Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| structure and composition of the assemblage of parasitoids associated to phyllocnistis citrella pupae stainton (lepidoptera: gracillariidae) in citrus orchards in southern brazil. | the structure and composition of the assemblage of pupal parasitoids of phyllocnistis citrella stainton, the citrus leafminer, were studied in two citrus orchards (citrus deliciosa tenore cv. montenegrina and citrus sinensis (l.) osbeck x citrus reticulata blanco hybrid murcott), in montenegro county (29 degrees 68's and 51 degrees 46'w), southern brazil. at fortnightly samplings, from july 2001 to june 2003, all the new shoots from 24 randomly selected trees were inspected. the species richness ... | 2007 | 18060301 |
| characterization of the most odor-active volatiles of orange wine made from a turkish cv. kozan (citrus sinensis l. osbeck). | the aroma-active compounds of cv. turkish kozan orange wine were analyzed by sensory and instrumental analyses. liquid-liquid extraction with dichloromethane was used for extraction of volatile components. according to sensory analysis, the aromatic extract obtained by liquid-liquid extraction was representative of orange wine odor. a total of 63 compounds were identified and quantified in orange wine. the results of the gas chromatography-olfactometry analysis showed that 35 odorous compounds w ... | 2008 | 18078317 |
| a novel bud mutation that confers abnormal patterns of lycopene accumulation in sweet orange fruit (citrus sinensis l. osbeck). | a novel, pleiotropic sweet orange (citrus sinensis l. osbeck) mutant, 'hong anliu', is described. this mutation causes carotenoid accumulation, high sugar, and low acid in the fruits. gas chromatographic analysis revealed that high sugar and low acid in the fruit were caused by the accumulation of sucrose and the deficiency of citric acid. the dominant carotenoid accumulated in albedo, segment membranes, and juice sacs is lycopene, which can reach levels that are a 1000-fold higher than those in ... | 2007 | 18182424 |
| [use of morphological and physiological characters, and molecular markers to evaluate the genetic diversity of three clementine cultivars]. | originating from a natural crossing between mandarin and sweet orange at the end of the 19(th) century, clementine diversified through the selection of spontaneous mutations. today, it seems almost impossible to distinguish one variety from another. the development of molecular tools for variety identification is thus necessary. three clementine cultivars, representing distinct groups of fruit maturity, were evaluated. identification criteria were searched at the phenotypical level (organoleptic ... | 2008 | 18187117 |
| boron deficiency decreases growth and photosynthesis, and increases starch and hexoses in leaves of citrus seedlings. | seedlings of sweet orange (citrus sinensis) were fertilized for 14 weeks with boron (b)-free or b-sufficient (2.5 or 10 microm h(3)bo(3)) nutrient solution every other day. boron deficiency resulted in an overall inhibition of plant growth, with a reduction in root, stem and leaf dry weight (dw). boron-starved leaves showed decreased co(2) assimilation and stomatal conductance, but increased intercellular co(2) concentrations. activities of ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisc ... | 2008 | 18191499 |
| diversity and infectivity of arbuscular mycorrhizal fungi in agricultural soils of the sichuan province of mainland china. | knowledge about the presence and diversity of arbuscular mycorrhizal fungi (amf) in a specific area is an essential first step for utilizing these fungi in any application. the community composition of amf in intensively managed agricultural soil in the sichuan province of southwest china currently is unknown. in one set of samples, amf were trapped in pot cultures from 40 fields growing legumes in the panxi region, southeast sichuan. in a second set of samples, the mpn method with four-fold dil ... | 2008 | 18224350 |
| functional characterization of ap3, soc1 and wus homologues from citrus (citrus sinensis). | flowering and flower formation are defining features of angiosperms and the control of these developmental processes involves a common repertoire of genes which are shared among different species of flowering plants. these genes were first identified using various homeotic and flowering time mutants of arabidopsis and snapdragon, and homologous genes have subsequently been isolated from a wide range of different plant species based on the conservation of protein sequence and function. using dege ... | 2007 | 18251886 |
| [near infrared diffuse reflectance spectroscopy for rapid analysis of soluble solids content in navel orange]. | the potential of using visible and near infrared diffuse reflectance spectroscopy to assess soluble solids content (ssc) of intact navel orange was examined. a total 40 samples were used to develop the calibration and prediction models. nir spectral data were collected in the spectral region between 350 and 2 500 nm and their second derivative spectra were used for the present study. different scattering correction algorithms (no preprocessing and multiplicative scattering correction (msc)) were ... | 2007 | 18260391 |
| [effects of exogenous aba and ga3 on sugar concentration in flesh of cara cara navel orange]. | the concentration of glucose, fructose, sucrose and total sugar were determined after exogenous aba and ga3 treatment during young period of fruit and before fruit coloring in flesh of cara cara navel orange. the results showed that 10 mg x l(-1) aba treatment improved glucose, fructose and total sugar concentration significantly or very significantly, 50 mg x l(-1) aba treatment improved sucrose concentration very significantly, but 100 mg x l(-1) aba treatment reduced glucose concentration ver ... | 2007 | 18260446 |
| preference-performance linkage of the xylem feeding leafhopper, homalodisca vitripennis (hemiptera cicadellidae). | insect species exhibiting a weak linkage between adult preference and immature performance have frequently been shown to be prone to outbreaks. we used choice and no-choice tests to examine the preference-performance linkage of the xylem fluid-feeding leafhopper, homalodisca vitripennis germar. leafhoppers were offered a choice of hosts common to their native range and also a choice from hosts where they have been recently introduced. behavior (residence preference, oviposition preference, and c ... | 2007 | 18284780 |
| juice quality of two new mandarin-like hybrids (citrus clementina hort. ex tan x citrus sinensis l. osbeck) containing anthocyanins. | tacle and clara [monreal clementine (citrus clementina hort. ex tan) x tarocco orange (citrus sinensis l. osbeck)] are two new triploid citrus hybrids developed by the cra-istituto sperimentale per l'agrumicoltura (acireale, italy). the fruits are easy-peeling and juicy, have a pleasant taste like the tarocco orange, and are sweet like the monreal clementine. in addition, a distinctively attractive characteristic of these mandarin-like fruits is the red-pigmented flesh caused by the presence of ... | 2008 | 18298061 |
| evaluation of selection strategies alternative to nptii in genetic transformation of citrus. | the neomycin phosphotransferase (nptii) selection system has proved successful in citrus transformation; however, it may be recommendable to replace it given the pressure exerted against antibiotic-resistance selectable marker genes in transgenic plants. the present work investigates three different selection alternatives, comparing them to nptii selection in two citrus genotypes, carrizo citrange and pineapple sweet orange. the first method used the beta-glucuronidase (uida) reporter marker gen ... | 2008 | 18317775 |
| antioxidant enzymatic activity is linked to waterlogging stress tolerance in citrus. | soil flooding constitutes a seasonal factor that negatively affects plant performance and crop yields. in this work, the relationship between oxidative damage and flooding sensitivity was addressed in three citrus genotypes with different abilities to tolerate waterlogging. we examined leaf visible damage, oxidative damage in terms of malondialdehyde (mda) concentration, leaf proline concentration, leaf and root ascorbate and glutathione contents and the antioxidant enzyme activities superoxide ... | 2008 | 18333999 |
| distribution and quantification of candidatus liberibacter americanus, agent of huanglongbing disease of citrus in são paulo state, brasil, in leaves of an affected sweet orange tree as determined by pcr. | huanglongbing (hlb), an insect-transmitted disease of citrus, known for many years in asia and africa, has appeared in the state of são paulo state (ssp), brazil, in 2004, and the state of florida, usa, in 2005. hlb endangers the very existence of citrus, as trees infected with the bacterial pathogen, irrevocably decline. in the absence of curative procedures, control of hlb is difficult and only based on prevention. even though not available in culture, the hlb bacterium could be shown to be gr ... | 2008 | 18400468 |
| delay expression of limonoid udp-glucosyltransferase makes delayed bitterness in citrus. | genes encoding limonoid udp-glucosyltransferase from albedo of six citrus species with different levels of delayed bitterness are isolated and cloned in vector ptz57r/t. our results indicate that gene sequence of sweet lime (with intense juice delayed bitterness) have complete identity with satsuma mandarin (without distinctive juice delayed bitterness). also gene sequence of marsh seedless grapefruit, local orange and thompson navel orange (with mild juice delayed bitterness) have very similari ... | 2008 | 18407832 |
| antiperoxidative, antithyroidal, antihyperglycemic and cardioprotective role of citrus sinensis peel extract in male mice. | an extract of citrus sinensis (cs) peel was evaluated for its efficacy in ameliorating l-thyroxine (l-t(4)) induced tissue lipid peroxidation (lpo), hyperthyroidism and hyperglycemia in mice. in a preliminary investigation, of the three different doses of cs (12.5 mg/kg, 25 mg/kg and 50.0 mg/kg) peel extract, 25 mg/kg was found to be the most effective and antiperoxidative, while 50 mg/kg was proved to be hepatotoxic. therefore in the pilot experiment the effects of 25 mg/kg/day of cs for 10 day ... | 2008 | 18412146 |
| changes in carotenoid content and biosynthetic gene expression in juice sacs of four orange varieties (citrus sinensis) differing in flesh fruit color. | the contribution of carotenoid composition to the color range of the fruit juice sacs of four orange varieties (citrus sinensis) differing in flesh color, namely, shamouti (normal orange color), sanguinelli ("blood cultivar" purple color), cara cara navel (pink-reddish), and huang pi chen (yellowish color), was investigated. to this end, qualitative and quantitative analyses of carotenoid contents were first performed by high-performance liquid chromatography (hplc) using a c30 column and a phot ... | 2008 | 18433104 |
| involvement of polyamine biosynthesis in somatic embryogenesis of valencia sweet orange (citrus sinensis) induced by glycerol. | culture of citrus sinensis embryogenic callus on the embryo-inducing medium (eim) containing glycerol gave rise to a large number of embryos, whereas very few embryos were observed on the callus growth medium (cgm). in the current paper, attempts were made to investigate whether polyamine biosynthesis was involved in glycerol-mediated somatic embryogenesis. quantification of free polyamines by high-performance liquid chromatography showed that the cultures on eim had less putrescine than those o ... | 2009 | 18448195 |
| an evaluation of the basis and consequences of a stay-green mutation in the navel negra citrus mutant using transcriptomic and proteomic profiling and metabolite analysis. | a citrus sinensis spontaneous mutant, navel negra (nan), produces fruit with an abnormal brown-colored flavedo during ripening. analysis of pigment composition in the wild-type and nan flavedo suggested that typical ripening-related chlorophyll (chl) degradation, but not carotenoid biosynthesis, was impaired in the mutant, identifying nan as a type c stay-green mutant. nan exhibited normal expression of chl biosynthetic and catabolic genes and chlorophyllase activity but no accumulation of dephy ... | 2008 | 18467459 |
| spatial distribution of phyllocnistis citrella stainton (lepidoptera: gracillariidae) larvae in three scales. | the spatial distribution of larvae of phyllocnistis citrella stainton (lepidoptera: gracillariidae), the citrus leaf miner, in leaves, shoots, and tree crowns of montenegrina tangerine (citrus deliciosa tenore) and murcott tangor (citrus sinensis l. osbeck x citrus reticulata blanco) was determined. fortnightly samplings from july/2001 to june/2003, in orchards located in montenegro (29 degrees 68' s and 51 degrees 46' w), rs, brazil, were carried out. at each sampling occasion eighth shoots ran ... | 2008 | 18470399 |
| novel mechanism of modulating natural antioxidants in functional foods: involvement of plant growth promoting rhizobacteria nrrl b-30488. | the significance of plant growth-promoting rhizobacteria (pgpr) mediated increase in antioxidant potential in vegetables is yet unknown. the plant growth-promoting bacterium bacillus lentimorbus nrrl b-30488 (b-30488) mediated induction of dietary antioxidant in vegetables ( trigonella foenum-graecum, lactuca sativa, spinacia oleracea, and daucus carota) and fruit ( citrus sinensis) after minimal processing (fresh, boiled, and frozen) was tested by estimating the total phenol content, level of a ... | 2008 | 18491912 |
| phellodendron and citrus extracts benefit cardiovascular health in osteoarthritis patients: a double-blind, placebo-controlled pilot study. | the objective of this clinical study was to assess the potential benefit of a dietary supplement, np 06-1, on cardiovascular protective properties in overweight and normal weight adults diagnosed with osteoarthritis of the knee. | 2008 | 18492265 |
| regulation of carotenoid biosynthesis during fruit maturation in the red-fleshed orange mutant cara cara. | cara cara is a spontaneous bud mutation of navel orange (citrus. sinensis l. osbeck) characterized by developing fruits with a pulp of bright red coloration due to the presence of lycopene. peel of mutant fruits is however orange and indistinguishable from its parental. to elucidate the basis of lycopene accumulation in cara cara, we analyzed carotenoid profile and expression of three isoprenoid and nine carotenoid genes in flavedo and pulp of cara cara and navel fruits throughout development an ... | 2008 | 18538806 |
| [probability distribution of orthezia praelonga douglas (hemiptera: sternorrhyncha: ortheziidae) in citrus]. | the probability distribution of the orthezia scale, orthezia praelonga douglas, was studied in plots of commercial orange orchards (citrus sinensis l., var. "pêra rio"), with 5, 9 and 15 years of age, during one year. the survey of presence or absence of the pest in the plants was carried through twenty samplings in the total area of each plot visually inspecting each plant. the dispersion indexes used were: variance/mean relationship (i), index of morisita (id), coefficient of green (cx) and k ... | 2008 | 18575702 |
| effects of thermal treatments and storage on pectin methylesterase and peroxidase activity in freshly squeezed orange juice. | a specific indicator of freshness, allowing routine distinction between freshly squeezed orange juices (fsojs) and fsoj-like products, was to be identified. using the actijoule unit of a tubular heater at a flow rate of 60 l/h, fsojs from citrus sinensis (l.) osbeck cv. valencia late were continuously heated on a pilot plant scale at six different temperatures (42-92 degrees c), followed by continuous cooling to ambient temperature and subsequent filling into sterilized glass jars. the cloud sta ... | 2008 | 18582085 |
| a comparison of four dna extraction methods for the detection of citrus yellow mosaic badna virus from two species of citrus using pcr and dot-blot hybridization. | nucleic acid preparations extracted using four procedures were assessed to determine the suitability of the procedure for pcr-based and dna dot-blot-based detection of citrus yellow mosaic badna virus (cmbv) from two citrus species, acid lime and pummelo. it was found that the success of pcr detection depended upon the procedure of dna extraction whereas the dot-blot detection was successful with all extraction methods examined. cmbv dna sequences amplified from two citrus species indicated high ... | 2008 | 18582956 |
| characterization of aroma active compounds in fruit juice and peel oil of jinchen sweet orange fruit (citrus sinensis (l.) osbeck) by gc-ms and gc-o. | gas chromatography-mass spectrometry (gc-ms) and gas chromatography-olfactometry (gc-o) were used to determine the aromatic composition and aroma active compounds of fruit juice and peel oil of jinchen sweet orange fruit. totals of 49 and 32 compounds were identified in fruit juice and peel oil, respectively. gc-o was performed to study the aromatic profile of jinchen fruit juice and peel oil. a total of 41 components appeared to contribute to the aroma of fruit juice and peel oil. twelve compon ... | 2008 | 18596659 |
| medicinal values of fruit peels from citrus sinensis, punica granatum, and musa paradisiaca with respect to alterations in tissue lipid peroxidation and serum concentration of glucose, insulin, and thyroid hormones. | peel extracts from citrus sinensis, punica granatum, and musa paradisiaca were investigated for their effects on tissue lipid peroxidation (lpo) and on the concentration of thyroid hormones, insulin, and glucose in male rats. in vitro inhibition of h(2)o(2)-induced lpo in red blood cells of rats by 0.25, 0.50, 1.0, and 2.0 microg/ml c. sinensis, p. granatum, and m. paradisiaca peel extracts was observed in a dose-specific manner. maximum inhibition was observed at 0.50 microg/ml c. sinensis, 2.0 ... | 2008 | 18598183 |
| survival and development of immature stages of the mediterranean fruit fly (diptera: tephritidae) in citrus fruit. | we studied, under laboratory conditions, the performance of the mediterranean fruit fly, ceratitis capitata (wiedemann) (diptera: tephritidae), immature stages in intact whole fruit of three sweet orange varieties, lemon, and bitter oranges. both citrus variety and fruit part (flavedo, albedo, and pulp) had strong effects on larval performance, smaller effects on pupae, and no effects on eggs. fruit peel was the most critical parameter for larval development and survival, drastically affecting l ... | 2008 | 18613588 |
| in vitro evaluation of the effect of natural orange juices on dentin morphology. | the patient's diet has been considered an important etiological factor of dentin hypersensitivity. the frequent ingestion of acidic substances can promote the loss of dental structure or remove the smear layer. the purpose of this study was to evaluate the degree of smear layer removal and dentinal tubules exposure by different natural orange juices. extracted human teeth were submitted to manual scaling in order to develop the smear layer. seventy dentin samples were obtained and distributed in ... | 2008 | 18622489 |
| screening for antifeedant and larvicidal activity of plant extracts against helicoverpa armigera (hübner), sylepta derogata (f.) and anopheles stephensi (liston). | plant extracts, especially botanical insecticides, are currently studied more and more because of the possibility of their use in plant protection. biological activity of five solvent plant extracts were studied using fourth instar larvae of gram pod borer helicoverpa armigera (lepidoptera: noctuidae), cotton leaf roller sylepta derogata (lepidoptera: pyralidae) and malaria vector anopheles stephensi (diptera: culicidae). antifeedant and larvicidal activity of acetone, chloroform, ethyl acetate, ... | 2008 | 18679716 |
| chemical characterization of citrus sinensis grafted on c. limonia and the effect of some isolated compounds on the growth of xylella fastidiosa. | citrus sinensis grafted on c. limonia produces a considerable number of compounds that are not common in both plants developed from germination of seeds. the chemical profile of scion and rootstock differ notably for absence in the form of flavonoids and coumarins containing c5 prenyl groups attached to the carbon atoms of aromatic and heterocyclic systems or to oxygen. only linear pyranocoumarins xanthyletin and xanthoxyletin were found in scion. this observation indicates that the prenylated c ... | 2008 | 18683948 |
| gene expression analysis in citrus reveals the role of gibberellins on photosynthesis and stress. | the effect of gibberellins (ga) on internode transcriptome was investigated in transgenic carrizo citrange (citrus sinensis x poncirus trifoliata) plants overexpressing endogenous ccga20ox1 (encoding a ga biosynthetic gene), and in non-transformed explants treated with ga(3), using a citrus cdna microarray. substantial modulation of gene expression was found in sense ccga20ox plants. extensive up-regulation of genes involved in photosynthesis and carbon utilization, and down-regulation of those ... | 2008 | 18684239 |
| transmission efficiency of xylella fastidiosa by sharpshooters (hemiptera: cicadellidae) in coffee and citrus. | xylella fastidiosa (wells, raju, hung, weisburg, mandelco-paul, and brenner) is a bacterial pathogen transmitted by several sharpshooters in two tribes of cicadellinae (proconiini and cicadellini). here, we compared the transmission efficiency of x. fastidiosa in coffee (coffea arabica l.) and citrus [citrus sinensis (l.) osbeck] by cicadellini [bucephalogonia xanthophis (berg) and dilobopterus costalimai young] and proconiini [homalodisca ignorata melichar and oncometopia facialis (signoret)] s ... | 2008 | 18767717 |
| dispersion patterns and sampling plans for diaphorina citri (hemiptera: psyllidae) in citrus. | the abundance and spatial dispersion of diaphorina citri kuwayama (hemiptera: psyllidae) were studied in 34 grapefruit (citrus paradisi macfad.) and six sweet orange [citrus sinensis (l.) osbeck] orchards from march to august 2006 when the pest is more abundant in southern texas. although flush shoot infestation levels did not vary with host plant species, densities of d. citri eggs, nymphs, and adults were significantly higher on sweet orange than on grapefruit. d. citri immatures also were fou ... | 2008 | 18767763 |
| cspldalpha1 and cspldgamma1 are differentially induced during leaf and fruit abscission and diurnally regulated in citrus sinensis. | understanding leaf and fruit abscission is essential in order to develop strategies for controlling the process in fruit crops. mechanisms involved in signalling leaf and fruit abscission upon induction by abscission agents were investigated in citrus sinensis cv. 'valencia'. previous studies have suggested a role for phospholipid signalling; hence, two phospholipase d cdna sequences, cspldalpha1 and cspldgamma1, were isolated and their role was examined. cspldalpha1 expression was reduced in le ... | 2008 | 18799715 |
| preference and performance of phyllocnistis citrella stainton (lepidoptera: gracillariidae) on three citrus hosts: laboratory and field assessment. | the relationship between preference and performance is crucial to the ecology and evolution of plant-insect interactions. oviposition preference and offspring performance were evaluated for a citrus pest, the leafminer phyllocnistis citrella (lepidoptera: gracillariidae), on three of its host plants: lemon (citrus limon l. burm.), orange (citrus sinensis l. osbeck), and grapefruit (citrus paradisi macfadyen) in tucumán province (northwest argentina). choice and no-choice tests were performed in ... | 2008 | 18801269 |
| antidiabetic potential of citrus sinensis and punica granatum peel extracts in alloxan treated male mice. | an investigation on the effects of four different concentrations of peel extract from citrus sinensis (cs) or punica granatum (pg) in male mice revealed the maximum glucose lowering and antiperoxidative activities at 25 mg/kg of cs and 200 mg/kg of pg. in a separate experiment their potential was evaluated with respect to the regulation of alloxan induced diabetes mellitus. while a single dose of alloxan (120 mg/kg) increased the serum levels of glucose and alpha-amylase activity, rate of water ... | 2007 | 18806305 |
| faunistic analysis of sharpshooters (hemiptera: auchenorrhyncha, cicadellidae) in a 'westin' sweet orange orchard. | the purpose of this study was to analyze sharpshooter fauna in a five-year-old 'westin' sweet orange orchard. yellow sticky traps were placed on the edge of a forest, and on the periphery and inside the citrus stand. the traps were evaluated fortnightly, for three years. the most frequent species were acrogonia citrina marucci & cavichioli, bucephalogonia xanthophis (berg), and oncometopia facialis (signoret). b. xanthophis occurred more in the forest edge, especially on spring and winter. a. ci ... | 2008 | 18813748 |
| the phytochemical analysis and antioxidant activity assessment of orange peel (citrus sinensis) cultivated in greece-crete indicates a new commercial source of hesperidin. | the flavonoid content of several methanolic extract fractions of navel orange peel (flavedo and albedo of citrus sinensis) cultivated in crete (greece) was first analysed phytochemically and then assessed for its antioxidant activity in vitro. the chemical structures of the constituents fractionated were originally determined by comparing their retention times and the obtained uv spectral data with the available bibliographic data and further verified by detailed lc-dad-ms (esi+) analysis. the m ... | 2009 | 18823075 |
| [study on the solubilization of o/w microemulsion for volatile oil]. | to study the solubilization of o/w microemulsion system for volatile oil. | 2008 | 18831200 |
| disease development and symptom expression of xanthomonas axonopodis pv. citri in various citrus plant tissues. | abstract experimental inoculations of xanthomonas axonopodis pv. citri in different tissues of tahiti lime and pineapple sweet orange were conducted monthly under natural conditions on réunion island. the interactions between a set of environmental and epidemic variables associated with disease expression and 184 different factor combinations were investigated to determine the parameters needed to explain asiatic citrus canker (acc) disease expression. area under the disease progress curve (audp ... | 2003 | 18943164 |
| pathogenic interactions between xanthomonas axonopodis pv. citri and cultivars of pummelo (citrus grandis). | abstract the aggressiveness of strains of xanthomonas axonopodis pv. citri on seven citrus species, including citrus sinensis (navel orange), c. paradisi (grapefruit), c. unshiu (satsuma mandarin), c. junos (yuzu), c. aurantifolia ('mexican' lime), c. tachibana (tachibana), and c. grandis (pummelo: 'otachibana', 'banpeiyu', and 'anseikan'), were assessed by comparing lesion expansion and growth in planta, using a prick inoculation method. the existence of two groups distinct in aggressiveness wa ... | 2000 | 18943380 |
| citrus viroid v: occurrence, host range, diagnosis, and identification of new variants. | the recently described citrus viroid v (cvd-v) has been proposed as a new species of the genus apscaviroid within the family pospiviroidae. analysis of 64 samples from different citrus-growing areas has shown that cvd-v is present in the united states, spain, nepal, and the sultanate of oman. cvd-v found in six sweet orange sources from the sultanate of oman was identical to the reference cvd-v variant, whereas three new variants with sequence identities of 98.6% (cvd-vca), 97.3% (cvd-vst), and ... | 2008 | 18943408 |
| proteomic analysis reveals suppression of bark chitinases and proteinase inhibitors in citrus plants affected by the citrus sudden death disease. | citrus sudden death (csd) is a disease of unknown etiology that greatly affects sweet oranges grafted on rangpur lime rootstock, the most important rootstock in brazilian citriculture. we performed a proteomic analysis to generate information related to this plant pathogen interaction. protein profiles from healthy, csd-affected and csd-tolerant stem barks, were generated using two-dimensional gel electrophoresis. the protein spots were well distributed over a pi range of 3.26 to 9.97 and a mole ... | 2008 | 18943454 |
| inheritance and heritability of resistance to citrus leprosis. | abstract the genetic inheritance of resistance to leprosis, the most important viral disease of citrus in brazil, was characterized through the phenotypic assessment of 143 hybrids resulting from crosses between tangor 'murcott' (citrus sinensis x c. reticulata) and sweet orange 'pêra' (c. sinensis), considered to be resistant and susceptible to the disease, respectively. all plants were grafted onto rangpur lime (c. limonia) and inoculated with citrus leprosis virus, cytoplasmic type through th ... | 2006 | 18943497 |
| protection of citrus rootstocks against phytophthora spp. with a hypovirulent isolate of phytophthora nicotianae. | abstract phytophthora root rot of citrus in florida is caused by phytophthora nicotianae and p. palmivora. a naturally occurring isolate of p. nicotianae (pn117) was characterized as hypovirulent on citrus roots. pn117 infected and colonized fibrous roots, but caused significantly less disease than the virulent isolates p. nicotianae pn198 and p. palmivora pp99. coincident inoculation of rootstock seedlings of cleopatra mandarin (citrus reticulata) or swingle citrumelo (c. paradisi x poncirus tr ... | 2007 | 18943635 |
| genetic diversity of xylella fastidiosa strains from costa rica, são paulo, brazil, and united states. | abstract the diversity of 42 xylella fastidiosa strains from costa rica, são paulo, brazil, and the united states were analyzed using the sequence of the 16s rrna gene by variable number of tandem repeat (vntr) fragment analysis and by restriction fragment length polymorphisms (rflp) of a specific polymerase chain reaction (pcr)-amplification product using enzyme cfoi. limited variability in the sequence of the 16s rrna gene was observed and, although the separation was not absolute, most strain ... | 2007 | 18943693 |
| a phytoplasma closely related to the pigeon pea witches'-broom phytoplasma (16sr ix) is associated with citrus huanglongbing symptoms in the state of são paulo, brazil. | in february 2007, sweet orange trees with characteristic symptoms of huanglongbing (hlb) were encountered in a region of são paulo state (sps) hitherto free of hlb. these trees tested negative for the three liberibacter species associated with hlb. a polymerase chain reaction (pcr) product from symptomatic fruit columella dna amplifications with universal primers fd1/rp1 was cloned and sequenced. the corresponding agent was found to have highest 16s rdna sequence identity (99%) with the pigeon p ... | 2008 | 18943735 |
| presence of xylella fastidiosa in sweet orange fruit and seeds and its transmission to seedlings. | abstract xylella fastidiosa, a xylem-limited bacterium, causes several economically important diseases in north, central, and south america. these diseases are transmitted by sharpshooter insects, contaminated budwood, and natural root-grafts. x. fastidiosa extensively colonizes the xylem vessels of susceptible plants. citrus fruit have a well-developed vascular system, which is continuous with the vascular system of the plant. citrus seeds develop very prominent vascular bundles, which are atta ... | 2003 | 18943861 |
| an evaluation of the genetic diversity of xylella fastidiosa isolated from diseased citrus and coffee in são paulo, brazil. | abstract strains of xylella fastidiosa, isolated from sweet orange trees (citrus sinensis) and coffee trees (coffea arabica) with symptoms of citrus variegated chlorosis and requeima do café, respectively, were indistinguishable based on repetitive extragenic palindromic polymerase chain reaction (pcr) and enterobacterial repetitive intergenic consensus pcr assays. these strains were also indistinguishable with a previously described pcr assay that distinguished the citrus strains from all other ... | 2001 | 18943950 |
| postbloom fruit drop of citrus and key lime anthracnose are caused by distinct phylogenetic lineages of colletotrichum acutatum. | colletotrichum acutatum causes two diseases of citrus, postbloom fruit drop (pfd) and key lime anthracnose (kla). pfd is a disease restricted to flowers of sweet orange and most other citrus, and symptoms include petal necrosis, abscission of developing fruit, and the formation of persistent calyces. kla is a disease of foliage, flowers, and fruits of key lime only, and symptoms include necrotic lesions on leaves, fruits, twigs, flowers, and blight of entire shoots. the internal transcribed spac ... | 2008 | 18944086 |
| geographical genetic structure of xylella fastidiosa from citrus in são paulo state, brazil. | abstract a total of 360 xylella fastidiosa strains were isolated from sweet orange (citrus sinensis) cv. pera plants growing in five geographic regions in the brazilian state of são paulo. the genetic variation of these strains was determined by 15 variable number tandem repeat (vntr) and 58 random amplified polymorphic dna (rapd) markers. the mean values of genetic diversity (h) of x. fastidiosa strains within each geographic region determined by rapd (h(rapd)) were substantially lower than h(v ... | 2003 | 18944153 |
| quantification of xylella fastidiosa from citrus trees by real-time polymerase chain reaction assay. | abstract xylella fastidiosa is the causal agent of citrus variegated chlorosis (cvc), a destructive disease of sweet orange cultivars in brazil. polymerase chain reaction (pcr)-based assays constitute the principal diagnostic method for detection of these bacteria. in this work, we established a real-time quantitative pcr (qpcr) assay to quantify x. fastidiosa in naturally and artificially infected citrus. the x. fastidiosa cell number detected in the leaves increased according to the age of the ... | 2002 | 18944214 |
| spatial and temporal analyses of citrus sudden death as a tool to generate hypotheses concerning its etiology. | abstract citrus sudden death (csd), a new disease of unknown etiology that affects sweet orange grafted on rangpur lime, was visually monitored for 14 months in 41 groves in brazil. ordinary runs analysis of csd-symptomatic trees indicated a departure from randomness of symptomatic trees status among immediately adjacent trees mainly within rows. the binomial index of dispersion (d) and the intraclass correlation (k) for various quadrat sizes suggested aggregation of csd-symptomatic trees for al ... | 2003 | 18944366 |
| mineral content and botanical origin of spanish honeys. | eleven elements (zn, p, b, mn, mg, cu, ca, ba, sr, na and k) were determined by inductively plasma coupled spectrometry in 40 honey samples from different places of spain and four different botanical origins: eucalyptus (eucalyptus sp.), heather (erica sp.), orange-blossom (citrus sinensis) and rosemary (rosmarinus officinalis). k, ca and p show the higher levels with average concentrations ranged between 434.1-1935mgkg(-1) for k; 42.59-341.0mgkg(-1) for ca and 51.17-154.3mgkg(-1) for p. levels ... | 2005 | 18969853 |
| proteomic analysis of somatic embryogenesis in valencia sweet orange (citrus sinensis osbeck). | two dimensional gel electrophoresis combined with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms) was employed to study the somatic embryogenesis (se) in valencia sweet orange (citrus sinensis osbeck). twenty-four differentially expressed proteins were identified at five time points of citrus se (0, 1, 2, 3, 4 weeks after embryo initiation) covering globular, heart/torpedo and cotyledon-shaped embryo stages. the general expression patterns for these pr ... | 2009 | 18989674 |
| cloning, characterization and localization of chs gene from blood orange, citrus sinensis (l.) osbeck cv. ruby. | chalcone synthase (chs) is involved in the biosynthesis of anthocyanin. in this study, a full-length dna of chs gene (named as cschs-bo) was cloned from the blood orange, citrus sinensis (l.) osbeck cv. ruby. the gene was 1,512 bp in size containing an open reading frame (1,176 bp) encoding 391 amino acids. comparative and bioinformatic analyses revealed that the deduced protein of cschs-bo was highly homologous to chs from other plant species. the protein of cschs-bo had four chs-specific conse ... | 2009 | 19005777 |
| transcriptional analysis of the sweet orange interaction with the citrus canker pathogens xanthomonas axonopodis pv. citri and xanthomonas axonopodis pv. aurantifolii. | xanthomonas axonopodis pv. citri (xac) and xanthomonas axonopodis pv. aurantifolii pathotype c (xaa) are responsible for citrus canker disease; however, while xac causes canker on all citrus varieties, xaa is restricted to mexican lime, and in sweet oranges it triggers a defence response. to gain insights into the differential pathogenicity exhibited by xac and xaa and to survey the early molecular events leading to canker development, a detailed transcriptional analysis of sweet orange plants i ... | 2008 | 19018992 |
| ammonium transport and citamt1 expression are regulated by n in citrus plants. | citrus seedlings (citrus sinensis l. osbeck x poncirus trifoliata blanco) were used to describe the effects of different n treatments on the nh4+ influx mediated by high- and low-affinity transport systems (hats and lats, respectively) and citamt1 gene expression. results show that citrus plants favor nh4+ over no3- influx mediated by hats and lats when both n sources are present in the nutrient solution and citrus plants display a much higher capacity to take up nh4+ than no3-. furthermore, nh4 ... | 2009 | 19023591 |
| high-efficiency agrobacterium-mediated transformation of citrus via sonication and vacuum infiltration. | an improved method for the agrobacterium infiltration of epicotyl segments of 'pineapple' sweet orange [citrus sinensis (l.) osbeck] and 'swingle' citrumelo [citrus paradisi macf. x poncirus trifoliata (l.) raf.] was developed in order to increase transformation frequency. sonication-assisted agrobacterium-mediated transformation (saat), vacuum infiltration, and a combination of the two procedures were compared with conventional agrobacterium-mediated inoculation method ('dipping' method). it wa ... | 2009 | 19048258 |
| evaluation of larvicidal and nymphicidal potential of plant extracts against anopheles subpictus grassi, culex tritaeniorhynchus giles and aphis gossypii glover. | the acetone, chloroform, ethyl acetate, hexane and methanol extracts of peel and leaf extracts of citrus sinensis, ocimum canum, ocimum sanctum and rhinacanthus nasutus were tested against fourth instar larvae of malaria vector, anopheles subpictus grassi, japanese encephalitis vector, culex tritaeniorhynchus giles (diptera: culicidae) and feeding deterrence to nymphs of cotton pest, aphis gossypii glover (homoptera: aphididae). the larval and nymph mortality were observed after 24 h of exposure ... | 2009 | 19050919 |
| response of sweet orange (citrus sinensis) to 'candidatus liberibacter asiaticus' infection: microscopy and microarray analyses. | citrus greening or huanglongbing (hlb) is a devastating disease of citrus. hlb is associated with the phloem-limited fastidious prokaryotic alpha-proteobacterium 'candidatus liberibacter spp.' in this report, we used sweet orange (citrus sinensis) leaf tissue infected with 'ca. liberibacter asiaticus' and compared this with healthy controls. investigation of the host response was examined with citrus microarray hybridization based on 33,879 expressed sequence tag sequences from several citrus sp ... | 2009 | 19055434 |
| monodemethylated polymethoxyflavones from sweet orange (citrus sinensis) peel inhibit growth of human lung cancer cells by apoptosis. | polymethoxyflavones (pmfs) are almost exclusively found in the citrus genus, particularly in the peels of sweet orange (citrus sinensis l. osbeck) and mandarin (c. reticulate blanco). we studied the effects of two major pmfs, namely, nobiletin and 3,5,6,7,8,3',4'-heptamethoxyflavone (hmf), and two major monodemethylated pmfs, namely 5-hydroxy-3,7,8,3',4'-pentamethoxyflavone (5hpmf), and 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone (5hhmf), on the growth of human lung cancer h1299, h441, and h460 c ... | 2009 | 19065586 |
| light, scanning electron microscopy and sds-page studies on the effect of the essential oil, citrus sinensis var. balady on the embryonic development of camel tick hyalomma dromedarii (koch, 1818) (acari: ixodidae). | gc-mse analysis of the essential oil of fresh fruit peel of citrus sinensis var. balady recognized two main natural toxic compounds, limonene (83.28%) as hydrocarbon compound and linalool (3.97%) as oxygenated compound. therefore, the objective of this study was to evaluate its effect on different egg-ages of hyalomma dromedarii at four concentrations of 1:40, 1:30, 1:20 and 1:15 (oil : ethanol 95%) (v/v). the lc50 values were 1:56, 1:34, 1:41, 1:32, 1: 23, 1:23, 1:18, 1:14 and 1:11 for egg-ages ... | 2007 | 19069909 |
| campylobacter and arcobacter species sensitivity to commercial orange oil fractions. | seven orange oil fractions were screened for their ability to inhibit the growth of selected campylobacter and arcobacter spp. using the standard agar-disk diffusion assay. cold pressed (cp) terpeneless valencia orange oil was found to be the most inhibitory to both campylobacter jejuni and campylobacter coli, exhibiting maximum zones of inhibition up to 80+/-0.0 mm. five-fold concentrated valencia oil and distilled d-limonene resulted in campylobacter inhibition zones ranging from 11.0+/-1.4 to ... | 2009 | 19070381 |
| effect of foliar application of micronutrients on the yield and quality of sweet orange (citrus sinensis l.). | an experiment was designed to study the effect of foliar application of micronutrients on the yield, quality and leaf composition of sweet orange, blood red variety at shabazgari, mardan. the experiment was laid out in a randomized complete block design in 2) factorial arrangement. zinc, manganese and boron were applied as foliar spray at the rate of 0.4, 0.2 and 0.04 kg ha(-1), respectively in the presence of 1.56 kg n ha(-1) as urea and 0.4 kg surfactance ha(-1) (as wetting agent) in 400 l of ... | 2007 | 19086544 |
| the effects of nitrogen and potassium nutrition on the growth of nonembryogenic and embryogenic tissue of sweet orange (citrus sinensis (l.) osbeck). | mineral nutrients are one of the most basic components of plant tissue culture media. nitrogen in the form of nh4+ and no3(-) is the dominant mineral nutrient in most plant tissue culture formulations, with effects dependent on both the proportion and the amount of nh4+ and no3(-). the effects of nitrogen nutrition on the growth of nonembryogenic and embryogenic cell lines of sweet orange (c. sinensis (l.) osbeck cv. 'valencia'), tissues routinely used in citrus horticultural and plant improveme ... | 2008 | 19087252 |
| cellulase production by trichoderma longi, aspergillus niger and saccharomyces cerevisae cultured on waste materials from orange. | the wastes materials from the sweet orange (citrus sinensis) were used as substrate for the production of cellulase. the rind, the pericarp or albedo and the pulp were hydrolyzed by cellulolytic enzymes of trichoderma longibrachiatum, aspergillus niger and saccharomyces cerevisiae after they were treated with alkali and steam. the amount of glucose released from the substrates following the secretion of cellulase by the three microorganisms was measured. the orange wastes released amounts of glu ... | 2008 | 19137846 |
| hesperidin from citrus sinensis cultivated in dezful, iran. | this study described procedures for extracting and quantitation of hesperidin in the waste orange peel of dezful. two extracting procedure were used. in procedure a hesperidin was isolated from orange peel by extracting the dry peel first with petroleum ether, removing the essential oil and then with methanol. in procedure b alkaline extraction followed by acidification of the extract was used. it was purified by treatment with formamide-activated charcoal. detailed analysis of uv, ir, 1hnmr, 13 ... | 2008 | 19137859 |
| the effect of pre spring spray to reduce of citrus important pests. | the importance of pre spring spray against citrus aphids, pulvinaria aurantii cockerell and panonychus citri mcgregor that are the most important pest of citrus during spring was tested. in this research, 150 trees ten years old sweet orange (thomson navel on citrus aurantium (root stocks)) in a citrus orchard approximately three hectares sampled. the experiment was laid out in a totally randomized (one-way) design replicated five times. according to the results, the pre spring spray do not effe ... | 2008 | 19137868 |
| effect of citrus paradisi extract and juice on arterial pressure both in vitro and in vivo. | citrus paradisi (grapefruit) consumption is considered as beneficial and it is popularly used for the treatment of a vast array of diseases, including hypertension. in the present study, the coronary vasodilator and hypotensive effects of citrus paradisi peel extract were assessed in the langendorff isolated and perfused heart model and in the heart and lung dog preparation. in both models, citrus paradisi peel extract decreased coronary vascular resistance and mean arterial pressure when compar ... | 2009 | 19153985 |
| quantitative distribution of 'candidatus liberibacter asiaticus' in citrus plants with citrus huanglongbing. | citrus huanglongbing (hlb), or greening disease, is strongly associated with any of three nonculturable gram-negative bacteria belonging to 'candidatus liberibacter spp.' 'ca. liberibacter spp.' are transmitted by citrus psyllids to all commercial cultivars of citrus. the diseases can be lethal to citrus and have recently become widespread in both são paulo, brazil, and florida, united states, the locations of the largest citrus industries in the world. asiatic hlb, the form of the disease found ... | 2009 | 19159305 |
| nuclear magnetic resonance characterization of metabolite disorder in orange trees caused by citrus sudden death disease. | citrus sudden death (csd) is a new disease of sweet orange and mandarin trees grafted on rangpur lime and citrus volkameriana rootstocks. it was first seen in brazil in 1999, and has since been detected in more than four million trees. the csd causal agent is unknown and the current hypothesis involves a virus similar to citrus tristeza virus or a new virus named citrus sudden death-associated virus. csd symptoms include generalized foliar discoloration, defoliation and root death, and, in most ... | 2009 | 19161352 |
| a novel hybridization approach for detection of citrus viroids. | citrus plants are natural hosts of several viroid species all belonging to the family pospiviroidae. previous attempts to detect viroids from field-grown species and cultivars yielded erratic results unless analyses were performed using etrog citron a secondary bio-amplification host. to overcome the use of etrog citron a number of rt-pcr approaches have been proposed with different degrees of success. here we report the suitability of an easy to handle northern hybridization protocol for viroid ... | 2009 | 19162174 |
| the mechanism of action of a citrus oil blend against enterococcus faecium and enterococcus faecalis. | the aim was to explore the mechanisms by which a blend of orange (citrus sinensis) : bergamot (citrus bergamia) (1 : 1 v/v) eo (essential oil) (2% v/v) and its vapour (15 mg l(-1) air) brings about its antimicrobial effect against enterococcus faecium and enterococcus faecalis. | 2009 | 19187138 |
| estimation of transgene copy number in transformed citrus plants by quantitative multiplex real-time pcr. | quantitative real-time pcr (qrt-pcr) was adapted to estimate transgene copy number of the rice xa21 gene in transgenic citrus plants. this system used taqman qrt-pcr and the endogenous citrus gene encoding for lipid transfer protein (ltp). transgenic "hamlin" sweet orange plants were generated using two different protoplast-gfp transformation systems: cotransformation and single plasmid transformation. a dilution series of genomic dna from one of the transgenic lines was used to generate a stand ... | 2015 | 19194937 |
| effect of surface roughness on retention and removal of escherichia coli o157:h7 on surfaces of selected fruits. | this study was undertaken to evaluate the effect of surface roughness on the attachment and removal of escherichia coli o157:h7 on selected fruit and metal surfaces. a new method to determine surface roughness was developed using confocal laser scanning microscopy (clsm). a series of 2-d layered images were taken by clsm optical slicing of the surfaces of golden delicious apples, navel oranges, avocadoes, and cantaloupes. the average roughness (r(a)) of the fruit surfaces was assessed by reconst ... | 2009 | 19200095 |
| discrimination between mild and severe citrus tristeza virus isolates with a rapid and highly specific real-time reverse transcription-polymerase chain reaction method using taqman lna probes. | severe isolates of citrus tristeza virus (ctv) inducing seedling yellows (sy) and/or stem pitting (sp) in grapefruit or sweet orange are a major threat for the citrus industry worldwide. identification of these ctv variants was achieved by quantitative real-time reverse transcription-polymerase chain reaction (rt-pcr) using a general primer set and three taqman locked nucleic acids (lna) probes targeting sequences characteristic of severe, mild (non-sy, non-sp), and t36-like isolates. successful ... | 2009 | 19203284 |
| characterization of gibberellin 20-oxidases in the citrus hybrid carrizo citrange. | ccga20ox1 is a gene encoding a ga 20-oxidase, a gibberellin (ga) biosynthetic enzyme, previously isolated from the citrus hybrid carrizo citrange (citrus sinensis (l.) osbeck x poncirus trifoliata (l.) raf.). southern blot analysis of genomic dna of carrizo citrange with ccga20ox1 suggested the presence in the hybrid of another gene encoding another ga 20-oxidase. a cdna clone from this new gene (ccga20ox2) was isolated using rna from the other parent c. sinensis. ccga20ox2 encoded a protein of ... | 2009 | 19203975 |
| transcriptome analysis of a spontaneous mutant in sweet orange [citrus sinensis (l.) osbeck] during fruit development. | bud mutations often arise in citrus. the selection of mutants is one of the most important breeding channels in citrus. however, the molecular basis of bud mutation has rarely been studied. to identify differentially expressed genes in a spontaneous sweet orange [c. sinensis (l.) osbeck] bud mutation which causes lycopene accumulation, low citric acid, and high sucrose in fruit, suppression subtractive hybridization and microarray analysis were performed to decipher this bud mutation during frui ... | 2009 | 19218315 |
| pharmacological exploration of the sedative mechanism of hesperidin identified as the active principle of citrus sinensis flowers. | the infusion of flowers of several species of citrus genera is used as a sedative to treat insomnia in mexican traditional medicine. the aims of this study were to investigate the sedative effect of different extracts of flowers of citrus sinensis (l.) osbeck (rutaceae) and describe the pharmacological action mechanism of the sedative active compounds of this plant. the methanol and dichloromethane extracts, obtained from the flowers of citrus sinensis (l.) osbeck (rutaceae), showed a dose-depen ... | 2009 | 19219759 |
| influence of spray equipment and water volume on coverage of citrus and control of citricola scale, coccus pseudomagnoliarum (hemiptera: coccidae) with mineral oil. | a trial was conducted in a commercial citrus sinensis l. variety 'washington' navel orange orchard to compare the coverage and efficacy against citricola scale coccus pseudomagnoliarum (kuwana) (hemiptera: coccidae) of 45.5 liters/ha of an nc24 agricultural mineral oil treatment applied by two different methods: a conventional air blast sprayer and a rotary atomizer. three water volumes (2,340, 4,680, and 7,020 liters/ha) were applied with the air blast sprayer to determine the optimal spray vol ... | 2009 | 19253648 |
| characterization of citrus sinensis type 1 mitochondrial alternative oxidase and expression analysis in biotic stress. | the higher plant mitochondrial electron transport chain contains an alternative pathway that ends with the aox (alternative oxidase). the aox proteins are encoded by a small gene family composed of two discrete gene subfamilies. aox1 is present in both monocot and eudicot plants, whereas aox2 is only present in eudicot plants. we isolated a genomic clone from citrus sinensis containing the aox1a gene. the orange aox1a consists of four exons interrupted by three introns and its promoter harbours ... | 2009 | 19257856 |
| contents of phytosterols in vegetables and fruits commonly consumed in china. | to quantify five specific dietary phytosterols and phytostanols in vegetables and fruits commonly consumed in china. | 2008 | 19263798 |
| responses of tylenchulus semipenetrans to citrus fruit removal: implications for carbohydrate competition. | sixteen mature valencia orange trees on rough lemon rootstock were selected on the basis of approximately equal, naturally occurring populations of tylenchulus semipenetrans in soil. in march, fruit 1 cm in diameter or less were removed from eight of the trees, which were kept free of fruit for 15 months. in july, 4 months after fruit removal, fibrous root (<2 mm d) mass density of defruited trees was 51% greater and insoluble starch in fibrous roots was 24% less than on control trees with fruit ... | 1993 | 19279735 |
| influence of aldicarb and fenamiphos on tylenchulus semipenetrans population densities and orange yield. | the effect of aldicarb and fenamiphos on tylenchulus semipenetrans population densities and on orange yield was investigated during a 3-year (1986-88) field trial in italy. rates were 10 and 20 kg a.i./ha as an early spring single application, 5 kg a.i./ha in spring and 5 kg after flowering, and 5 kg a.i. in spring followed by 2.5 kg/ha after flowering and 2.5 kg/ha in early autumn. rates and times of application of the two nematicides did not affect numbers of females of t. semipenetrans on the ... | 1993 | 19279837 |
| spiroplasmas are the causal agents of citrus little-leaf disease. | a spiroplasma isolated from citrus with little-leaf disease was grown in a cell-free medium and injected into leafhoppers (euscelis plebejus). injected leafhoppers, but not those fed on infected plants, transmitted the spiroplasma to white clover (trifolium repens cv. s100) and sweet orange (citrus sinensis cv. valencia). infected clover plants were severely stunted; infected sweet orange plants showed typical symptoms of citrus little-leaf disease. the spiroplasma was detected in clover and swe ... | 1974 | 19280788 |
| xiphidorus amazonensis n. sp. (nematoda: longidoridae) from the brazilian amazon basin. | xiphidorus amazonensis n. sp. was found in the rhizospheres of jatropha curcas, musa sp., anona muricata, cassia tora, panicum laxum, paspalum fasciculatum, aeschynomene sensitiva, saccharum officinarum, manihot esculenta, abelmoschus esculentus, tamarindus indica, mangifera indica, vigna unguiculata, zea mays, commelina sp., cyperus rotundus, fimbristylis miliacea, citrus sinensis, and eichhornia crassipes on the amazon river island of xiborena, approximately 40 km southeast of manaus, capital ... | 1985 | 19294098 |
| variation in radopholus citrophilus population densities in the citrus rootstock carrizo citrange. | seedlings of the hybrid citrus rootstock, carrizo citrange (citrus sinensis x poncirus trifoliata) do not uniformly limit development of the citrus burrowing nematode, radopholus citrophilus. variation in nematode population densities in roots of seedlings germinating from the same seed suggests that factors responsible for nematode incompatibility are not functional or are not inherited uniformly among progeny. seeds which produced a single seedling were more likely to produce plants which supp ... | 1986 | 19294135 |
| isolation of xanthyletin, an inhibitor of ants' symbiotic fungus, by high-speed counter-current chromatography. | xanthyletin, an inhibitor of symbiotic fungus (leucoagaricus gongylophorus) of leaf-cutting ant (atta sexdens rubropilosa), as well as suberosin, seselin and xanthoxyletin were isolated from citrus sinensis grafted on citrus limonia. a two-phase solvent system composed of hexane/ethanol/acetonitrile/water (10:8:1:1, v/v) was used for the high-speed counter-current chromatographic isolation of xanthyletin with high yield and over 99% purity as determined by liquid and gas chromatography with mass ... | 2009 | 19296958 |
| effects of oxamyl on the citrus nematode, tylenchulus semipenetrans, and on infection of sweet orange. | foliar sprays of 4 microg/ml oxamyl on sweet orange trees in a greenhouse slightly depressed the number of tylenchulus semipenetrans larvae obtained from roots and soil, but similar treatments were not effective in two orchards. soil drench treatments decreased the number of citrus nematode larvae obtained from roots or soil of citrus plants grown itt a greenhouse and in orchards. exposure to 5-10 microg/ml of oxamyl in water was lethal to only a few second-stage larvae treated 10 days, and many ... | 1976 | 19308209 |
| the effect of soil type on movement and infection rate of larvae of tylenchulus semipenetrans. | most of the tylenchulus semipenetrans larvae applied on the surface of four soils in pots 14.5-cm deep moved no further downward than 6.5 crn, and remained in the upper half of the pot. the percentage of second-stage larvae that developed into adult females on 'homosassa' sweet orange in the soils were: sandy loam, 6.8% in the same soil with inoculation holes, 8.6%; loamy sand, 5.4%; coarse sand, 0.2%; and in sand-peat (2:1, v/v)mixture 0.04%. low percentage infection in coarse sand and sand-pea ... | 1974 | 19319368 |
| four biotypes of tylenchulus semipenetrans in california identified, and their importance in the development of resistant citrus rootstocks. | four biotypes (pathotypes) of the citrus nematode, tylenchulus semipenetrans, occurring in california, u.s.a. were differentiated on the basis of differences of infectivity on 'homosassa' sweet orange, 'troyer' citrange, 'pomeroy' and 'rubidoux' poncirus trifoliata, 'thompson seedless' grape, and 'manzanillo' olive. a method for differentiating biotypes of t. semipenetrans is described. field observations indicate that biotypes of this nematode are very stable. the importance of using highly inf ... | 1974 | 19319369 |
| in vitro antilisterial effects of citrus oil fractions in combination with organic acids. | the objectives of this study were to screen activity of citrus essential oil fractions (eos) alone and in combination with organic acids against 2 species of listeria. five citrus eos were initially screened by disc diffusion assay for antibacterial activity. cold pressed terpeneless valencia orange oil (cp terpeneless oil) had the strongest bacteriostatic (mic) and bactericidal (mbc) properties at 0.55% and 1.67%, respectively. four organic acids were tested for effectiveness against listeria. ... | 2009 | 19323760 |
| molecular and functional characterization of a novel chromoplast-specific lycopene beta-cyclase from citrus and its relation to lycopene accumulation. | carotenoids are the main pigments responsible of the colouration of citrus fruits. the beta-cyclization of lycopene, catalysed by the lycopene beta-cyclases (beta-lcy), seems to be a key regulatory step of the carotenoid pathway. in the present study, two beta-lcys from orange fruits (citrus sinensis), named csbeta-lcy1 and csbeta-lcy2 have been isolated and the activity of the encoded proteins was demonstrated by functional analysis. csbeta-lcy1 was expressed at low levels and remained relative ... | 2009 | 19325166 |
| infectivity of two biotypes of the citrus nematode on citrus and on some other hosts. | the infectivity and development of two biotypes of citrus nematode (tylenchulus semipenetrans) were compared on highly resistant poncirus trifoliata selection 'pomeroy,' moderately susceptible 'troyer' citrange, and highly susceptible sweet orange selection 'homosassa' small seedlings in a glasshouse. biotype-1 was more infective on the above hosts and developed faster on sweet orange and on 'troyer' citrange than biotype-2. the differences in infectivity were interpreted to reflect differences ... | 1969 | 19325669 |
| host range and genetic relatedness of colletotrichum acutatum isolates from fruit crops and leatherleaf fern in florida. | isolates of colletotrichum acutatum were collected from anthracnose-affected strawberry, leatherleaf fern, and key lime; ripe-rot-affected blueberry; and postbloom fruit drop (pfd)-affected sweet orange in florida. additional isolates from ripe-rot-affected blueberry were collected from georgia and north carolina and from anthracnose-affected leatherleaf fern in costa rica. pathogenicity tests on blueberry and strawberry fruit; foliage of key lime, leatherleaf fern, and strawberry; and citrus fl ... | 2009 | 19351258 |
| radical scavenging activity of orange and tangerine varieties cultivated in brazil. | four citrus fruit varieties cultivated in brazil (two kinds of sweet orange and two kinds of tangerine) were analyzed for physicochemical characteristics contents of total phenolics, total carotenoids and ascorbic acid, and antioxidant activities. the antioxidant activities of aqueous, methanolic, and acetone extracts of the citrus fruit juices were assessed on the basis of their ability to scavenge 1,1-diphenyl-2-picrylhydrazyl (dpph( )). the cravo tangerine has the highest content of citric ac ... | 2009 | 19381992 |
| gene isolation and expression analysis of two distinct sweet orange [citrus sinensis l. (osbeck)] tau-type glutathione transferases. | glutathione s-transferases (gsts) represent a multifunctional family of enzymes grouped into four main classes (tau, phi, theta, and zeta) conjugating endobiotic and xenobiotic compounds to glutathione. in plants, this is considered to be a crucial step in the detoxification process as the s-glutathionylated metabolites are tagged for vacuolar sequestration. in this work, we have isolated two glutathione s-transferases belonging to the tau class gsts from sweet orange leaves. the cdna clones con ... | 2009 | 19422890 |