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protective effects of orange (citrus sinensis l.) peel aqueous extract and hesperidin on oxidative stress and peptic ulcer induced by alcohol in rat.massive alcohol drinking can lead to gastric ulcer. in the present study we investigated the gastroprotective effect of citrus sinensis peel aqueous extract (cspe) and hesperidin (h) in ethanol (etoh) induced oxidative stress and peptic ulcer in rats.201728806973
effects of preheating and storage temperatures on aroma profile and physical properties of citrus-oil emulsions.citrus oils are used as good carrier oil for emulsion fabrication due to their special flavor and various health-promoting functions. in this study, the effects of preheating temperature (30, 40, 50, 60, and 70 °c) and storage temperature (4, 25, and 37 °c) on aroma profiles and physical properties of three citrus-oil (i.e., mandarin, sweet orange, and bergamot oils) emulsions were systematically investigated for the first time. the results demonstrated the significant impact of temperature on a ...201728820942
anticoagulant and antithrombotic effects of chemically sulfated fucogalactan and citrus pectin.citrus pectin (cp14) from citrus sinensis, and a fucogalactan (e) and a glucan (g16) from agaricus bisporus were isolated and structurally characterized. cp14 was constituted by (1→4)-linked α-d-galpa units, e was composed by a (1→6)-linked α-d-galp main-chain, partially substituted at o-2 by non-reducing end-units of α-l-fucp or α-d-galp, and partially methylated at o-3, whereas g16 was composed of (1→6)-linked β-d-glcp units. the polysaccharides were sulfated giving rise to cp14s, esl and g16s ...201728821126
antioxidant and anticancer activities of essential oil from gannan navel orange peel.china is one of the leading producers of citrus in the world. gannan in jiangxi province is the top navel orange producing area in china. in the present study, an essential oil was prepared by cold pressing of gannan navel orange peel followed by molecular distillation. its chemical composition was analyzed by gc-ms. twenty four constituents were identified, representing 97.9% of the total oil. the predominant constituent was limonene (74.6%). the anticancer activities of this orange essential o ...201728829378
fresh-squeezed orange juice properties before and during in vitro digestion as influenced by orange variety and processing method.this study aims to analyze the influence of processing and orange variety on initial quality, antioxidant activity, total polyphenol content (tpc), and ascorbic acid content of fresh-squeezed orange juice during in vitro digestion. fresh-squeezed orange juice was made from fukumoto navel, lane late navel, olinda nucellar valencia, and campbell valencia oranges, and was processed thermally and nonthermally. antioxidant activity (frap and abts assays), tpc (folin-ciocalteu method), and ascorbic ac ...201728833183
microrna-mediated responses to long-term magnesium-deficiency in citrus sinensis roots revealed by illumina sequencing.magnesium (mg)-deficiency occurs most frequently in strongly acidic, sandy soils. citrus are grown mainly on acidic and strong acidic soils. mg-deficiency causes poor fruit quality and low fruit yield in some citrus orchards. for the first time, we investigated mg-deficiency-responsive mirnas in 'xuegan' (citrus sinensis) roots using illumina sequencing in order to obtain some mirnas presumably responsible for citrus mg-deficiency tolerance.201728836935
two of a kind but different: luminescent carbon quantum dots from citrus peels for iron and tartrazine sensing and cell imaging.citrus sinensis and citrus limon peels were used to synthesize two different kinds of carbon quantum dots (cqds) via an unsophisticated and inexpensive carbonization procedure. the proposed synthesis is straightforward and adheres to the principles of green chemistry since no organic solvents are used and no toxic by-products are formed, while the residual resources employed facilitate the large scale synthesis of dots. the citrus sinensis and citrus limon peels are proved to be excellent precur ...201728841995
expression patterns of flowering genes in leaves of 'pineapple' sweet orange [citrus sinensis (l.) osbeck] and pummelo (citrus grandis osbeck).in citrus the transition from juvenility to mature phase is marked by the capability of a tree to flower and fruit consistently. the long period of juvenility in citrus severely impedes the use of genetic based strategies to improve fruit quality, disease resistance, and responses to abiotic environmental factors. one of the genes whose expression signals flower development in many plant species is flowering locus t (ft).201728854897
characterization and metabolic diversity of flavonoids in citrus species.flavonoids are widely distributed in plants and play important roles in many biological processes. citrus fruits are rich dietary sources of flavonoids. however, there have been very few reports about the comprehensive metabolic profile and natural diversity of flavonoids in different tissues of various citrus cultivars. in this study, based on the 7416 metabolic signals detected with non-targeted metabolomics approach, principal component analysis revealed the flavedo has the largest difference ...201728874745
genomic analysis of terpene synthase family and functional characterization of seven sesquiterpene synthases from citrus sinensis.citrus aroma and flavor, chief traits of fruit quality, are derived from their high content in essential oils of most plant tissues, including leaves, stems, flowers, and fruits. accumulated in secretory cavities, most components of these oils are volatile terpenes. they contribute to defense against herbivores and pathogens, and perhaps also protect tissues against abiotic stress. in spite of their importance, our understanding of the physiological, biochemical, and genetic regulation of citrus ...201728883829
phylogenetic study of the colletotrichum species on imported citrus fruits uncovers a low diversity and a new species in the colletotrichum gigasporum complex.colletotrichum species associated with citrus fruits are fragmentarily known and it lacks accordingly accurate information on the diversity carried alongside the trade of these commodities from producer countries to europe. in this study, we investigated the molecular phylogenetic diversity, colonisation, and prevalence of colletotrichum isolated from asymptomatic and diseased tissues of nine citrus fruit species from 17 geographically diverse countries. totally 454 isolates were morphoculturall ...201728889910
antimutagenic and antioxidant activity of the essential oils of citrus sinensis and citrus latifolia.the essential oils of citrus sinensis and citrus latifolia showed antimycotic activity against candida spp. isolated from the oral cavity; they are neither mutagenic on the ames test nor cytotoxic. their main components are r-(+)-limonene, β-thujene, α-myrcene and γ-terpinene. the aim of this work was to evaluate their antimutagenic and antioxidant capacities. antimutagenic properties were evaluated against mnng and enng on s. typhimurium ta100; against 2aa on strain ta98 and in front of 4nqo an ...201728904369
comparative transcriptome analysis during early fruit development between three seedy citrus genotypes and their seedless mutants.identification of genes with differential transcript abundance (gdta) in seedless mutants may enhance understanding of seedless citrus development. transcriptome analysis was conducted at three time points during early fruit development (phase 1) of three seedy citrus genotypes: fallglo (bower citrus hybrid (citrus reticulata×c. reticulata×c. paradisi)×temple (c. reticulata×c. sinensis)), grapefruit (c. paradisi), pineapple sweet orange (c. sinensis), and their seedless mutants. seed abortion in ...201728904803
antibacterial and antibiotic-modifying activity of methanol extracts from six cameroonian food plants against multidrug-resistant enteric bacteria.the present work was designed to investigate the antibacterial activities of methanol extracts from six cameroonian edible plants and their synergistic effects with some commonly used antibiotics against multidrug-resistant (mdr) gram-negative bacteria expressing active efflux pumps. the extracts were subjected to qualitative phytochemical screening and the microdilution broth method was used for antibacterial assays. the results of phytochemical tests indicate that all tested crude extracts con ...201728904944
long-term manganese-toxicity-induced alterations of physiology and leaf protein profiles in two citrus species differing in manganese-tolerance.manganese (mn)-intolerant 'sour pummelo' (citrus grandis) and mn-tolerant 'xuegan' (citrus sinensis) seedlings were irrigated for 17 weeks with 2 (control) or 600μm (mn-toxicity or -excess) mnso4. c. sinensis had higher mn-tolerance than c. grandis, as indicated by the higher photosynthesis rates in mn-excess c. sinensis leaves. under mn-toxicity, mn levels were similar between c. sinensis and c. grandis roots, but lower in c. sinensis leaves than in c. grandis leaves. this might be responsible ...201728910703
co-infection of sweet orange with severe and mild strains of citrus tristeza virus is overwhelmingly dominated by the severe strain on both the transcriptional and biological levels.citrus tristeza is one of the most destructive citrus diseases and is caused by the phloem-restricted closterovirus, citrus tristeza virus. mild strain ctv-b2 does not cause obvious symptoms on indicators whereas severe strain ctv-b6 causes symptoms, including stem pitting, cupping, yellowing, and stiffening of leaves, and vein corking. our laboratory has previously characterized changes in transcription in sweet orange separately infected with ctv-b2 and ctv-b6. in the present study, transcript ...201728912786
the effect of viroid infection of citrus trees on the amoebicidal activity of 'maltese half-blood' (citrus sinensis) against trophozoite stage of acanthamoeba castellanii neff.in order to promote a local tunisian product, this study was designed to examine, for the first time, the anti-acanthamoeba activity (acanthamoeba castellanii neff) of the essential oils of tunisian citrus sinensis peels (maltese half-blood) and the effect of viroid plant infection on this activity. to do so, three samples of peels' essential oils were studied: from a healthy plant (control), a plant inoculated with citrus exocortis viroid (cevd) and one inoculated with hot stunt cachexia viroid ...201728916460
[study on hplc fingerprint and chemical constituent difference of different species of aurantii fructus immaturus].this study is to establish an hplc fingerprint by hplc-dad method and simultaneous quantitative analysis of 17 components of 18 batches of citrus aurantium and 10 batches of c. sinensis. the separation was performed on an agilent poroshell 120 sb-c₁₈ (4.6 mm×100 mm,2.7 μm) column with the gradient elution of methanol-0.1% formic acid water, the flow was 0.6 ml•min⁻¹. the detection wavelength was set at 318 nm. the column temperature was maintained at 30 ℃. the data calculation was performed with ...201628920382
photodynamic inactivation of conidia of the fungus colletotrichum abscissum on citrus sinensis plants with methylene blue under solar radiation.antimicrobial photodynamic treatment (apdt) is a promising light based approach to control diseases caused by plant-pathogenic fungi. in the present study, we evaluated the effects of apdt with the phenothiazinium photosensitizer methylene blue (mb) under solar radiation on the germination and viability of conidia of the pathogenic fungus colletotricum abscissum (former colletotrichum acutatum sensu lato). experiments were performed both on petals and leaves of sweet orange (citrus sinensis) in ...201728941778
control of citrus huanglongbing via trunk injection of plant defense activators and antibiotics.citrus huanglongbing (hlb) or greening is a devastating disease of citrus worldwide and no effective control measure is currently available. plant defense activators environmentally friendly compounds capable of inducing resistance against many plant pathogens. earlier studies showed that foliar spray of plant defense inducers could slow down hlb disease progress. in this study, eight plant defense activators and three antibiotics were evaluated in three field trials for their effect to control ...201828945516
effect of aromatherapy via inhalation on the sleep quality and fatigue level in people undergoing hemodialysis.the most common problems in hemodialysis patients are sleep disorders and fatigue. this randomized-controlled experimental study was conducted to determine the effect of aromatherapy applied by inhalation on sleep quality and fatigue level in hemodialysis patients. the study was completed in five hemodialysis centers settled in two provinces with 27 intervention group patients and 35 controls, being totally 62 patients, recruited with simple randomization. ethical approval, informed consent from ...201728985917
transcription factor citerf71 activates the terpene synthase gene cittps16 involved in the synthesis of e-geraniol in sweet orange fruit.the unique flavor of citrus fruit depends on complex combinations of soluble sugars, organic acids, and volatile compounds. the monoterpene e-geraniol is an important volatile, contributing to flavor in sweet orange (citrus sinensis osbeck). moreover, antifungal activity of e-geraniol has also been observed. however, the terpene synthase (tps) responsible for its synthesis has not been identified in sweet orange. terpene synthase 16 (cittps16) was shown to catalyze synthesis of e-geraniol in vit ...201728992329
chemical composition of the essential oils isolated from peel of three citrus species and their mosquitocidal activity against culex pipiens.three essential oils (eos) were isolated from the peel of citrus fruits citrus reticulata l., citrus reticulata chinase blanco and citrus sinensis (l.) osbeck (family: rutaceae) and evaluated against culex pipiens l.1758 (family: culicidae). chemical composition indicated that the eos were rich in essential phytochemicals including hydrocarbons, monoterpenes and sesquiterpenes. these constituents revealed some variability among the oils displaying interesting chemotypes limonene (35-51%), 1r-α-p ...201729017021
selection of reference genes for expression studies in diaphorina citri (hemiptera: liviidae).the asian citrus psyllid, diaphorina citri kuwayama (hemiptera: liviidae), is considered the main vector of the bacteria associated with huanglongbing, a very serious disease that has threatened the world citrus industry. the absence of efficient control management protocols, including a lack of resistant cultivars, has led to the development of different approaches to study this pathosystem. the production of resistant genotypes relies on d. citri gene expression analyses by rt-qpcr to assess c ...201729029285
effects of transgenic expression of brevibacterium linens methionine gamma lyase (mgl) on accumulation of tylenchulus semipenetrans and key aminoacid contents in carrizo citrange.carrizo transgenic plants overexpressing methionine-gamma-lyase produced dimethyl sulfide. the transgenic plants displayed more resistance to nematode attacks (tylenculus semipenetrans) and may represent an innovative strategy for nematode control. tylenchulus semipenetrans is a nematode pest of many citrus varieties that causes extensive damage to commercial crops worldwide. carrizo citrange vr. (citrus sinensis l. usb × poncirus trifoliate l. raf) plants overexpressing brevibacterium linens me ...201729058103
bio-pesticides: new tool for the control of aedes (stegomyia) albopictus (culicidae: diptera) in pakistan.application of plant extracts as mosquito control strategy was practiced from centuries. these are easily available, non-toxic, biodegradable and exhibit broad-spectrum target specific activities against larval stages of mosquitoes.201729062852
mass spectrometry characterization of endophytic bacterium curtobacterium sp. strain er1/6 isolated from citrus sinensis.the bacteria of the genus curtobacterium are usually seen as plant pathogen, but some species have been identified as endophytes of different crops and could as such present a potential for disease control and plant growth promotion. we have therefore applied the desorption electrospray ionization mass spectrometry imaging (desi-msi) in the direct analysis of living curtobacterium sp. strain er1/6 colonies to map the surface metabolites, and electrospray ionization tandem mass spectrometry (esi- ...201829076604
gene coexpression network analysis of fruit transcriptomes uncovers a possible mechanistically distinct class of sugar/acid ratio-associated genes in sweet orange.the ratio of sugars to organic acids, two of the major metabolites in fleshy fruits, has been considered the most important contributor to fruit sweetness. although accumulation of sugars and acids have been extensively studied, whether plants evolve a mechanism to maintain, sense or respond to the fruit sugar/acid ratio remains a mystery. in a prior study, we used an integrated systems biology tool to identify a group of 39 acid-associated genes from the fruit transcriptomes in four sweet orang ...201729084509
transcriptional analysis of sweet orange trees co-infected with 'candidatus liberibacter asiaticus' and mild or severe strains of citrus tristeza virus.citrus worldwide is threatened by huanglongbing (hlb) and tristeza diseases caused by 'candidatus liberibacter asiaticus' (calas) and citrus tristeza virus (ctv). although the pathogens are members of the α-proteobacteria and closteroviridae, respectively, both are restricted to phloem cells in infected citrus and are transmitted by insect vectors. the response of sweet orange to single infection by either of these two pathogens has been characterized previously by global gene expression analysi ...201729089035
oviposition of fruit flies (diptera: tephritidae) and its relation with the pericarp of citrus fruits.fruit flies (diptera: tephritidae) represent a threat to fruit growing worldwide, mainly the citrus culture, however, biological studies show that fruit flies are not perfectly adapted to this host. this study investigated oviposition of anastrepha fraterculus (wiedemann, 1830) and ceratitis capitata (wiedemann, 1824) and its relation with the pericarp of citrus fruits. we evaluated the relationship between depth of oviposition of a. fraterculus and c. capitata and epicarp thickness of orange [c ...201729091115
natural occurrence of aldol condensation products in valencia orange oil.cold pressed orange oils contain predominantly d-limonene (approximately 95%) and various other lower concentration monoterpenes, sesquiterpenes, sinensals plus 3 key aliphatic aldehydes: hexanal, octanal. and decanal. the aldol self-condensation products or "dimers" for each aldehyde have been postulated as being present at low concentrations in the oil. however, to date only the hexanal dimer has been previously reported. in this paper, cold pressed valencia orange oil was fractionally distill ...201729095499
recent advances in understanding xanthomonas citri subsp. citri pathogenesis and citrus canker disease management.taxonomic status: bacteria; phylum proteobacteria; class gammaproteobacteria; order xanthomonadles; family xanthomonadaceae; genus xanthomonas; species xanthomonas citri subsp. citri (xcc). host range: compatible hosts vary in their susceptibility to cc, with grapefruit, lime, and lemon being most susceptible, sweet orange being moderately susceptible, and kumquat and calamondin being among the least susceptible (gottwald et al., 1993). microbiological properties: xcc is a rod-shaped (1.5 - 2.0 ...201729105297
green synthesis of capped gold nanoparticles and their effect on gram-positive and gram-negative bacteria.we report synthesis of capped gold nanoparticles (c-aunps) of ≈20-30 nm by reducing haucl4 with flower and leaf extracts of ocimum tenuiflorum, leaves of azadirachta indica and mentha spicata and peel of citrus sinensis plants.201729134123
[insecticidal action of synthetic girgensohnine analogues and essential oils on rhodnius prolixus (hemiptera: reduviidae)].the alkaloid girgensohnine has been used as a natural model in the synthesis of new alkaloid-like alpha-aminonitriles with insecticidal effect against disease vectors.201729161477
citrus essential oils inhalation by mice: behavioral testing, gcms plasma analysis, corticosterone, and melatonin levels evaluation.the use of orange essential oils (eos) as a complementary treatment is very common in brazilian popular culture. the levels of melatonin (mel) and corticosterone (cort) hormones were investigated simultaneously, by the luminex™ immunoassay system in mice plasma, after citrus aurantium and citrus sinensis eos inhalation for 30 min. the plasma was analyzed by headspace through gas chromatography coupled to mass spectrometry for investigation of the eo components. mice were submitted to behavioral ...201829168240
repellency potential of essential oils against housefly, musca domestica l.in present study, the essential oils such as mentha piperita (mentha oil, m.o), cymbopogan citratus (lemongrass oil, lg.o), citrus sinensis (orange oil, o.o), and eucalyptus globulus (eucalyptus oil, e.o) were evaluated for repellency against housefly (musca domestica) in a specially designed chamber. further, to study any synergistic effect, essential oil combinations, i.e., m.o + lg.o, m.o + o.o, and m.o + e.o, were screened at 50:50 and 70:30 ratios. the results showed superior repellency of ...201729197060
reproducible genomic dna preparation from diverse crop species for molecular genetic applications.several high-throughput molecular genetic analyses rely on high-quality genomic dna. copurification of other molecules can negatively impact the functionality of plant dna preparations employed in these procedures. isolating dna from agronomically important crops, such as sugarcane, rice, citrus, potato and tomato is a challenge due to the presence of high fiber, polysaccharides, or secondary metabolites. we present a simplified, rapid and reproducible sds-based method that provides high-quality ...201729213298
reprogramming of a defense signaling pathway in rough lemon and sweet orange is a critical element of the early response to 'candidatus liberibacter asiaticus'.huanglongbing (hlb) in citrus infected by candidatus liberibacter asiaticus (clas) has caused tremendous losses to the citrus industry. no resistant genotypes have been identified in citrus species or close relatives. among citrus varieties, rough lemon (citrus jambhiri) has been considered tolerant due to its ability to produce a healthy flush of new growth after infection. the difference between tolerance and susceptibility is often defined by the speed and intensity of a plant's response to a ...201729214028
does the fragrance of essential oils alleviate the fatigue induced by exercise? a biochemical indicator test in rats.to study the effect of the essential oils of citrus sinensis l., mentha piperita l., syzygium aromaticum l., and rosmarinus officinalis l. on physical exhaustion in rats.201729234408
characterization of mycosphaerellaceae species associated with citrus greasy spot in panama and spain.greasy spot of citrus, caused by zasmidium citri-griseum (= mycosphaerella citri), is widely distributed in the caribbean basin, inducing leaf spots, premature defoliation, and yield loss. greasy spot-like symptoms were frequently observed in humid citrus-growing regions in panama as well as in semi-arid areas in spain, but disease aetiology was unknown. citrus-growing areas in panama and spain were surveyed and isolates of mycosphaerellaceae were obtained from citrus greasy spot lesions. a sele ...201729236789
differentiation between flavors of sweet orange (citrus sinensis) and mandarin (citrus reticulata).pioneering investigations referring to citrus flavor have been intensively conducted. however, the characteristic flavor difference between sweet orange and mandarin has not been defined. in this study, sensory analysis illustrated the crucial role of aroma in the differentiation between orange flavor and mandarin flavor. to study aroma, valencia orange and lb8-9 mandarin were used. their most aroma-active compounds were preliminarily identified by aroma extract dilution analysis (aeda). quantit ...201829237265
influence of natural and synthetic vitamin c (ascorbic acid) on primary and secondary metabolites and associated metabolism in quinoa (chenopodium quinoa willd.) plants under water deficit regimes.phytoextracts are being widely used these days as a source of bioactive compounds for mitigating the harmful effects of abiotic stresses including drought stress. in this study, it was assessed how far foliar applied pure synthetic ascorbic acid (asa) or natural sweet orange juice (oj) enriched with asa could mitigate the drought stress induced adverse effects on growth and some key metabolic processes in quinoa (chenopodium quinoa willd.; cultivar v9) plants. two weeks old quinoa seedlings were ...201729248677
draft sequencing of the heterozygous diploid genome of satsuma (citrus unshiu marc.) using a hybrid assembly approach.satsuma (citrus unshiu marc.) is one of the most abundantly produced mandarin varieties of citrus, known for its seedless fruit production and as a breeding parent of citrus. de novo assembly of the heterozygous diploid genome of satsuma ("miyagawa wase") was conducted by a hybrid assembly approach using short-read sequences, three mate-pair libraries, and a long-read sequence of pacbio by the platanus assembler. the assembled sequence, with a total size of 359.7 mb at the n50 length of 386,404 ...201729259619
comparative transcriptome and proteome profiling of two citrus sinensis cultivars during fruit development and ripening.transcriptome and proteome analyses on fruit pulp from the blood orange 'zaohong' and the navel orange 'twenty-first century' were performed to study citrus sinensis quality-related molecular changes during consecutive developmental periods, including young fruit, fruit-coloring onset and fruit delayed-harvest for two months, during which fruit remained on the trees.201729268697
juice volatile composition differences between valencia orange and its mutant rohde red valencia are associated with carotenoid profile differences.a targeted approach using hs-spme-gc-ms was performed to investigate volatile compounds of ordinary valencia (val) and its more deeply colored mutant rohde red valencia orange (rrv) at different developmental stages. fifty-six volatile components classified into six chemical groups were quantified. the individual volatile compounds in each group were significantly influenced by the genotype and harvest date. cluster analysis suggested two principal clusters. cluster i included fruits of val and ...201829287364
comparative transcriptome analysis of two citrus germplasms with contrasting susceptibility to phytophthora nicotianae provides new insights into tolerance mechanisms.host perception of phytophthora nicotianae switching to necrotrophy is fundamental for disease tolerance of citrus. it involves an hr-like response, strengthening of the cell wall structure and hormonal signaling. stem rot caused by p. nicotianae is a worldwide disease of several important crops, including citrus. given the growing awareness of chemical fungicides drawbacks, genetic improvement of citrus rootstocks remains the best alternative. however, the molecular basis underlying the success ...201729290008
spatial distribution and sampling plans with fixed level of precision for citrus aphids (hom., aphididae) on two orange species.aphis spiraecola patch, aphis gossypii glover, and toxoptera aurantii boyer de fonscolombe are three important aphid pests of citrus orchards. in this study, spatial distributions of the aphids on two orange species, satsuma mandarin and thomson navel, were evaluated using taylor's power law and iwao's patchiness. in addition, a fixed-precision sequential sampling plant was developed for each species on the host plant by green's model at precision levels of 0.25 and 0.1. the results revealed tha ...201829365154
metabolic alterations in the nymphal instars of diaphorina citri induced by candidatus liberibacter asiaticus, the putative pathogen of huanglongbing.currently, huanglongbing is the most damaging disease of citrus causing huge economic losses. the disease is caused by the gram-negative bacterium candidatus liberibacter asiaticus (clas). the pathogen is transmitted in a persistent propagative circulative manner within its vector, the asian citrus psyllid, diaphorina citri. exploring the metabolic alteration in the vector may lead to a better understanding of the nutritional needs of clas and to designing an artificial medium for culturing the ...201829370262
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