Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| qtl analysis of citrus tristeza virus-citradia interaction. | citrus tristeza virus (ctv) has caused the death of millions of trees grafted on sour orange ( citrus aurantium). however, this rootstock is very well adapted to the mediterranean, semi-arid conditions. the aim of the present research is to genetically analyze the accumulation of ctv in a progeny derived from the cross between c. aurantium and poncirus trifoliata, both resistant to ctv isolate t-346. graft propagation of 104 hybrids was done on healthy sweet orange as a rootstock. three months l ... | 2004 | 14614564 |
| expressed sequence enrichment for candidate gene analysis of citrus tristeza virus resistance. | several studies have reported markers linked to a putative resistance gene from poncirus trifoliata ( ctv-r) located at linkage group 4 that confers resistance against one of the most important citrus pathogens, citrus tristeza virus (ctv). to be successful in both marker-assisted selection and transformation experiments, its accurate mapping is needed. several factors may affect its localization, among them two are considered here: the definition of resistance and the genetic background of prog ... | 2004 | 14624336 |
| the dwarfing mechanism of citrus rootstocks f&a 418 and #23 is related to competition between vegetative and reproductive growth. | the annual development of navelina (citrus sinensis (l.) osbeck) trees budded on three hybrid citrus rootstocks was studied. two rootstocks, named #23 and #24, were obtained from the cross of troyer citrange (c. sinensis x poncirus trifoliata (l.) raf.) x cleopatra mandarin (c. reshni hort. ex tan.). the third rootstock, named f&a 418, came from a cross of troyer citrange x common mandarin (c. deliciosa ten.). rootstocks #23 and f&a 418 are dwarfing rootstocks and reduce the size of the scion by ... | 2004 | 14676038 |
| effect of nigerian citrus (citrus sinensis osbeck) honey on ethanol metabolism. | the effect of nigerian citrus (citrus sinensis osbeck) honey on ethanol metabolism was tested using 45 consenting individuals in apparent good health and between the ages of 25 and 35 years. the subjects were moderate social drinkers matched in terms of body weight and build. the results obtained showed that on average, honey significantly (p < 0.05) increased the blood ethanol clearance rate by 68% and decreased the intoxication period by 43%, but insignificantly (p > 0.05) reduced the degree o ... | 2004 | 15662998 |
| characterization of anthocyanins and pyranoanthocyanins from blood orange [citrus sinensis (l.) osbeck] juice. | anthocyanins from blood orange [citrus sinensis (l.) osbeck] juices were isolated and purified by means of high-speed countercurrent chromatography and preparative high-performance liquid chromatography. structures of the pigments were then elucidated by electrospray ionization multiple mass spectrometry and nuclear magnetic resonance (nmr) spectroscopy. the major anthocyanins of the juice were characterized as cyanidin 3-glucoside and cyanidin 3-(6"-malonylglucoside). furthermore, six minor ant ... | 2004 | 15563216 |
| biochemical and molecular analysis of carotenoid biosynthesis in flavedo of orange (citrus sinensis l.) during fruit development and maturation. | changes in carotenoid content and composition and expression of carotenoid biosynthetic genes were analyzed in the flavedo of sweet orange (citrus sinensis l. osbeck, cv. navelate) fruit during development and maturation. lutein and all-e-violaxanthin were the major carotenoids in chloroplast-containing tissues. during fruit coloration, phytoene, beta-cryptoxanthin, zeaxanthin, and mainly (9z)-violaxanthin progressively accumulated, and a large proportion of apocarotenoids was also found in the ... | 2004 | 15506808 |
| a beta-galactosidase gene is expressed during mature fruit abscission of 'valencia' orange (citrus sinensis). | beta-galactosidases have been detected in a wide range of plants and are characterized by their ability to hydrolyse terminal non-reducing beta-d-galactosyl residues from beta-d-galactosides. these enzymes have been detected in a wide range of plant organs and tissues. in a search for differentially expressed genes during the abscission process in citrus, sequences encoding beta-galactosidase were identified. three cdna fragments of a beta-galactosidase gene were isolated from a cdna subtraction ... | 2004 | 15208347 |
| isolation of a dehydrin cdna from orange and grapefruit citrus fruit that is specifically induced by the combination of heat followed by chilling temperatures. | dehydrins (dhns; late embryogenesis abundant d-11) are a family of plant proteins induced in response to environmental stresses such as water stress, salinity and freezing or which occur during the late stages of embryogenesis. previously, it was reported that citrus contains a small gene family encoding a unique class of dehydrins that differs from most other plant dehydrins in various respects, such as having an unusual k-segment similar to that of gymnosperms. in the present study, we identif ... | 2004 | 15032860 |
| application of mri to monitor the process of ripening and decay in citrus treated with chitosan solutions. | chitosan is a biopolymer, which has numerous applications in agriculture and agroindustries. coating fruit and vegetables with chitosan has some positive advantages for the long-term storage of foods, because the film of chitosan provides a kind of an active package, which allows a gradual release of preservatives, thus inhibiting fungal growth and maintaining the external appearance of the fruit for a longer time in this study, two varieties of citrus, fortune mandarins and valencia oranges wer ... | 2004 | 14972402 |
| developing the sterile insect technique for cryptophlebia leucotreta (lepidoptera: tortricidae): influence of radiation dose and release ratio on fruit damage and population growth in field cages. | the effect of radiation dose and different release ratios of treated (t) to untreated (u) cryptophlebia leucotreta (meyrick) (lepidoptera: tortricidae), on the incidence of fruit damage, the competitiveness of the treated males, and population growth was examined inside field cages. navel orange trees were individually enclosed in large nylon mesh cages. newly emerged adult moths treated with either 150 or 200 gy of gamma radiation were released into the cages at ratios of 5t:1u or 10t:1u. the f ... | 2005 | 16539115 |
| action pattern of valencia orange pme de-esterification of high methoxyl pectin and characterization of modified pectins. | valencia pectinmethylesterase (pme) fractions, b-pme, containing 36 and 13 kda protein bands and u-pme, containing a 36 and 27 kda protein bands, were used to de-esterify original pectin (o-pec) from 73% degree of esterification (%de) to 63% (b-pec) and 61% de (u-pec), respectively. most o-pec eluted from ion exchange chromatography at low salt concentration and a smaller component eluted at higher ionic strength. b-pec and u-pec eluted as one broad peak at higher ionic strength. pme modificatio ... | 2005 | 16216228 |
| identification and aroma impact of norisoprenoids in orange juice. | four norisoprenoids, alpha-ionone, beta-ionone, beta-cyclocitral, and beta-damascenone, along with their putative carotenoid precursors, were identified in valencia orange juice using time-intensity gc-o, gc-ms, and photodiode array hplc. alpha-ionone and beta-cyclocitral are reported in orange juice for the first time. gc-o aroma peaks were categorized into seven groups with similar sensory qualities: citrus/minty, metallic/mushroom/geranium, roasted/cooked/meaty/spice, fatty/soapy/green, sulfu ... | 2005 | 15656678 |
| analysis of antioxidant compounds in sweet orange peel by hplc-diode array detection-electrospray ionization mass spectrometry. | hplc-diode array detection-electrospray ionization mass spectrometry was used to determine qualitatively and quantitatively the flavonoid content of several fractions and residues of extracts of greek navel sweet orange peel (citrus sinensis) from the region of southern greece (leonidi-tripoli). the main groups of flavonoids found according to hplc retention times, spectral data and literature references were polymethoxylated flavones, c-glycosylated flavones, o-glycosylated flavones, o-glycosyl ... | 2005 | 15515108 |
| involvement of ethylene biosynthesis and perception in the susceptibility of citrus fruits to penicillium digitatum infection and the accumulation of defence-related mrnas. | citrus fruits infected with the fungus penicillium digitatum substantially increase the production of the plant hormone ethylene. in this study, the regulation of ethylene biosynthesis in citrus sinensis-infected fruits and its putative involvement in an active defence response against p. digitatum infection is examined. ethylene production is demonstrated as being the result of the co-ordinated and differential up-regulation of at least three ethylene biosynthetic genes: acs1, acs2, and aco. bl ... | 2005 | 15983011 |
| [changes in polyamine levels in citrus sinensis osb. cv. valencia callus during somatic embryogenesis]. | somatic embryogenetic capability and changes in polyamine level and their relationship were analyzed using the long-term (8 years) subcultured calli of citrus sinensis osb. cv. valencia as materials. the results showed that endogenous polyamine contents in embryogenic calli were higher than those in non-embryogenic calli, and the embryogenetic capability was positively correlated to the levels of endogenous polyamines. when the calli were transferred to a differentiation medium, the putrescine c ... | 2005 | 15961902 |
| g-protein-coupled alpha2a-adrenoreceptor agonists differentially alter citrus leaf and fruit abscission by affecting expression of acc synthase and acc oxidase. | temporal and spatial expression patterns of genes encoding 1-aminocyclopropane-1-carboxylate (acc) synthase (acs1 and acs2) and acc oxidase (aco), acc concentration, and ethylene production in leaves and fruit of 'valencia' orange (citrus sinensis [l.] osbeck) were examined in relation to differential abscission after treatment with 2-chloroethylphosphonic acid (ethephon) alone or in combination with guanfacine or clonidine, two g-protein-coupled alpha(2a)-adrenoreceptor selective agonists. guan ... | 2005 | 15928018 |
| relationships between nutrient composition of flowers and fruit quality in orange trees grown in calcareous soil. | to determine if flower nutrient composition can be used to predict fruit quality, a field experiment was conducted over three seasons (1996-1999) in a commercial orange orchard (citrus sinensis (l.) osbeck cv. 'valencia late', budded on troyer citrange rootstock) established on a calcareous soil in southern portugal. flowers were collected from 20 trees during full bloom in april and their nutrient composition determined, and fruits were harvested the following march and their quality evaluated. ... | 2005 | 15805096 |
| isolation, characterization, and pectin-modifying properties of a thermally tolerant pectin methylesterase from citrus sinensis var. valencia. | the thermally tolerant pectin methylesterase (tt-pme) was isolated as a monocomponent enzyme from sweet orange fruit (citrus sinensis var. valencia). it was also isolated from flower and vegetative tissue. the apparent molecular weight of fruit tt-pme was 40800 by sds-page and the isoelectric point estimated as pi 9.31 by ief-page. maldi-tof ms identified no tryptic-peptide ions from tt-pme characteristic of previously described citrus pmes. tt-pme did not absolutely require supplemented salt fo ... | 2005 | 15769165 |
| starter substrate specificities of wild-type and mutant polyketide synthases from rutaceae. | chalcone synthases (chss) and acridone synthases (acss) belong to the superfamily of type iii polyketide synthases (pkss) and condense the starter substrate 4-coumaroyl-coa or n-methylanthraniloyl-coa with three malonyl-coas to produce flavonoids and acridone alkaloids, respectively. acss which have been cloned exclusively from ruta graveolens share about 75-85% polypeptide sequence homology with chss from other plant families, while 90% similarity was observed with chss from rutaceae, i.e., r. ... | 2005 | 15680984 |
| metal-binding proteins scanning and determination by combining gel electrophoresis, synchrotron radiation x-ray fluorescence and atomic spectrometry. | in the present work, protein bands from in vitro embriogenic callus (citrus sinensis l. osbeck) were investigated using micro-synchrotron radiation x-ray fluorescence (musr-xrf) after sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) separation. metal-binding protein quantification was done after microwave oven decomposition of gel by synchrotron radiation total reflection x-ray fluorescence (sr-txrf), flame atomic absorption spectrometry (faas) and flame atomic emission spect ... | 2005 | 15680280 |
| anthocyanins accumulation and related gene expression in red orange fruit induced by low temperature storage. | the aim of this work was to study the impact of moderately long storage periods at 4 degrees c upon red orange [citrus sinensis (l.) osbeck] anthocyanins production and the expression of structural genes involved in their biosynthesis such as phehylalanine ammonia lyase (pal), chalcone synthase (chs), dihydroflavonol 4-reductase (dfr), and udp-glucose flavonoid glucosyl transferase (ufgt). our results showed that low temperature-induced anthocyanins accumulation in red orange juice vesicles afte ... | 2005 | 16277406 |
| lycopene-epsilon-cyclase pre-mrna is alternatively spliced in cara cara navel orange (citrus sinensis osbeck). | lycopene-epsilon-cyclase is one of the key enzymes related to alpha-carotene metabolism in plants. a full-length cdna of 1300 bp encoding lycopene-epsilon-cyclase (lyce) was generated from cara cara navel orange, a unique navel orange containing both lycopene and beta-carotene in its pulp, with little or no alpha-carotene. the gene had a 14 bp nucleotides deletion and caused a terminal mutation. dna sequence corresponding to the deletion region revealed that two repeats of 6 bp (aggtgt) were fla ... | 2005 | 16086259 |
| analysis of flavanone-7-o-glycosides in citrus juices by short-end capillary electrochromatography. | the separation of the major flavanone-7-o-glycoside constituents of citrus was carried out by isocratic reversed phase capillary electrochromatography using a 75 microm i.d. silica fused column packed with 5 microm ods silica gel. in comparison to hplc mode, capillary electrochromatography resolution of flavanone glycosides was obtained with a high selectivity factor. optimum separation conditions were found using a mixture of ammonium formate (ph 2.5)--acetonitrile (8:2, v/v) as the mobile phas ... | 2005 | 16013605 |
| mineral content and botanical origin of spanish honeys. | eleven elements (zn, p, b, mn, mg, cu, ca, ba, sr, na and k) were determined by inductively plasma coupled spectrometry in 40 honey samples from different places of spain and four different botanical origins: eucalyptus (eucalyptus sp.), heather (erica sp.), orange-blossom (citrus sinensis) and rosemary (rosmarinus officinalis). k, ca and p show the higher levels with average concentrations ranged between 434.1-1935mgkg(-1) for k; 42.59-341.0mgkg(-1) for ca and 51.17-154.3mgkg(-1) for p. levels ... | 2005 | 18969853 |
| cover crops and natural vegetation mulch effect achieved by mechanical management with lateral rotary mower in weed population dynamics in citrus. | the aim of this study was to obtain information on practical weed management in order to reduce the use of herbicides thereby contributing to the sustainable development of citrus crop. the experiment was carried out under field conditions at the experimental area of the department of vegetal production at the college of agriculture "luiz de queiroz," piracicaba, sp, brazil, during the season 2002-2003. influence of mulches produced by four types of vegetations on the dynamic population of weeds ... | 2005 | 15656179 |
| repellency and toxicity of aromatic plant extracts against the mosquito culex pipiens molestus (diptera: culicidae). | the insecticidal activities of essential oil extracts from leaves, flowers and roots of aromatic plants against fourth-instar larvae of the mosquito culex pipiens molestus forskal were determined. extracts of foeniculum vulgare mill were the most toxic, followed by those of ferula hermonis boiss, citrus sinensis osbeck, pinus pinea l, laurus nobilis l and eucalyptus spp with lc50 values of 24.5, 44.0, 60.0, 75.0, 117.0 and 120.0 mg litre(-1), respectively. combination tests between the lc50 and ... | 2005 | 15662650 |
| inotropic effect of citrus sinensis (l.) osbeck leaf extracts on the guinea pig atrium. | the objective of the present investigation was to determine the contractile effect of crude and acetone leaf extracts of citrus sinensis (l.) osb. on mammalian myocardium. crude leaf extracts have been used in folk medicine to treat neurological disorders. some flavonoids isolated from this plant presented a positive inotropic effect on myocardium. this motivated us to test the extracts on the atria of guinea pigs of both sexes (300-500 g) and surprisingly we observed inotropic depression instea ... | 2005 | 15665997 |
| establishment of a genetically marked insect-derived symbiont in multiple host plants. | alcaligenes xylosoxidans subsp. denitrificans, originally isolated from the cibarial region of the foregut of the glassy-winged sharpshooter (homalodisca coagulata), was transformed using the himar1 transposition system to express egfp. seedlings of six potential host plants were inoculated with transformed bacteria and 2 weeks later samples were taken 5 cm away and analyzed by quantitative real-time pcr using primers designed to amplify the gene insert. the largest colony of 3,591,427 cells/2 c ... | 2005 | 15723145 |
| the climacteric-like behaviour of young, mature and wounded citrus leaves. | although leaves and other vegetative tissues are generally considered as non-climacteric, citrus leaves show a climacteric system ii behaviour after detachment. upon harvest, young, fully expanded 'valencia' orange (citrus sinensis) leaves ( approximately 60-d-old) exhibited two phases of ethylene production. the first phase, up to 6 d after detachment, was characterized by a low and constant ethylene production (system i pathway), associated with a constitutive expression of acc synthase 2 (csa ... | 2005 | 15767320 |
| varietal and interspecific influence on micronutrient contents in citrus from the mediterranean area. | to specify the genotypic variation of mediterranean citrus juices, the contents of carotenoids, flavonoids, and vitamin c were determined by high-performance liquid chromatography. a selection of orange varieties and mandarin species from the mediterranean area (citrus sinensis, citrus deliciosa ten, and citrus clementina hort. ex tan) was evaluated using carotenoid profiles and flavanones contents. among the eight varieties of orange (salustiana, hamlin, shamouti, pera, valencia, maltaise, sang ... | 2005 | 15769147 |
| leaf volatile compounds of six citrus somatic allotetraploid hybrids originating from various combinations of lime, lemon, citron, sweet orange, and grapefruit. | volatile compounds were extracted by a pentane/ether (1:1) mixture from the leaves of six citrus somatic allotetraploid hybrids resulting from various combinations of lime, lemon, citron, sweet orange, and grapefruit. extracts were examined by gas chromatography-mass spectrometry (gc-ms) and compared with those of their respective parents. all hybrids having an acid citrus parent exhibit the same relative contents in hydrocarbons and oxygenated compounds as the acid citrus, while the (grapefruit ... | 2005 | 15769160 |
| citrus huanglongbing in são paulo state, brazil: pcr detection of the 'candidatus' liberibacter species associated with the disease. | symptoms of huanglongbing (hlb), one of the most serious diseases of citrus in asia and africa, have been noticed in march 2004 in the araraquara region of são paulo state, brazil. hlb has not been reported previously from america. the causal hlb bacteria, candidatus liberibacter africanus in africa and candidatus liberibacter asiaticus in asia, can be detected in symptomatic citrus leaves by pcr amplification of their 16s rdna with previously described primers. when this technique was applied t ... | 2005 | 15797817 |
| transcription analysis of pils and xpsel genes from xylella fastidiosa. | xylella fastidiosa is a xylem-limited phytopathogen responsible for diseases in several plants such as citrus and coffee. analysis of the bacterial genome revealed some putative pathogenicity-related genes that could help to elucidate the molecular mechanisms of plant-pathogen interactions. in the present work, the transcription of three genes of the bacterium, grown in defined and rich media and also in media containing host plant extracts (sweet orange, 'ponkan' and coffee) was analyzed by rt- ... | 2005 | 15803391 |
| a set of primers for analyzing chloroplast dna diversity in citrus and related genera. | chloroplast simple sequence repeat (cpssr) markers in citrus were developed and used to analyze chloroplast diversity of citrus and closely related genera. fourteen cpssr primer pairs from the chloroplast genomes of tobacco (nicotiana tabacum l.) and arabidopsis were found useful for analyzing the citrus chloroplast genome (cpdna) and recoded with the prefix spcc (ssr primers for citrus chloroplast). eleven of the 14 primer pairs revealed some degree of polymorphism among 34 genotypes of citrus, ... | 2005 | 15805086 |
| effect of certain bioactive plant extracts on clinical isolates of beta-lactamase producing methicillin resistant staphylococcus aureus. | ethanolic extracts and some fractions from 10 indian medicinal plants, known for antibacterial activity, were investigated for their ability to inhibit clinical isolates of beta-lactamase producing methicillin-resistant staphylococcus aureus (mrsa) and methicillin-sensitive s. aureus (mssa). synergistic interaction of plant extracts with certain antibiotics was also evaluated. the mrsa test strains were found to be multi-drug resistant and also exhibited high level of resistance to common beta-l ... | 2005 | 15812867 |
| protoplast transformation and regeneration of transgenic valencia sweet orange plants containing a juice quality-related pectin methylesterase gene. | valencia orange [citrus sinensis (l.) osbeck] is the leading commercial citrus species in the world for processed juice products; however, the presence of thermostable pectin methylesterase (tspme) reduces its juice quality. a long-term strategy of this work is to eliminate or greatly reduce tspme activity in valencia orange. previous work resulted in the isolation of a putative tspme gene, cspme4, associated with a thermostable protein fraction of valencia orange juice. to begin research design ... | 2005 | 15875190 |
| toward validation of process criteria for high-pressure processing of orange juice with predictive models. | mathematical models were developed to predict time to inactivation (tti) by high-pressure processing of salmonella in australian valencia orange juice (ph 4.3) and navel orange juice (ph 3.7) as a function of pressure magnitude (300 to 600 mpa) and inoculum level (3 to 7 log cfu/ml). for each model, the tti was found to increase with increasing inoculum level and decrease with increasing pressure magnitude. the u.s. food and drug administration juice hazard analysis and critical control point re ... | 2005 | 15895726 |
| antioxidant activity of the flavonoid hesperidin in chemical and biological systems. | the antioxidant hesperidin, a major flavonoid in sweet orange and lemon, was evaluated using chemical and biological systems. the chemical assay evaluates the hesperidin capacity to sequester 1,1-diphenyl-2-picrylhydrazyl (dpph*). biological studies were done using the eukaryotic cells of superoxide-dismutase proficient and deficient strains of saccharomyces cerevisiae treated with hesperidin and the stressing agents hydrogen peroxide or paraquat (methylviologen; 1,1'-dimethyl-4,4'-bipyridinium ... | 2005 | 15941311 |
| [control effects of mikania micrantha alcohol extract on citrus red mite panonychus citri]. | this study showed that spraying 0.2 and 0.4 g x l(-1) mikania micrantha alcohol extracts on panonychus citri could significantly decrease the survival rates of p. citri eggs, larvae and nymphs, and 0.1 g x l(-1) m. micrantha alcohol extract could also significantly decrease the survival rates of larvae and nymphs. in a no-choice test, both the fecundity and the longevity of female p. citri fed on the leaves treated with all three test concentrations 0.1, 0.2 and 0.4 g x l(-1) of m. micrantha alc ... | 2005 | 16011182 |
| [creation and hybrid identification of the sexual progenies using citrus somatic hybrid as pollen parent]. | 110 plantlets were obtained from the cross between citrus allotetraploid somatic hybrid [hamlin sweet orange (citrus sinensis osback) + rough lemon (c. jambhiri luss)] (as the pollen parent) and mono-embryonic diploid type iben no.4 [huanongbendizao tangerine (c. reticulata blanco) x ichang papeda (c. ichangensis swingle)] by the means of young embryo culture in vitro, and there were 93 triploids and 17 diploids through chromosome counting and the ploidy analyser identification. rapd analyses sh ... | 2005 | 16044915 |
| soil water repellency induced by long-term irrigation with treated sewage effluent. | this study describes soil water repellency developed under prolonged irrigation with treated sewage effluent in a semiarid environment. soil surface layer (0-5 cm) and soil profile (0-50 cm) transects were sampled at a high resolution at the close of the irrigation season and rainy winter season. samples from 0- to 5-cm transects were subdivided into 1-cm slices to obtain fine scale resolution of repellency and organic matter distribution. extreme to severe soil water repellency in the 0- to 5-c ... | 2005 | 16151242 |
| biological activity of carotenoids in red paprika, valencia orange and golden delicious apple. | carotenoid fractions were extracted from red paprika, valencia orange peel and the peel of golden delicious apple. thus, hypophasic carotenoids of paprika (pm1), orange (pm3) and apple (pm4), and epiphasic extractions of paprika (pm2) and apple (pm5) were obtained by extraction, saponification and partition between meoh-h(2)o (9:1) (hypophasic) and hexane (epiphasic). a high content of capsanthin was quantified in hypophasic carotenoids (pm1) from red spice paprika, whereas the hypophasic fracti ... | 2005 | 16177974 |
| genetic transformation of mature citrus plants. | most woody fruit species have long juvenile periods that drastically prolong the time required to analyze mature traits. evaluation of characteristics related to fruits is a requisite to release any new variety into the market. because of a decline in regenerative and transformation potential, genetic transformation procedures usually employ juvenile material as the source of plant tissue, therefore resulting in the production of juvenile plants. direct transformation of mature material could en ... | 2005 | 15310921 |
| biotransformation of citrus aromatics nootkatone and valencene by microorganisms. | biotransformations of the sesquiterpene ketone nootkatone from the crude drug alpiniae fructus and grapefruit oil, and the sesquiterpene hydrocarbon valencene from valencia orange oil were carried out with microorganisms such as aspergillus niger, botryosphaeria dothidea, and fusarium culmorum to afford structurally interesting metabolites. their stereostructures were established by a combination of high-resolution nmr spectral and x-ray crystallographic analysis and chemical reaction. metabolic ... | 2005 | 16272725 |
| highly efficient production of nootkatone, the grapefruit aroma from valencene, by biotransformation. | nootkatone, the most important and expensive aromatic of grapefruit, decreases the somatic fat ratio, and thus its demand is increasing in the cosmetic and fiber sectors. a sesquiterpene hydrocarbon, (+)-valencene, which is cheaply obtained from valencia orange, was biotransformed by the green algae chlorella species and fungi such as mucor species, botryosphaeria dothidea, and botryodiplodia theobromae to afford nootkatone in high yield. | 2005 | 16272746 |
| a rapid liquid chromatography electrospray ionization mass spectrometry(n) method for evaluation of synephrine in citrus aurantium l. samples. | immature bitter orange fruit and its extracts have been introduced into the market as an alternative to ephedra in weight loss products. however, the safety of the immature bitter orange fruit and its extracts is a debated argument due to the presence of synephrine, a constituent known as a sympathomimetic agent. in this paper, we describe the development of a new, rapid, and simple liquid chromatography-electrospray ionization-tandem mass spectrometry method devoted to the quantitative determin ... | 2005 | 16366666 |
| [chemical composition and bioactive compounds of flour of orange (citrus sinensis), tangerine (citrus reticulata) and grapefruit (citrus paradisi) peels cultivated in venezuela]. | the purpose of this research was to evaluate the chemical composition and some bioactive compounds in the peel's flour of some of the most consumed citrus fruits cultivated in venezuela. chemical composition as well as some trace elements, ascorbic acid, carotenoids dietary fiber, total polyphenols and their antiradical efficiency, using the 2,2-diphenyl-1-picrylhidracyl (dpph) were assessed in the dried peels of orange (citrus sinensis), tangerine (citrus reticulata) and white grapefruit (citru ... | 2005 | 16454058 |
| efficacy of bacillus thuringiensis against phyllocnistis citrella (lepidoptera: phyllocnistidae). | the purpose of this work was to analyze the efficacy of bacillus thuringiensis berliner on the control of phyllocnistis citrella stainton (lepidoptera: phyllocnistidae) in laboratory and field trials. in the laboratory, four b. thuringiensis were used: dipel (commercial formulation) tested at the concentrations of 25 x 10(6) and 25 x 10(8) spores/ml and the isolates k, 6, and 15 (collections of the university of azores) at the concentration of 25 x 10(6) spores/ ml. a surfactant solution of nono ... | 2005 | 16539108 |
| comparative toxicity effect of bush tea leaves (hyptis suaveolens) and orange peel (citrus sinensis) oil extract on larvae of the yellow fever mosquito aedes aegypti. | the ethanolic extracts of the orange peel (citrus sinensis) and bush tea leaves (hyptis suaveolens) were compared for their toxicity effect on the larvae of the yellow fever mosquito aedes aegypti collected from disused tyres beside college of natural sciences building university of agriculture, abeokuta, nigeria. eight graded concentrations, 0.9ppm, 0.8ppm, 0.7ppm, 0.6ppm, 0.5ppm, 0.4ppm, 0.3ppm and 0.2ppm of both plant extracts were tested on the larvae. the mean lethal dose ld10, was 0.15 ppm ... | 2005 | 16941944 |
| biocontrol of aflatoxins b1, b2, g1, g2, and fumonisin b1 with 6,7-dimethoxycoumarin, a phytoalexin from citrus sinensis. | phytoalexins (stress-induced compounds) from citrus sinensis cultivar valencia were screened for antifungal and antimycotoxic activity against a test organism (cladosporium cladosporoides) and mycotoxin-producing fungi fusarium verticillioides and aspergillus parasiticus. the active compound, a member of the coumarin family of compounds, has antifungal and antimycotoxic activities and was chemically identified. high-performance liquid chromatography results indicated that valencia oranges contai ... | 2006 | 16995528 |
| inhibition of aflatoxin b production of aspergillus flavus, isolated from soybean seeds by certain natural plant products. | the inhibitory effect of cowdung fumes, captan, leaf powder of withania somnifera, hyptis suaveolens, eucalyptus citriodora, peel powder of citrus sinensis, citrus medica and punica granatum, neem cake and pongamia cake and spore suspension of trichoderma harzianum and aspergillus niger on aflatoxin b(1) production by toxigenic strain of aspergillus flavus isolated from soybean seeds was investigated. | 2006 | 17032218 |
| polymethoxylated flavones induce ca(2+)-mediated apoptosis in breast cancer cells. | flavonoids, polyphenolic phytochemicals which include flavones and isoflavones, are present in the common human diet. it has been suggested that these compounds may exert anticancer activity; however, the mechanisms involved remain unknown. we have recently shown (sergeev, 2004, biochem biophys res commun 321: 462-467) that isoflavones can activate the novel apoptotic pathway mediated by cellular ca(2+). here, we report that polymethoxyflavones (pmfs) derived from sweet orange (citrus sinensis l ... | 2006 | 17046027 |
| recombinant expression and characterization of a xylella fastidiosa cysteine protease differentially expressed in a nonpathogenic strain. | xylella fastidiosa is a xylem-limited, gram-negative bacterium responsible for citrus variegated chlorosis (cvc) in sweet oranges. in the present study, we present the recombinant expression, purification and characterization of an x. fastidiosa cysteine protease (dubbed xylellain). the recombinant xylellain ((his)xylellain) was able to hydrolyze carbobenzoxy-phe-arg-7-amido-4-methylcoumarin (z-fr-mca) and carbobenzoxy-arg-arg-7-amido-4-methylcoumarin (z-rr-mca) with similar catalytic efficienci ... | 2006 | 16907719 |
| the role of carotenoid cleavage dioxygenases in the regulation of carotenoid profiles during maturation in citrus fruit. | to investigate the relationship between a carotenoid profile and gene expression for carotenoid cleavage dioxygenases, three citrus varieties that exhibit different 9-cis-violaxanthin levels in their juice sacs, satsuma mandarin (citrus unshiu marc.; a variety accumulating a low level of 9-cis-violaxanthin), valencia orange (citrus sinensis osbeck; variety accumulating a high level of 9-cis-violaxanthin), and lisbon lemon (citrus limon burm.f.; a variety accumulating an undetectable level of 9-c ... | 2006 | 16714310 |
| comparison of supercritical fluid chromatography and liquid chromatography for the separation of urinary metabolites of nobiletin with chiral and non-chiral stationary phases. | nobiletin (nob), a polymethoxylated flavone found in sweet orange (citrus sinensis) peel, is currently recognized as a promising anti-inflammatory and anti-tumor agent. it is believed that, by undergoing metabolic biotransformation in vivo, nobiletin is demethylated by hepatic p450 enzymes, yielding multiple hydroxylated metabolites. however, it has not been possible to date to separate the two demethylated nobiletin metabolites, 3'-demethyl-nob and 4'-demethyl-nob (regio-isomers) on reversed-ph ... | 2006 | 16799930 |
| [spatial distribution of oncometopiafacialis (signoret) (hemiptera: cicadellidae) in citrus orchard]. | the spatial distribution of oncometopia facialis (signoret) was studied in sweet orange to elaborate sampling plans and decision-making procedure for the control of this sharpshooter in the field. the samplings were carried out fortnightly in a grid design disposition of 100 points (plants) in citrus orchard, with yellow sticky traps. the average number of o. facialis ranged from 0.35 to 1.17 insects/trap, with the i index varying from 0.89 to 1.82. the fit tests to negative binomial, poisson di ... | 2006 | 17352074 |
| calibration of sap flow estimated by the compensation heat pulse method in olive, plum and orange trees: relationships with xylem anatomy. | the compensation heat pulse method is widely used to estimate sap flow in conducting organs of woody plants. being an invasive technique, calibration is crucial to derive correction factors for accurately estimating the sap flow value from the measured heat pulse velocity. we compared the results of excision and perfusion calibration experiments made with mature olive (olea europaea l. 'manzanilla de sevilla'), plum (prunus domestica l. 'songal') and orange (citrus sinensis (l.) osbeck. 'cadener ... | 2006 | 16510387 |
| quantitative real-time pcr for detection and identification of candidatus liberibacter species associated with citrus huanglongbing. | citrus huanglongbing (hlb, ex greening) is one of the most serious diseases of citrus. different forms of the disease are caused by different candidatus liberobacter species, candidatus liberibacter asiaticus (las), ca. l. africanus (laf) and ca. l. americanus (lam). the pathogen is transmitted by psyllid insects and by budding with contaminated plant materials. the vector psyllid diaphorina citri can transmit both las and lam. establishment of this vector into florida, reports of lam and las in ... | 2006 | 16414133 |
| rapid, specific and quantitative assays for the detection of the endophytic bacterium methylobacterium mesophilicum in plants. | xylella fastidiosa is a xylem-limited bacterium that causes citrus variegated chlorosis disease in sweet orange. there is evidence that x. fastidiosa interacts with endophytic bacteria present in the xylem of sweet orange, and that these interactions, particularly with methylobacterium mesophilicum, may affect disease progress. however, these interactions cannot be evaluated in detail until efficient methods for detection and enumeration of these bacteria in planta are developed. we have previou ... | 2006 | 16266765 |
| potential perchlorate exposure from citrus sp. irrigated with contaminated water. | citrus produced in the southwestern united states is often irrigated with perchlorate-contaminated water. this irrigation water includes colorado river water which is contaminated with perchlorate from a manufacturing plant previously located near the las vegas wash, and ground water from wells in riverside and san bernardino counties of california which are affected by a perchlorate plume associated with an aerospace facility once located near redlands, california. studies were conducted to eva ... | 2006 | 17723376 |
| inheritance and heritability of resistance to citrus leprosis. | abstract the genetic inheritance of resistance to leprosis, the most important viral disease of citrus in brazil, was characterized through the phenotypic assessment of 143 hybrids resulting from crosses between tangor 'murcott' (citrus sinensis x c. reticulata) and sweet orange 'pêra' (c. sinensis), considered to be resistant and susceptible to the disease, respectively. all plants were grafted onto rangpur lime (c. limonia) and inoculated with citrus leprosis virus, cytoplasmic type through th ... | 2006 | 18943497 |
| the effect of lemon, orange and bergamot essential oils and their components on the survival of campylobacter jejuni, escherichia coli o157, listeria monocytogenes, bacillus cereus and staphylococcus aureus in vitro and in food systems. | to investigate the effectiveness of oils and vapours of lemon (citrus limon), sweet orange (citrus sinensis) and bergamot (citrus bergamia) and their components against a number of common foodborne pathogens. | 2006 | 17105553 |
| an effective and low-cost culture medium for isolation and growth of xylella fastidiosa from citrus and coffee plants. | buffered charcoal-yeast extract medium (bcye) has been used for isolation of xylella fastidiosa from citrus (citrus sinensis) and coffee (coffea arabica) plants affected by citrus variegated chlorosis (cvc) and coffee leaf scorch (cls). bcye is composed of aces (2-[2-amino-2oxoethyl) amino]-ethanesulfonic acid) buffer, activated charcoal, yeast extract, l-cysteine, ferric pyrophosphate, and agar. aces buffer is costly and not always commercially available in brazil, and the l-cysteine and ferric ... | 2006 | 17111277 |
| diversity of flies (diptera: tephritidae and lonchaeidae) in organic citrus orchards in the vale do rio caí, rio grande do sul, southern brazil. | this study was carried out in the counties of montenegro and pareci novo located in the region of the vale do rio cai, rio grande do sul, southern brazil, aiming to determine the fruit fly species of tephritidae and lonchaeidae that occur in organic orchards of sweet orange [citrus sinensis (l.) osb.] cultivar céu, and murcott tangor (citrus reticulata blanco x c. sinensis), during the fruit ripening stages in 2003 and 2004. eight mcphail traps baited with integral grape juice diluted to 25% wer ... | 2006 | 17144140 |
| the complete chloroplast genome sequence of citrus sinensis (l.) osbeck var 'ridge pineapple': organization and phylogenetic relationships to other angiosperms. | the production of citrus, the largest fruit crop of international economic value, has recently been imperiled due to the introduction of the bacterial disease citrus canker. no significant improvements have been made to combat this disease by plant breeding and nuclear transgenic approaches. chloroplast genetic engineering has a number of advantages over nuclear transformation; it not only increases transgene expression but also facilitates transgene containment, which is one of the major impedi ... | 2006 | 17010212 |
| nobiletin: efficient and large quantity isolation from orange peel extract. | it is known that nobiletin possesses anticancer, antiviral and anti-inflammatory activities. recently, the demand for nobiletin in large quantities and high purity has increased. however, conventional normal-phase silica gel chromatography and c(18)-reverse-phase separation methods cannot satisfy the requirement of pure and gram scale nobiletin in a timely manner. in exploring the composition and the biological activities of polymethoxyflavones from sweet orange (citrus sinensis) peel, we develo ... | 2006 | 15999338 |
| gene isolation, analysis of expression, and in vitro synthesis of glutathione s-transferase from orange fruit [citrus sinensis l. (osbeck)]. | glutathione s-transferases (gsts) (ec 2.5.1.18) are ubiquitous enzymes that have a defined role in xenobiotic detoxification, but a deeper knowledge of their function in endogenous metabolism is still lacking. in this work, we isolated the cdnas as well as the genomic clones of orange gsts. having considered gene organization and homology data, we suggest that the isolated gst gene is probably involved in the vacuolar import of anthocyanins. we also found that the blood and blond orange gsts sha ... | 2006 | 17117814 |
| gus gene remains stable in transgenic citrus callus recovered from cryopreservation. | the conservation of transgenic materials is very important, paticularly for their potential future use in crop development. in this study, transgenic callus cultures of 'newhall' navel orange (citrus sinensis osbeck) were cryopreserved by a vitrification method. transgenic calluses survived cryopreservation and recovered under normal culture conditions. the results of pcr (polymerase chain reaction) amplification, southern blotting and sscp (single-strand conformation polymorphism) assay showed ... | 2006 | 14671689 |
| evidence for host plant preference by iphiseiodes quadripilis (acari: phytoseiidae) on citrus. | in this study, we present field and laboratory evidence on the preference of iphiseiodes quadripilis (banks) for grapefruit (citrus paradisi macfadyen) leaves compared with sweet orange (citrus sinensis (l.) osbeck) leaves. this preference was confirmed in four orchards whether leaf samples were taken from either border trees of contiguous grapefruit or sweet orange or interior row trees with both citrus species in adjacent rows. iphiseiodes quadripilis was most abundant in grapefruit trees in s ... | 2006 | 16897564 |
| relationship between ploidy variation of citrus calli and competence for somatic embryogenesis. | this study focuses on the relationship between the genetic variation of calli and the competence for somatic embryogenesis in citrus. the dna content of 35 citrus calli of different genotypes was measured three times by flow cytometry during a period of four years. the results showed that 71.4 % of the genotypes had a progressive increase of varied cells, while those of page tangelo, shamouti sweet orange, russ navel orange and cleopatra decreased; significant difference in the variation degree ... | 2006 | 16875323 |
| [the relationship between activity and gene expression of phenylalanine ammonia-lyase and peel pitting in 'fengjie' navel orange fruits]. | citrus fruit is prone to develop peel pitting during development and storage, which greatly decreases its fresh market value because of the deterioration of the peel. in the present study, we have examined the effect of different temperatures (15 degrees c and 4 degrees c), waxing and mechanical damage on the changes in the activity of phenylalanine ammonia-lyase (pal) and the incidence of peel pitting in 'fengjie' navel orange (citrus sinensis osbeck) fruits. the expression levels of pal2, pal6 ... | 2006 | 16775409 |
| hydroxylated polymethoxyflavones and methylated flavonoids in sweet orange (citrus sinensis) peel. | polymethoxyflavones (pmfs) from citrus genus have been of particular interest because of their broad spectrum of biological activities, including antiinflammatory, anticarcinogenic, and antiatherogenic properties. there have been increasing interests in the exploration of health beneficial properties of pmfs in citrus fruits. therefore, the isolation and characterization of pmfs from sweet orange (citrus sinensis) peel will lead to new applications of the byproducts from orange juice processes a ... | 2006 | 16756344 |
| allergic reaction after ingestion of orange blossom pollen. | a 31-year-old atopic subject with allergic rhinoconjunctivitis with sensitization to several pollens, presented with urticaria and angioedema after ingestion of orange blossom pollen (citrus sinensis). a positive skin prick test for orange blossom pollen extract (bial-aristegui, bilbao, spain) at a concentration of 1.2 mg/ml was obtained. serum specific ige antibodies to orange blossom pollen were shown (unicap pharmacia system, uppsala, sweeden). a conjunctival provocation test was negative wit ... | 2006 | 16689190 |
| analysis of microsatellites in citrus unigenes. | simple sequence repeats (ssrs) were investigated in the unigene sequences from expressed sequence tags (est) of sweet orange (citrus sinensis osbeck), trifoliate orange (poncirus trifoliata raf.) and other citrus species and cultivars. a total of 37 802 citrus unigene sequences corresponding to 23.29 mb were searched, resulting in the identification of 8,218 ssrs. among them there were 4,913 (59.8%) mono-, 1,419 (17.3%) di-, 1,709 (20.8%) tri-, 114 (1.39%) tetra-, 23 (0.28%) penta- and 40 (0.49% ... | 2006 | 16625833 |
| gene characterization, analysis of expression and in vitro synthesis of dihydroflavonol 4-reductase from [citrus sinensis (l.) osbeck]. | dihydroflavonol 4-reductase (dfr, ec 1.1.1.219) catalyzes the reduction of dihydroflavonols to leucoanthocyanins, a key "late" step in the biosynthesis of anthocyanins. in this study we showed that a strong reduction in dfr expression occurs in the non-red orange cultivar (navel and ovale) compared to that of the red orange (tarocco) suggesting that the enzyme could be involved in the lack of production of anthocyanins. therefore, we isolated and compared the cdnas, the genomic clones, as well a ... | 2006 | 16524606 |
| germin-like protein cit s 1 and profilin cit s 2 are major allergens in orange (citrus sinensis) fruits. | oranges are clinically relevant allergenic foods. to date, orange allergens have not been characterized in detail. the study is aimed at analyzing the sensitization profile in orange-sensitized subjects with and without clinical allergy, and to identify orange allergens. fifty-six sensitized subjects with self-reported reactions to orange were grouped into reactors (anaphylaxis or multiple episodes of immediate reactions and/or positive challenge tests) and non-reactors (negative open food chall ... | 2006 | 16521162 |
| stomatal oscillations in orange trees under natural climatic conditions. | stomatal oscillations have been reported in many plant species, but they are usually induced by sudden step changes in the environment when plants are grown under constant conditions. this study shows that in navel orange trees (citrus sinensis) pronounced stomatal oscillations occur and persist under natural climatic conditions. | 2006 | 16478765 |
| fruit coloration difference between fengwan, a late-maturing mutant and its original cultivar fengjie72-1 of navel orange (citrus sinensis osbeck). | fruit color is one of the most important external quality traits. mutants with different color are useful for the study of regulating mechanism of coloration progress. a novel mutant, fengwan, derived from fengjie72-1 navel orange (citrus sinensis l. osbeck), has the distinctive characteristic of the coloration delay of 30 d or so, with the change in total soluble solid (tss) and the ratio of tss/acidity. in order to understand the mechanism underlying the difference of coloration between the mu ... | 2006 | 16477128 |
| mining and characterizing microsatellites from citrus ests. | freely available computer programs were arranged in a pipeline to extract microsatellites from public citrus est sequences, retrieved from the ncbi. in total, 3,278 bi- to hexa-type ssr-containing sequences were identified from 56,199 citrus ests. on an average, one ssr was found per 5.2 kb of est sequence, with the tri-nucleotide motifs as the most abundant. primer sequences flanking ssr motifs were successfully identified from 2,295 citrus ests. among those, a subset (100 pairs) were synthesiz ... | 2006 | 16474971 |
| vacuolar citrate/h+ symporter of citrus juice cells. | we have isolated a cdna, designated citrus sinensis citrate transporter 1 cscit1 encoding a novel vacuolar citrate/symporter. immunoblots using antibodies raised against cscit1 showed that the protein is localized to the juice sac cell vacuoles. the highest expression of cscit1 and the amount of protein in the juice sac cell vacuoles coincided with the developmental stage at which the vacuolar citrate content began declining with the concomitant increase in vacuolar ph. vacuoles from sacharomyce ... | 2006 | 16440212 |
| liquid chromatography/mass spectrometry and liquid chromatography/nuclear magnetic resonance as complementary analytical techniques for unambiguous identification of polymethoxylated flavones in residues from molecular distillation of orange peel oils (citrus sinensis). | liquid chromatography/mass spectrometry and liquid chromatography/nuclear magnetic resonance techniques with ultraviolet/diode array detection were used as complementary analytical tools for the reliable identification of polymethoxylated flavones in residues from molecular distillation of cold-pressed peel oils of citrus sinensis. after development of a liquid chromatographic separation procedure, the presence of several polymethoxy flavones such as sinensetin, nobiletin, tangeretin, quercetoge ... | 2006 | 16417279 |
| cloning and characterization of two 9-cis-epoxycarotenoid dioxygenase genes, differentially regulated during fruit maturation and under stress conditions, from orange (citrus sinensis l. osbeck). | there is now biochemical and genetic evidence that oxidative cleavage of cis-epoxycarotenoids by 9-cis-epoxycarotenoid dioxygenase (nced) is the critical step in the regulation of abscisic acid (aba) synthesis in higher plants. the peel of citrus fruit accumulates large amounts of aba during maturation. to understand the regulation of aba biosynthesis in citrus, two full-length cdnas (csnced1 and csnced2) encoding nceds were isolated and characterized from the epicarp of orange fruits (citrus si ... | 2006 | 16396998 |
| pulsed electric fields versus thermal treatment: equivalent processes to obtain equally acceptable citrus juices. | pulsed electric field treatment has been claimed to produce more acceptable chilled citrus juices than those obtained by conventional thermal treatment. the pectin methylesterase activity and the acceptability of nine juices obtained from clementine mandarins, valencia oranges, and ortanique fruits (hybrid of mandarin and orange), untreated, pasteurized (85 degrees c for 10 s), and treated by pulsed electric fields (25 kv/cm for 330 micros), were evaluated. the treatments, selected to reach a si ... | 2006 | 16924935 |
| [effects of exogenous aba and ga3 on sugar concentration in flesh of cara cara navel orange]. | the concentration of glucose, fructose, sucrose and total sugar were determined after exogenous aba and ga3 treatment during young period of fruit and before fruit coloring in flesh of cara cara navel orange. the results showed that 10 mg x l(-1) aba treatment improved glucose, fructose and total sugar concentration significantly or very significantly, 50 mg x l(-1) aba treatment improved sucrose concentration very significantly, but 100 mg x l(-1) aba treatment reduced glucose concentration ver ... | 2007 | 18260446 |
| [near infrared diffuse reflectance spectroscopy for rapid analysis of soluble solids content in navel orange]. | the potential of using visible and near infrared diffuse reflectance spectroscopy to assess soluble solids content (ssc) of intact navel orange was examined. a total 40 samples were used to develop the calibration and prediction models. nir spectral data were collected in the spectral region between 350 and 2 500 nm and their second derivative spectra were used for the present study. different scattering correction algorithms (no preprocessing and multiplicative scattering correction (msc)) were ... | 2007 | 18260391 |
| the debittering of navel orange juice using polymeric films. | in order to better understand and optimize the sorption of limonin (the major navel orange juice bitter principle) by various plasticized polymeric films, a sorption and plasticizer migration study was carried out using high-performance liquid chromatography (hplc) and gas chromatography (gc). low molecular weight (lmw) poly (vinyl chloride) (pvc) plasticized with dioctyl adipate (doa) gave the best results for both limonin sorption and low doa migration. thick films did not significantly sorb m ... | 2007 | 17995766 |
| [visible transmittance techniques for rapid analysis of sugar content of fresh navel orange juices]. | the feasibility of visible spectroscopic technology for rapid quantifying sugar content (sc) of navel orange fresh juices was investigated by means of spectral transmittance technique. a total of 55 juice samples were used to develop the calibration and prediction models. calibration models based on different spectral ranges and different spectral pretreatment were compared in the precent research. performance of different models was investigated in terms of root mean square errors of prediction ... | 2007 | 17554924 |
| [construction and preliminary analysis of subtractive library for peel pitting of 'fengjie' navel orange fruit]. | in this study, the pitted peel and non-pitted peel of 'fengjie' navel orange fruits were used as experimental materials to construct and screen the peel pitting related genes by suppression subtractive hybridization (ssh). the results showed that suppression subtractive hybridization was very effective. a cdna library of differentially expressed genes was constructed. the library included about 200 clones with an average insert size of around 300 bp. part of the positive clones were picked up ra ... | 2007 | 17287572 |
| a screening method for the identification of glycosylated flavonoids and other phenolic compounds using a standard analytical approach for all plant materials. | a screening method was developed for the systematic identification of glycosylated flavonoids and other phenolic compounds in plant food materials based on an initial, standard analytical method. this approach applies the same analytical scheme (aqueous methanol extraction, reverse phase liquid chromatographic separation, and diode array and mass spectrometric detection) to every sample and standard. this standard approach allows the cross-comparison of compounds in samples, standards, and plant ... | 2007 | 17256956 |
| selection of colour of sticky trap for monitoring adult bean thrips, caliothrips fasciatus (thysanoptera: thripidae). | adult bean thrips, caliothrips fasciatus (pergande), overwintering inside the navel of navel oranges shipped from california to australia, are an actionable pest for the importing country, i.e. infested lots are fumigated with methyl bromide. strict quarantine regulations regarding c. fasciatus prompted studies on the best colour sticky trap that might be used to monitor for bean thrips populations in the vicinity of california citrus groves prior to harvesting fruit for export. preliminary expe ... | 2007 | 17125151 |
| a comparison of anion concentration in surficial groundwater sampled from two types of water quality monitoring wells. | groundwater sampling for monitoring the presence and concentration of contaminants can be done using either depth integrated monitoring wells (mw) or depth specific multi-level sampling (mls) wells. depth specific multi-level sampling wells (mls) are cost-effective, easy to install, and provide very detailed information about the vertical gradient in contaminant concentration. in contrast the mw sampling provides information on the presence of contaminant over large representative area. this stu ... | 2007 | 17129947 |
| [effects of foliage spraying sodium bisulfite on photosynthetic physiological characteristics of citrus sinensis with sulphur deficiency]. | the study with solution culture showed that citrus sinensis plants with sulphur deficiency and with sulphur deficiency plus foliage spraying sodium bisulfite ( nahso3 ) halfway had notably decreased photosynthetic pigment content, soluble protein content, net photosynthetic rate (pn), initial fluorescence (fo) , maximum fluorescence (fm) , photochemical efficiency (fv/fm) and electron transmit rate (etr) , compared with those having normal sulphur supply. after 35 days of growth, their photosynt ... | 2007 | 17450735 |
| whole-tree level water balance and its implications on stomatal oscillations in orange trees [citrus sinensis (l.) osbeck] under natural climatic conditions. | sustained cyclic oscillations in stomatal conductance, leaf water potential, and sap flow were observed in young orange trees growing under natural conditions. the oscillations had an average period of approximately 70 min. water uptake by the roots and loss by the leaves was characterized by large time lags which led to imbalances between water supply and demand in the leaves. the bulk of the lag in response between stomatal movements and the upstream water balance resided downstream of the bra ... | 2007 | 17443016 |
| isolation and syntheses of polymethoxyflavones and hydroxylated polymethoxyflavones as inhibitors of hl-60 cell lines. | fifteen polymethoxyflavones (pmfs) and hydroxylated pmfs were isolated from sweet orange (citrus sinensis) peel extract and synthesized to investigate their biological activity. all obtained compounds were tested in hl-60 cancer cell proliferation and apoptosis induction assays. while some pmfs and hydroxylated pmfs had moderate anti-carcinogenic activities, 5-hydroxy-6,7,8,3',4'-pentamethoxyflavone and 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone showed strong inhibitory activities against the pr ... | 2007 | 17391969 |
| binomial sampling to estimate rust mite (acari: eriophyidae) densities on orange fruit. | binomial sampling based on the proportion of samples infested was investigated for estimating mean densities of citrus rust mite, phyllocoptruta oleivora (ashmead), and aculops pelekassi (keifer) (acari: eriophyidae), on oranges, citrus sinensis (l.) osbeck. data for the investigation were obtained by counting the number of motile mites within 600 sample units (each unit a 1-cm2 surface area per fruit) across a 4-ha block of trees (32 blocks total): five areas per 4 ha, five trees per area, 12 f ... | 2007 | 17370833 |
| plant somatic hybrid cytoplasmic dna characterization by single-strand conformation polymorphism. | unlike maternal inheritance in sexual hybridization, plant somatic hybridization allows transfer, mixing and recombination of cytoplasmic genomes. in addition to the use of somatic hybridization in plant breeding programs, application of this unique tool should lead to a better understanding of the roles played by the chloroplastic and mitochondrial genomes in determining agronomically important traits. the nucleotide sequences of cytoplasmic genomes are much more conserved than those of nuclear ... | 2007 | 17331897 |
| [seasonal variation of immature stages of phyllocnistis citrella stainton (lepidoptera: gracillariidae) in citrus sinensis orchards under two management systems]. | phyllocnistis citrella stainton is considered an important pest of citrus, causing both direct (reduction on the photosynthetic area) and indirect damage (facilitation of invasion by bacteria that cause citrus canker). the lack of information about the population dynamics of p. citrella, considering the cultivation systems and varieties grown in citrus orchards in rio grande do sul, brazil, motivated this study. it aimed to evaluate the seasonal variation of immature stages of p. citrella, from ... | 2007 | 17273716 |
| hydraulic characteristics and water relations in pigment-less mutant shoots of an orange tree. | phenotypes more or less deficient in photosynthetic pigments show reduced productivity. not much is known, however, about the influence of pigment-less twigs on the water balance of whole trees. we studied the water relations and hydraulic properties of normal and pigment-less (white) and 1-year-old shoots of 12-year-old citrus sinensis l. trees. compared with green leaves, white leaves showed a pronounced deficiency of pigments, higher stomatal density, the absence of chloroplasts in the guard ... | 2007 | 17241963 |
| expression of phytoene synthase gene (psy) is enhanced during fruit ripening of cara cara navel orange (citrus sinensis osbeck). | citrus is an important fruit crop as regards accumulation of carotenoids. in plant carotenoid biosynthesis, phytoene synthase gene (psy) plays a key role in catalyzing the head-to-head condensation of geranylgeranyl diphosphate molecules to produce colorless phytoene. in the present paper, we reported the phytoene contents determination and characterization of psy during fruit ripening of "washington" navel orange and its red-fleshed mutant "cara cara". results showed that phytoene was exclusive ... | 2007 | 17226057 |
| cire1, a novel transcriptionally active ty1-copia retrotransposon from citrus sinensis. | ltr retrotransposons (ltr-rtns) are widespread constituents of eukaryote genomes, particularly plant genomes. although ltr-rtns from plants were thought to be transcriptionally silent in somatic tissues, evidences of activity under certain conditions are available for some of them. in order to investigate ltr-rtns in the citrus sinensis genome, we analysed them by pcr using degenerate primers corresponding to highly conserved domains. all elements of the two types of ltr-rtn comprise about 23% o ... | 2007 | 17216224 |