat and sweet orange with contrasting canker tolerance.	citrus canker disease caused by xanthomonas citri subsp. citri (xcc) is one of the most devastating biotic stresses affecting the citrus industry. meiwa kumquat (fortunella crassifolia) is canker-resistant, while newhall navel orange (citrus sinensis osbeck) is canker-sensitive. to understand the molecular mechanisms underlying the differences in responses to xcc, transcriptomic profiles of these two genotypes following xcc attack were compared by using the affymetrix citrus genome genechip. a t ...	2012	22848606
effect of sweet orange aroma on experimental anxiety in humans.	the objective of this study was to evaluate the potential anxiolytic effect of sweet orange (citrus sinensis) aroma in healthy volunteers submitted to an anxiogenic situation.	2012	22849536
potential of moringa oleifera root and citrus sinensis fruit rind extracts in the treatment of ulcerative colitis in mice.	the plant moringa oleifera lam (moringaceae), commonly known as the drumstick tree, is an indigenous species in india. this species has been of interest to researchers because traditionally its roots are reported in the treatment of ulcerative colitis (uc). traditionally it is reported that citrus sinensis linn (rutaceae) fruit rind when combined with m. oleifera will increase the efficacy of the plant in the treatment of uc.	2012	22849565
effects of foliar application with compost tea and filtrate biogas slurry liquid on yield and fruit quality of washington navel orange (citrus sinenesis osbeck) trees.	sixteen-year-old navel orange trees at a private orchard located in kafer el-sheikh governorate, egypt, were used in this study. compost tea (ct) and filtrate biogas slurry liquid (flb) were applied at two different concentrations (50% and 100%); control trees were sprayed with water trees treated with ct at 100% were the highest in yield, fruit weight, and vitamin c, whereas the highest percentage of fruit set, fruit number and soluble solid content (ssc), lowest fruit drop, and highest reducin ...	2012	22866578
the citrus aba signalosome: identification and transcriptional regulation during sweet orange fruit ripening and leaf dehydration.	the abscisic acid (aba) signalling core in plants include the cytosolic aba receptors (pyr/pyl/rcars), the clade-a type 2c protein phosphatases (pp2cas), and the subclass iii snf1-related protein kinases 2 (snrk2s). the aim of this work was to identify these aba perception system components in sweet orange and to determine the influence of endogenous aba on their transcriptional regulation during fruit development and ripening, taking advantage of the comparative analysis between a wild-type and ...	2012	22888124
a comparison of the ultrastructure and composition of fruits' cuticular wax from the wild-type 'newhall' navel orange (citrus sinensis [l.] osbeck cv. newhall) and its glossy mutant.	the altered ultrastructure and composition of cuticular wax from 'glossy newhall' (mt) fruits lead to its glossy phenotype. a novel mutant derived from the wild-type (wt) 'newhall' navel orange (citrus sinensis [l.] osbeck cv. newhall), named 'glossy newhall' (mt), which produced much more glossy fruits that were easily distinguishable from the wt fruits was characterized in this report. the total wax loads of both wt and mt fruits varied considerably during the fruit development. the most abund ...	2012	22892682
application of orange essential oil as an antistaphylococcal agent in a dressing model.	staphylococcus aureus is the pathogen most often and prevalently involved in skin and soft tissue infections. in recent decades outbreaks of methicillin-resistant s. aureus (mrsa) have created major problems for skin therapy, and burn and wound care units. topical antimicrobials are most important component of wound infection therapy. alternative therapies are being sought for treatment of mrsa and one area of interest is the use of essential oils. with the increasing interest in the use and app ...	2012	22894560
evaluation of bioefficacy of three citrus essential oils against the dengue vector aedes albopictus (diptera: culicidae) in correlation to their components enantiomeric distribution.	laboratory experiments were conducted to study the bioefficacy against ae. albopictus of three citrus essential oils, derived from peels of citrus sinensis, citrus limon, and citrus paradise and of their components. chiral gas chromatography analysis revealed the dominant occurrence of r-(+)-limonene and (-)-β-pinene in all three essential oils while in the case of lemon oil γ-terpinene, neral, and geranial detected also among other components. the tested citrus essential oils were toxic against ...	2012	22903418
a new strategy employed for identification of sweet orange cultivars with rapd markers.	we optimized rapd techniques by increasing the length of rapd primers and performing a strict screening of pcr annealing temperature to distinguish 60 sweet orange cultivars from the research institute of pomology at the chinese academy of agricultural sciences. a new approach called cultivar identification diagram (cid) was used to improve the efficiency of rapd markers for cultivar identification. thirteen effective primers were first screened from 54 rapd arbitrary 11-mer primers based on the ...	2012	22911591